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1.
陆国盈  蒋明明  韩世健  裴铁雄  汤雪莲  秦洪波 《安徽农业科学》2010,38(32):18107-18109,18131
[目的]研究不同供水量与施磷量对甘蔗经济性状的耦合效应。[方法]以广西甘蔗品种新台糖22号（ROC22）为材料,将供水量设置3个水平,即在甘蔗分蘖初期至伸长末期,每旬供水量分别为199.5（A1）、400.5（A2）和600.0 m3/hm2（A3）;磷肥施用量设置4个水平,即作为基肥分别施用P2O50（B1）、120（B2）、240（B3）和360 kg/hm2（B4）,研究不同供水水平与磷肥水平组合处理对甘蔗产量和品质性状的影响。[结果]处理A2B2的水磷配比为较适合提高甘蔗经济性状。[结论]该研究为广西甘蔗生产中水分和磷肥的高效利用及高产高糖甘蔗栽培提供了理论依据。  相似文献   

2.
干旱胁迫对苗期甘蔗叶片水分和叶绿素荧光参数的影响   总被引：6，自引：0，他引：6  
[目的]研究干旱胁迫对甘蔗叶片水分和叶绿素荧光参数的影响,为甘蔗生产及评价研究提供依据.[方法]选取7个抗旱性不同的甘蔗品种,在苗期进行干旱胁迫,并测定胁迫条件下甘蔗叶片水分含量和叶绿素荧光参数的变化.[结果]甘蔗叶片水势和相对含水量与土壤相对含水量存在一定的内在联系,耐旱强的品种对土壤水分的利用率较高;相关分析和因子分析表明茁期干旱存活率、Fc/Fm、叶片水势和相对含水量可被用作抗旱性评价指标.[结论]水势表现为一个相对独立的影响因子,对甘蔗抗早性有支配作用,并验证了Fv/Fm作为甘蔗抗旱评价指标的可靠性. Abstract： [Objective] The aim was to explore the effects of water stress on leaf water and chlorophyll fluorescence parameters of sugarcane seedling,as well as to provide basis for the study on sugarcane production and evaluation.[Method]Seven different sugarcane varieties were studied at the seedling stage under drought stress,and the changes of leaf water and chlorophyll fluorescence parameters under stress conditions were detected. [Result] leaf water potential,leaf relative water content and soil relative water content showed a certain amount of internal relationship,the sugarcane varieties that had more tolerant to drought had higher utilization rate of soil water;the correlation analysis and factor analysis suggested that the survival rate at seedling stage under drought stress,Fv/Fm,leaf water potential and relative water content could be used as drought resistance evaluation indicators. [Conclusion] As a relatively independent influencing factor,water potential had dominating effect on drought resistance,and the reliability of Fv/Fm as drought resistance evaluation indicator had been verified.  相似文献   

3.
Soybean cultivar Bei 92-28 was tested in this experiment in 2000 to study the coupling effect of water and ferilizer on soybean yield. The results showed that the effect of irrigation varied among the levels of fertilizer application,and vice versa；pods per plant ,seeds per pod. and 100-seed weight had positive correlations with soybean yield,but the degrees of correlations of different treatments were various;LAI and dry matter accumulation could be significantly increased when watered and applied fertilizer with different levels,but high fertilizer application treatment didn‘t obtain the highest yield; watering could increase the absolute absorption amount of N,P,K in seeds,but the accumulation rates were various.  相似文献   

