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1.
利用来源于同一猪场的2头猪瘟病毒(HCV)持续感染的带毒母猪及所产35头仔猪(包括13头死胎)和6头阴性对照猪,观察母猪的胎儿发育成活状况、仔猪HCV带毒率及HCV垂直传播对仔猪猪瘟兔化弱毒疫苗(HCLV)免疫效力的干扰作用,同时进行水平传播试验和观察HCV持续感染对母猪繁殖功能的影响。结果表明：HCV持续感染对其中1头母猪的胎儿发育和成活有明显影响,而对另1头母猪的胎儿发育没有明显影响;HCV持续感染母猪可经过胎盘垂直传播病毒给仔猪,传播率达45％～86％;吃初乳和接种HCLV不能阻止带毒仔猪的死亡,9头带毒仔猪在45d内死亡4头;免疫HCLV不能使带毒仔猪产生免疫保护力。5头猪在强毒攻击后死亡4头;HCV垂直传播的带毒猪可发生水平传播,并引起3／4感染猪死亡;HCV持续感染可引起母猪生殖系统病理变化。导致繁殖障碍。  相似文献   

2.
为了解猪瘟病毒感染仔猪免疫猪瘟疫苗后带毒情况,并比较实验室几种猪瘟抗原检测方法的适用性,采用(CSFV)RT-nPCR、猪瘟兔化弱毒疫苗荧光定量RT-PCR(HCLV-FQ-PCR)和CSFV实时荧光定量RT-PCR(CSFV-FQ-PCR)3种检测方法对田间感染CSFV仔猪疫苗免疫前后带毒情况进行定期跟踪检测.结果显示:本实验室建立的CSFV-FQ-PCR灵敏度高于CSFV-RT-nPCR;猪瘟疫苗免疫48 d后,采用HCLV-FQ-PCR方法检测不到血液中的HCLV;猪瘟病毒感染猪免疫疫苗后仍存在持续带毒现象,因此对猪瘟病毒感染猪必须彻底淘汰.  相似文献   

3.
猪瘟活疫苗(种毒)鉴别检验阳性血清制备   总被引：1，自引：0，他引：1  
为制备猪瘟活疫苗(种毒)鉴别检验阳性血清,本实验将4头非免疫健康猪进行猪瘟活疫苗基础免疫和猪瘟石门强毒株反复强化免疫后,采血分离血清,过滤、分装、冻干、熔封,获得4批产品,并进行了检验和验证。结果显示:物理性状、无菌检验、安全检验、均一性检验、支原体检验、真空度测定均合格;剩余水分均小于4%;特异性检验表明,口蹄疫病毒、猪伪狂犬病病毒、猪繁殖与呼吸综合征病毒、猪细小病毒、牛病毒性腹泻/粘膜病病毒抗体均为阴性;残余猪瘟病毒免疫荧光和套式RT-PCR核酸检测结果均为阴性;兔体中和效价分别为1∶800、1∶800、1∶800和1∶1 600;对猪瘟兔化弱毒种毒和5种猪瘟活疫苗的验证结果均良好。该4批血清可以用于猪瘟活疫苗(种毒)鉴别检验。  相似文献   

4.
猪瘟(CSF)被世界动物卫生组织(OIE)列为必须报告的A类传染病之一,是我国目前强制免疫的主要传染病之一.做好猪瘟免疫是制定猪场免疫程序的基础.近年来,猪瘟疫苗免疫效果不佳甚至免疫失败的现象时有发生,除了猪瘟疫苗质量、免疫程序不当和猪瘟野毒持续感染母猪将其野毒传播给仔猪造成免疫失败等因素外,其他猪病病原感染对猪瘟疫苗的免疫效力也构成影响[1-4].由猪细小病毒(PPV)、猪伪狂犬病病毒(PRV)和猪繁殖与呼吸综合征病毒(PRRSV)单独或混合感染对猪瘟弱毒疫苗免疫效力的影响已有报道[5-8],而由猪圆环病毒(PCV)感染是否猪瘟免疫有影响还鲜有报道.  相似文献   

