共查询到20条相似文献，搜索用时 171 毫秒
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高效液相色谱法测定博落回注射液中血根碱和白屈菜红碱的含量 总被引：1，自引：0，他引：1
利用高效液相色谱法测定博落回注射液中血根碱和白屈菜红碱的含量。采用Agilent1100型高效液相色谱仪,色谱柱为AgilentEcpliseXDB—C18（5μm,4．6mm×250mm）,流动相为三乙胺溶液-乙腈（75：25,V／V）,流速1．0mL／min;检测波长270nm;柱温35℃。结果表明：血根碱在0．5～100μg／mL范围内线性关系良好（R2=0．9999）,平均加样回收率（n=3）为96．1％～97．9％,RSD为0．8％～1．8％;白屈菜红碱在0．5～100μg／mL范围内线性关系良好（R2=1）,平均加样回收率（n=3）为96．8％-98．8％,RSD为0．6％～1．0％。本法简单快捷,精密度好,可为制定质控标准提供参考。 相似文献
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建立了高效液相色谱法测定地昔尼尔的含量和有关物质的检测方法。采用c,。色谱柱（5“m,4．6mm×250mm）,流动相为甲醇一水（30：70）,流速1．0mL／min,检测波长269nm,进样量20斗L,柱温25℃。该方法的线性范围为1．01～20．16txg／mL（r=0．9998）;最低检出限2ng;精密度的RSD为0．56％（n=6）;平均加样回收率为99．66％（n=9）。该方法简便灵敏,结果准确可靠,可用于地昔尼尔原料的含量及有关物质的测定。 相似文献
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建立了板蓝根注射液中（R,S）-告依春含量测定的高效液相色谱方法。采用Waters XBridgeC18色谱柱（150mm×4．6mm,5μm）,甲醇-0．02％磷酸溶液（7：93）为流动相,流速为1．0mL／min,检测波长245nm,柱温30℃。在1-40μg／mL浓度范围内线性关系良好（r=0．9998）,系统精密度RSD为0．31％（n=6）,平均回收率为98．6％（RSD=0．36％）。本方法重现性好,可用于该制剂的质量控制。 相似文献
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饲料中盐酸克伦特罗的高效液相色谱法测定 总被引：9，自引：0，他引：9
用高效液相色谱法测定饲料中盐酸克伦特罗的含量。选用ShimpackCLC－ODS分析柱（46mm×150mm）,流动相：甲醇：水＝75：25,检测波长：243nm,流速：1ml／min。盐酸克伦特罗浓度在0．1－2．0μg／ml之间具有良好线性关系,相关系数0．9996。平均回收率为（101．2±9．75）％。该法快速、准确、重现性好。 相似文献
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建立了癸氧喹酯原料药、固体分散体含量测定的高效液相色谱一紫外检测法。采用DiamonsilC18（4．6mm×150mm,5μm）反相色谱柱,以甲醇一水（95：5）溶液（用稀盐酸调pH值至3．5）为流动相,检测波长330nm,流速0．6mL／min,外标法定量。结果显示该方法最低检测限为0．0025p,g／mL,在0．5～10斗μmL浓度范围内,线性关系良好（A=11405C＋15821,R=0．999）,加样平均回收率99．61％,RSD为0．90％。此方法可用于癸氧喹酯的含量测定,适用于该药品固体分散体生产的质量控制。 相似文献
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建立了HPLC—ECD法测定大观霉素含量的分析方法。采用高效液相色谱-电化学检测器,Waters XBridge C18色谱柱（4．6mm×250mm,5μm）,以pH3．2的革酸盐缓冲溶液-乙腈（1000：105,V：V）为流动相,流速1．0mL／min;柱后衍生溶液为21g／L的NaOH溶液,流速0．5mL／min;ECD系统参数：极性为阳性,检波＋0．12V,氧化电位＋0．70V,还原电位-0．60V,范围5μA。结果表明,大观霉素及4R一二氢大观霉素在40～320μg／mL范围内呈良好的线性关系（大观霉素r=0．9999,4R-二氢大观霉素r=0．9992）,平均回收率为100．0％（n=9）,RSD为0．2％。该方法解决了微生物效价测定大观霉素含量偏高的难题,且操作简便、结果准确,可用于大观霉素及相关制剂产品的质量控制。 相似文献
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通过对提取液、净化方式的优化,色谱柱的选择建立饲料中三聚氰胺的高效液相色谱质谱联用法．针对相应的提取液用正交试验筛选最佳的提取方案。样品经乙腈水溶液（70：30．V/V）超声提取。离心后取上清液与等体积乙腈混匀．冷冻离心后直接过滤膜即可上机测定。经Waters Atlantis HIUC Silica（150mm×2．1mm,5um）分离,选择反应监测（SRM）正离子模式测定。结果表明,该方法在三聚氰胺含量为5．0～50．0ng／mL范围内线性关系良好．线性方程为Y=8958．7X-3327．8．R2=0．9996以三倍信噪比计算方法最低检出限为0．01mg／kg．平均加标回收率为：91．7％～98．5％,RSD〈5．0％。该法前处理快速简便,净化效果好。准确度和精密度高,可用于饲料中三聚氰胺的测定。 相似文献
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高效液相色谱法测定癸氧喹酯溶液的含量 总被引：1，自引：0，他引：1
建立了癸氧喹酯溶液含量测定的高效液相色谱方法。采用Zorbax Eclipse C18色谱柱（4．6mm×150mm,5μm）,以甲醇-乙腈-冰乙酸（45：55：0．1）溶液（用三乙胺调pH值至7．0,每1000mL再加入1．0g硝酸钙）为流动相,检测波长267nm,流速为1．0mL/min,外标法定量。该方法最低检测限为0．05g／mL,在1—100μg／mL浓度范围内线性关系良好（r^2=0．9999）,平均回收率99．67％,可用于癸氧喹酯溶液的含量测定。 相似文献
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采用高效液相色谱法（HPLC）测定复方聚维酮碘凝胶中氢化可的松的含量。以C18（4.6mm×250mm,5μm）为色谱柱,乙腈-甲醇-醋酸铵（20：45：35）为流动相,流速1mL／min,检测波长240nm,柱温37℃。结果显示：氢化可的松在0．025～0．122mg／mL范围内呈良好的线性关系（r=0．9993）,回收率为96．06％,相对标准偏差（RSD）为0．45％。试验表明,采用高效液相色谱法测定复方聚维酮碘凝胶中氢化可的松的含量灵敏、快速、准确,可用于该复方制剂的质量控制或检验分析。 相似文献
