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文章介绍了我国塔里木盆地的驴资源和驴用途,以及当前发展养驴业在新疆的重要意义。同时概述了我国驴的福利和健康问题,以及部分国内外对驴寄生虫的研究成果。建议应从流行病学调查为起始点,首先掌握塔里木盆地驴寄生虫的流行规律,为驴寄生虫病的科学防控提供依据。  相似文献   

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饲料中马、驴源性成分的分子生物学检测技术   总被引:1,自引:0,他引:1  
根据马、驴线粒体DNA中的保守区段设计了一对引物,通过聚合酶链式反应(polymerase chain reaction,PCR)可以专一地检测扩增出马、驴源性成分的DNA片段,再经限制性内切酶Sau 3A和Alu Ⅰ的酶切鉴定可以区分马源性成分和驴源性成分.PCR扩增产物的测序结果验证了酶切鉴定结果的正确性。引物灵敏度测试培果表明该方法的检测低限均达0.3%.该对引物可以成为检测马、驴源性成分高效准确的检测工具。  相似文献   

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[目的]开发一种适合阿胶产业化生产的高效驴皮脱毛方法。[方法]低温条件下(4 ℃),选用1%碱性蛋白酶和1%中性蛋白酶对醒发的干驴皮开展酶解脱毛试验,比较2种酶解方法处理下驴皮的脱毛时间、脱毛率,并对脱毛过程中驴皮的气味进行评价。以1%碱性蛋白酶和1%中性蛋白酶酶解处理驴皮以及机械脱毛鲜驴皮为材料熬制阿胶,比较不同原料熬制阿胶的出胶量、出胶率、外观、气味等;测定以2种蛋白酶酶解处理的驴皮为原料熬制的阿胶质量指标,包括水分、灰分、水不溶物以及4种氨基酸含量,并与《中国药典》2015版一部中提供的相应质量标准进行对比。[结果]低温条件下(4 ℃),利用1%碱性蛋白酶(pH值=10.5)或1%中性蛋白酶(pH值=7.5)酶解处理驴皮第2天表现出脱毛效果,处理第5天醒发的干驴皮脱毛率可达90%,有臭味产生但非驴皮腐败臭味,而是脱毛过程中产生的正常气味。以1%碱性蛋白酶、1%中性蛋白酶酶解脱毛后的驴皮以及机械脱毛鲜驴皮为原料熬制阿胶,出胶量分别为113.0、109.4、129.3 g,出胶率分别为13.8%、13.1%、13.2%;2种酶解处理驴皮熬制的阿胶色红、气味芳香,与鲜驴皮熬制的阿胶相比色泽更鲜亮。2种酶解处理驴皮熬制阿胶的水分、灰分、水不溶物均符合并优于《中国药典》2015版一部中的质量标准,L-羟脯氨酸、甘氨酸、丙氨酸、L-脯氨酸含量也远高于《中国药典》2015版一部中的质量标准。[结论]低温条件下(4 ℃),1%碱性蛋白酶和1%中性蛋白酶可对醒发干驴皮进行有效酶解脱毛,且脱毛后驴皮熬制的阿胶质量符合《中国药典》2015版一部标准。  相似文献   

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马、驴精液的保存与应用技术发展速度相对较慢,文幸从马、驴精液的采集,处理,稀释,冷冻,解冻,授精等几个方面就目前国内外发展情况进行了概述.  相似文献   

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驴的精液冷冻保存技术是在马的基础上逐渐发展起来的,特别在近十年来,有关研究明显增多,在诸多方面取得了积极的成果。文章主要以驴精液冷冻保存技术环节的发展为侧重点,就其研究成果进行了总结。  相似文献   

