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1.
Two laboratory experiments were used to investigate the effect of the earthworm Lumbricus terrestris on transport of genetically marked Pseudomonas fluorescens inocula through soil microcosms. The microcosms comprised cylindrical cores of repacked soil with or without earthworms. Late log-phase cells of P. fluorescens, chromosomally marked with lux genes encoding bioluminescence, were applied to the surface of soil cores as inoculated filter paper discs. In one experiment, 5 and 10 days after inoculation, cores were destructively harvested to determine concentrations of marked pseudomonads with depth relative to the initial inoculum applied. Transport of the bacteria occurred only in the presence of earthworms. In a second experiment cores were subjected to simulated rainfall events 18 h after inoculation with lux-marked bacteria at 3-day intervals over a 24-day period. Resulting leachates were analysed for the appearance of the marked bacteria, and after 28 days cores were destructively harvested. Although some marked cells (less than 0.1% of the inoculum applied) were leached through soil in percolating water, particularly in the presence of earthworms, the most important effect of earthworms on cell transport was through burial of inoculated litter rather than an increase in bypass flow due to earthworm channels.  相似文献   

2.
Summary The effects of soil temperature and bulk soil pH on the vertical translocation of a genetically modified Pseudomonas fluorescens inoculum were studied in reconstituted soil microcosms, in the presence and absence of growing Lolium perenne roots. The inoculated microcosms received one rainfall event per day (5 mm h-1 for 6 h) for 5 days and the resulting leachate was quantitatively assayed for the presence of the modified pseudomonad. Soil temperature affected the total number of modified pseudomonads detected in the leachate over the 5 days, with significantly lower numbers detected at 25°C compared to 5°C. The bulk soil pH also affected leaching of the inoculum, with significantly greater numbers detected in the effluent at pH 7.5 than at pH 4.5. In the absence of L. perenne, greater numbers of the modified pseudomonads were detected in the pH 7.5 soil after 5 days of leaching compared to soil at pH 4.5. L. perenne roots decreased the number of cells of the inoculum that were leached and detected in the soil after 5 days of leaching. In the soil microcosms used for the pH study the distribution of the inoculum remaining with the soil was altered by L. perenne roots. At each pH value the proportion of cells detected within the soil below the surface 2 cm of the microcosms was greater in the presence of L. perenne roots. The results of this study indicate that soil temperature, bulk soil pH, and the presence of root systems are important factors in determining the extent of inoculum translocation, and should be considered in the design and interpretation of field experiments.  相似文献   

3.
In a greenhouse pot study, we examined the availability of N to grain sorghum from organic and inorganic N sources. The treatments were15N-labeled clover residues, wheat residues, and fertilizer placed on a sandy clay loam and loamy sand soil surface for an 8-week period. Soil aggregates formed under each soil texture were measured after 8 weeks for each treatment. Significantly greater 15N was taken up and recovered by grain sorghum in sandy clay loam pots compared with loamy sand pots. Greater 15N recovery was consistently observed with the inorganic source than the organic sources regardless of soil texture or time. Microbial biomass C and N were significantly greater for sandy clay loam soil compared with the loamy sand. Microbial biomass 15N was also significantly greater in the sandy clay loam treatment compared to the loamy sand. The fertilizer treatment initially had the greatest pool of microbial biomass 15N but decreased with time. The crop residue treatments generally had less microbial biomass 15N with time. The crop residues and soil texture had a significant effect on the water-stable aggregates formed after 8 weeks of treatments. Significantly greater water-stable aggregates were formed in the sandy clay loam than the loamy sand. Approximately 20% greater water-stable aggregates were formed under the crop residue treatments compared to the fertilizer only treatment. Soil texture seemed to be one of the most important factors affecting the availability of N from organic or inorganic N sources in these soils.Contribution from the MissouriAgricultural Experiment Station, Journal Series No.12131  相似文献   

