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1.
Growth performance and food conversion efficiency (FCE) were investigated in juvenile spotted wolffish (Anarhichas minor Olafsen), mean (S.D.) initial weight 15.7 (4.8) g, reared at four levels of carbon dioxide (CO2(aq)) for 10 weeks at 6 °C and 33‰. CO2 levels averaged 1.1 (control), 18.1 (low), 33.5 (medium) and 59.4 (high) mg l−1, with corresponding pH values of 8.10, 6.98, 6.71 and 6.45, respectively. In addition, kidneys from sampled fish were examined macroscopically for gross signs of calcareous deposits, i.e. nephrocalcinosis, at the start and end of the experiment. Growth was significantly reduced at the highest concentration (P<0.0001), as compared to all other groups, while no overall differences in growth rate or mean weight were seen in the range of 1.1–33.5 mg CO2 l−1 at the end of the experiment. Daily feeding rates and total food consumption were reduced at the highest concentration (P<0.001), whereas food conversion efficiency did not vary significantly between groups. Plasma chloride levels displayed a significant decrease with increasing CO2 levels, from 151.3 mmol l−1 (1.1 mg CO2 l−1) to 128.3 mmol l−1 (59.4 mg CO2 l−1) at the end of the experiment, whereas plasma osmolality in the high CO2 group was significantly higher compared to the control group at the end of the experiment (371.4 and 350.8 mOsmol kg−1, respectively). Nephrocalcinosis was observed in all groups at the end of the experiment, but was most pronounced in the medium and high CO2 group.  相似文献   

2.
Several studies have shown that food ration can affect the growth of cultured fish. Determining the optimal food ration would help to achieve better growth and also provide direct economic benefits due to reduced food wastage, which would lead to commercial success. Therefore, we studied the effects of ration levels on growth performance of 0+ juvenile yellowtail flounder to determine the optimal food ration. Two experiments were conducted; the first experiment as a preliminary using ration levels of 1%, 2%, 4%, 6% body weight per day (% bw day−1) held at 7.0 °C with a stocking density of 0.95 kg m−2 (45% bottom coverage). Results of this preliminary experiment indicated that fish fed with 1% bw day−1 had significantly lower growth (weight, length, body depth and specific growth rates (SGR)) than those fed with 2%, 4% and 6% ration. However, fish fed with rations of 1% and 2% showed significantly lower gross food conversion ratios (GFCR) than fish fed with 4% and 6% rations. Survival was not significantly affected by different ration levels. Based on these preliminary results, we used ration levels of 1%, 1.5%, 2% and 3% for the main experiment. Fish were held at 10 °C with a stocking density of 1.45 kg m−2 (34% bottom coverage). Results indicated that fish fed with 1%, 1.5% and 2% bw day−1 had significantly lower growth than fish fed with 3% bw day−1. GFCR was significantly different for all four rations. It was lower for 1% than 1.5%, 2% and 3% rations. Survival was not significantly different between any treatments. We discuss our results with emphasis on growth and economics (i.e., feed wastage) and stress the need to balance both components in a commercial operation.  相似文献   

3.
A cyanobacterium (Spirulina platensis) was co-cultured with black tiger shrimp (Penaeus monodon) for water quality control. We evaluated the effects of: (1) three S. platensis trial conditions on inorganic nitrogen concentrations at one shrimp density (S. platensis trial conditions included: absent, nonharvested and semicontinuous harvesting) and (2) two shrimp densities on inorganic nitrogen concentrations, with and without S. platensis. Semicontinuous harvesting of S. platensis at one shrimp density resulted in significantly reduced (P<0.05) inorganic nitrogen concentrations (NH4, NO2 and NO3). With S. platensis absent, ammonium and nitrite concentrations ranged from 0.5 to 0.6 mg l−1, while nitrate concentrations ranged from 16 to 18 mg l−1 by day 44. With nonharvested S. platensis, considerable variability occurred with nitrogen concentrations. Semicontinuous harvest of S. platensis reduced nitrate to 4 mg l−1, while ammonium and nitrite ranged from 0.0 to 0.15 mg l−1, respectively. The factorial evaluation of shrimp density versus presence and absence of S. platensis resulted in greatly reduced nitrogenous compounds with S. platensis present regardless of shrimp density, and only moderately increased nitrogen with greater shrimp density. Without S. platensis, all nitrogen compounds were substantially elevated and shrimp survived was significantly reduced at high shrimp density.  相似文献   

