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1.
黄淮海大豆新品种(系)的抗病性评价   总被引:4,自引:0,他引:4  
大豆花叶病毒(SMV)、大豆孢囊线虫(SCN)和大豆根腐病(SRR)是危害中国乃至世界大豆生产的3种主要病害。为了评价黄淮海选育的大豆新品种(系)对这3种病害的抗性水平, 采用温网室接种鉴定及田间调查自然发病的方法, 对72份大豆新品种(系)进行了抗病性鉴定。结果表明, ‘中作J8012’、‘蒙01-38’、‘汾豆86’、‘中作X96328’、‘阜08-190’等9个品系对SMV流行株系SC3和SC7表现高抗或抗病, 占参试品种总数的12.5%; ‘周01015-1’、‘远育8号’和‘冀豆17’ 3个品系对SCN 1号生理小种表现中抗; 自然条件下, 抗SRR病情指数在15以下的大豆材料有31份, 占鉴定总数的43.06%; 对SMV和SRR的抗性水平均在中抗及以上的有‘阜08-325’、‘HD0113’、‘太丰6号’等8个品系, 占参试品种数的11. 11%, 而对3种病害的抗性水平均在中抗以上的材料仅有‘冀豆17’1份。本研究的结果可为大豆抗病育种选择亲本和培育多抗性的大豆新品种提供参考。  相似文献   

2.
大豆花叶病毒病研究进展   总被引:15,自引:0,他引:15  
 大豆花叶病毒病是世界性病害,导致大豆产量降低并产生种粒斑驳。目前国内外对SMV株系的划分不统一。美国报道了G1-G7,G7A,C14九个株系,日本报道了A-E 5个株系,中国东北1、2、3号株系,江苏SA-SH株系,湖北S1,S2株系,黄淮Y 1-Y7株系。各地学者开展了抗源的鉴定和抗病育种工作,筛选和选育出一批抗病品种。美国已命名3个抗性基因,Rsv1,Rsv2,Rsv3。由于抗源不同,对中国东北3个株系SM V抗性遗传研究结果不同,抗性受1对或2对显性或隐性基因控制;对江苏株系抗性遗传研究结果一致,抗性受单显性基因控制。对感染SM V后大豆植株及种粒生理生化性状的变化及抗性机制进行了研究,表明过氧化物酶同工酶等性状与抗性有关。目前已鉴定出与抗性基因连锁的SSR,RFL P和R APD分子标记,成功的克隆了SMV外壳蛋白基因并导入大豆中。  相似文献   

3.
我国东北部五省市SMV对大豆主栽品种的毒力测定   总被引:9,自引:1,他引:8  
 自东北三省、山东及北京中国科学院遗传所农场(培育黄淮海豆区大豆品种)采集SMV毒株231个,用各地当前大豆主栽品种56个测定毒株的毒力。结果将SMV分为3个株系群,7个亚群,13个株系,即S1,S2-1M,S2-1N,S2-2M,S2-2N,S2-3M,S2-3N,S3-1M,S3-1N,S3-2M,S3-2N,S3-3M,S3-3N。除S1外,各亚群包括引起系统花叶(M)及系统坏死(N)2类株系。大豆品种按抗性归成8类。病毒群体毒力自北向南逐渐增强,黑龙江省的毒株群体毒力最弱,北京的最强,品种抗性也自北向南逐渐增高,黑龙江省大多数为高感品种,吉林多为中感品种,辽宁为中抗品种,北京全属高抗品种,品种抗性水平与病毒群体毒力相互适应。  相似文献   

4.
大豆抗食心虫鉴定和利用研究   总被引:1,自引:0,他引:1  
经过5年对抗大豆食心虫筛选,先后鉴定1411份大豆品种(系),初选出抗级以上品种(系)126个,最后确定豫豆6号为抗虫品种,已在全省大面积推广;抗虫品系12个,其中八八抗084、081等已被育种单位作为抗源杂交育种,繁殖到F_4代。该研究采用异地田间鉴定,异地人工接虫鉴定与产量对比同时进行的方法,缩短了抗虫鉴定和育种的年限,克服了抗虫品种适应性差、产量低的缺点。  相似文献   

