共查询到20条相似文献，搜索用时 250 毫秒
1.
宁夏温室黄瓜新病害靶斑病的药剂筛选 总被引：2，自引：0，他引：2
宁夏温室新病害黄瓜靶斑病的防治技术研究明确了几种杀菌剂对黄瓜靶斑病的防治效果.结果表明:3％中生菌素可湿性粉剂22.5 g/hm2、47％春雷·王铜可湿性粉剂662.7 g/hm2、50％烯酰吗啉可湿性粉剂225.0 g/hm2、25％嘧菌酯悬乳剂112.5 g/hm2药后7d防治黄瓜靶斑病的效果在55.49％～67.72％之间,药后12 d防治黄瓜靶斑病的效果在83.41％～89.30％之间.说明这几种杀菌剂对黄瓜靶斑病具有较好的防治效果,黄瓜生长正常,没有药害发生. 相似文献
2.
3.
4.
5.
玉米叶螨和大小斑病防治试验 总被引：1，自引：0，他引：1
经过试验，筛选出4种药效快、持效期长的防治玉米叶螨的药剂，药后10天防效为86．8％－92．6％，增产率16．4％－24．75％；筛选出4种防治玉米大小斑病的药剂，其防效为53％－66．9％，增产率12．3％－27．5％；摘除玉米底叶对玉米叶螨和大小斑病有一定防治作用。 相似文献
6.
于彦懿 《内蒙古农业大学学报(自然科学版)》1989,10(1):127-130
本文首次明确呼市蔬菜公司菜库在冷藏期间造成蒜苔严重腐烂和损失的主要病害种类。根据诱发产生的病原物镜检,初步鉴定主要是青霉菌(Penicillium sp.)占病斑数的33％,丝核菌(Rhizoctonia sp.) 占病斑数的30％,疫霉菌(Phytophthora sp.)占病斑数的10％,未产生病征的病斑占27％。对冷藏蒜苔发病后可能造成的危害程度和最大贮存期限的估测,提出以湿腐的流行速度和从有效积温予测的探讨。并对冷藏蒜苔控制病的发展提出了防治建议。 相似文献
7.
“９２８１”防治苹果树皮腐烂病的室内及田间试验结果表明,“９２８１”对苹果树皮腐烂病有明显的治疗效果。“９２８１”室内抑菌效果明显,用药液浸泡病组织,４倍液杀菌率８４．０％,原液杀菌率１００％．平板抑菌试验５００倍液抑菌率为１００％．日间涂刷病斑后有明显的治疗和铲除病菌的作用。用原液,２倍液、４倍液涂刷病斑５０ｄ后观察,可明显抑制病斑扩展,防效在９０％以上。 相似文献
8.
应用8种化学农药对福建锥栗溃疡病进行化学防治,结果表明,不同药剂不同浓度处理后,锥栗溃疡病病斑抑制率、病斑愈合率和绝对防治率均存在显著差异。不同处理中,25％溃疡灵可湿性粉剂200、400倍液和80％代森锌可湿性粉剂200倍液的绝对防治率较好,分别达到869．6％、633．4％和385．3％。8种药剂的3种浓度中,80... 相似文献
9.
果园冬季管理是果树丰产的基础。笔者认为果园冬管应主要注意以下一些方面。 一要防治苹果树腐烂病,随着果树进入休眠期,腐烂病发病率剧增,而且病变向皮内深层发展。防治方法主要是及时重刮病斑,而后再涂药。其药液配方如下:40％福美砷1份,煤油1份、洗衣粉0.2份和水30份。可用刀在病斑上划线,深达木质部,间隔1厘米,涂上药液一次;或在病斑部位 相似文献
10.
11.
采用室内抑菌试验与田间喷雾、病斑涂抹和灌根等试验相结合的方法,研究了防治猕猴桃溃疡病的有效药剂种类及其优化施用技术。结果表明,95%CT原粉、60%DTMzWP和1000万单位农用链霉素的室内抑菌效果较好;田间以95%CT原粉500倍液刮除病斑涂药防治效果最好,治愈率达94.3%,其次为DTMZ、农用链霉素和菌毒清;不同施药方法组合中,以喷雾+刮除病斑涂抹组合的防治效果最好,最高防效可达96%;秋季采用注射法,可有效预防翌年早春猕猴桃溃疡病的发生危害。 相似文献
12.