5.
选择猪繁殖与呼吸综合征病毒和抗体阴性、猪伪狂犬病抗体阴性、猪瘟抗原阴性的20头仔猪,随机分成4组,分别为试验Ⅰ、Ⅱ、Ⅲ组及对照组,分别设计3种猪瘟、猪伪狂犬病和猪繁殖与呼吸综合征免疫程序,首免后第2周开始每隔2周分别采血检测抗体。试验结果表明只有试验Ⅰ组的免疫程序可行,即:21日龄进行猪瘟疫苗首免,60日龄加强免疫1次,母猪分娩前10日龄进行猪伪狂犬疫苗首免,35日龄加强免疫1次,35日龄进行猪繁殖与呼吸综合征疫苗首免,60日龄加强免疫1次。  相似文献   

6.
选择猪繁殖与呼吸综合征病毒和抗体阴性、猪伪狂犬病抗体阴性、猪瘟抗原阴性的20头仔猪,随机分成4组,分别为试验Ⅰ组、Ⅱ组、Ⅲ组及对照组,分别设计3种猪瘟、猪伪狂犬病和猪繁殖与呼吸综合征免疫程序,首免后第2周开始每隔2周分别采血检测抗体.试验结果表明只有试验Ⅰ组的免疫程序可行,即：21日龄进行猪瘟疫苗首免,60日龄加强免疫1次,母猪分娩前10日龄进行猪伪狂犬疫苗首免,35日龄加强免疫1次,35日龄进行猪繁殖与呼吸综合征疫苗首免,60日龄加强免疫1次.  相似文献   

7.
以猪瘟兔化弱毒细胞苗分别免疫3组初生仔猪，每头1.5头剂。第1组20头先免疫，1小时后吸初乳。第2组15头，吸初乳后1小时免疫。第3组13头，吸初乳后半小时免疫。75日龄，以猪瘟弱毒单抗酶联方法测定3组动物的平均抗体(OD值)分别为0．25、0．23及0．21。80日龄，每组各随机抽出4头猪，连同非免疫对照猪6头以猪瘟石门系强毒攻击后，对照猪全死，第1组100％保护，第2组75％保护，第3组50％保护。第1组的免疫效果明显优于其它两组。  相似文献   

8.
猪瘟不同顺序零天免疫的免疫效果比较   总被引：12，自引：0，他引：12  
以猪瘟兔化弱毒细胞苗分别免疫3组初生仔猪，每头1．5头剂。第1组20头先免疫，1小时后吸初乳。第2组15头，吸初乳后1小时免疫。每3组13头，吸初乳后半小时免疫。75日龄，以猪瘟弱毒单抗酶联方法测定3组动物的平均抗体（OD值）分别为0．25、0．23及0．21。80日龄，每组各随机抽出4头猪，连同非免疫对照猪6头以猪瘟石门系强毒攻击后，对照猪全死，第1组100％保护，第2组75％保护，第3组50％保护。第1组的免疫效果明显优于其他两组。  相似文献   

9.
以猪瘟免化弱毒细胞苗分别免疫5组初生仔猪，每头1．5头剂。第1组20头先免疫，1小时后吸初乳。第2组15头，吸初乳后1小时免疫。第5组15头，吸初乳后半小时免疫。75日龄，以猪瘟弱毒单抗酶联方法测定1组动物的平均抗体（OD值）分别为0．25、0．25及0．21。80日龄，每组各随机抽出4头猪，连同非免疫对照猪6头以猪瘟石门系强毒攻击后，对照猪全死，第1组100％保护，第2组75％保护，第5组50％保护。第1组的免疫效果明显优于其他两组。  相似文献   

10.
为了评价猪瘟E2亚单位疫苗单剂量免疫效果,本试验在试验猪场用猪瘟E2亚单位疫苗与猪瘟减毒活疫苗分别免疫供试猪,并定期测定猪瘟病毒抗体。饲养至24周龄时,每组随机选取5头运至检验动物房,用猪瘟石门系强毒进行人工感染。根据试验猪攻毒后临床症状、病理剖检和猪瘟抗原、抗体检测结果,比较分析了猪瘟E2亚单位疫苗单剂量免疫保护力。结果显示,对照组的试验猪攻毒后12 d内全部死亡,并表现出典型的猪瘟症状和病理变化;免疫猪全部存活,未见猪瘟症状和肉眼可见的组织器官病变,血样的猪瘟抗原ELISA检测结果均为阴性。结果表明,猪瘟E2亚单位疫苗单剂量免疫1次后,24周仍能为靶动物提供可靠的免疫保护效力,与猪瘟减毒活疫苗2次免疫的保护效力相当。  相似文献   

