共查询到20条相似文献，搜索用时 15 毫秒
1.
Joamir Barbosa Filho Maria Angélica Di Carvalho Leandro Silva de Oliveira Enéas Ricardo Konzen Gilvano Ebling Brondani 《林业研究》2018,29(1):73-84
Khaya anthotheca is a hardwood species from Africa and recently introduced to Brazil. This species yields high-quality wood for diverse applications, but little has been done toward an effective propagation method for large-scale production in nurseries. We evaluated the effects of different concentrations of macro and micronutrients (i.e., 100, 50 and 25% of the concentration of a referenced solution) combined with indole-3-butyric acid (IBA) at 0 and 2 g L?1 on the survival rate of mini-stumps and the survival and adventitious rooting of K. anthotheca mini-cuttings. The mini-stumps were grown from a source of seeds imported from Ghana, West Africa. The mini-stumps survived at a high rate (97%). Consistently high shoot yields were obtained from the mini-stumps (average of 1.8 per mini-stump). High survival percentage of mini-cuttings and adventitious rooting were observed when solutions with 100 and 50% of the nutrients concentration were combined with 2 g L?1 IBA. Histological sections analyzed through optical microscopy indicated the tissues from mini-cutting stems were juvenile, which might have stimulated adventitious rooting. These results have important implications for further work aimed at establishing propagation strategies for K. anthotheca, which are of prime importance for assisting breeding programs of this species. 相似文献
2.
Low temperature, IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings 总被引：1，自引：0，他引：1
Gilvano Ebling Brondani Francisco José Benedini Baccarin Heron Wilhelmus de Wit Ondas José Luiz Stape Antonio Natal Gonalves Marcilio de Almeida 《林业研究》2012,23(4):583-592
Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques,such as cutting or mini-cutting.We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA(indole-3-butyric acid) concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse.Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings.For the first experiment,the mini-cuttings were stored at 4℃ for 0(immediate planting),24,48,72,96 and 120 h.The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse.The basal region of the mini-cutting was treated with various IBA solutions:0(free of IBA),1,000,2,000,3,000 and 4,000 mg·L-1.Every seven days(0(immediate planting),7,14,21 and 28 days),destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting.Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots.The 2,000 mg·L-1 IBA concentration induced a greater speed and percentage of adventitious rooting,and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse.Exposure to low temperature induced adventitious root formation with diffuse vascular connections. 相似文献
3.
Joséli Schwambach Carolina Michels Ruedell Márcia Rodrigues de Almeida Ricardo Miguel Penchel Elias Frank de Araújo Arthur G. Fett-Neto 《New Forests》2008,36(3):261-271
Eucalyptus globulus Labill and hybrids thereof have low lignin content, favoring cellulose extraction, but are often recalcitrant to clonal propagation.
This work analyzed biochemical and morphological changes during adventitious rooting of mini-cuttings of E. globulus × maidenni obtained from mini-stumps cultured in drip fertigated sand bed or intermittent flooding tray commercial propagation systems.
Morphological (% rooting, root number and length, mean rooting time) and biochemical parameters (peroxidase activity, total
phenolic content and flavonoid content) were monitored to characterize the rooting phases. All of the rooting parameters were
equivalent in both systems, indicating comparable efficiency of both methods in clonal propagation. Kinetic profiles of biochemical
parameters were also similar, although the activity of peroxidases was an order of magnitude higher and the phenolic content
about three times lower in cuttings derived from intermittent flooding-grown mini-stumps than in those derived from sand bed-grown
mini-stumps. Taken together, results suggest that rooting phases were similar in both systems: induction before day 5, formation
from day 5 to 15, and elongation from day 15 to 45. These data may contribute to the development of rooting phase-specific
mineral nutrient solutions to maximize clonal propagation and plant survival. 相似文献
4.
青钱柳子叶不定根的发生机制 总被引：3，自引：0，他引：3
本文以青钱柳子叶为材料,WPM为基本培养基,研究不同质量浓度的外源ABA和IBA对子叶不定根发生的影响,并对不定根发生与内源激素的变化关系进行探讨.结果表明:添加0.5～5 mg·L~(-1) IBA,对子叶不定根发生均有不同程度的促进作用,以1.5 mg·L~(-1)效果最佳,诱导生根率、平均生根条数和根长均达最大,生根率可达到100%;而添加0.01～0.5 mg·L~(-1) ABA和不加激素的对照没有不定根的发生.内源激素的动态测定表明IAA对不定根的发生起关键性作用,根原基的启动需要较高的内源IAA,而不定根的形成与根的伸长需要相对较低的内源IAA水平.较高的IAA/ABA,IAA/GA_3比值有利于不定根的发生,能较好反映出生根过程中内源激素间的平衡需求,GA_3可能不是影响不定根发生的关键因素.研究结果为青钱柳生根诱导及其机制的研究提供依据. 相似文献
5.
