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褶皱臂尾轮虫室内培养及在牙鲆育苗中的应用 总被引：3，自引：0，他引：3
研究了褶皱臂尾轮虫在不同的接种密度下的生长情况,以及轮虫强化在牙鲆早苗培育中的作用。结果表明:较高的轮虫接种密度可产生高增值数,最高培育密度2380个/ml;是否添加小球藻对轮虫生长具有明显作用;在牙鲆早苗培育中,轮虫投喂前须用乳化油强化,投喂未强化轮虫组白化率达78%,成活率仅12%。 相似文献
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(2)轮虫、蒙古裸腹蚤的培养:培养池为常用水泥池,24小时连续充气,充气量以轮虫、蒙古裸腹蚤不因缺氧而浮于水面为宜。每天投喂适量酵母和小球藻液,轮虫培养每天投喂酵母量为0.005克/万个,分4～6次投喂;蒙古裸腹蚤投喂酵母量为1～2毫克/升,分3次投喂;每天换水20%,换水后添加小球 相似文献
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利用啤酒酵母活菌株培养褶皱臂尾轮虫的研究 总被引：11，自引：0，他引：11
本文介绍了用啤酒酵母活菌株培养褶皱臂尾轮虫的试验成果。试验表明：(1)轮虫接种的适宜密度是14—70个/毫升；(2)投喂量对水环境因子有一定影响，但在充分充气条件下，pH和溶氧量变化对轮虫增殖没有明显影响，而化学耗氧量的变化在超过100ppm时。对轮虫增殖有严重影响。(3)投喂量应根据水温和轮虫的数量来确定，在水温30℃时，投喂率3(克酵母／克轮虫)，连续采收使轮虫密度保持在200个／毫升左右，可望长时间获得轮虫高产稳产的培养效果；(4)除啤酒酵母外，投喂适当经乳化处理的清鱼肝油，不会影响轮虫的产量，但可以提高轮虫作为幼鱼饵料的营养效果。 相似文献
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用酵母高密度培养褶皱臂尾轮虫 总被引：1，自引：0，他引：1
用面包酵母室内高密度培养褶皱臂尾轮虫,结果发现,1.5 g/(106·d)的较高投饵量.适合于接种密度约为50个/ml的褶皱臂尾轮虫培养,而投饵量为1.0～1.2 g/(106·d)时.适合于200个/ml以上较高密度的褶皱臂尾轮虫培养.投饵量为1.5 g/(106·d)时.不适于500个/ml以上接种密度的褶皱臂尾轮虫培养.若褶皱臂尾轮虫密度继续高至1000个/ml以上,投喂量则需进一步降低至1 0 g/(106·d)以下.褶皱臂尾轮虫接种密度低至30个/ml以下时,除酵母外,适当添加一定量(1/3以上体积)的单胞藻.可提高褶皱臂尾轮虫的增殖速度,降低褶皱臂尾轮虫培育的风险. 相似文献
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在室内水槽中进行蒙古裸腹(Moinamongolica)的大量培养,研究不同接种密度和面包酵母投喂水平对该种群变动和生产量的影响。所用蒙古裸腹为20世纪90年代采自山西省晋南半咸水湖、在海水中长期培养保存下的种。实验1中,接种密度分别设为338ind/L、145ind/L和88ind/L3个水平,面包酵母投喂量为5mg/L,每天4次,各设3个重复。实验2中,面包酵母投喂量分别为30mg/(L·d)、20mg/(L·d)和10mg/(L·d)3个水平,蒙古裸腹接种密度为900ind/L,各设3个重复。实验结果表明:(1)在一定范围内蒙古裸腹培养产量随接种密度增加而增加,当接种密度低于88ind/L时,接种的蒙古裸腹较难形成稳定的种群。(2)当使用面包酵母(Bakeryeast)作为培养蒙古裸腹的惟一食物时,食物需要量随种群密度的变化而变化。当密度低于1500ind/L时,适宜的面包酵母投喂水平为20mg/(L·d);当密度超过5000ind/L时为30mg/(L·d)。上述结果显示,接种密度和面包酵母投喂量对蒙古裸腹种群变动和生产量具有重要影响。建议在蒙古裸腹培养生产中接种密度高于150ind/L,面包酵母投喂量为0 8×10-5～1 3×10-5g/(ind·d)。 相似文献
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用干酵母作为褶皱臂尾轮虫的饵料,进行工厂化培养试验。结果表明:(1)在水温25~28℃,盐度23-25。连续充气的条件下,轮虫生长繁殖稳定;(2)经过7-10 d的培养,A、B、C三个水泥池内轮虫的密度分别从接种时的16、23、38个／ml增加到269、312、266个／ml.平均日增殖率为0.346、0.404、0.443;(3)A、B、C三个水泥池,连续培养59~70 d,经52-63次采收,分别收获褶皱臂尾轮虫1494×108、1748×108、2149.2×108个,合计收获轮虫5391.2×108个,饵料生产力为7.17、8.54、10.55;(4)采用合适的干酵母投喂方法和投喂量,定期带水采收,使轮虫密度保持在200~300个／ml,并补充等量培养用水,可以获得长期稳产的效果。 相似文献
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轮虫,是海产鱼类仔、稚鱼的初期饵料。而培养轮虫的方法主要有二种。一是饲用海水小球藻,二是投喂酵母类以进行高密度培养。但实践表明,用酵母类培养的轮虫投喂鱼类仔、稚鱼易造成仔、稚鱼的大量死亡。竟其 相似文献
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Commercial Spirulina, Chlorella, and a methanol-grown yeast were tested as sole food source for the rotifer Brachionus plicatilis. Some good results were obtained, but only with medium salinity (18%) and none with seawater (35‰). In comparison with a constant supply of food, different adjustments of the food level to the population density were tested: a proportional and continuous or stepwise adjustment and a less proportional one. The less than proportional adjustment was found to be more advisable for a continuous culture with a daily renewal of a quarter of the volume. The three single-cell proteins appeared to be suitable with 35–55 rotifers produced per ml per day. However, the food conversion was poor: in the best cases 4.2 g of food were required per 106 rotifers produced. In practice, two formulated diets were preferred for mass culture trials in 60-l tanks: the first (P 72) included 40% Spirulina, 40% yeast and different additives; the second was composed of 67% of diet P72 and 33% of the freeze-dried alga Platymonas suecica. The production was 44 and 35 rotifers, respectively per ml per day, with 3.6 and 2.9 g of food, respectively per 106 rotifers,produced. Freeze-dried algae did not appear to be necesary for rotifer production, but the nutritional quality of such rotifers should be investigated. 相似文献
