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1.
The expected temperature rise in late summer/early autumn can change the conditions for acclimation and affect the winter survival of perennial crops. This study examined the effect of the temperature just before the onset of cold acclimation (pre‐acclimation) on freezing tolerance of timothy (Phleum pratense L.), perennial ryegrass (Lolium perenne L.) and red clover (Trifolium pratense L.) populations (both cultivars and breeding populations) adapted to either northern or southern parts of Norway. Using phytotron experiments, we studied whether increasing pre‐acclimation temperature delays growth cessation, affects photoacclimation and reduces freezing tolerance. Furthermore, we assessed whether these effects were related to the latitudinal adaptation of the plant material. The results showed that a rise in pre‐acclimation temperature decreased both cold acclimation capacity and photoacclimation in these species. This affected the freezing tolerance, which was reduced significantly more in northern‐adapted population of timothy and perennial ryegrass compared with southern‐adapted populations. Red clover was less affected by temperature changes than the grasses.  相似文献   

2.
Performance of Timothy-based Grass/Legume Mixtures in Cold Winter Region   总被引：1，自引：0，他引：1  
This study sought to identify grass/legume mixtures that increase the yield and persistence of forage stands with improved nutritive quality in cold‐winter regions, compared with the standard mixture of timothy (Phleum pratense L.)/red clover (Trifolium pratense L.)/alsike clover (Trifolium hybridum L.). Timothy was mixed with either perennial ryegrass (Lolium perenne L.), meadow fescue (Festuca pratensis L.) or Kentucky bluegrass (Poa pratensis L.). The legumes in mixtures were red clover, alfalfa (Medicago sativa L.) or white clover (Trifolium repens L.). Averaged over three production years, the majority of mixtures had greater dry matter (DM) yields than the standard (8.35 t ha?1). Timothy, grown alone and in three mixtures, outyielded the standard by 19–30 %. Yield reductions in mixtures over the 3‐year period were greatest with red clover, and least with bluegrass. Mixtures with alfalfa were highest in nitrogen (28.4 g kg?1), while grasses grown alone (24.6 g kg?1) and the standard mixture (25.1 g kg?1) were the lowest in N. Mixtures with red clover or alfalfa had the least neutral detergent fibre (NDF), averaging 418 and 429 g kg?1 respectively. Mixtures including white clover were initially low in NDF at 347 g kg?1 in year 1 but increased to 550 g kg?1 in year 3 as white clover composition declined in the sward.  相似文献   

3.
Heathcliffe Riday 《Euphytica》2009,170(3):339-345
Red clover (Trifolium pratense L.) forage yield remains a prime breeding target for improved variety development. In a world of decreasing forage legume breeding resources, rapidly and cheaply phenotyping plants for the highly quantitative trait of forage yield is vital. Many red clover selection programs are based on space planted nurseries. The objectives of this study were to determine: (1) the accuracy of visual forage yield scores in predicting actual forage yield; (2) the nature of the relationship between visual scores and actual measurements; and (3) The repeatability of visual scores between different evaluators. Twenty-seven halfsib families were transplanted at two locations in three replicates of six plant plots. Individual plant fresh weights and visual scores, by two evaluators, for forage yield were taken. On an individual plant basis visual forage yield scores showed an exponential relationship with actual fresh weights. Individual plant visual scores were very accurate with a pseudo-R 2 of 0.79 observed for the exponential model. On an entry mean basis using a linear model, visual scores could explain 90% of the variation of actual fresh weights. Agreement among evaluators scoring the same plants was very high with coefficients of determination at 0.84 for individual plants and as high as 0.96 on an entry mean basis. This study suggests that visual scores of plants in space planted red clover breeding nurseries are basically as accurate as measuring actual yields and that plants can be consistently scored the same by different evaluators.  相似文献   