4.
The culture of the mulloway (Argyrosomus japonicus), like many other Sciaenidae fishes, is rapidly growing. However there is no information on their metabolic physiology. In this study, the effects of various hypoxia levels on the swimming performance and metabolic scope of juvenile mulloway (0.34 ± 0.01 kg, mean ± SE, n = 30) was investigated (water temperature = 22 °C). In normoxic conditions (dissolved oxygen = 6.85 mg l− 1), mulloway oxygen consumption rate (M·o2) increased exponentially with swimming speed to a maximum velocity (Ucrit) of 1.7 ± < 0.1 body lengths s− 1 (BL s− 1) (n = 6). Mulloway standard metabolic rate (SMR) was typical for non-tuna fishes (73 ± 8 mg kg− 1 h− 1) and they had a moderate scope for aerobic metabolism (5 times the SMR). Mulloway minimum gross cost of transport (GCOTmin, 0.14 ± 0.01 mg kg− 1 m− 1) and optimum swimming velocity (Uopt, 1.3 ± 0.2 BL s− 1) were comparable to many other body and caudal fin swimming fish species. Energy expenditure was minimum when swimming between 0.3 and 0.5 BL s− 1. The critical dissolved oxygen level was 1.80 mg l− 1 for mulloway swimming at 0.9 BL s− 1. This reveals that mulloway are well adapted to hypoxia, which is probably adaptive from their natural early life history within estuaries. In all levels of hypoxia (75% saturation = 5.23, 50% = 3.64, and 25% = 1 .86 mg l− 1), M·o2 increased linearly with swimming speed and active metabolic rate (AMR) was reduced (218 ± 17, 202 ± 14 and 175 ± 10 mg kg− 1 h− 1 for 75%, 50% and 25% saturation respectively). However, Ucrit was only reduced at 50% and 25% saturation (1.4 ± < 0.1 and 1.4 ± < 0.1 BL s− 1 respectively). This demonstrates that although the metabolic capacity of mulloway is reduced in mild hypoxia (75% saturation) they are able to compensate to maintain swimming performance. GCOTmin (0.09 ± 0.01 mg kg− 1 m− 1) and Uopt (0.8 ± 0.1 BL s− 1) were significantly reduced at 25% dissolved oxygen saturation. As mulloway metabolic scope was significantly reduced at all hypoxia levels, it suggests that even mild hypoxia may reduce growth productivity.  相似文献   

5.
Juvenile greenlip abalone, Haliotis laevigata, (mean whole weight 4.48±1.9 g, mean±s.d., n=953) were highly sensitive to ammonia as indicated by depressed growth rate and food consumption measured over 2–3 months in bioassay tanks. For growth rate expressed on a whole weight basis, the EC5 and EC50 values (5 and 50% growth reductions) were 0.041 mg FAN l−1 (Free Ammonia–Nitrogen) and 0.158 mg FAN l−1, respectively. Shell growth rates declined over the entire experimental range (0.006–0.188 mg FAN l−1). At the end of the bioassay, groups of abalone were transferred to respiratory chambers. Oxygen consumption rate increased to a maximum of 188% of control values at 0.235 mg FAN l−1 and decreased slightly at the highest concentration of 0.418 mg FAN l−1.  相似文献   

