共查询到19条相似文献，搜索用时 203 毫秒
1.
为揭示脂肪分化相关蛋白(adipose differentiation-related protein,ADFP)变化对家禽脂肪性状形成的作用,实验采用SYBRGreenΙ荧光定量法,首次测定了四川山地乌骨鸡1、14、28、42、56、70d和84d胸肌、肝脏、腹脂和皮脂组织中ADFP基因相对表达量。结果表明:ADFP基因表达量在28d,腹脂和肝脏中的表达量显著高于胸肌(P<0.05),且腹脂>肝脏>皮脂>胸肌;在42d,胸肌中的表达量显著高于皮脂(P<0.05),且胸肌>肝脏>腹脂>皮脂;在84d时,胸肌中的表达量显著高于肝脏中的(P<0.05),且胸肌>腹脂>肝脏。腹脂中ADFP基因的相对表达量的变化与活重和冠重性状的变化显著相关(P<0.05),胸肌中ADFP基因的相对表达量发育性的变化与肌内脂肪含量的变化显著相关(P<0.05)。ADFP基因可能在鸡脂肪性状形成的过程中发挥重要的调控作用。 相似文献
2.
《中国家禽》2016,(24)
采用荧光定量PCR技术,联合分析了不同日龄(28、49、70、91和112日龄)武定鸡载脂蛋白B基因(APOB)、脂肪酸转运蛋白1基因(FATP1)和脂肪分化相关蛋白基因(ADFP)在皮脂、腹脂、肝脏、胸肌等组织中的表达量变化,及其与活重、皮脂厚、冠重等脂肪性状形成的相关性。结果显示,ADFP基因在腹脂49日龄时的表达量显著低于28、70、91和112日龄(P0.05),及该基因在112日龄时胸肌中的表达量显著高于皮脂、腹脂和肝脏(P0.05);FATP1基因在28日龄时胸肌中的相对表达量显著高于皮脂、腹脂和肝脏(P0.05);APOB基因在肝脏49日龄时的表达量显著高于28、70和91日龄(P0.05),并且该基因在49、70、91和112日龄时肝脏中的表达量显著高于皮脂、腹脂和胸肌(P0.05)。ADFP基因和APOB基因在皮脂中的表达存在显著相关(P0.05),ADFP基因和FATP1基因在肝脏中的表达存在显著相关(P0.05),推断这三个脂肪沉积相关基因可能在武定鸡脂肪性状的形成过程中具有协同调控作用。 相似文献
3.
《中国畜牧杂志》2017,(9)
为揭示脂肪分化相关蛋白基因(ADFP)、脂肪酸转运蛋白1基因(FATP1)和载脂蛋白B基因(Apo B)表达量变化对家禽脂肪沉积的影响,实验采用SYBR GreenΙ荧光定量法,首次测定了盐津乌骨鸡28、49、70、91、112 d皮脂、腹脂、肝脏和胸肌组织中ADFP、FATP1和Apo B基因的相对表达量。结果表明:ADFP基因在28 d各组织中表达量为肌肉腹脂皮脂肝脏,且胸肌中表达量显著高于其他3种组织(P0.05),在其他4个时间点各组织间表达量差异不显著(P0.05);FATP1基因在49 d各组织中表达量为胸肌腹脂肝脏皮脂,且胸肌中表达量显著高于皮脂(P0.05),在其他4个时间点各组织间表达量差异不显著(P0.05);Apo B基因在28、49、70、91、112 d 5个时间点各组织间表达量差异不显著(P0.05),但在肝脏中的表达量高于其他组织。3个基因表达量变化可能在脂肪沉积过程中发挥调控作用。 相似文献
4.
