共查询到17条相似文献，搜索用时 171 毫秒
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应用微卫星PCR对中国3个品系实验用小型猪的遗传检测 总被引：6，自引：0，他引：6
以 12个 STR(shord tandem repeat)座位的 PCR对中国 3个品系实验用小型猪进行了遗传检测。结果 ,3个座位品系间扩增结果相同 ;9个座位呈现品系间多态性 ,其中 2个座位在 3个品系间均具有差异。贵州小型香猪与西双版纳近交系小耳猪间有 6个座位有差异 ,其遗传距离为 0 .186 4;贵州小型香猪与广西巴马小型猪间有 3个座位有差异 ,其遗传距离为 0 .12 73;广西巴马小型猪与西双版纳近交系小耳猪间有 5个座位有差异 ,其遗传距离为 0 .1333。由此表明 ,3个品系小型猪间的遗传背景具有一定差异 ,同时表明这 9个座位可选择为小型猪遗传检测的多态性座位。 相似文献
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小型猪作为人类疾病动物模型在生物医学研究中的应用 总被引：1，自引：0，他引：1
与啮齿类小型实验动物模型相比,小型猪因其解剖学、生理学、疾病发生等方面与人类极为相似,且价廉、无伦理问题等方面的原因,在生物医学研究领域中具有广阔的应用前景,尤其在人类疾病动物模型制作、新药安全性评价、异种器官移植等领域应用更广.论文主要综述了国内外小型猪作为心血管疾病、消化系统疾病、皮肤烧(烫)伤、口腔疾病、异种器官... 相似文献
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小型猪,又称minipig,具有适应性强、耐粗饲、繁殖效率高、抗逆性强等特点;与啮齿类小型实验动物模型相比,小型猪因其解剖学、生理学、发病机理等方面与人类极为相似、价格低廉,无伦理问题等方面的原因,小型猪作为医学研究中常用的实验动物不仅受到人们的关重,而且具有广阔的应用前景,尤其在制作人类疾病动物模型、评价新药安全性、... 相似文献
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应用RAPD技术对中国3品系实验用小型猪进行亲缘关系的分析 总被引：11，自引：0，他引：11
随机扩增多态ＤＮＡ (ＲａｎｄｏｍＡｍｐｌｉｆｉｅｄｐｏｌｙｍｏｒｐｈｉｃＤＮＡ ,ＲＡＰＤ)技术 ,在动物、植物、微生物种属鉴定 ,亲缘关系的划分及多态性分析中得到广泛应用 ,但应用于小型猪方面的研究报道很少 ,尤其是对我国特有的实验用小型猪的研究 ,目前国内外尚未见报道。贵州小型香猪、广西巴马小型猪及西双版纳近交系小耳猪由于其近交程度高、遗传性稳定以及良好的实验应用效果 ,已引起了国内外相关领域的关注。本实验应用 1 0 0条随机引物对上述 3品系小型猪的基因组ＤＮＡ进行ＲＡＰＤ研究 ,旨在筛选具有品系间多态性的ＲＡ… 相似文献
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设计引物通过RT-PCR扩增巴马香猪和贵州小型猪香猪药物代谢关键P450基因CYP3A29基因的全长编码区,纯化并克隆入pMD 18-T载体,通过序列测定和比对分析其序列变异情况。17头小型猪共发现CYP3A29基因的5个单核苷酸变异位点,均表现为同义替换,对编码的氨基酸序列无影响;猪CYP3A29的单核苷酸变异和品系相关,在不同品系中可表现出品系特有的单核苷酸变异;贵州小型香猪G3 a2902发现1个可变剪接,缺失89 bp并产生移码,改变编码蛋白。本研究克隆的巴马香猪和贵州小型香猪CYP3A29基因及其序列变异情况,可为其作为药物代谢模型的应用提供技术资料。 相似文献
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巴马小型猪动物模型在医学领域的研究进展 总被引：1，自引：0，他引：1
巴马小型猪是中国原产地闭锁繁殖的优质品种,具有遗传稳定性、理想的毛色分布、体型小、多产性、饲养要求低、抗病力强等优势,且在生理学、解剖结构、药物代谢、生化指标和疾病发生机制等方面与人类相似,已用于心血管、皮肤整形、内分泌及代谢、消化、口腔医学、异种移植、中医、麻醉学、神经系统等广泛医学领域的研究,建立了心肌缺血、冠脉微栓塞、卵圆孔未闭、动脉粥样硬化、皮肤创伤、经皮给药、代谢综合征、糖尿病及其并发症、梗阻性黄疸、慢性胰腺炎、肝硬化、肝脏射频消融、牙周组织炎症、下颌骨缺损、异种器官/细胞移植、中医征候、手术麻醉、脑缺血、支架植入、休克等一系列类似于人类疾病的动物模型,表明巴马小型猪作为理想的试验动物在生物医学研究中具有广阔的应用前景。作者综述了巴马小型猪作为试验动物在各医学领域的应用进展,了解现有巴马小型猪疾病模型的种类、构建方法及病理特征,有助于进一步探索具体的病因、发病机制、并发症和防制手段等,为拓展研究范围、提高医学水平奠定了科学基础。 相似文献
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荣昌猪是我国优良地方品种,巴马香猪和贵州小型香猪为我国独特小型猪品系。本研究采用美国猪基因组协作计划推荐的19个微卫星标记对荣昌猪、荣昌猪B系、巴马香猪、贵州小型香猪及外来猪种大白猪进行了遗传学检测和分析。结果表明:19个微卫星位点在群体中均表现为多态,每位点等位基因数10~23个。5个猪种中荣昌猪B系的遗传多样性最高,荣昌猪次之;2种小型猪的遗传多样性较低,其中巴马香猪的等位基因数(121个)略大于贵州小型香猪(114个),但其遗传多样性指数(平均期望杂合度0.6535±0.0347)小于贵州小型香猪(0.6919±0.0227),反映了巴马香猪较低的基因杂合度和较小的遗传变异;大白猪的遗传多样性最低。从品系的共有等位基因来看,荣昌猪B系基因背景组成中其亲本品系荣昌猪所占比例(30.22%)要大于大白猪(24.37%);大白猪和其他4个猪群体的共享等位基因数均较低(21.24%~25.12%);巴马香猪和贵州小型香猪之间共有等位基因数最高(45.06%)。采用基于遗传距离的NJ法和基于基因频率的最大似然法进行系统聚类,除了大白猪明显为独立分支及荣昌猪B系表现出较远的聚类关系外,其他3个猪种间的聚类有所不同。 相似文献
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Bama miniature pigs, which are local high-quality species of China, have the advantages of genetic stability, ideal hair distribution, microsomatia, prolificacy, low fodder demand, strong disease resistance, and they are similar to human beings in aspects of physiology, anatomical structure, drug metabolism, biochemical indicators, disease mechanism, etc. Bama miniature pigs have been used in the wide fields of medical research, such as cardiovascular system, skin plastic, endocrine and metabolism, digestive system, oral medicine, heterograft, Chinese medicine, anesthesiology, nervous system, etc., and been applied to establish the animal models which are similar to human diseases, such as myocardial ischemia, coronary micro-embolization, atrial septal defect, atherosclerosis, skin