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1.
The present case study focused on the problems that affect the nitrification process at three commercial recirculating aquaculture systems (RAS) for salmonids with fixed-bed biofilters operating in Chile, where the main factors were found to be management problems: (1) large variations in daily feeding, which results in unstable nitrogenous compounds (TAN, NO2, NO3) concentration; (2) variable daily water exchange, producing unstable culture conditions (variations in pH and temperature); (3) high densities of culture, which results in overall bad culture conditions (high CO2 concentration, high amount of fine solids, high oxygen consumption). When properly managed, the RAS have proven to tolerate up to 15% of daily variation in feeding, as low as 10% of daily “new” water inlet, and densities as high as 60 kg fish/m3 without showing any nitrification problems. The results from this study demonstrates that maintaining good water quality is essential to secure an efficient growth of both the target species and the nitrifying bacteria, therefore, the production strategies should consider both the target species and the nitrification process requirements.  相似文献   

2.
The present research investigated the effect of carbon/nitrogen ratio (C/N ratio) control in ponds with or without substrate addition for periphyton development on production of giant freshwater prawn. C/N ratios of 10, 15 and 20 were investigated in 40 m− 2 ponds stocked with 2 prawn juveniles (5.023 ± 0.02 g) m− 2 with or without added substrates for periphyton development. The various treatment combinations of C/N ratio and periphyton substrate addition are abbreviated as ‘CN10’, ‘CN15’, ‘CN20’, ‘CN10 + P’, ‘CN15 + P’ and ‘CN20 + P’, P representing periphyton substrate. A locally formulated and prepared feed containing 30% crude protein with C/N ratio10 was applied. Tapioca starch was used as carbohydrate source for manipulating C/N ratio and applied to the water column separately from the feed. Increasing the C/N ratio from 10 to 20 reduced (P < 0.001) the total ammonia-nitrogen (TAN), nitrite–nitrogen (NO2–N) and nitrate–nitrogen (NO3–N) in water column and total Kjeldahl nitrogen (TKN) in sediment. The addition of substrates only influenced the NO2–N concentration in the water column (P < 0.001). Increasing the C/N ratio raised the total heterotrophic bacterial (THB) population in the water column, sediment and periphyton (P < 0.001). It also increased the dry matter (DM), ash free dry matter (AFDM), and chlorophyll a content of periphyton (P < 0.001). The lowest specific growth rate (SGR), the highest food conversion ratio (FCR), and the lowest protein efficiency ratio (PER) were recorded in treatment CN10 (P < 0.05). The addition of substrates did not influence size at harvest (P > 0.05) but improved the survival from 62.8 to 72% (P < 0.001). Increasing the C/N ratio from 10 to 20 increased the net yield by 40% and addition of substrate increased the net yield by 23%. The combination of C/N ratio control and substrate addition increased the net yield by 75% from 309 (CN10) to 540 (CN20 + P) kg ha− 1 (120 days)− 1. This 75% higher production concurred with (1) a lower inorganic nitrogen content in the water column, (2) a higher THB abundance supplying additional single cell protein to augment the prawn production, and (3) an improved periphyton productivity and quality.  相似文献   

3.
The aim of this study was to evaluate the short‐term effect of probiotic inoculation on the abundance of heterotrophic and ammonia‐oxidizing bacteria in mature biofloc, as well as on total suspended solids (TSS), chlorophyll‐a and nitrogenous compounds in water. A completely randomized design consisting of five treatments (three commercial probiotics, one native consortia and one control) was performed. At the beginning of the experiment (day 1), each treatment was inoculated with the respective probiotic: PondPlus® (PP), Efinol® PT (EF) and Epicin® ponds (EP), native consortia UE, whereas the control was not inoculated. Water parameters and bacterial abundance were evaluated at 0, 2, 4, 6 and 8 days. The addition of probiotics, either native or commercial, did not show any significant effect on the TSS, Chl‐a and colony‐forming unit (CFU) of heterotrophic bacteria when they were added to the systems containing mature biofloc. A significant increase in ammonium oxidizing bacteria was registered with the probiotics PP and EP, although the levels of total ammonia nitrogen, NO3‐N and NO2‐N were statistically similar among all treatments. Modifications on most of the parameters measured were associated with the factor of time, rather than the inclusion of probiotics. Results suggest that the bacterial conglomerates in mature stage contain well‐established bacterial communities that are difficult to be affected by the addition of probiotics.  相似文献   

