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1.
Four bovine leukemia virus (BLV)-seropositive and 2 BLV-seronegative cows were used as donors in a study to provide evidence whether IM injection with common needles is a means of spreading bovine leukemia. Sheep were used as recipients. Of the 4 BLV-seropositive cows, 2 had high virus expression (VE; 43% and 28% of their lymphocyte thin sections had associated BLV-particles), whereas the other 2 cows did not have observed VE. After each of the 4 cows was given an injection of a 5-antigen Leptospira bacterin, a BLV-seronegative sheep was immediately given an injection of the same bacterin with the same needle. None of these sheep seroconverted, nor did either of 2 sheep given only the bacterin (with a previously unused needle). Sheep inoculated IM with 0.2 ml of whole blood from both of the cows with high VE and from 1 of the 2 BLV-seropositive cows that did not have observed VE did seroconvert. In contrast, the sheep inoculated with 0.2 ml of blood from the remaining BLV-seropositive (0% VE) cow and from the 2 BLV-seronegative cows remained seronegative. These results were interpreted to indicate that the quantity of infective lymphocytes passed during injection with common needles is too small to induce infection.  相似文献   

2.
Serological survey of bovine immunodeficiency virus (BIV) and bovine leukemia virus (BLV) infection was conducted in dairy cattle from 10 different regions of Hokkaido, Japan. Among 390 cattle, 11.0% of cattle were BIV-seropositive and 3.3% were BLV-seropositive. Moreover, in two dairy farms, where bovine leukosis has been reported, prevalence of BIV infections were 6.4 and 9.1%, respectively. In contrast, among 150 beef cattle, 16.6% were BIV-seropositive while none was BLV-seropositive. Dual infections with BLV and BIV in dairy cattle were tested by using 107 BLV-seropositive sera, and 20 sera were found BIV-positive (18.7%). These results indicate that BIV infection was widespread in Hokkaido.  相似文献   

3.
To determine the prevalence of single vs. dual infection with bovine immunodeficiency virus (BIV) and bovine leukemia virus (BLV), sera (n = 95) from a dairy cattle herd were analyzed for anti-BIV and anti-BLV antibodies by an enzyme linked immunosorbent assay. Twenty-one percent (20/95) of samples were BIV-seropositive, while 52% (49/95) of the same samples were BLV-seropositive. A significantly greater percentage of BIV-seronegative samples were BLV-seropositive, 57% (43/75), than were BIV-seropositive samples, 30% (6/20). There was no significant correlation between data ranked from least to greatest amount of anti-viral antibody. Five cattle had persistent lymphocytosis (PL); all five were BLV-seropositive and two were BIV-positive. The mean anti-BLV titer was significantly greater in PL cattle, as compared at non-PL cattle, whereas there was no significant difference between the mean anti-BIV titer in PL cattle, as compared with non-PL cattle. These results provide additional information on the seroprevalence of naturally occurring BIV infection, and indicate that BIV can exist independent of other common infectious agents, such as BLV. Further, the results suggest that infection with BIV is not associated with an increased rate of infection with other infectious agents such as BLV.  相似文献   

4.
Before 1985 the situation regarding enzootic bovine leukosis (EBL) in Lithuanian cattle was described only haphazardly. In 1986 serological investigations were initiated together with an eradication programme. The EBL bovine leukosis virus (BLV) situation was monitored by the Institute of Immunology Vilnius University, national and regional veterinary laboratories. Starting in 1986 all EBL-positive cattle were separated from negative cattle into BLV-infected and BLV-free herds. To create the latter, calves were fed pasteurized milk. The seroprevalence in 1990 was 7.29%, but it steadily declined to 0.32% in 2006.  相似文献   

