共查询到19条相似文献，搜索用时 639 毫秒
1.
应用 2 0条Sangon的随机引物 ,对6 0 Coγ射线辐照诱变所获新株系漳蕉 8号(原漳农 8号 )及其对照品种台湾北蕉 (MusAAAGrandCavendish)的总DNA进行RAPD ,结果发现有 1 2条引物扩增出 68条谱带 ,其中同源带 53条 ,占总带数的77 9% ,差异带 1 5条 ,占总带数的 2 2 1 % ;漳蕉 8号与对照多态性差异高达 2 4 6% ,且显示出稳定性遗传。其中S10 和S19的 3条差异带 ,可作为漳蕉 8号株系鉴定和防退化复壮的筛选标记。 相似文献
2.
EMS诱导小麦品种烟农15突变体的鉴定和EST-SSR分析 总被引：4，自引：2，他引：2
用EMS对小麦品种烟农15进行诱变处理,以构建突变体库、创造小麦新种质,为小麦功能基因研究和小麦遗传改良提供基础材料。经过M2代筛选和M3代鉴定,得到11个农艺性状发生明显变异的突变系,其中籽粒大小和株高2个性状的变异幅度最大。11个突变系均有复合性状突变出现,将其分为3类突变表型:8个大粒、高秆突变系;2个半矮秆突变系;1个高秆、多蘖突变系。用715个EST-SSR引物对受体烟农15和4个M3突变系进行了分析,共有14个引物对在受体和突变系间能扩增出差异条带。其中12个引物对扩增结果的差异表现为条带的有无;2个引物对表现为扩增出长度不同的差异条带。 相似文献
3.
高能混合粒子场诱发的小麦矮杆突变体的SSR分析 总被引：3，自引：2，他引：1
为了研究高能混合粒子场(CR)和γ射线两种不同处理方法诱变小麦产生的具有相同表型的突变体之间的分子差异,选用随机分布于小麦21对染色体上的114对微卫星(SSR)引物,对CR和γ射线处理冬小麦品种ZY9和ZH7获得的矮杆突变体M3代进行SSR分析。结果表明,CR处理产生的矮杆突变体的多态性位点主要分布在染色体2A、2B、2D、3D和5A上,而γ射线处理产生的矮杆突变体的多态性位点主要分布在染色体2A、2B、2D、4A和5A上;与γ射线处理相比,CR处理较容易产生扩增条带的增加和扩增条带长度的差异,不易产生扩增条带的缺失。序列分析表明,CR处理产生的变异主要是碱基的置换和插入,其中碱基T为易发生突变的碱基。CR诱变能够在DNA水平上导致小麦遗传物质变异,其诱变机制不同于γ射线,是一种有效的诱发突变新途径。 相似文献
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用ISSR分析四川苎麻品种(系)间的遗传关系及雄性不育分子标记的建立 总被引：6，自引：0，他引：6
本文从100条ISSR引物中筛选出21条引物,对8个四川苎麻品种(系)间的遗传关系进行了分析。PCR扩增结果表明,21条引物在8份材料中共扩增出86条带,平均每条引物扩增出4.1条,其中多态性位点71个,各引物扩增出的位点数3~8个不等,平均每条引物可以检测到3.4个多态性位点。聚类分析和遗传距离分析结果表明,供试品种川7、川8与其亲本遗传距离较远。3个杂交品种(川7、川8、川9)均表现特有的偏父本遗传现象。此外,本研究用ISSR引物U835筛选到了1个雄性不育分子标记,并将其扩增产物克隆测序,结果表明该序列大小为658bp。根据该序列,此标记被转化成稳定的SCAR标记,可用于苎麻雄性不育分子标记辅助育种。 相似文献
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应用RAPD技术研究4种鲍的亲缘关系 总被引：8，自引：2，他引：8
黎中宝 《中国生态农业学报》2004,12(4):60-63
应用RAPD技术研究 4种鲍的亲缘关系结果表明 ,4种鲍群体中 2 0个有效引物共扩增出 5 38条DNA带 ,平均每个引物扩增的条带数为 2 6 .9;扩增的多态性条带数 136 ,平均每个引物扩增的多态性条带数为 6 .8;多态位点百分数为 2 5 .3%。盘鲍群体和皱纹盘鲍群体之间遗传距离与遗传一致度分别为 0 .2 8和 0 .72 ,杂色鲍群体和九孔鲍群体之间遗传距离与遗传一致度分别为 0 .32和 0 .6 8。聚类分析把盘鲍群体和皱纹盘鲍群体聚为 1组 ,二者亲缘关系较近 ;杂色鲍群体和九孔鲍群体聚为 1组 ,二者亲缘关系也较近 相似文献
8.