5.
闫佳会  姚强  陈海民 《植物保护》2016,42(3):212-214
本研究旨在明确青海青稞主栽品种和后备品种对条纹病和云纹病的抗性,以期为抗病育种及田间病害防治提供理论依据。试验采用田间自然病圃法,对青海主栽的30个青稞品种(系)进行了条纹病和云纹病田间抗性鉴定。结果表明,供试青稞品种(系)对2种病害的抗性存在显著差异,但缺乏免疫品种。对条纹病表现高抗的有13个品种(系),占鉴定总数的43.3%,其中主栽品种有3个,即‘门农1号’、‘昆仑13号’、‘巴青1号’。品系17发病率最低。对云纹病表现高抗的有品系1、品系2、品系6、品系17、品系28、RQKQ-3、RQKQ-5、RQKQ-6、RQKQ-7、RQKQ-8、‘门农1号’、‘互青2号’、‘北青6号’和‘昆仑10号’,共14个,其病情指数均在10以下;表现中抗的共有8个,分别为品系5、品系11、RQKQ-1、RQKQ-9、‘巴青1号’、‘北青3号’、‘北青7号’和‘昆仑13号’。  相似文献   

6.
黄淮区大豆花叶病毒株系组成与分布   总被引:10,自引:0,他引:10  
 用8个大豆品种作为一组株系鉴别寄主,将黄淮豆区8省市202个毒株划分为7个株系(y1~y7)。y1及y2为弱毒株系,仅侵染感病品种(1138-2、文丰5号);y3、y4、y5为中毒株系,分别侵染感病和中抗品种(齐黄10号、徐豆1号、诱变30、鲁豆4号);y6及y7为强毒株系,除侵染以上品种外,还侵染高抗品种(齐黄22、密荚1号)。全区弱毒株系占55.9%,中毒株系占28.7%,强毒株系占15.3%。江苏、山东、河南、河北、安徽和北京市以弱毒株系为主(43.5%~88.9%),山西省以中毒株系为主(50.0%),陕西省弱毒株系、中毒株系各占50.0%,山东、河南、河北3省强毒株系所占比例较高(22.4%~33.3%)。株系分布具有一定的稳定性。  相似文献   

7.
在1985—1990年,对584个大豆品种(系)进行了抗蝇性研究。经初筛、复筛,最后筛选出4个抗性较好的品种,以豫豆2号抗性最好。并测定了大豆品种(系)对豆秆黑潜蝇的扰生性、耐害性。分析了17种氨基酸与抗性的关系;对大豆不同品种主茎进行切片检查发现,髓部直径与主茎直径比值(X_1)、木质部厚度与主茎直径比值(X_2)与侵入虫量(Y)关系密切。其多元回归方程为:log■=1.3499+0.1694X_1-2.0719X_2  相似文献   

8.
北方大豆品种资源抗大豆花叶病毒筛选   总被引:1,自引:0,他引:1  
 大豆花叶病(Soybean Mosaic Virus,简称SMV)是世界各大豆区发生最广,危害最重的病毒病害。鉴定筛选抗大豆花叶病毒的抗性材料,为抗SM V育种提供抗源,有重要的意义。  相似文献   

9.
为明确北部麦区河北、山西育成品种对中国小麦条锈病的抗性水平及部分已定位抗条锈病基因的分布状况, 利用小麦条锈菌当前优势小种CYR32、CYR33和CYR34对89份来自河北、山西的小麦品种在温室进行苗期分小种抗性鉴定, 在小麦条锈病不同流行区甘肃清水、四川郫县、湖北荆州设置鉴定圃进行成株期抗性鉴定; 采用与部分已知基因紧密连锁的分子标记进行抗病基因检测。结果显示, 苗期仅‘长4640’‘晋麦91号’‘科农2009’3份品种对CYR32、CYR33和CYR34均表现抗性; 在甘肃清水、四川郫县、湖北荆州鉴定圃中抗病材料的比例分别为39.33%、24.72%和58.43%, ‘长4640’和‘晋麦91号’为全生育期抗性。通过6个已知抗条锈基因Yr5、Yr9、Yr10、Yr15、Yr18 和Yr26 对89份小麦品种进行分子标记检测, 结果表明, 13份品种检测到Yr9标记, 2份检测到Yr18标记, 未检测到Yr5、Yr10、Yr15和Yr26。以上结果表明, 河北、山西小麦品种对越冬菌源基地的条锈菌的抗性水平很低, 同时成株期抗性基因的利用率低, 今后应加强对新抗病基因的利用, 育种过程中尽可能在不同流行区进行异地鉴定。  相似文献   

