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根据番茄斑萎病毒属(Tospovirus)中6种病毒S RNA上的N基因序列设计特异性引物,建立可同时检测这6种Tospovirus病毒的多重PCR体系。多重PCR扩增结果显示,番茄环纹斑点病毒(776 bp)、甜瓜黄斑病毒(505 bp)、鸢尾黄斑病毒(296 bp)、凤仙花坏死斑病毒(221 bp)、番茄斑萎病毒(175 bp)和番茄褪绿斑病毒(110 bp)均出现清晰的目标条带,各病毒的特异性引物不会对其他病毒产生非特异性扩增。本研究建立的6种Tospovirus病毒的多重PCR检测方法,有助于提高目标病毒的检测效率。 相似文献
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广东兰花病毒病调查和病原检测 总被引：7，自引：0，他引：7
本试验主要是采用症状观察和ELISA方法榆测兰仡病毒。检测材料是从广州兰圃、芳村兰场、华南植物园、深圳苗圃场采集的兰花标本。兰花病株症状类型有花叶、黑褐色坏死斑或斑块：有的叶片在褪绿处变薄，后变灰、干缩、破裂成丝。ELISA检测结果表明墨兰、文心兰、大花惠兰(组培苗)、蝴蝶兰、万代兰和卡特兰等几个品种共22个样本含有病毒，其中含有建兰花叶病毒(CyMV)的有10个品种，含有齿兰环斑病毒(ORSV)的有7个品种，民时含有两种以上病毒的有3个品种6个样本。 相似文献
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辣椒是云南省主要经济作物之一,近年来病毒病尤其是正番茄斑萎病毒属病毒发病严重,影响了辣椒产量和品质。利用RT-PCR技术对从云南辣椒主产区采集的疑似感染正番茄斑萎病毒属病毒的25份辣椒样品进行分子鉴定,结果显示,12份样品检测出正番茄斑萎病毒属病毒,检出率为48.0%,其中6份是番茄斑萎病毒tomato spotted wilt orthotospovirus (TSWV),检出率为24.0%;5份是番茄环纹斑点病毒tomato zonate spot orthotospovirus (TZSV),检出率为20.0%;有1份是TSWV和TZSV复合侵染,检出率为4.0%,这是在云南辣椒生产上首次发现TSWV和TZSV的复合侵染。通过鉴定,初步了解正番茄斑萎病毒属病毒在云南辣椒生产中的发生情况和种类,为制定云南地区该属病毒的防治策略提供理论依据。 相似文献
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番茄疮痂病的发生和防治 总被引：1，自引：0，他引：1
番茄细菌性疮痂病又称斑点病,分布于全世界６０多个国家,９０年代初在我国不断蔓延,现已成为番茄上一个重要病害。近二年,该病在偃师市危害严重,影响生产很大。一、症状该病主要危害番茄叶片及果实,近地面老叶先发病,逐渐向上部叶片发展,发病初期在叶背面形成水渍状暗绿色小斑,逐渐扩展成圆形或连接成不规则黄色病斑,病斑表面粗糙不平,周围有黄色晕圈,后期叶片干枯质脆。茎部感病先在茎沟处出现褪绿色水渍状小斑点,扩展后形成长椭圆形黑褐色病斑,裂开后呈疮痂状。果实上主要危害着色前的幼果和青果,果面先出现褪绿色斑点,后… 相似文献
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《湖北植保》2002,(1)
为害作物 :除西葫芦外 ,还为害南瓜、笋瓜、甜瓜、西瓜、冬瓜等瓜类蔬菜。为害症状 :植株上部叶片沿叶脉失绿 ,并出现黄绿斑点 ,渐渐全株黄化 ,叶片皱缩向下卷曲 ,节间短 ,植株矮化。枯死株后期花冠扭曲畸形 ,大部不能结瓜或瓜小而畸形。或苗期 4～ 5片叶时开始发病 ,新叶表现明脉 ,有褪色斑点 ,继而花叶 ,有深绿色疱斑 ,重病株顶叶畸形鸡爪状 ,病株矮化 ,不结瓜或瓜表面有环状斑或绿色斑驳 ,皱缩、畸形。分类属性 :黄瓜花叶病毒 ( CMV)、甜瓜花叶病毒 ( MMV)、西瓜花叶病毒 ( WMV)等多种病毒分布区域 :全国发病特点 :高温干旱有利于有… 相似文献
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广西烟草病毒病发生情况调查和病原病毒的鉴定 总被引：1，自引：0，他引：1
2010~2011年对广西百色市、河池市和贺州市的主要烤烟产区进行烟草病毒病发生情况和种类调查。结果显示,广西烟草病毒病以由烟草花叶病毒和黄瓜花叶病毒引起的花叶类型症状为主,一般发病率1.0%~10.0%,个别严重发病田块发病率达到82.0%~100.0%;马铃薯Y病毒病、番茄斑萎病毒病和曲叶病则为局部发生病害,其中马铃薯Y病毒病在靖西县、南丹县、富川县和钟山县零星发生,发病率0.1%~5.0%,个别田块达到20.0%;番茄斑萎病毒病主要在靖西县、隆林县、田林县和南丹县发生;曲叶病仅在靖西县、隆林县、田林县和乐业县等百色市烟区发现。采用间接ELISA、PCR和RT-PCR等方法对田间采集的185个各类疑似病毒病样进行病毒种类检测,其中145个样品检测出病毒,分别是烟草花叶病毒、黄瓜花叶病毒、马铃薯Y病毒、番茄环纹斑点病毒、中国番茄曲叶病毒和中国番茄黄化曲叶病毒,以及分别由上述2种病毒与云南辣椒曲叶病毒重组而来的2种重组病毒,其中以TMV的检出率最高,占检出样品的86.7%,是广西烟草病毒病的优势病原病毒。 相似文献