4.
UK livestock agriculture can significantly reduce its protein imports by increasing the amount of forage based protein grown on-farm. Forage legumes such as red clover (Trifolium pratense L.) produce high dry matter yields of quality forage but currently available varieties lack persistence, particularly under grazing. To assess the impact of red clover persistence on protein yield, diploid red clover populations selected for improved persistence were compared with a range of commercially available varieties. All populations were grown over four harvest years in mixed swards with either perennial ryegrass (Lolium perenne L.) or perennial plus hybrid ryegrass (L. boucheanum Kunth). Red clover and total sward dry matter (DM) herbage yields were measured in Years 1–4, red clover plant survival in Years 3 and 4 and herbage protein (CP) yield and concentration in Years 2 and 4. In general, red clover DM yield in year 4 (3.4 t ha−1) was lower than in year 1 (13.9 t ha−1) but the red clover populations differed in the extent of this decline. Differences in the persistence of the red clover populations in terms of plant survival and yield were reflected in the contribution of red clover to the total sward yield in Year 4, which ranged from 61% for the highest yielding population, AberClaret, to 11% in the lowest yielding, Vivi. Increased red clover DM yield was reflected in a greater CP yield (protein weight per unit area), which ranged from 1.6 t ha−1 year−1 to 2.9 t ha−1 year−1 in Year 2 and from 1.1 t ha−1 year−1 to 1.9 t ha−1 year−1 in Year 4. CP concentration (protein weight per unit herbage weight) of all of the red clover populations was within a range considered suitable for ruminant production. The implication of these results for the future use of red clover in sustainable grassland systems is discussed.  相似文献   

5.
Summary Red clover (Trifolium pratense L.) persistence is improved by the growth of adventitious roots from the crown. The effect of one cycle of divergent phenotypic selection for adventitious roots in red clover was evaluated and root and shoot traits including degree of flowering habit correlated. Three classes of single-cross progenies were produced by crossing plants within and between the high and low root selections. Parents and progeny were grown as spaced plants. Adventitious root score and volume were significantly correlated (p<0.05); however they generally had low or nonsignificant correlations with other variables. A significant correlation (r=-0.36, p<0.01) was found between adventitious root score and flowering score (growth type) in 1988 but not in 1989. Significant variation was found among the three classes of single-cross progeny for adventitious root score and crown diameter. The effect of progeny class on score of adventitious roots was not linear, suggesting dominance effects for the absence of adventitious roots may have been present. A narrow-sense heritability estimate of 0.30 was found by midparent offspring regression of the adventitious root score. This low heritability value implies that successful selection would require progeny testing.  相似文献   

6.
The effects of four row spacings (17.5, 35.0, 52.5 and 70.0 cm) and five seeding rates (50, 100, 200, 400 and 800 viable seeds m?2) on seed yield and some yield components of forage turnip (Brassica rapa L.) were evaluated under rainfed conditions in Bursa, Turkey in the 1998–1999 and 1999–2000 growing seasons. Plant height, stem diameter, pods/terminal raceme, total pods/plant, seeds/pod and primary branches/plant were measured individually. The number of plants per unit area was counted and the lodging rate of the plots was scored. The seed yield and 1000‐seed weight were also determined. Row spacing and seeding rate significantly affected most yield components measured. The number of plants per unit area increased with increasing seeding rate and decreasing row spacing. Plant height was not greatly influenced by row spacing and seeding rate, but higher seeding rates reduced the number of primary branches and the stem diameter. The number of pods/main stem was affected by row spacing and but not by the seeding rate. Also, the number of seeds per pod was not affected by either the row spacing or the seeding rate. In contrast, the number of pods per plant clearly increased with increasing row spacing, but decreased with increasing seeding rate. The plots seeded at narrow row spacings and at high seeding rates were more sensitive to lodging. Seeding rate had no significant effect on seed yield in both years. Seed yield was similar at all seeding rates, averaging 1151 kg ha?1. However, row spacing was associated with seed yield. The highest seed yield (1409 kg ha?1) was obtained for the 35.0‐cm row spacing and 200 seeds m?2 seeding rate combination without serious lodging problems.  相似文献   

7.
Two seed lots of white clover ( Trifolium repens L.) cultivar 'Makibashiro' classified as high and poor quality seed lots, based on the speed of germination (T50), were used to examine the influence of seed quality on field establishment and forage yield. Field emergence rate was estimated approximately three weeks after sowing and found that plots sown with high quality seeds (Lot 1) had 452 seedlings m-2 compared with 392 seedlings in the plots sown with low quality seed lot (Lot 2), but the difference was not significant. However, plant density in autumn (November, 1993), 3 months after sowing, was significantly (P < 0.05) higher in the plots of the Lot 1 seed than that from the Lot 2 seed. The percentage of clover plants surviving over the winter from Lot 1 plots was 55% compared with 39% from the Lot 2 plots, but the difference was not significant. Total forage yield in Lot 1 plots harvested in July and monthly intervals to October, was significantly increased by 44% (P < 0.05) over that of Lot 2 plots, but this greater forage yield in high seed quality seed lot plots was produced early in the season. The results strongly imply that the primary advantage to be gained from high quality seed is an improved field establishment, which led to increased yield of clover. In Lot 1 plots, the percentage of weed dry matter in all the four harvests remained below 1% of die seasonal dry matter production, whereas in Lot 2 plots die percentage of weed dry matter ranged from 3.3–16.9%.  相似文献   

