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1.
In this study, the effect of feeding different types and amounts of dietary fiber (DF) on luminal environment and morphology in the small and large intestine of sows was studied. Three diets, a low-fiber diet (LF) and 2 high-fiber diets (high fiber 1, HF1, and high fiber 2, HF2) were used. Diet LF (DF, 17%; soluble DF 4.6%) was based on wheat and barley, whereas the 2 high-fiber diets (HF1: DF, 43%; soluble DF, 11.0%; and HF2: DF, 45%; soluble DF, 7.6%) were based on wheat and barley supplemented with different coproducts from the vegetable food and agroindustry (HF1 and HF2: sugar beet pulp, potato pulp, and pectin residue; HF2: brewers spent grain, seed residue, and pea hull). The diets were fed for a 4-wk period to 12 sows (4 receiving each diet). Thereafter, the sows were killed 4 h postfeeding, and digesta and tissue samples were collected from various parts of the small and large intestine. The carbohydrates in the LF diet were well digested in the small intestine, resulting in less digesta in all segments of the intestinal tract. The fermentation of nonstarch polysaccharides in the large intestine was affected by the chemical composition and physicochemical properties. The digesta from pigs fed the LF diet provided low levels of fermentable carbohydrates that were depleted in proximal colon, whereas for pigs fed the 2 high-DF diets, the digesta was depleted of fermentable carbohydrates at more distal locations of the colon. The consequence was an increased retention time, greater DM percentage, decreased amount of material, and a decreased tissue weight after feeding the LF diet compared with the HF diets. The concentration of short-chain fatty acids was consistent with the fermentability of carbohydrates in the large intestine, but there was no effect of the dietary composition on the molar short-chain fatty acid proportions. It was further shown that feeding the diet providing the greatest amount of fermentable carbohydrates (diet HF1, which was high in soluble DF) resulted in significant morphological changes in the colon compared with the LF diet. 相似文献
2.
应用透射电镜技术，对15只正常中华鳖小肠和大肠黏膜上皮细胞的超微结构进行了研究。结果表明，中华鳖小肠黏膜上皮为单层柱状上皮，以高柱状吸收细胞为主，具有发达的微绒毛、线粒体、粗面内质网和膜包小囊泡，其结构的发达程度已接近高等哺乳动物和鸟类。吸收细胞之间可见少量低电子密度的亮细胞，其种类需进一步确定。杯状细胞明显可见，胞质内含大量黏液性颗粒，细胞游离面形成明显的微绒毛。内分泌细胞很少。大肠黏膜上皮细胞排列松散，全部由上下粗细不均的柱状细胞组成。大肠上皮内未见专门的杯状细胞，但上皮细胞顶部胞质内含大量黏液颗粒，细胞器分布于黏液颗粒团之下的胞质中。细胞间隙宽大明显，有利于黏膜免疫细胞出入上皮。小肠和大肠均未见腺体分布。 相似文献
3.
Observations were made on histological sections of the stomach and small intestine of seven rabbits infected with Trichostrongylus retortaeformis and from one uninfected control rabbit. At 12h post-infection, larvae were found in the small intestine. At first, only a few larvae were observed entering the mucosa through capillaries of the stroma of villi; the majority of larvae remained in the intestinal lumen, within mucus of the crypts. We consider that the presence of the worms in the stroma is the result of a larval migration. From a phyletic point of view, this migration is interpreted as an ancestral memory of the pulmonary migration seen in the primitive Strongylida. 相似文献
4.
小麦可溶性非淀粉多糖对大鼠肠道形态与功能的影响 总被引：2，自引：0，他引：2
试验采用单因素随机区组设计,研究在玉米淀粉-酪蛋白纯合日粮的基础上梯度添加0%、2%、3%、4%小麦可溶性非淀粉多糖(non-starch polysaccharides,NSP)对大鼠肠道形态与功能的影响。选用48只SD雄性大鼠随机分为4个处理,每处理6个重复,每重复2只大鼠。结果表明,随着日粮中可溶性NSP水平的提高,大鼠小肠各段绒毛高度线性降低(P0.05),大鼠十二指肠、空肠的隐窝深度线性增加(P0.05),大鼠小肠腐胺和精胺的含量线性提高(P0.05),大鼠盲肠乙酸、丙酸、丁酸及总挥发性脂肪酸的含量线性提高(P0.05)。 相似文献
5.