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Elliot W KITAJIMA Hideki KONDO Anne MACKENZIE Jorge Alberto M REZENDE Ricardo GIORIA Adrian GIBBS Tetsuo TAMADA 《Journal of General Plant Pathology》2001,67(3):231-237
Cytopathic effects in orchid leaf tissues infected with Australian, Japanese and Brazilian isolates of Orchid fleck virus
(OFV) were indistinguishable and like those previously described in the literature. Cells had an electron-lucent viroplasm
with unenveloped rod-shaped virions in the nucleus and cytoplasm, often associated with the inner membrane of the nuclear
envelope and the endoplasmic reticulum. Antiserum raised against a Japanese isolate of OFV reacted with Brazilian and Australian
isolates in ELISA, and when used for immuno-gold labelling, also reacted in situ with the rod-shaped virions and the intranuclear viroplasm of all three isolates. These results suggest that the viroplasm
is where structural proteins accumulate and virions are formed.
Received 13 December 2000/ Accepted in revised form 13 April 2001 相似文献
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K. S. Kubo J. Freitas-Astúa M. A. Machado E. W. Kitajima 《Journal of General Plant Pathology》2009,75(3):250-255
Brevipalpus-transmitted viruses (BTV) cause chlorotic, necrotic and/or ringspot lesions in leaves and stems of orchids, citrus, coffee
and several other plant species. There are two different types of BTVs, the nuclear and the cytoplasmic, based on maturation
locale in the cell and particle morphology. The orchid fleck virus (OFV) is a BTV that infects orchids. Its short rodlike
particles are 32–40 nm in diameter, 100–150 nm in length. OFV is found in the nucleus and is associated with intranuclear
electronlucent viroplasms. In 1999, transmission electron microscopy analysis revealed a distinct type of virus causing orchid
fleck symptoms. The bacilliform particles, 70–80 nm in diameter and 110–120 nm in length, induced electron-dense viroplasm
inclusions in infected cells and resembled the cytoplasmic type associated with BTV, such as the citrus leprosis virus C.