10.
大豆花叶病毒(SMV)两个新株系的鉴定   总被引:9,自引:1,他引:9  
1983—1984年在南京本校大豆研究室试验田内的“广吉”品种上分离到K_1和K_2两个病毒分离物。根据它们的寄主范围、体外抗性和蚜虫介体传病特性,电镜观察病毒粒体形态和内含体特征,以及SDS——琼脂双扩散的血清学反应,证实了K_1和K_2两个分离物都是属于马铃薯Y病毒组的大豆花叶病毒(SMV)。在大豆花叶病毒株系鉴别寄主上,K_1和K_2分离物与已鉴定的大豆花叶病毒S_A、S_B、S_C和S_E几个江苏株系的反应不同,主要是K_1和K_2两个株系都能系统侵染大豆“广吉”品种。K_1表现为系统轻斑驳花叶,而K_2为系统枯斑和叶脉坏死。此外,K_1不能侵染大豆品种“齐黄1号”和蚕豆品种“三白”等。以上证明,K_1和K_2两个分离物是江苏大豆花叶病毒(SMV)上的新株系。为了统一大豆花叶病毒的株系编号,将K_1和K_2分离物分别定名为S_G和S_H株系。  相似文献   

11.
Fifteen species of dermestid beetles were recorded at ‘Evolution Canyon’ (EC), Lower Nahal Oren, Mt. Carmel, Israel. They represent ~35% of known Israeli dermestid species. The following three species were recorded for the first time in Israel:Trogoderma svriaca Dalla Torre, 1911;Ctesias svriaca Ganglbauer, 1904; andAnthrenus (s.str.) jordaniens Pic, 1934. Adults of 13 species were collected on the more solar radiated, warmer and climatically more fluctuating south-facing slope (SFS); ten species were collected on the opposite, north-facing slope (NFS), which was cooler and climatically more stable. The abundance of adult dermestid beetles was 1.9 times higher on the SFS than on the NFS (86 and 47, respectively). Species richness and abundance distribution at EC (three collecting stations on each slope and one at the valley bottom) were significantly negatively correlated with the plant cover that consisted of trees and bushes (Spearmanr s ,P=0.007 and 0.039, respectively) and perennials (Spearmanr s ,P=0.039 and 0.077, respectively), indicating that non-woody plants were preferred by adult dermestid beetles.  相似文献   

12.
Molecular diagnostic techniques have been developed to differentiate the Ascochyta pathogens that infect cool season food and feed legumes, as well as to improve the sensitivity of detecting latent infection in plant tissues. A seed sampling technique was developed to detect a 1% level of infection by Ascochyta rabiei in commercial chickpea seed. The Ascochyta pathogens were shown to be genetically diverse in countries where the pathogen and host have coexisted for a long time. However, where the pathogen was recently introduced, such as A. rabiei to Australia, the level of diversity remained relatively low, even as the pathogen spread to all chickpea-growing areas. Pathogenic variability of A. rabiei and Ascochyta pinodes pathogens in chickpea and field pea respectively, appears to be quantitative, where measures of disease severity were based on aggressiveness (quantitative level of infection) rather than on true qualitative virulence. In contrast, qualitative differences in pathogenicity in lentil and faba bean genotypes indicated the existence of pathotypes of Ascochyta lentis and Ascochyta fabae. Therefore, reports of pathotype discrimination based on quantitative differences in pathogenicity in a set of specific genotypes is questionable for several of the ascochyta-legume pathosystems such as A. rabiei and A. pinodes. This is not surprising since host resistance to these pathogens has been reported to be mainly quantitative, making it difficult for the pathogen to overcome specific resistance genes and form pathotypes. For robust pathogenicity assessment, there needs to be consistency in selection of differential host genotypes, screening conditions and disease evaluation techniques for each of the Ascochyta sp. in legume-growing countries throughout the world. Nevertheless, knowledge of pathotype diversity and aggressiveness within populations is important in the selection of resistant genotypes.  相似文献   