本试验旨在研究N-乙酰半胱氨酸(N-acetylcysteine,NAC)灌胃对超早期断奶仔猪血浆中游离氨基酸、小肠形态结构及功能的影响。将22头体重为(2.99±0.41)kg的7日龄断奶仔猪随机分为对照组和NAC组,每组11个重复,每个重复1头猪。在饲喂人工乳的基础上,NAC组每天每头按50 mg/kg体重进行NAC灌胃,对照组每天每头进行同体积的生理盐水灌胃。于试验第11天按0.1 g/kg体重的剂量给仔猪灌服D-木糖溶液,1 h后前腔静脉采血,然后屠宰取小肠组织样,刮取肠黏膜及制作组织切片。结果表明:与对照组相比,NAC组显著提高了超早期断奶仔猪平均日增重(P0.05);提高了超早期断奶仔猪血浆中谷氨酰胺、胱氨酸、γ-氨基丁酸水平(P0.05),降低了血浆中尿素氮、半胱氨酸、色氨酸水平和二胺氧化酶活力(P0.05);NAC灌胃提高了哺乳仔猪十二指肠黏膜的绒毛高度/隐窝深度(P0.05),有降低十二指肠隐窝深度的趋势(P0.10),有提高空肠绒毛宽度和回肠绒毛高度的趋势(P0.10)。在本试验条件下,NAC灌胃可改善小肠形态结构与屏障功能,提高氨基酸的利用率,调节血浆中部分氨基酸的含量。 相似文献
6.
Intestinal morphology and enzymatic activity in newly weaned pigs fed contrasting fiber concentrations and fiber properties 总被引：1，自引：0，他引：1
Hedemann MS Eskildsen M Laerke HN Pedersen C Lindberg JE Laurinen P Knudsen KE 《Journal of animal science》2006,84(6):1375-1386
The main objective of this study was to determine the effect of fiber source and concentration on morphological characteristics, mucin staining pattern, and mucosal enzyme activities in the gastrointestinal tract of pigs. The experiment included 50 pigs from 10 litters weaned at 4 wk of age (BW 8.6 +/- 1.4 kg) and divided into 5 treatment groups. Diets containing fiber of various physico-chemical properties and concentrations were formulated to contain 73, 104, or 145 g of dietary fiber/kg of DM. The diets were based on raw wheat and barley flours. Pectin and barley hulls, representing soluble and insoluble fiber sources, respectively, were used to increase the fiber concentration. The pigs were fed the experimental diets for 9 d, and then the pigs were euthanized and the entire gastrointestinal tract was removed. Tissue samples were taken from the mid and distal small intestine and from the mid colon. Inclusion of pectin in the diets significantly decreased (P < 0.001) ADFI and ADG compared with pigs fed no pectin. The villi and the crypts were shorter in pigs fed pectin-containing diets, but the villous height/crypt depth ratio was unaltered. Pectin significantly decreased the area of mucins in the crypts of the small intestine, indicating that the pigs fed the pectin-containing diet would probably be more susceptible to pathogenic bacteria, although this cannot be separated from the impact on ADFI. The lectin-binding pattern of the intestinal mucosa was unaffected by diet. The activity of lactase and maltase was increased in pigs fed diets with high fiber content, whereas sucrase activity was increased in pigs fed the pectin-containing diets. The activity of the peptidases, aminopeptidase N and dipeptidylpeptidase IV, was increased when feeding high fiber diets, whereas the activity of gamma-glutamyl transpeptidase remained unaffected by the experimental diets. In conclusion, the reduced feed intake observed with the pectin-containing diets could explain the lower villous height and crypt depth observed in this study. However, direct effects of pectin also are possible, and thus further study is warranted. Feeding pigs high insoluble fiber diets improved gut morphology by increasing villi length and increased mucosal enzyme activity when compared with pigs fed pectin-containing diets. The mucin content as determined by staining characteristics suggests that pigs fed high insoluble fiber diets might be better protected against pathogenic bacteria than pigs fed diets high in soluble fiber. 相似文献
7.
