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1.
Phytase addition to swine diets has generally resulted in a marked increase in phosphorus (P) digestibility and in a reduction of faecal excretion of P. The number of phytases present in the market is growing and the origins of the microbial genes are becoming more diversified. The aim of this study was to compare the effects of 3 different phytases on P and calcium (Ca) digestibility in pigs. The control diet was based on soybean meal, maize and barley. The inclusion levels were 250 (Q1) and 500 U/kg (Q2) for an E. coli phytase (Quantum), 500 U/kg (Nat) for A. niger (Natuphos) and 750 U/kg (Ron) for P. lycii (Ronozyme P). All phytases significantly reduced faecal concentration of P. For Q2 this reduction was significantly higher than for Q1. P digestibility was improved by 13.8, 18.6, 18.3 and 17.9 percentage units by Q1, Q2, Nat and Ron respectively. The P equivalencies, considered as supplemental P digested compared to the non-supplemented diet of Q1, Q2, Nat and Ron were 0.492, 0.732, 0.678 and 0.678 g of available P/kg of feed respectively. Ca digestibility was also significantly improved by the phytases. It can be concluded that the 3 phytase preparations improved the digestibility and the apparent absorption of P and Ca in the growing pig fed a diet containing P exclusively from plant origin. The effect of including 500 U/kg of E. coli phytase on P digestibility was similar to those induced by the A. niger and P. lycii phytases at their recommended levels of 500 and 750 U/kg, respectively.  相似文献   

2.
High moisture corn (HMC) can undergo fermentation during storage that may improve its nutritional value for pigs. Stored HMC used in this study contained (n = 4) 75% dry matter (DM), and, on a DM basis, 63.8% starch, 0.5% mono and disaccharides, 9.9% protein, 19 mM acetic acid, 18 mM lactic acid (LA), 1.25 × 107 cfu g− 1 of LA producing bacteria, 2.88 g kg− 1 total phosphorus (P), and 1.26 g kg− 1 soluble P. The soluble P content was higher than in freshly harvested HMC (0.27 g kg− 1 DM) and indicates P release during storage. Studies were aimed at altering the nutritional value of stored HMC by controlled fermentation or steeping. Changes in LA content were not affected (P > 0.05) by addition of exogenous Lactobacillus and Bacillus bacteria. Steeping of HMC with phytase (Ronozyme) was examined at four inclusion levels (0, 500, 750, 1000 FTU kg− 1) and at either 21 °C or 37 °C. At 37 °C added phytase released virtually all phytate P within 6 h irrespective of the level (levels increased by 1.44 g P kg− 1 DM); at 21 °C, the increase was maximized at 1.2 g P kg− 1 DM after 24 h. The feeding value of HMC for pigs can be altered by steeping with exogenous phytase and does not appear to require microbial inoculants.  相似文献   

3.
Two maize based diets containing 2.3 g phytic P and 38 mg Zn/kg (P+) or 1.3 g phytic P and 25 mg Zn/kg (P−) were formulated. A third diet (P+/Phytase) was P+ supplemented with 3-phytase (500 FTU/kg). Each of these three basal diets, supplemented or not with 15 mg Zn/kg, was given during 20 days to weaned piglets and to 1-day-old chicks. Chicks fed the P− diets were removed from the data set because of an abnormally low feed intake.

In piglets, added Zn increased bone (P < 0.05) and plasma Zn (P < 0.01) irrespective of the basal diet (basal diet × Zn, P > 0.05). Supplementing P+ with phytase was more efficient in improving these indicators of Zn status than replacing P+ by P−. In chicks, supplemental Zn improved Zn status in a higher extent when added to P+ than to P+/Phytase (basal diet × Zn, P < 0.05). Phytase increased Zn status in a lesser extent than 15 ppm supplemental Zn. More Zn was soluble in gizzard than in stomach which corresponded to the differences in pH (4.2 vs 5.0). Phytase increased soluble Zn in stomach (P < 0.05) but not in gizzard. These results suggest a higher availability of Zn in chicks than in piglets in the absence of phytase. This may explain the higher efficacy of phytase for improved Zn availability in piglets than in chicks.  相似文献   


