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1.
In order to evaluate the impact of no-tillage (NT) against conventional-tillage (CT), or the effect of different fertilizers and herbicides, soils recently incorporated to agriculture from the El Salado basin river (Buenos Aires, Argentina) under a corn (1996/1998)-pasture (1998-)rotation were monitored for several soil (micro)biological, physical and chemical properties during the second (1997) to fourth (1999) years from the beginning of the experiment (1996). The rates of exchange of O2 and CO2 expressed in terms of kg O2 or CO2–C per hectare per day, are denoted as qO2 and qCO2. They decreased at the end of the corn growth cycle (T2) along with an increase of the respiratory quotient (RQ=qCO2/qO2), when compared to the time of land preparation (T0), irrespective of the management system. Both, qO2 and qCO2, increased 8 months after the mixed-grass-legume pasture planting (T3) keeping similar RQs with values slightly higher for NT than CT. At T4 (after 20 months of pasture), a 22 and 21% decrease of organic carbon was registered for NT and CT, respectively, with respect to the beginning of the experiment. A 22% (not significative) and a significative 31% diminish of total nitrogen was observed in the NT and CT systems, respectively. The results obtained are discussed in terms of soil function and sustainability in both management systems tested.  相似文献   

2.
Woody plant proliferation in grasslands and savannas has been documented worldwide in recent history. To better understand the consequences of this vegetation change for the C-cycle, we measured soil microbial biomass carbon (Cmic) in remnant grasslands (time 0) and woody plant stands ranging in age from 10 to 130 years in a subtropical ecosystem undergoing succession from grassland to woodlands dominated by N-fixing trees. We also determined the ratio of SMB-C to soil organic carbon (Cmic/Corg) as an indicator of soil organic matter quality or availability, and the metabolic quotient (qCO2) as a measure of microbial efficiency. Soil organic carbon (Corg) and soil total nitrogen (STN) increased up to 200% in the 0–15 cm depth increment following woody plant invasion of grassland, but changed little at 15–30 cm. Cmic at 0–15 cm increased linearly with time following woody plant encroachment and ranged from 400 mg C kg−1 soil in remnant grasslands up to 600–1000 mg C kg−1 soil in older (>60 years) woody plant stands. Cmic at 15–30 cm also increased linearly with time, ranging from 100 mg C kg−1 soil in remnant grasslands to 400–700 mg C kg−1 soil in older wooded areas. These changes in Cmic in wooded areas were correlated with concurrent changes in stores of C and N in soils, roots, and litter. The Cmic/Corg ratio at 0–15 cm decreased with increasing woody plant stand age from 6% in grasslands to <4% in older woodlands suggesting that woody litter may be less suitable as a microbial substrate compared with grassland litter. In addition, higher qCO2 values in woodlands (0.8 mg CO2-C g−1 Cmic h−1) relative to remnant grasslands (0.4 mg CO2-C g−1 Cmic h−1) indicated that more respiration was required per unit of Cmic in wooded areas than in grasslands. Observed increases in Corg and STN following woody plant encroachment in this ecosystem may be a function of both greater inputs of poor quality C that is relatively resistant to decay, and the decreased ability of soil microbes to decompose this organic matter. We suggest that increases in the size and activity of Cmic following woody plant encroachment may result in: (a) alterations in competitive interactions and successional processes due to changes in nutrient dynamics, (b) enhanced formation and maintenance of soil physical structures that promote Corg sequestration, and/or (c) increased trace gas fluxes that have the potential to influence atmospheric chemistry and the climate system at regional to global scales.  相似文献   

3.
Microorganisms are the regulators of decomposition processes occurring in soil, they also constitute a labile fraction of potentially available N. Microbial mineralization and nutrient cycling could be affected through altered plant inputs at elevated CO2. An understanding of microbial biomass and microbial activity in response to belowground processes induced by elevated CO2 is thus crucial in order to predict the long-term response of ecosystems to climatic changes. Microbial biomass, microbial respiration, inorganic N, extractable P and six enzymatic activities related to C, N, P and S cycling (β-glucosidase, cellulase, chitinase, protease, acid phosphatase and arylsulphatase) were investigated in soils of a poplar plantation exposed to elevated CO2. Clones of Populus alba, Populus nigra and Populus x euramericana were grown in six 314 m2 plots treated either with atmospheric (control) or enriched (550 μmol mol−1 CO2) CO2 concentration with FACE technology (free-air CO2 enrichment). Chemical and biochemical parameters were monitored throughout a year in soil samples collected at five sampling dates starting from Autumn 2000 to Autumn 2001.