4.
Scarcity of water and nutrients in orchards are the major causes of low productivity and decline of citrus in tropics. With a hypothesis that the optimal amount of water and nutrients application through drip irrigation(DI) could save substantial irrigation and fertilizers comparative to that under traditional basin irrigation(BI) with band placement of fertilizer(BPF), this study was conducted in citrus in vertisol of central India. Three DI regimes: I_1, I_2 and I_3 scheduled at 50% class-A pan evaporation rate(E_p), 75% E_p, and 100% E_p along with three fertilizer doses: F_1, F2 and F_3 at 50% recommended dose of fertilizer(RDF, N:P_2O_5:K_2O=600 g:200 g:100 g), 75% of RDF and 100% of RDF were applied to mandarin plants respectively. BI with band placement of fertilizer(BPF) at 100% RDF was taken for comparison. All the drip-fertigation treatments(except I_1F_1) produced higher plant growth and fruit yield, with better quality fruits compared with BI+BPF. The highest fruit yield(16.39 t ha~(–1)), water use efficiency(3.9 kg m~(–3)) and fertilizer use efficiency(87.3 kg kg~(–1)) were observed in I_2F_2. The concentrations of N, K and Fe in leaves were significantly higher in I_2F_2 compared to other treatments. The leaf photosynthesis, stomatal conductance(Gs) and transpiration were the highest in I_3F_3. However, leaf water use efficiency(LWUE, photosynthesis/transpiration) was the highest in I_2F_2. The overall results of this study demonstrated that the application of optimum quantity of water and fertilizers(I_2F_2) through DI could be a productive and water efficient option in citrus production in vertisol.  相似文献   

5.
A pot experiment was conducted under water deficit and adequate water-supplied conditions with two maize genetypic varieties （Shaandan 9 and Shaandan 911） to study the effects of nitrogen, potassium and glycinebetaine （GlyBet） on the dry matter and grain yields as well as water use efficiency （WUE）. Determinations were made at different stages of the two varieties for revealing the function of these factors in increasing plant resistance to drought. Results showed that under a water-stressed condition, dry matter and grain yield were significantly reduced. However, the response of the two varieties to water stress was different： Shaandan 9 was significantly higher in dry matter and grain yields, and therefore could be regarded as a drought-resistant variety compared to Shaandan 911.Application of nitrogen, potassium and glycinebetaine raised dry matter and grain yield to different levels, and thereby alleviated the water stress and increased water use efficiency. These effects were higher for Shaandan 911 than for Shaandan 9. Under water-stressed conditions application of N fertilizer, either at low rate or at high rate, significantly increased dry matter, grain yield and water use efficiency. A significant different effect was found for Shaandan 911 between N rates, but not so for Shaandan 9. However, with supplemental water supply, effects of N fertilization were obviously decreased, showing that in addition to supplying nutrient, N fertilizer has a function in increasing drought-resistance of the crop. Potassium and glycinebetaine exhibited a remarkable function in increasing dry matter and grain yields as well as water use efficiency under water stress while such effects were obviously declined, even vanished, with supplemental water supply, indicating the important contribution of these factors in rise of drought-resistance ability of a crop.  相似文献   

6.
The determination method of Schisandrin A and Schisandrin B in Schisandra chinensis was improved with the high performance liquid chromagraphy(HPLC).The sample was extracted exceedingly in the critical limit of CO 2 .The retention time of Schisandrin A and Schisandrin B was reduced,with methano/water(75:25)as mobile phase.The wavelength for detection was 254 nm. The R2 of standard curve was 0.9998 and the relative standard deviation was 2.31%and 3.17%with the recovery of 96.45%and 97.37%,respectively.The result shows that the rate of veracity of this method is higher and it proves that the determination method of Schisandrin A and Schisandrin B in Schisandra chinensis is a feasible method.  相似文献   

7.
To achieve the purpose of reducing farm non-point source pollution, we integrated site specific nitrogen management precise irrigation, controlled drainage, and wetland eco-repair system in dike area of Taihu basin. During investigation, it had given prominence for the water and fertilizer coupling effects of precise irrigation and site specific nutrient management, the characteristics of integration on controlled irrigation, controlled drainage and wetland ecosystem non-point source pollution control. Then the water and fertilizer integrated management mode of paddy field was put forward in Taihu basin where the water production efficiency increased to 1.64 kg. m-3, water saved 37.8%, fertilizer use efficiency raised 15,4%, yield raised 10%, and N, P load decreased 26%-72%. The modern agricultural and farmland ecosystems that control and cut down the farm non-point source pollution came into being, which can be a reference by Taihu basin to control its agricultural non-point source pollution and eutrophicated water body.  相似文献   