烟草品种RAPD分子标记遗传差异研究 总被引：20，自引：0，他引：20
用２３５个随机引物对来源于中国,美国等国家的２３个烤烟和地方晒晾烟品种基因组ＤＮＡ进行了ＲＡＰＤ分析。结果表明：２５个引物可以扩增出多态性产物;利用其中１６个引物共扩增出１２８条带,其中４６条品种间表现多态性,这种多态性可以进行品种鉴定;根据多态性的带计算了品种间遗传距离,按类平均法可将２３个品种划分６类,各类内含不 地理来源和不同调制类型的品种,说明品种地理来源和调制类型差异与遗传差异没有必然的 相似文献
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小麦杂种优势群研究：I.利用RAPD标记研究小麦品种间遗传差异 总被引：24，自引：3，他引：24
选用我国的３８个冬小麦品种（系）和２个加拿大春小麦品种（系），利用ＲＡＰＤ标记进行小麦基因型之间分子标记遗传差异研究，探讨分子标记在建立小麦杂种优势种中的应用。利用５９个随机引物对４０个小麦基因型ＰＣＲ扩增结果表明，其中２９个引物（占４９％）扩增产物经琼脂糖凝胶电泳分离表现多态性，这２９个引物共扩增出１６８条带，其中７８条带（占４６．６％）具有多态性，每个引物可扩增１￣６条多态性带，平均２．７条带 相似文献
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甘蓝型油菜陕2A细胞质雄性不育的遗传及RAPD标记 总被引：8，自引：1，他引：7
本研究以甘蓝型油菜陕 2A细胞质雄性不育系、保持系和F2 分离群体为材料 ,对F2 分离群体的遗传分离情况进行分析 ,结果表明 ,可育与不育株花器存在明显差异 ,符合 3∶1的分离比例 ,因而推断细胞质雄性不育恢复基因受 1对显性基因控制。利用分离群体分组分析法(BSA) ,用 1 0 5个随机引物对细胞质雄性不育恢复基因进行RAPD分析 ,发现有 6个随机引物扩增出多态性差异谱带。引物S62 和S74在可育和不育基因池中扩增出单条特异性谱带所代表的DNA序列 ,很可能与不育系的恢复基因连锁。 相似文献
11.
Yu-Xia Yang Wei Wu You-Liang Zheng Li Chen Ren-Jian Liu Chun-Yan Huang 《Genetic Resources and Crop Evolution》2007,54(5):1043-1051
Genetic diversity and relationships among 48 safflower accessions were evaluated using 22 inter-simple sequence repeats (ISSR)
primers. A total of 429 bands were amplified, and 355 bands (about 82.7%) were polymorphic. Five to forty-one polymorphic
bands could be amplified by each primer, with an average of 16.1 polymorphic bands per primer. The results showed that the
polymorphism of the safflower germplasm was higher at the DNA level. All the 48 accessions could be distinguished by ISSR
markers and were divided into 9 groups based on ISSR GS by using UPGMA method. The genetic relationships among the accessions
from different continents were closer. Comparatively, the genetic diversity of the accessions originated from Asia was higher,
from Europe assembled. The results also showed that the genetic variation of accessions from Indian and Middle Eastern safflower
diversity centers were relatively higher. ISSR is an effective and promising marker system for detecting genetic diversity
among safflower and give some useful information on its phylogenic relationships. 相似文献
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Paula Martins-Lopes José Lima-Brito Sónia Gomes Julieta Meirinhos Luís Santos Henrique Guedes-Pinto 《Genetic Resources and Crop Evolution》2007,54(1):117-128
Thirty Portuguese and eight foreign olive (Olea europaea L.) cultivars were screened using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) markers.
Twenty RAPD primers amplified 301 reproducible bands of which 262 were polymorphic; and 17 ISSR primers amplified 204 bands
of which 180 were polymorphic. The percentage of polymorphic bands detected by ISSR and RAPD was similar (88 and 87%, respectively).
