共查询到19条相似文献，搜索用时 218 毫秒
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为充分了解及更合理地利用无核葡萄的种质资源,辅助无核葡萄育种,本研究采用SRAP分子标记技术,对23 个国内外主流无核葡萄品种的种质亲缘关系进行分析。结果表明：从45 对SRAP引物中筛选出38 对多态性引物,共扩增出236 条谱带,其中171 条为多态性谱带,多态性谱带百分率为72.4%。遗传相似分析显示,供试材料间遗传相似系数的变化范围为0.58~0.90,遗传距离在1.0313~4.9643 范围内变化。在遗传相似系数为0.61 处,可将供试材料分为2 个大类群,在相似系数为0.66 处,Ⅰ类群和Ⅱ类群均可分为2个亚群。说明23个品种总体来讲亲缘关系较远,特别欧美杂种具有较高的遗传多样性,部分欧亚种品种间亲缘关系较近,杂交育种时应注意亲本选择。 相似文献
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我国39个茶树品种的RAPD分析 总被引：6，自引：1，他引：5
在前人研究的基础上,本研究结合茶树特点,进行综合设计,探索出一套操作简便、快速、准确的高质量茶树DNA提取和纯化技术。从100条RAPD随机引物中筛选出20条具有多态性的引物,对这39份材料进行RAPD分析,扩增共产生196条RAPD标记带,其中多态性带171条,多态性百分率为87．2％。依据RAPD分析结果,通过UPGMA类平均法聚类法,对供试的39个茶树品种进行聚类分析,绘制出基因组DNA之间的遗传关系树状图,结果表明：它们之间的遗传相似系数在0．17-42．97之间。揭示了不同省（区）地方性的茶树品种之间遗传多样性、遗传相似性与亲缘关系,通过分析证实了这些品种间存在着亲缘关系,进一步明晰了彼此之间的遗传距离。 相似文献
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30个香蕉品种遗传多样性的ISSR分析 总被引：11，自引：0，他引：11
利用ISSR技术对香蕉30个品种的遗传多态性进行了分析。从60个随机引物中筛选出8个多态性引物,共扩增出54条DNA带,其中44条为多态性带,占81.5%,平均每个引物扩增的DNA带数为6.75条。按照相同迁移位上有扩增带记为1、无带为0的方法构建ISSR表型数据矩阵,用MEGA2.0软件对香蕉30个品种进行UPGMA聚类分析,计算出30个品种间的平均距离系数为0.313,并在此基础上建立了聚类分析树状图,将香蕉30个品种划分为A、B、C、D4大聚类组。 相似文献
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龙眼种质资源的RAPD分析 总被引：3，自引：0，他引：3
利用RAPD技术对龙眼的95份种质资源和一份荔枝种质资源进行研究。从200条随机引物中筛选出18条多态性引物,共扩增出197条带,DNA扩增带的多态性为100%,这表明96份材料的遗传多样性和遗传资源的丰富性。利用SPSS13.0统计软件对RAPD表型数据矩阵进行分析。根据欧氏距离（ED）法计算各样品间的遗传距离,采用UPGMA（类平均法）,进行分析,建立亲缘关系聚类树状图。结果表明,RAPD标记能在分子水平揭示龙眼的遗传背景与亲缘关系 相似文献
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【研究目的】为了解花椰菜种质资源的遗传多样性和亲缘关系,为花椰菜育种提供理论基础,本研究采用RAPD技术对37份花椰菜种质资源的遗传多样性进行了分析;【结果】从80条随机引物中筛选出6条随机引物,6条引物扩增获得的总谱带数为38条,多态性谱带数为13条,多态性谱带比例为34.2%,利用随机引物S17对花椰菜的遗传多样性进行分析,发现当遗传距离为0.41时,可将花椰菜种质资源分为4类;【结论】花椰菜的遗传多样性与地理种质资源的地理来源及生育期关系不大,部分不同地区的品种相互交织在一起,这可能是由于地区间的品种互引造成的,有些品种地理近源但亲缘关系较远,有些品种地理远源但亲缘较近。 相似文献
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《种子》2018,(12)
利用ISSR技术对14个八仙花品种进行了亲缘关系的研究。从49个引物(引物选自加拿大哥伦比亚大学UBC公司公布的100条引物)中筛选了8个条带清晰、多态性高、重复性好的引物,对14个八仙花品种DNA进行扩增,共扩增出478条带(平均每个引物扩增59.75条DNA片段),多态性条带420条。用POPGENE分析数据得到14个品种的遗传距离为0.000 2～0.087 4,遗传相似性系数为0.916 3～1.000 0;利用NTSYSpc 2.1聚类分析,在遗传相似性系数约为0.38处将14个八仙花品种明显分为两大类,其中花园蕾丝、绿色夏天和圣代草莓被聚为第一类,其余11个品种被聚为第二类。 相似文献
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荔枝种质遗传多样性的SRAP分析 总被引：8，自引：0，他引：8
为评价荔枝种质的遗传多样性,从88对SRAP引物中筛选出22对多态性高、稳定性好的引物,用于研究46个荔枝品种.每对引物组合产生6～19条谱带,其中多态性条带284条,占总带数的97.93%.46个种质的相似系数在0.469～0.838之间,平均相似系数为0.644.其中,秤砣与红荔的相似性系数最大,亲缘关系最近;小丁香与三月红、三月红与A13两对相似系数最小,亲缘关系最远.UPGMA聚类分析显示,在相似系数为0.644处46份荔枝供试品种被划分为4个类群,并且来源地相同的品种间亲缘关系较密切.22对多态性引物中,引物me7-em2在海垦18中扩增出1条特征谱带,引物me3-em7在水东中也扩增出1条特征谱带,而仅凭这1条特征带就可以将荔枝品种海垦18或水东与其它材料区别开来.这说明SRAP标记可用于荔枝的品种鉴定和群体遗传结构的研究. 相似文献
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Prasert Kongkiatngam Marcia J. Waterway Bruce E. Coulman Marc G. Fortin 《Euphytica》1996,89(3):355-361
Summary The use of random amplified polymorphic DNA (RAPD) markers obtained from bulked samples was investigated for cultivar identification in red clover. Pooled samples were examined in order to minimize variation within cultivars. To determine the appropriate number of individuals to include in the bulked samples representing each cultivar, DNA samples from two, three, four, five, ten and twenty individuals were pooled. Twenty was found to be an appropriate number of red clover individuals per bulk in order to amplify only the DNA sequences shared among most individuals in each cultivar. Fourteen 10-mer primers were used to amplify genomic DNA from combined leaf samples of 15 red clover cultivars from European, Japanese and North American origins. A total of 79 amplified products, of which 55 were polymorphic, was obtained. Cultivar-specific bands were observed with 13 primers. The amplification patterns obtained from two primers could distinguish all 15 red clover cultivars. Rogers' genetic distances for all 105 pairwise comparisons were calculated to evaluate relationships among these cultivars. Cluster analysis based on these genetic distances separated these 15 cultivars into three groups, with two of the groups consisting of a single Japanese cultivar each, while the third group included cultivars from European, North American, and Japanese origins. 相似文献