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试验旨在研究泌乳驴的日粮蛋白水平对驴驹生长发育的影响。选取健康、体况良好的5岁龄、胎次(1.9±0.4)胎、体重(240±30) kg、泌乳日期(29±5) d的带驹德州三粉驴24头,分为3个处理组(H、M、L组),饲喂蛋白水平分别为13.18%、12.22%和11.34%的日粮。每个处理组4个重复,每个重复2头驴。正式试验期8周,分为试验前期(1~4周)和试验后期(5~8周)。同时,将24头驴驹跟随母驴相应分为3个处理组,驴驹的公、母比例一致,日龄(29±5) d与体重(48±8) kg相近。所有驴驹除摄取母乳外,补饲数量相同的精补料与粗饲料。试验表明,试验前期泌乳驴的日粮蛋白水平对驴驹日增重无显著的影响(P>0.10);试验后期,随着泌乳驴日粮蛋白水平的增加,驴驹日增重显著增加(P<0.05),饲料转化效率呈一定增加趋势,但对体尺无显著影响(P>0.10)。饲喂泌乳驴高蛋白水平日粮对驴驹血浆中天冬氨酸(Asp)、苏氨酸(Thr)、丝氨酸(Ser)和组氨酸(His)的含量有明显的促进作用。提高泌乳驴的日粮CP水平,对驴驹日增重具有一定促进作用,以12.22%和13.18%的效果较好。  相似文献   

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Reference intervals (RIs) for haematology and serum biochemistry for donkeys in a temperate climate have been previously published using blood sample results from the resident population of a large donkey shelter in the UK. Periodic review of reference intervals is recommended to ensure their applicability to the patient population and changes in laboratory methods and technologies. The current study aimed to revise the previously published haematology and serum biochemistry values for the adult domestic donkey (Equus asinus) in the UK in the light of a change in analytical equipment at the Donkey Sanctuary laboratory, but also to refine the demography of the sample population with respect to age, physiology and clinical history. Clinical pathology results from 138 clinically healthy mature (4–24 years inclusive) female and castrated male donkeys selected from the resident population of the Donkey Sanctuary, were analysed retrospectively. The animals were blood sampled during the period February 2008 to June 2011 as part of a routine health screen prior to rehoming. Results for a total of 38 biochemical and haematological parameters were analysed including 3 previously unreferenced parameters in addition to those assessed in the previous study. The new reference intervals and median values show very poor transferability with recently derived reference intervals for non‐Thoroughbred horses and only limited transferability with reference intervals previously published for donkeys in the UK. Of particular note is a marked reduction in the upper reference limit for triglycerides of 2.8 mmol/l (from 4.3 mmol/l) since this parameter is used to decide when donkeys are at risk of developing hyperlipaemia. This study demonstrates the value of intermittent review of reference intervals and refinement of study populations. Notwithstanding the caution with which reference interval data from different laboratories should be compared, the lack of transferability of results between donkeys and horses highlights the importance of use of species‐appropriate reference intervals for clinical decision‐making.  相似文献   

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Reason for performing study: There is limited information on the gross tracheal morphology of donkeys with or without tracheal abnormalities. Objectives: To: 1) examine the morphology of tracheas of donkeys with and without clinical and/or post mortem evidence of tracheal obstruction; 2) record the cross‐sectional dimensions and shapes of tracheal rings at fixed sites; and 3) document prevalence, sites and characteristics of detected tracheal abnormalities. Methods: The tracheas of 75, predominantly aged (median age 30 years, range 7–48 years) donkeys that died or were subjected to euthanasia on humane grounds were examined. Five had severe dyspnoea due to tracheal obstruction (with intercurrent lung disease in 3), while 7 had post mortem evidence of severe tracheal airway obstruction. Every 5th tracheal ring was dissected free and the inner and outer vertical and transverse dimensions and cross sectional areas were measured. Each dissected ring was photographed and the shape of the trachea was classified as normal or, in one of 6 abnormal grades, according to the type and degree of structural abnormality present. Results: The tracheas had a mean of 43 (range 34–50) tracheal rings that tended to be more oval in shape in the distal cervical region. Only 31.2% of rings examined had a circular to oval shape. Dorso‐ventral flattening was present in 0.9% of tracheal rings, dorsal ligament separation in 24.4%, slight cartilage deformity in 26.0%, moderate cartilage deformity in 10.4%, marked cartilage deformity in 1.9% and miscellaneous other abnormalities in 4.9% of tracheal rings. The 12 donkeys with ante or post mortem evidence of tracheal obstruction had significantly increased tracheal abnormality grade in comparison to the remaining donkeys. Conclusions and potential relevance: Structural tracheal abnormalities are present in most old donkeys, but generally do not cause clinical problems in these sedentary animals unless intercurrent pulmonary disease is present.  相似文献   