8.
Summary Morphological, isozyme and random amplified polymorphic DNA (RAPD) markers were used to estimate genetic variation within and between cultivars of red clover (Trifolium pratense L.), an important temperate forage legume. Two cultivars of red clover, Essi from Europe and Ottawa from Canada, were evaluated. Six monogenic morphological characters were observed for 80 plants from each of these two cultivars. All six morphological loci were polymorphic in the cultivar Essi whereas only four loci were polymorphic in the cultivar Ottawa. Forty plants from each cultivar were assayed for isozyme markers. A total of 21 enzyme-coding loci with 43 alleles was detected using twelve enzyme systems. Thirteen and nine of these loci were polymorphic in Essi and Ottawa, respectively. The mean number of alleles per locus was 1.81 in Essi and 1.67 in Ottawa. Seventeen random 10-mer primers were screened for RAPD markers. Nine primers which gave clear and consistent amplified products were used to assay 20 individuals from each cultivar. Each primer gave from 7 to 20 amplified bands with an average of 14.8 bands per primer. One hundred and eight of 116 putative loci were polymorphic in Essi and 90 of 98 loci were polymorphic in Ottawa. High within-cultivar variation was observed in both cultivars using both isozyme and RAPD markers. This high polymorphism makes these markers useful for germplasm characterization and genetic studies in red clover.  相似文献   

9.
Molecular marker technologies may provide a tool to overcome the forage yield plateau in timothy (Phleum pratense L.). Therefore, a study was designed to investigate the relationship between marker‐based genetic distance (GD) estimates and yield. Genetic distances were estimated using Dice coefficients framed by 28 simple sequence repeat markers. In Experiment 1, parents with high general combining ability (GCA) from two contrasting first‐generation synthetic strains (exhibiting high and low yields) were compared. Average GDs of each strain were 0.74 (high) and 0.68 (low). These differences indicated that GD may be partially the basis for contrasting yields. In Experiment 2, GDs among 40 parents of a polycross were used for calculating general genetic distance (GGD), which is indicative of their allelic complementation. Analyses revealed a significant (P < 0.01) moderate correlation (r) between GGD and GCA for yield (r = 0.45) and a significant (P < 0.01) residual mean square for the regression of yield on GGD, suggesting that considerable non‐additive effects were associated with GCA. The results are indicative of the potential use of GD estimation for yield improvement in timothy.  相似文献   

10.
In the colder parts of the United States (north of 32° N latitude and east of 92° W longitude), in late winter after disappearance of snow cover, red clover (Trifolium pratense) is often broadcast seeded into forage legume-depleted grass pastures to increase pasture forage quality. This method of establishment is referred to as frost seeding. However, in an estimated 30–40% of frost seeded pastures in Wisconsin, USA the legumes fail to establish. In this study 40 red clover halfsib families from one breeding population and ten check populations were evaluated for spring frost-seeded establishment in three environments. Seedling establishment counts and plant heights were measured 3 months after frost-seeded planting. One of the three environments experienced a stand establishment failure. Narrow sense heritability estimates on a halfsib family basis for stand counts and heights were 0.07 and 0.63, respectively. Seedling counts were greatly affected by environment with micro-environmental effects contributing to low heritability. Additive by environment genetic variance was large, again leading to low seedling count heritabilities. These results, based on one population, suggest that it may be difficult to select for increased frost-seeded seedling establishment and that many test environments are needed to achieve genetic gains for this trait. In comparison, seedling height was very heritable with relatively small additive by environment genetic variances. The genetic correlation between seedling count and height using frost-seeded establishment was r A = 0.42.  相似文献   