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DNA from thirty-six cymbidium cultivars was examined using polymerase chain reaction (PCR) to determine the efficiency of
randomly amplified polymorphic DNA (RAPD) markers in identifying cultivars and determining levels of genetic variability.
A total of 132 RAPD markers, 78% of which were polymorphic, were produced from 15 10mer arbitrary primers. All the cultivars
were distinguishable when a number of primers was considered. One cultivar, Blue Smoke ‘Green Meadow’ could be distinguished
from all the rest based only on lack of the OPA5-370 fragment. Genetic distances among the cultivars were estimated based
on the amount of band sharing and ranged from 0.08–0.50 with an average of 0.29. Cluster analysis of genetic distance estimates
grouped siblings together with each other and parents with offsprings, thereby agreeing with known parentage information and
corroborating isozyme data obtained from a separate study. The possible application of the observed polymorphism and variation
to cymbidium breeding is discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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DNA polymorphism among nine cultivars of Asparagus officinalis L. was measured using random amplified polymorphic DNA (RAPD). Of 69 reproducible amplification products from 12 arbitrary decamer primers, 49 RAPD markers were polymorphic and could be used to distinguish six German and three Dutch asparagus cultivars. Even with very small sample sizes, genetic similarity measurements based on the RAPD data allowed accurate grouping of the nine cultivars into distinct clusters, with the exception of two individuals which clustered to closely related varieties. Two German cultivars showed high genetic similarity and were distinct from the remaining German varieties. The German and Dutch cultivars were clearly separated by a relatively large genetic distance. 相似文献
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Genetic diversity of hexaploid wheat cultivars estimated by RAPD markers, morphological traits and coefficients of parentage 总被引：3，自引：0，他引：3
S. Mari&#; S. Bolari&#; J. Martin&#;i&#; I. Peji&#; V. Kozumplik 《Plant Breeding》2004,123(4):366-369
Estimates of genetic diversity can be based on different types of data. The aim of this research were to study genetic diversity among Croatian wheat cultivars by random amplified polymorphic DNA (RAPD) markers, morphological traits and pedigree records; to analyse differences between wheat cultivars from two breeding centres; and to evaluate usability of RAPD markers for estimation of genetic diversity among wheat cultivars in comparison with morphological traits and pedigree record data. Studies were conducted on 14 wheat cultivars and breeding lines from two breeding centres in Croatia. For the RAPD analysis 36 primers were screened and the 14 most polymorphic ones yielded 341 polymorphic bands. Twelve morphological traits were used for morphological analysis. Pedigrees were composed of seven generations of ancestors. RAPD markers showed a high level of polymorphism among the cultivars examined and the breeding lines. No significant correlations were observed among the methods tested. 相似文献
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白菜种质遗传多样性与亲缘关系的ISSR标记分析 总被引：1，自引：0，他引：1
摘要：从分子水平用ISSR标记法对白菜遗传多样性进行分析,从100个ISSR引物中共筛选出11个多态性明显、条带清晰、反应稳定的引物,对65个样品DNA共扩增出107条谱带,平均每个引物扩增出9.72条带,其中多态性位点102个(93.5% )。种间遗传相似系数在遗传距离在0.40～0.65 之间,表明白菜栽培种内品种间的遗传基础相对较宽,存在较大的遗传变异性。利用UPGMA聚类分析表明：能将65个白菜地方品种划分为四大类。由ISSR标记聚类结果所表现出的大多种质之间的亲缘关系与其来源地有较大的相关性,但也有地理差别很大的白菜资源遗传关系较近的情况。本研究还表明,ISSR 标记比RAPD 标记具有更高的稳定性,在植物遗传多样性的分子标记或克隆研究中,可优先使用ISSR 标记。 相似文献
16.