6.
In order to develop a simple and accurate index of the salinity resistance of tilapia, batches of 10 juveniles (5 to 20 g) of two different species Oreochromis niloticus and Sarotherodon melanotheron reared in freshwater were subjected to gradual increases in salinity until 100% mortality. Seven daily increments of salinity were tested with 4 replicates: 2, 4, 6, 8, 10, 12 and 14 g l−1 day−1, while control batches were kept in fresh water. The temperature was maintained at 27 °C. The concentration of oxygen, ammonia and the pH were not limiting factors. The mortality, monitored on a daily basis, appeared after 2–51 days and was spread out over 1–20 days, depending on the increment of salinity. The higher the daily rate in salinity increase, then the shorter the time lapse before total mortality occurred. The cumulative mortality as a function of salinity fit well with simple linear regressions. The criterion of the resistance to salinity was the index MLS (median lethal salinity) defined at each daily rate as the salinity at which 50% of fish died. For S. melanotheron, the mean MLS was 123.7±3.5 g l−1 whatever the daily rate in salinity. For O. niloticus, the MLS was 46.3±3.4 g l−1 for daily increases in salinity ranging from 2 to 8 g l−1 day−1 and decreased significantly (P<0.05) above this level. The MLS-8 g l−1 day−1 ,which takes into account the full capacity of the fish to adapt to the increasing salinity, appeared to be a simple, optimized and efficient criterion for assessing the resistance to salinity for O. niloticus and S. melanotheron. This criterion can be a useful tool for ranking the different parental strains and hybrids of different genus and species of tilapia used in programmes of genetic selection for growth and salinity tolerance.  相似文献   

7.
This study was carried out for three successive years during 1999–2001 to evaluate growth and yield performance of fish, prawn and paddy under rice–fish integration system in rainfed medium land ecosystem. Irrespective of stocking density, faster growth rate was recorded for Catla catla followed by Cyprinus carpio, Cirrhinus mrigala, Labeo rohita and Macrobrachium rosenbergii. C. carpio and C. mrigala performed better growth rate against that of L. rohita probably due to the fact that being bottom dwellers, C. carpio and C. mrigala are more tolerant to fluctuation of oxygen concentration. Productivity of fish and prawn was, however, higher (p<0.05) in refuges with 10-cm weir height plots, irrespective of stocking density, while overall yield performance was good at stocking density of 25,000 ha−1. It was observed that, even with supplemental feeding, with increase in stocking density, biomass yield increased up to an optimum and then decreased. An average minimum and maximum yield of 906.6–1282.3 kg ha−1 of fish and prawn has been achieved, which was much higher than the earlier recorded productivity in a season under rice–fish integration system. Highest grain yield was recorded at 15-cm weir height plot (3629 kg ha−1), probably contributed by higher number of panicles per square meter (235.5) and number of filled grains per panicle (121.7). Percentage increase in rice yield under rice–fish integration system was 7.9–8.6% against control, where paddy was cultivated without integration of fish and prawn probably due to better aeration of water, greater tillering effect and additional supply of fertilizer in form of leftover feed and fish excreta. Irrespective of stocking density, the overall rice equivalent yield (REY) of the system was high (4.22–4.55 tons ha−1) at 12.5-cm weir height plots-cum-refuge, without using any pesticide, herbicide, etc.  相似文献   

8.
The 96-h LC50 of ammonia-N and the effects of dietary vitamin C on oxygen consumption, ammonia-N excretion and Na+/K+ ATPase activity of Macrobrachium nipponense exposed to ambient ammonia were investigated. The results showed that the 96-h LC50 of ammonia-N was 36.6 mg l−1 for the freshwater prawn, M. nipponense, at pH 8.0. When prawns were exposed to high ambient ammonia-N concentrations, the oxygen consumption rate increased and ammonia excretion decreased. Dietary vitamin C supplementation led to higher oxygen consumption and lower ammonia excretion. Na+/K+ ATPase activity increased with increased ambient ammonia-N exposure in the range of 0–18.3 mg l−1, and then was reduced at ambient ammonia-N 36.6 mg l−1. Na+/K+ ATPase activities of prawns fed a vitamin C-supplemented diet were significantly lower than those of prawns fed a diet which was not supplemented with vitamin C.  相似文献   