11.
Summary The effect of inbreeding on pseudo-self-compatibility (PSC) was investigated in I0, I1, and I2 clones of red clover, Trifolium pratense L., at 40°C. PSC was found to decrease with inbreeding. Significant differences were found among I0 clones, among I1 clones, and among I2 clones for PSC. Significant differences in PSC within generations were found in two of eight I1 clones and one of five I2 clones.A new method of inbred line maintenance is proposed which combines the desirable features of vegetative and seed maintenance of inbred lines. Superior I1 clones with different but homozygous S genotypes and PSC values of 8 to 10% would be vegetatively increased. Clones would be isolated under high temperature field conditions and selfed seed (PSC) would produce the I2. Selfed seed from two different I1 clonal lines would then be mixed and sown to produce single-cross seed. Singlecross seed obtained from different clonal sources would be mixed and sown for the production of double-cross hybrid red clover.Contribution from the Kentucky Agricultural Experiment Station. This paper (72-3-125) is published with approval of the Director, Kentucky Agricultural Experiment Station, Lexington.  相似文献   

12.
Italian ryegrass (Lolium multiflorum Lam.) and perennial ryegrass (L. perenne L.) can be grown for seed and forage in cold winter regions provided the stand persists well over winter. Seed yield and plant characteristics during primary growth, and forage yield during regrowth, were determined for two Italian and one perennial ryegrass cultivars in Atlantic Canada. Establishment methods and dates included sowing ryegrass in cultivated soil alone or with barley in mid‐May and, after harvesting the barley crop, by sowing ryegrass following conventional or reduced cultivation and by no‐till drilling into barley stubble in mid‐August and early September. Despite some winterkill, particularly in Italian ryegrass, seed and forage yields were adequate in post‐establishment growing seasons. Seed yield for Italian ryegrass was greatest (1270 kg ha?1) when it was sown into cultivated soil in mid‐August and least (890 kg ha?1) when sown alone in May. Italian ryegrass yielded 15–17 % more seed when plots were established in mid‐August rather than in mid‐May or early September. Italian ryegrass cv. Lemtal had a greater density of fertile tillers (1030 m?2) in the sward than cv. Ajax (860 m?2) and its tiller density was greater when seeded into cultivated soil in September than in mid‐August. There were fewer spikelets per seed head for sowing Italian ryegrass with barley in May than for the other methods of establishment. Forage yield in regrowth was greater for Italian ryegrass cv. Ajax (2770 kg ha?1) than for cv. Lemtal (2480 kg ha?1). Seed yield of perennial ryegrass was greater when seeded in mid‐May than in mid‐August or early September. The seed yield of perennial ryegrass was greater when it was sown with barley in May and harvested for grain, than when it was sown alone or with barley harvested at late milk stage. The establishment methods for mid‐August and early September sowing had little effect on seed yield. However, the no‐till and reduced tillage methods resulted in a greater tiller density than sowing into the cultivated seedbed. Fertile tillers tended to be denser under reduced cultivation for sowing in August. Forage yield of perennial ryegrass regrowth was not influenced by the sowing method and timing. In conclusion, Italian and perennial ryegrasses produce adequate seed and forage regrowth under different establishment methods and timing. However, the poor persistence of Italian ryegrass may limit commercial production after the establishment year in Atlantic Canada.  相似文献   

13.
Red clover (Trifolium pratense) is an important perennial forage crop that is widely cultivated in Europe. Clover rot remains a major disease in red clover, but resistance breeding is hampered by the lack of available sources of resistance. Moreover, little is known about the factors that influence clover rot resistance. In this paper we evaluated the variation in clover rot susceptibility among a diverse collection of 113 red clover accessions, with the aim of identifying more resistant accessions. Clover rot susceptibility was assessed with a high throughput bio-test on young plants. We found significant variation in clover rot susceptibility, within and among accessions. ‘Tedi’, ‘Maro’ and ‘No 292’ were the most resistant accessions. Fifteen diploid accessions were more susceptible than the average accession with the cultivar ‘Nemaro’ being the most susceptible. Clover rot susceptibility was not correlated with isoflavone levels from Mullaney et al. (Agronomy abstract. ASA, Madison, p 195, 2000). Cultivars were more resistant than landraces and wild accessions and tetraploid cultivars were more resistant than diploid cultivars. Besides the in-depth analysis for clover rot susceptibility, possible correlations with plant architecture and other diseases were investigated. Growth habit, branching, plant yield, flowering date and susceptibility to mildew, virus and rust diseases were investigated in a 3-year field trial. Unlike previously suggested, clover rot susceptibility was not correlated with branching or with plant yield over three years. On the other hand, late flowering accessions and accessions with erect growth habit were less susceptible to clover rot. Clover rot susceptibility was not correlated with susceptibility to rust disease (Uromyces trifolii) or viral diseases, but negatively with susceptibility to mildew (Erysiphe polygoni). Because no completely resistant accessions were found, the best way to improve clover rot resistance would be to select recurrently for resistant genotypes among diverse cultivars and landraces with lower susceptibility. Tetraploidisation of diploid populations with a higher resistance level can provide an additional level of protection.  相似文献   

