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1.
选择棉纤维比强度差异明显的2个品种,研究了棉纤维发育关键酶(蔗糖合成酶和β-1,3-葡聚糖酶)活性的变化特征及其与纤维比强度的关系。结果表明,棉纤维发育过程中蔗糖合酶、β-1,3-葡聚糖酶活性变化特征在生化和mRNA转录水平上均存在明显的差异,影响纤维素的沉积特性及纤维比强度。高强纤维品种(科棉1号,平均比强度为35 cN·tex-1)的蔗糖合酶和β-1,3-葡聚糖酶活性及其基因表达量和维持高表达时间均高于低强纤维品种(德夏棉1号,平均比强度为26 cN·tex-1)。其中,高强纤维品种蔗糖合酶的基因表达量铃龄25 d时明显高于低强纤维品种,而β-1,3-葡聚糖酶的基因表达量则在铃龄10~25 d高于低强纤维品种。在纤维素形成过程中,高强纤维品种的纤维素累积平缓且纤维素累积持续期长于低强纤维品种,品种间差异程度受棉株果枝部位影响。在棉纤维发育过程中,Expansin、β-1,4-葡聚糖酶的基因表达量随铃龄的增加呈下降趋势(铃龄20 d时表达量显著下降),这与棉纤维形成过程(铃龄25 d前伸长较快,随后趋于停止)一致,且高强纤维品种维持高表达时间长与其纤维伸长期较长相吻合。  相似文献   

2.
Colored cotton is a very attractive proposition for the textile industry as it reduces the cost for dying. However, inferior fiber quality and non uniform color make it unsuitable for heavy machine spinning. Due to this reason cultivation of colored cotton on commercial scale has been restricted by cotton growers. The current study was planned to unveil the process of fiber development in isogenic lines of brown, green and white cotton by comparing their fiber length, flavonoid, cellulose, carbohydrate and enzyme activities (SuSy, SPS, acid invertase, Cox, NAD+, NADH, NADP+, and NADPH). White cotton fiber exhibited high cellulose contents (915.81 mg g?1) and long fibers (3.07 cm) as compared to colored cotton fibers with low cellulose content (787.63 and 780.66 mg g?1) and shorter fiber length (2.54 and 2.48 cm). Similarly the amount of flavonoids also varied significantly with maximum concentration (8.67 and 7.13 mg g?1) in brown and green cotton fiber at 5 DPA as compared to white cotton fiber (3.12 mg g?1) at 0 DPA. This high concentration of flavonoid not only lowered the total amount of carbohydrates but also lowered the sucrose transformation rate to developing cotton fiber in colored cotton. The activity of different enzymes (AI, SuSy and SPS, Cox, NAD+/NADH and coenzymes NADP+/NADPH involved in the metabolism of carbohydrates was higher in brown cotton fiber than green cotton fiber. However, high activity of these enzymes did not always correlated with higher amount of cellulose thus indicated the involvement of complex mechanisms for cellulose and flavonoid synthesis during the development of cotton fiber.  相似文献   

3.
高品质陆地棉蔗糖代谢关键酶活性对纤维品质形成的影响   总被引：4，自引：1，他引：3  
以高品质陆地棉鲁324系(324)、渝棉1号(YM-1)和普通陆地棉鲁棉研18 (L18)为材料, 研究蔗糖代谢关键酶活性变化对纤维品质形成的影响。结果表明, 高品质陆地棉叶片蔗糖磷酸合成酶(sucrose phosphate synthetase, SPS)活性高, 蔗糖合成能力强, 主茎叶和果枝叶蔗糖含量高, 蔗糖供应能力强。Logistic方程模拟纤维素累积过程, 高品质陆地棉纤维素进入快速累积期的时间晚, 终止期延后, 快速累积持续期长, 累积速率平缓, 最终纤维素含量高, 纤维比强度高。在纤维发育前期, 高品质陆地棉蔗糖贮存较多, 为次生壁发育提供了充足的初始底物。高品质陆地棉蔗糖合成酶(sucrose synthetase, SS)活性高, 降解蔗糖能力强, 酶活性迅速增加时期与纤维素快速累积期相吻合; SPS活性在纤维素快速累积期终止前均明显高于普通陆地棉。叶片SPS活性和棉纤维中SS、SPS活性与纤维素累积及纤维品质形成有密切关系。  相似文献   