兴义矮脚鸡屠宰性能、肌肉品质及LPL基因表达的研究 总被引：3，自引：1，他引：3
为了研究兴义矮脚鸡的屠宰性能、肌肉品质、肌肉氨基酸含量及LPL基因mRNA的表达情况。屠宰了14周龄同批孵出的兴义矮脚鸡100只(公、母各半)。结果表明:公、母鸡屠宰率分别为87.40%、87.97%,公鸡的活重、半净膛率、全净膛率等均极显著(P<0.01)地高于母鸡;腹脂率极显著(P<0.01)低于母鸡。公鸡肌肉的脂肪含量(1.99%)、干物质含量(26.24%)显著低于母鸡的2.37%、27.31%(P<0.05),公鸡的蛋氨酸、缬氨酸含量显著低于母鸡(P<0.05)。腹脂、皮脂、肌肉的LPL mRNA表达量有性别差异性,而且也存在肝、腹脂、皮脂、肌肉等组织差异性。综上所述,兴义矮脚鸡公鸡产肉性能较好;母鸡的风味和营养价值较高,LPL基因mRNA的表达有组织和性别差异性。 相似文献
5.
《养猪》2016,(5)
为研究大河乌猪采食特性与生长性能的相关性,选择云南富源县大河种猪场饲养的日龄相近、体重30 kg左右的50头大河乌猪为试验对象,随机分为5组,每组10头,每头均打上电子识别耳标,进入测定舍测定,试验猪100 kg体重结束试验。试验期间饲喂相同饲粮,自由采食和饮水。结果显示:1)30~50 kg与50~100 kg体重阶段日采食量差异不显著,总耗料量、日增重及料重比均差异显著(P0.05)。2)双变量分析表明,30~50 kg体重阶段大河乌猪的日采食量与日增重、料重比呈强度相关(r=0.960;r=0.932);总耗料量与日增重、料重比呈强度相关(r=0.988;r=0.861);日增重与料重比呈强度相关(r=0.845)。50~100 kg体重阶段大河乌猪的日采食量与日增重、料重比呈强度相关(r=0.981;r=0.990);总耗料量与日增重、料重比呈强度相关(r=0.978;r=0.984);日增重与料重比呈强度相关(r=0.969)。3)30~50 kg和50~100 kg体重阶段日采食量、总耗料量、日增重与料重比之间均呈极显著正相关(P0.01)。 相似文献
6.
试验采用2×2因子设计(日粮类型0%或1%CLA,肉鸡性别)研究日粮CLA对不同性别肉仔鸡生长性能、屠体性状和脂肪代谢相关酶的影响。结果表明:日粮中添加1%CLA可以显著增加各性别肉仔鸡各阶段的体增重,并改善了4～6、0～6周的饲料转化效率(P<0.05),可以显著提高各性别肉仔鸡的胸肌率、同时显著降低各性别肉仔鸡的腹脂率(P<0.05),显著降低了各性别肉仔鸡腹脂脂蛋白脂酶(LPL)活性及其mRNA相对表达量(P<0.05),显著降低了各性别肉仔鸡腹脂及肝脏硬脂酰辅酶A去饱和酶(SCD)mRNA相对表达量(P<0.05)。另外除体增重部分指标外,性别和日粮CLA对肉仔鸡屠体性状和脂肪代谢相关酶的影响不存在互作关系。 相似文献
7.