4.
The growth rate of disk abalone, Haliotis discus hannai, energy consumption and changes in water quality were monitored in a pilot-scale recirculating aquaculture system (RAS) for 155 days. Baffles were installed in the RAS culture tanks to enlarge the attachment area and clean out solid waste materials automatically by hydraulic force only. The experimental disk abalones, of shell length 24.5 ± 0.5 mm, were cultured at three stocking densities, 700, 1300 and 1910 individuals/m2 bottom area, in triplicate. The abalones were fed with sea mustard, Undaria pinnatifida, once a week. The abalone feed conversion rates and daily growth rates ranged from 24.5 to 25.9 and 0.32 to 0.36%, respectively. Their daily shell increments and survival rates ranged from 67.7 to 78.6 μm/day and 87.6–92.2%, respectively. The growth in weight tended to decrease at a culture density of 1300 individuals/m2 bottom area, while shell increments and survival rates were acceptable at this density. The total power consumption for heating was 1185.4 kW, comprising 30.2% of the total power consumption, while the average water exchange rate was only 2.9% per day. The total ammonia nitrogen stabilized below 0.07 mg/L, after conditioning of the biofilter. The NO2–N, NO3–N and total suspended solid concentrations were also maintained within acceptable ranges for the normal growth of disk abalone. The use of the RAS with these newly designed culture tanks for disk abalone culture produced 1300 individuals/m2 bottom area with a water exchange rate of only 2.9% per day and used about one-tenth of the heat energy of a conventional flow-through system.  相似文献   

5.
Coral reef fish are collected from the wild and exhibited in aquaria worldwide. Some of the fish spawn in captivity; however, the eggs are usually neglected. In this study, we collected the eggs spawned naturally in the exhibit tanks, hatched and cultured them indoor in 2000‐L fibreglass tanks (initial density = 18 000 egg tank?1). We applied an inorganic fertilization method commonly used in freshwater fish culture in raising these coral reef fish larvae. We maintained inorganic phosphorus concentration at 100 μg P L?1 and inorganic nitrogen at 700 μg N L?1 daily in the fertilized group (n = 4), while the control tanks (n = 4) were fed with rotifers (10 ind mL?1). Chlorophyll a at particle sizes of both 0.45–20 μm and >20 μm, as well as NH3‐N, NO3‐N, and PO4‐P concentrations were significantly higher in the fertilized group than the control. Zooplankton in the size groups of 10–50 μm (mainly flagellates) and 50–100 μm (mainly ciliates) were abundant (about 10~60 ind mL?1) during 3–7 days in fertilized tanks. The average larval fish survival rate at 21 day after hatch in fertilized group was consistently higher than the control in two trials. The experiments demonstrated that the inorganic fertilization approach can be successfully adapted for coral reef fish culture in an aquarium to achieve sustainable exhibits.  相似文献   

6.
Series of trials in which mackerel (Scomber scombrus L.) were confined in keepnets at different stocking densities are described. From simple confinement trials it was found that 50% of the fish died after 48 h at a stocking density of 30 fish m−3, equivalent to 6.5 kg m−3. Trials in which fish were held at stocking densities, and for a duration, comparable to those experienced in a “dried up” purse seine prior to “slipping”, showed that up to 90% of “slipped” fish died within 48 h of release. The primary cause of death was probably physical damage, particularly skin loss, caused by abrasion, although there is some evidence that mackerel have a healing process which can accommodate minor skin abrasions. A tagging trial showed a small but significant increase in mortality due to the tagging procedure.  相似文献   