Kipar A Weber M Menger S Harmsen D 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2001,48(5):357-365
A 4-month-old male British Blue cat with catarrhal to haemorrhagic enteritis showed massive colonization of the stomach, small intestine and caecum with spiral-shaped bacilli. In the stomach, organisms were located in foveolae and gland lumina and within unaltered and degenerate epithelial cells. Inflammatory infiltration was moderate and T cell dominated. In the intestine, bacilli were found in the gut lumen, berween villi, in crypt lumina and within epithelial cells. Degeneration of crypt epithelial cells as well as crypt dilation and moderate to massive macrophage-dominated infiltration of the mucosa and submucosa were observed. Immunohistochemically, bacilli were positive with an antibody against Helicobacter. Ultrastructurally, the organisms strongly resembled 'Flexispira rappini', a spiral-shaped Helicobacter species known as a normal intestinal colonizer in dogs and mice. 相似文献
8.
Ghrelin在成年皖西白鹅小肠内的免疫组化定位研究 总被引：1，自引：0，他引：1
采用免疫组化SABC法并结合DAB显色技术研究了皖西白鹅小肠内Ghrelin阳性细胞及其分布。结果：①Ghrelin免疫阳性细胞主要位于小肠的黏膜层，而黏膜下层和肌层未见Ghrelin阳性细胞。这些阳性细胞，胞浆着色，主要有两类，一类为“闭合型”细胞，呈圆形或椭圆形，其顶部不露于腔面，另一类为“开放型”细胞，呈锥体型，细胞顶部达到腔面。②在十二指肠，“开放型”和“闭合型”两种阳性细胞均可观察到，而在空肠和回肠，主要为“闭合型”阳性细胞。产生Ghrelin的细胞在成年皖西白鹅小肠黏膜层有广泛的分布，揭示Ghrelin可能调节小肠的功能。 相似文献
9.
Nine calves, of which six had been challenged with an enteropathogenic strain of Escherichia coli, were found to be naturally infected with rotavirus. Rotavirus was recovered from the faeces of six calves and rotavirus antigen was detected in the intestinal mucosa of all calves. Stunting and fusion of villi were seen principally in the proximal and middle small intestine, where rotavirus antigen was detected by immunofluorescence. Typical lesions of enteric colibacillosis were found in the distal ileum of the challenged calves, associated with adhesion of the challenge strain of E coli to the mucosa. All samples were removed from the intestine under general anaesthesia and denudation of villi was not observed. However, following exsanguination and resampling at the same site in the small intestine of one calf, denudation was a constant feature. 相似文献
10.
G.T. Williamson M.R.C.V.S. G.W. Butler M.Sc. Fil.dr. 《New Zealand veterinary journal》2013,61(6):148-150
A method of cannulating the small intestine of the calf is described. Indwelling cannulas gave direct access to the intestinal lumen and mucosa, providing direct study of intestinal function and morphology. 相似文献
11.
Significance of a common extended mucosal interface observed in transverse small intestine sonograms
Delphine N. Rault DVM Juliette G. Besso DEDV Lilia Boulouha DVM Dominique Begon DVM Yannick Ruel DVM 《Veterinary radiology & ultrasound》2004,45(2):177-179
An echogenic stripe can be seen through the mucosal layer on either side of the ultrasonographic image of a bowel loop in cross-section. The mucosa is a continuous layer surrounding the intestinal lumen; thus it should appear as a continuous hypoechoic band with no bright stripe in it. The purpose of this study was to investigate the origin of this stripe and to determine whether it is an artifact. Intestinal cross-sectional images were obtained using different transducer types and frequencies. In vivo and in vitro, different angles of insonification were used and, in vitro, bowel loops were also fluid filled during an ultrasonographic examination, to assess the potential influence of intestinal shape and position on this stripe. Some of these loops were evaluated histologically to determine if the echogenic stripe had a histologic basis. The echogenic stripe was present only when the loop was flattened. It remained on each side of the maximal cross-sectional width and disappeared when this width was parallel to the ultrasound beam, or when the bowel loop had a completely round shape and was dilated. There was no influence of transducer type, shape, or frequency on the appearance of the line. Histologically, uneven and larger distance between the mucosal villi could be seen on either side of the bowel loop corresponding to the location of the echogenic stripe seen on the ultrasonographic images. In conclusion, the occasional echogenic stripe represents an interface within the mucosa due to altered position of villi on either side of the maximal intestinal cross-sectional width in collapsed bowel segment. 相似文献
12.
