共查询到20条相似文献，搜索用时 15 毫秒
1.
N Davis S Ghosh D L Simmons P Tempst H C Liou D Baltimore H R Bose 《Science (New York, N.Y.)》1991,253(5025):1268-1271
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I kappa B: a specific inhibitor of the NF-kappa B transcription factor 总被引：273，自引：0，他引：273
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HTLV-I tax induces cellular proteins that activate the kappa B element in the IL-2 receptor alpha gene 总被引：91，自引：0，他引：91
D W Ballard E B?hnlein J W Lowenthal Y Wano B R Franza W C Greene 《Science (New York, N.Y.)》1988,241(4873):1652-1655
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Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif 总被引：67，自引：0，他引：67
L M Staudt R G Clerc H Singh J H LeBowitz P A Sharp D Baltimore 《Science (New York, N.Y.)》1988,241(4865):577-580
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A new member of the leucine zipper class of proteins that binds to the HLA DR alpha promoter 总被引：42，自引：0，他引：42
H C Liou M R Boothby P W Finn R Davidon N Nabavi N J Zeleznik-Le J P Ting L H Glimcher 《Science (New York, N.Y.)》1990,247(4950):1581-1584
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A G protein gamma subunit shares homology with ras proteins 总被引：18，自引：0，他引：18
Guanine nucleotide binding proteins (G proteins) that transduce signals from cell surface receptors to effector molecules are made up of three subunits, alpha, beta, and gamma. A complementary DNA clone that encodes a 71-amino acid protein was isolated from bovine brain; this protein contains peptide sequences that were derived from the purified gamma subunit of Gi and Go. The primary sequence of this G protein gamma subunit (G gamma) has 55 percent homology to the gamma subunit of transducin (T gamma) and also has homology to functional domains of mammalian ras proteins. The probe for isolating the clone was generated with the use of the polymerase chain reaction (PCR). The extent of divergence between T gamma and G gamma, the isolation of homologous PCR-generated fragments, and the differences between the predicted amino acid sequence of G gamma and that derived from the gamma subunit of Gi and Go indicate that gamma subunits are encoded by a family of genes. 相似文献
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Kappa B-specific DNA binding proteins: role in the regulation of human interleukin-2 gene expression 总被引：72，自引：0，他引：72
B Hoyos D W Ballard E B?hnlein M Siekevitz W C Greene 《Science (New York, N.Y.)》1989,244(4903):457-460
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Immunochemical proof that a novel rearranging gene encodes the T cell receptor delta subunit 总被引：58，自引：0，他引：58
H Band F Hochstenbach J McLean S Hata M S Krangel M B Brenner 《Science (New York, N.Y.)》1987,238(4827):682-684
The T cell receptor (TCR) delta protein is expressed as part of a heterodimer with TCR gamma, in association with the CD3 polypeptides on a subset of functional peripheral blood T lymphocytes, thymocytes, and certain leukemic T cell lines. A monoclonal antibody directed against TCR delta was produced that binds specifically to the surface of several TCR gamma delta cell lines and immunoprecipitates the TCR gamma delta as a heterodimer from Triton X-100 detergent lysates and also immunoprecipitates the TCR delta subunit alone after chain separation. A candidate human TCR delta complementary DNA clone (IDP2 O-240/38), reported in a companion paper, was isolated by the subtractive library approach from a TCR gamma delta cell line. This complementary DNA clone was used to direct the synthesis of a polypeptide that is specifically recognized by the monoclonal antibody to TCR delta. This complementary DNA clone thus corresponds to the gene that encodes the TCR delta subunit. 相似文献
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Sequence-specific binding of human Ets-1 to the T cell receptor alpha gene enhancer 总被引：48，自引：0，他引：48
I C Ho N K Bhat L R Gottschalk T Lindsten C B Thompson T S Papas J M Leiden 《Science (New York, N.Y.)》1990,250(4982):814-818
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Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase 总被引：37，自引：0，他引：37
P M Kane C T Yamashiro D F Wolczyk N Neff M Goebl T H Stevens 《Science (New York, N.Y.)》1990,250(4981):651-657