4.
The cotton (Gossypium hirsutum L.) crop is an indeterminate species that is most sensitive to drought stress during the flowering and boll-formation stage of development. To evaluate the effect of water deficit on fibre quality and carbon metabolism at different fruiting branches (FB), multiple controlled experiments were conducted. The cotton cultivar Siza 3 was used under three soil relative water content (SRWC): control (CK, SRWC (75 ± 5) %), moderate drought stress (MDS, SRWC (60 ± 5) %) and severe drought stress (SDS, SRWC (45 ± 5) %) treatment, imposed at the beginning of the flowering of FB6–7. Fibre quality was significantly affected by drought level and fruiting branch location on the plant. Compared to the CK, under MDS and SDS, sucrose content and sucrose synthase (SuSy) activity decreased prior to 24 days post-anthesis (DPA; the fibre elongation phase), which caused low fibre turgor pressure. This decreased the rate of fibre elongation and final fibre length. Moreover, from 24 to 38 DPA (during fibre thickening), low fibre sucrose content led to insufficient UDP-glucose (UDPG; uridine diphosphate glucose) for cellulose synthesis. Furthermore, more of the existing UDPG was allocated to the synthesis of callose instead of cellulose, which resulted in lower cellulose accumulation and decreased fibre strength. The magnitude of the drought stress effect on fibre qualities, as well as the formation process, was strongly influenced by fruiting branch position, where differences were most pronounced for fruiting branches further away from the base of the plant.  相似文献   

5.
高品质陆地棉纤维品质形成特点的研究   总被引：14，自引：7，他引：7  
以常规棉苏棉16为对照,研究了高品质棉渝棉1号、高品质Bt棉科棉1号的纤维发育及品质形成特点。结果表明,与苏棉16相比,科棉1号和渝棉1号纤维快速伸长期略长,快速伸长期内的长度日增长速率较高;比强度快速伸长期较短,但在快速伸长期内的增长速率较高;纤维素含量随着花后天数呈增加趋势,17 d内的增长速率很低,17 d后速率明显加快,至花后24 d呈直线上升趋势,到花后45 d时纤维素含量基本稳定。与苏棉16相比,渝棉1号和科棉1号花后IAA含量较高;花后17 d ABA含量较高,花后24 d的ABA/IAA较高,说明不同基因型纤维发育和品质建成过程的差异与其纤维细胞内的激素变化有关。  相似文献   

6.
Global warming could possibly increase the air temperature by 1.8–4.0°C in the coming decade. To forecast the effects of long‐term elevation of air temperature on sucrose synthesis in cotton leaves at different positions, field experiments with the treatments of ambient temperature (control) and elevated temperature (2–3°C) across cotton's boll forming stage were conducted in 2011 and 2012. Results showed that sucrose content in the fourth main‐stem leaves from the terminal (FMLT) during 10–17 days after treatment (DAT) and in the leaves subtending the cotton boll (LSCB) during 17–45 days post‐anthesis (DPA) was lower in the increased temperature treatment than control treatment, but starch content was higher, resulting in higher specific leaf weight (SLW). Lower sucrose content might be attributed to low ribulose‐1,5‐bisphosphate carboxylase‐oxygenase (Rubisco) and cytosolic fructose‐1,6‐bisphosphatase (cy‐FBPase) activities under elevated temperature for all tested leaves. Comparison of different fruiting branch (FB) positions, sucrose transformation rate just decreased in LSCB growing on FB2–3 and FB6–7. Compared with FB10–11, the leaves at FB2–3 and FB6–7 were more sensitive to the increased temperature, showing as that the changing amplitudes of SLW, sucrose content, starch content, initial Rubisco activity and cy‐FBPase activity were greater. Regarding different types of cotton leaves, the effects of elevated temperature on sucrose content, starch content and initial Rubisco activity in FMLT were obvious at the early stage (10–17 DAT), but were not obvious at the late stage. However, the effects were consistent in LSCB from 17 to 45 DPA. In addition, long‐term elevated temperature had smaller impacts on above indices in FMLT than LSCB. Sucrose phosphate synthase (SPS) and sucrose synthase (SuSy) activities were decreased by elevated temperature in FMLT at the early stage, but were increased in LSCB, suggesting that SPS and SuSy were the key enzymes between FMLT and LSCB in response to elevated temperature.  相似文献   