13.
Recent data on the epidemiology of the common mycotoxigenic species of Fusarium, Alternaria, Aspergillus and Penicillium in infected or colonized plants, and in stored or processed plant products from the Mediterranean area are reviewed. Emphasis is placed on the toxigenicity of the causal fungal species and the natural occurrence of well known mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, zearalenone, patulin, Alternaria-toxins and moniliformin), as well as some more recently described compounds (fusaproliferin, beauvericin) whose toxigenic potential is not yet well understood. Several Fusarium species reported from throughout the Mediterranean area are responsible of the formation of mycotoxins in infected plants and in plant products, including: Fusarium graminearum, F. culmorum, F. cerealis, F. avenaceum, F. sporotrichioides and F. poae, which produce deoxynivalenol, nivalenol, fusarenone, zearalenone, moniliformin, and T-2 toxin derivatives in wheat and other small grains affected by head blight or scab, and in maize affected by red ear rot. Moreover, strains of F. verticillioides, F. proliferatum, and F. subglutinans, that form fumonisins, beauvericin, fusaproliferin, and moniliformin, are commonly associated with maize affected by ear rot. Fumonisins, were also associated with Fusarium crown and root rot of asparagus and Fusarium endosepsis of figs, caused primarily by F. proliferatum. Toxigenic A. alternata strains and associated tenuazonic acid and alternariols were commonly found in black mould of tomato, black rot of olive and citrus, black point of small cereals, and black mould of several vegetables. Toxigenic strains of A. carbonarius and ochratoxin A were often found associated with black rot of grapes, whereas toxigenic strains of A. flavus and/or P. verrucosum, forming aflatoxins and ochratoxin A, respectively, were found in moulded plant products from small cereals, peanuts, figs, pea, oilseed rape, sunflower seeds, sesame seeds, pistachios, and almonds. Finally, toxigenic strains of P. expansum and patulin were frequently found in apple, pear and other fresh fruits affected by blue mould rot, as well as in derived juices and jams.  相似文献   

14.
We first discuss the diversity of fruit fly (Diptera: Tephritidae) parasitoids (Hymenoptera) of the Neotropics. Even though the emphasis is on Anastrepha parasitoids, we also review all the information available on parasitoids attacking flies in the genera Ceratitis, Rhagoletis, Rhagoletotrypeta, Toxotrypana and Zonosemata. We center our analysis in parasitoid guilds, parasitoid assemblage size and fly host profiles. We also discuss distribution patterns and the taxonomic status of all known Anastrepha parasitoids. We follow by providing a historical overview of biological control of pestiferous tephritids in Latin American and Florida (U.S.A.) and by analyzing the success or failure of classical and augmentative biological control programs implemented to date in these regions. We also discuss the lack of success of introductions of exotic fruit fly parasitoids in various Latin American countries. We finish by discussing the most pressing needs related to fruit fly biological control (classical, augmentative, and conservation modalities) in areas of the Neotropics where fruit fly populations severely restrict the development of commercial fruit growing. We also address the need for much more intensive research on the bioecology of native fruit fly parasitoids.  相似文献   

15.
The genera ofMicrogaster Latreille 1804 andHygroplitis Thomson 1895 from China are presented systematically in this paper. Thirty-two species ofMicrogaster and three species ofHygroplitis are known in China. Diagnosis, character variation, distribution and host of each species among the two genera are presented, including its host and distribution. Keys to the species ofMicrogaster andHygroplitis are given. http://www.phytoparasitica.org posting Dec. 19, 2006.  相似文献   

16.
Plant Viruses Transmitted by Whiteflies   总被引:18,自引:0,他引:18  
One-hundred and fourteen virus species are transmitted by whiteflies (family Aleyrodidae). Bemisia tabaci transmits 111 of these species while Trialeurodes vaporariorum and T. abutilonia transmit three species each. B. tabaci and T. vaporariorum are present in the European–Mediterranean region, though the former is restricted in its distribution. Of the whitefly-transmitted virus species, 90% belong to the Begomovirus genus, 6% to the Crinivirus genus and the remaining 4% are in the Closterovirus, Ipomovirus or Carlavirus genera. Other named, whitefly-transmitted viruses that have not yet been ranked as species are also documented. The names, abbreviations and synonyms of the whitefly-transmitted viruses are presented in tabulated form together with details of their whitefly vectors, natural hosts and distribution. Entries are also annotated with references. Whitefly-transmitted viruses affecting plants in the European–Mediterranean region have been highlighted in the text.  相似文献   

