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1.
本试验从荆州周边多个猪场中采集腹泻仔猪粪便，进行细菌的分离培养，通过革兰氏染色、氧化酶试验、葡萄糖发酵试验鉴定为肠杆菌科，再通过吲哚试验、MR实验、VP实验、枸橼酸盐试验等生化试验鉴定为大肠杆菌。通过接种CAYE培养基制备LT及提取，再通过兔皮肤蓝斑试验、乳兔口服试验、兔回肠结扎试验等试验进行LT的鉴定。 相似文献
2.
文章旨在研究茯苓多糖对仔猪生长性能、养分表观消化率及免疫功能的影响。试验将120头三元杂交断奶仔猪随机分为4组，每组3个重复，每个重复10头。其中试验1组饲喂基础日粮，试验2、3、4组分别在基础日粮中添加0.05%、0.1%、0.2%的茯苓多糖，预试期7 d，试验期40 d。结果表明：（1）与试验1组相比，试验2、3、4组仔猪平均日增重、平均日采食量显著提升（P<0.05）；试验3、4组仔猪料重比显著降低（P<0.05）。（2）试验2、3、4组粗蛋白质表观消化率相较试验1组显著提升（P<0.05）；试验3、4组粗灰分表观消化率较试验1组显著提升（P<0.05）；试验2、3、4组磷表观消化率相较试验1组显著提升（P<0.05），同时试验3组磷表观消化率较试验2、4组显著提升（P<0.05）。（3）与试验1组相比，试验2、3、4组仔猪血清中IgA、IgM、IgG、IL-2含量显著升高（P<0.05），其中试验3组提升效果最佳。综上所述，添加不同水平茯苓多糖可以提高仔猪生长性能、养分表观率及免疫功能，但随着添加量的提升，其带来的正向效果反而会下降，在... 相似文献
3.
无菌采集家兔小肠病料，经过培养特性检查、生化试验（MR与VP、接触酶试验、吲哚试验、糖发酵试验、柠檬酸盐利用试验等）等病原学分离鉴定，确诊为致病性大肠杆菌病。根据血清学鉴定结果，共有1种血清型（O15）。药敏试验结果表明，该菌对丁胺卡那霉素、氧氟沙星、环丙沙星的敏感度最强，对氯霉素、新霉素、庆大霉素、恩诺沙星敏感度居中，青霉素抑菌作用最低。 相似文献
4.
试验旨在研究不同水平酯化植物甾醇对断奶仔猪生长性能、免疫功能及血清生化指标的影响。选取100头28日龄的断奶仔猪随机分成4组，每组5个重复，每个重复5头。对照组仔猪饲喂基础日粮，试验1组、试验2组、试验3组分别在基础日粮中添加200、400和800 mg/kg的酯化植物甾醇。预试期1周，正式试验期4周。结果表明，与对照组相比，试验2组仔猪末重显著提高（P<0.05），试验2组、试验3组仔猪的料重比显著降低（P<0.05），试验1组、试验2组、试验3组仔猪的腹泻率降低（P<0.05）。与对照组相比，试验2组和试验3组仔猪血清中免疫球蛋白A含量显著提高（P<0.05），试验2组免疫球蛋白G含量显著提高（P<0.05）；试验1组、试验2组、试验3组仔猪血清白细胞介素-10含量显著提高（P<0.05），肿瘤坏死因子-α含量显著降低（P<0.05）；试验2组和3组仔猪血清中胆固醇、甘油三酯含量显著降低（P<0.05），试验2组仔猪血清中高密度脂蛋白胆固醇含量显著提高（P<0.05），试验2组和3组仔猪血清中尿素氮含量显著降低（P<0.0... 相似文献
5.
对电力变压器进行高压试验是确保其安全运行的重要手段之一，本文结合工作实践，对电力变压器进行高压试验技术的试验条件、试验方法、安全措施、试验内容等进行深入探讨，并从三大方面提出提高电力变压器高压试验效率的措施，以供参考。 相似文献
6.