丙氨酰-谷氨酰胺对哲罗鱼仔鱼生长和抗氧化能力的影响 总被引：2，自引：1，他引：1
本试验旨在研究饲喂丙氨酰-谷氨酰胺(Ala-Gin)对哲罗鱼(Hucho taimen)仔鱼生长和抗氧化能力的影响.试验分6个组,每组3个重复,每重复1 000尾鱼.各组饵料分别在基础饵料基础上添加0(对照组)、0.125%、0.250%、0.500%、0.750%和1.000%的Ala-Gin.试验共进行8周.结果表明:与对照组相比,0.125%组对哲罗鱼仔鱼的生长未产生显著影响(P>0.05);0.500%～1.000%组显著(P<0.05)或极显著(P<0.01)提高增重率和特定生长率;当添加量大于0.125%时,成活率显著(P<0.05)或极显著(P<0.01)提高;当添加量大于0.500%时,鱼体组织匀浆液中谷氨酰胺(Gln)含量显著升高(P<0.05);0.750%和1.000%组鱼体组织匀浆液中谷氨酸(Glu)含量显著提高(P<0.05);0.500%和0.750%组Na+,K+-ATPase活性显著升高(P<0.05);各添加组SOD活性显著提高(P<0.05),MDA含量显著(P<0.05)或极显著降低(P<0.01);氨基酸含量无显著变化(P>0.05).由此可知,添加Ala-Gin可提高哲罗鱼仔鱼的生长性能和抗氧化能力,适宜的添加水平为0.750%. 相似文献
8.
9.
为探究添加不同形态氮素对紫花苜蓿幼根中硝酸盐转运蛋白基因NRT1.3表达的影响,试验设置4个处理,分别为对照(不加氮)、添加硝态氮、添加铵态氮和添加混合态氮,在紫花苜蓿分枝期测定了添加不同形态氮素后紫花苜蓿幼根中硝酸盐转运蛋白基因NRT1.3表达量的差异以及苜蓿生理指标、土壤氮含量对该基因表达的影响。结果表明:紫花苜蓿根中硝酸盐转运蛋白基因NRT1.3的表达量随着氮素的添加而显著增加(P0.05),在添加混合态氮的处理中达到最高;土壤全氮、硝态氮和铵态氮浓度随氮素添加而显著升高(P0.05),紫花苜蓿根中硝酸盐转运蛋白基因NRT1.3的表达量与土壤氮素浓度呈显著正相关关系(P0.05)。此外,试验结果也表明NRT1.3基因的表达与紫花苜蓿的株高、鲜重及叶绿素、硝酸盐含量也呈显著正相关关系(P0.05)。 相似文献
10.
紫花苜蓿对猪骨骼肌细胞肝X受体基因和蛋白表达的影响 总被引：1，自引：0，他引：1
为了探讨紫花苜蓿对猪骨骼肌细胞中肝X受体(liver X receptors,LXRs)表达的影响及其与胴体肉质的关系,采用免疫组织化学方法检测LXRs蛋白的分布,通过酶联免疫方法(enzyme-linked immunosorbent assay,ELISA)研究LXRs蛋白总量的改变,以荧光定量PCR方法分析猪骨骼肌细胞中LXRs基因mRNA的表达变化。结果表明,LXRs定位于骨骼肌细胞的细胞质、细胞膜和细胞核内;与对照组相比,日粮中添加4%紫花苜蓿能使LXRs基因和蛋白表达量明显上升(P<0.05),并使猪的瘦肉率降低(P>0.05),肌内脂肪含量显著增高(P<0.05),同时大理石纹评分较好(P<0.05),滴水损失下降(P<0.05),细胞间隙变小(P<0.05);添加6%紫花苜蓿使LXRs基因和蛋白表达量上升(P<0.05),但低于4%紫花苜蓿组(P>0.05),同时大理石纹评分及失水率增高(P<0.05),板油重下降(P<0.05)。结果提示,紫花苜蓿添加量的不同对猪肉品质的作用效果不同,为紫花苜蓿在养猪业的科学应用奠定了理论基础。 相似文献
11.