限根栽培对设施大樱桃幼树控冠效果的研究 总被引：3，自引：0，他引：3
为解决设施大樱桃树冠生长旺盛、结果晚、产量低的问题,采用槽式、箱筐式以及结合根系断根修剪的限根技术,研究了不同限根方式对设施大樱桃幼树的控冠效果。结果表明,环形断根抑制株高和新梢生长的作用大于平行行向两侧纵向断根的抑制作用,其中环形断根抑制株高首年和次年分别为20.4%和19.1%,平行行向两侧纵向断根抑制株高首年和次年分别为18.3%和11.4%;环形断根抑制新梢生长长度首年和次年分别为23.7%和25.3%,平行行向两侧纵向断根抑制新梢长度首年和次年分别为18.4%和15.3%。限根栽培第2年,环形断根和平行行向两侧纵向断根处理每株花芽形成数量分别高于对照51.6%和61.7%,每株花束状果枝数分别高于对照84.6%和67.0%。限根栽培改变了树体内赤霉素水平。研究认为,限根栽培明显抑制设施大樱桃的生长,并能显著提高设施大樱桃花芽分化数量;设施大樱桃限根栽培方式可以采用断根修剪处理和箱框式限根处理,断根处理的限根栽培方式以平行行向距主干30cm处两侧纵向断根为宜。 相似文献
13.
转反义蜡质基因纯合株系间直链淀粉含量与RVA谱关系分析 总被引：2，自引：0，他引：2
【目的】排除以往水稻亚种或品种间RVA谱分析时存在着的遗传背景差异,分析转反义蜡质基因不同直链淀粉含量纯合株系间RVA各特征值的差异,更清楚地揭示直链淀粉含量与RVA谱特征值的关系。【方法】采用根癌农杆菌介导共转化和花药培养相结合的方法,培育含有反义蜡质基因的花培纯系,并对各株系进行直链淀粉含量和RVA谱测定和分析,通过比较RVA谱曲线图形及RVA谱特征值的显著性测定,分析相同遗传背景条件下直链淀粉含量对各RVA谱特征值的作用。【结果】在武运粳7号转基因后代中,获得了只含反义蜡质基因、不含潮霉素抗性标记的花培株系77个,经T1H1和T1H2二代直链淀粉含量测定,有19个株系的直链淀粉含量接近对照（对照16.0%）;有50个株系直链淀粉含量比对照下降1%～5%,其中,直链淀粉含量在11.0%～13.9%有30个株系;另外,有8个株系的籽粒为蜡质状,直链淀粉含量降至3.1%～4.0%。不同直链淀粉含量纯系间RVA谱分析发现存在二种RVA谱曲线图形,直链淀粉含量在3.1%～4.0%株系明显不同其它株系,也不同于常规糯稻;对RVA谱曲线图形相同、直链淀粉含量不同的株系进行分类比较,低直链淀粉含量株系的RVA谱曲线最终表现为曲线逐步上升,但没有超越第1个峰值。对不同直链淀粉含量株系类群的各RVA谱特征值进行显著性测定,3.1%～4.0%株系群有5个特征值与其它二个株系群存在极显著差异;11.0%～13.5%株系群有3个特征值与对照16.0%组存在极显著差异;直链淀粉含量与RVA谱各特征值之间优化数学模型显示：峰值时间和消减值与直链淀粉含量关系较为密切。【结论】导入反义蜡质基因,能导致直链淀粉含量降低;通过花药培养能快速获得纯合株系;直链淀粉含量差异不仅影响到各RVA谱特征值,而且还造成RVA谱曲线不同。 相似文献
14.