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Roundworms and flatworms that affect donkeys can cause disease. All common helminth parasites that affect horses also infect donkeys, so animals that co‐graze can act as a source of infection for either species. Of the gastrointestinal nematodes, those belonging to the cyathostomin (small strongyle) group are the most problematic in UK donkeys. Most grazing animals are exposed to these parasites and some animals will be infected all of their lives. Control is threatened by anthelmintic resistance: resistance to all 3 available anthelmintic classes has now been recorded in UK donkeys. The lungworm, Dictyocaulus arnfieldi, is also problematical, particularly when donkeys co‐graze with horses. Mature horses are not permissive hosts to the full life cycle of this parasite, but develop clinical signs on infection. In contrast, donkeys are permissive hosts without displaying overt clinical signs and act as a source of infection to co‐grazing horses. Donkeys are also susceptible to the fluke, Fasciola hepatica. This flatworm can be transmitted, via snails and the environment, from ruminants. As with cyathostomins, anthelmintic resistance is increasing in fluke populations in the UK. A number of the anthelmintic products available for horses do not have a licence for use in donkeys, and this complicates the design of parasite control programmes. As no new equine anthelmintic classes appear to be near market, it is important that the efficacy of currently effective drugs is maintained. It is important that strategies are used that attempt to preserve anthelmintic efficacy. These strategies should be based on the concept that the proportion of worms in a population not exposed to anthelmintic at each treatment act as a source of ‘refugia’. The latter is an important factor in the rate at which resistance develops. Thus, it is imperative that parasite control programmes take into account the need to balance therapy to control helminth‐associated disease with the requirement to preserve anthelmintic effectiveness.  相似文献   

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ObjectiveTo evaluate the cardiopulmonary effects of anaesthesia induced and maintained with propofol in acepromazine pre-medicated donkeys.Study designProspective experimental study.AnimalsSix healthy male donkeys weighing 78–144 kg.MethodsDonkeys were pre-medicated with intravenous (IV) acepromazine (0.04 mg kg−1). Ten minutes later, anaesthesia was induced with IV propofol (2 mg kg−1) and anaesthesia maintained by continuous IV infusion of the propofol (0.2 mg kg−1 minute−1) for 30 minutes. Baseline measurements of physiological parameters, and arterial blood samples were taken before the acepromazine administration, then 5, 15, 30, 45, and 60 minutes after the induction of anaesthesia. Changes from baseline were analysed by anova for repeated measures.ResultsWhen compared with baseline (standing) values, during anaesthesia heart rate increased throughout: significant at 5 (p = 0.001) and 15 (p = 0.015) minutes. Mean arterial blood pressure increased significantly only at 15 minutes (p < 0.001). Respiratory rate and arterial pH did not change significantly. PaO2 was lower throughout anaethesia, but this only reached significance at 15 minutes (p = 0.041). PaCO2 was statistically (but not clinically) significantly reduced at the times of 30 (p = 0.02), 45 (p = 0.01) and 60 (p = 0.04). Rectal temperature decreased significantly at all times of the study.Conclusions and clinical relevanceAdministration of propofol by the continuous infusion rate for the maintenance of anaesthesia resulted in stable cardiopulmonary effects and could prove to be clinically useful in donkeys.  相似文献   