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Abu Talib Ahmad Shigehisa Yamasam Hachiro Hirata 《Journal of the World Aquaculture Society》1991,22(4):230-234
The optimum feeding rate of the rotifer Bruchionus plicutilis was investigated to determine the best conditions for growth of the rotifer, and also in order to maintain good water quality of the culture. Fifty rotifers of a large size strain were cultured individually at five food density levels of Nannoehloropsis sp. (0.5 ± 106 , 1.5 ± 106 , 3 ± 106 , 5 ± 106 , and 8 ± 106 cells/ml). At each level, daily survival and offspring production were recorded until the death of the final individual. The data obtained were analyzed by the life table method. The maximum value of three growth indices (the net reproduction rate, mean life expectancy at age 0, and intrinsic rate of increase) was obtdned at the food density of 1.5 ± 106 cells/ml. The ration size was calculated to be 325% (dry weight)/ day, which is equivalent to about 70% of the saturated feeding rate. It was suggested that the feeding rate should be controlled to lower than the saturated value for an efficient mass culture. 相似文献
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Food ingestion,consumption and selectivity of pompano,Trachinotus ovatus (Linnaeus 1758) under different rotifer densities 
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下载免费PDF全文 Zhenhua Ma Huayang Guo Dianchang Zhang Chaoqun Hu Shigui Jiang 《Aquaculture Research》2015,46(11):2593-2603
The growth, survival, food selection and consumption of pompano larvae under different rotifer densities as well as their colour preference during the rotifer feeding stage were examined in this study. Growth and survival of fish larvae were not significantly affected when rotifer density was between 10 and 20 mL?1. Fish larvae grew slower at 1 and 40 rotifers mL?1 than at 10 and 20 rotifers mL?1, and higher fish survival was achieved when fish larvae were exposed to 10 and 20 rotifers mL?1. The rotifer density of 1 mL?1 not only reduced food ingestion during the early stage, but also delayed diet switch from rotifer to copepod nauplii. On 5 days post hatching (DPH), larval pompano ingested more rotifers in dark‐coloured tanks and ingested more rotifers when prey colour was green. Based on the results obtained in the present study, the culture of larval pompano larvae is recommended using dark wall tanks with a feeding density of 10–20 rotifers mL?1 during the initial feeding stage. This study proposes a management protocol to use appropriate type and quantity of live food to feed pompano larvae in a hatchery rearing condition, which could be applicable to the culture of fish larvae in other marine fish species. 相似文献
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Gamma‐aminobutyric acid (GABA) has been shown to enhance the reproduction of the rotifer Brachionus plicatilis Muller in stressful culture conditions. During the enrichment of rotifers for feeding to marine fish larvae, they are usually stressed as a result of exposure to different marine oils and high population densities. This typically results in decreased rotifer survival, reproduction and swimming activity. In the present study, we used GABA to increase rotifer reproduction and the swimming activity of rotifers in enrichment cultures. GABA treatment 24 h before high density enrichment enhanced reproduction during enrichment culture, but not when carried out simultaneously with enrichment. Swimming activity was not significantly affected by GABA treatment 24 h before or simultaneously with nutrient enrichment. 相似文献