9.
Abstract. A preliminary study was conducted to assess the performance of the tilapia, Oreochromis spilurus (Günther), cultured in seawater cages at different stocking densities during the nursing and rearing phases. The stocking densities tested were 200, 400 and 600 fish/m3 during the nursing phase and 100, 200, and 300 fish/m3 during the rearing phase.
In both growth phases, no significant differences (P>0.05) were observed among the different stocking densities on the mean individual final weight, daily growth rate, feed conversion ratio and survival rate. Yields in cages stocked with 400 and 600 fish/m3 during the nursing phase, however, were significantly (P<0.05) higher compared with cages stocked with 200 fish/m3. No significant differences (P>0.05) were observed between 400 and 600 fish/m3. A density of 600/m3 is therefore considered to be optimum for the nursing phase.
Yields of cages during the rearing phase increased significantly (P<0.01) with the increase in stocking density. After grading the fish, however, no significant differences (P>0.05) were observed when only fish bigger than 150g were considered. The occurrence of exophthalmia (cataract) was observed in two of the four replicates at the highest stocking density (300 fish/m3) during the rearing stage. Therefore, a stocking density of 200 fish/m3 is considered optimum for the rearing phase.  相似文献   

10.
Filtration rates of hatchery-reared king scallop (Pecten maximus L.) juveniles, fed a single species alga diet (Pavlova lutheri (Droop) Green), were measured at a range of temperatures (6–21 °C). Weight specific filtration rate (ml min−1 g−1 (live weight)) of juveniles of a selected size range of 17–19 mm shell height (0.26–0.36 g live weight) increased with temperature above 16 °C and decreased below 11 °C, but was not significantly different between these two temperatures. Measurements at 16 °C using juveniles with a wider size range of 10–25 mm shell height (0.05–0.8 g live weight) gave the allometric equation: filtration rate (ml min−1)=12.19×weight (g)0.887. Filtration rate decreased significantly when the cell concentration was greater than 200 cells μl−1 (4.25 mg (organic weight) l−1). With six other algae food species, filtration rates similar to those with P. lutheri were only achieved with Chaetoceros calcitrans (Paulsen) Takano. All other algae species tested were cleared from suspension at significantly lower rates. Experiments with diet mixtures of P. lutheri and these other algae suggested that this was usually a reflection of lowered filtration activity, rather than pre-ingestive rejection of cells. In experimental outdoor nursery rearing systems, the filtration rate was inversely proportional to the concentration of cells in the inflow, in the range 5–210 cells μl−1. It was not affected by flow rate (2–130 l h−1, equivalent to 0.12–28.38 l h−1 g−1 (live weight)) with scallop juveniles stocked from 2 to 62 g l−1. The results are discussed in relation to on-growing scallops at field sites.  相似文献   

11.
Hizikia fusiforme (Harv.) Okamura (brown seaweed) was cultured using aeration with two CO2 conditions: outdoor air (actual atmospheric CO2 concentration, averaging 360 μl l− 1) and CO2-enriched air (averaging 700 μl l− 1), to investigate the possible adjustments of elevated atmospheric CO2 to the growth, photosynthesis and nitrogen metabolism in this mariculture species. Aeration with CO2-enriched air reduced the pH in the culture medium in comparison with aeration with air. The mean relative growth rate was enhanced when H. fusiforme was grown at high CO2 with respect to normal CO2. There was little change in the rate of light-saturated photosynthesis, dark respiratory rate and apparent photosynthetic efficiency, measured in natural seawater, between thalli grown in high and normal CO2 contents. However, both the mean nitrate uptake rate and the activity of nitrate reductase at light period were increased following culture at high CO2, indicating an enhanced nitrogen assimilation of H. fusiforme thalli with the CO2 enrichment in culture. It was proposed that the intensive cultivation of H. fusiforme would remove nutrients more efficiently with the future elevation of CO2 levels in seawater, which could be a possible solution to the problem of ongoing coastal eutrophication.  相似文献   