Kouichi Okamura Naoki Sasaki Takuya Kikuchi Aya Murata Inhyung Lee Haruo Yamada Hisashi Inokuma 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(2):157-160
The purpose of the present study was to evaluate the prokinetic effects of mosapride with non-invasive assessment of myoelectrical activity in the small intestine and caecum of healthy horses after jejunocaecostomy. Six horses underwent celiotomy and jejunocaecostomy, and were treated with mosapride (treated group) at 1.5 mg/kg per osos once daily for 5 days after surgery. The other six horses did not receive treatment and were used as controls (non-treated group). The electrointestinography (EIG) maximum amplitude was used to measure intestinal motility. Motility significantly decreased following surgery. In the treated group, the EIG maximum amplitude of the small intestine was significantly higher than in the controls from day 6~31 after treatment. These findings clearly indicate that mosapride could overcome the decline of intestinal motility after jejunocaecostomy in normal horses. 相似文献
13.
14.
The current study was conducted to investigate the effects of high dietary concentrations of Zn as zinc oxide and Cu as copper sulfate on the activity of digestive enzymes in the pancreas and the intestinal mucosa, intestinal morphology, and mucin histochemistry in pigs after weaning. Thirty-two pigs were weaned at 4 wk of age. The pigs were fed standard weaning diets supplemented with Zn (100 or 2,500 ppm) and Cu (0 or 175 ppm) in a 2 x 2 factorial arrangement of treatments for a 14-d period. In pancreatic tissue, the activity of amylase, carboxypeptidase A, chymotrypsin, trypsin, and lipase increased (P < 0.01) in pigs fed 2,500 ppm of Zn, whereas the activity of carboxypeptidase B and carboxylester hydrolase was unaffected. Copper had no effect on the activity of pancreatic enzymes. In small intestinal contents, the total activity of amylase and carboxypeptidase A was greater in pigs fed 100 ppm of Zn (P < 0.05), whereas feeding 2,500 ppm of Zn increased the chymotrypsin activity (P < 0.001). The remaining enzymes were unaffected by dietary Zn concentration. The villi were longer in the cranial small intestine (P < 0.001) in pigs fed 100 ppm of Zn than in pigs fed 2,500 ppm of Zn, but otherwise there were no clear effects of Zn and Cu supplementation on intestinal morphology. In the cranial small intestine, the activity of maltase (P < 0.001), sucrase (P < 0.001), and lactase was greater in pigs fed 100 ppm of Zn, even though there was a Zn x Cu interaction (P < 0.05) in lactase activity. In the middle and caudal small intestine, no clear differences between dietary treatments were observed. The activity of gamma-glutamyl transpeptidase in the intestinal mucosa was not affected by dietary Zn or Cu. In pigs fed 100 ppm of Zn, the activity of aminopeptidase N was greater in the caudal small intestine, but dietary Zn or Cu had no effect on aminopeptidase N in the cranial and middle small intestine. No effect of dietary Zn or Cu supplementation was found on carbohydrate histochemistry in the caudal small intestine, whereas high dietary Zn increased the area of neutral, acidic, and sulfomucins in the cecum (P < 0.01) and in the colon (P < 0.001). In summary, high dietary Zn increased the activity of several enzymes in the pancreatic tissue and increased the mucin staining area in the large intestine, whereas Cu had no clear effect on these variables. However, no definite answers were found as to how the growth promoting and diarrhea reducing effects of excess dietary Zn are exerted. 相似文献
15.
给8头生后3d的哺乳仔猪经口感染猪流行性腹泻病毒(PEDV)“吉”毒株,于感染后18、30、45和96h各扑杀2头,以透射电镜和扫描电镜观察了小肠粘膜上皮细胞及肠系膜淋巴结的超微结构。结果表明,小肠上皮细胞的病变因感染时间不同而有明显差异。上皮细胞的脱落和残留上皮细胞超微结构的破坏,以感染后30h最严重,病毒在这些上皮细胞内的增殖最显著。感染后45h,见有大量新生上皮细胞修补损伤的肠绒毛。感染后96h,小肠绒毛短缩、粗大乃至发生融合。实验仔猪肠系膜淋巴结内巨噬细胞和淋巴细胞的超微结构均遭到破坏,在巨噬细胞内见有PED冠状病毒粒子。 相似文献
16.