17.
Solacol®, a formulation of the antibiotic validamycin, at 0.33% in 2% malt extract agar, reduced the spread of fungi on dilution plates drastically and allowed twice as much incubation time before subculturing; this resulted in an elevated number of species isolated. Using pure cultures of 62 common soil fungi, it was shown that all fast-growing species (exceptPythium ultimum) were efficiently inhibited but not completely suppressed. Inhibition was comparable to that by 0.5% oxgall, though, while this substance completely suppressed several species, Solacol very strongly inhibited onlyGaeumannomyces graminis, Gerlachia nivalis, Harzia acremonioides, Verticillium biguttatum andRhizoctonia solani. In a further experiment each separate constituent of Solacol was tested against 22 fungi at equivalent concentrations. Validamycin strongly inhibitedChaetomium globosum and two Basidiomycetes, though hardly more than the non-ionic detergent which mainly inhibited the other fungi. A few species were, however, more inhibited by Solacol than by the detergent alone. Solacol at 0.33% is a suitable aid in dilution plating of soil fungi, by increasing the number of colonies and species observed.Samenvatting Solacol®, een formulering van het antibioticum validamycine, remde de groei van schimmels in verdunningsplaten met een concentratie van 0.33% in 2% moutagar en maakte het mogelijk de periode tot afenten met een factor 2 te verlengen; daardoor was het aantal geïsoleerde soorten duidelijk toegenomen. Met reincultures van 62 algemene grondschimmelsoorten werd aangetoond, dat alle snelgroeiende soorten (met uitzondering vanPythium ultimum) voldoende geremd, maar niet volkomen onderdrukt werden. Het remmingspercentage was vergelijkbaar met dat van 0.5% ossegal, hoewel dit laatste sommige soorten volkomen onderdrukte; Solacol remde alleenGaeumannomyces graminis, Gerlachia nivalis, Harzia acremonioides, Verticillium biguttatum enRhizoctonia solani zeer sterk. In een volgend experiment werden de componenten van Solacol t.o.v. 22 fungi apart getoetst in concentraties equivalent aan 0.33% Solacol. Validamycine remde alleenChaetomium globosum en twee basidiomyceten behoorlijk, maar nauwelijks meer dan de niet-ionische uitvloeier, die in hoofdzaak de overige remeffecten veroorzaakte. Enkele soorten werden echter door het complete Solacol veel sterker geremd dan door de uitvloeier alleen. Solacol in een verdunning van 0,33% wordt aanbevolen bij verdunningsplaten voor het isoleren van grondschimmels ten einde het aantal kolonies en soorten te verhogen.  相似文献   

18.
《干旱区科学》2014,(6):782-782
正Journal of Arid Land(JAL)is an international journal(ISSN 1674-6767;CN 65-1278/K)for the natural sciences,sponsored by the Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences and Science Press.It is published by Science Press and Springer-Verlag Berlin Heidelberg bimonthly.JAL publishes original,innovative,and integrative research from arid and semiarid regions,ad  相似文献   

19.
Broad bean mottle virus (BBMV) was transmitted from infected to healthy faba-bean plants by the curculionid weevilsApion radiolus Kirby,Hypera variabilis Herbst,Pachytychius strumarius Gyll,Smicronyx cyaneus Gyll, andSitona lineatus L. The latter appeared to be an efficient vector: acquisition and inoculation occurred at the first bite, the rate of transmission was c. 41%, and virus retention lasted for at least seven days.S. lineatus transmitted the virus from faba bean to lentil and pea, but not to the three genotypes of chickpea tested. This is the first report on the generaHypera, Pachytychius, andSmicronyx as virus vectors, and onA. radiolus, H. variabilis, P. strumarius, andS. cyaneus as vectors of BBMV.Out of 351 samples of food legumes with symptoms suggestive of virus infection, 16, 11, 19, and 17% of the samples of chickpea, lentil, pea, and common bean, respectively, were found infected when tested for BBMV in DAS-ELISA. This is the first report on the natural occurrence of BBMV in chickpea, lentil, pea, and common bean. The virus should be regarded as a food-legume virus rather than a faba-bean virus solely, and is considered an actual threat to food legume improvement programmes.  相似文献   

20.
A collection of 38 PVY isolates from seed potato batches, originating from several Western European countries, was characterized by using current biological, serological and molecular tools differentiating PVY strains and groups. The correlation between the three kinds of tests was good but not absolute. No single serological or PCR method was able to discriminate among the five isolate groups found. Twenty-nine isolates belonged to the PVYN strain and six to the PVYO strain. No PVYC was found. Two other isolates reacted serologically like PVYO, but were unable to elicit a hypersensitive response from the Nytbr gene and probably represent the PVYZ group. At the molecular level, these two isolates showed a combination of both PVYO and PVYN and could be recombinants of these strains. Another isolate reacted serologically like PVYO, but induced vein necrosis in tobacco, like PVYN-Wilga. Some PVYN isolates caused tuber ring necrosis in glasshouse conditions. These might belong to the PVYNTN group. The PVYNTN, PVYN-Wilga and PVYZ groups probably represent pathotypes within strains PVYN and PVYO, respectively. The present study also confirms previous reports showing a high genetic variation at the 5 end within the PVYN strain.  相似文献   

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