7.
To study the potential for transmission of Perkinsus karlssoni, an apicomplexan parasite, among bivalve hosts, a long-term laboratory-scale, closed, artificial seawater system for shellfish was designed. Species of shellfish utilised were as follows: bay scallops, Argopecten irradians; blue mussels, Mytilus edulis; eastern oysters, Crassostrea virginica; European oysters, Ostrea edulis; sea scallops, Placopecten megallanicus; softshell clams, Mya arenaria; and quahaugs, Mercenaria mercenaria. All shellfish used were juveniles with shell heights of 10–25 mm. The design was duplicated for control and experimental systems with a combined population of 1600 individual shellfish. The maximum biomass in each system was estimated at < 1 kg/m3. Each system included six, 400 litre rectangular fibreglass tanks, a modular pump and filter unit (particle and activated carbon filters and ultra-violet sterilisers), a biological filter and a refrigeration unit. The total volume of water for each system was 2300 litres of artificial seawater (Instant Ocean®). The mean water temperature of 22°C was achieved by thermostat-controlled room temperature. Salinity was maintained between 27 and 31‰ by addition of either freshwater or artificial seawater. Shellfish were fed daily a mixed diet of carboy-cultured algae and spray-dried algae. Mean values for water quality parameters in both systems were as follows: NH3 < 0·004 mg/litre; NO2 < 0·01 mg/litre; NO3 < 19·16 mg/litre; and pH 8·0–8·4. The system design was adequate in maintaining healthy bivalves for a period of 22 months, with the exception of the sea scallops which succumbed to warm water. Mean monthly shellfish survival rates were 79·9–100% in the control and 74·8–98·9% in the experimental system. A method of controlling water temperature other than via room temperature should reduce slight seasonal temperature fluctuations.  相似文献   

8.
The relative contributions of live Artemia metanauplii and an inert diet for growth of Senegalese sole larvae and postlarvae were assessed through the analysis of carbon stable isotopes ratios (δ13C) in both diets and whole larval tissue. Larvae were reared on four dietary regimes: 100% live prey (rotifers and Artemia), 100% inert formulated diet and two co-feeding regimes of 70:30 and 30:70 ratios of Artemia and inert diet, respectively. Larvae from the live food regime and both co-feeding regimes showed a steep increase in δ13C from 10 days after hatching (DAH) as a result of the onset and continuation of Artemia consumption. From 12 DAH fish larvae from all the regimes showed significant isotopic differences as their δ13C increased to final asymptotic values of − 15.1, − 15.6 and − 16.3‰ in the live food, 70:30 and 30:70 regimes, respectively. Carbon turnover rates in larvae from both live food and co-feeding regimes were relatively high (0.071 to 0.116 d− 1) but more than 90% of the observed change in fish tissue isotopic values was accounted for by the retention of carbon in new tissue growth. A two-source, one-isotope mixing model was applied to estimate the nutritional contribution of Artemia and inert diet to postlarvae growth in the co-feeding regimes. At 23 DAH, the relative contribution of live and inert diets to tissue growth in larvae was respectively, 88 and 12% for the 70:30 co-feeding regime and 73 and 27% for the 30:70 co-feeding regime. At 17 DAH, the estimated proportion of tissue carbon derived from the inert diet was higher at 23 and 38% for the 70:30 and 30:70 regimes, respectively. The results suggest that co-feeding regimes in Solea senegalensis larvae may be adjusted to meet ontogenetic changes in the capacity for larvae to utilise inert diets. The contrasting levels of carbon isotope discrimination between diet and tissue in larvae reared on either 100% live feed or 100% inert diet indicate relatively poor utilization of nutrients from the inert diet. The use of isotopic discrimination factors as potential indicators of the digestive physiological performance of a consuming organism in regards to its diet is discussed.  相似文献   