4.
Changes to soil nutrient availability and increases for crop yield and soil organic C (SOC) concentration on biochar‐amended soil under temperate climate conditions have only been reported in a few publications. The objective of this work was to determine if biochar application rates up to 20 Mg ha?1 affect nutrient availability in soil, SOC stocks and yield of corn (Zea mays L.), soybean (Glycine max L.), and switchgrass (Panicum virgatum L.) on two coarse‐textured soils (loamy sand, sandy clay loam) in S Quebec, Canada. Data were collected from field experiments for a 3‐y period following application of pine wood biochar at rates of 0, 10, and 20 Mg ha?1. For corn plots, at harvest 3 y after biochar application, 20 Mg biochar ha?1 resulted in 41.2% lower soil NH on the loamy sand; the same effect was not present on the sandy clay loam soil. On the loamy sand, 20 Mg biochar ha?1 increased corn yields by 14.2% compared to the control 3 y after application; the same effect was not present on the sandy clay loam soil. Biochar did not alter yield or nutrient availability in soil on soybean or switchgrass plots on either soil type. After 3 y, SOC concentration was 83 and 258% greater after 10 and 20 Mg ha?1 biochar applications, respectively, than the control in sandy clay loam soil under switchgrass production. The same effect was not present on the sandy clay loam soil. A 67% higher SOC concentration was noted with biochar application at 20 Mg ha?1 to sandy clay loam soil under corn.  相似文献   

5.
Two field experiments in which straw has been removed or incorporated for 17 yr (loamy sand) and 10 yr (sandy clay loam) were sampled to examine the effect of straw on the C and N contents in whole soil samples, macro-aggregate fractions and primary particle-size separates. The particle size composition of the aggregate fractions was determined. Aggregates were isolated by dry sieving. Straw incorporation increased the number of 1–20 mm aggregates in the loamy sand but no effect was noted in the sandy clay loam. Straw had no effect on the particle size composition of the various aggregate fractions. After correction for loose sand that accumulated in the aggregate fractions during dry sieving, macro-aggregates appeared to be enriched in clay and silt compared with whole soil samples. Because of the possible detachment of sand particles from the exterior surface of aggregates during sieving operations, it was inferred that the particle size composition of macro-aggregates is similar to that of the bulk soil. The organic matter contents of the aggregate fractions were closely correlated with their clay + silt contents. Differences in the organic matter content of clay isolated from whole soil samples and aggregate fractions were generally small. This was also true for the silt-size separates. In both soils, straw incorporation increased the organic matter content of nearly all clay and silt separates; for silt this was generally twice that observed for clay. The amounts of soil C, derived from straw, left in the loamy sand and sandy clay loam at the time of sampling were 4.4 and 4.5 t ha?1, corresponding to 12 and 21% of the straw C added. The C/N ratios of the straw-derived soil organic matter were 11 and 12 for the loamy sand and sandy clay loam, respectively.  相似文献   

6.
Annual potassium (K) balances have been calculated over a 40‐year period for five field experiments located on varying parent materials (from loamy sand to clay) in south and central Sweden. Each experiment consisted of a number of K fertilizer regimes and was divided into two crop rotations, mixed arable/livestock (I) and arable only (II). Annual calculations were based on data for K inputs through manure and fertilizer, and outputs in crop removal. Plots receiving no K fertilizer showed negative K balances which ranged from 30 to 65 kg ha?1 year?1 in rotation I, compared with 10–26 kg ha?1 year?1 for rotation II. On sandy loam and clay soils, the K yield of nil K plots (rotation I) increased significantly with time during the experimental period indicating increasing release of K from soil minerals, uptake from deeper soil horizons and/or depletion of exchangeable soil K (Kex). Significant depletion of Kex in the topsoil was only found in the loamy sand indicating a K supply from internal sources in the sandy loam and clay soils. On silty clay and clay soils, a grass/clover ley K concentration of ～2% (dry weight) was maintained during the 40‐year study period on the nil K plots, but on the sandy loam, loam and loamy sand, herbage concentrations were generally less than 2% K.  相似文献   