Summary DNA polymorphism among five Asparagus officinalis L. cultivars-Imperial, Snow, Steline, UC-157 and Larac, as detected by random amplified polymorphic DNA (RAPD), is reported. Thirty one decamer primers were tested. and twenty six of them yielded amplification products. Fourteen primers gave products with at least one polymorphic DNA fragment. Among a total of 119 amplified fragments 33 were polymorphic. These RAPD markers enabled the identification of asparagus cultivars. Unique markers for cultivars were: Snow-bands 475 bp, 772 bp, 412 bp, 935 bp and 820 bp amplified by primers D5, OPA-07, OPA-09, OPA-10 and OPA-18, respectively. Steline-bands 645 bp, 680 bp and 997 bp amplified by primers A32, OPA-03 and OPA-09, respectively. A band 903 bp, amplitied by primer OPA-12, is a marker for Imperial, and a band 420 bp, amplified by primer D52, is a marker for Larac. Cultivar UC-157 could be identified by a combination of shared polymorphic bands. The pairwise marker difference between cultivars ranged from 0.08 to 0.17. A phenogram of the genetic relationship based on RAPD fits with the known origin of the cultivars. 相似文献
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一串红品种（系）遗传多样性RADP分析 总被引：4，自引：1，他引：3
tianyelin@sina.com 《中国农学通报》2006,22(5):76-76
(农业应用新技术北京市重点实验室/北京农学院园林系,北京102206) 相似文献
18.
Genetic variation among five elite winter barley cultivars (H. vulgare L.) currently grown in Bulgaria was assessed at the molecular level using restriction fragment length polymorphism (RFLP)
and randomly amplified polymorphic DNA (RAPD) markers. The present study sampled RFLPs in four well characterized multigene
families in barley: the seed storage protein loci; the 18S, 5.8S and 26S ribosomal DNA loci; the loci coding for 5S ribosomal
RNA and the loci coding subunit α of ATP-A complex in the mitochondrial genome. RFLPs were detected in three out of five investigated
chromosomal loci in the barley cultivars studied. RAPD assay using arbitrary 10-base primers was applied to generate amplified
length polymorphic markers in barley. Overall a total of 15 polymorphic phenotypes were found among the studied barley cultivars
by using 11 out of 25 tested primers. All RAPDs were considered as dominant genetic markers except for two, where PCR and
Southern blot
analysis indicated the presence of codominant amplification products. Five RAPD polymorphisms in F1 and F2 progenies of the cross between Alpha and Obzor were inherited in Mendelian fashion. The determined values for the genetic
variation proved a high genetic similarity among the tested cultivars. Genetic similarity (GS) calculated from RFLP and RAPD data ranged from 0.888 to 0.997 with a mean GS – 0.933.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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The applicability of random amplified polymorphic DNA (RAPD) markers in the cultivated rubber tree, Hevea, was evaluated using 43 decamer oligonucleotide primers in a set of 24 clones selected in different South-East Asian countries. A total of 220 0.35–3.5 kb DNA fragments were amplified, of which 111 were polymorphic. Of these, 80 fragments (RAPD markers) which were repeatable and clearly scorable across all genotypes were used to estimate genetic distances among the clones tested. The estimated genetic distances ranged from 0.05 (RRII 308 and PB 5/51) to 0.75 (RRIC 100 and SCATC 88–13). A mean genetic distance of 0.5 indicates a rather high genetic variability among the tested clones. As expected, because of the breeding history of Hevea, UPGMA cluster analysis and Principal Coordinate Analysis (PCoA) indicated the absence of a distinct geographical grouping. The possible application of RAPD markers for clone identification and also for analysis of genetic relationships among Hevea clones is discussed. 相似文献