9.
The lipid classes and the fatty acid (FA) compositions of the zooxanthellae, the host tissue, and intact coral were determined for the first time in a soft coral, Sinularia sp. The contents of monoalkyldiacylglycerol (MADAG), triacylglycerol, and polar lipids differed significantly between the zooxanthellae and the host fractions. The zooxanthellae were rich in polar lipids, whereas neutral lipids were concentrated in the host. MADAG comprised 35% of the host lipids and was practically absent in zooxanthellae. Hence, MADAG is only synthesized in animal tissues and serves as a biomarker for the host in the host–zooxanthellae association of these soft corals. Similar to the zooxanthellae of reef-building corals, the main FA in the zooxanthellae of Sinularia sp. were 18:4n-3, 20:5n-3, and 22:6n-3. In addition, 16:3n-4 and 16:4n-1 (8.9% in total) were found in these zooxanthellae. The ratios of 16:3n-4, 16:4n-1, 18:4n-3, 20:5n-3, and 22:6n-3 in the zooxanthellae to those in the host tissue were 4.2, 11.2, 10.1, 11.0, and 9.1, respectively. The proportions of some FA and lipid classes in the intact coral and its fractions showed that zooxanthellate lipids comprised 36 ± 15% of the total lipids in Sinularia sp. Two tetracosapolyenoic acids (24:5n-6 and 24:6n-3) are proposed as a biomarkers of the animal tissue and indicators of the purity of the zooxanthellae fractions from soft corals.  相似文献   

10.
11.
The effects of feeding frequency (1, 2, 3, or 15 times daily) on oxygen consumption (OC, mg O2 kg−1 h−1) and ammonia excretion, (AE, mg TAN kg−1 h−1) of walleye (Stizostedion vitreum) are described. Walleye were reared at a practical culture density of 35·4 kg m−3 in a single-pass system at 23·2°C. Diurnal variation in metabolic rates were related to feeding, not to photoperiod. Minimum OC rates occurred 30 min before the first feeding of the day, which was the longest average time since the last feeding. Metabolic rates increased immediately after feeding. The maximum rates for OC were 36–49% higher than the minimum rates, and 14–22% higher than the 24-h mean rate. Maximum rates for AE were 137–409% higher than the minimum rates, and 39–87% higher than the mean rates. There was a highly significant difference in the mean metabolic rates related to feeding frequency. The mean OC rate for 1 feeding day−1 (222·0 mg O2 kg−1 h−1) was greater than the mean rates for 2, 3 and 15 feedings day−1. The OC rate for 1 feeding day−1 was 50·6% greater than the rate for 15 feedings day−1 (147·4 mg O2 kg−1 h−1), the lowest mean rate. Mean and maximum oxygen/feed ratio (OFR, kg O2 kg−1 feed fed day−1), varied inversely with feeding frequency. The mean ammonia/feed ratio (AFR) was similar for all but the 3 feedings day−1 treatment, but the maximum AFRs for 2 and 15 feedings day−1 were lower than the AFRs for 1 and 3 feedings day−1. AE was directly proportional to OC; the regression equations were highly significant, but specific for feeding frequency.  相似文献   

12.
The development of standardized and sustainable aquaculture techniques for the cultivation of marine organisms offers many advantages: it would help reduce the quantity of living material harvested from coral reefs; it could be used as an efficient means of rehabilitating impoverished reef ecosystems (e.g. by reseeding depleted natural stocks of corals); it would provide an educational and research tool for the intensive study of marine animal biology under controlled laboratory conditions.Here, we present the results of aquarium maintenance of 3 Red Sea soft coral species (Clavularia hamra, Nephthea sp., Litophyton arboreum) raised from field collected larvae. Planulae settlement was enhanced when dead coral fragments and stones freshly collected from the sea were added to the settlement dishes. Young colonies (n=106, 258, 60; respectively) were monitored for 307, 475 and 207 days, respectively. The survival rate at the end of the observations ranged between 17% and 30%. Growth rates of colonies differed and showed species-specific variations. The most successful growth was recorded in Nephthea where the average colony size reached 324.5 polyps. Current aquaculture techniques for alcyonarian corals not only reveal that these organisms can thrive in aquaria but also provide significantly improved yields of colonies, as compared with yields under field conditions. Our studies have shown that various steps involved in the cultivation of young corals in captivity may need to be specifically tailored to suit the coral species in question.  相似文献   