7.
[Objective] The present study aimed to investigate how sucrose metabolism in the leaf subtending to cotton boll, within-boll yield components and fiber quality respond to varying planting dates. [Method] Two upland cotton lines A705 and A201 differing in cotton boll traits were field tested in 2016-2017. Two different planting dates were designed with early planting on April 12, 2016, and April 15, 2017, and late planting on May 6, 2016, and May 28, 2017, respectively. Dynamics of non-structural carbohydrates (sucrose, hexose, starch) and key sucrose metabolism enzymes in the leaf subtending to cotton boll were examined including vacuolar acid invertase, cell wall acid invertase, sucrose phosphate synthase and sucrose synthase. The differences between two planting dates were compared for within- boll yield components, fiber quality and sucrose metabolism related characteristics. [Result] Late planting lengthened the period of cotton boll maturation relative to early planting. Boll weight, seed mass per boll, seed index and fiber length were increased, and lint percentage and micronaire were decreased. Late planting reduced the activities of cell wall acid invertase and sucrose synthase responsible for sucrose degradation, and in turn led to the increment of sucrose concentration in the subtending leaves which might enhance the carbon supply to the opposite bolls. [Conclusion] Lower temperature due to late planting could contribute to the more full development of cotton bolls. Late planting is an alternative consideration in improvement of fiber quality in cotton cultural practices.  相似文献   

8.
花铃期遮阴对棉花氮素代谢的影响及其机制研究   总被引：2，自引：0，他引：2  
为明确花铃期弱光对棉花氮素代谢的影响及其内在机理,以苏棉15和科棉1号为材料,在棉株第6~8果枝的第1~2果节开花时利用遮阴网遮阴, 令透光率分别为100%、80%和60%,研究遮阴条件下棉花根系吸收能力及棉株氮素代谢相关酶活性动态变化特征。结果表明,在0~50 d的遮阴期内,随透光率减弱, 根系吸收能力及棉株氮素代谢相关酶活性降低;遮阴15 d后氮素代谢相关酶活性变化最为显著,在透光率60%处理下, 苏棉15、科棉1号根系硝酸还原酶、谷氨酰胺合酶、谷氨酸合酶、谷氨酸脱氢酶活性分别下降25.1%、53.2%、39.1%和25.5%,主茎功能叶相应酶活性分别下降50.3%、24.0%、30.4%和18.9%。根系、主茎功能叶变化程度不同, 说明二者的氮素代谢机制对遮阴的反应存在一定差异：遮阴条件下根系氮素代谢能力下降的原因主要是谷氨酰胺合酶/谷氨酸合酶循环介导的氮素同化能力下降,其次为硝酸还原酶介导的氮素还原能力下降;主茎功能叶氮素代谢能力下降的原因主要是硝酸还原酶介导的氮素还原能力下降,其次为谷氨酰胺合酶/谷氨酸合酶循环介导的氮素同化能力下降。遮阴条件下棉花碳、氮代谢能力降低,最终导致棉株根系、主茎功能叶氮素含量和累积量降低,棉花单株铃数、铃重、籽棉产量亦随遮阴程度加深显著减少(P<0.05)。  相似文献   