5.
Blood samples were obtained from sentinel beef cattle at monthly intervals, and the sera were tested for antibodies, using a bluetongue virus (BTV) immunodiffusion test (IDT) and virus-neutralization test (VNT), for 5 BTV serotypes (2, 10, 11, 13, and 17) and 2 epizootic hemorrhagic disease virus (EHDV) serotypes (1 and 2). The cattle tested were transported from Tennessee to Texas in 1984 and 1985. All cattle were seronegative by the BTV IDT at the initial bleeding in Texas in 1984 and 1985. In 1984, 16 of 40 (40%) cattle seroconverted as assessed by results of the BTV IDT. In the 16 seropositive cattle in 1984, neutralizing antibodies were detected to BTV serotypes 10 (n = 7), 11 (n = 3), and 17 (n = 11), and EHDV serotypes 1 (n = 1) and 2 (n = 7). In 1984, no cattle seroconverted to BTV-2 or BTV-13. In 1985, 10 of 36 (27.8%) cattle seroconverted as assessed by results of the IDT. Of the 10 seropositive cattle in 1985, neutralizing antibodies were detected to BTV serotypes 10 (n = 10), 11 (n = 10), 13 (n = 7), and 17 (n = 5), and EHDV serotypes 1 (n = 1) and 2 (n = 7). In 1985, no cattle seroconverted to BTV-2. Clinical diseases attributable to BTV or EHDV was not detected in these cattle in 1984 or 1985.  相似文献   

6.
Experiments were conducted in 1985 and 1986 at the Eastern Ohio Resource Development Center, Belle Valley, to examine the feasibility of using embryo transfer to induce twinning and to examine the influence of twinning on traits of the cow and calf. Embryos were collected from a total of 14 superovulated Angus donors on two dates each in 1985 and 1986 and were transferred to Angus recipients. A total of 124 embryos were transferred to 79 recipients, with 43 (34.7%) calves born alive. Seven of 45 (15.6%) recipients implanted with two embryos produced twins. In no case did both halves of the 15 embryos that were split to produce identical twins and implanted in the same recipient survive to birth. Proportion of calves born alive did not differ among transfer codes 3 (nonsplit embryos from two different donors implanted in separate uterine horns of the same recipient), 6 (nonsplit embryos from one embryo flush implanted in separate uterine horns of the same recipient) and 7 (nonsplit embryos from two different donors implanted in the same uterine horn of one recipient). Surgical transfers tended to result in a higher proportion of embryos surviving to birth (.43 vs .21; P = .16) and a higher twinning rate (.29 vs .04; P = .36) than did nonsurgical transfers. Age of recipient did not influence embryo survival (P = .98) or twinning rate (P = .99). Gestation length was 5 d shorter (P less than .01) for twin calves than for singles. Singles were 9 kg heavier (P less than .01) at birth and 32 kg heavier (P less than .01) at weaning than twins. However, cows raising twins produced 108 kg (51%) more total weaning weight than did cows raising singles.  相似文献   

7.
1986年以来,以南阳牛为母本,引进意大利皮埃蒙特牛为父本,通过杂交创新、横交固定、世代选育培育皮南牛(外血75%)。采用常规育种与全基因组选择选育相结合,实现了精准选育,提高了选择效率。培育的皮南牛屠宰率、净肉率、眼肌面积大等优点,已基本具备新品种申报条件。  相似文献   

8.
Breeding trials were designed to determine the influence of the mare on serum concentrations of equine chorionic gonadotropin (eCG) from Day 39 to Day 104 of gestation. Sires were ranked according to mean eCG concentrations found in the groups of randomly selected mares to which they were mated in 1983. Mares were ranked according to their mean eCG concentrations on Days 55, 71 and 85 of gestation (Day 0 = mating), in 1983 and 1985. In the 1986 breeding season, mares that had pregnancies characterized by high eCG levels were mated to sires previously associated with low eCG concentration pregnancies and low producing mares were mated to sires associated with high eCG concentration pregnancies. The highest eCG concentrations (12.8 ug/ml serum) were detected on Day 55 of gestation in mares mated to the low-ranked sire (P<0.01), indicating an influence of the mare on serum eCG concentration. A comparison of eCG production by individual mares in 1985, 1986 and 1987 showed that mares retained the same rank (P<0.001), regardless of the rank of the sire to which they were mated. It was concluded that the influence of the mare predominates in determining eCG concentration. In 1987, the highest ranked sire was mated to the highest eCG producing mares (ranked 1–20) and the second highest ranked sire was mated to mares ranked 21–40. Two distinct subgroups resulted, suggesting that the selection of sires and mares could be used to maximize the production of eCG.  相似文献   