The aim of the present work was: (1) to determine if CO2 enrichment induces modifications to soil microbial pool size and metabolism, (2) to test how the seasonal fluctuations of soil biochemical properties and CO2 level interact, (3) to evaluate if microbial nutrient acquisition activity is changed under elevated CO2.

CO2 enrichment significantly affected soil nutrient content and three enzyme activities: acid phosphatase, chitinase and arylsulphatase, indicators of nutrient acquisition activity. Microbial biomass increased by a 16% under elevated CO2. All soil biochemical properties were significantly affected by the temporal variability and the interaction between time and CO2 level significantly influenced β-glucosidase activity and microbial respiration. Data on arylsulphatase and chitinase activity suggest a possible shift of microbial population in favour of fungi induced by the FACE treatment.  相似文献   


4.
Anthropogenic conversion of primary forest to pasture for cattle production is still frequent in the Amazon Basin. Practices adopted by ranchers to restore productivity to degraded pasture have the potential to alter soil N availability and N gas losses from soils. We examined short-term (35 days) effects of tillage prior to pasture re-establishment on soil N availability, CO2, NO and N2O fluxes and microbial biomass C and N under degraded pasture at Nova Vida ranch, Rondônia, Brazilian Amazon. We collected soil samples and measured gas fluxes in tilled and control (non tilled pasture) 12 times at equally spaced intervals during October 2001 to quantify the effect of tillage. Maximum soil NH4+ and NO3 pools were 13.2 and 6.3 kg N ha−1 respectively after tillage compared to 0.24 and 6.3 kg N ha−1 in the control. Carbon dioxide flux ranged from 118 to 181 mg C–CO2 m2 h−1 in the control (non-tilled) and from 110 to 235 mg C–CO2 m2 h−1 when tilled. Microbial biomass C varied from 365 to 461 μg g−1 in the control and from 248 to 535 μg g−1 when tilled. The values for N2O fluxes ranged from 1.22 to 96.9 μg N m−2 h−1 in the tilled plots with a maximum 3 days after the second tilling. Variability in NO flux in the control and when tilled was consistent with previous measures of NO emissions from pasture at Nova Vida. When tilled, the NO/N2O ratio remained <1 after the first tilling suggesting that denitrification dominated N cycling. The effects of tilling on microbial parameters were less clear, except for a decrease in qCO2 and an increase in microbial biomass C/N immediately after tilling. Our results suggest that restoration of degraded pastures with tillage will lead to less C matter, at least initially. Further long-term research is needed.  相似文献   

5.
Management of crop residues and soil organic matter is of primary importance in maintaining soil fertility and productivity and in minimizing agricultural impact on the environment. Our objective was to determine the effects of traffic and tillage on short-term carbon dioxide (CO2) and water (H2O) fluxes from a representative soil in the southeastern Coastal Plain (USA). The study was conducted on a Norfolk loamy sand (FAO classification, Luxic Ferralsols; USDA classification, fine-loamy siliceous, thermic Typic Kandiudults) cropped to a corn (Zea mays L.) — soybean (Glycine max (L.) Merr) rotation with a crimson clover (Trifolium incarnatum L.) winter cover crop for eight years. Experimental variables were with and without traffic under conventional tillage (CT) (disk harrow twice, chisel plow, field cultivator) and no tillage (NT) arranged in a split-plot design with four replicates. A wide-frame tractive vehicle enabled tillage without wheel traffic. Short-term CO2 and H2O fluxes were measured with a large portable chamber. Gas exchange measurements were made on both CT and NT at various times associated with tillage and irrigation events. Tillage-induced CO2 and H2O fluxes were larger than corresponding fluxes from untilled soil. Irrigation caused the CO2 fluxes to increase rapidly from both tillage systems, suggesting that soil gas fluxes were initially limited by lack of water. Tillage-induced CO2 and H2O fluxes were consistently higher than under NT. Cumulative CO2 flux from CT at the end of 80 h was nearly three times larger than from NT while the corresponding H2O loss was 1.6 times larger. Traffic had no significant effects on the magnitude of CO2 fluxes, possibly reflecting this soil’s natural tendency to reconsolidate. The immediate impact of intensive surface tillage of sandy soils on gaseous carbon loss was larger than traffic effects and suggests a need to develop new management practices for enhanced soil carbon and water management for these sensitive soils.  相似文献   