本试验旨在研究中草药添加剂对高温季节奶山羊产奶量和乳品质的影响。试验采用单因子随机区组试验设计，选择60只体重相近的关中奶山羊公羔，随机分成3组，每组20只。对照组饲粮为基础精料和青干草、试验Ⅰ组为基础精料和青干草+0.15 g/kg中草药干物质、试验Ⅱ组为基础精料和青干草+0.20 g/kg中草药干物质。试验羊只自由饮水，自由采食青干草。结果表明：随着中草药水平的增加，奶山羊的平均体温在数值上呈下降趋势，但是试验各组的平均体温显著不差异（P>0.05）。试验各组的干物质采食量变化不大，差异不显著（P>0.05）；试验Ⅱ组的产奶量比对照组、试验Ⅰ组分别高42.57%、40.67%，差异显著（P<0.05）；试验Ⅱ组的4%校正乳产量比对照组、试验Ⅰ组分别高40.13%、39.19%，差异显著（P<0.05）。随着中草药水平的增加，奶山羊乳脂率在数值上呈下降趋势，试验Ⅱ组的乳脂率比对照组、试验Ⅰ组分别低3.35%、2.34%，差异不显著（P>0.05）；试验Ⅱ组的乳蛋白率最高，且比对照组、试验Ⅰ组分别高5.62%、4.06%，差异不显著（P>0.05）；... 相似文献
7.
为了研究20-50kg生长猪甘露寡糖的适宜添加水平，本研究采用单因子对比试验，选用60日龄“杜长大”三元杂交猪，体重在20kg左右，每组10头，试验设对照组、试验1组、试验2组和试验3组，分别在基础日粮中添加：甘露寡糖（MOS）0mg/kg、160mg／kg、480mg／kg和800mg／kg。结果表明：①在饲粮中添加甘露寡糖，生长猪前期、后期及全程的日增重均比对照组高，并有所提高。在前期，试验2组（MOS480mg／kg）提高最大，提高趋势明显，与对照组（MOS0mg/kg）相比差异极显著（P〈0．01），与试验3组（MOS 800mg／kg）、试验1组（MOS160mg/kg）相比差异显著（P〈0．05）。而在后期，仅试验2组与对照组达到显著水平（P〈0．051；试验各组间差异不显著（P〉0．05），对日增重的提高强度有所下降。全程日增重分析，试验2组与对照组差异极显著（P〈O．01），与试验1组显著（P〈0．05）及与试验3组显著（P〈0．05）；但试验1组、试验3组、对照组间差异不显著（P〉0．05）。②试验各组料肉比均比对照组低，有降低趋势；从前期、后期及全程看，试验2组降低明显。③生物学综合评定值，无论前期、后期及全程，由高到低依次为：试验2组〉试验3组〉试验1组〉对照组。④在饲粮中添加MOS可提高生长猪的抗病力，降低腹泻率；在20-30kg前期较30～50kg后期明显，并以试验2组较好，试验3组有轻微诱发腹泻。 相似文献
8.
牛精液进行冷冻处理，利用不同初冻方法，对比精子活力效果。熏蒸时间分为6、8、10min3个试验组合，采用常规牛冻精稀释液配方。试验结果：熏蒸时间6、8、10min3个试验组精子活力分别为0．286土0．0353、0．351土0．0326和0．319±0．0307，其中试验2组最高，试验3组次之，试验1组最低。精子顶体完整率分别为37．83、46．36和42．62，其中试验2组高于试验1组，其它组间比较差异不明显。畸形率分别为29．62、24．02和26．58，试验2组和试验3组间差异不显著，两组与试验1组相比差异均显著。试验表明，在熏蒸距离为2cm时，熏蒸时间控制在8min，其精液解冻后精子活力符合牛输精要求的国家标准。 相似文献
9.
试验研究不同水平的发酵饲料替代基础日粮对肉鸭生长性能、免疫力及血清生化指标的影响。试验选择1日龄、健康的樱桃谷肉鸭800只，随机分为4组，每组5个重复，每个重复40只。对照组、试验1组、试验2组、试验3组分别使用0、2.5%、5.0%、10.0%的发酵饲料替代基础日粮。试验期42 d。结果显示，1～21日龄，试验2组和试验3组肉鸭21日龄的体重显著高于对照组（P<0.05），料重比显著低于对照组和试验1组（P<0.05）。22～42日龄，试验2组和试验3组肉鸭42日龄的体重、平均日增重均显著高于对照组和试验1组（P<0.05）。1～42日龄，试验2组和试验3组肉鸭平均日增重均显著高于对照组和试验1组（P<0.05），料重比显著低于对照组和试验1组（P<0.05）。试验2组和试验3组肉鸭血清中的免疫球蛋白A (IgA)、干扰素-γ (IFN-γ)含量显著高于对照组（P<0.05），各试验组肉鸭血清免疫球蛋白G (IgG)、白细胞介素-2 (IL-2)含量显著高于对照组（P<0.05）。试验2组和试验3组肉鸭血清中总蛋白（TP）和白蛋白（ALB）含... 相似文献
10.