11.
运用ELISA、LAT和IHA对湖北省2006年度规模化猪场猪伪狂犬病(PR)、猪繁殖与呼吸综合征(PRRS)、猪乙型脑炎(JE)、猪细小病毒病(PP)、猪瘟(HC)、猪圆环病毒病(PCV-2)及弓形体病进行了血清学检测。分析调查结果发现PR、PRRS、JE、PP 、HC ５种疾病的免疫抗体平均阳性率分别为84.3%、78.3%、84.7%、58.0%、79.7%。非免PRRSV和PRV野毒的平均感染率分别为55.1%和17.7％,表明PRRSV感染情况比较严重。对58场次发病猪场的541份血清进行PRV、PRRSV、PCV-2混合感染情况的调查发现,规模化猪场中普遍存在二重或三重感染,从而使疾病更加复杂化,给防疫工作带来更大的困难。  相似文献   

12.
Four pigs were inoculated subcutaneously with a detergent (triton X 100) split hog cholera virus in Freund’s incomplete adjuvant. Four other pigs were in the same way inoculated with a detergent split bovine viral diarrhoea virus, also in Freund’s incomplete adjuvant. In the experiment were used 3 control pigs. The vaccinations were repeated after 3 weeks. All pigs were challenged with highly virulent hog cholera virus (Tübingen) 12 weeks after primary inoculations. Signs of hog cholera were only noted in the control pigs.This introductory experiment was succeeded by a larger experiment with subcutaneous inoculations of: 10 pigs with detergent split hog cholera virus in Freund’s incomplete adjuvant, 10 pigs with detergent split hog cholera virus in a saponin (Quil A) solution, 10 pigs with detergent split bovine viral diarrhoea virus in Freund’s incomplete adjuvant, 10 pigs with detergent split bovine viral diarrhoea virus in the Quil A solution plus 5 control pigs. The vaccinations were repeated after 3 weeks, and finally all pigs were challenged 9 weeks later with the highly virulent hog cholera virus strain.With the exception of 1 animal which died accidentally, all animals survived in the groups inoculated with the Quil A vaccines and in the group inoculated with the detergent split hog cholera virus/oil adjuvant vaccine. In the group inoculated with the detergent split bovine viral diarrhoea virus/oil adjuvant vaccine, some of the pigs died of hog cholera.  相似文献   

13.
To determine the immune responses in pigs to hog cholera virus after treatment with an ionized alkali mineral complex (IAMC), 40 healthy pigs (28-32 days old) from a commercial swine farm were purchased and housed into 4 groups (n=10 each). All pigs were vaccinated intramuscularly (1 ml) with an attenuated live hog cholera virus (HCV, LOM strain) at 28-32 days old and challenged with a virulent hog cholera virus at 8 weeks after vaccination. Each group was treated with PowerFeel sprayed diet as 0.05% (w/w) in a final concentration (T-1, n=10), a diet mixed with SuperFeed as 3% (w/w) in a final concentration (T-2, n=10), or a diluted PowerFeel solution (1:500, v/v) as drinking water (T-3, n=10), respectively. A group (n=10) served as a non-treated control. Proportions of expressing CD2+ and CD8+ cells increased significantly (p<, 0.05) at 8-week post-application. Mean antibody titers of each group against HCV gradually increased to higher levels after vaccination and with challenge of the virulent virus. In conclusion, the IAMC-treated diets can be helpful for the improvement of growth in pigs with proper vaccination program, while the IAMC-treated diets have no effects on the clinical protection against hog cholera.  相似文献   