Ivar Wendling Gilvano Ebling Brondani Leonardo Ferreira Dutra Fabrício Augusto Hansel 《New Forests》2010,39(3):343-353
An efficient production system, which can quickly produce high quality plants, is shown in this study of Liquidambar styraciflua. The mini-cuttings technique was used for ex vitro clonal propagation of three sweetgum clones. The semi-hydroponics system for production of propagules was also tested, and
the concentration of indole-3-butyric acid (IBA) for rooting was evaluated. Three adult clones, produced according to conventional
cutting processes, were used as mini-stumps and maintained in semi-hydroponics systems of medium size sand. Ten collections
of sprouts were prepared to evaluate the production of semi-hydroponics systems (mini-clonal hedges). Sprouts from collection
number six were used at different concentrations of IBA solution (0; 500; 1,000; 3,000 and 6,000 mg l−1) to test the mini-cuttings technique. The high survival rates of mini-stumps after ten collections (96.3%), and high annual
production per square meter (~2,953 mini-cuttings) showed that the mini-clonal hedges are viable to conduct sweetgum mini-stumps.
The formation of 62.5% of healthy plants after hardening under outdoor conditions showed that sweetgum could be successfully
propagated by the mini-cuttings technique. 相似文献
6.
几种生根剂对金银忍冬嫩枝扦插育苗作用的试验初报 总被引：2，自引：0，他引：2
用IBA、NAA、ABT1、IBA＋NAA四种生根剂,分别以不同浓度,不同浸泡时间处理金银忍冬嫩枝插穗进行扦插育苗试验,结果表明：用IBA 1000×10^-6、处理2s、生根率最高为69.4%;用NAA 1000×10^-6、处理时间2s、生根率最高为90%;用ABT1 200×10^-6、处理时间2h、生根率最高为80.2%;用NAA 100×10^-6＋IBA 50×10^6、处理2h、生根率90%或NAA 50×10^-6＋IBA 100×10^-6、处理12h、生根率90%,用清水处理2h,生根率70.4%,表明用外源激素处理能够提高金银忍冬嫩枝扦插的生根率,增加不定根数量。从上面的方差分析结果综合生根率和生根性状的各项指标,如果选择一种激素,选择NAA 100×10^-6＋IBA50×10^-6、处理2h、生根率90%,效果最为理想。 相似文献
7.
Leaf area, IBA concentration and age of stockplants were all found to be important factors for successful rooting for vegetative
propagation using single-node cuttings of the two main African mahogany species: Khaya anthotheca and K. ivorensis. Cuttings with leaf area of 30–50 cm2 had the best rooting percentage and cuttings with about 30 cm2 had the most number of roots per cutting in K. anthotheca. Khaya ivorensis, cuttings with 10–30 cm2 leaf area had the highest rooting percentage. Cuttings collected from 1-year-old stockplants recorded the highest rooting
percentage and largest number of roots per cutting. Cuttings from 3-year-old stockplant of the same seeds sources had the
lowest rooting suggesting aging negatively impacted rooting ability. The effect of auxin concentration, on rootability was
examined with cuttings of K. anthotheca. An IBA concentration of 0.8% was the best exogenous auxin concentration for percentage rooting, number of roots per cutting
and the length of the longest root per cutting. 相似文献
8.
红叶臭椿组培快繁育苗技术研究 总被引：1，自引：0，他引：1
试验以春季新萌发的红叶臭椿幼嫩枝条为外植体，通过系列试验探讨了不同植物激素对红叶臭椿离体培养有效增殖的影响。结果表明：MS＋6-BA 1．0mg／L＋IBA 0．1mg／L利于外植体的启动培养；MS＋6-BA 0．5mg／L＋IBA 0．1mg／L是有效增殖的适宜培养基，其继代增殖系数达5．0；最佳生根培养基为1／2MS＋IAA 3．0mg／L＋IBA 2．0mg／L，生根率可达90％以上。适宜的组培苗移栽技术，使组培苗的温室移栽成活率达90％以上。并通过简化培养基的试验，极大降低了红叶臭椿组培苗的生产成本，形成一套切实可行的组培快繁育苗体系。 相似文献
9.
10.
重瓣大岩桐叶片离体培养高频植株再生体系的建立 总被引：3，自引：0，他引：3
以重瓣大岩桐叶片为外植体进行离体培养再生体系研究.结果表明:以新展开的嫩叶为外植体最好,芽分化率达96.67%;叶片通过脱分化和再分化直接产生不定芽.6-BA和NAA适宜的配比均可促进芽的诱导和增殖,IBA有利于根的生长;MS 6-BA 2.0mg/L NAA 0.2 mg/L培养基最适合芽的诱导,诱导率达96.55%;增殖培养以带芽愈伤团效果较好,在MS 6-BA 2.0mg/L NAA 0.2 mg/L LH 500 mg/L培养基中,增殖系数达18.在1/4MS IBA 0.5mg/L培养基中生根率高达100%.炼苗移栽成活率达92%. 相似文献
11.