文章旨在对比紫花苜蓿与青贮玉米对肉牛的饲喂效果。试验选取30头健康状况良好，体重为330 kg的肉牛，随机分为5组，每组6头。5组肉牛均采用相同精饲料饲喂，各组肉牛的粗饲料组成如下：100%青贮玉米（对照组）、100%紫花苜蓿（试验1组）、75%紫花苜蓿+25%青贮玉米（试验2组）、50%紫花苜蓿+50%青贮玉米（试验3组）、25%紫花苜蓿+75%青贮玉米（试验4组），试验为期180 d。结果表明，（1）试验2组肉牛末重、平均日增重显著高于对照组、试验4组（P<0.05），试验1、2组肉牛平均日采食量显著高于试验3、4组（P<0.05），试验2、3组肉牛料重比显著低于对照组、试验4组（P<0.05）。（2）试验1、2、3组胴体重显著高于对照组（P<0.05），试验3组净肉重显著高于对照组、试验4组（P<0.05），试验3组屠宰率、净肉率、胴体产肉率、眼肌面积数值最高，但差异不显著（P>0.05）。（3）试验1组红度a显著高于对照组（P<0.05）,4组其他肉品质指标差异不显著（P>0.05）。说明75%紫花苜蓿+25%青贮玉米组合粗饲料能... 相似文献
11.
Ozdemir U Loria GR Godinho KS Samson R Rowan TG Churchward C Ayling RD Nicholas RA 《Tropical animal health and production》2006,38(7-8):533-540
The efficacy of danofloxacin (Advocin A180) was evaluated for the treatment of contagious caprine pleuropneumonia (CCPP) caused by Mycoplasma capricolum subsp. capripneumoniae. Ten healthy Angora goats, confirmed free of CCPP, were exposed to clinically affected animals from a natural outbreak in Thrace, Turkey. After 14 days exposure, 8 goats showed pyrexia ( > or = 41 degrees C). Shortly after, the Angora goats were divided randomly into two groups. Five of these were injected with danofloxacin (6 mg/kg subcutaneously), which was repeated after 48 h; the five remaining animals received saline. Goats were monitored clinically and blood samples were collected for serology. Animals with severe disease were withdrawn from the trial. Goats completing the study were euthanized at day 42. Lung tissue and bronchial fluid were collected for mycoplasma isolation. All danofloxacin-treated goats showed resolution of clinical disease by the end of the trial. Two saline-treated goats failed to complete the study owing to CCPP. Danofloxacin-treated goats showed fewer lung lesions and had significantly lower combined clinical scores than saline controls (p < 0.001). Danofloxacin was found to be highly effective in the treatment of CCPP in goats. 相似文献
12.
羊传染性胸膜肺炎又称羊支原体性肺炎,由支原体所引起的一种高度接触性传染病,其临床特征为高热,咳嗽,胸和胸膜发生浆液性和纤维素性炎症,主要危害1～3月龄的羔羊,常呈急性和慢性经过,病死率较高,是绵羊养殖中较为常见的呼吸道疾病。临床上大多采用抗生素进行治疗,但常常发生耐药情况而导致疗效差的情况。近年来,我们采用早期应用中草药辅以较为敏感的抗生素进行治疗,取得了良好的治疗效果。本文就对常用的4组中药组方开展疗效观察,筛选出了一组疗效最佳的中药组方,以期为临床治疗提供解决方案。 相似文献
13.
绵羊肺炎支原体的培养和PCR鉴定 《畜牧与饲料科学》2016,37(11):97-97
安徽省3个山羊场有羊只发生肺炎症状,用支原体培养基对病料进行病原分离,经传代和纯化后分离到3株支原体,随后对分离到的支原体进行了形态学观察、PCR鉴定和序列比对,结果显示分离到的支原体均为绵羊肺炎支原体。 相似文献
14.
Isolation of Mycoplasma mycoides subspecies mycoides from Polyarthritis and Mastitis of Goats in Canada 总被引：3，自引：0，他引：3
Ruhnke HL Rosendal S Goltz J Blackwell TE 《The Canadian veterinary journal. La revue veterinaire canadienne》1983,24(2):54-56
The clinical signs, pathomorphological changes, and microbiological findings in Canadian goats infected with Mycoplasma mycoides subspecies mycoides are discussed. The disease affected mainly young goats and was characterized by septicemia and polyarthritis. Mastitis followed by septicemia was seen in two mature goats. The diagnosis was made by culture and identification of the mycoplasma. Infected goats without clinical signs were identified by cultural and serological (complement fixation) techniques. Healthy carriers are presumably able to transmit the infection and may have brought the disease to Canada. 相似文献
15.