Nkrumah JD Keisler DH Crews DH Basarab JA Wang Z Li C Price MA Okine EK Moore SS 《Journal of animal science》2007,85(9):2147-2155
Leptin is the hormone product of the obese gene that is synthesized and predominantly expressed by adipocytes. This study estimated the genetic variation in serum leptin concentration and evaluated the genetic and phenotypic relationships of serum leptin concentration with performance, efficiency of gain, and carcass merit. There were 464 steers with records for serum leptin concentration, performance, and efficiency of gain and 381 steers with records for carcass traits. The analyses included a total of 813 steers, including those without phenotypic records. Phenotypic and genetic parameter estimates were obtained using SAS and ASREML, respectively. Serum leptin concentration was moderately heritable (h2 = 0.34 +/- 0.13) and averaged 13.91 (SD = 5.74) ng/mL. Sire breed differences in serum leptin concentration correlated well with breed differences in body composition. Specifically, the serum leptin concentration was 20% greater in Angus-sired steers compared with Charolais-sired steers (P < 0.001). Consequently, ultrasound backfat (27%), carcass 12th-rib fat (31%), ultrasound marbling (14%), and carcass marbling (15%) were less in Charolais- than Angus-sired steers (P < 0.001). Conversely, carcass LM area (P = 0.05) and carcass lean meat yield (P < 0.001) were greater in Charolais- compared with Angus-sired steers. Steers with greater serum leptin concentration also had greater DMI (P < 0.001), greater residual feed intake (P = 0.04), and partial efficiency of growth (P = 0.01), but did not differ in feed conversion ratio (P > 0.10). Serum leptin concentration was correlated phenotypically with ultrasound backfat (r = 0.41; P < 0.001), carcass 12th-rib fat (r = 0.42; P < 0.001), ultrasound marbling (r = 0.25; P < 0.01), carcass marbling (r = 0.28; P < 0.01), ultrasound LM area (r = -0.19; P < 0.01), carcass LM area (r = -0.17; P < 0.05), lean meat yield (r = -0.38; P < 0.001), and yield grade (r = 0.32; P < 0.001). The corresponding genetic correlations were generally greater than the phenotypic correlations and included ultrasound backfat (r = 0.76 +/- 0.19), carcass 12th-rib fat (r = 0.54 +/- 0.23), ultrasound marbling (r = 0.27 +/- 0.22), carcass marbling (r = 0.76 +/- 0.21), ultrasound LM area (r = -0.71 +/- 0.19), carcass LM area (r = -0.75 +/- 0.20), lean meat yield (r = -0.59 +/- 0.22), and yield grade (r = 0.39 +/- 0.26). Serum leptin concentration can be a valuable tool that can be incorporated into appropriate selection programs to favorably improve the carcass merit of cattle. 相似文献
12.
Hoenig M McGoldrick JB deBeer M Demacker PN Ferguson DC 《Domestic animal endocrinology》2006,30(4):333-344
Post-heparin plasma activity of lipoprotein lipase (LPL) and hepatic lipase (HL), and fat and muscle activity of LPL were measured in neutered lean and obese cats. Lipoprotein lipase, hormone-sensitive lipase (HSL), and tumor necrosis factor a (TNF) mRNA were measured in muscle and fat tissue with real-time PCR using primers for feline LPL, HSL, and TNF. Lipoprotein lipase plasma and fat activity and fat mRNA levels were significantly lower (50, 80, and 50%, respectively) in obese cats than lean cats, whereas the muscle/fat ratio of LPL was significantly higher in obese compared to lean cats. The activity of HL was not different between the groups. Hormone-sensitive lipase mRNA levels were significantly higher in obese than lean cats. The level of fat TNF also was significantly higher in obese cats than in lean cats, whereas the level in muscle was not different. The lower LPL activity and mRNA expression in fat and the higher LPL and HSL mRNA expression in muscle in obese cats compared to lean cats expectedly favor a redistribution of fatty acids from fat to muscle tissue where they can be deposited or used for energy in times of need. Tumor necrosis factor alpha may regulate this repartitioning process through suppression of adipocyte LPL. 相似文献
13.