6.
Conservation farming practices are often considered effective measures to increase soil organic C (SOC) sequestration and/or to reduce CO2 emissions resulting from farm machinery operation. The long-term CO2 mitigation potentials of no-till (NT) versus conventional till (CT), stubble retention (SR) versus stubble burning (SB) and N fertilisation (NF) versus no N application (N0) as well as their interactions were examined on a Vertosol (Vertisol) in semi-arid subtropical Queensland, Australia by taking into account their impacts on SOC content, crop residue C storage, on-farm fossil fuel consumption and CO2 emissions associated with N fertiliser application. The experimental site had been cropped with wheat (Triticum aestivum L.) or barley (Hordeum vulgare L.) with a summer fallow for 33 years.

Where NT, SR or NF was applied alone, no significant effect on SOC was found in the 0–10, 10–20 and 0–20 cm depths. Nonetheless, the treatment effects in the 0–10 cm depth were interactive and maximum SOC sequestration was achieved under the NT + SR + NF treatment. Carbon storage in crop residues decreased substantially during the fallow period, to a range between 0.4 Mg CO2-e ha−1 under the CT + SB + NF treatment and 2.4 Mg CO2-e ha−1 under the NT + SR + N0 treatment (CO2-e stands for CO2 equivalent). The cumulative fossil fuel CO2 emission over 33 years was estimated to be 2.2 Mg CO2-e ha−1 less under NT than under CT systems. Cumulative CO2 emissions from N fertiliser application amounted to 3.0 Mg CO2 ha−1. The farm-level C accounting indicated that a net C sequestration of 4.5 Mg CO2-e was achieved under the NT + SR + NF treatment, whilst net CO2 emissions ranging from 0.5 to 6.0 Mg CO2-e ha−1 over 33 years occurred under other treatments.  相似文献   


7.
Crop rotation and tillage impact microbial C dynamics, which are important for sequestering C to offset global climate change and to promote sustainable crop production. Little information is available for these processes in tropical/subtropical agroecosystems, which cover vast areas of terrestrial ecosystems. Consequently, a study of crop rotation in combination with no tillage (NT) and conventional tillage (CT) systems was conducted on an Oxisol (Typic Haplorthox) in an experiment established in 1976 at Londrina, Brazil. Soil samples were taken at 0–50, 50–100 and 100–200 mm depths in August 1997 and 1998 and evaluated for microbial biomass carbon (MBC) and mineralizable C and N. There were few differences due to crop rotation, however there were significant differences due to tillage. No tillage systems increased total C by 45%, microbial biomass by 83% and MBC:total C ratio by 23% at 0–50 mm depth over CT. C and N mineralization increased 74% with NT compared to CT systems for the 0–200 mm depth. Under NT, the metabolic quotient (CO2 evolved per unit of MBC) decreased by 32% averaged across soil depths, which suggests CT produced a microbial pool that was more metabolically active than under NT systems. These soil microbial properties were shown to be sensitive indicators of long-term tillage management under tropical conditions.  相似文献   

8.
Altered flux of labile C from plant roots into soil is thought to influence growth and maintenance of microbial communities under elevated atmospheric CO2 concentrations. We studied the abundance and function of the soil microbial community at two levels of spatial resolution to assess the response of microorganisms in the rhizosphere of the whole root system and of apical root zones of Phaseolus vulgaris L. to elevated CO2 and high or low N supply.

