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1.
秀珍菇营养配方的试验 总被引：3，自引：0，他引：3
本研究通过三因素三水平的正交试验表明 ,在培养基中添加VB1(2× 10 -6) ,N P K (0 1% ) ,NAA(10× 10 -6) ,最适合秀珍菇菌丝的生长 相似文献
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柑桔采前使用乙烯催熟应慎之又慎,尤其应注意使用浓度。据有关报道,温州蜜柑两成着色时使用500×10~(-6)乙烯利,五成着色时使用300×10~(-6)乙烯利,可提早成熟5～10天。而本文所介绍的事例,乙烯利使用浓度约800×10~(-6),显然偏高。此外,为防止使用乙烯后发生落叶落果,最好与2,4-D配合使用。另据日本报道,在乙烯利喷液中加入1％醋酸钙,可抑制落果和落叶。 相似文献
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龙眼抽冬梢会恶化结果母枝的营养水平,影响其花芽分 化。生产中通过环割措施来控制,但在冬春气温变化异常的情 况下．环割的时机及切口宽度难于掌握。本试验探讨采用化学 (喷植物生长延缓剂)．结合物理(环割)手段,控制龙眼在 11-12月抽冬梢。 试验于2003年11月18日在洛江区河市镇梧宅村青年 果场进行。供试龙眼品种为“福眼”15年生树。试验设8个处 理:①比久1000x10-6;②40％乙烯利400x10-6;③15％多效唑 1000×10-6;④比久500x10-6+40％乙烯利300x10-6;⑤15％多效 唑500x10-6+40％乙烯利300x10-6;⑥比久500x10-6+15％多 效唑500x10-6;⑦环割;⑧清水对照(CK)。采用随机排列,单株 小区,3次重复,共24株。处理后定株、定梢观察冬梢抽发情 况．并于2004年3月14日随机调查各处理20个枝条的花芽 相似文献
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采用三因素五水平回归最优设计、砂培方法,研究了营养液中不同浓度 N、K、Ca 对椪柑实生苗生长与叶片叶绿素含量、光合强度及矿质养分吸收的影响,建立了相应的数学模型并对其进行了解析。结果表明;N 是影响柑桔叶片光合性状的重要因素,Ca、K 影响相对小些;叶片光合强度最大时的营养液组成是:N=230×10~(-6),K=130×10(-6),Ca=291×10~(-6);一定范围内,叶绿素含量、光合强度与叶片含 N、含 K 量成正相关;N 和 K 互相促进吸收,增施 K 时只有配合适量 N 才能更大程度地提高叶片的光合能力;叶片含 Ca 量随培养液与叶片中 N、K 含量升高而呈下降趋势。 相似文献
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破除葫芦种子休眠预措处理技术的研究 总被引：3，自引：0，他引：3
本试验通过破壳处理、光照处理、 0 .5 H2 O2 、 0 .0 5 MKNO3、 5 0× 1 0 - 6 GA3、 1 5× 1 0 - 6 6-BA、 2 0× 1 0 - 6 ABA、 5 0× 1 0 - 6 GA3+1 5× 1 0 - 6 6-BA、 5 0× 1 0 - 6 GA3+2 0× 1 0 - 6 ABA、 1 5× 1 0 - 6 6-BA+2 0× 1 0 - 6 ABA和 5 0× 1 0 - 6 GA3+1 5×1 0 - 6 6-BA+2 0× 1 0 - 6 ABA溶液浸种处理来探讨打破葫芦种子休眠的有效预处理方法。试验结果表明 0 .5 % H2 O2 浸种处理最有效 ,能将发芽率 6.8%的葫芦种子提高到 68% ,提高了 61 .2 %。本文对葫芦种子的休眠原因与机理进行了讨论。 相似文献
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蚯蚓粪基质辣椒穴盘苗播后喷施肥料效果的研究 总被引：9，自引：0，他引：9
以常规草炭系育苗基质及肥料配比和蚯蚓粪基质未肥料喷施为对照,设计3个肥料浓度水平(100×10-6、200×10-6和300×10-6N)和3个肥料喷施间隔次数(每次喷施肥料溶液、每隔1次灌水喷施1次肥料溶液和每隔2次灌水喷施1次肥料溶液),研究了播后肥料喷施对以蚯蚓粪为基质的辣椒穴盘苗生长发育的影响。结果表明:播后肥料喷施可以明显提高基质的EC值,显著增加辣椒叶片叶绿素a、b的含量,进而有效促进辣椒幼苗的生长发育。筛选出最佳播后喷施组合为200×10-6N每隔1次灌水喷施1次肥料溶液,其幼苗株高、茎粗、根体积、单株干重和壮苗指数比常规草炭系基质分别增加65.79%、37.93%、134.78%、222.23%和171.25%,差异均达显著水平。 相似文献
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龙眼是属于当年花芽分化当年开花结果类型的果树,而幼龄龙眼树,特别是初结果树,抽生初花穗都是长花穗(或称龙头穗)。主轴明显,二个侧花序间相隔距离大,花穗分枝级数多,花穗大花量多,花期早且长,雌花比例低,这些是幼龄龙眼树低产而不稳产的主要原因。一、材料和方法本试验在龙海市颜厝镇龙眼园进行。供试的龙眼树为4年生树,品种是“福眼”。试验树生长正常,树势旺盛,已开花1-2ａ,但未让其结果。试验于1997～1998年进行,供试株共40株,设四个处理。1、在花穗鱼只期(2月25日左右)喷150×10-6乙烯利+300×10-6多效唑,分化后期(4月20日～25日)将侧… 相似文献
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新纪世梨在我地区栽培已有近10年历史,每年成熟期裂果率常在15%以上。为解决裂果问题,于果实生长期的6月5日、6月15日、8月25日进行了如下9个喷雾处理:①GA 150×10-6(150ppm),②GA3100×10-5;③GA350× 10-5;④萘乙酸150×10-5;⑤萘乙酸100×10-6;⑥萘乙酸50×10-6,⑦0.5%ZnSO4⑧0.1%MCaCl2⑨清水(对照)。每处理1株,重复3次。 根据2年的试验看出,8个药剂处理对防止新世纪梨裂果均有作用,其中以0.5%ZnSO4为最佳,GA3150×10-5次之;从对果实含糖量测定看出,各处理与对照接近或较对照高;从果实外表看,除萘乙酸150×10-6和ChClsmo.lin使… 相似文献
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AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation. 相似文献
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AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration. 相似文献