《农业科学学报》2010,(8)
Anther culture pure lines with antisense Wx gene were generated by Agrobacterium tumefaciens-mediated co-transformation followed by anther culture in transgenic rice(Oryza sativa L.ssp.japonica).The antisense Wx transgenic pure lines were used to analyze the differences and the relationship between RVA eigenvalues among the lines with different amylose contents.77 pure lines of anther culture having antisense Wx gene were studied for the amylose content of its offspring cultivar,Wuyunjing 7.It was found that levels of amylose content were similar to the control in 19 lines(16.0%);1-5% lower than control in 50 lines,of which,within 11.0-13.9% in 40 lines;and 3.1-4.0% in 8 lines.The effect of the amylose content on RVA eigenvalue was analyzed through the RVA profile graphic comparison and test of significance for RVA eigenvalues.The analysis of RVA profiles with different amylose contents indicated that there were two kinds of RVA profiles within genetically similar cultivars.The lines with 3.1-4.0% of amylose content were distinctly different from other lines and conventional glutinous rice.The comparison of the similarity curve of RVA profile of the lines with different amylose contents showed that the lines with lower amylose content had a distinguished RVA profile with the curve increasing gradually,but not exceeding the first apex.The test of significance for eigenvalues of the RVA profile in the lines with different amylose contents indicated that there were five eigenvalues remarkably different among the lines with 3.1-4.0% of amylose content compared with other two groups.Further,there were three eigenvalues remarkably different among the lines with 11.0-13.5% of amylose content compared with control group.An optimized mathematical model,which characterizes the relationships between the amylose content and RVA eigenvalue,has been established in this study.From this model,it was clearly indicated that the parameters PeT and SBV were more related to amylose content.It was concluded that the differences in amylose content not only affected the eigenvalues of RVA but also caused different RVA profiles within genetically similar cultivars.The introduction of antisense Wx gene could lead to reduce the amylose content and lay theoretical foundation for quality improvement in rice breeding programs. 相似文献
15.
Anther culture pure lines with antisense Wx gene were generated by Agrobacterium tumefaciens-mediated co-transformation followed by anther culture in transgenic rice (Oryza sativa L.ssp.japonica).The antisense Wx transgenic pure lines were used to analyze the differences and the relationship between RVA eigenvalues among the lines with different amylose contents.77 pure lines of anther culture having antisense Wx gene were studied for the amylose content of its offspring cultivar,Wuyunjing 7.It was found that levels of amylose content were similar to the control in 19 lines (16.0%);1-5%lower than control in 50 lines,of which,within 11.0-13.9% in 40 lines;and 3.1-4.0% in 8 lines.The effect of the amylose content on RVA eigenvalue was analyzed through the RVA profile graphic comparison and test of significance for RVA eigenvalues.The analysis of RVA profiles with different amylose contents indicated that there were two kinds of RVA profiles within genetically similar cultivars.The lines with 3.1-4.0% of amylose content were distinctly different from other lines and conventional glutinous rice.The comparison of the similarity curve of RVA profile of the lines with different amylose contents showed that the lines with lower amylose content had a distinguished RVA profile with the curve increasing gradually,but not exceeding the first apex.The test of significance for eigenvalues of the RVA profile in the lines with different amylose contents indicated that there were five eigenvalues remarkably different among the lines with 3.1-4.0% of amylose content compared with other two groups.Further,there were three eigenvalues remarkably different among the lines with 11.0-13.5% of amylose content compared with control group.An optimized mathematical model,which characterizes the relationships between the amylose content and RVA eigenvalue,has been established in this study.From this model,it was clearly indicated that the parameters PeT and SBV were more related to amylose content.It was concluded that the differences in amylose content not only affected the eigenvalues of RVA but also caused different RVA profiles within genetically similar cultivars.The introduction of antisense Wx gene could lead to reduce the amylose content and lay theoretical foundation for quality improvement in rice breeding programs. 相似文献
16.
