共查询到20条相似文献，搜索用时 78 毫秒
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蜕皮是甲壳动物重要的生理活动,与其蜕皮激素的合成密切相关,细胞色素P450(CYP)302a1是甲壳动物蜕皮激素合成通路中的关键酶之一。本研究克隆了罗氏沼虾CYP302a1基因(Mr-CYP302a1),cDNA全长1859 bp,开放阅读框(ORF)为1629 bp,编码543个氨基酸(aa),分子量大小为61.09 ku,等电点为8.42。氨基酸序列分析显示CYP302a1基因的保守结构域含有5个P450基因家族特征保守区域:heme-binding、helix-K、helixC、helix-I及PERF。系统进化分析结果显示Mr-CYP302a1首先与绿虾CYP302a1聚为一支,然后与凡纳滨对虾及三疣梭子蟹等十足目甲壳动物的CYP302a1聚为一支,与甲壳动物的亲缘关系最近。实时荧光定量PCR(qRT-PCR)检测表明Mr-CYP302a1在罗氏沼虾的多个组织中均有表达,其中在Y器官中的表达量最高,性腺中次之。同时研究发现,MrCYP302a1基因在罗氏沼虾的蜕皮后期(A期和B期)表达量很低,蜕皮间期(C期)表达量开始上升,在蜕皮前期D1亚期达到峰值。对Mr-CYP302a1进行蛋白表达及多克隆抗体制备,蛋白印迹法(Western blot,WB)检测表明Mr-CYP302a1蛋白在罗氏沼虾Y器官中的表达量最高,在蜕皮过程中的蜕皮前期D1亚期达到峰值。综上所述,该基因在罗氏沼虾的蜕皮过程中扮演着十分重要的角色。 相似文献
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Wnt1诱导分泌蛋白(WISP)基因家族与CYR61、CTGF、NOV基因共同构成了CCN家族。研究通过鲤基因组序列与斑马鱼WISP基因编码区全序列的比对获得WISP1a、WISP1b、WISP2、和WISP3等4条序列。经过克隆、测序、比对拼接得到其开放阅读框,分别是1 089、1 077、1 038和1 026 bp,它们都是由5个外显子和4个内含子构成,分别编码362、358、345和341个氨基酸。分子系统学分析表明,鲤4个WISP基因具有高度同源性,其中WISP1a、WISP1b和WISP2均含有4个模块,WISP3缺少第2个模块。通过RT-PCR检测WISP基因在鲤组织中的表达,结果表明,WISP1a鲤在13个组织中均有表达,皮肤中表达最高,脾、肠、卵巢次之,其他组织中表达较低。WISP1b在精巢、脑、皮肤、卵巢中表达较高。WISP2在血液、鳃中表达较高。WISP3在血液、脑、肝中表达较高。实时荧光定量PCR法分析WISP基因在鲤胚胎发育时期的表达,结果表明,除WISP3外,WISP1a、WISP1b和WISP2在前期表达较高,36 h表达最低,随后逐渐升高至第6天开始下降。 相似文献
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Hui Yang Meng Zhang Tongwei Ji Yingying Zhang Wenzhi Wei Qiuning Liu 《Aquaculture Research》2022,53(1):255-264
To identify the aetiology of the disease outbreak in cultured Procambarus clarkii, a dominant bacteria strain from the diseased crayfish hepatopancreas was isolated. It was identified as Aeromonas veronii based on biochemical identification and 16S rDNA sequencing. The symptoms of artificially infected crayfish were similar to those of naturally infected P. clarkii. The A. veronii was sensitive to norfloxacin, ciprofloxacin, ceftazidime, cefuroxime and ceftriaxone antibiotics, while resistant to penicillin, ampicillin, oxacillin and piperacillin. Virulence genes such as the enterotoxin genes (act, alt and ast) and haemolytic toxin genes (hlyA and aerA) were detected. After A. veronii infection, the connections between some liver tubules disappeared, the vacuoles appeared in the brush border and the mitochondria were enlarged. The antagonistic action of previous identified B. subtilis CK3 against A. veronii was also detected. The supernatant of B. subtilis CK3 exhibits a significant bactericidal effect on A. veronii. After B. subtilis CK3 immersion, the antioxidant enzymes and immune-related enzyme activities in hepatopancreas were significantly higher than control. The accumulative mortality caused by infection of A. veronii can be significantly reduced by adding B. subtilis CK3 into the aquatic water. These results demonstrated that B. subtilis CK3 could act as a water additive to improve the immune response of P. clarkii against newly identified A. veronii. The present study will provide a new way for the prevention and control of crayfish bacterial diseases and also provide technical support for the healthy cultivation of P. clarkii. 相似文献
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Chung-Saint Lin Tser-Sheng Lin Din-Yuan Yu Yi-Cheng Su Yung-Hsiang Tsai 《Journal Of Aquatic Food Product Technology》2013,22(8):1301-1310
