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1.
Ninety-six pure-bred Large White pigs weighing 80 to 100 kg were either not transported or transported for eight, 16 or 24 hours, and then either slaughtered immediately on arrival at the abattoir or kept in lairage for six hours before being slaughtered. The pigs travelling for eight, 16 or 24 hours lost 2.2, 2.0 and 4.3 per cent of liveweight, respectively, and the pigs travelling for 24 hours lost 2.6 per cent of hot carcase weight Both liveweight and hot carcase weight recovered during the lairage period although there was a net liveweight loss in comparison with the control animals. Only transport for 24 hours led to losses in carcase weight The concentration of non-esterified fatty acids increased with the time spent travelling. The concentrations of cortisol, creatine phosphokinase and lactate were all low in comparison with the levels found in commercially slaughtered animals. Plasma albumin and protein concentrations indicated that the animals were becoming dehydrated during the longer journeys. The animals lay down for most of the journey and appeared to be asleep. During the period in lairage, the animals transported for eight hours had two distinct periods of feeding and drinking but spent most of the time lying down, but those transported for 16 and 24 hours showed far more eating and drinking activity. All the groups appeared tired, but the urge to eat of the groups transported for 16 hours, and especially 24 hours appeared to be more important. There were behavioural and the physiological differences between the transported groups and the controls. Six hours in lairage with access to food and water allowed most of the physiological parameters to return to pretransport levels.  相似文献   

2.
Comparisons were made of the behaviour, physiology and meat quality of 24-week-old male veal calves (mean live weight 234 kg) that were slaughtered either at the veal unit (the control group) or following 3 h transportation at space allowances of either 0.7 or 0.95 m2 calf−1 and 1.5 h of lairage. Compared with nontransported calves, transported calves had a greater heart rate during the journey and during lairage; greater plasma cortisol concentration immediately after the journey, but not after 1.5 h of lairage; and greater plasma creatine kinase activity immediately after the journey and after 1.5 h of lairage. The transported calves did not lie down during the journey. There was no effect of space allowance on either the heart rate or frequency of potentially traumatic events during the journey or the plasma cortisol concentration and plasma creatine kinase activity sampled after the journey. There was no effect of transport on either carcase bruising or muscle pH 24 h after slaughter. Although compared with ‘on-farm’ slaughter, the transport and handling associated with the journey to the slaughterhouse were stressful to the calves; there were no adverse effects of transport on either carcase bruising or meat quality.  相似文献   

3.
A comparison was made of the effect of providing or denying water to steers during the last 20 h before slaughter on carcase weight, bruising, muscle pH, and during the dressing process on the numbers of rumens from which ingesta was split and the number of heads and tongues condemned because of contamination with ingesta. All cattle were offered water from arrival at the abattoir until the start of the treatment period. In one experiment the steers were rested for 24 or 48 h after a 120 km journey before being slaughtered, while in a second experiment they rested for 66 h after a 725 km journey. Giving steers access to water until they were slaughtered did not affect adversely carcase weight, bruise score, muscle pH or the incidence of ingesta spillage from rumens. The number of condemned heads and tongues was not consistently related to the availability of water. In the absence of any adverse effects it is recommended that cattle be provided with water during the lairage period.  相似文献   

4.
Keeping pigs in lairage provides a buffer to supply the slaughter line and allows the pigs to recover from the stress of transport. It has implications for the pigs' welfare by influencing their behaviour and ease of handling. In terms of pork quality, optimal lairage times for most pigs in the UK are probably one to three hours. Shorter lairage times are associated with more pale, soft, exudative (PSE) meat and longer times are associated with more dark, firm, dry (DFD) meat, more skin blemishes caused by fighting, and a lower carcase yield. However, when pigs have not been previously stressed by handling procedures, resting them in lairage may have no beneficial effects on the pigs' welfare or meat quality. At very high environmental temperatures there is evidence that a holding period of less than one hour may help to reduce the number of PSE carcases. At temperatures above 10 degrees C showering the pigs with water is beneficial. Fasting times need to be controlled to minimise the potential loss of carcase yield while reducing the volume of gut contents to reduce the risk of carcase contamination. Lairages can act as reservoirs of infection by pathogenic bacteria and there is evidence that longer holding times increase the risk of cross-contamination.  相似文献   