At the coarser resolution, microbial biomass C, basal respiration and phospholipid fatty acid (PLFA) patterns in the rhizosphere remained unaffected by elevated CO2, because the C flux from the whole root system into soil did not change [as shown by Haase, S., Neumann, G., Kania, A., Kuzyakov, Y., Römheld, V., Kandeler, E., 2007. Elevation of atmospheric CO2 and N-nutritional status modify nodulation, nodule carbon supply, and root exudation of Phaseolus vulgaris L. Soil Biology & Biochemistry 39, 2208–2221]. At a higher spatial resolution, more low-molecular-weight compounds were released from apical root zones under elevated CO2. Thus, at an early stage of plant growth (12 days after sowing), elevated CO2 induced an increase of enzyme activities (xylosidase, cellobiosidase and leucine-aminopeptidase) in the rhizosphere soil of apical root zones. At later stages of plant growth (21 days after sowing), however, enzyme activities (those above as well as N-acetyl-β-glucosaminidase and phosphatase) decreased under elevated CO2. The abundance of total and denitrifying bacteria in the rhizosphere soil of apical root zones was unaffected by CO2 elevation or N supply. Plant age seemed to be the main factor influencing the density of the bacterial community. In conclusion, the soil microbial community in the apical root zone responded to elevated CO2 by altered enzyme regulation (production and/or activity) and not by greater bacterial abundance.  相似文献   


9.
An energy analysis of three cropping systems with different intensities of soil tillage (conventional tillage, CT; ridge tillage, RT; no tillage, NT) was done in a loamy-silt soil (fulvi-calcaric Cambisol) at Legnaro, NE Italy (45°21′N, 11°58′E, 8 m above sea-level (a.s.l.), average rainfall 822 mm, average temperature 11.7°C). This and measurements of the evolution of the organic matter content in the soil also allowed the consequences to be evaluated in terms of CO2 emissions.

The weighted average energy input per hectare was directly proportional to tillage intensity (CT > RT > NT). Compared with CT, total energy savings per hectare were 10% with RT and 32% with NT. Average energy costs per unit production were fairly similar (between 4.5 and 5 MJ kg−1), with differences of 11%. The energy outputs per unit area were highest in CT for all crops, and lowest in NT. The RT outputs were on average more similar to CT (−12%). The output/input ratio tended to increase when soil tillage operations were reduced, and was 4.09, 4.18 and 4.57 for CT, RT and NT, respectively. As a consequence of fewer mechanical operations and a greater working capacity of the machines, there was lower fuel consumption and a consistently higher organic matter content in the soil with the conservation tillage methods.

These two effects result in less CO2 emission into the atmosphere (at 0°C and pressure of 101.3–103 kPa) with respect to CT, of 1190 m3 ha−1 year−1 in RT and 1553 m3 ha−1 year−1 in NT. However, the effect owing to carbon sequestration as organic matter will decline to zero over a period of years.  相似文献   


10.
In semiarid Mediterranean agroecosystems, low and erratic annual rainfall together with the widespread use of mouldboard ploughing (conventional tillage, CT), as the main traditional tillage practice, has led to a depletion of soil organic matter (SOM) and with increases in CO2 emissions from soil to the atmosphere. In this study, we evaluated the viability of conservation tillage: RT, reduced tillage (chisel and cultivator ploughing) and, especially, NT (no-tillage) to reduce short-term (from 0 to 48 h after a tillage operation) and mid-term (from 0 h to several days since tillage operation) tillage-induced CO2 emissions. The study was conducted in three long-term tillage experiments located at different sites of the Ebro river valley (NE Spain) across a precipitation gradient. Soils were classified as: Fluventic Xerocrept, Typic Xerofluvent and Xerollic Calciorthid. Soil temperature and water content were also measured in order to determine their influence on tillage-induced CO2 fluxes. The majority of the CO2 flux measured immediately after tillage ranged from 0.17 to 6 g CO2 m−2 h−1 and was from 3 to 15 times greater than the flux before tillage operations, except in NT where soil CO2 flux was low and steady during the whole study period. Mid-term CO2 emission showed a different trend depending on the time of the year in which tillage was implemented. Microclimatic soil conditions (soil temperature and water content) had little impact on soil CO2 emission following tillage. In the semiarid Mediterranean agroecosystems studied, NT had low short-term soil CO2 efflux compared with other soil tillage systems (e.g., conventional and reduced tillage) and therefore can be recommended to better manage C in soil.  相似文献   