ABSTRACTVibrio parahaemolyticus is a human pathogen frequently found in seafood. Once the seafood is contaminated by V. parahaemolyticus, it can become a vehicle for foodborne illness. The conventional culture methods for detection of V. parahaemolyticus are time-consuming and cannot differentiate pathogenic strains from nonpathogenic ones. In this study, a multiplex polymerase chain reaction (PCR) technique was investigated for detecting tdh, chiA, and toxR of V. parahaemolyticus. The sensitivity of the multiplex PCR was determined by testing 28 strains of V. parahaemolyticus, 15 non-V. parahaemolyticus strains, and fresh seafood spiked with cells of V. parahaemolyticus. All the strains were analyzed for production of thermostable direct hemolysin (TDH) and chitinase. This study showed that both the chiA and toxR are excellent markers for detecting V. parahaemolyticus strains, and a multiplex PCR targeting chiA and tdh genes can be applied to simultaneously detect environmental and pathogenic V. parahaemolyticus. 相似文献
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Nagwan El‐Habashi Sabreen E. Fadl Hanaa F. Farag Doaa M. Gad Abdelgawad Y. Elsadany Mohamed S. El Gohary 《Aquaculture Research》2019,50(10):2769-2781
The present trial was performed to evaluate the effect of dietary incorporation of dried Spirulina, Chlorella and their mixture on the immune status of Nile tilapia (Oreochromis niloticus) before and after artificial infection with pathogenic bacterium Aeromonas hydrophila (A. hydrophila). Two hundred and forty fish were divided into four groups: (a) a control group fed on a basal diet only, (b) a second group fed on a diet containing Spirulina 15%, (c) a third group fed on a diet containing Chlorella 15%, and (d) a fourth group fed on a diet containing a mixture of both Spirulina 15% and Chlorella 15%. At the end of the experiment (9 weeks), the four groups were experimentally infected with A. hydrophila for 7 days. Antioxidant enzymes, lysozyme and bactericidal activities and histopathological changes were determined just before the challenge test and 7 days post‐challenge. Significant (p ≤ 0.05) increases in fish body protein% before the challenge test and increases in serum antioxidant enzymes, lysozyme and bactericidal activity in the Chlorella and algal mixture groups before and after the challenge test were observed. Spirulina, Chlorella and their mixture groups significantly decreased serum malondialdehyde compared to the control group before and after the challenge test. Using Spirulina, Chlorella and their mixture mitigated the necrotic and degenerative changes induced by A. hydrophila and revealed well‐developed and multiple melanomacrophage centres. Thus, dietary Spirulina, Chlorella and their mixture inclusion in Nile tilapia fish proved to have a protective effect against A. hydrophila infection. 相似文献
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Elongate plasmodia with myxosporean spores belonging to the genus Unicapsula, Davis, 1924 were found in the skeletal muscle of the striped seabream, Lithognathus mormyrus (L.), a candidate for the mediterranean aquaculture. The only species of Unicapsula described from the Mediterranean is Unicapsula pflugfelderi Schubert et al. 1975, which occurs in the picarel, Spicara smaris (L.). For morphological and molecular comparison of U. pflugfelderi from S. smaris with Unicapsula sp. from L. mormyrus measurements of plasmodia and spores, ultrastructural details and 18S and 28S rDNA sequences were analysed. Whereas plasmodia were 2–3 times larger in S. smaris than in L. mormyrus (length 2.47–0.81 mm; width 0.22–0.09 mm; P = 0.000), spore morphology showed minor differences and both 18S and 28S rDNA sequences were 100% identical identifying the myxozoan as U. pflugfelderi. Scanning electron microscopy of the spores revealed a different shell valve distribution than the one used for the diagnosis of the genus Unicapsula. This resulted in a review of the genus Unicapsula dividing it into two morphological groups of different spore valve arrangement. TEM revealed the presence of a yet undescribed crystalline structure in the sporoplasm of the spores. 相似文献