5.
A total of 150 Large White cross Landrace pigs (110-120 kg) of both sexes were used to investigate the effects of three different lairage times (0 h, 3 h, 9 h). Blood samples were collected at exanguination and cortisol, glucose, lactate, muscle enzymes and haematological parameters were determined. Post-mortem measurements of muscle pH were taken at 20 min, 2 h and 24 h from Longissimus thoracis and Semimembranosus. Lairage time showed a significant effect on pH24, internal muscle reflectance using the fibre optic probe (FOP24), red blood cells, neutrophils and lymphocytes, glucose and enzymatic activities. Changes in blood profile and meat quality parameters indicated that three hours of lairage in Spanish commercial conditions may reduce the amount of stress exhibited by pigs at slaughter and better meat quality can be obtained. No lairage or an excessively long lairage period without food may compromise animal welfare and meat quality.  相似文献   

6.
A 23 factorial design was used to examine the following treatments on the finishing of steers fed diets of 90% sorghum grain and 10% roughage: cottonseed hulls v. lucerne as the roughage component; nil v. 3% tallow; free-choice supplements of (a) sodium chloride, (b) 50/50 mixture of sodium chloride and rock phosphate, and (c) soil; a decrease in the crude protein level of the diet from 14 to 11% after body weight exceeded 350 kg.
Nine groups, each of 10 Shorthorn steers aged between 12 and 15 months, of an initial mean shrunk liveweight of 215.8 ± SE 0.35 kg were used. Individual steers were slaughtered either at 390 kg liveweight or after 177 days.
The mean growth rate was significantly higher in steers receiving cottonseed hulls than in those fed lucerne (1.27 v. 1.13 kg/day) and was significantly higher in those receiving tallow treatments than in those without tallow (1.28 v. 1.12 kg/day). Steers receiving 14% crude protein throughout showed an increased rate of carcase weight gain of 0.059 kg/day compared with steers receiving 11% from 350 kg body weight. The supplements had no effect on performance.
The overall mean carcase weight gain and dressing percentage were 0.68 kg/day and 59.0%, respectively.
Feed conversion (dry matter) was lower in steers receiving tallow than in those not receiving tallow (5.3 v. 6.3).
Steers fed lucerne exhibited intermittent mild to moderate bloat which did not require treatment.  相似文献   

7.
The objectives of the present experiment were 1) to study the effects of transport conditions and lairage duration on stress level, muscle glycolytic potential, and pork quality; and 2) to investigate whether the negative effects of high stress immediately preslaughter are affected by preceding handling factors (transport and lairage). In a 2 x 2 x 2 factorial design, halothane-free pigs (n = 384) were assigned to either short (50 min) and smooth or long (3 h) and rough transport; long (3 h) or short (<45 min) lairage; and minimal or high preslaughter stress. Pigs were processed in eight groups (48 pigs per group) on various days at a commercial plant. Blood samples were taken at exsanguination to measure plasma cortisol and lactate concentrations. Muscle pH and temperature were measured at 30 and 40 min, respectively, and both were measured at 3 h, postmortem. A LM sample was taken 135 min postmortem to estimate glycogen content and rate of glycolysis. Pork quality attributes were assessed 23 h postmortem. Short transport increased cortisol when followed by short lairage (transport x lairage; P < 0.01). Long transport, but not lairage (P > 0.30), tended to increase (P = 0.06) muscle glycolytic potential. Long transport tended to increase (P = 0.08) electrical conductivity, and decreased a* (P < 0.01) and b* (P < 0.02) values. Decreasing lairage from 3 h to <45 min decreased (P < 0.05) the L* value, but it did not (P > 0.10) affect other pork quality traits. High stress decreased (P < 0.001) muscle glycolytic potential, and increased (P < 0.001) plasma lactate, cortisol, muscle temperature, rate of pH decline, and ultimate pH. Except for decreased (P < 0.001) b* values, pork color was not (P > 0.40) affected by high stress, but water-holding properties (measured by electrical conductivity, filter paper moisture, and drip loss) were impaired (P < 0.001) by high stress. Fiber optic-measured light scattering and Warner-Bratzler shear force were not (P > 0.12) affected by any treatment. Comparisons with the "optimal" handling (short transport, long lairage, and minimal stress) revealed that, with regard to water-holding properties, the negative effects of high stress were aggravated by suboptimal transport and lairage conditions. High stress alone increased electrical conductivity by 56%, whereas high stress in combination with short lairage led to an 88% increase. However, high preslaughter stress contributed most and was the major factor responsible for reductions in pork quality.  相似文献   