【目的】研究不同生长调节剂处理对金叶银杏硬枝扦插生根率和成活率的影响,探讨金叶银杏的生根机制,为金叶银杏苗木扦插快繁技术体系的建立提供技术支持和理论指导。【方法】通过NAA和IBA处理金叶银杏硬枝插穗,检测它们对插穗的生根状态、生根指标及IBA对扦插过程中插穗皮部可溶性糖、抗氧化酶、激素含量的影响,探究金叶银杏硬枝扦插繁殖技术和生根机理。【结果】1)金叶银杏插穗愈伤组织诱导期和形成期、不定根发生期和形成期分别在插后的20~26、26~42、42~53、53~79 d。2)800 mg·L^-1 IBA和400 mg·L^-1 NAA+400 mg·L^-1IBA(混合)处理的插穗愈伤组织和不定根出现期及生根率与根数都较其他相应处理的效果好,其中生根率分别达64.4%和51.1%;不定根数分别达8.6条和9.3条。3)插穗生根过程中(500 mg·L^-1 IBA处理)基部韧皮部的SOD、PPO活性在插后40、60 d达到高峰,均比对照提前20 d达到峰值;插穗的POD活性出现上升-下降-升高的趋势,插穗可溶性糖也出现升高-下降-升高的趋势,但时间点不一致;而淀粉的含量出现下降-上升的趋势。4)生根期间插穗基部韧皮部的IAA含量出现了2个峰值和一个谷值;而ZR含量在第40天达到峰值后下降;GA含量先下降后上升;ABA含量在插后的前期高后期低,且值都低于同期对照。IAA/ABA比值在插后的第40天(愈伤组织形成期)达到谷底,后又上升(不定根形成期)。IAA/GA在插后的40~60 d内快速上升,80 d后又开始大幅下降;IAA/ZR值在扦插愈伤组织形成期(40 d)呈下降趋势,到60 d时(不定根发生期)又急剧上升到峰值。【结论】高活性的SOD和POD有利于插穗初期愈伤组织的形成(40 d),高活性的POD与PPO有利于不定根的形成(60 d)。较高的IAA含量是保证愈伤组织形成(20~40 d)的必要条件,不定根形成期(60 d)需要较高的IAA/ZR、IAA/ABA、IAA/GA比值。本研究为金叶银杏扦插繁殖技术和生根机理的研究及金叶银杏苗木的快速繁殖生产提供技术支持。 相似文献
12.
《林业研究》2017,(1)
We developed a shoot multiplication protocol for Syringa reticulata Blume var. mandshurica Hara from in vitro cultured seedlings that derived from in vitro germinated seeds. The shoots could be induced on Murashige and Skoog(MS) medium with proper plant growth regulator combinations of 6-benzylaminopurine(BA) and indole-3-butyric acid(IBA). The better medium for shoot multiplication and growth was MS + 5 mg L~(-1)BA +0.5 mg L~(-1)IBA + 20 g L~(-1)sucrose +7 g L~(-1)agar, and the corresponding shoot induction rate was 75 %. The plantlets grew well after rooting on 1/2MS medium(macro-elements of MS medium are at half-strength) supplemented with 1 mg L~(-1)IBA, and the survival percentage was 80 % at 16 weeks after transplanting. 相似文献
13.
IBA是较易人工合成的生长素之一,对插条基部不定根的产生有促进作用,常用做生根剂。通过使用IBA处理黄刺玫等12种绿化植物的嫩枝试验研究,结果表明:IBA2000 mg·L-1速蘸嫩枝能明显提高白玉山蔷薇等8种绿化植物的生根率;在条件有限时,可以不对木槿进行药剂处理;对锦带花药剂处理差异不显著;对黄刺梅、天女木兰应寻求提高二者生根率的更优处理。 相似文献
14.
樟树组织培养快繁育苗技术研究 总被引：3，自引：1，他引：2
文章报道了应用组织培养育苗技术对樟树家系进行快速繁殖的初步结果.试验表明:带腋芽的茎段外植体在添加细胞分裂素和生长素的MS培养基上可分化形成不定芽,但芽增殖和继代培养过程并不需要添加生长素.在DCR BA 3.0 mg/L的培养基上增殖培养时,平均每个外植体增殖4.7个不定芽,高度小于1.5 cm的不定芽的比例为74.1%;而在DCR BA 4.0 mg/L的培养基上培养时,平均每个外植体增殖2.4个不定芽,高度大于1.5 cm的枝芽比例可提高至58.1%.枝芽接种在DCR IBA 1.5 mg/L的培养基上诱导生根培养28 d的生根率为98%,移植的再生植株95%以上存活. 相似文献
15.