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Therapeutics are often administered to donkeys based on dosage and intervals recommended for horses because very few drugs have donkey‐specific label indications. Yet differences between donkeys and horses in drug distribution, metabolism and elimination have been noted for most therapeutic agents studied. These differences can be partially explained by the donkey's unique physiology. Since their ancestors evolved in a desert environment, the modern donkey exhibits qualities that allow them to tolerate dehydration better than the horse and recover more quickly from its effects. Fluid balance and body water compartment partitioning differ from the horse and may have implications regarding drug distribution. Since donkeys are preferential browsers, differences in diet may have influenced evolutionary differences in metabolic disposition of drugs. It is important to acknowledge these differences when designing dose regimes for donkeys based on horse protocols in order to avoid either lack of efficacy or toxicity.  相似文献   

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马程  张莉 《家畜生态学报》2021,42(2):8-13,85
动物福利是国内外学者热议的话题,也是标准化生产和畜产品质量安全的重要影响因素.动物福利体现在饲养、养殖环境、健康、行为等各个环节中.马驴作为中国重要的草食家畜,在精准扶贫和乡村振兴中发挥着重要作用.该文阐述了马驴养殖的经济价值,对中国马驴养殖福利现状和动物福利在马驴养殖中的相关影响因素进行探讨,为宣传、提升马驴养殖福利...  相似文献   

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以驴奶为研究对象,测定了其中K、Ca、Na、Mg、Fe、Zn6种微量元素的含量,以期为驴奶资源的开发和利用提供一定的理论参考。  相似文献   

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The aim of the present study was to fill the void in data related to hematological and biochemical parameters of donkey foals. Whole-blood and plasma samples were obtained from 16 Amiata donkey foals at birth, at 24 and 48 hours, and at 1, 2, 3, 4, 6, and 8 weeks of age. RBC, WBC, hemoglobin concentration (Hgb), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, platelet count, glucose, creatinine, blood urea, triglycerides, total cholesterol, total bilirubin, aspartate aminotransferase, γ-glutamyl transferase, creatine-phosphokinase, alkaline phosphatase, total calcium, potassium, sodium, phosphorus, and albumin were measured. Similar to equine foals, values of RBC, Hgb, and Hct decreased significantly after the first 48 hours of life in Amiata donkey foals, reaching values similar to adult donkeys. No changes were found for mean corpuscular volume and mean corpuscular hemoglobin concentration during the study period. The WBC was low at birth when compared with subsequent measurements, but significantly increased in the subsequent surveys. Platelet count was lower in the first week, with a secondary peak 2 weeks later, and then a decline again up to the eighth week. In agreement with equine foals, electrolyte concentrations, triglycerides, and total cholesterol did not show significant differences. Creatinine, total bilirubin, and blood urea showed a trend similar to RBC, Hgb, and Hct. For the first time, data of hematological and biochemical parameters in Amiata donkey foals are provided.  相似文献   

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运用无创伤性检查技术对35匹健康凉州驴和45匹病驴舌色进行了测定,以探讨舌色与血液粘度和血液微循环之间的关系。结果:与正常舌色(淡红色)相比,红绛舌时,血液粘度增高(P<0.01),微循环半更新时间(MRT)、微循环平均滞留时间(MST)延长(P<0.05,P<0.01),而在其他病理舌色时差异不显著(P>0.05)。与淡白舌相比,血液粘度在红绛舌、红舌时增高(P<0.01);MRT、MST在红绛舌时延长(P<0.01)。在其他病理舌色之间,血液粘度、MRT、MST差异均不显著(P>0.05)。  相似文献   

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驴发情期卵泡变化初探   总被引:2,自引:0,他引:2  
采用实时B型超声显像法,初步观察了4头母驴3个发情期卵泡的发育过程,以确定其排卵和输精时间。结果表明:驴卵泡直径大约每天增加1.5~3 mm,优势卵泡平均每天增长2.1 mm,发情周期在20~24 d之间,发情一般持续5~7 d,约在发情停止前1 d排卵。体格较大的驴发情期间卵泡数比体格较小的驴卵泡数多。  相似文献   

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This case report outlines the successful management of a case of chronic grass sickness in a donkey and highlights that the occurrence of grass sickness may be underestimated in this species. Furthermore, grass sickness should be considered in the differential diagnosis in cases of inappetance and ill thrift in donkeys.  相似文献   

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