injury, transdermal drug, metabolic syndrome, diabetes mellitus and complications, obstructive jaundice, chronic pancreatitis, liver cirrhosis, liver radiofrequency ablation, periodontal tissue inflammation, mandibular defect, heterogeneous organ/cell transplantation, syndrome of Chinese medicine, anesthesia, cerebral ischemia, stent implantation, shock, etc. Bama miniature pigs, as an ideal experimental animal, have broad application prospects in biomedical research.In this article,we mainly summarized the progress of Bama miniature pigs in the field of medicine, to understand the types, construction methods and pathological characteristics of Bama miniature pigs disease models, which contribute to further explore the specific etiology, pathogenesis, complications, prevention and control measures, etc., and laid the scientific foundation for expanding the research scope and improving the medical level. 相似文献
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对“猪为六畜之首”及“以私养为主”的再认识 总被引：1，自引：0，他引：1
对“猪为六畜之首”及“以私养为主”的再认识张子仪，张仲葛我国畜牧业生产中“猪为六畜之首”。党和政府５０＊年代提出的发展养猪政策是：“以私养为主、公私并举”。历史地看，今天仍然符合国情。１９９３年全国养猪存栏约为３．９３亿头；出栏３．７８亿头，总产猪肉... 相似文献
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Experimental Isospora suis infections in miniature swine 总被引：2，自引：0，他引：2
Clinical responses to experimental Isospora suis infections were compared in Sinclair miniature pigs and cross-bred conventional pigs. Pre-patent periods, fecal consistencies, oocyst excretion dynamics, trends in surviving pig weights and lesions were similar in infected miniature and conventional pigs. The results indicate that the susceptibility of miniature pigs to I. suis is similar to that of conventional pigs. These findings should encourage their use as models for the study of neonatal coccidiosis. 相似文献
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小型猪特异性麻醉颉颃剂对小型猪肝肾功能的影响 总被引：1，自引：0，他引：1
为研究小型猪特异性麻醉颉颃剂对小型猪肝肾功能的影响,以复合麻醉剂(XFM)麻醉的中国试验用小型猪为试验模型,采用小型猪特异性麻醉颉颃剂进行催醒,检测催醒过程中血清谷草转氨酶(GOT)、谷丙转氨酶(GPT)、碱性磷酸酶(ALP)、尿素氮(BUN)、肌酐(CREA)的变化。结果发现:不同时间点小型猪血清中各项指标与麻醉前相比,差异不显著(P>0.05),均在生理范围内波动。表明临床剂量的XFM特异性麻醉颉颃剂对小型猪肝肾功能无明显影响。 相似文献
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Albuquerque C Morinha F Requicha J Martins T Dias I Guedes-Pinto H Bastos E Viegas C 《Veterinary journal (London, England : 1997)》2012,191(3):299-305
Periodontal disease (PD) refers to a group of inflammatory diseases caused by bacterial plaque in the periodontium and ranges from an early stage (gingivitis) to an advanced stage (periodontitis). It is a multifactorial disease that results from the interaction of the host defence mechanisms with the plaque microorganisms. Early detection, diagnosis and treatment are essential in the control of this disease. PD has an enormous impact on human and veterinary medicine due to its high prevalence. The most common animal PD models use dogs and non-human primates, although other animals (rats, mice, hamsters, rabbits, miniature pigs, ferrets, and sheep) have also been employed. Dog models have contributed significantly to the current understanding of periodontology. The most important clinical aspects of canine PD are considered in this review and the various animal models are examined with an emphasis on the role of the dog as the most useful approach for understanding human PD and in the development of new therapeutic and preventive measures. 相似文献
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Production of transgenic and non-transgenic clones in miniature pigs by somatic cell nuclear transfer 总被引：1，自引：0，他引：1