11.
The effects of disturbance and glucose addition on N2O and CO2 emissions from a paddy soil at 45% WFPS (water-filled pore space) and at 25 °C were determined. During a 45-day incubation, disturbances with and without glucose addition were imposed 0, 1, 3, and 5 times. The total amount of glucose added to soil with 1, 3, and 5 disturbances was equal (0.6% of oven-dry soil basis). Strong nitrification occurred in the paddy soil during the incubation. Disturbance alone did not influence N2O and CO2 emissions significantly, but disturbance with glucose addition did (P < 0.01). A flush of N2O as well as CO2 was always observed following disturbance with glucose addition. The discrepancy in N2O emission between disturbance alone and disturbance with glucose addition was ascribed to the different magnitude of denitrification and/or heterotrophic nitrification. Greater cumulative emission of N2O was observed in the treatment of three disturbance times with glucose addition (4.3 mg N kg−1 soil), compared with five disturbances with glucose addition (2.5 mg N kg−1 soil) and one disturbance with glucose addition (2.5 mg N kg−1 soil). Cumulative CO2 emission was significant larger in one and three disturbances with glucose addition than that five disturbance with glucose addition. Supplies of available organic C appear to be a critical factor controlling denitrification and/or heterotrophic nitrification processes and N2O emission under relatively low moisture conditions, i.e. 45% WFPS.  相似文献   

12.
Quality of agricultural soils is largely a function of soil organic matter. Tillage and crop management impact soil organic matter dynamics by modification of the soil environment and quantity and quality of C input. We investigated changes in pools and fluxes of soil organic C (SOC) during the ninth and tenth year of cropping with various intensities under conventional disk-and-bed tillage (CT) and no tillage (NT). Soil organic C to a depth of 0.2 m increased with cropping intensity as a result of greater C input and was 10% to 30% greater under NT than under CT. Sequestration of crop-derived C input into SOC was 22±2% under NT and 9±4% under CT (mean of cropping intensities ± standard deviation of cropping systems). Greater sequestration of SOC under NT was due to a lower rate of in situ soil CO2 evolution than under CT (0.22±0.03 vs. 0.27±0.06 g CO2–C g−1 SOC yr−1). Despite a similar labile pool of SOC under NT than under CT (1.1±0.1 vs. 1.0±0.1 g mineralizable C kg−1 SOC d−1), the ratio of in situ to potential CO2 evolution was less under NT (0.56±0.03) than under CT (0.73±0.08), suggesting strong environmental controls on SOC turnover, such as temperature, moisture, and residue placement. Both increased C sequestration and a greater labile SOC pool were achieved in this low-SOC soil using NT and high-intensity cropping.  相似文献   