4.
Four trials investigated the effect of high levels of three phytase enzymes on P and protein utilization in chicks. The three phytases were derived from Aspergillus (Fungal Phytase 1), Peniophora (Fungal Phytase 2), and E. coli. Within each assay, 8-d-old male chicks were given ad libitum access to their experimental diet for 10 to 14 d. For Trials 1, 2, and 3, the basal diet was a corn-soybean meal diet deficient in P that was analyzed to contain 23% CP and 0.38% total P (0.10% estimated available P, as-fed basis). Phytase supplementation levels were based on the assessment of phytase premix activity (i.e., P release from Na phytate at pH 5.5 and 37 degrees C). In Trial 1, supplementation of inorganic P from KH2PO4 (0 to 0.20%) resulted in a quadratic (P < 0.05) response in weight gain, gain:feed, and tibia ash concentration but a linear (P < 0.01) increase in tibia ash weight. Tibia ash was higher (P < 0.01) for chicks fed E. coli phytase than for those fed Fungal Phytase 1 at 500, 1,000, and 5,000 phytase units (FTU)/kg, but did not differ between these two phytases at 10,000 FTU/kg. In Trial 2, E. coli phytase supplementation at 1,000 FTU/kg maximized growth and bone responses, whereas addition of either of the two fungal phytases resulted in increasing responses up to 5,000 and 10,000 FTU/kg. Dietary addition of Fungal Phytase 2 resulted in the poorest (P < 0.01) responses among the three phytases. Escherichia coli phytase supplementation at 10,000 FTU/kg in Trial 3 resulted in tibia ash (millligrams) responses that were greater (P < 0.05) than those resulting from either 0.35% inorganic P supplementation or 10,000 FTU/kg of Fungal Phytase 1 or 2. Trial 4 showed that E. coli phytase supplementation at either 500 or 10,000 FTU/ kg did not improve protein efficiency ratio (gain per unit of protein intake) of chicks fed low-protein soybean meal or corn gluten meal diets that were first-limiting in either methionine or lysine, respectively. These results demonstrate that high dietary levels of efficacious phytase enzymes can release most of the P from phytate, but they do not improve protein utilization.  相似文献   

5.
The effect of phytase and xylanase supplementation of a wheat-based pig diet on the ileal and total tract apparent digestibility of dietary components and minerals were studied in eight growing pigs fitted with a PVTC cannula in a randomized block design experiment. The diets (A and B) were similar in major ingredient composition and in nutrient content. In diet A, part of the limestone was replaced with di-calcium phosphate to increase the content of available phosphorus (P). Diet B was fed without or with supplementation with phytase (500 FTU/kg; diet BP), xylanase (4000 XU/kg; diet BX) and phytase + xylanase (500 FTU and 4000 XU/kg; diet BPX). There were no differences (P > 0.05) between diets in the ileal or total tract digestibility of organic matter (OM), NDF and crude protein (CP). The ileal and total tract digestibility for P and Ca differed (P < 0.05) between diets, while there were no treatment effects for Zn. The ileal and total tract digestibility for P and Ca was higher (P < 0.05) on diets BP and BPX than on the other diets. In conclusion, phytase improved the utilization of dietary P and Ca in a wheat-based diet, while xylanase had no additional benefits in terms of OM and CP digestibility or mineral utilization. Phytase had no effect on the digestibility of OM, CP or NDF.  相似文献   