苜蓿皂苷对断奶仔猪生长性能、肠道菌群、组织抗氧化能力及相关酶mRNA表达的影响 总被引：1，自引：0，他引：1
本试验旨在研究苜蓿皂苷对断奶仔猪生长性能、肠道菌群、组织抗氧化能力及相关酶mRNA表达的影响。选取24头平均体重为8 kg的大×长杂交断奶仔猪,随机分为2组,每组3个重复,每个重复4头猪。对照组饲喂基础饲粮,苜蓿皂苷组饲喂在基础饲粮中添加0.25%苜蓿皂苷的饲粮。预试期10 d,正试期30 d。结果表明:1)与对照组相比,饲粮中添加苜蓿皂苷可显著提高断奶仔猪平均日增重(P0.05),并显著降低料重比(P0.05)。2)与对照组相比,饲粮中添加苜蓿皂苷可显著降低仔猪十二指肠和盲肠的pH(P0.05),显著提高十二指肠、空肠和回肠中乳酸菌数量(P0.05)。3)与对照组相比,饲粮中添加苜蓿皂苷可显著提高仔猪肝脏和肾脏中谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性(P0.05),并显著提高仔猪肝脏和空肠中GSH-Px mRNA表达量及十二指肠和回肠中CAT mRNA表达量(P0.05)。综上,苜蓿皂苷可以提高断奶仔猪生长性能,增强其组织抗氧化能力并有效改善其肠道菌群。 相似文献
14.
维生素A水平对生长猪生产性能、肝脏维生素A含量和血清免疫参数的影响 总被引：4，自引：0，他引：4
试验研究饲粮添加不同水平维生素A(VA) (0、1300、2600、5000、10000、15000IU/kg)对22 -50kg生长猪生产性能、胴体品质、胴体无脂瘦肉增重、肝脏和血浆中VA含量、血清免疫参数的影响。结果表明 ,添加VA1300 -10000IU/kg 提高了日增重 (P>0.05)、极显著地降低料重比 (P<0.01) ;但VA添加水平从1300IU/kg增加至10000IU/kg,料重比显著提高 (P<0.05) ,采食量也极显著提高 (P<0.01)。各组屠宰率、瘦肉率、脂肪率、背膘厚、胴体瘦肉成分都无显著差异 (P>0.05) ,眼肌面积则随VA水平的升高而趋于增大(P>0.05)。胴体瘦肉和无脂瘦肉日增重在添加VA1300IU/kg 和10000IU/kg两组间很接近 ,而且优于其它组 (P<0.05)。随着VA水平的提高 ,肝脏中VA含量极显著提高 (P<0.01) ,至添加VA10000IU/kg组最高 ,之后略下降 ;添加VA有使血浆中VA含量升高的趋势 (P>0.05)。各组血清中IgG、IgA、IgM含量都无显著差异 (P>0.05) ,添加VA使T淋巴细胞中CD3+ 和CD8+ 亚群比例趋于升高 (P>0.05),随着添加VA水平提高 ,血清T淋巴细胞中CD4+/CD8+比值趋于下降 ,至添加VA10000IU/kg 组最低 ,在VA15000IU/kg 组又明显上升 (P>0.05)。以肝脏中VA含量为指标 ,生长猪VA适宜水平为在基础饲料上添加10000IU/kg ,但根据生产性能等其它指标 ,添加1300IU 相似文献
15.