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Zusammenfassung Die Leistungsprüfungen wurden im Zeitraum 1997 bis 2003 mit den Unterlagen Gisela 4 und 5, den Klonnummern 195/20 und 497/8 aus der Gisela-Serie sowie Weiroot 10, 13, 53, 72 und 158 durchgeführt. Dabei dienten Sämlinge von P1 (bulgarische Selektion aus Prunus mahaleb) als Kontrolle. Alle Unterlagen waren mit der Sorte Stella veredelt und im Dezember 1996 in der Versuchsanlage der Agraruniversität in Plovdiv, Bulgarien, im Abstand von 6 m×4,5 m gepflanzt worden. Dabei erfolgte ein Pflanzschnitt. Nach Abschluss der natürlichen Kronenentwicklung wurde jedes Jahr ein Winterschnitt vorgenommen. Der Boden wurde durch mechanische Bearbeitung offen gehalten und nach dem 4. Standjahr wurden die Baumstreifen mit Herbiziden behandelt. Die Wasserversorgung erfolgte durch eine dem natürlichen Gefälle folgende Überflutung, allerdings nicht immer zum optimalen Zeitpunkt, da keine eigene Wasserquelle zur Verfügung stand.Basierend auf den Ergebnissen bis zum Anfang des 7. Standjahres können die untersuchten Unterlagen in zwei Gruppen differenziert werden: starkwüchsig—Weiroot 10, P1 und Weiroot 13; mittelstarkwachsend bis schwachwüchsig—Gi 497/8, Gisela 4, Weiroot 53, Weiroot 158, Gi 195/20, Weiroot 72 und Gisela 5. Letztere zeichnete sich durch besondere Schwachwüchsigkeit aus. Die meisten Wurzelschosser bildeten Gisela 4, Weiroot 10 und Weiroot 13. Weiroot 53, Weiroot 72 und Weiroot 158 entwickelten deutlich weniger und P1, Gisela 5, Gi 195/20 sowie Gi 497/8 keine Wurzelschosser. Den frühesten Blühbeginn induzierte Gisela 4. Die anderen Unterlagen führten, in Abhängigkeit von den Temperaturbedingungen des jeweiligen Jahres, zu einer Verspätung der Blüte: P1 und Weiroot 10 um 1–2 Tage; Gi 497/8, Weiroot 13 und Weiroot 158 um 2–4 Tage; Weiroot 72 um 2–7 Tage; Gi 195/20 um 3–6 Tage; Weiroot 53 um 3–8 Tage und Gisela 5 um 3–10 Tage. Die Reifezeit der Früchte war bei den Bäumen auf Gisela 5 im Vergleich zu den anderen Varianten um 2–3 Tage verspätet. Gisela 5, Weiroot 72 und Gisela 4 induzierten bei der aufveredelten Sorte die höchsten Ertragsleistungen, P1 die geringsten. Bei den Bäumen auf Gisela 5 war die Fruchtgröße geringer als bei den anderen Unterlagen. Bäume auf Gisela 5 brauchen intensive Pflege. Nur wenn alle Produktionsfaktoren und kulturtechnischen Maßnahmen optimiert werden, kann das hohe Ertragspotenzial dieser Unterlage ausgeschöpft werden. 相似文献
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《International Journal of Fruit Science》2013,13(3):101-120
Abstract Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared. 相似文献
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多效唑对猕猴桃离体试管苗生长及内源激素的影响 总被引：18，自引：0，他引：18
多效唑（ＰＰ３３３）处理猕猴桃试管苗，降低了其生长强度；植株体内的ＧＡ３、ＩＡＡ和ＺＴ含量下降，ＡＢＡ的含量上升，乙烯释放率增加；并且能降低外源的ＧＡ３和ＩＡＡ促进生长的作用，而外源的ＧＡ３和ＩＡＡ又能不同程度地逆转多效唑的抑制作用，使植株恢复生长。 相似文献
16.
HU Zhi-cheng ZHU Jia-yuan ZHU Bin GUO Dong CHEN Bin ZHANG Kai HU Kun-hua LI Ming-tao TANG Bing 《园艺学报》2011,27(9):1802-1806
AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars. 相似文献
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The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species. 相似文献
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AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation. 相似文献
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Duncan Brean W. Boyle Shannon Breininger David R. Schmalzer Paul A. 《Landscape Ecology》1999,14(3):291-309
Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere. 相似文献
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AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis. 相似文献