Anther culture pure lines with antisense Wx gene were generated by Agrobacterium tumefaciens-mediated co-transformation followed by anther culture in transgenic rice （Oryza sativa L. ssp. japonica）. The antisense Wx transgenic pure lines were used to analyze the differences and the relationship between RVA eigenvalues among the lines with different amylose contents. 77 pure lines of anther culture having antisense Wx gene were studied for the amylose content of its offspring cultivar, Wuyunjing 7. It was found that levels ofamylose content were similar to the control in 19 lines （16.0%）; 1-5% lower than control in 50 lines, of which, within 11.0-13.9% in 40 lines; and 3.1-4.0% in 8 lines. The effect of the amylose content on RVA eigenvalue was analyzed through the RVA profile graphic comparison and test of significance for RVA eigenvalues. The analysis of RVA profiles with different amylose contents indicated that there were two kinds of RVA profiles within genetically similar cultivars. The lines with 3.1-4.0% of amylose content were distinctly different from other lines and conventional glutinous rice. The comparison of the similarity curve of RVA profile of the lines with different amylose contents showed that the lines with lower amylose content had a distinguished RVA profile with the curve increasing gradually, but not exceeding the first apex. The test of significance for eigenvalues of the RVA profile in the lines with different amylose contents indicated that there were five eigenvalues remarkably different among the lines with 3.1-4.0% of amylose content compared with other two groups. Further, there were three eigenvalues remarkably different among the lines with 11.0-13.5% of amylose content compared with control group. An optimized mathematical model, which characterizes the relationships between the amylose content and RVA eigenvalue, has been established in this study. From this model, it was clearly indicated that the parameters PeT and SBV were more related to amylose content. It was concluded that the differences in amylose content not only affected the eigenvalues of RVA but also caused different RVA profiles within genetically similar cultivars. The introduction of antisense Wx gene could lead to reduce the amylose content and lay theoretical foundation for quality improvement in rice breeding programs. 相似文献
17.
18.
马铃薯sAGP基因表达对块茎淀粉和还原糖含量的影响 总被引：5，自引：0，他引：5
通过根癌农杆菌（Agrobacterium tumefaciens）介导将CaMV 35S驱动的正/反义sAGP基因导入马铃薯（Solanum tuberosum L.）品种鄂马铃薯3号（E3）和南中552（N552）以研究sAGP基因对马铃薯块茎淀粉-糖代谢的影响。PCR扩增和Southern杂交证明正/反义sAGP基因已整合到马铃薯基因组中。Northern杂交分析表明，该正/反义基因在转基因植株中均能正常转录。转基因植株块茎分析显示，导入正义基因的株系中除4个与转反义基因表现相同外，其余19个株系AGPase酶活性平均上升约25％，还原糖含量下降20％左右，淀粉含量增加5%～6％。其中3个测定指标与各自对照相比，差异均达到显著水平的有A8、C1和C5，最大变化幅度分别为酶活性上升53.5％，还原糖含量下降60.1％，淀粉含量增加33.9％。转反义sAGP基因的15个株系中，AGPase酶活性平均下降约27％，还原糖含量上升近36％，淀粉含量下降亦达27％，其中株系B5、B6、B8和D7的这3个指标均与各自未转基因对照差异显著。本研究结果表明，sAGP基因与AGPase酶活性直接相关，而AGPase很大程度上调节着马铃薯块茎淀粉-糖代谢，通过增强sAGP表达，能有效改良块茎的加工品质。 相似文献
19.
转基因高油酸甘蓝型油菜新种质的获得 总被引：7，自引：1，他引：6
在获得转fad2基因ihpRNA的甘蓝型油菜的基础上,应用气相色谱技术分析了转基因株系W-4(T_1代)单株自交种子(T_2代)的脂肪酸组成.结果显示:由49个单株组成的T:代群体油酸(C_(18:1))含量变幅为55.87%84.73%,其中有19个(占38.8%)单株种子油酸含量大于75%,有11个(占22.4%)单株种子油酸含量大于80%;受体(对照)的油酸含量变幅为55.28%～67.43%;获得的转基因高油酸材料(油酸含量≥75%)种子中亚油酸(C_(18:2))和亚麻酸(C(18:3))含量明显低于受体;高油酸材料的油酸脱饱和指数ODP值为5%~12%,而受体的ODP值高达20%～36%.基于ODP值,可以推测转基因高油酸材料种子中的FAD2活性受到有效抑制,从而阻止了油酸向亚油酸、亚麻酸的合成. 相似文献
20.
药剂诱导孤雌生殖在玉米育种中的应用研究 总被引：1，自引：0，他引：1
用二甲亚砜、青鲜素和秋水仙碱等药物处理269份未授粉的玉米雌穗花丝,诱导孤雌生殖获得结实,并从中选出11个较好的纯合二倍体.鉴定表明,这些纯系在纯度、遗传稳定性和配合力等方面与常规自交系相似.纯系所配的杂交种比对照烟单14号增产1.9～12.6%.穗长、穗粗、穗粒数的平均杂种优势分别比对照种高10.3%、4.7%和24.0%. 相似文献