12.
A hydraulically integrated serial turbidostat algal reactor (HISTAR) for the mass production of microalgae was designed, constructed and preliminarily evaluated. The 9266-l experimental system consists of two enclosed turbidostats hydraulically linked to a series of six open continuous-flow, stirred-tank reactors (CFSTRs). The system was monitored and controlled using GENESIS process control software. A production study was preformed using Isochrysis sp. (C-iso) to assess system stability and production potential under commercial-like conditions. The study was performed at the following target system parameters: system dilution rate of 0.49 per day, pH 7.6, NITROGEN=10 mg l−1, PHOSPHORUS=2 mg l−1, and artificial illumination (photosynthetic photon flux density) from 1000 W metal halide LAMPS=800 μmol s−1 m−2. At steady state conditions, daily harvested algal paste was 1454 g (wet), mean areal system PRODUCTIVITY=47.8±3.04 g m−2 per day (17.1±1.09 g C m−2 per day) and mean CFSTR6 DENSITY=105.5±6.71 mg l−1.  相似文献   

13.
Abstract. Six groups of tropical freshwater catfish, Mystus nemurus (Cuvier & Valenciennes)(mean weight. 20·45 ± l·5g), were reared in 0·34m3 fibreglass tanks at different stocking densities (105, 195, 285, 375, 465 and 555 specimens/m3 water) for 84 days. The objective of the study was to determine the effect of various stocking densities on the growth, nutrition, biochemical composition and survival of M. nemurus. The lowest growth rate appeared in fish at the highest density and the highest was observed in fish stocked in moderate density of 285 and 375 fish/m3 water. Fish production was also lower at relatively low stocking densities of 105 and 195 fish/m3. Food conversion ratio (FCR), protein efficiency ratio (PER) and biochemical composition of M. nemurus indicate that there exists an optimum stocking density which lies between 285 and 375 fish/m3.  相似文献   

14.
The growth and survival of three size classes of wild caught western rock lobster, Panulirus cygnus (post-pueruli: mean 2.14 ± 0.07 g, 13.2 ± 0.1 mm CL; year 1: post-settlement juveniles, 57.1 ± 1.1 g, 38.7 ± 0.28 mm CL; and year 2 post-settlement juveniles, mean 138.2 ± 2.26 g, 51.9 ± 0.25 mm CL) were examined at combinations of two stocking densities (post-pueruli: 50 and 100 m− 2; year 1: 11 and 23 m− 2; year 2: 10 and 19 m− 2) and two shelter types (a novel rigid plastic mesh shelter or bricks) over a period of 6 months. Survival of lobsters held at the lower densities (90–95%) was significantly greater than for lobsters held at higher densities (post-pueruli = 78%, year 1 = 86%, year 2 = 88%). Post-pueruli survival was significantly higher in tanks with mesh shelters (91.7%) than brick shelters (75.8%) with a similar trend exhibited by year 1 and year 2 lobsters. Densities tested did not significantly affect lobster growth for any size class. Growth of post-pueruli was considerably higher in tanks with mesh shelters (641.7% weight gain; specific growth rate 1.07 BW day− 1) (p < 0.05) but there was no difference in the growth of year 1 and year 2 lobsters between mesh and brick shelters. Feed intake (g pellet dry matter lobster− 1 day− 1) was not significantly different between densities. This study has shown that P. cygnus is well suited for aquaculture based on the collection and ongrowing of wild caught pueruli, as this species exhibits good survival at high densities (up to 100 m− 2) without adverse effects on growth, and shows no captivity-related health problems. We recommend mesh shelters, with stocking densities of 50 m− 2 for post-pueruli and between 20 and 25 m− 2 for year 1 and year 2 juveniles, to maximise survival and production.  相似文献   