7.
The potential for transfer of the protozoan pathogen Cryptosporidium parvum through soil to land drains and, subsequently, water courses following the application of livestock waste to land was monitored in the laboratory using simulated rainfall and intact soil cores. Following irrigation over a 21-day period, Cryptosporidium parvum oocysts applied to the surface of soil cores (initial inoculum concentration 1×108 oocysts core–1) were detected, albeit in low numbers, in the leachates from clay loam and silty loam soils but not in that from a loamy sand soil. Variations in leaching patterns were recorded between replicate cores. At the end of the study soil cores were destructively sampled to establish the location of oocysts remaining within the soil. Distribution within cores was similar in all three soil types. The majority (72.8+-5.2%) of oocysts were found in the top 2 cm of soil, with numbers decreasing with increasing depth to 13.2±2.8%, 8.39±1.4%, and 5.36±1.4% at depths of 10, 20, and 30 cm, respectively.  相似文献   

8.
Summary The survival of Pseudomonas fluorescens cells encapsulated in alginate beads and colonization of wheat roots was studied in soil microcosms inoculated with the cells in alginate beads of varying composition. Cells encapsulated in beads and introduced into a non-sterile loamy sand survived better than cells added directly to the same soil. A recovery/growth step for the bead-encapsulated cells was added before they were introduced into the soil, in an attempt to obtain optimal population levels in the soil. Further, bacterial populations that grew to the highest density in the beads subsequently showed the highest survival levels in soil. The addition of 3% skim milk, or 3% skim milk and 3% bentonite clay to all bead types consistently resulted in the highest survival of the encapsulated cells in soil. Root colonization by P. fluorescens was generally not impaired by the encapsulation in alginate. One week after inoculation into the soil, encapsulated cells in the various bead types were able to colonize the wheat rhizoplane at high population levels, similar to or exceeding those found when free cells were inoculated. In a second root colonization experiment the wheat rhizoplane was also efficiently colonized 7 weeks after the inoculant cells had been introduced into the soil in different bead types. In both assays, the cells encapsulated in beads amended with skim milk plus bentonite clay showed the highest root colonization rates. It is clear, therefore, that alginate-mediated establishment of inoculants can improve inoculant effectiveness.  相似文献   

9.
Soil tilth has been defined in terms of a ‘Physical Index’ based on the product of the ratings of eight physical properties — soil depth, bulk density, available water storage capacity, cumulative infiltration or apparent hydraulic conductivity, aggregation or organic matter, non-capillary pore space, water table depth and slope. The Physical Index and a tillage guide were used to identify the tillage requirements of different soils varying in texture from loamy sand to clay in the semi-arid tropics. The physical index was 0.389 for a loamy sand, 0.518 for a black clay loam and 0.540 for a red sandy loam soil and the cumulative rating indices in summer and winter seasons were 45 and 44 for loamy sand, 52 and 51 for red sandy loam and 54 and 52 for black clay loam soils, respectively. The compaction of the loamy sand by eight passes of a 490 kg tractor-driven roller (0.75 m diameter and 1.00 m length) increased the physical index to 0.658 and chiselling of the red sandy loam and black clay loam increased the physical indices to 0.686 and 0.729, respectively. The grain yields of rainfed pearl millet and guar and irrigated pearl millet, wheat and barley increased significantly over the control (no compaction) yields by compaction.

The chiselling of the soils varying in texture from loamy sand to clay at 50 to 120-cm intervals up to 30–40 cm depth, depending upon the row spacing of seedlines and depth of the high mechanical impedance layer, increased the grain yields of rainfed and irrigated maize on alluvial loamy sand, rainfed maize on alluvial sandy loam and red sandy loam, rainfed sorghum on red sandy loam and black clay loam, irrigated sorghum on black clay loam and rainfed black gram on red sandy loam, pod yield of rainfed groundnut, tuber yield of irrigated tapioca and fresh fruit yield of rainfed tomato on red sandy loam and sugarcane yield on black clay soil, significantly over the yields of no-chiselling systems of tillage such as disc harrow and country plough.  相似文献   


