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1.
放牧是最主要的草地利用模式，直接或间接地影响草地物质循环和能量流动，放牧强度对草地的健康状况和演替方向起决定作用。本文基于40篇内蒙古草原放牧相关文献数据，通过meta分析探讨温带草原对放牧强度的响应特征。结果表明，与未放牧草地相比，轻度放牧草地对群落植物地上、地下生物量和土壤全氮和全磷含量无显著影响，而土壤有机碳、微生物生物量碳、细菌和真菌数量分别显著上升3.60%、7.80%、11.40%和10.83%（P<0.05）；中度放牧下群落植物地下生物量和土壤微生物数量无显著变化，而地上生物量和土壤有机碳、全氮、全磷和微生物生物量氮含量分别显著降低21.62%、4.44%、2.15%、8.35%和6.76%（P<0.05）；重度放牧下群落植物地上和地下生物量，土壤有机碳、全氮、全磷、微生物生物量碳含量，细菌和放线菌数量分别显著下降39.72%、16.30%、7.62%、6.46%、8.03%、8.76%、12.92%和18.27%（P<0.05）。以上结果表明轻度放牧有利于土壤肥力和草地生产力的保持和提升，而当放牧干扰超出一定的限度时，草地各项功能均显著下降而发生退化。本研究可为内蒙古温带草原的合理利用和适应性管理提供理论基础。 相似文献
2.
放牧对荒漠草原土壤养分及微生物量的影响 总被引：1，自引：1，他引：1
[目的]探讨不同放牧强度对荒漠草原植被多样性、土壤理化性状、土壤养分及土壤微生物量的影响。[方法]以围封禁牧草地为对照,采用野外调查和室内分析的方法,对不同放牧强度下的草地土壤及植被展开调查。[结果]随放牧强度的增加,荒漠草原植被盖度、物种多样性、地上生物量、土壤养分和微生物量显著降低,土壤容重和pH值呈增加趋势,土壤电导率呈先增加后降低趋势,地下生物量则没有明显变化趋势;在植被作用下土壤养分和微生物量垂直方向表现递减规律并且在表层富集,"表聚性"较为明显;在放牧干扰下土壤全磷变异系数最高;放牧并没有改变荒漠草原土壤养分和微生物量的垂直分布特征;相关分析表明,放牧干扰下土壤微生物量与土壤养分之间具有较强的相关性,二者与土壤含水量也有较强的相关性。[结论]放牧强度对土壤全磷的空间变异影响较大,并且土壤微生物量对于放牧干扰的敏感性高于土壤养分全量;土壤养分和微生物量等地下生态系统各指标之间具有统一性。 相似文献
3.
以内蒙古锡林格勒典型草原羊草群落为研究对象,以长期放牧样地为参照(CK),开展了短期休牧对典型群落土壤养分和植物群落特征的影响研究。结果表明:休牧后典型草原物种特征发生明显的变化,与对照相比,休牧后群落高度、盖度、密度、地上生物量、地下生物量和枯落物量均有所增加,并且随月份(8月、9月、10月)的变化呈递减趋势,休牧和对照样地地下生物量均随土层深度的增加而降低。不同物种多度均随着月份的增加而降低,相同月份植物物种数目均表现为休牧对照,与对照相比,禾本科草的种群密度增加。休牧后典型草原丰富度指数、均匀度指数、多样性指数均有所增加。土壤总孔隙度与土壤容重变化趋势相反,不同月份土壤容重表现为休牧对照,土壤总孔隙度则表现为休牧对照。休牧条件下,土壤养分(有机碳、全氮、全钾、速效磷、速效氮、速效钾)基本呈一致的变化规律,对照样地和休牧样地土壤养分均随着月份的增加呈递增趋势,不同月份之间土壤养分含量均表现为休牧对照,其中全磷在休牧和对照之间差异不显著(p0.05)。冗余分析(RDA)的结果显示,沿着RDA的第1排序轴,随着显著性影响因子(土壤养分各指标)的增加,丰富度指数、多样性指数、优势度指数逐渐增加,并且丰富度指数、多样性指数、优势度指数与土壤容重呈负相关,排序轴可反映土壤因子的梯度变化特征,表明利用土壤养分作为表征土壤—植被关系的敏感性土壤指示因子是可行的。综合分析表明:休牧有利于锡林郭勒典型草原植被的恢复,本研究为锡林郭勒典型草原的合理管理利用及其可持续利用提供科学依据。 相似文献
4.