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True digestibility of lysine (LYS) in crystalline L-LYS.HCl and casein determined in cecectomized adult roosters was not significantly different from 100%. Subsequent 9-d growth assays were conducted to determine the bioavailability of LYS in casein, in L-LYS.HCl, and in a mixture of crystalline amino acids simulating casein's amino acid profile. Based on slope-ratio methodology, LYS bioavailability relative to casein was estimated to be 101.4 and 99.9% in L-LYS.HCl and in the casein-simulated amino acid mixture, respectively. Procedures also were developed for assessment of LYS bioavailability in crystalline L-LYS.HCl by comparing growth responses to intraperitoneally injected (IP-LYS) and crop-intubated LYS (CI-LYS) in chicks fed LYS-deficient corn-sesame meal diets. Graded increments of pH-adjusted L-LYS.HCl were administered twice daily in .5-ml doses during the course of 8-d growth assays. Chicks receiving CI-LYS also received .5 ml of IP saline at each dosing, and those receiving IP-LYS also received CI saline at each dosing. Slope-ratio multiple linear regression of gain (g) regressed on LYS administered (mg) was assessed for both ad libitum-fed and meal-fed chicks. Linear growth responses to LYS were obtained with both routes of administration and in both feeding regimens. The CI-LYS slopes ranged from 103 to 125% of those obtained with IP-LYS administration. These results support the view that crystalline L-LYS.HCl is 100% digestible and bioavailable. 相似文献
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1. A broiler experiment was conducted to assess the effectiveness of L-lysine.H2SO4 relative to L-lysine.HCl. Four concentrations of L-lysine.H2SO4 and L-lysine.HCl (0.9, 1.8, 2.7 and 3.6 g/kg diet) were each added to a basal diet that met the nutrient requirements of broilers except for lysine. 2. Birds responded significantly to the supplements in daily gain, feed intake, feed conversion efficiency, nitrogen retention and plasma urea nitrogen during each period (d 4 to 21, d 22 to 42 and d 4 to 42). 3. Regression analysis showed that the bioefficacy of L-lysine.H2SO4 relative to L-lysine.HCl was 0.93, 0.86 and 0.95 for daily gain, feed conversion efficiency and nitrogen retention, respectively, during the starter period (d 4 to 21), and was 1.01, 1.36 and 1.06, respectively, during the grower period (d 22 to 42). It was 0.99, 1.07 and 1.03, respectively, for the overall period (d 4 to 42), when the bioefficacy of L-lysine.HCl was set at 1.0. 4. The bioefficacy of L-lysine.H2SO4 differed with different response criteria. The average bioavailability of L-lysine.H2SO4 relative to L-lysine.HCl was 1.03 on an equimolar basis in the present study. In conclusion, L-lysine.H2SO4 and L-lysine.HCl are equally efficacious to broiler chickens. 相似文献
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通过50 L发酵罐试验,研究了培养条件(接种量、初始氨氮、初糖浓度)对L-赖氨酸发酵的影响。研究结果表明:发酵周期40 h,最佳培养条件:10%接种量、0.25%初始氨氮、3.5%初糖浓度,发酵最终平均产L-赖氨酸175 g/L。 相似文献
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Smiricky-Tjardes MR Mavromichalis I Albin DM Wubben JE Rademacher M Gabert VM 《Journal of animal science》2004,82(9):2610-2614