8.
聚乙烯醇空心微球的制备及其对尿素的缓释作用(英文)   总被引：2，自引：0，他引：2  
[Objective] The aim of this study was to discuss the optimizing preparation conditions of polyvinyl alcohol(PVA)hollow microsphere and its application in the production of slow-release urea fertilizer.[Method]PVA hollow microsphere was prepared by the emulsion chemical cross-linking method,while its composition,morphology and particle size was analyzed by technologies of FT-IR,SEM and TEM respectively.Thus,factors such as rate of emulsified speed,crosslink temperature and linking agent amount with effects on morphology and particle size of hollow microsphere were also discussed in this study.Furthermore,based on the optimizing preparation conditions,PVA fertilizer carrier microsphere was prepared by coating urea to investigate its sustained release effect on urea.[Result]The optimizing preparation conditions of polyvinyl alcohol(PVA)hollow microsphere were as follows:rate of emulsified speed 6 000 r/min,crosslink temperature 35 ℃ and linking agent amount 25 ml.PVA fertilizer carrier microsphere had significant sustained release effect on urea,and the optimal cross-linking time was 3 hours.[Conclusion]This study provides theoretical basis for the development of new slow-release fertilizer.  相似文献   

9.
[Objective]The toxicity effect of Hg2 and Cr(Ⅵ)on alga growth in eutrophic water was studied to provide reference for biomonitoring and bioremediation of eutrophic water.[Method]The mother liquid of alga,which was separated from the eutrophic water,were put into the solutions of Hg2 and Cr(Ⅵ)with different concentrations and their mixture solution,respectively.And the toxicity effect of Hg2 and Cr(Ⅵ)on the growth and propagation of alga in eutrophic water was observed.[Result]The alga in eutrophic water performed rather sensitive to Cr(Ⅵ)and when its concentration Was over 1 ms/L,threre was obvious effect on alga growth.The alga Was not very sensitive to Hg2 when its concentration was lower, but its toxicity became stronger and stronger when its concentration increased to some extent and the toxicity effect of Cr(Ⅵ)on alga growth was inst on the centrary. When the ion concentration was lower than 10 ms/L,the toxicity of Hg2 on alga was lower than that of Cr(Ⅵ).When the concentration was over 10 ms/L,the toxieity of Hg2 exceeded that of Cr(Ⅵ).The toxicity of ion mixture solution of Hg2 and Cr(Ⅵ)had synergism inhibition on alga,which could be performed only when the concentration was over4 mg/L.[Conclusion]The toxicity of heavy metal on alga not only related to the alga cell,but also related to the concentration of heavy metal ion.  相似文献   

10.
The objective of this study was to investigate the effects of applying different amounts of water and nitrogen on yield, fruit quality, water use efficiency(WUE), irrigation water use efficiency(IWUE) and nitrogen use efficiency(NUE) of drip-irrigated greenhouse tomatoes in northwestern China. The plants were irrigated every seven days at various proportions of 20-cm pan evaporation(E_p). The experiment consisted of three irrigation levels(I1, 50% E_p; I2, 75% E_p; and I3, 100% E_p) and three N application levels(N1, 150 kg N ha~(–1); N2, 250 kg N ha~(–1); and N3, 350 kg N ha~(–1)). Tomato yield increased with the amount of applied irrigation water in I2 and then decreased in I3. WUE and IWUE were the highest in I1. WUE was 16.5% lower in I2 than that in I1, but yield was 26.6% higher in I2 than that in I1. Tomato yield, WUE, and IWUE were significantly higher in N2 than that in N1 and N3. NUE decreased with increasing N levels but NUE increased with increase the amount of water applied. Increasing both water and N levels increased the foliar net photosynthetic rate. I1 and I2 treatments significantly increased the contents of total soluble solids(TSS), vitamin C(VC), lycopene, soluble sugars(SS), and organic acids(OA) and the sugar:acid ratio in the fruit and decreased the nitrate content. TSS, VC, lycopene, and SS contents were the highest in N2. The harvest index(HI) was the highest in I2N2. I2N2 provided the optimal combination of tomato yield, fruit quality, and WUE. The irrigation and fertilisation regime of 75% E_p and 250 kg N ha~(–1) was the best strategy of water and N management for the production of drip-irrigated greenhouse tomato.  相似文献   