不同放牧强度对锡林郭勒典型草原生产力及碳储量的影响 总被引：3，自引：1，他引：2
以典型草原羊草群落为研究目标,以1979年围封样地为参照(NG),选择轻度(LG)、中度(MG)、重度(HG)放牧样地开展了放牧强度对典型群落生产力及碳储量的研究。结果表明:随着放牧强度的增加,物种丰富度指数、均匀度指数、多样性指数、优势度指数均降低,其中LG,MG和HG均与NG达到显著差异水平(p0.05),并且均表现出NGLGMGHG变化趋势;随着放牧强度增加,地上生物量和凋落物量呈递减趋势,NG地上植被和凋落物碳储量最高,其大小依次为NGLGMGHG,放牧显著降低了群落的地上植物有机碳储量;随着土层深度的增加,根系有机碳储量呈现减少的趋势,从地下0—60cm根系有机碳储量来看,大小表现为NGLGMGHG,根系总有机碳储量在不同放牧强度间的变化主要受0—20cm根系有机碳储量的影响;土壤有机碳含量随放牧强度的增加呈先增加后减少趋势,随土层深度的增加呈降低趋势,不同放牧强度只对0—40cm土层有机碳含量影响差异显著(p0.05),在40cm以下土层,其影响不显著(p0.05),0—60cm土壤有机碳储量序列为MGLGNGHG,MG的固碳能力最强,说明放牧对植被—土壤系统有机碳储量的影响具有非同步性,轻度和中度放牧有助于生态系统有机碳储量的积累,而重度放牧草原生态系统有机碳储量显著降低。土壤碳贮量占总碳贮量的80%以上,是草原碳储量的主体部分,各生态系统有机碳储量比例均表现为土壤根系地上植物凋落物;植被地上碳储量与0—20cm土壤碳储量存在着显著的相关性(p0.05),回归分析结果表明土壤有机碳储量与土层深度具有较好的相关关系。 相似文献
5.
土壤活性有机碳是土壤中较为活跃的化学组分,能反映土壤有机碳(SOC)的有效性,可作为土壤质量和肥力的指示性指标。研究探讨放牧干扰对羊草草甸草原土壤活性有机碳的影响,从而了解放牧对草地生态系统中碳循环的影响机制,旨在为退化草地生态恢复过程提供理论支撑。依托于呼伦贝尔草原生态系统国家野外科学观测研究站的长期肉牛放牧平台,分析6种不同放牧强度[不放牧(G0.00)、较轻度放牧(G0.23)、轻度放牧(G0.34)、中度放牧(G0.46)、重度放牧(G0.69)、极重度放牧(G0.92)]下羊草草甸草原土壤活性有机碳的变化,并探讨其相关性。结果表明:(1)土壤有机碳和活性碳组分的含量在不同土层间差异显著,随着土层深度的增加,SOC和活性有机碳[颗粒有机碳(POC)、可溶性有机碳(DOC)、微生物生物量碳(MBC)、易氧化有机碳(EOC)]含量均显著降低。(2)不同土层活性有机碳占比与放牧强度呈线性正相关,随着放牧强度的增加土壤活性有机碳占比也随之增加,不同放牧强度不同土层下土壤各形态活性有机碳占比呈POC>EOC>MBC>DOC的趋势,且EOC、MBC和POC占比随土壤深度增加而降低。(3)随着放牧强度的增加,植被群落地上生物量、地下生物量和土壤水分均呈下降趋势。(4)放牧强度与SOC和DOC呈显著负相关;植被群落地上生物量与土壤碳组分间呈显著正相关;地下生物量与DOC之间呈极显著正相关;土壤温度与POC呈显著负相关;土壤水分与POC呈极显著正相关,与SOC之间呈显著正相关。研究表明:放牧会降低地上生物量、地下生物量和土壤水分,适度放牧不仅能增加土壤有机碳和活性碳组分,而且能增加土壤活性碳占比,且符合中度干扰 相似文献
6.