The genetic variability observed was similar in the Portuguese and foreign olive cultivars. Seven ISSR and 12 RAPD primers
were able to distinguish individually all 38 olive cultivars. Twenty specific molecular markers are now available to be converted
into Sequence Characterised Amplified Region (SCAR) markers. Relationships among Portuguese and foreign cultivars is discussed. 相似文献
13.
L. S. Rao P. Usha Rani P. S. Deshmukh P. A. Kumar S. K. Panguluri 《Genetic Resources and Crop Evolution》2007,54(6):1235-1244
Detection of genetic relationships between 19 chickpea cultivars and five accessions of its wild progenitor Cicer reticulatum Ladizinsky were investigated by using RAPD and ISSR markers. On an average, six bands per primer were observed in RAPD analysis
and 11 bands per primer in ISSR analysis. In RAPD, the wild accessions shared 77.8% polymorphic bands with chickpea cultivars,
whereas they shared 79.6% polymorphic bands in ISSR analysis. In RAPD analysis 51.7% and 50.5% polymorphic bands were observed
among wild accessions and chickpea cultivars, respectively. Similarly, 65.63% and 56.25% polymorphic bands were found in ISSR
analysis. The dendrogram developed by pooling the data of RAPD and ISSR analysis revealed that the wild accessions and the
ICCV lines showed similar pattern with the dendrogram of RAPD analysis. The ISSR analysis clearly indicated that even with
six polymorphic primers, reliable estimation of genetic diversity could be obtained, while nearly 30 primers are required
for RAPD. Moreover, RAPD can cause genotyping errors due to competition in the amplification of all RAPD fragments. The markers
generated by ISSR and RAPD assays can provide practical information for the management of genetic resources. For the selection
of good parental material in breeding programs the genetic data produced through ISSR can be used to correlate with the relationship
measures based on pedigree data and morphological traits to minimize the individual inaccuracies in chickpea. 相似文献
14.
Yi Hongyang Zhang Caibo Li Chuan Wang Jing Yu Tao Liu Yongming Cao Moju 《Genetic Resources and Crop Evolution》2021,68(5):1937-1947
Male sterility is widely utilized for hybrid seed production. In this study, two new found male sterile mutants SauS4 and SauS5 were obtained from space flighted seeds of maize inbred line RP125. Then, genetic analysis, molecular markers identification, and cytological observation were conducted to confirm their male sterile types. For genetic analysis, the above two male sterile mutants were continuously backcrossed with two maize inbred line 18Hong and RP125, and four stable male sterile lines SauS4(18Hong), SauS5(18Hong), SauS4(RP125), SauS5(RP125) were generated by six-generation backcross. Restoring and maintaining relationship analysis showed that both Hui313 and Zifeng1 didn’t rescue the male sterility SauS4(18Hong) and SauS5(18Hong). Using CMS mitochondria-specific primers for PCR detection suggested that only a 440 bp band unique to CMS-T type was amplified in SauS4(18Hong), SauS5(18Hong), SauS4(RP125), and SauS5(RP125). Sequencing results showed that these bands sequences were identical in DNA level which compared with T-urf13. Cytological observations showed that the main abortion stages of SauS4 and SauS5 were at the middle stage of uninucleate microspores under the two nuclear backgrounds of 18Hong and RP125, exhibiting the characteristics of sporophyte sterility. All the above results pointed out the two male sterile mutants SauS4 and SauS5 belonged to the CMS-T type. Interestingly, some mitochondrial genome difference between SauS4(RP125) and SauS5(RP125) were revealed by AFLP analysis.
相似文献15.
Ilaria Marotti Alessandra Bonetti Maurizio Minelli Pietro Catizone Giovenni Dinelli 《Genetic Resources and Crop Evolution》2007,54(1):175-188
Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and a semi-random PCR system were used to analyze
the genetic diversity of 16 Italian common bean landraces and their relationship to four commercial cultivars. Of the primers
tested, 8 ISSR, 6 RAPD and 7 semi-random primers produced polymorphic and reproducible DNA fragments. A higher proportion
of polymorphic bands were observed using ISSR (85%) and semi-random (90%) primers than RAPD (69%) method. The combination
of any two semi-random markers allowed the identification of all 20 bean genotypes. In contrast ISSR (except for primer (CAC)3GC) and RAPD markers appeared to be less informative as more than two markers were necessary to achieve the same diagnostic
level. Moreover, 7 ISSR, 2 RAPD and 8 semi-random exclusive bands were identified as putative population-specific markers.