11.
底栖鱼类对水田上覆水中磷素动态的扰动效应   总被引：2，自引：0，他引：2  
[目的]研究底栖鱼类泥鳅对水田上覆水中磷素动态的扰动效应,探讨生物扰动机制.[方法]基于模拟试验,使用离子色谱法和分光光度法,对比分析上覆水中磷素含量在有/无泥鳅活动时的差异.[结果]扰动组的TP、DTP和PP浓度在试验开始阶段与对照组无显著差异,在试验中、后期显著高于对照(P<0.05).扰动组要的PP/TP高于对照组,扰动组中TP浓度的增加主要是由于PP的增加,扰动组的DIP/DT在试验中、后期显著高于对照(P<0.05).[结论]底栖鱼类对水田上覆水中的磷素产生了扰动作用,增加了水稻生长可利用的的磷素养分. Abstract： [Objective] The research aimed to investigate the bioturbation effects of benthic fish Misgurnus anguillicaudatus on phosphorus dynamic in overlying water of paddy field,as well as to explore the bioturbation mechanism.[Method]Based on simulation experiment,the phosphorus contents in overlying water were analyzed comparatively with and without Misgurnus anguillicaudatus by the using of ion chromatography and spectrophotometry. [Result] The concentrations of total phosphorus (TP),dissolved total phosphorus(DTP)and particular phosphorus(PP) in bioturbation group had no significant differences with those in control group in initial stage of experiment,and became significantly higher than control group in middle and late stages of experiment(P<0.05).The PP/TP ratios in bioturbation group were bigger than those in control group,the increase of TP concentration in bioturbation group was mainly due to the increase of PP.The ratios of dissolved inorganic phosphorus(DIP) to DTP (DIP/DTP) were significantly bigger than those in control group in middle and late stages of experiment (P<0.05). [Conclusion] The benthic fish had bioturbation effects on phosphorus in overlying water of paddy field,which increased the available phosphorus for rice growth.  相似文献   

12.
[目的]构建抗黄瓜花叶病毒RNAi载体,并将载体转入烟草.[方法]采用RT-PCR方法,扩增黄瓜花叶病毒NS04加工番茄分离物的RNA2基因组的序列选取CMV RAN2基因组中的复制酶片段作为靶序列,构建pBi35SCR2真核表达载体,并对表达载体时行鉴定;通过农杆菌介导的方法将表达载体转入烟草,用PCR的方法检测载体是否转入.[结果]系统进化树分析结果表明,RNA2中编码CMV-2a的序列与中国浙江的DQ412731 分离物有较高核苷本乡酸及氨基酸同源性,分别达到98.0%和96.5%;RCR结果表明,试验成功构建了pBi35SCR2真核表达载体,并成功将表达载体转入烟草[结论]试验获得的转基因烟草可作为后期攻毒试验的材料,并为研究加工番茄抗黄瓜花叶病毒奠定了基础. Abstract： [Objective] Aimed to construct RNAi vector resistant to cucumber mosaic virus and transferred this vector into tobacco.[Method]RT-PCR method was used to amplify cucumber mosaic virus NSO4 and process RNA2 gene sequen of tomato isolates.The analysis results of phylogenetic tree demonstrated that the sequence in RNA2 encoded CMV-2a had 98.0% and 96.5% homology with nucleotide and amino acid of DQ412731 isolate of Zhejiang,China.The replicase fragment in CMV RAN2 gene was taken as target sequence to construct pBi35SCR2 eukaryotic expression vector,then the expression vector was identified.Through agrobacterium-mediated method,the expression vector was transferred into tabacco and PCR method was used to check the transfer.The PCR results demonstrated that the experiment had successfully construct eukaryotic expression vector of pBi35SCR2 and the expression vector was successfully transferred into tabacco. [Conclusion] The obtained transgenic tobacco could be used as challenge test material in following experiment and provided foundation for studying processing tomato resist cucumber mosaic virus.  相似文献   