13.
Satisfying nutrient requirement of corals is still a major constraint for maintaining corals in marine aquariums. Corals are polytrophic in nature. Heterotrophic feeding on zooplankton is one of the corals’ strategies to overcome nutrient deficiency. Artemia salina nauplii are commonly used as biocarriers for many fish larvae in aquaculture and can also serve as a biocarrier for coral in aquariums, provided coral acceptability, optimal feeding rate, and digestibility of the nauplii are well understood. Feeding rate and digestibility of coral fed on A. salina nauplii at 100, 2,000, 4,000, 6,000, and 10,000 ind. l−1 under light and dark conditions was assessed in this study. The maximum feeding rates of Galaxea fascicularis under light and dark conditions was 113.6 ind. polyp−1 h−1 and 76.9 ind. polyp−1 h−1, respectively. The daily feeding rates of G. fascicularis varies and depends on nauplii density. Light plays an important role in coral feeding. Nevertheless, the quantity of A. salina nauplii consumed by the coral under light and dark conditions was not significantly different (P > 0.05). A. salina nauplii are well accepted by G. fascicularis. Complete nauplii digestion was observed after 180 min. Digestibility of A. salina nauplii by G. fascicularis was positively correlated with digestion time.  相似文献   

14.
This paper examines the changes in the population ecology parameters and biomass of golden grey mullet (Liza aurata) in Iranian waters of the Caspian Sea from 1991 to 2005. For most years during this 14-year period, we estimated the age structure of the catch, length–weight relationship, von Bertalanffy growth parameters, condition factor, natural and fishing mortality and biomass. Growth parameters were estimated as L = 62.7 cm, K = 0.15 year−1, t0 = −0.23 year−1. The instantaneous coefficient of natural mortality was estimated as 0.350 year−1 and the instantaneous coefficient of fishing mortality varied during the 14-year period between 0.111 to 0.539 year−1. Biomass estimates of golden grey mullet, from the biomass-based cohort analysis were increased from 13,527 mt in 1991–1992 to 23,992 mt in 2002–2003. In 2004–2005, it was estimated to be 23,658 mt. We concluded that at the present time, the stock of golden grey mullet is not being over-fished.  相似文献   

15.
This study was conducted to determine the biological filtration capabilities of some culinary herbs co‐cultured with lemon fin barb hybrid in a nutrient film technique (NFT) recirculating aquaponic system. Lemon fin barb hybrid (Hypsibarbus wetmorei ♂ × Barbonymus gonionotus ♀) fingerlings were stocked in twelve 2‐tonne fibreglass tanks at 25 fish per tank and co‐cultured with Chinese celery (Apium graveolens var. secalinum Alef.), coriander (Coriandrum sativum) and peppermint (Mentha × piperita) for seven weeks. The impacts of the waste generated by the fish on the water quality, the filtration capability of the herbs and the ability of fish and herbs to retain nutrients (NPK) were also estimated. All the herbs showed water purifying potentials to varying degrees as significantly lower levels of nitrogenous compounds (NH3‐N, NO3‐N, NO2‐N) were observed after the herbal filtration. The plant growth seemed to be affected by their ability to absorb nutrients and consequently purify the culture medium. Interestingly, the lemon fin barb hybrid also showed significant differences in terms of weight gain, but the nutrient retention among fish treatments was not statistically different. The plants absorbed less phosphorus and potassium than the fish. After computing for the total system percentage of NPK recovered, nitrogen was the most retained nutrient. The peppermint showed superiority in terms of gross biomass and water purifying potential compared to the Chinese celery and coriander.  相似文献   