Semi-random and ISSR derived dendrograms showed similar tendencies in terms of genetic relatedness, whereas clustering of
genotypes within groups was not similar when compared with the RAPD technique. Despite the different ability to resolve genetic
variation among the investigated landraces, two major clusters with less than 60% (ISSR) and 40% (RAPD and semi-random) genetic
similarity were formed with all three marker systems. The two groups were correlated with the phaseolin patterns and seed
size of the landraces. The analysis showed that the cultivar ȁ8Lingua di Fuocoȁ9 and most of the landraces (13 out of 16)
collected in Italy belong to the Andean gene pool, whereas only the three populations from Pratomagno belong to the Middle
American gene pool. 相似文献
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为获得更多用于构建甘蔗遗传连锁图谱可用的多态性SSR标记,本研究以6个高抗甘蔗褐锈病品种和6个高感甘蔗褐锈病品种为亲本,根据感病母本×抗病父本选配12个杂交组合,筛选在亲本间条带清晰、多态性明显、重复性较好的引物。结果表明,组合柳城03-1137×德蔗93-88、Mex105×粤糖00-236亲本间的多态性引物数最多,分别占总数的52.38%和47.62%;其中,10对引物(18-mSSCIR34、22-mSSCIR38、25-mSSCIR48、32-mSSCIR67、51-mSSCIR50、57-MSSCIR21、71-SMC1490CL*、73-SMC278CS*、75-SMC31CUQ*、77-SMC336BS*)在组合柳城03-1137×德蔗93-88中多态性最好,7对引物(22-mSSCIR38、25-mSSCIR48、45-mSSESTC04、51-mSSCIR50、67-mSSCIR9*、76-SMC334BS*、80-SMC486CG*)在Mex105×粤糖00-236中多态性最好,利用筛选得到的引物,更有利于构建分子遗传图谱。本研究结果为抗褐锈病新基因定位和开发与其紧密连锁的分子标记研究提供了一定的理论依据。 相似文献
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利用SRAP分子标记评价小麦三雌蕊近等基因系的遗传背景 总被引：5，自引：0，他引：5
普通小麦三雌蕊突变体(TP)具有明显的穗粒数优势,为评估该突变体在育种中的利用价值,进行了近等基因系培育。以三雌蕊突变体(TP)为供体,单雌蕊中国春、川麦28、绵阳29、内麦9号为轮回亲本,经7代回交和4代自交,初步培育出三雌蕊近等基因系CSTP、CM28TP、MY29TP和NM9TP。利用128对SRAP引物对培育的近等基因系及轮回亲本进行遗传分析,结果表明:(1)128对引物共扩增出978条谱带。其中有120对引物的扩增产物具有多态性,占所用引物的93.8%。这120对引物共扩增出638个差异谱带,占总谱带数的65.2%;(2)利用128对SRAP引物计算9个材料之间的遗传相似系数。其中中国春与CSTP的相似系数为0.9346,绵阳29与MY29TP的遗传相似系数为0.9070,川麦28与CM28TP的遗传相似系数为0.9397,内麦9号与NM9TP的遗传相似系数为0.8732;(3)通过聚类分析筛选出2对遗传相似性大于0.93的近等基因系,即CM28TP与川麦28、CSTP与中国春。 相似文献
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不同基因型刺梨及其近缘种亲缘关系的RAPD分析* 总被引：2，自引：0，他引：2
采用RAPD标记，对刺梨(Rosa roxburghii Tratt)7个基因型及8个近缘种(类型)进行鉴定和亲缘关系分析。结果表明，筛选出的16个随机引物可扩增出137条480bp到3.3kb大小的清晰。DNA片段，其中95条为多态性片段，为总数的69．3％；每个引物平均产生8．6条。由OPB-11、OPAF-16和OPW-02扩增的14条特异DNA片段，可有效地将普通刺梨7个基因型和无籽刺梨区分开来。基于遗传距离矩阵，采用IJPGMA法对15个供试样品的亲缘关系进行了聚类分析，文中还就无籽刺梨及重瓣刺梨的可能来源进行了探讨。 相似文献