8.
Changes in physiological and behavioral responses of goats to road transportation have been demonstrated, but effects of lairage and grazing on transported goats and the role of ascorbic acid (AA) on the effects have not been elucidated. The aims of the experiment were to investigate physiological, behavioral, lairage and grazing responses of goats transported by road and administered with AA. Experimental goats (n = 20) were administered with AA, whereas control goats (n = 20) were given sterile water before transportation by road for 12 hours. The goats were either grazed or held in lairage after the transportation. Excitability scores, grazing time, liveweight, and activities of serum enzymes were evaluated in goats that were grazed and those kept in lairage. The results obtained post-transportation showed that 12 hours of road transportation and lairage was stressful to the goats, and it decreased (P < 0.05) their excitability scores, grazing time, and liveweight. The activities of alanine aminotransferase, aspartate aminotransferase, and creatine phosphate kinase rose after the transportation, especially in the control goats, kept in lairage. Overall, the results showed that extensively raised goats, transported for 12 hours during the hot-dry season, require a lairage period of 7-11 days for their metabolism, behavior, and liveweights to return to baseline values. Goats that were allowed to graze after transportation required 3 days to return to baseline values, but those administered with AA before transportation and grazed after transportation required only 2 days to recover. The results demonstrated that keeping goats in the lairage after transportation was not beneficial. In conclusion, AA administration ameliorated stress because of transportation by road, and post-transportation grazing facilitated the recovery of the goats from the stress.  相似文献   

9.
Friesian steers were exposed to natural trichostrongyle infection and left untreated (controls) or treated with either a morantel sustained release bolus or fenbendazole at two week intervals (clean) in each of two consecutive grazing seasons. In the second grazing season the mean liveweight gains of the clean, morantel treated and control groups of cattle over 152 days were 105, 131 and 109 kg respectively (morantel treated greater than clean, control P less than 0.02). The cattle were slaughtered following an indoor fattening period. The dressed carcase weight, killing out percentage and related carcase measurements were superior in the treated groups. Eighty per cent of the clean and morantel treated cattle graded satisfactorily and qualified for subsidy payment whereas less than 30 per cent of the control cattle were successful. Rib joint analyses showed significantly lower total weight, eye muscle weight and area together with a higher bone content in control cattle.  相似文献   

10.
Commercial broiler chickens killed in two processing plants, one in the south of England, the other in Scotland, in two seasons (winter and summer) and on two occasions in each season, were used to investigate the effects of killing the birds immediately on arrival or holding them in lairage for one, two, three or four hours. The two most important consequences of holding the birds in lairage were that their body temperature increased and their liver glycogen was depleted. The body temperature increased with the time they were held in lairage, although most of the increase occurred in the first hour and the increase was greater in summer when ambient temperatures were higher. Liver glycogen depletion became apparent after about one to two hours in lairage. No evidence was obtained that the birds were significantly dehydrated or physically stressed by being kept longer in lairage.  相似文献   

11.
The effects of implantation with different anabolic agents on growth rate, behaviour, carcase parameters and testicular size of bulls were evaluated in an experiment with 361 Friesian bulls about three months old on three farms. Animals were allocated to one of the following treatments: (a) Control; (b) repeated implantation with 36 mg zeranol at intervals of 95 to 124 days; (c) repeated implantation with 20 mg oestradiol benzoate plus 140 mg trenbolone acetate as in (b); (d) repeated implantation with 20 mg oestradiol benzoate plus 200 mg progesterone as in (b); (e) implanted once with 45 mg oestradiol-17 beta. Daily liveweight gain, carcase weight, testicular size and behaviour of the animals were the parameters measured. Repeated implantation of young bulls with hormones, beginning at eight to 10 weeks old, increases liveweight gain and carcase weight from 0 to 15 per cent, reduces aggressive behaviour and testicular size and, in some instances, improves conformation and increases fat cover of the carcase.  相似文献   