以天山中部中科院巴音布鲁克草原生态观测站三种类型草地长期（26 a）围栏封育样地为研究对象,通过野外调查取样结合室内分析的方法,研究了长期（26 a）围栏封育对草地土壤有机碳和微生物量碳含量的影响,结果表明：（1）围栏外（自然放牧条件下）,表层的土壤有机碳含量为高寒草甸（165.29 g·kg-1）〉高寒草甸草原（98.73 g·kg-1）〉高寒草原（83.54 g·kg-1）,微生物量碳含量依次为高寒草甸草原（181.70 mg·kg-1）〉高寒草甸（146.37 mg·kg-1）〉高寒草原（43.06 mg·kg-1）。围栏封育后,高寒草甸、高寒草甸草原、高寒草原表层土壤有机碳含量分别提高了11.37%、3.26%和2.21%;高寒草甸草原和高寒草甸微生物量碳含量分别增长2.89%和12.04%,而高寒草原降低40.36%。（2）从围栏内外土壤剖面来看,土壤有机碳、微生物量碳含量随着土壤深度的增加依次降低,微生物熵也随土壤深度的增加呈现降低的趋势。（3）微生物量碳含量与土壤速效钾、全磷含量达到极显著负相关（P〈0.01）,与速效磷含量达到极显著正相关（P〈0.01）,与土壤有机碳、全氮、全钾含量呈显著正相关（P〈0.05）与土壤速效氮含量正相关,但不显著。 相似文献
7.
荒漠草原土壤酶与土壤养分的动态研究 总被引：1，自引：0，他引：1
研究了内蒙古荒漠草原不同利用强度单元内土壤养分以及5种酶活性,并对土壤酶活性与土壤养分含量之间的相关性进行了分析。结果表明,草原的利用强度直接影响着草地的土壤养分状况和酶活性,轻度利用有利于土壤中5种酶活性的增加,重度利用则导致土壤转化酶和蛋白酶活性显著降低。轻度利用增加了土壤中有机质、全氮和全磷的含量,重度利用则导致土壤有机质、全磷、全氮和全钾含量降低。脲酶和多酚氧化酶的活性在年季内变化幅度较大。相关性分析表明,蛋白酶与各养分之间的相关性最大。 相似文献
8.
采用室外取样与室内分析结合的方法对祁连山高寒杜鹃灌丛草地不同放牧干扰条件下土壤、根系养分化学计量比进行研究。结果表明:1)植物群落地下生物量随土层深度增加而迅速减少,60%～70%根系聚集于0～10 cm土层,随放牧压力增加活根向土壤深层转移。2)总根系生物量及其C、N、P储量随牧压增加均下降,重牧与轻牧相比C、N、P养分储量分别下降26%、17%、27%;表层单位质量活根C含量轻牧最高、N含量中度放牧最高、P含量重牧最高;死根N、P变化与活根相反。3)随牧压增加土壤有机碳、全氮密度以及N、P速效养分均上升,全磷含量相对稳定,但表层全磷含量下降。数据分析得出,休牧增加了根系生物量的同时,表层土壤根系的C/N、C/P比值升高,N/P比值降低。说明根系对土壤C积累及养分循环起重要作用,而生长季休牧有利于高寒灌丛草地土壤养分保持,这与高寒地区植物生长缓慢特性相适应。 相似文献
9.
[目的] 研究不同放牧强度对荒漠草原植被特征、土壤物理性能及其土壤侵蚀的影响,为荒漠草原水土流失治理和退化及生态系统修复提供科学依据。[方法] 以内蒙古希拉穆仁荒漠草原为研究区,采用原位监测和模拟试验相结合的方法,对重度、中度、轻度和无放牧4种不同放牧强度样地的植被群落、土壤物理性质、土壤侵蚀过程开展了研究。[结果] ①随着放牧强度的增加,群落平均盖度、高度和地上生物量呈降低趋势,物种丰富度指数、多样性指数及均匀度指数整体呈上升趋势。②土壤砂粒含量随放牧强度的增加呈上升趋势,表层土壤(0—10 cm)容重及入渗性能更易受放牧影响。③相同降雨强度下,随放牧强度的增加,土壤的侵蚀产沙量随之增加。土壤初渗速率、Simpson优势度指数和土壤砂粒含量是影响不同放牧强度下土壤侵蚀特征的关键因素。[结论] 放牧显著影响荒漠草原植被群落特征及土壤水文物理性能,因此,建议该区放牧强度控制在中度(2羊单位/hm2)及以下,对于受重度放牧破坏的草原区,围栏封育的同时还可适当进行人工补播或利用微生物改良土壤来提升土壤抗蚀性。 相似文献
10.