A pig growth assay was conducted to determine the relative biological value (RBV) of lysine from L-lysine sulfate compared with feed-grade L-lysine HCl. One hundred nursery pigs with an average initial BW of 9.5 +/- 1.5 kg were blocked by BW and gender and allotted randomly to five dietary treatments in five replicates of four pigs per pen. A corn-peanut meal diet containing 0.6% total lysine (as-fed basis) was supplemented with two levels (0.1 and 0.2%) of lysine from L-lysine-HCl or L-lysine sulfate. The RBV of L-lysine sulfate was determined using multiple regression slope-ratio methodology, with ADG and G:F as the response criteria. At the tested levels, linear responses for gain and G:F were obtained from increments of lysine from the two lysine sources. When ADG was regressed on supplemental lysine intake, the RBV of lysine in L-lysine sulfate was 99% of the RBV of lysine in L-lysine HCl. When G:F was regressed on supplemental lysine intake, the RBV of lysine in L-lysine sulfate was 97% of the RBV of lysine in L-lysine-HCl. The t-test analysis revealed that the RBV of lysine in L-lysine sulfate was not significantly different from the RBV of lysine in L-lysine HCl, which was assumed to be 100% bioavailable. In conclusion, L-lysine sulfate can replace L-lysine HCl in diets for growing swine. 相似文献
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OBJECTIVE: To examine the effects of orally administered L-lysine on clinical signs of feline herpesvirus type 1 (FHV-1) infection and ocular shedding of FHV-1 in latently infected cats. ANIMALS: 14 young adult, FHV-1-naive cats. PROCEDURE: Five months after primary conjunctival inoculation with FHV-1, cats were rehoused and assigned to receive 400 mg of L-lysine in food once daily for 30 days or food only. On day 15, all cats received methylprednisolone to induce viral reactivation. Clinical signs of infection were graded, and viral shedding was assessed by a polymerase chain reaction assay throughout our study. Peak and trough plasma amino acid concentrations were assessed on day 30. RESULTS: Fewer cats and eyes were affected by conjunctivitis, and onset of clinical signs of infection was delayed on average by 7 days in cats receiving L-lysine, compared with cats in the control group; however, significant differences between groups were not demonstrated. Significantly fewer viral shedding episodes were identified in the treatment group cats, compared with the control group cats, after rehousing but not following corticosteroid-induced viral reactivation. Mean plasma L-lysine concentration was significantly increased at 3 hours but not at 24 hours after L-lysine administration. Plasma arginine concentration was not significantly altered. CONCLUSIONS AND CLINICAL RELEVANCE: Once daily oral administration of 400 mg of L-lysine to cats latently infected with FHV-1 was associated with reduced viral shedding following changes in housing and husbandry but not following corticosteroid administration. This dose caused a significant but short-term increase in plasma L-lysine concentration without altering plasma arginine concentration or inducing adverse clinical effects. 相似文献
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Maggs DJ Collins BK Thorne JG Nasisse MP 《American journal of veterinary research》2000,61(12):1474-1478