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The culture quality of rotifers, Brachionus plicatilis, was compared for yeast and algal-based cultivation diets. The rotifer quality was evaluated based on factors which may affect survival and growth of marine fish larvae, including individual length and biomass, nutritional value, bacterial content, and rotifer viability. The diets used were monocultures of Tetraselmis sp., Isochrysis galbana, Pavlova lutheri, and baker's yeast plus capelin oil.Rotifers grown to early stationary phase with equal food rations exhibited equal length distribution, but the rotifer individual biomass was 10–25% higher for yeast-grown rotifers than for algal-grown rotifers.All diets gave equal protein content of individual rotifers. The lipid content was slightly higher and the ratio of protein to lipid was slightly lower with yeast plus capelin oil than with algae. The fatty acid composition of the rotifers was closely related to that of their dietary lipids and the 3 fatty acid content was not systematically different for the two types of diets.Use of yeast plus capelin oil resulted in considerably higher numbers of both suspended and rotifer-associated bacteria than with use of algal diets. No differences were found for the algal species involved. The viability of the rotifers in a temperature and salinity test was far better in groups fed algae than in groups fed yeast plus capelin oil.The results indicated an overall better quality of rotifers cultivated with algae than for rotifers cultivated with yeast and oil. Questions related to proteins and the microflora of rotifers should be further emphasized in future research. 相似文献
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Beatriz Reguera 《Aquaculture (Amsterdam, Netherlands)》1984,40(2):103-108
High levels of ciliates in mass cultures of the rotifer Brachionus plicatilis result in considerably reduced yield. This is caused by the activity of the ciliates which bring about aggregation of food algae and hence reduce their availability to the rotifers. Extracellular products of the ciliates do not seem to be involved. Ciliates thrive in Dunaliella tertiolecta cultures, both living and pasteurized, and with or without antibiotics.The ciliates compete with the rotifers, and their presence can become undesirable. some practical solutions to control or eradicate ciliates from rotifer cultures are suggested. 相似文献
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褶皱臂尾轮虫的敞池增殖 总被引：7，自引：0，他引：7
池塘的底泥中蕴藏着丰富的轮虫休眠卵,搅动底泥使其上浮可促进萌发、繁衍形成轮虫种群数量高峰期。高峰期的生物量和持续的时间与水温、食物、pH等诸多因子有关,其中饵料浮游植物的种、量是重要影响因子。在大型浮游植物长期占优势的水体,轮虫种群数量增长极慢。作者还根据轮虫的现存量估算了轮虫池的供饵能力。 相似文献
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The dietary value of dried, commercial Chlorella was compared with that of living marine Chlorella, and yeast, in relation to growth of the rotifer Brachionus plicatilis raised individually and by batch culture methods.A concentration of 50 μg/ml of dried Chlorella powder is near an optimal density for rotifer growth. The dried material in suspension is less effective for growth than living marine Chlorella, although it is much more effective than a suspension of yeast at the same density (50 μg/ml).In batch culture (12-l glass vessel), the rotifers grew from an initial inoculation of 13.2 individuals/ml to a density of 434 individuals/ml by the 16th day. About 107 rotifers could be removed from one batch culture in five harvests in the 41-day experimental period.The results indicate that dried Chlorella powder is an effective food for the rotifer Brachionus plicatilis. 相似文献