福建山樱花扦插繁殖及其影响因子研究 总被引：13，自引：2，他引：13
在塑料拱棚下进行福建山樱花半木质化枝条扦插繁殖试验,分别进行了不同扦插繁殖时间、不同浓度的ABT1号和IBA处理、不同扦插基质和添加钙镁磷对生根促进剂提高福建山樱花扦插生根的影响试验及综合试验。结果表明,福建山樱花半木质化枝条扦插繁殖以冬末春初为适宜时期,以2份素沙+1份粘性黄土为扦插基质,插穗经IBA 2 500 mg.L-1+钙镁磷沾根I、BA 3 750 mg.L-1+钙镁磷沾根或IBA 5 000 mg.L-1浸泡处理均有良好的促进生根效果,平均插穗生根率和平均一级不定根数最高分别可达86.67%和4.68条。 相似文献
16.
重瓣非洲菊的组培技术研究 总被引：5，自引：0，他引：5
取重瓣非洲菊幼小花托 ,以MS为基本培养基 ,附加不同生长调节剂及浓度水平诱导愈伤组织形成不定芽进行组培研究。结果表明 :重瓣非洲菊幼小花托在MS +BA 5 0mg·L-1+NAA 0 5mg·L-1培养基上能很好地诱导形成愈伤组织并分化出不定芽 ,诱导率 91 8% ;MS +KT 5 0mg·L-1+IAA 0 5mg·L-1培养基对不定芽增殖效果较好 ,增殖倍数达4 4 4 ;生根培养基为 1/ 2MS +NAA 1 0mg·L-1,生根率达 98%。选择河泥 +细沙 (2∶1)混合作移栽基质 ,成活率达 90 %以上。 相似文献
17.
A.T.M. Abdullah M.A. Hossain M.K. Bhuiyan 《林业研究》2006,17(4):301-304
Introduction Guava (Psidium guajava Linn.), belonging to the Family Myrtaceae, is originated in the tropical South America (Hayes 1970; Pathak and Ojha 1993) and grows wild in Bangladesh, India, Thailand, Brazil, Florida, West Indies, California and also … 相似文献
18.
惠园橄榄扦插繁殖育苗技术的研究 总被引：2，自引：0，他引：2
分年度对惠园橄榄品种扦插繁殖的生根及生长能力与插穗年龄、生根促进剂、扦插时间、扦插基质、插穗长度等相关因素进行研究,结果表明:①扦插后50d开始形成不定根;②插穗年龄、生根促进剂、扦插时间、扦插基质和插穗长度对插穗的生根及生长能力都有显著影响;③选用2年生已充分木质化的枝条作插穗,激素以100×10-6IAA+IBA浸基24h效果最好,基质以细河沙+黄心土(1∶1)为佳,成活率可达89 6%。 相似文献
19.
Many plantation tree species are cloned to achieve the growth, disease resistance and wood quality characteristics required
for a successful economic venture. However, clonal propagation is limited by declines in adventitious root formation with
increasing stock plant age. We examined the effects of immediate or delayed IBA application on adventitious root formation
and subsequent root and shoot development of cuttings harvested from 8-year-old clonal hedge plants of Pinus elliottii var. elliottii × P. caribaea var. hondurensis. IBA applied at the time of setting accelerated root formation, elevating the percentage of cuttings with roots at 13 weeks
post-setting from 45 to 78% and from 83 to 93% for a low- and a high-rooting clone, respectively. Final rooting percentages
for the same treatments and clones (78 and 85%, and 88 and 100%, respectively, at 20 weeks post-setting) were not significantly
affected by IBA application. IBA increased the root:shoot ratio of rooted cuttings by decreasing shoot weight compared with
untreated cuttings, without affecting root weight, root length, root surface area or root volume. IBA was only effective when
applied at the time of setting. A simple IBA treatment for cuttings from 8-year-old clonal hedges, by accelerating root production,
has potential for reducing nursery costs and increasing the root system quality of containerised pine cuttings. 相似文献
20.
试验用不同质量浓度的IBA、IAA和NAA溶液处理伞房决明的1年生硬枝。结果表明,①不同质量浓度的外源生长素促根效果不同。IBA的6种浓度处理的促根效果都比对照好;IAA的各种处理效果不明显;NAA 25 mg.L-1的促根效果比对照好,其它浓度处理不如对照好。②在供试的3种外源生长素中,以吲哚丁酸的促根效果最好,其中1 000 mg.L-1处理5 min,500 mg.L-1处理10 min,250 mg.L-1处理30 min和100 mg.L-1处理120 min的促根效果都很突出。 相似文献