14.
Twenty 6-week-old specific pathogen-free pigs were divided into four groups. On day 0 of the experiment, PRRSV-PRV (n = 6) and PRRSV (n = 4) groups were intranasally inoculated with porcine reproductive and respiratory syndrome virus (PRRSV) (10(5.6) TCID50). On day 7, the PRRSV-PRV and PRV (n = 6) groups were intranasally inoculated with pseudorabies virus (PRV) (10(3.6) TCID50). Control pigs (n = 4) were kept as uninoculated negative controls. Half of the pigs in each group were euthanized and necropsied on day 14 or 21. Clinical signs such as depression and anorexia were observed in the PRRSV-PRV and PRV groups after inoculation with PRV. Although febrile response was observed after virus inoculations, the duration of that response was prolonged in the PRRSV-PRV group compared with the other groups. The lungs in the PRRSV-PRV group failed to collapse and were mottled or diffusely tan and red, whereas the lungs of the pigs in the other groups were grossly normal. Histopathologically, interstitial pneumonia was present in all PRRSV-inoculated pigs, but the pneumonic lesions were more severe in the PRRSV-PRV group. Mean PRRSV titres of tonsil and lung in the PRRSV-PRV group were significantly (P < 0.05) higher than that in the PRRSV group on day 21. These results indicate that dual infection with PRRSV and PRV increased clinical signs and pneumonic lesions in pigs infected with both viruses, as compared to pigs infected with PRRSV or PRV only, at least in the present experimental conditions.  相似文献   

15.
Pseudorabies virus (PRV) has been developed as a vaccine vector for expressing foreign immunogens. Porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV), continues to be a major problem to the pork industry worldwide. Many vaccine strategies have been developed to control the disease but most of them turn out to be unsuccessful. The objective of this research was to explore the feasibility of PRV-based vector vaccine in protection against PRRSV. A live attenuated vaccine-based PRV recombinant expressing the envelope protein GP5 of PRRSV was generated using recombinant DNA techniques. The Bartha-K61-derived recombinant virus, named rPRV-GP5, was shown to express PRRSV GP5 efficiently. Sixteen healthy piglets were assigned to one of four groups (one to four, four pigs per group). Animals in Groups 1 and 2 were each inoculated intramuscularly and intranasally with 10(7.0) PFU of rPRV-GP5 and its parent Bartha-K61, respectively; Group 3 were vaccinated intramuscularly with one-dose of PRRS inactivated vaccine; Group 4 was served as non-vaccinated control. One month later, all animals were all challenged with 10(6.5) TCID(50) of virulent PRRSV CH-1a. All animals in Groups 1 and 3 remained clinically healthy before and after challenge, with only a short period of fever (no more than 41 degrees C and 3 days), mild and gradually improving lung and kidney lesions, and short-term viremia (2 and 3 week, respectively) in spite of no detectable anti-PRRSV antibody before challenge. On the other hand, all animals in the other two groups showed evident clinical signs with higher temperatures (more than 41 degrees C) after challenge, and severe lung, kidney and spleen lesions and extended viremia (4 weeks). The results indicate that the rPRV-GP5 is safe for vaccinates and able to confer significant protection against clinical disease and reduce pathogenic lesions induced by PRRSV challenge in vaccinated pigs.  相似文献   

16.
为了建立能够同时检测猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪伪狂犬病病毒(PRV)、猪圆环病毒2型(PCV2)和猪细小病毒(PPV)的多重PCR,并用于猪场感染情况的动态监控以及临床诊断,根据GenBank中已发表的5种病毒的基因序列,针对CSFV的E2、PRRSV的Nsp2、PRV的gB、PCV2的ORF2和PPV的VP2基因,分别设计了特异性引物。在建立的单项PCR基础上,通过优化反应条件,建立了能同时检测5种病毒的多重PCR,并具有较高的灵敏性和良好的特异性。采用建立的多重PCR对127份疑似病猪的扁桃体活体组织进行检测,检出了5种病毒的存在,其中感染2种及以上病毒的样品比例为38.6%(49/127),表明该方法是一种快速、灵敏、高效的病原学检测手段。  相似文献   