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The effect of different rotifer feeding regimes on the growth and survival of yellowtail kingfish Seriola lalandi (Valenciennes, 1833) larvae 下载免费PDF全文
下载免费PDF全文 First feeding success is critical to larval marine finfish and optimization of live feed densities is important for larval performance and the economics of commercial hatchery production. This study investigated various rotifer feeding regimes on the prey consumption, growth and survival of yellowtail kingfish Seriola lalandi larvae over the first 12 days post hatch (dph). The common practice of maintaining high densities of rotifers (10–30 ind. mL?1) in the rearing tank was compared to a low density feeding technique, where 5–8 ind. mL?1 of rotifers were offered. A ‘hybrid’ feeding regime offered rotifers at the high density treatment until 5 dph and the lower feeding densities thereafter. There was no significant difference in larval survival (hybrid: 28.9 ± 7%, low density: 17.3 ± 5% and high density: 17.2 ± 9%) or growth (hybrid: 6.12 ± 0.18 mm, low density: 6.03 ± 0.10 mm and high density: 6.11 ± 0.23 mm) between treatments. Rotifer ingestion was independent of rotifer density throughout the trial and increased with larval age, with larvae at 4 dph ingesting 22 ± 1.5 rotifers larvae?1 h?1 and by 11 dph ingesting 59 ± 1.6 rotifers larvae?1 h?1. These data demonstrate that from first feeding, yellowtail kingfish larvae are efficient at capturing prey at the densities presented here and consequently significant savings in rotifer production costs as well as other potential benefits such as facilitation of early weaning and improved rotifer nutritional value may be obtained by utilizing lower density rotifer feeding regimes. 相似文献
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Larvae of Japanese smelt Hypomesus nipponensis have unique salinity adaptability and a small gape size; consequently, no system has been developed to feed them for stable high growth and survival. Therefore, suitable conditions for larval growth of Japanese smelt remain to be studied. In an earlier study, we developed a Japanese smelt rearing method using a closed recirculating system. Using that system for this study, we examined recirculation rate effects on Japanese smelt larval growth because the recirculation rate can affect larval feeding opportunities through regulation of the rotifer retention time in the rearing tanks. Using 0.2–0.3% salinity and water temperatures of less than 20 °C, we fed Japanese smelt larvae with SS-type rotifer and commercial feed. Results indicated that larval growth with a recirculation rate of less than 648% per day (LR) is superior to that achieved at a rate of over 2160% per day (HR). Moreover, rotifer density under the LR condition declines more slowly than under the HR condition. These results demonstrated that the recirculation rate affects their growth, perhaps by affecting the opportunities for larvae to ingest the rotifers. Furthermore, SS-type rotifers are available for feeding teleosts in a wide range of water temperatures and salinities.
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