13.
牛体外发育胚胎特定阶段差异表达基因的研究   总被引：1，自引：0，他引：1  
[目的]研究不同发育时期牛体外受精胚胎在基因表达模式上的差异.[方法]利用单个胚胎mRNA差异显示技术,对单个8细胞期胚胎与囊胚进行mRNA差异显示,获得1条特异表达条带,对其进行克隆、测序,并与GenBank进行对比.[结果]该序列与牛核糖体蛋白131基因(ribosomal protein 131,RPL3])具有99%的同源性.采用实时定量PCR技术检测8细胞期和囊胚期胚胎RPL31的mRNA表达量,结果表明,RPL31在8细胞期胚胎的相对表达量为囊胚期胚胎的3.2倍.[结论]为揭示和阐明控制牛早期胚胎发育的相关机理提供依据. Abstract： The cattle different stage embryos obtained from in vitro was studied using the technology of single preimplantation embryo mRNA different display:single 8-cell and blastocyst stage embryos were studied using technology of mRNA different display and one different fragment was found.The result suggested that this fragment displayed high homology (99%) to cattle mRNA for ribosomal protein L31.Then to detect the expression of RPL31 mRNA in 8 cell and blastocyst stage embryos by real-time quantitative PCR,the result showed the relative amount of 8 cells was 3.2 times of blastocyst's.  相似文献   

14.
一株产紫杉醇的曼地亚红豆杉内生真菌的分离及鉴定   总被引：1，自引：0，他引：1  
[目的]分离并鉴定1株产紫杉醇的曼地亚红豆杉内的内生真菌.[方法]从曼地亚红豆杉树皮内表皮中分离得到32株内生真菌,并通过高效液相色谱法检测其发酵产物.[结果]筛选获得1株可以产紫杉醇的内生真菌M57,其紫杉醇产量为45～50μg/L,并通过对M57菌落的形态学观察以及18S rDNA序列分析初步将其鉴定为根霉属(Rhizopus)真菌.[结论]该菌株的发现为微生物发酵法生产紫杉醇提供了具有潜在应用价值新的菌种. Abstract： [Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media.[Method]32 strains of endophytic fungi were identified form the inner bark of T.media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp.through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp.M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.  相似文献   

15.
依据最新NDB数据库中蛋白质-DNA复合物晶体结构数据,基于修正的DNA结构统计力学模型,利用蒙特卡洛多重积分计算DNA动力学结构的有关参数,并对计算得到的结果进行时间复杂度和精确度分析. Abstract： Based on protein-DNA complex crystal structural data in up-to-date Nucleic Acid Database,the related parameters of DNA Kinetic Structure were investigated by Monte-Carlo Multiple Integrals on the base of modified DNA structure statistical mechanical model,and time complexity and precision were analyzed on the calculated results.  相似文献   