9.
选择3类棉纤维比强度差异明显的品种,于2004—2005年在江苏南京（长江流域下游棉区）研究棉纤维加厚发育过程中主要生理特性的基因型差异及对纤维比强度的影响,为探索改善棉纤维比强度的生理调控途径提供理论依据。结果表明,高纤维比强度基因型（科棉1号）棉纤维中可溶性糖转化多,进入纤维次生壁加厚发育期的β-1,3-葡聚糖含量峰值高,纤维素合成关键酶（蔗糖合成酶和β-1,3-葡聚糖酶）活性增强快、峰值高,纤维素累积速率平缓且快速累积期长;而较低纤维比强度基因型（苏棉15和德夏棉1号）的棉纤维加厚发育生理特征与此相反;中等棉纤维比强度基因型（美棉33B）则介于上述两者之间。与纤维素生物合成相关的物质和关键酶活性变化的基因型差异是造成纤维素累积速率及纤维比强度差异的主要生理原因之一。此外,β-1,3-葡聚糖含量的剧增可作为棉纤维进入次生壁加厚发育阶段的一个重要特征。  相似文献   

10.
刘霞  尹燕枰  贺明荣  王振林 《作物学报》2006,32(7):1063-1070
通过测定旗叶和籽粒中磷酸蔗糖合成酶(SPS)、蔗糖合成酶(SS)、AGP焦磷酸化酶(AGPase)、UGP焦磷酸化酶(UPGase)、可溶性淀粉合成酶(SSS)和束缚态淀粉合成酶(GBSS)的活性,以及蔗糖含量,研究了播种期对藁城8901蔗糖代谢和淀粉合成相关酶活性变化及淀粉组分积累的影响。结果表明,早播和晚播均可降低籽粒直链淀粉积累量,但其支链淀粉积累量随播期的推迟逐渐升高;直链淀粉和支链淀粉积累的最适平均日照时数分别为9.06和11.07 h;支链淀粉积累的最佳日均气温为22.75℃,而直链淀粉积累对温度的适应范围较宽,日均气温在17~25℃之间时,直链淀粉含量与日均气温呈正相关关系。推迟播期使旗叶SPS和SS活性升高,而旗叶蔗糖积累量、籽粒SS、SPS活性和蔗糖积累量均显著降低;籽粒AGPase、SSS活性和支链淀粉积累量明显升高;而UGPase、花后14 d的GBSS活性和直链淀粉积累量显著降低。表明在小麦植株体内存在淀粉积累对蔗糖代谢的反馈调节作用,它主要是通过降低籽粒SPS活性而不是SS活性来实现的。由SS催化的蔗糖降解不是提供小麦籽粒淀粉积累所需底物的惟一途径。在小麦籽粒淀粉合成过程中,AGPase、SSS、UGPase和GBSS均起着重要的调节作用。植株本身可通过反馈抑制籽粒UGPase和某一时期的GBSS活性来调节籽粒中蔗糖向淀粉的转化以及淀粉组分的积累,以维持体内合理的直/支比例。  相似文献   

11.
棉花源库调节对铃叶光合产物运输分配的影响   总被引：14，自引：0，他引：14  
通过早打顶去边心和人工剪叶,研究了减库或减源对铃、叶光合产物运转分配的影响。结果表明,当棉铃发育受减库调节时,铃体积增大,铃重提高,而且对高品质棉科棉1号影响较大;花后40 d科棉1号叶片可溶性糖和淀粉含量分别比对照增加41.0%和4.1%,常规棉苏棉15分别比对照增加14.8%和77.3%;花后30日铃对位叶标记24 h后14C光合产物的输出,科棉1号增加17.6%,苏棉15增加6.8%;棉铃光合产物输入率,科棉1号比苏棉15增加3.6%;此外,铃柄韧皮部中全氮、可溶性糖增加;棉铃发育受叶源限制调节时,铃体积减小,铃重降低,而且对苏棉15影响大;叶片中可溶性糖和淀粉含量减少,全株去3/4叶处理花后40 d科棉1号分别比对照减少8.7%和28.4%;苏棉15分别减少23.6%和32.2%。叶片14C光合产物输出率和棉铃14C光合产物输入率均降低,其中又以苏棉15下降幅度大。铃柄韧皮部中全氮、可溶性糖也相应下降。因此,通过技术手段调节源库,可以促进高品质棉叶片光合产物向棉铃输送,进而提高铃重。  相似文献   