10.
Abstract

Nitrate nitrogen (NO3‐N), which is an essential source of nitrogen (N) for plant growth, is now also considered a potential pollutant by the Environmental Protection Agency (EPA). This is because excess applied amounts of NO3‐N can move into streams by run‐off and into ground water by leaching, thereby becoming an environmental hazard. Soils have varied retentive properties depending on their texture, organic matter content, and cation exchange capacity (CEC). The purpose of this study was to determine the effect of soil texture on NO3‐N retention to reduce NO3‐N contamination in the environment. A sand, 85:15 sand:peat Greensmix, a loamy sand, and sandy clay loam soils were placed in 2×3 inch metal cylinders and soaked in a 240 ppm solution of NO3‐N for seven days to saturate the soil with NO3 ions. The columns were leached with water to collect 10 soil percolate samples of 50 mL each until a total volume of 500 mL was collected. Nitrate‐N was measured in each 50‐mL aliquot by automated colorimetry. The results showed that soil texture affected the retention of N03‐N in the sand, which adsorbed the least amount of NO3‐N at 119 ppm, followed by the Greensmix at 125 ppm, loamy sand at 149 ppm, and sandy clay loam at 173 ppm. More NO3‐N was released in the first 50 mL of the sand percolate at 63% followed by the Greensmix, loamy sand, and sandy clay loam at 58,46, and 37% NO3‐N released, respectively. Soils with more silt, clay, and organic matter retained more NO3‐N than the straight sand. Therefore, a straight sand would be the poorest of soil types since NO3‐N retention was low.  相似文献   

11.
土壤紧实度对伴矿景天生长及镉锌吸收性的影响研究   总被引：2，自引：2，他引：0  
王丽丽  周通  李柱  周嘉文  吴胜春  吴龙华 《土壤》2017,49(5):951-957
采集黏土、壤黏土和砂质壤土,分别设置无压实、低紧实度及高紧实度3种处理,通过盆栽试验研究了土壤紧实度对Cd、Zn超积累植物伴矿景天生长和Cd、Zn吸收性的影响。结果表明,与无压实处理比较,砂质壤土、壤黏土和黏土中伴矿景天地上部生物量在低紧实度下显著下降66.8%~83.5%、59.9%~60.4%和57.9%~71.4%;高紧实度处理却显著提高了伴矿景天的根系活力(142%~241%)。高紧实度处理显著降低了壤黏土上伴矿景天地上部Cd和Zn含量,但低紧实度对砂质壤土和黏土上伴矿景天地上部Cd和Zn含量无显著影响。与无压实处理比较,低紧实度显著降低了砂质壤土、壤黏土和黏土上伴矿景天的Cd吸取量,分别下降50.4%~73.8%、61.4%~74.9%和43.4%~63.3%,Zn吸取量下降48.7%~79.5%、73.6%~79.0%和46.1%~63.5%;土壤紧实度对壤黏土上伴矿景天的镉锌吸取效率影响最明显。  相似文献   

12.
In the present investigation, different strains of Plant growth-promoting rhizobacteria (PGPR), namely Bacillus megaterium, Pseudomonas fluorescens and Bacillus subtilis were evaluated for their growth-promoting effects on wheat as well as on soil properties under field conditions at two different sites having sandy loam and silt loam type of soils. PGPR strains were evaluated either singly or in consortia. Amongst all the treatments, wheat inoculated with consortia was found most effective as it increased grain yield up to 53% over control in silt loam soil, whereas, corresponding effects in sandy loam soil were less pronounced as an increase of 31% was observed in corresponding treatments, respectively. Enhanced effects on soil properties were also more intense in silt loam as there was an increase of 205% organic matter as against sandy loam soil where this value was 110%.  相似文献   