16.
The pink shrimp Farfantepenaeus brasiliensis is native in southern Brazil and is potentially suited for aquaculture. Under intensive culture, the accumulation of nitrogenous compounds results from excretion by the shrimp and from the processes of feed decomposition and nitrification. The objective of this study was to evaluate ammonia, nitrite, and nitrate toxicity effects on oxygen consumption of juvenile pink shrimp. Shrimps (initial weight 0.7 ± 0.15 g) were exposed over a period of 30 days to 50%, 100%, and 200% of the safe levels of total ammonia (TAN = 0.88 mg/L), nitrite (NO2? = 10.59 mg/L), and nitrate (NO3? = 91.20 mg/L) for the species. The specimens were individually collected and placed in respirometry chambers, where the oxygen consumption was measured over a period of two hours. Throughout the experiment there was no significant difference (p > 0.05) among treatments in terms of survival and growth. The pink shrimp juveniles exposed to nitrogen concentrations of 200% of the nitrite and nitrate safe level showed the highest oxygen consumption (p < 0.05).  相似文献   

17.
In this study, the pharmacokinetic profile of enrofloxacin (EF) and its major metabolite, ciprofloxacin (CF), were investigated in brown trout (Salmo trutta fario) (n = 150) after intravenous (i.v.) and oral (p.o.) administrations of a single dose of 10 mg kg− 1 body weight (b.w.) at 10 °C. The plasma concentrations of the drugs were determined by high-performance liquid chromatography (HPLC-UV) from 0.08 to 120 h. Pharmacokinetic parameters were described by the two-compartment open model for intravenous and oral administrations, respectively. After intravenous administration, the elimination half-life (t1/2β), apparent volume of distribution at steady-state (Vss) and total body clearance (Cltot) of enrofloxacin were 19.14 ± 1.51 h, 3.40 ± 0.18 L kg− 1 and 0.14 ± 0.01 L kg h− 1, respectively. After oral administration, the maximum plasma concentration (Cmax), time of maximum concentration (tmax) and bioavailability (F%) were 2.30 ± 0.08 µg mL− 1, 8 h and 78 ± 4%, respectively. Ciprofloxacin was not detected in the present study. The elimination half-life for enrofloxacin following oral administration was longer than values calculated for other animals. After oral administration, the mean plasma concentration was well above the minimum inhibitory concentrations (MICs)—that is, > 0.5 µg mL− 1 at 36 h—for most gram-negative fish pathogens. It is possible and practical to obtain therapeutic blood concentrations of enrofloxacin in brown trout (S. trutta fario) using oral administration of 10 mg kg− 1 body weight; therefore, it may be effective in the therapy for brown trout diseases.  相似文献   

18.
In this study, the toxic effects on the embryos and larvae of the common carp were used as a model to investigate the synthetic pyrethroid pesticide, cypermethrin, which contaminates aquatic ecosystems. Data obtained from the cypermethrin acute toxicity tests were evaluated using the Probit Analysis Statistical Method. The control and eight test experiments were repeated five times. The number of dead embryos significantly increased in response to cypermethrin concentrations 0.0001, 0.001, 0.01, 0.1, 1, 2, 4 and 8 μg l−1 (p<0.05 for each case). The 48 h LC50 value (with 95% confidence limits) of cypermethrin for common carp embryos was estimated at 0.909 (0.256–5.074) μg l−1. Dose–response decreases in hatching success were recorded as 87.4, 85.0, 80.2, 71.4, 56.3, 48.6, 38.8 and 23.5%, respectively. The lowest concentration of cypermethrin (0.0001 μg l−1) produced a significant increase in the number of dead larvae compared to the control group (p<0.05). The number of dead larvae significantly increased with increasing cypermethrin concentrations exposed for 1–96 h (p<0.05). The highest concentration (8 μg l−1) showed the highest larvae mortality. The 96 h LC50 value (with 95% confidence limits) of cypermethrin for common carp larvae was estimated at 0.809 (0.530–1.308) μg l−1. The results of the study suggest that low levels of cypermethrin in the aquatic environment may have a significant effect on the reproduction and development of carp.  相似文献   