The TFP1 gene of the yeast Saccharomyces cerevisiae encodes two proteins: the 69-kilodalton (kD) catalytic subunit of the vacuolar proton-translocating adenosine triphosphatase (H(+)-ATPase) and a 50-kD protein. The 69-kD subunit is encoded by the 5' and 3' thirds of the TFP1 coding region, whereas the 50-kD protein is encoded by the central third. Evidence is presented that both the 69-kD and 50-kD proteins are obtained from a single translation product that is cleaved to release the 50-kD protein and spliced to form the 69-kD subunit. 相似文献
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Isolation of human C-reactive protein complementary DNA and localization of the gene to chromosome 1 总被引：7，自引：0，他引：7
A S Whitehead G A Bruns A F Markham H R Colten D E Woods 《Science (New York, N.Y.)》1983,221(4605):69-71
With a synthetic oligonucleotide mixture as probe, complementary DNA clones of C-reactive protein were isolated from an adult human liver complementary DNA library. The clones ranged in size from 700 to 1100 base pairs and were identified by partial DNA sequence analysis. One complementary DNA clone was used as a probe for hybridization with human-rodent DNA's isolated from somatic cell hybrids and bound to nitrocellulose filters (Southern blot analysis) to assign the human C-reactive protein gene to chromosome 1. 相似文献
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Li B Yu H Zheng W Voll R Na S Roberts AW Williams DA Davis RJ Ghosh S Flavell RA 《Science (New York, N.Y.)》2000,288(5474):2219-2222
T helper 1 (TH1) cells mediate cellular immunity, whereas TH2 cells potentiate antiparasite and humoral immunity. We used a complementary DNA subtraction method, representational display analysis, to show that the small guanosine triphosphatase Rac2 is expressed selectively in murine TH1 cells. Rac induces the interferon-gamma (IFN-gamma) promoter through cooperative activation of the nuclear factor kappa B and p38 mitogen-activated protein kinase pathways. Tetracycline-regulated transgenic mice expressing constitutively active Rac2 in T cells exhibited enhanced IFN-gamma production. Dominant-negative Rac inhibited IFN-gamma production in murine T cells. Moreover, T cells from Rac2-/- mice showed decreased IFN-gamma production under TH1 conditions in vitro. Thus, Rac2 activates TH1-specific signaling and IFN-gamma gene expression. 相似文献
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Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase 总被引：27，自引：0，他引：27
A complementary DNA clone encoding the alpha subunit of the adenylate cyclase stimulatory G protein (Gs) was isolated and identified. A bovine brain complementary DNA library was screened with an oligonucleotide probe derived from amino acid sequence common to known G proteins. The only clone that was obtained with this probe has a complementary DNA insert of approximately 1670 base pairs. An antibody to a peptide synthesized according to deduced amino acid sequence reacts specifically with the alpha subunit of Gs. In addition, RNA that hybridizes with probes made from the clone is detected in wild-type S49 cells; however, cyc- S49 cells, which are deficient in Gs alpha activity, are devoid of this messenger RNA. 相似文献
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Identification of a zinc finger protein that binds to the sterol regulatory element 总被引：36，自引：0，他引：36
T B Rajavashisth A K Taylor A Andalibi K L Svenson A J Lusis 《Science (New York, N.Y.)》1989,245(4918):640-643
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Cyclosporin A specifically inhibits function of nuclear proteins involved in T cell activation 总被引：79，自引：0，他引：79
E A Emmel C L Verweij D B Durand K M Higgins E Lacy G R Crabtree 《Science (New York, N.Y.)》1989,246(4937):1617-1620
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Chromosomal location of human T-cell receptor gene Ti beta 总被引：10，自引：0，他引：10
P E Barker F H Ruddle H D Royer O Acuto E L Reinherz 《Science (New York, N.Y.)》1984,226(4672):348-349
A complementary DNA probe corresponding to the beta-chain gene of Ti, the human T lymphocyte receptor, has been molecularly cloned. The chromosomal origin of the Ti beta gene was determined with the complementary DNA by screening a series of 12 cell hybrid (mouse X human) DNA's containing overlapping subsets of human chromosomes. DNA hybridization (Southern) experiments showed that the human Ti beta gene resides on chromosome 7 and is thus not linked to the immunoglobulin loci or to the major histocompatibility locus in humans. 相似文献