16.
电场作用下人工湿地植物的生理生化响应   总被引：1，自引：0，他引：1  
通过测定人工湿地植物叶片中叶绿素含量、MDA含量及SOD酶活性.研究不同通电强度下人工湿地植物生理特性变化,分析电场对植物生理特性的影响机理,为利用电场强化人工湿地去除污染物提供依据.研究结果表明,与对照植物相比,1和3 V低强度电压对植物正常生理指标变化无明显影响,且生长趋势优于对照组;随着电压强度的升高,植物叶片中叶绿素含量、MDA含量以及SOD酶活性受到较大影响,表明植物体受到较强的氧化胁迫,生长受到危害.合适的电场能够强化人工湿地的污水处理效果. Abstract： By measuring wetland plants chlorophyll content,malondialdehyde (MDA) content and superoxide dismutase (SOD) enzyme activity,the changes of wetland plant physiological characteristics under different power strength were studied,and the mechanism of electric field on plant physiological characteristics was analyzed to provide a theoretical basis for the pollutant removal ability strengthening of artificial wetland under electric field.The results showed that compared with the control plants,low-intensity-voltage (1 V and 3 V) had no significant effect on the normal physiological and biochemical indexes of the plants,and the growth trend was better than the control group;with the voltage increasing,plant chlorophyll content,MDA content and SOD activity were greatly affected,indicating that plants were under strong oxidative stress,and the growth was damaged.Therefore,a suitable electric field could enhance the sewage treatment effect of constructed wetland.  相似文献   

17.
中草药提取液对黄瓜苗期杀根结线虫的活性研究   总被引：1，自引：0，他引：1  
[目的]研究中草药提取液对黄瓜苗期杀根结线虫的活性.[方法]利用植物水培技术,结合生长条件易控制、根系生长过程易连续观察的特点,应用5种具有较强室内触杀根结线虫活性的植物提取液及其与阿维菌素的复配液,对黄瓜苗期水培根系杀线活性进行系统研究.[结果]胡黄连和石榴皮提取液可以在不影响水培黄瓜苗期植株生长的前提下表现出对根结线虫良好的活体植株根系寄生防治和杀灭活性,且达到了与阿维菌素相近的作用水平;而狗脊、木香和蛇床子提取液对黄瓜的生长表现出不同程度的抑制作用,其对根结线虫的防治效用和杀灭活性也较弱.[结论]触杀效果和杀线活性成分能否被植物吸收利用共同制约着具有杀线活性中草药提取液的开发应用. Abstract： Present mature plants hydroponic technology was used,combined with some excellent characteristics,such as growth conditions was easy to control and process of root growth was easy to continuously observe,the nematicidal activity of 5 kinds of Chinese herbs extracts and the compound solution of Avermectin,with strong contact toxicity effect indoor,was systematically studied and investigated the affection on the rootknot nematode parasitized on the cucumber seeding stage.It is found that under the premise of no influence on root growth of cucumber,extracts from Picrorhiza scrophulariiflora and Punica granatum showed strong prevention and nematicidal activity,and had the similar efficacy of Avermectin;while the extracts from Cibotium barornetz,Aucklandia lappa Decne and Fructus cnidii showed low nematicidal activity and various degrees inhibition effect on plant growth.  相似文献   