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Comparative pathogenicity of Vibrio spp., Photobacterium damselae ssp. damselae and five isolates of Aeromonas salmonicida ssp. achromogenes in juvenile Atlantic halibut (Hippoglossus hippoglossus) 
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下载免费PDF全文 Juvenile Atlantic halibut (~100 mg, Hippoglossus hippoglossus) were exposed to Vibrio proteolyticus, a Vibrio spp. isolate, Photobacterium damselae ssp. damselae and five different isolates of Aeromonas salmonicida ssp. achromogenes via an hour‐long bath immersion to ascertain their variation in pathogenicity to this fish species. Results were analysed using Kaplan–Meier survival analysis. Analysis of the data from challenges using A. salmonicida ssp. achromogenes revealed three survival values of zero and a spread of values from 0 to 28.43. Challenges using a Vibrio spp isolate, V. proteolyticus and P. damselae resulted in Kaplan–Meier survival estimates of 31.21, 50.41 and 57.21, respectively. As all bacterial species tested could induce juvenile halibut mortalities, they must all be considered as potential pathogens. However, the degree of pathogenicity of A. salmonicida is isolate dependent. 相似文献
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Construction and evaluation of type III secretion system mutants of the catfish pathogen Edwardsiella piscicida 下载免费PDF全文
下载免费PDF全文 A Edrees H Abdelhamed S W Nho S B Park A Karsi F W Austin M Essa T Pechan M L Lawrence 《Journal of fish diseases》2018,41(5):805-816
Catfish is the largest aquaculture industry in the United States. Edwardsiellosis is considered one of the most significant problems affecting this industry. Edwardsiella piscicida is a newly described species within the genus Edwardsiella, and it was previously classified as Edwardsiella tarda. It causes gastrointestinal septicaemia, primarily in summer months, in farmed channel catfish in the south‐eastern United States. In the current study, we adapted gene deletion methods used for Edwardsiella to E. piscicida strain C07‐087, which was isolated from a disease outbreak in a catfish production pond. Four genes encoding structural proteins in the type III secretion system (T3SS) apparatus of E. piscicida were deleted by homologous recombination and allelic exchange to produce in‐frame deletion mutants (EpΔssaV, EpΔesaM, EpΔyscR and EpΔescT). The mutants were phenotypically characterized, and virulence and vaccine efficacy were evaluated. Three of the mutants, EpΔssaV, EpΔyscR and EpΔesaM, were significantly attenuated compared to the parent strain (p < .05), but EpΔescT strain was not. Vaccination of catfish with the four mutant strains (EpΔssaV, EpΔesaM, EpΔyscR and EpΔescT) provided significant protection when subsequently challenged with wild‐type strain. In conclusion, we report methods for gene deletion in E. piscicida and development of vaccine candidates derived from a virulent catfish isolate. 相似文献
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Molecular isolation and characterization of a haemocyanin of Macrobrachium rosenbergii reveal its antibacterial activities 下载免费PDF全文
下载免费PDF全文 Chutima Srisuk Saengchan Senapin William G Bendena Siwaporn Longyant Paisarn Sithigorngul Parin Chaivisuthangkura 《Aquaculture Research》2018,49(1):505-516