15.
Abstract.
The study was conducted to develop guidelines for high-density overwintering of tilapia in tanks using warm underground sea water. Seawater-acclimated fish of 20 g were stocked in 36 tanks at 250, 500 and 750/m3. Water flow was regulated at 0.1 and 0.2 l/kg fish/min. Fish were fed at the rates of 0.75% and 1.0% of biomass per day.
After 135 culture days, the mean individual weight gain and specific growth rate decreased, whereas feed conversion increased significantly ( P <0.0001) with the increase in stocking density. The condition factor at 500 and 750 fish/m3 was significantly lower ( P <0.0005) than at 250 fish/m3. However, stocking density had no significant effect on the survival rate. Significantly better specific growth rate, condition factor and feed conversion were observed at a water flow rate of 0.2 l/kg fish/min than at 01 l/kg fish/min. Significantly higher mean individual weight gain, specific growth rate, and survival rate were observed at 1.0%/day than at the 0.75%/day feeding rate. The findings indicate that the optimum stocking density for overwintering tilapia in tanks using warm underground sea water is 750 fish/m3 with a water flow rate of 0.1 l/kg fish/min and a feeding rate of 0.75%/day.  相似文献   

16.
Behavioral and ventilatory parameters have the possibility of predicting the stress state of fish in vivo and in situ. This paper presents a new image-processing algorithm for quantifying the average swimming speed of a fish school in an aquarium. This method is based on the alteration in projected area caused by the movement of individual fish during frame sequences captured at given time intervals. The image enhancement method increases the contrast between fish and background, and is thus suitable for use in turbid aquaculture water. Behavioral parameters (swimming activity and distribution parameters) and changes in ventilation frequency (VF) of tilapia (Oreochromis niloticus) responded to acute fluctuations in dissolved oxygen (DO) which were monitored continuously in the course of normoxia, falling DO level, maintenance of hypoxia (three levels of 1.5, 0.8 and 0.3 mg l−1) and subsequent recovery to normoxia. These parameters responded sensitively to acute variations in DO level; they displayed significant changes (P < 0.05) during severe hypoxia (0.8 and 0.3 mg l−1 level) compared with normoxic condition, but there was no significant difference under conditions of mild hypoxia (1.5 mg l−1 level). There was no significant difference in VF between two levels of severe hypoxia 0.8 and 0.3 mg l−1 level during the low DO condition. The activity and distribution parameters displayed distinguishable differences between the 0.8 and 0.3 mg l−1 levels. The behavioral parameters are thus capable of distinguishing between different degrees of severe hypoxia, though there were relatively large fluctuations.  相似文献   

17.
Profiles of changes in physiological parameters of freshwater giant prawns, Macrobrachium rosenbergii, exposed to various dissolved oxygen (DO) levels of 7.75, 4.75, 2.75, and 1.75 mg l−1 are reported. The parameters involved in osmoregulation and oxygen transport were monitored for a 6-day period. Notable depressions in hemolymph osmolality, Na+, K+, and Cl− contents were observed within 24 h after exposure to hypoxia at 2.75 and 1.75 mg O2 l−1, and thereafter remained at rather steady levels, which were significantly lower than those under normoxic conditions (4.75–7.75 mg O2 l−1). The extent of depression of osmotic-related constituents, hemolymph osmolality and Cl− in particular, increased with decreased DO.

Oxyhemocyanin constituted 65.46–65.84% of total hemolymph proteins under the various DO levels examined; both hemolymph oxyhemocyanin and proteins showed notable elevations 24 h after exposure to hypoxic conditions, and reached the highest and constant level by 48 h after exposure. The compensatory responses of prawns to reduced O2 were manifested by increased O2 uptake through augmentation of hemocyanin, which results in enhancement of oxygen binding capacity of the hemolymph. In the same period, a significant surge of the respiratory products, PCO2 and HCO3−, was also demonstrated 6 h after exposure to hypoxic conditions which resulted in hemolymph alkalosis. These processes likely resulted in an increase in water influx and consequent declines in hemolymph osmolality and ion composition. Furthermore, hyperventilation and respiratory alkalosis, indicated by increased oxyhemocyanin and pH, respectively, were found to be predominant responses of M. rosenbergii to hypoxic stress.  相似文献   