12.
The objective of the present experiment was to investigate the effects of transportation, lairage, and preslaughter stressor treatment on glycolytic potential and pork quality of the glycolytic longissimus and the oxidative supraspinatus (SSP) or serratus ventralis (SV) muscles. In a 2 x 2 x 2 factorial design, 384 pigs were assigned randomly either to short (50 min) and smooth or long (3 h) and rough transport, long (3 h) or short (< 45 min) lairage, and minimal or high preslaughter stress. Muscle samples were taken from the LM at 135 min and from the SSP at 160 min postmortem for determination of the glycolytic potential and rate of glycolysis. At 23 h postmortem, pork quality was assessed in the LM and the SV. Effects of transport and lairage conditions were similar in both muscle types. Long transport increased (P < 0.01) the glycolytic potential and muscle lactate concentrations compared with short transport. Both long transportation and short lairage decreased (P < 0.01) redness (a* values) and yellowness (b* values) of the LM and SV. In combination with short lairage, long transport decreased (P < 0.05) pork lightness (lower L* values), and electrical conductivity was increased (P < 0.05) after long transport. Several interactions between stress level and muscle type (P < 0.001) were observed. High preslaughter stress decreased (P < 0.001) muscle glycogen in both the LM and SSP, but this decrease was greater in the LM. Lactate concentrations were increased (P < 0.001) only in the LM by high preslaughter stress. Increases in ultimate pH (P < 0.001) and decreases in a* values (P < 0.01) were greatest in the SV, whereas increases in electrical conductivity (P < 0.001) were greatest in the LM. The lack of interactions among transportation, lairage, and muscle type was attributed to the relatively minor differences in stress among treatments. It was concluded that, in glycolytic muscle types such as the LM, the high physical and psychological stress levels associated with stress in the immediate preslaughter period have a greater effect on the water-holding capacity of the meat and may promote PSE development. Conversely, oxidative muscle types tend to have higher ultimate pH values and produce DFD pork in response to intense physical activity and/or high psychological stress levels preslaughter.  相似文献   

13.
宰前休息方式对猪福利、血液成分及肉质的影响   总被引：6，自引：0，他引：6  
选取体质量约为100 kg、氟烷基因型为NN的长×大二元杂交猪60头,随机分为3组:不休息组,休息3 h组,休息3 h+玩具组,公母各半.根据评分标准分别记录休息过程中猪的精神状况.放血时收集血样,用于分析血浆中皮质醇、促肾上腺皮质激素(ACTH)浓度,乳酸脱氢酶、肌酸激酶活性,乳酸、血糖浓度;添加EDTA-K<,2>抗凝剂的血样用于血细胞分析.同时在放血时,测定血温.屠宰后.测定背最长肌24 h滴水损失;背最长肌(LM)、半膜肌(SM)45 min以及24 h的pH、色值、导电率.结果表明:①休息3 h+玩具组在3个时间段的精神评分要极显著高于休息3 h组,随着时间的延长猪都表现出趋于平静的趋势;②休息3 h+玩具组和休息组3 h组在皮质醇、促肾上腺皮质激素要显著低于不休组(P<0.05),相反乳酸脱氢酶、肌酸激酶要显著高于不休息组(P<0.05),而休息3h+玩具组与休息3 h组差别不显著(P<0.05);③3个休息处理组在肉质指标上没有出现显著差别(P<0.05);④休息3 h+玩具组、休息3 h组在红细胞数(RBC)、血红蛋白含量(HGB)均要显著低于不休息组(P<0.05);休息3h+玩具组在白细胞数(WBC)上显著高于不休组(P<0.05);休息3 h组的淋巴细胞绝对值(W-SCC)显著高于不休息组(P<0.05);而休息3 h+玩具组与休息3 h组在所有的免疫指标上都没有显著差异(P>0.05).根据上述的结果显示,宰前一定要让猪有一个休息的过程,这种措施能有效缓解猪的应激,并能恢复其免疫能力;在休息的过程中,添加部分福利玩具,至少对猪的精神状态有明显的好处.  相似文献   

14.
The times spent in transport and lairage by 124,036 lambs slaughtered in two plants in the south of England were recorded. About half of them travelled for up to 120 km, taking up to four hours, and three-quarters travelled not more than 300 km, the journeys taking up to about six hours. Bimodal frequency distributions of lairage time were apparent; overall one third of the lambs spent up to four hours in lairage but more than 40 per cent were kept for longer than 14 hours, while they were held overnight. Early season lambs tended to travel shorter distances to slaughter and spent less time in lairage than late season lambs. The average total times spent in transit and lairage were 22.3 hours and 13.3 hours at the two plants.  相似文献   