Haemocyanin is a multi‐subunit protein complex found in the haemolymph and is involved in the immune system of crustaceans. In this study, a haemocyanin gene of Macrobrachium rosenbergii, designated MrHc, was successfully isolated. The MrHc gene contained an open reading frame (ORF) of 1,992 nucleotides, encoding a protein of 663 amino acid residues with a molecular mass of 76.5 kDa. The deduced amino acid sequence contained distinct structural motifs of the haemocyanin superfamily, including an all‐alpha domain, a copper‐containing domain and an immunoglobulin‐like domain. Based on the phylogenetic analysis, the MrHC protein demonstrated a close relationship with the haemocyanins of Palaemon carinicauda and Macrobrachium nipponense. The MrHc gene was expressed in various shrimp tissues, including the hepatopancreas, gill, haemocytes, stomach and muscle. After Macrobrachium rosenbergii nodavirus (MrNV) challenge tests, the MrHc gene was up‐regulated 237‐fold at day 2. A recombinant protein of the MrHc immunoglobulin‐like domain exhibited antibacterial activity against Vibrio vulnificus, V. parahaemolyticus, Aeromonas caviae, A. veronii, A. hydrophila and Bacillus cereus. This study suggested that MrHc may play important roles in the shrimp innate immune response to MrNV infection and bacterial infection. 相似文献
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为了解团头鲂糖皮质激素受体(GR)在应激反应中的调控机制,本研究以皮质醇注射模拟应激事件,采集组织样品,常规检测血糖、血清皮质醇水平;利用荧光定量PCR检测了gr在不同组织中的表达丰度及其参与调控的功能基因的表达变化;并通过常规石蜡切片开展了组织病理学研究。结果显示,gr1在脾脏、鳃、头肾等组织中有较高的表达量,gr2则在肝、垂体、肠等组织中具有较高的表达丰度。应激恢复过程中,下丘脑gr1表达量存在波动,gr2在0 h表达显著上调,gr1/gr2值逐渐增大;垂体中gr1和gr2均呈现先升后降的趋势,gr1/gr2值在2 h达到峰值水平;头肾中gr1表达量有波动,而gr2在0 h和2 h的表达量显著低于其他检测点的表达量,gr1/gr2值逐渐减小;肝脏中gr1的表达量在0 h和2 h的表达量显著高于其他检测点,gr2表达有上下波动的现象,gr1/gr2值有减小的趋势,而pepck表达则出现了显著上调,在2 h达到峰值;皮肤中gr1先升高后降低,gr2在0 h和2 h的表达量显著低于其他检测点,gr1/gr2值在2～8 h维持在较大值,而occ表达呈现先增加后降低的变化趋势;鳃中gr1与gr2均在2 h时发生了显著上调,gr1/gr2值在2～8 h维持在较大值,occ表达峰值出现在2 h。组织学研究显示鳃丝有增生现象,肾间组织中类淋巴细胞增多,其他所检视组织无明显病理改变。应激反应过程中gr在不同组织不同亚型间存在不同的表达变化,以及和不同组织器官中相关功能基因的相关性显示了GR在相关调控中的复杂性,而组织学研究结果则表明了应激反应存在诱发病理变化的风险。 相似文献
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In order to determine the prevalence of pathogenic Escherichia coli, a total number of 155 E. coli isolates from aquaculture, clinical and veterinary sources were screened for seven pathogenic virulence markers and a house‐keeping gene by a polymerase chain reaction. The targeted virulence genes included eaeA of enteropathogenic E. coli, elt and est of enterotoxigenic E. coli (ETEC), ipaH of enteroinvasive E. coli, pCVD432 of enteroaggregative E. coli, stx, hlyA and eaeA of shigatoxigenic E. coli (STEC) and Enterohaemorrhagic E. coli. All the isolates were positive for phoA, the house‐keeping gene for E. coli. Among the 155 isolates, seven numbers (4.5%) harboured the virulence markers belonging to the pathogenic group ETEC and STEC. The virulent genes detected in these groups were elt, est, hlyA and stx. The sources of these virulence genes were fish (hlyA), shrimp (elt), feeder canal water (hlyA and elt) of aquaculture origin and from diarrhoea affected cow (hlyA, est and stx). The isolates with pathogenic traits belonged to the serogroups O6 or O29 and the remaining could not be typed. They showed resistance to two to four antibiotics out of the 12 antibiotics tested. Biotyping revealed that three isolates belonged to a single biotype (7333) and the remaining isolates were of diverse types. In conclusion, a molecular tool such as PCR proves as more effective tool for detection of this pathogen than the conventional methods. Detection of these emerging pathogens in aquaculture samples warrants for strict adherence to hygienic handling at retail outlets and proper cooking by the consumer before consumption. 相似文献