9.
A study was conducted to examine the usefulness of a glycoprotein I (gI)-ELISA to monitor Aujeszky's disease virus infection in two vaccinated pig herds; the gI-ELISA can differentiate between pigs infected with Aujeszky's disease virus and pigs vaccinated against Aujeszky's disease with gI-negative vaccines. The two herds had been vaccinated with gI-negative vaccines for several years. The first survey, in September 1986, revealed that approximately 10 per cent of the breeding pigs in a large multiplier herd were seropositive for antibodies to gI of Aujeszky's disease virus, and it was decided to try to eliminate the virus from the herd by gI-ELISA testing and culling of gI-seropositive pigs. A one month quarantine period for incoming stock was established, and only gI-seronegative pigs were admitted to the herd. After two rounds of testing and culling the herd appeared to be free of wild-type Aujeszky's disease virus, and neither Aujeszky's disease virus nor antibodies could be detected either in 21 sentinel pigs placed on the farm or in 347 stillborn piglets or piglets that died shortly after birth. The herd probably remained free of Aujeszky's disease virus until the end of the 27-month period of monitoring except for two of 639 breeding pigs that were unexpectedly found to be positive in the gI-ELISA in November 1987. These sows were culled. A second breeding herd was monitored for antibodies to gI of Aujeszky's disease virus for two years. The gI-seropositive sows constituted approximately 30 per cent of the herd's breeding pigs, but they were not culled.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

10.
内蒙古三河牛是我国自主培育的乳肉兼用型品种,它是呼伦贝尔各族人民一百多年来辛勤劳动的结晶。从1986年育成以来,三河牛以核心家系选育的方式延续严格的保种制度,在呼伦贝尔草原严酷环境中保持良好的生产性能和繁殖力。虽然在近20年不断受到荷斯坦牛等高产品种的挑战,但对当地气候的高度适应性和良好的兼用性让三河牛始终立于不败之地。"十一五"以来,三河牛选育进一步得到国家重视,在原有品种登记和性能测定系统的基础上,修定并发布了新的三河牛品种国家标准,建立了三河牛育种信息数据库,引入奶牛DHI测定体系,强调兼用性的育种目标,吸收国外兼用型品种高水平遗传种质,采用开放式核心群育种体系,不断取得各方面进展。现简述三河牛培育历程,并总结目前的各项进展,与业界同仁分享。  相似文献   

11.
奶牛良种是产业发展的基础,也是陕西奶牛发展的短板。为破解制约良种繁育存在的问题,本研究对占全省奶牛存栏95%以上的34个规模化奶牛场进行调研,从良种的定义与现状、良种繁育的历史与现状、存在的问题及对策与建议等方面进行分析,以期为陕西省奶牛良种繁育工作提供参考借鉴,助推我省奶牛产业健康持续发展。  相似文献   

12.
南阳牛是我国五大良种黄牛品种之一,近些年国家越来越重视通过杂交改良的方法产生新品种培育工作,1986年引进了皮埃蒙特牛,与南阳牛进行杂交改良,通过30多年来的持续选育,横交固定形成了皮南牛新类群,它保持了南阳牛适应性好、耐粗饲的优点,同时继承了皮埃蒙特牛生长发育快、瘦肉率高的特点,但在皮南牛新品种培育过程中,仍存在核心...  相似文献   