OBJECTIVE: To determine the effects of various concentrations of L-lysine and L-arginine on in vitro replication of feline herpesvirus type-1 (FHV-1). SAMPLE POPULATION: Cultured Crandell-Reese feline kidney (CRFK) cells and FHV-1 strain 727. PROCEDURE: Uninfected CRFK cells or CRFK cells infected with FHV-1 were cultured in Dulbecco's modified Eagle's medium or in 1 of 7 test media containing various concentrations of lysine and arginine. Viral titer and CRFK growth rate were assessed in each medium. RESULTS: Media depleted of arginine almost completely inhibited viral replication, whereas 2.5 or 5.0 microg of arginine/ml of media was associated with a significant increase in FHV-1 replication. In media with 2.5 microg of arginine/ml, supplementation with 200 or 300 microg of lysine/ml reduced viral replication by 34.2 and 53.9%, respectively. This effect was not seen in media containing 5.0 microg of arginine/ml. Growth rates of CRFK cells also were suppressed in media containing these concentrations of amino acids, but they were not significantly different from each other. CONCLUSIONS AND CLINICAL RELEVANCE: Arginine exerts a substantial growth-promoting effect on FHV-1. Supplementation of viral culture medium with lysine attenuates this growth-promoting effect in media containing low concentrations of arginine. Analysis of data from this study indicates that high concentrations of lysine reduce in vitro replication of FHV-1 but only in media containing low concentrations of arginine. Clinical trials will be necessary to determine whether supplemental administration of lysine, with or without arginine restriction, will be useful in the management of cats with FHV-1 infections. 相似文献
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MAO Ran LI Qing-quan SHI Yan LAN Xiang-li WANG Min ZONG Ze-jun XIA Guo-zhong HAN Tian-long 《中国畜牧兽医》2015,42(12):3308-3314
In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3 health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels (0,0.1,0.2,0.3,0.4 and 0.5 g) L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78 h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1 and 0.2 g L-lysine were higher than other groups,and that in 0.4 and 0.5 g L-lysine groups decreased faster than other groups;The sperm motility in 0.5 g L-lysine group reduced to 0 at 30 h,and it reduced to 0 at 48 h in the group added 0.4 g L-lysine;The effective survival time and survival index in the group added 0.1 g L-lysine was higher than other groups (P< 0.05);The membrane integrity of 0.1 g group was the highest,that had no significant difference with control group from 0 to 54 h (P >0.05),while after 72 h the two groups had significant differences (P< 0.05).The membrane integrity in 0.4 and 0.5 g groups were the worst,and that was significant different with other groups (P< 0.05);The acrosome integrity of 0.1 g group was the highest which had no significant difference with control group (P >0.05),0.4 and 0.5 g groups were the worst,the difference between them was not significant (P >0.05),while was extremely significant difference with other groups except for 0 and 6 h (P< 0.01).The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2 g,sperm quality was significantly decreased,adding 0.1 g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state. 相似文献