Our objective in the present study was to verify whether the cytoplasmic type virus found in orchids could be amplified using
primers for other cytoplasmic BTVs, such as CiLV-C and Solanum
violaefolium ringspot virus (SvRSV). Additionally, we aimed to differentiate the two BTVs found in orchids: the nuclear and the cytoplasmic
types of OFV using microscopy and molecular and serological tools. This virus was not amplified by the CiLV-C and SvRSV primers,
and neither the molecular nor the serological tools available to the OFV diagnosis reacted with it, demonstrating that they
are definitely different viruses. 相似文献
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A tissue-print hybridization assay for the detection and localization of viruses in orchids is described. Orchid leaves were cut transversely and the cut surfaces were printed on to nitrocellulose paper. Because orchid leaves are often succulent, there is excellent transfer of plant sap to the membrane, resulting in clear tissue prints. The nucleic acid on the nitrocellulose membrane was then hybridized to radio-labelled cDNAs of cymbidium mosaic virus (CyMV) and odontoglossum ringspot virus (ORSV), and the viruses were detected by autoradiography. The assay was able to detect as little as 20 pg of purified virus, which is 2–3 orders of magnitude more sensitive than the enzyme-linked immunosorbent assay (ELISA) for CyMV. When tissue prints derived from different parts of the plants were used for hybridization, the method was able to show the distribution of viruses within the plants. On the basis of its sensitivity and ease of use, this method should find wide application in the detection and localization of viruses in orchids as well as in other succulent plants. 相似文献
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Grapevines of the Maltese islands were surveyed for the presence of virus diseases. Symptoms of fanleaf degeneration, leafroll, rugose wood and fleck were observed in the main grape-growing areas. The occurrence of these diseases was experimentally confirmed by graft transmission to woody indicators, which also revealed the presence of vein necrosis, a latent virus-like disorder. Viruses identified were grapevine fanleaf nepovirus, grapevine fleck virus, grapevine A closterovirus, grapevine leafroll-associated closteroviruses I and III. ELISA tests showed that 86% of 322 vines individually checked were infected by one or more viruses. 相似文献
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Grapevines were surveyed for the presence of virus and virus-like diseases in the Albanian viticultural districts of Shkoder, Lesh, Kruje, Durres, Tirana, Elbasan, Lushnje and Vlora. Symptoms of grapevine degeneration, leafroll and rugose wood were observed in all areas surveyed, whereas fleck was found in volunteer plants of Vitis rupestris at Elbasan, and enation disease in a few vines near Durres. Viruses identified were grapevine fanleaf nepovirus, grapevine fleck virus, grapevine virus A, and grapevine leafroll-associated closteroviruses I and III. ELISA tests showed that 83.5% of 530 Vitis vinifera vines and 46% of 24 American rootstocks individually checked were infected by one or more viruses. The presence of Xiphinema index , the major vector of grapevine fanleaf nepovirus, was recorded from vineyards affected by yellow mosaic. 相似文献
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Celery infected with celery mosaic virus or parsnip yellow fleck virus contained the furanocoumarins angelicin, bergapten, psoralen, trimethylpsoralen and xanthotoxin in concentrations from 0.3 to 3.6 μg/g fresh mass. They were not detected in celery plants grown in aseptic culture, and only occasionally found in apparently healthy plants in the glasshouse. 相似文献
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Characterization of the first two viruses described from wild populations of hammer orchids (Drakaea spp.) in Australia 
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下载免费PDF全文 J. W. L. Ong R. D. Phillips K. W. Dixon M. G. K. Jones S. J. Wylie 《Plant pathology》2016,65(1):163-172
Sequences representing the genomes of two distinct virus isolates infecting wild plants of two members of the genus Drakaea (hammer orchids) in Western Australia are described. The virus isolated from Drakaea livida has a bipartite genome of 4490 nt (RNA1) and 2905 nt (RNA2) that shares closest sequence and structural similarity to members of the genus Pecluvirus, family Virgaviridae, described from legumes in the Indian subcontinent and West Africa. However, it differs from pecluviruses by lacking a P39 protein on RNA2 and having a cysteine‐rich protein gene located 3′ of the triple gene block protein genes. It is the first peclu‐like virus to be described from Australia. The name Drakaea virus A is proposed (DVA; proposed member of the family Virgaviridae, genus unassigned). The second virus isolate was identified from Drakaea elastica, a species classed as endangered under conservation legislation. The genome sequence of this virus shares closest identity with isolates of Donkey orchid symptomless virus (DOSV; proposed member of the order Tymovirales, family and genus unassigned), a species described previously from wild Caladenia and Diuris orchids in the same region. These viruses are the first to be isolated from wild Drakaea populations and are proposed to have an ancient association with their orchid hosts. 相似文献
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Tatsuya Nagata Luisa S. Dutra Priscila A. Oliveira Antonio C. de Ávila Alice K. Inoue-Nagata 《Journal of General Plant Pathology》2010,76(4):268-272
A novel viral disease of melon, caused by Melon yellowing-associated virus (MYaV), has become one of the most serious problems
for melon production in Brazil. Despite its importance, little is known about the genome sequence of the virus. In this study,
the triple gene block genomic region including the 3′-end region of an isolate of MYaV was elucidated (ca. 3.1 kb). Sweet
potato chlorotic fleck virus, a newly suggested carlavirus species, was the most closely related virus with 62% nucleotide
identity. The presence of two heptanucleotide-motifs for subgenomic RNA synthesis was confirmed in this sequence. 相似文献