The small intestines of calves inoculated orally with the enteropathogenic strain of Escherichia coli 0101:K'B41',K99 were examined by electron microscopy at 3, 6, 12, 16, 21, 36, 69, 70 and 72 hours after inoculation. The challenge organism adhered to the mucosa of the distal small intestine from six hours post-inoculation. Bacteria were separated from the microvillous brush border by a gap of 200 to 300 nm in which bacterial fimbriae and the microvillous glycocalyx were seen. Bacteria never were found in epithelial cells but were present in macrophages in the lamina propria from 12 hours. At three and six hours, cytopathic changes were not seen in the small intestine, but from 12 hours epithelial cells on affected villi had blunt and thick microvilli and contained cytoplasmic inclusions. Epithelial cells were seen frequently in the process of extrusion from the villi, either singly, in small groups, or as ribbons of cells. Intervillous bridges, characteristic of villous fusion, were seen frequently from 69 hours. 相似文献
17.
A 4‐month‐old male British Blue cat with catarrhal to haemorrhagic enteritis showed massive colonization of the stomach, small intestine and caecum with spiral‐shaped bacilli. In the stomach, organisms were located in foveolae and gland lumina and within unaltered and degenerate epithelial cells. Inflammatory infiltration was moderate and T cell dominated. In the intestine, bacilli were found in the gut lumen, between villi, in crypt lumina and within epithelial cells. Degeneration of crypt epithelial cells as well as crypt dilation and moderate to massive macrophage‐dominated infiltration of the mucosa and submucosa were observed. Immunohistochemically, bacilli were positive with an antibody against Helicobacter. Ultrastructurally, the organisms strongly resembled ‘Flexispira rappini’, a spiral‐shaped Helicobacter species known as a normal intestinal colonizer in dogs and mice. 相似文献
18.
T Landsverk 《Acta veterinaria Scandinavica》1980,21(3):402-414
The histochemical distribution of selected enzymes were examined in the small intestine of 5 about 3-week-old normal calves fed on whole cow’s milk. Alkaline phosphatase and β-D-galactosidase (= lactase) in the epithelial brush border, and non-specific esterase in the cytoplasm showed a strong reaction in the villi of the anterior small intestine and a marked decrease in the posterior regions. Aminopeptidase in the brush border of the villi showed a reverse distribution, with the strongest reaction in the posterior small intestine. Adenosine-triphosphate-splitting enzyme in the epithelial brush border, acid phosphatase and succinate dehydrogenase in the cytoplasm of the epithelial cells gave a relatively uniform reaction in the villi throughout the small intestine. A fluoride-resistant acid phosphatase was demonstrated in the brush border of the villi in the anterior small intestine. The distribution of enzymes demonstrated in this study was generally compatible with the known absorptive functions of the various parts of the small intestine. 相似文献
19.
Several studies were carried out to investigate the influence of dietary cereals differing in soluble non starch polysaccharides (NSP) content and a xylanase preparation on selected bacterial parameters in the small intestine of broiler chicken. Compared to a maize diet colony forming units (CFU) of mucosa associated bacteria were higher in a wheat/rye diet, most notably for enterobacteria and enterococci. Xylanase supplementation to the wheat/rye diet generally led to lower CFU, especially in the first week of life. However, xylanase supplementation also displayed higher in vitro growth potentials for enterobacteria and enterococci. Bacterial growth of luminal samples in minimal media supplemented with selected NSP showed that the wheat/rye diet enhanced bacterial capacities to utilize NSP only in ileal samples. The xylanase application generally shifted respective maximum growth to the proximal part of the small intestine. The presence of soluble NSP from wheat or rye in the diet per se did not enhance bacterial NSP hydrolyzing enzyme activities in the small intestine, but xylanase supplementation resulted in higher 1,3-1,4-beta-glucanase activity. Compared to a maize diet the activity of bacterial bile salt hydrolases in samples of the small intestine was not increased due to inclusion of wheat/rye or triticale to the diet. However, xylanase supplementation led to a reduction with a corresponding increase of lipase activity. It was concluded that dietary cereals producing high intestinal viscosities lead to increased overall bacterial activity in the small intestine. The supplementation of a xylanase to cereal based diets producing high intestinal viscosity, changes composition and metabolic potential of bacterial populations and may specifically influence fat absorption in young animals. 相似文献