19.
This study determined effects of nitrate (NO3?)‐induced stress on faecal cortisol levels in koi (Cyprinus carpio). NO3? was presented in two formats: (1) bulk increase followed by dilution to assess cortisol response and recovery and (2) bulk increase followed by incremental increases to assess cortisol response limit. Fish were maintained group‐wise in treatment and control aquaria (n=6 per group), and 0.5 g L?1 NO3? (as NaNO3) was added to the water. Faecal samples were collected daily and blood samples were taken pretreatment and 72‐h posttreatment and were assayed for cortisol via ELISA. NO3? increased plasma and faecal cortisol 4.4‐fold and 3.9‐fold respectively. Plasma cortisol was not measured further. After a 74% NO3? decrease by dilution, faecal cortisol decreased to baseline within 24 h, and restimulation by NO3? (1.0 g L?1) elevated faecal cortisol to maximal study levels. In a separate experiment, exposure to 0.4 g L?1 NO3? increased faecal cortisol 6.9‐fold. However, three additional 0.4 g L?1 NO3? increments across 9 days did not further increase cortisol. This study demonstrates that faecal cortisol measurement in fish via an ELISA can be useful as an indicator of NO3?‐induced stress in the aquatic environment.  相似文献   

20.
Two experiments were conducted to investigate the effects of varying dietary protein (35–45%) and energy levels (17.34–19.44 kJ g−1) on the growth performance of milkfish (Chanos chanos) maintained under laboratory (experiment 1) and field conditions (Experiment 2) in inland saline groundwater. The results of experiment 1 (initial weight of fish: 0.25 g) revealed that, irrespective of the protein source (fish meal or processed full fat soybean), fish fed a diet containing 40% protein showed significantly (p<0.05) higher growth in terms of live weight gain and specific growth rate, low feed conversion ratio [1.71 (fish meal) and 1.58 (soybean)], high nutrient retention (gross protein retention: 28.59 and 31.05%; gross energy retention: 24.23 and 26.04%), apparent protein digestibility (81.74 and 85.91%) and digestive enzyme activity (specific protease and amylase). An observation on the postprandial excretion of metabolites (N–NH4 and o–PO4) indicated significantly (p<0.05) low levels of these metabolites in aquaria where the fish were fed 40% dietary protein irrespective of the protein source. The results of experiment 2 also indicate (initial weight of fish: 4.43 g) a significantly (p<0.05) higher growth performance (weight gain, biomass, specific growth rate, growth day−1 and fish production) in ponds where the fish were fed 40% dietary protein. Irrespective of the experimental conditions (laboratory/field), feeding the fish higher dietary protein levels (beyond 40%) not only repressed growth performance but also affected proximate composition by lowering protein accumulation and energy assimilation. An investigation on the effects of feeds on water quality parameters revealed that values for total alkalinity, NH4–N, NO3–N, turbidity, total dissolved solids and parameters indicative of productivity (chlorophyll a, net primary productivity and plankton population) increased significantly (p<0.05) from treatments 1–3 (35–40% protein) and declined thereafter, in treatment 4 (42% dietary protein), indicating that water quality characteristics correlated well with fish growth. The results suggest that in order to obtain a high-yield in milkfish culture system the fish should be fed supplementary diets containing appropriate (40%) protein levels, especially when the stocking rates are high.  相似文献   

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