18.
铅镉复合污染对水稻生长及产量的影响   总被引：4，自引：0，他引：4  
[目的]探讨铅镉复合污染对水稻生长、产量及品质的伤害及机理.采用盆栽试验,研究了不同程度的铅镉复合污染对水稻生长发育及产量品质的影响.[结果]铅镉低浓度胁迫能够促进水稻生长,高浓度抑制水稻的株高;铅镉胁迫显著降低了水稻的分蘖数,随着胁迫浓度增加,分蘖数下降幅度增大;铅镉胁迫显著降低了水稻每盆穗数、结实率、千粒重和产量,低浓度胁迫下每穗总粒数显著增加,高浓度胁迫下每穗总粒数显著降低;铅镉低浓度胁迫下糙米绿和精米率显著增加,高浓度胁迫下糙米率和精米率显著降低,整精米率随处理浓度的增加而显著下降;水稻籽粒中铅镉含量与土壤中铅镉浓度成显著正相关,土壤铅镉含量达到中高浓度时,水稻籽粒铅镉含量严重超标,铅镉最高超标达333%和122%.[结论]该研究为污染地区水稻栽培技术体系的建立提供依据. Abstract： [Objective] The aim was to study the damage and the mechanism of combined pollution of Pb and Cd on rice growth,yield and quality of rice.[Method]Effects of combined pollution of Pb and Cd on the growth and yield of rice were studied by pot experiment with cultivating rice. [Result] Pb and Cd could promote dce growth at low concentration while inhibit plant height at high concentration;Pb and Cd stress reduced the number of rice tillers significantly,with the increasing of stress concentration,the decrease amplitude of the number of tillers was greater;the panicle per pot,seed setting rate,1000-grain weight and yield of rice were reduced significantly by Pb and Cd stress,the grain number per panicle decreased significantly under low concentration of Pb and Cd but increased significantly under high concentration;the brown rice rate and milled rice rate increased significantly under low concentration of Pb and Cd while at high concentration of Pb and Cd,the both two reduced significantly,the head milled rice rate reduced significantly with the decreasing of concentration;The Pb and Cd content of grains were significantly positively related to the concentrations of Pb and Cd content in soil,it had excessively exceeded hygienic standard when the concentrations of Pb and Cd in soil were medium-high and the unqualified rate reached 333% and 122%.[Condusion]The study had provided basis for the establishment of rice cultivation system in pollution areas.  相似文献   

19.
[目的]研究催产素(OT)在广西本当水牛下丘脑、垂体及卵巢的分布上的联系,进而了解OT在下丘脑、垂体及卵巢3者之间分泌释放途径.[方法]采用免疫组织化学SuperPicTureTM二步法检测5头广西本地水牛的下丘脑、垂体和卵巢中催产素(OT)的分布情况.[结果]下丘脑中分泌OT的神经元主要分布在弓状核、视上核及室旁核,在腹内侧核、腹外侧核、交叉上核、背内侧核、乳头体、下丘脑前核等核团也有一定数量的阳性神经元;在腺垂体发现OT免疫反应阳性产物,自垂体柄和正中隆起的一侧可见到平行排列的OT阳性神经纤维断续地延伸至神经部;卵巢中只在生殖上皮、卵泡颗粒细胞和黄体细胞发现有大量OT免疫阳性产物.[结论]首次发现OT存在于广西本地水牛下丘脑-垂体-卵巢轴的各个环节,并且下丘脑中各主要核团均有OT免疫阳性神经元的分布,尤其以弓状核、视上核、室旁核分布最多,为进一步研究OT的合成和作用机制提供形态学依据,并对广西本地水牛的繁殖育种及泌乳起到一定的参考指导作用. Abstract： To study the association of oxytocin (OT)'s distribution in hypothalamatic,pituitary and ovary,and understand how the OT secrete releasing in hypothalamus,pituitary and ovaries,the paraffin section immunohistochemistry SuparPicTureTM two step method was used to detect the distribution of OT in hypothalamatic-pituitary-ovary axis of five femal Guangxi local buffalo.The test results could provide morphology according to study the OT's synthesis and mechanism of action ,and could play reference and directions part in breeding Guangxi local buffalo.The test results display:oxytocin immuno reactive (OT-IR) neuronsw eremainly distributed arcuate nucleus,supraoptic nucleus and paraventricular nucleus,and OT-IR neurons was also found in ventrornedial nucleus,ventrolateralis nucleus,suprachiasrnaticus nucleus,dorsomediai nucleus,mamillary body,anterior hypothalamic nucleus and so on.The OT immunoactive production was found in pituitary and few OT-IR nerve fibers extended to post pituitary from hypophyseal stalk and medium eminence.In ovaries,OT immunoactive productions were only distributed in germinal epithelium cells,granulosa cells and lutein ceils.The OT was first discovered in singulorum link of hypothalamatic-pituitary-ovary axis of Guangxi local buffalo.The OT immunoactive neurons were first discovered in every main nucleus of Guangxi local buffalo hypothalamus,especially distributed in arcuate nucleus,supraoptic nucleus and paraventricular nucleus.  相似文献   

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