[目的]明晰各植物群落土壤有机碳含量，以期为草原保护提供理论依据。[方法]以希拉穆仁荒漠草原围封区6种植物群落(短花针茅、洽草、羊草、克氏针茅、银灰旋花、蒙古韭)为研究对象，对0—30 cm土层各植物群落土壤机械组成、土壤有机碳含量和根系特征进行研究。[结果](1) 6种植物群落0—30 cm土层土壤机械组成主要以粉粒和极细砂为主，其平均值分别介于26.50%～54.62%和16.90%～34.08%。(2) 6种植物群落根系生物量随土层深度逐渐减小，且根系生物量总量介于229.21～731.71 g/m2。由根系分布特征判断，短花针茅群落各土层根系呈“E”型分布，其他植物群落均呈“V”型分布。(3)各植物群落土壤有机碳含量随土层深度逐渐降低。0—30 cm平均有机碳含量介于6.07～11.75 g/kg。(4)根系生物量、土壤有机碳含量与黏粒含量呈极显著正相关，与粉粒含量呈显著正相关，表明土壤粉粒、黏粒含量越多，植物群落根系生物量和土壤有机碳越丰富。[结论]围封可以有效促使土壤有机碳增加，但有机碳对不同植被群落的响应有所差异，草原有机碳储量准确度应根据草原植被群... 相似文献
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《国际水土保持研究(英文)》2023,11(3):549-560
In arid and semi-arid desert steppe areas, grazing exclusion with fencing is widely regarded as an effective strategy for restoring degraded vegetation and enhancing the quality of degraded soil. In this study, we hypothesized that grazing exclusion caused by fencing enhances both vegetation and soil properties, and that the longer an area is fenced, the more considerable the improvement. We conducted an observational study wherein random sampling was utilized to select 9 plots fenced for ten or more years, 25 plots fenced for four to nine years, 25 plots fenced for one to three years and 29 free-grazing plots within an area of approximately 63,000 km2 of Inner Mongolia desert steppe. A one-way ANOVA revealed no significant differences in the characteristics of grassland vegetation or soil properties between grasslands fenced for one to three years and free-grazing grassland. After 4 years of fencing, noticeable increases in above-ground biomass, litter content, Simpson index, soil organic carbon, and available nitrogen were observed. Significant positive differences in vegetation coverage, height, species richness, soil available phosphorus, and available potassium were associated with plots with a minimum of 10 years of fencing. The soil layer with the greatest difference in the fenced-in areas for soil organic carbon was at 0–25 cm. For available nitrogen and available phosphorus, fencing produced the most significant differences in the 0–20 cm soil layer, while for available potassium, fencing produced the most significant differences in the 0–30 cm soil layer. However, the fencing did not indicate any statistically significant differences in terms of clay, silt, and sand content in any soil layer. The data support our hypothesis that grazing exclusion improves both vegetation and soil properties, and that longer periods of grazing exclusion result in greater degrees of improvement. This research offers technical guidance for the reasonable choice of fencing time across a vast area of the Inner Mongolian desert steppe. 相似文献
12.
青藏高原三江源地区的高寒草甸面临着严峻的退化问题，人工建植是三江源地区退化草地的重要修复方式。为探究地下生物对草地人工恢复措施的响应，本研究比较了三江源地区高寒草甸不同恢复期人工草地(建植1、5和10 a)的土壤线虫群落变化。结果显示：与原生植被样地相比，所有恢复期样地的植物地下生物量降低，土壤容重、pH、全磷、全钾和硝态氮含量升高，表明人工草地系统的初级生产力和土壤特性尚未恢复到原生草地状态。不同恢复期样地中土壤线虫的均匀度和多样性指数均显著高于原生植被样地。此外，不同恢复期样地的线虫多度、代谢足迹以及成熟度指数均随恢复年限的增加而增加。相关性分析结果表明，土壤线虫多度与植物地下生物量、土壤有机质、全氮、全磷、矿质氮和速效氮含量显著正相关(P<0.05)，与土壤pH、全钾和容重显著负相关(P<0.05)。尽管三江源区退化草地的人工恢复措施尚未完全恢复牧草生产力至原生植被状态，但土壤生物结构及功能具有改善的趋势，显示该地区退化草地生态系统具有较强的恢复潜力。 相似文献
13.