13.
Data transformations between soil texture schemes   总被引：1，自引：0，他引：1  
Various soil texture schemes are in current use. These differ in the size ranges of their particle fractions. There is a need to establish simple methods to correlate these conventional schemes. Therefore I have defined closed-form exponential and power law functions to fit models to cumulative particle-size distribution data. I have tested the functions for their suitability (i) to represent cumulative particle-size distribution curves and (ii) to transfer data between distributions that differ in the size ranges of the particle fractions. I found that closed-form exponential functions adequately represent the cumulative particle-size distributions of fine-textured soils (clay, silty clay, silty clay loam, clay loam, silt loam and loam texture), whilst closed-form power functions better describe the cumulative particle-size distributions of coarse-textured soils (sand, loamy sand, sandy loam, sandy clay and sandy clay loam texture). The functions defined are found to be suitable to transfer data between different texture schemes. The use of this approach is illustrated by examples of data transformations between three widely used soil texture schemes: ISSS, Katschinski's and USDA.  相似文献   

14.
 In long-term field experiments on sandy loam and loamy sand soils, the influence of conservation and conventional tillage on soil and rhizosphere microorganisms was studied. Conservation tillage stimulated rhizosphere bacteria on winter wheat, winter barley, winter rye and maize in different soil layers. Particularly the populations of Agrobacterium spp. and Pseudomonas spp. were increased. On the sandy loam, N2 fixation and nodulation of pea plants were significantly increased. No influence of different soil tillage was determined on the colonization of the rhizosphere by mycorrhiza and saprophytic fungi. Stubble residues infected with Gaeumanomyces graminis were infectious for a longer time on the soil surface than after incorporation into the soil. Received: 10 March 1998  相似文献   

15.
Summary Vertical translocation of the introduced transposon Tn5-tagged Pseudomonas fluorescens cells was studies after irrigation of 50-cm long soil columns of loamy sand. The soil in the columns was slowly brought to saturation using groundwater, and enough water was then slowly added to permit collection of the percolated water. Introduced bacteria were transported to lower soil layers to a significantly higher degree in undisturbed soil cores than in repacked cores; water transport was hampered in both core types due to high soil bulk densities. Soil bulk density affected the degree of transport of the introduced cells; progressively more cells were translocated to deeper soil layers and into the percolation water at decreasing soil bulk densities. Repeated percolation of soil at a bulk density of 1.25 caused an increase in Tn5-tagged cell numbers in the lower soil layers and in the percolated water. Further, cells initially introduced into a dry (5.3% moisture) soil were translocated to a lesser extent than cells introduced into a wetter (13% moisture) soil. Finally, wheat roots enhanced the water-induced transport of introduced cells to the 40- and 50-cm deep soil layers and into the effluent, but not to the remaining soil layers. Large soil columns such as those used in the present study are useful in assessing the transport and survival of introduced bacterial cells in soils under a variety of simulated environmental conditions.  相似文献   

16.
Abstract

In a fertilizer trial in a Valencia orange orchard, two soil types were distinguished: a poorly aerated loamy sand (A) and sandy clay loam (B). Mineral composition of leaves and fruit quality characteristics differed significantly in the two soil types, regardless of the fertilizer treatments. Fruits on the sandy clay loam were larger, had a thicker peel, and contained more total acids and vitamin C in the juice, and were less affected by a peel disorder (creasing). The leaves of such trees contained more K and Mg and less Ca and P.  相似文献   

17.
Abstract

Variations in the plant growth media were achieved by combining kaolinite clay (<lμm esd.), silt (2–50μm esd.) and sand (100–250 μm esd.) in various ratios. Peds of different sizes were separated from an Okolona clay soil and used as a growth media. A layer (3 cm thickness) of the sand, silt or clay and their combinations were intercalated between sandy loam soil material in a lucite coated cardboard carton. After 21 days the plants were harvested and analyzed for a number of growth parameters and related to the physical and micromorphology of the central control layer.

Germination and emergence of sorghum seed were delayed in the finer aggregates. An increase in aggregate size increased the root elongation. An examination of thin sections showed that most of the roots in the finer aggregates were grown in interpedal regions whereas in the larger aggregates roots were found in both the intrapedal as well as interpedal regions.