12.
The number of seeds and seed yield per plant were higher in chickpea crops raised from water and mannitol (4 %) primed seeds in comparison with the control non-primed crops. In primed plants, an enhanced acid invertase activity in the apical part of the main stem and the part immediately below it at 100 and 130 days after sowing (DAS) might result in an increased availability of hexoses to these plant parts. In addition, decreased acid invertase activity at the point of initiation of branches and in the internodes of stem observed in primed plants indicated restricted hydrolysis of sucrose during its transport through the stem, resulting in its more supply to the actively growing sinks. The activities of sucrose-cleaving enzymes, i.e. invertase and sucrose synthase (SS) in podwall of primed plants were higher at 110 DAS. At 140 DAS, a stage of rapid seed filling, increased activities of SS and sucrose phosphate synthase (SPS) were observed in seeds of primed plants. Increased SPS activity in seeds of primed crop could meet the increased assimilate requirements of the developing seeds. Higher activity of SS in seeds of primed crop may facilitate seed filling. These data suggest that enzymes of sucrose metabolism play an important role in increasing the yield of chickpea crops raised from primed seeds.  相似文献   

13.
高品质陆地棉棉铃发育特点   总被引：5，自引：1，他引：4  
陈源  王永慧  肖健  栾娜  张祥  陈德华 《作物学报》2010,36(8):1371-1376
以高品质棉杂交种科棉3号、常规种科棉4号为供试品种(常规品质棉苏棉[1]5为对照),于2004-2005年测定棉铃的发育性状。结果表明,高品质棉与常规品质棉明显不同,前者铃较长、铃体积大、铃重高,棉铃最大直径与后者相似。科棉3号以花后10 d内铃长增长快于常规品质棉、科棉4号则以花后10 d、21~30 d快于常规品质棉;高品质棉铃最大直径的增长持续期短于常规品质棉,且花后10 d内增长慢;高品质棉在花后20 d内铃体积的增大高于常规品质棉,尤其以花后11~20 d增长更快;科棉3号在开花后10 d后铃重增长快于常规品质棉,科棉4号则表现在开花后20 d前快于常规品质棉。说明以铃长为重要指标进行高品质类型棉花品种选育、以棉铃体积和铃重提高为载体进行高产优质栽培调节是关键技术。  相似文献   

14.
花生籽仁的含糖量与其食用品质和加工特性密切相关,为了明确花生籽仁发育过程中糖分积累特征以及与蔗糖代谢相关酶活性的关系,以3个不同蔗糖含量的花生品种为材料,分别比较了它们在籽仁发育不同时期可溶性总糖、蔗糖、果糖、葡萄糖含量以及蔗糖磷酸合酶(SPS)、蔗糖合酶(SS)、中性转化酶(NI)、酸性转化酶(SAI)等蔗糖代谢关键酶的活性差异。结果表明不同品种在籽仁发育过程中的糖组分含量及酶活性变化规律相似;无论是合成方向还是分解方向,都以SS活性值相对最高;相关性分析显示,籽仁发育后期蔗糖含量与NI呈极显著负相关,与SPS呈显著正相关。推测可以在花生籽仁趋于成熟时,通过调节NI和SPS活性来提高其蔗糖含量。  相似文献   