17.
Twenty 6‐week‐old specific pathogen‐free pigs were divided into four groups. On day 0 of the experiment, PRRSV–PRV (n = 6) and PRRSV (n = 4) groups were intranasally inoculated with porcine reproductive and respiratory syndrome virus (PRRSV) (105.6 TCID50). On day 7, the PRRSV–PRV and PRV (n = 6) groups were intranasally inoculated with pseudorabies virus (PRV) (103.6 TCID50). Control pigs (n = 4) were kept as uninoculated negative controls. Half of the pigs in each group were euthanized and necropsied on day 14 or 21. Clinical signs such as depression and anorexia were observed in the PRRSV–PRV and PRV groups after inoculation with PRV. Although febrile response was observed after virus inoculations, the duration of that response was prolonged in the PRRSV–PRV group compared with the other groups. The lungs in the PRRSV–PRV group failed to collapse and were mottled or diffusely tan and red, whereas the lungs of the pigs in the other groups were grossly normal. Histopathologically, interstitial pneumonia was present in all PRRSV‐inoculated pigs, but the pneumonic lesions were more severe in the PRRSV–PRV group. Mean PRRSV titres of tonsil and lung in the PRRSV–PRV group were significantly (P < 0.05) higher than that in the PRRSV group on day 21. These results indicate that dual infection with PRRSV and PRV increased clinical signs and pneumonic lesions in pigs infected with both viruses, as compared to pigs infected with PRRSV or PRV only, at least in the present experimental conditions.  相似文献   

18.
Groups of pigs vaccinated with an inactivated bivalent vaccine containing porcine parvovirus (PPV) and pseudorabies virus (PRV) developed geometric mean titers (GMT) of humoral antibody for each of the viruses as high or slightly higher than those of other groups of pigs that were vaccinated with inactivated monovalent vaccines containing one or the other of the same viruses. An increase in GMT after challenge exposure of vaccinated pigs to live virus indicated that vaccination did not prevent virus replication. However, an indication that replication was less extensive in vaccinated pigs was provided by the following. Although neither vaccinated nor nonvaccinated (control) pigs had clinical signs after exposure to the live PPV, the effect of vaccination was evident by the fact that GMT were higher in nonvaccinated pigs after exposure than they were in vaccinated pigs. Conversely, all pigs exposed to live PRV had clinical signs, but these signs varied between mild-to-moderate and transient for vaccinated pigs to severe and fatal for nonvaccinated pigs.  相似文献   

19.
The aim of this study was to investigate the effects of a porcine reproductive and respiratory syndrome virus (PRRSV) infection on the development of the immune response after pseudorabies virus (PRV) vaccination in pigs. Pigs were intranasally inoculated with the European PRRSV strain, Lelystad virus ter Huurne, and were vaccinated intramuscularly with PRV 2 weeks later (LV-PRV group). Control pigs were vaccinated with PRV only (PRV group). Eight weeks after PRV vaccination, pigs from both groups were challenged intranasally with wild-type PRV. We measured the lymphoproliferative, and the cytolytic responses to PRV of peripheral blood mononuclear cells (PBMC), isolated from blood samples. In addition, serum samples were examined for antibodies against PRV and LV. One week after PRV vaccination, PBMC proliferated abundantly to PRV in both groups. However, in the LV-PRV group the lymphoproliferative response declined after 1 week, whereas, in the PRV group, the lymphoproliferative response was high for 3 weeks and declined thereafter (P<0.05). After challenge, the lymphoproliferative response was 1 week earlier and was consistently and significantly higher in the PRV group than in the LV-PRV group. The PRV-specific killing was higher at 3 weeks after PRV vaccination and 5 weeks after PRV challenge 19+/-3 and 24+/-6%, respectively, in the PRV group, compared to 7+/-4 and 6+/-9%, respectively, in the LV-PRV group (P<0.05). However, later after vaccination and challenge the cytolytic response was identical in both groups. The antibody titre against PRV developed equally in both groups. After challenge, no PRV virus was isolated from both groups. From these results we conclude that, although PRRSV infection did cause changes in the time course of the T-lymphocyte response after PRV vaccination, PRRSV infection did not inhibit the development of vaccine-induced protection after PRV.  相似文献   

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