15.
Time in lairage needed by pigs to recover from the stress of transport.   总被引：6，自引：0，他引：6  
Two experiments were carried out in which a total of 602 pigs were slaughtered after being held in lairage for periods ranging from less than one hour to 21 hours. In the first experiment the pigs were handled under ideal conditions and slaughtered at the University of Bristol slaughterhouse; in the second the pigs were killed at a commercial plant. Blood samples collected at exsanguination were analysed for indices of stress. There were no consistent effects of time in lairage on the levels of lactate and creatine phosphokinase. Plasma cortisol and beta-endorphin levels were reduced by lairage for three hours or more in the first experiment and cortisol was reduced by lairage for two hours or more in the second; beta-endorphin was not measured in the second experiment. A period of rest in lairage allowed the pigs to recover from transport and the associated handling and the recovery appeared to be complete within two to three hours.  相似文献   

16.
Physiological and behavioural field studies on long distance road transport of 116 heifers, 135 bulls and 64 steers in 10 goose-necked double decked semitrailers from northern Germany to mediteranean ports showed different impacts of handling during pre-transport (i.e. collection of animals, weighing, loading), transport journey itself and post transport handling (i.e. lairage time) on coping strategies of the different categories of cattle. It was found loss of body weight in steers (-6.65%) coming from pasture was higher compared to bulls (-4.6%) during transport, but they recovered during lairage time in a better way. All categories of cattle showed catabolic energy metabolism during transport, but only in bulls and to a farer extent in heifers this leads to a tendency of a ketotic metabolism during second parts of transport and lairage time. During whole transport time no more than 20% of bulls and steers were laying down, and less than 5% were feeding during driving intervals. In all parts of transport general stress parameters like heart rate (with exception to steers) and cortisol were elevated as a part of adaptation to the transport environment, but indicating high physical and emotional loads on the animals with no resting possibilities. In this context animals have to be prepared carefully to be transported, i.e. in reference to energy and fluid balance, and to be feed in sufficient time intervals (breaks) and lengths to maintain fundamental behavioural and physiological needs of the animals during transport. The lairage facilities are very important for the bulls and in the case of heifers the feeding regime during lairage time must be improved to ensure the possibility for a real resting and recovery of the animals after transport.  相似文献   

17.
Long distance transportation of weaned piglets (Sus scrofa) is increasingly common in the united states and may result in delayed eating, drinking, or normal social behaviors. A potential solution is a mid-journey rest (lairage). The objective of this study was to determine if a lairage altered behavior after a 16-h transport. Pigs that weighed approximately 18 kg each (n = 894) were housed in 16 pens with 8 pens per treatment. Lairaged pigs were transported for 8 h and given an 8-h rest with food and water, whereas control pigs were transported continuously for 16 h. The heaviest, the lightest, and 2 average-BW pigs relative to the average weight of the pen were observed by video recording for 24 h immediately before and after transport, and during d 6 and 13 after transport. Postures (lying, sitting, and standing) were recorded using 10-min-interval scan sampling, and behavioral categories included inactivity, activities (eating, drinking, alert, manipulating pen, rooting, and walking) and social interactions (aggression, belly nosing, playing, tail biting, and positive social behaviors). In both treatments, sitting occurred most before transport (P < 0.01) than at other times, but did not differ between treatments. Standing increased (time effect; P < 0.01) for both treatments immediately after transport through d 6, but returned to pre-transport values by d 13. In contrast, lying decreased (time effect; P < 0.01) after transport, but returned to above pre-transport values by d 13. Time effects were evident for activity (P < 0.01), pen manipulation (P = 0.05), rooting (P < 0.01), initiation of belly-nosing (P = 0.01), and receiving belly-nosing (P = 0.03); however, initiation of aggression did not differ for day (P = 0.19) or treatment (P = 0.56). Lairaged pigs initiated more (P = 0.05) play than continuously transported pigs, but no differences (P = 0.84) were seen in receipt of play behavior. Pigs that were to be transported for 16 h continuously walked less pre-transport, walked more post-transport (treatment × time interaction; P = 0.02), and drank less pre-transport, but drank more on all days post-transport compared with the lairage group (treatment × time interaction; P = 0.001). This study indicated that extended transport without lairage alters some swine behaviors relevant to production (water consumption) and demonstrated that a long-duration transport, regardless of the mid-journey lairage treatment, affects a number of behaviors up to 13 d after transportation.  相似文献   