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Chang-Keun Kang Hye Young Park Mu-Chan Kim & Won Jae Lee 《Aquaculture Research》2006,37(12):1227-1237
A 4‐week feeding trial was conducted to test the use of marine yeasts purified from seawater and sediments as a dietary source in cultivating a cladoceran, Moina macrocopa, a potential alternative live food for fish larvae. Optimal growth conditions of two yeast strains were obtained for NaCl concentration, pH and temperature. Moina macrocopa was cultivated using different diets: marine yeasts (Debaryomyces hansenii Yeast‐14 and Candida austromarina Yeast‐16) and a commercial diet (Erythrobacter sp. Sπ‐I). The essential amino acids of both the yeast strains were nearly as great as those in M. microcopa. Further, the yeast‐fed M. macrocopa had essential amino acid profiles similar to the documented values for rotifers and Artemia enriched in microalgae and commercial diets. Erythrobacter sp. Sπ‐I lacked n‐3 polyunsaturated fatty acids, 20:5n‐3 and 22:6n‐3, which were also low but detected in both yeasts. An increase in the 20:5n‐3 [eicosapentaenoic acid (EPA)] and 22:6n‐3 [docosahexaenoic acid (DHA)] levels, compared with the levels in yeast strains themselves, was more pronounced in the 22:6n‐3 level of Moina fed the C. austromarina, resulting in a high DHA:EPA ratio. When the Moina diets were switched, their δ13C values shifted gradually towards the values of the switched diets. Diet switch from Erythrobacter sp. Sπ‐I to C. austromarina Yeast‐16 resulted in a more rapid turnover of Moina tissue carbon than that in the inverse case. When fed a mixed diet, the δ13C values of Moina tissue approached the value of marine yeasts immediately. These temporal changes in the δ13C values of Moina tissue indicate the preferential ingestion of marine yeasts and a selective assimilation of the carbon originated from marine yeasts. These findings suggest that marine yeasts, particularly C. austromarina Yeast‐16, are highly available to mass cultures of M. macrocopa, providing better nutritional and dietary values than the commercial diet (Erythrobacter sp. Sπ‐I). 相似文献
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Nor Zulkiply Amalina Zulperi Dzarifah Mohammad Noor Azmai Amal Mohd Termizi Yusof Mohd Zamri‐Saad Nurhidayu Al‐saari Mami Tanaka Sayaka Mino Tomoo Sawabe Md Yasin Ina‐Salwany 《Aquaculture Research》2019,50(11):3202-3210
Vibrio infections are common among marine fish and lead to serious problems in the aquaculture sector. This study reports a recent occurrence of Vibrio species (spp.) isolated from cultured groupers in Peninsular Malaysia using the gyrB and pyrH genes. A total of 147 Vibrio strains were successfully isolated from 77 (64%) groupers using culture method and subjected to gyrB and pyrH sequencing for species identification and confirmation. Results showed that 89% of Vibrio strains were identified and clustered to six groups of Vibrio spp., while 11% were not clustered to any Vibrio spp. using the gyrB sequences. Meanwhile, by analysis of the pyrH sequences all the 147 Vibrio strains (100%) were successfully identified and clustered into 11 groups of Vibrio spp., including the gyrB non‐identified strains. The pyrH gene provides a better resolution for identification of Vibrio spp. compared with the gyrB gene. Thus, the pyrH gene was more suitable for a rapid determination of Vibrio spp. distribution in Peninsular