Kurome M Ishikawa T Tomii R Ueno S Shimada A Yazawa H Nagashima H 《The Journal of reproduction and development》2008,54(3):156-163
Miniature pigs have been recognized as valuable experimental animals in various fields such as medical and pharmaceutical research. However, the amount of information on somatic cell cloning in miniature pigs, as well as genetically modified miniature pigs, is much less than that available for common domestic pigs. The objective of the present study was to establish an efficient technique of cloning miniature pigs by somatic cell nuclear transfer. A high pregnancy rate was achieved following transfer of parthenogenetic (3/3) and cloned (5/6) embryos using female miniature pigs in the early pregnancy period as recipients after estrus synchronization with prostaglandin F2 alpha analog and gonadotrophins. The production efficiency of the cloned miniature pigs using male and female fetal fibroblasts as nucleus donors was 0.9% (2/215 and 3/331, respectively). Cloned miniature pigs were also produced efficiently (7.8%, 5/64) by transferring reconstructed embryos into the uteri of common domestic pigs. When donor cells transfected with the green fluorescent protein (GFP) gene were used in nuclear transfer, the production efficiency of the reconstructed embryos and rate of blastocyst development were comparable to those obtained by non-transfected cells. When transfected cell-derived reconstructed embryos were transferred to three common domestic pig recipients, all became pregnant, and a total of ten transgenic cloned miniature pigs were obtained (piglet production efficiency: 2.7%, 10/365). Hence, we were able to establish a practical system for producing cloned and transgenic-cloned miniature pigs with a syngeneic background. 相似文献
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Jeeyoung Kim Huijeong Ahn Heung-Myong Woo Eunsong Lee Geun-Shik Lee 《Veterinary research communications》2014,38(3):193-200
Inflammasomes, which are intracellular sensors of endogenous or exogenous danger signals, activate caspase-1, resulting in interleukin (IL)-1β maturation. Although most studies on inflammasomes have been performed in human and/or mouse-derived macrophages, porcine inflammasome activation has not been elucidated even though pigs are considered one of the best animal models for translational and preclinical investigations. In this study, we optimized detection of porcine IL-1β secretion, which is the most well established indicator of inflammasome activation, and compared inflammasome activation between miniature and domestic pigs as well as between porcine and murine macrophages. In our results, anti-sera against murine IL-1β had higher affinity to porcine IL-1β than anti-sera against human IL-1β, even though the amino acid sequence of porcine IL-1β was more similar to that of human IL-1β. In addition, there was no significant difference in inflammasome activation between miniature and domestic pigs. Furthermore, well established inflammasome triggers (ATP, nigericin, and crystals) in humans and mice had similar effects on porcine NLRP3 inflammasome activation. We further elucidated the upstream signaling pathway of porcine inflammasome activation using pharmacological inhibitors. Similar to the mechanisms of inflammasome activation in humans and mice, potassium efflux and reactive oxygen species generation were confirmed as key pathways in porcine inflammasome activation. Thus, inflammasome activation in pigs is not different from that in humans or mice. 相似文献