15.
为探讨光质对绿色棉纤维生长发育和黄酮合成的影响,以绿色棉品种绿絮棉1号为材料进行离体培养,比较红光、黄光、蓝紫光、白光和暗处理条件下绿色棉胚珠鲜物质质量、纤维长度、纤维素含量、黄酮含量等生理和分子指标的差异。结果表明:光处理能够促进胚珠鲜物质质量的增加,但抑制了纤维的伸长。在纤维发育前期,各光处理抑制了蔗糖合成酶活性和蔗糖合成酶基因表达;纤维发育后期,白光处理提高了蔗糖合成酶活性,促进了蔗糖合成酶基因表达。红光、黄光和蓝紫光处理显著提高了β-1,3￣葡聚糖酶活性(10~20 DAC(Days after culture));红光、黄光和白光均能提高β-1,3￣葡聚糖基因表达量,而蓝光则抑制了基因的表达。白光促进了纤维素的合成,而蓝紫光抑制了纤维素的合成。在纤维发育初期(10 DAC),蓝紫光抑制了黄酮合成及其关键酶基因表达;而在发育后期(30~40 DAC),白光处理能够促进黄酮的合成及其关键酶基因表达。可见,光质对绿色棉纤维发育及黄酮合成具有一定的影响。  相似文献   

16.
王文静  王国杰  王永华 《作物学报》2007,33(7):1122-1128
在池栽条件下,研究了藁城8901(强筋品种)、豫麦49(中筋品种)和洛麦1号(弱筋品种)小麦灌浆期旗叶、叶鞘、穗茎、籽粒蔗糖磷酸合酶(SPS)、蔗糖合酶(SS)活性变化及其与淀粉及组分积累的关系。结果表明,其SPS和SS活性变化均呈单峰曲线。豫麦49旗叶、旗叶鞘和籽粒中及洛麦1号穗茎中SPS酶活性较高。豫麦49的旗叶、旗叶鞘、穗茎和籽粒中SS活性高于其他两品种,表明豫麦49和洛麦1号营养器官和籽粒中蔗糖合成和降解代谢比较活跃。相关分析表明,豫麦49和洛麦1号籽粒中淀粉和支链淀粉积累对SPS催化的蔗糖合成过程具有较强的反馈调节作用。豫麦49和洛麦1号籽粒中SS活性均与总淀粉与支链淀粉积累速率极显著正相关,而在藁城8901中无此相关性。  相似文献   

17.
试验以新棉33B为材料,于2007年和2009年在江苏南京(32º02′N,118º50′E)江苏省农业科学院防雨棚的水泥池中进行。以正常灌水(土壤相对含水量为75%±5%)为对照,于棉花中部(6~8)果枝开花时进行渍水7 d和14 d (保持地面2~3 cm的水层)处理。研究花铃期渍水对棉铃对位叶蔗糖代谢及铃重形成的影响。结果表明,花铃期渍水条件下棉铃对位叶可溶性糖、蔗糖、淀粉含量增加,蔗糖/淀粉比值下降,且随渍水持续期的延长变化幅度增大,表明花铃期渍水致使叶片蔗糖外运受阻且随渍水时间的延长外运受阻加重;棉铃对位叶蔗糖磷酸合酶、蔗糖合酶和酸性转化酶活性增强,且随渍水持续期的延长和果枝部位的上升而增大;棉铃对位叶可溶性糖、蔗糖和淀粉含量与蔗糖磷酸合酶、蔗糖合酶及酸性转化酶活性显著正相关(P<0.05),表明渍水条件下蔗糖代谢被激活但并未促进叶片蔗糖的外运,铃重随果枝部位上升和渍水持续期的延长下降幅度增大。这是渍水条件下铃重下降的重要原因。  相似文献   