18.
In this study the effects of barren vs enriched housing conditions of pigs on their behavior during the lairage period (2-h holding period before slaughter), carcass characteristics, postmortem muscle metabolism, and meat quality were studied. The barren housing system was defined by common intensive housing conditions (i.e., with slatted floors and recommended space allowances), whereas the enriched environment incorporated extra space and straw for manipulation. Salivary cortisol concentrations were measured before transport and at the end of the lairage period. During the lairage period the percentage of time spent walking and fighting by the pigs was registered. Carcass characteristics such as weight, meat percentage, and backfat thickness were determined. At 5 min, 45 min, 4 h, and 24 h postmortem, pH, temperature, and lactate concentrations were determined in the longissimus lumborum (LL) and biceps femoris (BF) muscles. Capillarization of the muscle, mean muscle fiber area, and color and drip loss after 2 and 5 d of storage were determined for both muscle types. Pigs from the barren environment had a significantly higher increase in cortisol from farm to slaughter, but no differences in behavior were observed during the lairage period. Carcass characteristics did not differ between pigs from barren and those from enriched housing conditions. Postmortem lactate formation was significantly lower in LL muscles of enriched pigs at 4 and 24 h postmortem. Capillary density and mean muscle fiber area did not differ between the groups of pigs. The percentage of drip loss at 2 and 5 d after storage of LL muscle samples from enriched-housed pigs was significantly lower than that of the barren-housed pigs. Similar tendencies were found for the BF muscle from pigs kept in an enriched environment, but these were not statistically significant. The housing system did not affect meat color. It is concluded that on-farm improvement of animal welfare by environmental enrichment can also lead to beneficial economic effects after slaughter by improving the water-holding capacity of pork.  相似文献   

19.
The objective of the study was to investigate the physiological, haematological and immunological responses of weanling heifers transported from Ireland to a feedlot in Spain, and of weanling bulls transported from Ireland to a feedlot in Italy. Physiological variables (including interferon-γ production, cortisol, protein, urea, white blood cell numbers and differentials, and acute phase proteins (haptoglobin and fibrinogen) were used to evaluate the welfare status of animals, before, during and after the respective transport journeys. Age-matched control animals were blood sampled for the same measurements at times corresponding to the transported animals that were retained in Ireland. Heifers transported to Spain lost 7.6% of their initial live weight during the sea crossing to France. However, by the time of their arrival in Spain they had regained 3.3% of their initial live weight and had fully recovered to their pre-transport live weight values within 6 days of arriving in Spain. Weanling bulls lost 7.0% of their live weight during the sea crossing from Ireland to France. The live weight loss in control animals ranged from 1% to 2% during the same period. The percentage of time that bulls spent lying was 63.5% for the sea journey and 35.4% for the journey from the French lairage to the Italian feedlot. The average daily gain (kg) of transported animals was greater (P ≤ 0.05) than control animals from day 11 to 38 (Spain) and day 11 to 40 (Italy), respectively. While transient changes in physiological, haematological and immunological variables were found in the transported and control animals relative to baseline levels, the values were within the normal physiological range for the age and weight of animals involved. Physiological measurements made after the road and sea journeys indicated that the 24h rest in the lairage, with hay and water freely available, allowed animals to recover substantially.  相似文献   

20.
During the transport of salmon (Salmo salar), in a well-boat, 10 fish were sampled at each of six stages: in cages after crowding at the farm (stage 1), in the well-boat after loading (stage 2), in the well-boat after eight hours transport and before unloading (stage 3), in the resting cages immediately after finishing unloading (stage 4), after 24 hours resting in cages, (stage 5) and in the processing plant after pumping from the resting cages (stage 6). The water in the well-boat was at ambient temperature with recirculation to the sea. At each stage the fish were stunned percussively and bled by gill cutting. Immediately after death, and then every three hours for 18 hours, the muscle pH and rigor index of the fish were measured. At successive stages the initial muscle pH of the fish decreased, except for a slight gain in stage 5, after they had been rested for 24 hours. The lowest initial muscle pH was observed at stage 6. The fishes' rigor index showed that rigor developed more quickly at each successive stage, except for a slight decrease in rate at stage 5, attributable to the recovery of muscle reserves.  相似文献   

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