13.
为明确低O2高CO2贮藏环境对马铃薯块茎淀粉-糖代谢的影响,本试验以大西洋马铃薯为试验材料,研究体积分数为5%O2+2%CO2(CA1)、5%O2+4%CO2(CA2)、5%O2+6%CO2(CA3)、5%O2+8%CO2(CA4)及5%O2+10%CO2(CA5)的气体环境对马铃薯在4℃贮藏期间块茎中糖类、淀粉及淀粉-糖代谢相关酶[腺苷二磷酸葡萄糖焦磷酸化酶(AGPase)、尿苷二磷酸葡萄糖焦磷酸化酶(UGPase)、淀粉磷酸化酶(SP)及转化酶(INV)]活性的影响,并对块茎中还原糖含量的变化与淀粉-糖代谢相关因子进行相关性分析。结果表明,适宜的低O2高CO2贮藏环境可有效抑制淀粉含量、AGPase活性、UGPase活性的下降,并且能够抑制还原糖、蔗糖、果糖、葡萄糖含量及SP、INV活性的上升,CA1环境贮藏的马铃薯块茎在整个贮藏期间淀粉含量显著高于CK(P<0.05),还原糖、蔗糖、果糖及葡萄糖含量显著低于CK(P<0.05),AGPase、UGPase活性显著高于CK(P<0.05),而SP、INV活性均显著低于CK(P<0.05);相关性分析结果表明,还原糖含量与淀粉含量、AGPase活性、UGPase活性呈极显著负相关(P<0.01),与葡萄糖含量、果糖含量、SP活性及INV活性呈极显著正相关(P<0.01)。综上,马铃薯在CA1(5%O2+2%CO2)环境下贮藏可有效延缓块茎低温贮藏期间“糖化”现象的出现,AGPase、UGPase、SP、INV在贮藏过程中起到了一定的调控作用。本研究结果为加工型马铃薯的安全贮藏及明确“低温糖化”机理提供了理论依据。  相似文献   

14.
Under conventional farming practices, lime is usually applied on the soil surface and then incorporated into the soil to correct soil acidity. In no-till (NT) systems, where lime is surface applied or only incorporated into the soil to very shallow depth, lime will likely not move to where it is required within reasonable time. Consequently, lime may have to be incorporated into the soil by mechanical means. The objective of this laboratory study was to characterize the effect of lime, incorporated to different depths, on chemical and biological soil properties in a long-term NT soil. Soil samples taken from the 0–5, 0–10, and 0–20 cm depths were analyzed in incubation studies for soil pH, nitrate, CO2 respiration, and microbial biomass-C (MBC). Lime (CaCO3) was applied at rates equivalent to 0, 4.4, 8.8, and 17.6 Mg ha−1. Application of lime to both 0–10 and 0–20 cm depths increased soil pH from about 4.9 by 1, 1.7, and 2.8 units for the low, medium, and high liming rates, respectively. Soil nitrate increased over time and in proportion to liming rate, suggesting that conditions were favorable for N-mineralization and nitrification. Greater respiration rates and greater MBC found in lime-treated than in non-limed soils were attributed to higher soil pH. Faster turnover rates and increased mineralization of organic matter were found in lime-treated than in non-limed soils. These studies show that below-surface lime placement is effective for correcting soil acidity under NT and that microbial activity and nitrification can be enhanced.  相似文献   

15.
Irrigated rice fields account for 10–30% of global methane emissions. Rice plants ventilate the soil and enlarge the oxic–anoxic interface by their root system, thus supplying the necessary O2 to aerobic CH4 oxidizing bacteria (MOB). Rice plants (Oryza sativa type japonica var. Roma) were grown in microcosms in a greenhouse. The roots were sandwiched between two blocks of flooded rice field soil separated by a nylon gauze bag. A root mat developed which mimicked the dense root texture in the upper layer of a natural rice field. Flux measurements under oxic and anoxic conditions showed that CH4 was oxidized with a constant rate of 19% of the anoxically emitted CH4, suggesting that CH4 oxidation in the rhizosphere was at least sometimes limited by CH4 availability. Washed rice roots could both produce and oxidize CH4, depending upon incubation conditions. CH4 production by washed rice roots accounted for at most 10% of the CH4 emitted under anoxic conditions. Initial CH4 oxidation rates of washed roots equaled oxidation rates calculated from the difference between oxic and anoxic fluxes in situ. Oxidation rates became twice as high after an induction period of 20 h, indicating a limitation by O2 or CH4 in situ. The micro-environmental conditions near to the root mat were measured using microelectrodes for O2, redox potential and NH4+ and diffusion probes for CH4. Up to 42 μM O2 was detected in the root mat and concentrations were >2.5 μM in 45% of all measurements. In the bulk soil, no O2 was detected below 2 mm depth, but the root mat significantly increased the redox potential. Plant roots and associated bacteria decreased porewater CH4 and NH4+ concentrations. In the root mat, concentrations of dissolved CH4 were below the detection limit of our probes (<5 μM). Cell numbers of MOB increased with time in the rhizosphere and in the rhizoplane. MOB and aerobic heterotrophic bacteria (AHB) each numbered from 106 to 108 cells g−1 dry weight of soil or root biomass). Active MOB occurred near to a root mat similar to the dense root texture in the upper layer of rice fields. We speculate about O2 or CH4 limitation of MOB.  相似文献   