Comparing mRNA levels of genes encoding leptin,leptin receptor,and lipoprotein lipase between dairy and beef cattle 总被引：3，自引：0，他引：3
Ren MQ Wegner J Bellmann O Brockmann GA Schneider F Teuscher F Ender K 《Domestic animal endocrinology》2002,23(3):371-381
Body weight and fat mass vary distinctly between German Holstein (dairy cattle) and Charolais (beef cattle). The aim of this study was to determine whether the expression of the obese (Ob) gene and lipoprotein lipase (LPL) gene in fat tissues and expression of the long isoform leptin receptor (Ob-Rb) gene in the hypothalamus were different between these two cattle breeds. Body weight and the area of longissimus muscle cross-section of German Holstein were lower (P<0.001), while body fat content, as well as the omental and perirenal fat mass were higher (P<0.001), compared to Charolais. Plasma insulin and leptin levels between two cattle breeds were determined by radioimmunoassay. Compared to Charolais, plasma insulin concentrations were significantly higher (P<0.01), and plasma leptin levels were tended to be higher (P<0.1) in German Holstein. Ob mRNA levels in subcutaneous and perirenal fat depots, but not in the omental fat depot, were significantly higher (P<0.05) in German Holstein than in Charolais. LPL mRNA expression in the perirenal fat depot of German Holstein was greater in abundance than that of Charolais. No significantly different LPL mRNA levels were found in subcutaneous and omental fat depots, and Ob-Rb mRNA levels in the hypothalamus between these two cattle breeds (P<0.05). Both Ob and LPL expression was greater in perirenal and omental fat depots than in the subcutaneous fat depot (P<0.05). Data indicated that in bovine the Ob and LPL gene expression levels in perirenal fats are an important index that is associated with body fat content, while Ob-Rb in hypothalamus is not. 相似文献
16.
Expression of adiponectin and its receptors in swine 总被引：1，自引：0，他引：1
Adiponectin is an adipocyte-derived hormone that plays an important role in lipid metabolism and glucose homeostasis. Objectives of this study were 1) to determine the presence and distribution of adiponectin and its receptors 1 and 2 (adipoR1 and adipoR2) in porcine tissues; 2) to characterize pig adiponectin, adipoR1, and adipoR2 mRNA levels in various fat depots from three different breeds of pigs; and 3) to study, in stromal-vascular cell culture, the effects of leptin and tumor necrosis factor-alpha (TNFalpha) on pig adiponectin, adipoR1, and adipoR2 gene expression. To this end, fat Chinese Upton Meishan (UM, n = 10), lean Ham Line (HL, n = 10), and Large White (LW, n = 10) gilts were used. We report the isolation of partial cDNA sequences of pig adipoR1 and adipoR2. Porcine-deduced AA sequences share 97 to 100% homology with human and murine sequences. Pig adipoR1 mRNA is abundant in skeletal muscle, visceral fat, and s.c. fat tissues, whereas adipoR2 mRNA is predominantly expressed in liver, heart, skeletal muscle, and visceral and s.c. fat tissues. Pig adiponectin mRNA levels in s.c. and visceral fat tissues were not associated with plasma insulin and glucose in fasting animals. Subcutaneous (r = -0.44, P < 0.05), visceral (r = -0.43, P < 0.05), and total body fat (r = -0.42, P < 0.05) weights were negatively correlated with adiponectin mRNA levels measured in visceral, but not s.c., fat. Pig adipoR1 and adipoR2 mRNA levels, in visceral fat, were less expressed in fat UM gilts than in the lean HL gilts (P < 0.05). Inverse associations were found between s.c. (r = -0.57, P < 0.01), visceral (r = -0.46, P < 0.05), and total body fat (r = -0.56, P < 0.01) weights and adipoR2 mRNA levels in visceral fat only. We were unable to find such associations for adipoR1 mRNA levels in the overall gilt population. The current study demonstrated that TNFalpha downregulates adiponectin and adipoR2, but not adi-poR1, mRNA levels in stromal-vascular cell culture. Moreover, leptin significantly decreased adiponectin mRNA levels, whereas there was no effect on adiponectin receptors. We conclude that adiponectin and adi-poR2 mRNA levels, but not adipoR1, are modulated in pig visceral fat tissues. Furthermore, our results indicate that TNFalpha interferes with adiponectin function by downregulation of adipoR2 but not of adipoR1 mRNA levels in pigs. 相似文献
17.