Malaysia. Using the pyrH gene, our study found higher prevalence of Vibrio vulnificus (33%), V. alginolyticus (24%) and V. parahaemolyticus (22%), followed by V. rotiferianus (5%), V. harveyi (3%), V. tubiashii (2%), V. campbellii (2%), V. ponticus (1%), V. diabolicus (1%), V. owensii (1%) and others Vibrio sp. (7%). Thus, the results of this study revealed that the occurrence of pathogenic vibrios among grouper fish is still high in Malaysian aquaculture. In addition, the pyrH gene was proved as a suitable marker for rapid identification of Vibrio species compared with the gyrB gene. 相似文献
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为探索脑型芳香化酶基因(cyp19a1b)在雌核发育三倍体鱼淇河鲫性别决定与分化过程中的作用,利用RACE方法克隆淇河鲫cyp19a1b基因cDNA全长序列,采用Real-time PCR分析其在不同组织、胚胎及胚后不同发育时期的表达情况,同时检测芳香化酶抑制剂Letrozole诱导性逆转及腹腔注射人绒毛膜促性腺激素(hCG)后cyp19a1b在脑中的表达情况。结果显示,淇河鲫cyp19a1b cDNA全长2 984 bp(GenBank ID:MF926270),包含132 bp5′非编码区,1 319 bp 3′非编码区,1 533 bp开放读码框,编码510个氨基酸残基;氨基酸序列比对及系统进化分析结果显示,淇河鲫Cyp19a1b与其他鲤科鱼类同源性较高,与哺乳类、爬行类等脊椎动物同源性较低,这与其分类地位一致;组织分布检测结果显示,cyp19a1b基因在淇河鲫脑中表达量最高,在卵巢等其他组织中表达量较低;Realtime PCR结果表明,在胚胎发育过程中cyp19a1b在外源精子刺激后从囊胚期开始上调,神经胚期达到最高,随后降低;出膜后随着发育的进行,该基因在脑中表达量逐步上调,在躯体中一直维持在较低水平;除此之外,伴随着Letrozole诱导的性逆转,cyp19a1b在脑中表达量降低;腹腔注射hCG可以促进cyp19a1b在脑中表达。研究表明,淇河鲫cyp19a1b基因可能通过参与神经系统的形成及神经内分泌活动,在性别决定与分化过程中起到一定的作用。 相似文献
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A.C. Puello-Cruz S. Mezo-Villalobos B. Gonzlez-Rodríguez D. Voltolina 《Aquaculture (Amsterdam, Netherlands)》2009,290(3-4):317-319
The copepod Pseudodiaptomus euryhalinus was fed 320 cells μL− 1 of monoalgal cultures of Chaetoceros muelleri, Nannochloropsis oculata, Isochrysis galbana, Tetraselmis suecica, or a commercial frozen concentrate of Tetraselmis sp., and the diet which gave the best production was compared in a second experiment to three mixed diets: C. muelleri:I. galbana supplied in 1:1 and 2:1 cell ratios and C. muelleri:I. galbana:frozen Tetraselmis sp. in 2:2:1 ratio. These gave better results than the cultures of N. oculata, I. galbana, T. suecica and the frozen Tetraselmis concentrate, but the production was similar to that obtained with C. muelleri supplied as a monoalgal diet, showing that the addition of C. muelleri may improve the performance of other monoalgal diets, whereas the addition of other microalgae is unlikely to improve the results obtained when C. muelleri is supplied as a monoalgal diet. 相似文献
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在脊椎动物中存在着2种twist基因,即twist1和twist2,二者对骨骼的发育具有调控作用。为探究唇?twist1和twist2与肌间刺骨化的相关性,实验克隆得到了唇?twist1和twist2基因cDNA序列。氨基酸序列比对结果显示,唇?TWIST1和TWIST2与其他鱼类保守性较高,都具有螺旋-环-螺旋(HLH)结构域和色氨酸-精氨酸(WR)结构域。系统进化树分析表明,唇?TWIST1和TWIST2与斑马鱼亲缘关系最为接近。通过荧光定量PCR检测发现唇?twist1和twist2基因在所有被检测的组织中均有表达,twist1在脑中的表达量最高,心脏中次之,在脾脏中表达量最低。twist2在皮肤和鳃中的表达量较高,在脾脏中的表达量最低。此外,twist1和twist2基因的表达量在肌间刺骨化的4个关键阶段发生显著变化。研究表明,twist基因与肌间刺骨化有一定的相关性。本研究首次在经济鱼类中克隆得到了twist基因,并揭示了twist基因与肌间刺骨化的相关性。研究结果将为进一步调查鱼类twist基因功能以及肌间刺形成的分子机制提供基础数据。 相似文献
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四川华鳊（Sinibrama taeniatus）是长江上游特有的小型经济鱼类,在野外其一年可以繁殖两次,但在26℃条件下进行人工养殖时其繁殖周期仅为14天,其中低温是限制其在野外繁殖的重要因素。为探究低温对四川华鳊卵巢发育的影响及调控方式,本研究对其产卵后雌鱼进行11℃的低温处理（LT组）,并与26℃条件下（OT组）卵巢发育状况进行对比。通过形态学和组织学观察发现LT组卵巢发育被抑制,发生退化,卵母细胞明显发育不良,出现典型闭锁卵泡的特征。对雌鱼血清中部分生殖激素及卵巢中相应受体基因的表达水平进行测定,发现低温使FSH和DHP水平显著下降,并严重抑制激素受体基因fshr,lhcgr,pgr,esr1,ar的表达。通过转录组测序和分析发现,在低温处理过程中类固醇激素合成,细胞生长增殖以及凋亡和自噬相关通路被显著富集,与低温的影响显著相关。低温下调了类固醇激素合成和卵母细胞发育相关基因StAR,cyp17a1,hsd3b,cyp19a2,hsd17b1,vtg1,zp4,mmp9,mmp15以及细胞生长增殖和抗凋亡相关基因ccni,cdk16,igf1r,egfr,nobox,bcl2的表达,上调了促凋亡基因bax,tnf,tp53的表达。上述结果表明,低温导致卵泡闭锁的原因一方面可能是低温抑制生殖激素的合成和受体基因的表达,使卵巢发育和卵母细胞生长增殖受限;另一方面,可能是低温促使卵泡发生自噬凋亡,最终导致卵泡发生退化闭锁。 相似文献