16.
Topsoil (0–25 cm) and subsoil (30–55 cm) samples were taken from clay soil which had been cropped with reed canarygrass (Phalaris arundinacea). After crumbling the soil into fragments <10 mm and removing visible organic debris, CO2 evolution was measured in the laboratory at four moisture contents (17, 26, 36 and 50% H2O for the topsoil and 16, 23, 31 and 41% for the subsoil) and at constant temperatures of −4, 0.3, 5, 15, 25, and temperatures fluctuating (weekly) between −4 and +5°C. Evolution of CO2 after the addition of roots or stubble of P. arundinacea to the topsoil (25°C, 36% H2O) was also studied. The CO2 evolution increased significantly with temperature and moisture. The CO2 evolution rate per unit of soil carbon was about two times higher for topsoil than for subsoil. Temperature fluctuation between −4 and +5°C did not enhance CO2 evolution significantly compared with incubation at a constant 5°C and was even lower than or not significantly different from samples at 0.3°C.  相似文献   

17.
  【目的】  研究青海高原麦秸和豆科绿肥混合添加下土壤温室气体排放规律及其碳氮转化机制,为该地区农田系统秸秆和绿肥科学利用提供依据。  【方法】  采用室内模拟试验,设无添加对照(CK)、单独添加毛叶苕子(VS)、单独添加麦秸(WS)、麦秸与毛叶苕子混合添加(VWS),共4个处理。测定了温室气体排放速率、土壤活性碳氮组分、土壤酶活性、细菌、古菌、真菌、amoA、nirK和narG基因丰度。  【结果】  VWS处理与VS处理相比,CO2和N2O的累积排放量分别减少24.8%和74.6%,CH4累积吸收量增加9.1%,综合增温潜势(global warming potential, GWP)显著降低76.1% (P<0.05);与WS处理相比,CO2累积排放量增加33.7%,CH4累积吸收量与N2O累积排放量分别降低12.0%和43.1%,GWP降低49.4%。有机物料添加可调节土壤pH,增加土壤碳氮含量。VWS处理的土壤pH显著高于CK和VS处理;土壤水溶性有机碳(DOC)和微生物量碳(SMBC)含量较VS处理分别增加了21.6%和4.9%,无机氮(Nmin)、土壤水溶性有机氮(DON)和微生物量氮(SMBN)含量分别降低了77.3%、59.5%和6.3%;土壤Nmin、DOC、DON、SMBC和SMBN含量较WS处理分别增加64.0%、22.5%、56.5%、23.2%和27.8%。VWS处理较其他处理,α-葡萄糖苷酶(AG)和β-葡萄糖苷酶(BG)活性显著提高,亚硝酸还原酶(NIR)活性显著降低。VWS处理的真菌和古菌群落丰度较WS处理分别提高83.8%和69.8%,较VS处理分别降低62.6%和20.3%;VWS处理细菌群落丰度较VS处理降低33.4%。VWS处理下,AOB amoA、nirK和narG基因丰度较VS处理分别降低56.6%、41.4%和16.3%,较WS处理分别降低30.3%、25.9%和12.0%。相关分析结果表明,CO2和CH4排放与土壤有机碳、全氮、DOC、SMBC和SMBN含量,AG、NAG和NR活性,真菌、细菌、nirK和narG基因丰度呈显著正相关;N2O排放与土壤全氮、DON、SMBC和SMBN含量、真菌、AOB amoA和nirK基因丰度呈显著正相关,与土壤pH和BG活性呈显著负相关。偏最小二乘路径模型分析(PLS-PM)表明,AOB amoA是调控土壤N2O排放的主要功能菌群。  【结论】  麦秸、毛叶苕子单独添加以及二者混合添加均可促进土壤CO2排放,减少CH4吸收。相较于二者单独添加,混合添加可通过降低土壤AOB群落丰度等途径,实现N2O减排和综合增温潜势下降,可作为青海高原旱地土壤温室气体减排的一项有效措施。  相似文献   