18.
Antifungal agents are essential for the maintenance of healthy stocks of fish and their eggs in intensive aquaculture operations. In the USA, formalin is the only fungicide approved for use in fish culture. However, hydrogen peroxide and sodium chloride have been granted low regulatory priority drug status by the United States Food and Drug Administration (FDA) and their use is allowed. We evaluated the efficacy of these fungicides for controlling fungal infections on rainbow trout eggs. A pilot study was conducted to determine the minimum water flow rate required to administer test chemicals accurately in Heath incubators. A minimum water flow rate of 7.6 1 min−1 was necessary to maintain treatment concentrations during flow-through chemical exposures. The antifungal activity of formalin, hydrogen peroxide, and sodium chloride was evaluated by treating uninfected and 10% fungal-infected (Saprolegnia parasitica) rainbow trout eggs (Oncorhynchus mykiss) for 15 min every other day until hatch. There were no significant differences among treatments in percent hatch or final infection for uninfected eggs receiving prophylactic chemical treatments. Eggs of the negative control group (uninfected and untreated) had a mean hatch exceeding 86%. All chemical treatments conducted on the infected egg groups controlled the spread of fungus and improved hatching success compared with the positive control groups (infected and untreated). Formalin treatments of 1000 and 1500 μl l−1 and hydrogen peroxide treatments of 500 and 1000 μl l−1 were the most effective. Sodium chloride treatments of 30 000 mg l−1 improved fry hatch, but the compound was less effective at inhibiting fungal growths compared with hydrogen peroxide and formalin treatments.  相似文献   

19.
A series of experiments were conducted to evaluate the effects of diet, stocking density and environmental factors on the growth, survival and metamorphosis of short neck clam Paphia malabarica larvae. These experiments examined the following factors: diet [ Isochrysis galbana , Nannochloropsis salina and a mixture of I. galbana and N. salina (1:1 w/w)], stocking density (1, 3, 5 and 7 larvae mL−1), light intensity (unshaded, partially shaded and fully shaded) and water filtration (unfiltered and sand filtered). Results indicated that N. salina could replace 50% of I. galbana as a food source for the clam larvae with an increase in growth, survival (47.2%), metamorphosis (33.5%) and early settlement. Larval growth decreased significantly with increasing stocking density. A density of 1–3 larvae mL−1 appeared to be optimal for normal growth of clam larvae. Neither diet nor stocking density used in the study had a significant effect on larval survival. Under partially shaded (light intensity=1000–5000 lx) and fully shaded (light intensity <1000 lx) conditions, larval growth was significantly faster than under direct sunlight (unshaded). Larvae grew significantly faster in the unfiltered water than in the filtered water.  相似文献   

20.
Abstract. A bioenergetic growth model was developed to examine the integrated effects of fertilization, stocking density, and spawning on the growth of tilapia, Oreochromis niloticus (L.), in pond aquaculture. The analyses showed that growth rates increase with higher levels of organic fertilization up to 500kg/ha/week. Growth rates increased with added food rations in ponds, reaching a maximum growth of 2-07g/day at about 44–48 days after stocking. Fish growth rates decreased with increased levels of stocking density. The stocking density for optimal growth is 1fish/m2; the optimal density for total harvesting weight and fish size is 2 fish/m2. Model sensitivity analysis indicated that tilapia growth is most sensitive to catabolism (metabolism) and anabolism (synthesis) coefficients, both of which are geometrically related to the fish body weight. Food assimilation efficiency (b) and the food consumption coefficient (h) have a modest effect on fish growth. Spawning in grow-out ponds can have a major effect on fish growth.  相似文献   

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