13.
A cross‐sectional study was carried out on sheep and cattle slaughtered at a New Zealand abattoir from September to November 2010 to investigate the supplier‐specific shedding rate, renal carriage rate and seroprevalence of leptospires. In the 2008/2009 season, this abattoir experienced three human leptospirosis cases from 20 staff, of which two were hospitalized. Urine, kidney and blood samples were collected from carcasses of 399 sheep (six suppliers, 17 slaughter lines) and 146 cattle (three suppliers, 22 slaughter lines). The urine and kidney samples were tested by quantitative real‐time PCR (qPCR), while serum samples (from coagulated blood samples) were tested by microscopic agglutination test (MAT). In total, 27% (73/274; 95% CI: 18–37) of urine samples tested positive by qPCR. Species‐specific shedding rates (prevalence of positive urine qPCR) were 31% (95% CI: 17–48) for sheep and 21% (95% CI: 14–30) for cattle. For 545 kidney samples tested, 145 were qPCR positive (27%; 95% CI: 17–39). The average prevalence of kidney qPCR positivity was 29% (95% CI: 17–45) for sheep and 21% (95% CI: 15–28) for cattle. Three hundred and thirty of 542 sampled sheep and cattle had antibodies against Leptospira borgpetersenii serovar Hardjobovis (Hardjobovis) and/or Leptospira interrogans serovar Pomona (Pomona), based on reciprocal MAT titre ≥1 : 48 (overall seroprevalence of 61%; 95% CI: 48–73). Seroprevalence was 57% (95% CI: 40–72) for sheep and 73% (95% CI: 59–83) for cattle. Among the seropositive animals, 41% (70/170; 95% CI: 30–54) were shedding (tested positive by urine qPCR) and 42% (137/330; 95% CI: 30–54) had renal carriage (tested positive by kidney qPCR). Some risk management options for abattoirs or farms to prevent human leptospirosis infections include vaccination of maintenance hosts, the use of personal protective equipment, and the application of urine qPCR to detect shedding status of stock as surveillance and as an alert.  相似文献   

14.
We conducted a seroepidemiological survey for antibodies to bovine leukemia virus (BLV), by using agar gel immunodiffusion technique, in dairy cows, water buffaloes, and yellow cattle throughout Taiwan. The positive reactors were 8.4% (376/4,459) in 1985 and 5.8% (1,277/22,190) in 1986, in 15 prefectures and 7 cities. Relatively high infection rate appeared in the northern and southern areas of Taiwan. Positive reactors increased gradually with age. The incidence of positive antibodies was 2 to 3 times higher in pasture-style farms than in housed-style farms. Among the 6,313 imported cattle, 302 (4.8%) showed positive reaction. Between 1985 and 1987, 5 cattle showed enzootic bovine leukosis among 351 sero-positive reactors in four highly positive prefectures. Survey of 134 water buffaloes and yellow cattle showed no positive reactors. This survey demonstrated that BLV-infection has increased over the years and spread throughout Taiwan.  相似文献   

15.
西门塔尔牛为乳肉兼用品种。是世界第二大牛品种,全世界现有存栏数为4100多万头。内蒙古是中国西门塔尔牛品种培育基地,西门塔尔牛已成为该地区肉牛产业的主导品种。2013年末,内蒙古肉牛存栏570万头.其中90%以上的良种与改良种为西f1塔尔血统。在对西门塔尔牛生产特性进行详细分析的基础上,提出了养好西门塔尔牛的技术措施,以期为内蒙古地区的西门塔尔牛养殖提供参考。  相似文献   