14.
This study documented the effects of barley companion crop seeding rate and cutting stage on alfalfa establishment in a highland area. Alfalfa was established with barley at seeding rates of 0, 60, 120 and 180 kg ha?1 and cut at the milk‐dough and ripe grain stages. In most cases, hay yield and composition of herbage were affected by companion crop seeding rate and cutting stage. Total hay yield increased from 3294 to 5131 kg ha?1 when the companion crop seeding rate was increased from 0 to 180 kg ha?1 at the milk‐dough stage. Legume and weed growth was suppressed by the companion crop during establishment, but using a barley companion crop decreased alfalfa plant losses in the seeding year. Few residual effects of barley were seen on hay yield in the subsequent year, but residual effects of companion crop treatments on weed suppression continued in all clippings of the second year. The results suggest that alfalfa should be sown with a barley companion crop in highland areas with adequate moisture. The seeding rate for barley is about the same as that for barley grown alone, and the companion crop could be harvested for hay or grain in the establishment year.  相似文献   

15.
The effects of seeding rate (30, 60 and 90 seeds m?2), seeding date (14 January, 28 January and 12 February), seed weight (0.18 and 0.25 g seed?1), seeding depth (3 and 6 cm), and phosphorus fertilization rate (17.5, 35.0 and 52.5 kg P ha?1) and placement method (banded or broadcasted) on field pea (Pisum sativum L.) development and seed yields were investigated in irrigated field experiments conducted in northern Jordan in 2000 and 2001. Results and treatment responses were consistent in both years. Seeding rate, seeding date, seed weight and rate and method of phosphorus fertilization had significant effects on most traits measured; planting depth however did not affect any of the traits. Generally a positive correlation was observed between each factor and seed yield and yield components, with the exception of a negative correlation between seeding rate and yield components, and seeding date and yield and yield components. Increase in seeding rate from 30 to 90 seeds m?2, and increase in P fertilization from 17.5 to 52.5 kg ha?1 alone increased seed yields by 50 and 41 %, respectively. Each delay of 2 weeks for seeding from mid‐January resulted in reductions of 12 % in seed yields. Overall, the results revealed that a combination of early seeding (14 January), of large seeds at an high seeding rate (90 seeds m?2), with P fertilizer banding (52.5 kg P ha?1) maximize field pea yields in irrigated fields in semi‐arid Mediterranean environments. With such management pea seed yields can be as high as 2800 kg ha?1.  相似文献   

16.
Inbred lines of white clover (Trifolium repens L.) have been produced utilising the rare self-fertility (Sf) allele. Twenty-two lines of four distinct groups have been maintained through five generations of selfing by single seed descent. Fourteen lines were used in crosses to produce F1s. Both parents and hybrids were analysed for a range of morphological characters. Positive heterosis for dry matter production was observed in half the hybrids. No other trait showed significant heterosis. The degree of heterosis appears to be related to the extent of variation in morphological characters between the parental lines, some combinations of lines yielding heterotic hybrids in all cases, others in none. The majority of the F1 hybrids are superior in terms of dry matter production to eight control varieties. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

17.
Kura clover (Trifolium ambiguum M.B.) is a persistent rhizomatous forage legume with great potential for permanent pastures. The effects of a soil moisture deficit on forage quality, digestibility and protein fractionation of two cultivars of Kura clover (Endura and Rhizo) were investigated in this study for 1 year. The responses of alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) were also characterized. Stands of each species were field‐grown and submitted to two soil water regimes promoting soil moisture deficits and well‐watered (i.e. control) conditions. There were no interactions between species and soil water regimes. Soil moisture deficit increased acid detergent fibre (ADF) but reduced acid detergent lignin (ADL) content and consequently increased forage digestibility. It had only minor effects on protein content and fractionation. Species varied for most parameters measured. Kura clover generally had the lowest neutral detergent fibre (NDF), ADF and ADL contents, and consequently the greatest digestibility (83.9 %). Species also differed in their crude protein fractionation. Kura clover and red clover had a lower proportion of non‐protein nitrogen (NPN; A fraction) and a greater proportion of true protein (B fraction) (30.9 and 64.5 %, respectively) than alfalfa (36.4 and 57.4 %, respectively). Kura clover also had the lowest proportion (i.e. 4.7 %) of acid detergent insoluble protein (ADIP; C fraction) of all species tested. Endura Kura clover often had a higher forage quality than Rhizo. This study confirms that Kura clover produces high‐quality forage and provides the first estimates of protein fractionation in this species.  相似文献   