Livestock grazing is one of the most important factors influencing the soil nutrients, soil surface, vegetation diversity, and the above-ground biomass production in a natural rangeland ecosystem. An experiment was conducted to evaluate the influence of the long-term grazing on soil pH, electrical conductivity (EC), total organic carbon (TOC), total nitrogen (TN), available phosphorus (Available P), exchangeable potassium (Exchangeable K), diversity and the above-ground biomass production. This study was conducted in Maraveh Tapeh hilly rangelands of Golestan province, Iran. Soil and vegetation samplings were conducted based on the randomized systematic method. Three 150 m transects were positioned randomly within each grazing management practice. Soil samples were systematically taken every 3 m along each transect. Thirty (30) randomly chosen quadrats were sampled in each transect for vegetation diversity and biomass assessment. The effects of grazing management practices and aspect on soil nutrients, vegetation diversity, and biomass production were detected by analysis of variance (ANOVA) (at α = 0.05). Results of this study indicated, Exclosure north aspects had the maximum soil pH. There were significant differences found between soil electrical conductivity values in the grazed south aspect and the other treatments. Soil TOC, TN, available P and exchangeable K values increased significantly in the exclosure north aspects. Shannon diversity index and the above-ground biomass production had highest values in the exclosure north aspects. 相似文献
14.
Markus Steffens Angelika Kölbl Marcus Giese Carsten Hoffmann Kai Uwe Totsche Lutz Breuer Ingrid Kögel‐Knabner 《植物养料与土壤学杂志》2009,172(1):78-90
It is not clear from the literature how the spatial distribution of topsoil and vegetation properties is affected by grazing cessation. Thus, the objective of this study was to elucidate if long‐term grazing exclosure increases spatial heterogeneity of topsoil and vegetation properties in a steppe ecosystem in NE China. Variograms and crossvariograms were calculated for bulk density, organic carbon (OC), total N, and total S concentration, δ13C, pH, Ah horizon thickness, vegetation cover, and aboveground biomass. Five sites with different grazing intensities (ungrazed since 1979, ungrazed since 1999, winter grazing, continuously grazed, heavily grazed) were sampled with two different grid sizes, allowing the exploration of scale effects. Small grids (15 m spacing, 5 m nested sampling) exhibited a different spatial structure compared to large grids (50 m spacing, 10 m nested sampling). Spatial distribution in small grids changed with grazing intensity. Generally, heterogeneity of topsoil properties increased with decreasing grazing intensity from a homogeneous to a patchy distribution. We attribute this to vegetation recovery/succession and deposition of windblown material in ungrazed areas. The plot ungrazed since 1999 showed different spatial dependencies than continuously and heavily grazed plots, but has not yet reached the high variability of the plot which was ungrazed since 1979. Large grid sampling did not detect small‐scale variability or grazing impacts, but showed spatial dependencies that were attributed to topography or soil erosion/deposition. Low OC concentration and low Ah thickness were associated with hilltop and shoulder positions, resulting in lower OC stocks at these topographic units. 相似文献
15.
M. Wiesmeier M. Steffens C. W. Mueller A. Kölbl A. Reszkowska S. Peth R. Horn I. Kögel‐Knabner 《European Journal of Soil Science》2012,63(1):22-31
Spatial inaccessibility of soil organic carbon (SOC) for microbial decay within soil aggregates is an important stabilization mechanism. However, little is known about the stability of aggregates in semiarid grasslands and their sensitivity to intensive grazing. In this study, a combined approach using soil chemical and physical analytical methods was applied to investigate the effect of grazing and grazing exclusion on the amount and stability of soil aggregates and the associated physical protection of SOC. Topsoils from continuously grazed (CG) and ungrazed sites where grazing was excluded from 1979 onwards (UG79) were sampled for two steppe types in Inner Mongolia, northern China. All samples were analysed for basic soil properties and separated into free and aggregate‐occluded light fractions (fLF, oLF) and mineral‐associated fractions. Tensile strength of soil aggregates was measured by crushing tests. Undisturbed as well as artificially compacted samples, where aggregates were destroyed mechanically by compression, were incubated and the mineralization of SOC was measured. For undisturbed samples, the cumulative release of CO2‐C was greater for CG compared with UG79 for both steppe types. A considerably greater amount of oLF was found in UG79 than in CG soils, but the stabilities of 10–20‐mm aggregates were less for ungrazed sites. Compacted samples showed only a slightly larger carbon release with CG but a considerably enhanced mineralization with UG79. We assume that the continuous trampling of grazing animals together with a smaller input of organic matter leads to the formation of mechanically compacted stable ‘clods’, which do not provide an effective physical protection for SOC in the grazed plots. In UG79 sites, a greater input of organic matter acting as binding agents in combination with an exclusion of animal trampling enhances the formation of soil aggregates. Thus, grazing exclusion promotes the physical protection of SOC by increasing soil aggregation and is hence a management option to enhance the C sequestration potential of degraded steppe soils. 相似文献
16.