An increase in clay content of the central layer reduced the root growth. Silt also reduced root growth but not to the extent of the clay. Maximum root growth and penetration occurred in the mixture containing about 50 percent sand. Better root growth was observed in a sandy to sandy loam texture than clay to clay loam texture.  相似文献   

18.
不同质地土壤上玉米养分吸收和分配特征   总被引：8，自引：2，他引：8  
采用池栽试验在4种质地土壤和2种施肥水平下,研究了玉米植株氮、磷、钾的吸收和分配特征。结果表明,不同质地土壤上玉米植株养分累积量为氮钾磷;不同处理玉米氮、磷、钾的累积量表现为中壤轻壤粘壤砂壤,且各处理间差异达显著水平。不同处理叶片和茎鞘中三元素累积量的变化与单株一致,氮、磷、钾分配以叶片中的比例略高于茎鞘。从后期氮、磷、钾的转移率看,不同质地土壤表现为砂壤轻壤中壤粘壤;不同处理中子粒中氮、磷、钾的含量与产量变化一致,且处理间达显著水平。施肥增加了各种质地土壤上玉米植株及器官氮、磷、钾的累积量和子粒产量,其中砂壤增加幅度最大,轻壤和中壤次之,粘壤最小。同时施肥使得叶片和茎鞘中的氮、磷、钾转移率略有降低。  相似文献   

19.
Soils from two field experiments on straw disposal were fractionated according to particle size using ultrasonic dispersion and gravity-sedimentation in water. Samples of whole soils, clay. silt and sand-size fractions were held for 49 days at 20°C and the CO2 evolution measured on 14 dates by gas chromatography.Recovery of soil solids. C and N was 99, 98 and 93%, respectively. Most of the soil C and N was in the clay (<2μm). (loamy sand, 50% C and 56% N; sandy loam. 65% C and 68% N), the silt (2–20 μm) having smaller proportions (loamy sand, 41% C and 38% N; sandy loam. 29% C and 27% N). The sand fraction (20–6000 μm) accounted for 4–7% of the organic matter, and 1–2% of the C was water soluble. Straw incorporation generally increased the C and N content of whole soils and size fractions.The decomposition rate constants were higher for the sandy loam than for the loamy sand soil. For both soils, the decomposability of the organic matter decreased in the order: sand > clay ⩾ whole soil > silt. Straw incorporation increased the decomposition rate of whole soil and sand organic matter. whereas the effect of straw on clay and silt respiration was small.Between 58 and 73% of the respiration was from clay, 21–25% from silt and 6–19% from the sand size fraction.  相似文献   

20.
《Geoderma》2007,137(3-4):327-339
Our objectives were to describe the field-scale horizontal and vertical spatial variability of soil physical properties and their relations to soil map units in typical southeastern USA coastal plain soils, and to identify the soil properties, or clusters of properties, that defined most of the variability within the field. The study was conducted on a 12-ha field in Kinston, NC. A 1:2400 scale soil survey had delineated three soil map units in the field: Norfolk loamy sand, Goldsboro loamy sand, and Lynchburg sandy loam. These are representative of millions of hectares of farmland in the Coastal Plain of the southeastern USA. Sixty soil cores were taken to ∼ 1-m depth, sectioned into five depth increments, and analyzed for: soil texture as percentage sand, silt, and clay; soil water content (SWC) at − 33 and − 1500 kPa; plant available water (PAW); saturated hydraulic conductivity (Ksat); bulk density (BD); and total porosity. A penetrometer was used to measure cone index (CI) at each sample location. Variography, two mixed-model analyses, and principal components analysis were conducted. Results indicated that soil physical properties could be divided into two categories. The first category described the majority of the within-field variability and included particle size distribution (soil texture), SWC, PAW, and CI. These characteristics showed horizontal spatial structure that was captured by soil map units and especially by the division between sandy loams and finer loam soils. The second class of variables included BD, total porosity, and Ksat. These properties were not spatially correlated in the field and were unrelated to soil map unit. These findings support the hypothesis that coastal plain soil map units that delineate boundaries between sandy loams versus finer loam soils may be useful for developing management zones for site-specific crop management.  相似文献   

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