试验旨在探讨从江香猪肌内前体脂肪细胞分化过程中相关基因的表达。采集3日龄从江香猪背最长肌,采用Ⅱ型胶原酶消化法分离肌内前体脂肪细胞,进行原代和传代培养,并对其进行形态学观察。诱导培养后,利用油红O染色法对其进行鉴定。采用实时荧光定量PCR方法检测细胞诱导分化0、24、48、72和144 h时脂肪相关基因丙酮酸脱氢酶激4（PDK4）、成纤维细胞生长因子10（FGF10）、脂联素（ADIPOQ）、脂肪酸合成酶（FAS）、脂蛋白脂酶（LPL）、CCAAT增强子结合蛋白α（C/EBPα）、脂肪细胞脂肪酸结合蛋白4（FABP4）、蛋白激酶B（AKT2）的表达,选择诱导0 h作为对照组。结果显示,分离的肌内前体脂肪细胞5 h开始贴壁,贴壁的细胞呈圆形,胞体透明,经传代后,细胞形态均一,经诱导培养后,油红染色呈红色。实时荧光定量PCR结果显示,PDK4、ADIPOQ、C/EBPα、FAS、FABP4和AKT2基因mRNA表达水平在诱导48 h时均呈现较高表达,极显著高于其余各阶段（P<0.01）;FGF10基因mRNA表达水平在诱导24和48 h时均较高;LPL基因mRNA表达水平在诱导72 h时极显著高于对照组（P<0.01）,之后明显下降;PDK4、ADIPOQ和FGF10基因mRNA表达水平在诱导144 h时均极显著低于对照组（P<0.01）;C/EBPα基因mRNA表达水平在诱导144 h时显著高于对照组（P<0.05）;FAS基因mRNA表达水平在诱导144 h时显著低于对照组（P<0.05）;AKT2和LPL基因mRNA表达水平在诱导144 h时与对照组差异不显著（P>0.05）。本试验成功培养了从江香猪肌内前体脂肪细胞,并检测了不同诱导阶段脂肪相关基因的表达情况,为进一步研究从江香猪脂肪代谢和沉积提供参考依据。 相似文献
18.
This experiment was conducted to study the effects of the diets with different energy and protein levels on mRNA expression of glucose transporters in the small intestine and muscle of Tan sheep. A total of 112 healthy Tan sheep (half male and half female) with similar initial live weight were randomly divided into four groups with four replicates per group and seven sheep per replicate. According to the Feeding Standard of Meat-producing Sheep and Goats (NY/T 816—2004),each group was fed diet with different levels of energy and protein respectively:0.84×standard level (group Ⅰ),0.96×standard level (group Ⅱ),1.08×standard level (group Ⅲ) and 1.20×standard level (group Ⅳ). The test period were divided into two stages by body weight of sheep (29-35 and 36-40 kg). At the end of each stage,one sheep was slaughtered at each replicate,and small intestine and muscle samples were collected to study mRNA relative expression of SGLT1,GLUT4 and GLUT5 genes by Real-time PCR. The results indicated that:SGLT1 gene mRNA expression levels of group Ⅲ was significantly higher than other groups in small intestine at the end of 29-35 kg stage (P< 0.05);At the end of 36-40 kg stage,the SGLT1 gene mRNA expression levels had no significant difference among the four groups (P> 0.05).In muscle,the SGLT1 gene mRNA expression level increased with the rise of energy and protein levels at both stages ,and that of group Ⅳ were the highest.In small intestine,at the end of 29-35 kg stage,the GLUT4 gene mRNA expression levels had no significant difference among the four groups (P> 0.05);At the end of 36-40 kg stage,the GLUT4 gene mRNA expression level increased with the rise of energy and protein levels,and that of group Ⅳ were significantly higher than the other groups (P< 0.05).In muscle,the GLUT4 gene mRNA expression level were the highest in group Ⅳ at both stages,and significantly higher than group Ⅰ (P< 0.05). The GLUT5 gene mRNA expression did not show any regularity at both stages. In conclusion,diets with different levels of energy and protein could significantly affect the mRNA expression of SGLT1 and GLUT4 genes,and affect absorption of glucose in sheep. 相似文献