16.
Two trials were conducted with ewes to determine the effects of prostaglandin F2 alpha (PGF) administration during the first week of gestation. In trial 1, ewes (n = 134) were checked for breeding activity once daily and half of them received 10 mg PGF im at either 0, 24, 48, 72, 96, 120 or 144 h after detection of a breeding mark. The other half served as uninjected controls. In trial 2, ewes (n = 153) were checked for breeding activity twice daily. Two-thirds of the ewes received 10 mg PGF at either 24, 36, 48, 60, 72, 84, 96, 108, 120 and 132 h following detection of a breeding mark. The other one-third of the ewes served as uninjected controls corresponding to treatment times of 24, 48, 72, 96 or 120 h. In trial 1, the percentage of ewes lambing as a result of first service decreased as time of administration of PGF increased. The first-service pregnancy rate was 87.5% for ewes given PGF at 0 h and 0% for ewes given PGF at 144 h. Fewer (P less than .05) ewes given PGF at 96, 120 or 144 h after first mating lambed than control ewes. Similarly in trial 2, fewer (P less than .05) ewes given PGF at 96, 108, 120 or 132 h after first mating lambed than did controls. The total number of ewes lambing as a result of the entire breeding season did not differ (P greater than .05) between treated and control ewes in trial 1 (88.2 vs 87.3%) or trial 2 (85.7 vs 83.3%).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

17.
SUMMARY The efficiency of 2 different doses of bovine PPD tuberculin was compared using the caudal fold test for the detection of tuberculosis in beef cattle. Two matched groups of 98 cattle were selected on the basis of their reactivity to HCSM tuberculin. Cattle in each group were tested with a single 0.1 ml dose of bovine PPD tuberculin containing either 0.1 mg or 0.2 mg bovine PPD respectively. Two further groups of 100 young stock from a herd with an incidence of tuberculosis of less than 0.1% were selected as controls. Tests were interpreted subjectively by palpation and observation and objectively by caliper measurement at 48, 72 and 96 h. All cattle were examined post mortem for the presence of visible lesions.  相似文献   

18.
In Switzerland domestic cattle (Bos primigenius f. taurus resp. Bos taurus L.) first appear with the earliest Neolithic settlements (approximately 5000 BC). With the gradual deforestation of the landscape caused by human exploitation of the environment, cattle were used more intensive and in many ways. There is evidence that cattle were used as draught animal since ca. 3400 BC, probably even earlier milk was regularly used. The size of domestic cattle gradually decreased from Early Neolithic until Iron Age. Only with Roman influence larger animals are found. However, after the withdrawal of Romans the average size of cattle decreased again. Archaeogenetic studies will have to show, whether this is due to novel breeding strategies or the import of breeding stock. First genetic results showed that a female genetic type, which is rare in European breeds, is present in Swiss Evolène cattle and in one animal of Roman time cattle from Augusta Raurica. Is this a sign for influence of Roman cattle on today's Swiss breeds?  相似文献   

19.
Cattle's grazing of the locoweed Wahweap milkvetch (Astragalus lentiginosus var. wahweapensis) was evaluated on desert and foothill winter range during the winter of 1986 to 1987. Dry, dead stalks of Wahweap milkvetch that had grown in 1985 and 1986 made up 15% of cattle diets overall, and 24% of diets when cattle grazed gravelly benches where it was abundant. Nutritional quality of Wahweap milkvetch was higher than of most associated forage, but its alkaloid concentration varied among the senescent stages. One cow aborted and two cows developed clinical signs of locoweed poisoning, including water belly (hydrops amnii). Microscopic lesions consisting of cytoplasmic foamy vacuolation were evident in both the dam and fetus. Because senescent stalks of Wahweap milkvetch are palatable and readily grazed by cattle, ranchers should not permit cattle to graze infested sites until other green forage is available in the spring.  相似文献   

20.
夏南牛新品种培育技术研究   总被引:1,自引:0,他引:1  
夏南牛培育项目始于1986年。夏南牛是以夏洛来牛为父本,以南阳牛为母本,应用人工授精技术,采用杂交创新、横交固定和自群繁育三阶段开放式育种方法,根据血统、外貌、体重综合选择,以体重为主,同时考虑早熟性,经过21年严格的选种选育,育成的含夏洛来牛血37.5%的肉用牛新品种。通过中间试验、育肥试验、屠宰试验,夏南牛表现出良好的肉用特征,生长发育速度、屠宰率、净肉率、肉品质等方面明显优于南阳牛。本文总结出了一套适合我国国情的肉牛新品种育种成功经验。  相似文献   

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