18.
Growth and yield of wheat are affected by environmental conditions and can be regulated by sowing time and seeding rate. In this study, three sowing times [winter sowing (first week of September), freezing sowing (last week of October) and spring sowing (last week of April)] at seven seeding rates (325, 375, 425, 475, 525, 575 and 625 seeds m?2) were investigated during the 2002–03 and 2003–04 seasons, in Erzurum (Turkey) dryland conditions, using Kirik facultative wheat. A split‐plot design was used, with sowing times as main plots and seeding rates randomized as subplots. There was a significant year × sowing time interaction for grain yield and kernels per spike. Winter‐sown wheat produced a significantly higher leaf area index, leaf area duration, spikes per square metre, kernel weight and grain yield than freezing‐ and spring‐sown wheat. The optimum time of sowing was winter for the facultative cv. Kirik. Grain yields at freezing and spring sowing were low, which was largely the result of hastened crop development and high temperatures during and after anthesis. Increasing seeding rate up to 525 seeds m?2 increased the spikes per square metre at harvest, resulting in increased grain yield. Seeding rate, however, was not as important as sowing time in maximizing grain yield. Changes in spikes per square metre were the major contributors to the grain‐yield differences observed among sowing times and seeding rates. Yield increases from higher seeding rates were greater at freezing and spring sowing. We recommended that a seeding rate of 525 seeds m?2 be chosen for winter sowing, and 575 seeds m?2 for freezing and spring sowing.  相似文献   

19.
In field trials on a fertile fluvisol in 1995 and 1996 near Göttingen, Germany, pea (Pisum sativum; cv. Messire/conventional leafed, cv. Profi/semileafless) and oats (Avena sativa; cv. Alf) were grown as sole crops and in substitutive mixtures. The sole crops were established at 80 pea seeds m?2 and 300 oat seeds m?2. The mixtures consisted of 67 % (pea) and 33 % (oats) of the monoculture densities, respectively. Interactions of cv. Messire or cv. Profi and oats were similar in 1995 and 1996. The mixtures outyielded the monocultures with respect to total above ground dry matter (RYT = 1.15) and grain yield (RYT = 1.09). Grain yield of pea and oats averaged 4.9 t ha?1 in monocultures and 5.5 t ha?1 in mixtures. Oats was relatively the stronger of the two competitors. Decreasing number of pods per plant could be highlighted as the factor for a lower pea seed frequency in the yield of the mixtures. For oats the number of panicles per plant and kernels per panicle were higher in the mixtures compared with the oat monocultures. The average amount of the harvest index (HI) was 0.52 for pea and 0.46 for oats. Favourable growth conditions increased HI values however, prolific vegetative growth in the mixtures resulted in lower HI values. The predicted RYT‐values estimate the maximum combined grain yield of 6.3 t ha?1 in the mixture of 87 % pea (70 seeds m?2) and 13 % oats (39 kernels m?2).  相似文献   

20.
Summary S allele genotypes of I1 progenies from eight I0 red clover (Trifolium pratense L.) clones were determined under isolated field conditions. Each I1 progeny was vegetatively increased and isolated under a cage for pollination by honey bees. Clones within each I1 progeny producing relatively large and small amounts of seed were classified homozygous and heterozygous, respectively, for S allele genotype. S allele genotypes were verified by extensive sib crosses in the greenhouse, and almost complete agreement was found with the field classification. I2 progenies were reciprocally test-crossed with their parental I0 clones to detect any changes in S specificity and also to confirm the previous S genotype classifications in the I1 generation. It was concluded that the reliability of field and greenhouse sib classification of S genotypes is based on the strength of the incompatibility reaction in each particular clone. Most I1's showed a strong incompatibility reaction as evidenced by low seed set for heterozygous S genotypes, but one progeny showed a weak incompatibility reaction which resulted in S genotype misclassifications. An S specificity was changed in one I2 progeny.Contribution from the Kentucky Agricultural Experiment Station. This paper (72-3-151) is published with the approval of the Director, Kentucky Agricultural Experiment Station, Lexington, Kentucky 40506, USA.  相似文献   

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