18.
为明确高温对甜玉米籽粒产量和品质的影响,以甜玉米厦甜1号和粤甜16为材料, 人工授粉3 d后, 利用人工气候室分别进行昼间高温(HT)35℃和正常气温(CK)处理, 研究了甜玉米籽粒灌浆过程中粒重、糖分积累和蔗糖代谢相关酶活性的动态变化。结果表明, 与正常气温相比, 高温缩短甜玉米灌浆进程, 显著降低粒重、含水量, 提高皮渣率。在最佳采收期(水分含量为68%~74%)甜玉米鲜百粒重分别下降了20.8% (厦甜1号)和16.4% (粤甜16)。在甜玉米籽粒灌浆过程中可溶性糖和蔗糖的含量随授粉后时间先升高后降低, 高温处理不利于可溶性糖和蔗糖积累, 籽粒中糖分含量降低, 淀粉含量升高。籽粒中蔗糖的合成与分解主要由磷酸蔗糖合成酶(SPS)和蔗糖合成酶(SS)催化, SS催化反应是可逆的。高温明显降低SPS和SS合成方向活性, 提高SS分解方向活性, 导致糖分含量降低, 品质变劣。高温缩短两品种SS(合成方向)活性峰值出现的时间, 而对于SPS活性峰值出现时间厦甜1号被缩短, 粤甜16 为不变。  相似文献   

19.
Waterlogging is a main factor causing rapeseed (Brassica napus L.) yield loss, and reasonable nitrogen (N) applications can compensate for this loss. To investigate the effects of N rates on seed yield of waterlogged rapeseed, the waterlogging-tolerant rapeseed variety ZS 9 and sensitive variety GH01 were waterlogged for 0 and 10 days with five leaves at the seedling stage under four N rates (0, 90, 180 and 270 kg/ha). Waterlogging significantly decreased seed yield, while N application can alleviate the yield loss. The yield decrease rate of waterlogged GH01 was greater than that of ZS 9 under the same N rate. During the seedling and bolting stage, the leaf photosynthetic rate (Pn) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity increased, while activities of adenosine diphosphate glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) decreased with more N under the same watering conditions. Compared to the plants without waterlogging, the leaf Pn and Rubisco activity, starch and sucrose contents of waterlogged rapeseed decreased at the two stages; activities of AGPase, SuSy and SPS of waterlogged rapeseed decreased at the seedling while increased at the bolting stage for both the two varieties. At the flowering stage, the Pn, the activities of Rubisco, AGPase, SuSy, SPS and contents of sucrose, starch increased with more N application for both ZS 9 and GH01. Compared to the plants without waterlogging, the Pn and Rubisco activity for waterlogged plants of the two varieties increased; the waterlogged plants of tolerant variety had higher activities of AGPase, SuSy and SPS, while those of sensitive variety was significantly lower. However, the decreased starch and sucrose content were found in both tolerant and sensitive varieties. The activities of AGPase, SuSy and SPS at flowering were highly positively correlated with yield under the interactive effects of N and waterlogging. These results suggested that the flowering stage is the most important stage that N had the positive regulation on waterlogged rapeseed growth. The carbohydrates translocation from leaves to seeds of the tolerant variety were enhanced after waterlogging, while that of the sensitive variety was still inhibited. This was the main reason for the difference in yield between the two waterlogged varieties.  相似文献   

20.
不同类型小麦品种灌浆期蔗糖代谢关键酶的活性变化   总被引：1，自引：0，他引：1  
测定池栽条件下小麦强筋品种藁城8901、中筋品种豫麦49号和弱筋品种洛麦1号灌浆期旗叶、旗叶鞘、穗茎、籽粒中蔗糖磷酸合成酶(SPS)、蔗糖合成酶(SS)活性变化的结果表明,3种不同类型小麦品种灌浆期旗叶、旗叶鞘、穗茎、籽粒中SPS和SS活性变化均呈单峰曲线。豫麦49号旗叶、旗叶鞘和籽粒中SPS酶活性较高,而洛麦1号穗茎中SPS酶活性较高。豫麦49号的旗叶、旗叶鞘、穗茎和籽粒中SS活性高于其他两品种,表明豫麦49号和洛麦1号营养器官和籽粒中蔗糖合成和降解代谢比较活跃。  相似文献   

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