18.
Changes in some soil chemical, including 15N values, and biochemical properties (microbial C, FDA hydrolysis, glucosidase and urease activities) due to two tillage systems, conventional tillage (CT) and no-tillage (NT), were evaluated in an acid soil from temperate humid zone (NW of Spain) and compared with values obtained for a reference forest soil. The results showed that in the surface layer (0–5 cm depth) tillage tended to increase soil pH and to decrease organic matter levels and microbial biomass and activity values. The data also indicated that 8 years of NT, compared to CT, resulted in greater organic matter content and increased microbial biomass and activity, the changes being more pronounced for the microbial properties. Adoption of NT resulted in an increase of soil C storage of 1.24 Mg C ha−1 year−1 with regard to CT. The suitability of 15N as a potential tracer of land-use in this acid soil was also confirmed.  相似文献   

19.
采用biolog微生物鉴定系统和平板稀释法探讨了氮肥减量配施自制微生物菌剂对花椰菜产量及根际土壤微生物的影响研究。结果表明:T4(2/3倍N肥用量+微生物菌剂)、T5(3/4倍N肥用量+微生物菌剂)和T6(常规N肥用量+微生物菌剂)处理土壤细菌、放线菌和微生物总数数量分别增加了57.6%~321.54%,66.81%~83.62%和135.40%~232.07%,而土壤真菌数量降低34.08%~41.80%。与对照相比各施肥处理增加了土壤细菌占微生物总数的比例,降低了真菌和放线菌的比例,T5和T4处理效果最显著。与对照相比T4,T5和T6处理显著提高了土壤微生物群总落碳源利用能力。T4,T5和T6处理显著增加了土壤微生物群落对碳水化合物、羧酸类、聚合物类和氨基酸类碳源的代谢水平,T3(单施微生物菌剂)处理显著增加了土壤微生物群落对羧酸类和聚合物类碳源的代谢水平。T5和T6处理对增加花椰菜产量、生物量效果接近,花椰菜产量分别增加了12.35%和10.75%,地下干生物量增加了57%左右,T2(单施氮肥)和T4处理增加花椰菜产量5%左右。  相似文献   

20.
Surface accumulation of soil organic carbon (SOC) under conservation tillage has significant effects on stratification of other nutrients, on crop productivity and in ameliorating the greenhouse effect via atmospheric CO2 sequestration. A measure of SOC stratification relative to deeper soil layers has been proposed as a soil quality index. Our objective was to determine the effects of the duration of tillage practices upon the SOC and extractable P distribution with depth in Maury silt loams (Typic Paleudalfs) at similar levels of corn (Zea mays L.) productivity without P fertilization. Soil samples (0–20.0 cm in 2.5 cm increments) were collected under moldboard tillage (MT), chisel tillage (CT) and no-tillage (NT) and in surrounding tall fescue (Festuca arundinacea L.) sods selected from three tillage experiments (1–2-, 8- and 29-year durations) in Kentucky. SOC stratification was greater under conservation tillage (CT and NT) and sods than under MT. SOC and soil-test-extractable P stratification were positively related. Increasing the duration under NT caused the thickness of C stratification to increase. In NT soils, C stratification ratio (CSR) approached CSR in the nearby long-term sods with time. Conservation tillage rapidly promoted the occurrence of CSR greater than 2 while MT always resulted in values lower than 2. The rapid initial change in CSR suggests characterization of thin soil layers (i.e. 2.5 cm depth increments) is desirable under conservation tillage.  相似文献   

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