Luis Lopez-Sangil Johannes Rousk Håkan Wallander Pere Casals 《Biology and Fertility of Soils》2011,47(2):129-138
The present study investigated the effects of land-use abandonment on the soil decomposer community of two grazed Mediterranean
ecosystems (an annual grassland with scattered holm oaks and a low-density shrubland). To test the influence of grazing abandonment,
a set of plots within each site were fenced and kept undisturbed during 4–5 years, during which above-ground plant community
structure was monitored. After that, soil samples were collected from grazed and abandoned plots corresponding to the three
different soil conditions: away from (“grass”) and below tree canopies (“oak”) within the annual grassland, and from the shrubland
(“shrub”). Soil samples were split into two different layers (0–5 and 5–15 cm) and then analyzed for saprotrophic fungal (acetate
into ergosterol incorporation) and bacterial (leucine incorporation) growth rates. Ergosterol content (as a fungal biomass
estimator) and a standard set of soil chemistry variables were also measured. After 5 years of grazing exclusion, saprotrophic
fungal growth rate clearly increased in both grass and oak surface layers whereas bacterial growth rate was not altered. This
translated into significantly higher fungal-to-bacterial (F/B) growth rate ratios within the ungrazed plots. Similar trends
were observed for the shrub soils after 4 years of exclusion. On the contrary, abandonment of grazing had negligible effects
on the ergosterol content, as well as on the soil chemical variables (soil organic carbon, total N, C/N ratio, and pH), in
all the three soil conditions assessed. These results indicated a shift toward a more fungal-dominated decomposer activity
in soils following cessation of grazing and highlighted the sensitivity of the microbial growth rate parameters to changes
associated with land use. Moreover, there were evidences of a faster fungal biomass turnover in the ungrazed plots, which
would reflect an accelerated, though not bigger, fungal channel in soil organic matter mineralization. 相似文献
17.
以内蒙古贝加尔针茅草原、大针茅草原和克氏针茅草原为研究对象,采用氯仿熏蒸法和磷脂脂肪酸（PLFA）分析方法研究了放牧与围栏条件下内蒙古针茅草原土壤微生物生物量和群落结构特征的变化情况。研究表明放牧与围栏草地土壤微生物生物量和群落结构差异显著。氯仿熏蒸法分析结果表明内蒙古针茅草原土壤微生物生物量碳的含量介于166.6-703.5mg·kg^-1之间,微生物生物量氮含量介于30.34-92.15mg·kg^-1之间,其中贝加尔针茅草原土壤微生物生物量碳、氮最高,大针茅草原次之,克氏针茅草原则最低。放牧条件下,贝加尔针茅草原、大针茅草原土壤微生物生物量碳、氮显著低于围栏草地,克氏针茅草原则无显著变化。PLFA分析结果显示,内蒙古针茅草原土壤微生物群落PLFAs种类、含量丰富,共检测出28种PLFA生物标记磷脂脂肪酸,并且以直链饱和脂肪酸和支链饱和脂肪酸为主,相对含量占总量的2/3左右,其中贝加尔针茅草原土壤微生物含量最丰富,其围栏样地土壤的PLFA含量达到27.3nmol·g-1,大针茅草原和克氏针茅草原依次降低。围栏条件下,各类型草原土壤细菌脂肪酸与总PLFA含量均显著高于放牧草地,真菌脂肪酸含量则因草原类型不同各有差异;放牧导致各类型草原革兰氏阳性细菌PLFAs/革兰氏阴性细菌PLFAs（GPPLFAs/GNPLFAs）比值显著降低,而除了克氏针茅草原,细菌PLFAs/真菌PLFAs比值则显著升高。PLFAs主成分分析表明,放牧和围栏处理对内蒙古针茅草原土壤微生物群落结构产生影响,且围栏处理的影响程度大于放牧处理。经相关分析表明,氯仿熏蒸法和PLFA分析方法之间有很好的一致性,且土壤微生物PLFAs与土壤有机质、全磷、硝态氮显著相关。 相似文献