16.
Pleuropneumonia was induced in goats by inoculation of mycoplasma strain ... SGP1 (F38 type) and spread readily from them to in-contact goats which in turn passed it to other in-contact goats. This emphasises the similarity between the experimentally produced disease and classical contagious caprine pleuropneumonia (CCPP) thus supporting the view that SGP1 has an aetiological role in CCPP in the field. 相似文献
17.
Mycoplasma mycoides subsp. capri associated with goat respiratory disease and high flock mortality 总被引：5，自引：0，他引：5
Hernandez L Lopez J St-Jacques M Ontiveros L Acosta J Handel K 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(4):366-369
A high mortality outbreak of respiratory mycoplasmosis occurred in goats in Mexico. The clinicopathologic presentation resembled contagious caprine pleuropneumonia caused by Mycoplasma capricolum subspecies capripneumoniae. By using a battery of polymerase chain reaction assays, the mycoplasma associated with this outbreak was identified as Mycoplasma mycoides subsp. capri. 相似文献
18.
Three groups of 15 goats each were immunised against contagious caprine pleuropneumonia (CCPP) using sonicated antigens of the F-38 strain of mycoplasma incorporated in incomplete Freund's adjuvant (IFA), emulsified in aluminium hydroxide and phosphate buffered saline respectively. Three months after immunisation, five goats from each group were challenged by the in-contact method. The goats immunised with the antigen incorporated in IFA were all solidly immune to the challenge whereas only two of five of the goats in the other two groups were protected. When the remaining 10 animals from each group were challenged six months after immunisation, those immunised with the antigen in IFA were still solidly immune while only two goats from each of the other two groups were protected. These results show that effective immunity against CCPP caused by the F-38 strain can be induced by vaccination with sonicated F-38 antigens emulsified in IFA. 相似文献
19.
Influence of induced disease states on the disposition kinetics of imidocarb in goats 总被引：2，自引：0，他引：2
The influence of fever, induced by different agents, on the disposition kinetics of imidocarb was determined in goats. Escherichia coli endotoxin (0.2 microgram/kg), Trypanosoma evansi (10(7) in 1 ml sterile glucose citrate), and Infectious Bovine Rhinotracheitis virus (10(6.5)TCID50) were the agents administered to induce the febrile state. In control and febrile animals the two-compartment model was used to describe the disposition kinetics of the drug. Fever caused significant changes to occur in the apparent volume of distribution and the body (systemic) clearance of imidocarb, but the half-life remained unchanged. The statistical significance of the changes in these pharmacokinetic parameters varied with the etiology of the febrile state. E. coli endotoxin and IBR virus caused corresponding decreases in apparent volume of distribution and clearance of imidocarb, while fever induced with T. evansi caused highly significant increases in both pharmacokinetic parameters. It was concluded that the alterations in the disposition kinetics of imidocarb that occurred in the febrile goats were related not only to the febrile reaction per se but also to the pathophysiology of the disease condition. 相似文献
20.
S Rosendal 《American journal of veterinary research》1984,45(11):2396-2402
The caprine variant of Mycoplasma mycoides subsp mycoides causes septicemia with coagulopathy in goats. Pathogenetic mechanisms that might explain the coagulopathy, the ability of the Mycoplasma to persist in the blood, and its specificity for goats were studied. Severe endothelial damage was seen by electron microscopy of goat aorta tissue exposed in vitro to 10(7) colony-forming units of mycoplasmas. The Mycoplasma did not damage 51Cr-labeled adherent cells from peripheral blood of goats. The hemolytic complement titer was reduced by 94%, 50%, 50%, and 25% in guinea pig, calf, sheep, and goat serum, respectively, 30 minutes after treatment with 8 X 10(9) colony-forming units of the Mycoplasma. Freshly prepared serum from these animal species killed the Mycoplasma. Heat-inactivated serum was not mycoplasmacidal. Complement from these 4 animal species was activated by the Mycoplasma through the classical pathway, because ethyleneglycoltetraacetic acid precipitation of serum Ca2+ inhibited activation. Proof that the classical pathway was functional in goats was not conclusive because Ca2+ supplementation of ethyleneglycoltetraacetic acid-treated serum did not restore complement activity. Endothelial damage and complement activation may explain the coagulopathy. The function that complement activation may have in the inflammatory response of this disease is not known. Difference in susceptibility of calves, sheep, and goats to M mycoides septicemia cannot be explained by species variation in complement mycoplasmacidal activity. 相似文献