6.
Phytate is an antinutrient in animal feeds, reducing the availability and increasing the excretion of nutrients. Phytases are widely used to mitigate the negative influences of phytate. This trial was designed to compare the efficacy of 2 Escherichia coli-derived phytases on broiler performance and bone ash as influenced by dietary phytate level. A total of 1,024 Arbor Acres male broilers were used with 8 replicate pens of 16 birds/pen. Experimental diets were based on low available phosphorus (avP; 1.8 g/kg) with low (6.40 g/kg) or high (10.65 g/kg) phytate. The low-avP diets were then supplemented with mono-dicalcium phosphate to increase the avP level to 4.5 g/kg, 500 phytase units/kg of phytase A, or 500 phytase units/kg of phytase B to create 8 experimental diets. Feed intake, BW gain, FCR, and livability were influenced by a P source × phytase interaction. Feed intake, BW gain, and livability were reduced and FCR was higher in broilers fed low-avP diets, particularly in the presence of high phytate. Phytase A or phytase B improved feed intake, BW gain, and FCR, particularly in the high-phytate diet. However, broilers fed phytase A ate more and were heavier than broilers fed phytase B. Tibia ash was lowest in broilers fed the low-avP diet and highest in broilers fed the diet supplemented with mono-dicalcium phosphate. Phytase increased tibia ash, and broilers fed phytase A had an increase in tibia ash compared with broilers fed phytase B. In conclusion, high dietary phytate reduced broiler performance. Phytase A and phytase B improved bone ash and growth performance, especially in the high-phytate diets. However, phytase A was more efficacious than phytase B, regardless of the level of phytate.  相似文献   

7.
Twenty four Duroc × Landrace male piglets, aged 21 days, were assigned to 1 of 4 experimental diets. Diets 1 and 2 contained 150 g kg− 1 wheat bran and diets 3 and 4 contained 90 g kg− 1 maize cobs as the major fibre source. All diets contained 480 g kg− 1 wheat and 200 g kg− 1 soybean meal. Diets 2 and 4 were supplemented with the following enzyme complex: 800 U/kg cellulase, 1800 U/kg glucanase and 2600 U/kg xylanase.

The replacement of wheat bran by maize cobs increased the acetic (P < 0.05) and decreased the butyric acid production (P < 0.05) in the cecum. Piglets fed diets with maize cobs had lower (P < 0.05) levels of butyric acid in the colon than those fed wheat bran.

The xylanolytic, pectinolytic and cellulolytic enzyme activities were higher (P < 0.05) in the cecum and colon of piglets fed the wheat bran based diets. The supplementation of the diet with the enzyme complex did not significantly affect the levels of short chain fatty acids formed in the small intestine and there was a non significant increase of the levels of acetic, propionic and butyric acids in the cecum and colon of piglets (P < 0.10). No interactions were present between fibre sources and enzyme addition.

The results suggest that the enzyme supplementation of the diet did not bring significant benefits to the animals and that when maize cobs replaces wheat bran in diets it negatively affects butyric acid production and fibre-degrading enzyme activity in the hindgut of piglets.  相似文献   


8.
An experiment was conducted with broilers from 22 to 33 days of age to evaluate the efficiency of six microbial phytases supplemented in diets (1500 FTU/kg) that were formulated with three different calcium:available phosphorus (Ca:Pavail) ratios (4.5:1.0, 6.0:1.0 and 7.5:1.0). A positive control diet without phytase was formulated with a Ca:Pavail ratio of 7.5:3.4 to meet the nutritional requirements of the broilers. The P and ash contents of the tibia, magnesium in the plasma, performance, balance and retention of phytate phosphorus (Pphyt), intake of total P and nitrogen (N), nitrogen‐corrected apparent metabolizable energy and apparent digestibility of dry matter of the diets were not influenced (p > 0.05) by the type of phytase or the dietary Ca:Pavail ratio. However, there was an interaction (p < 0.05) between the phytase type and the Ca:Pavail ratio for the retention coefficients of total P, Ca and N. Phytase B resulted in the highest Ca deposition in the tibia (p < 0.01). Phytases D, E and F reduced the Ca concentrations in the tibia (p < 0.01) and plasma (p < 0.05). Phytase D increased the P level in the plasma and decreased the total P excretion (p < 0.01). Phytases E and F increased Ca excretion, while phytase A reduced it (p < 0.01). Regardless of the phytase type, increasing the dietary Ca:Pavail ratio reduced (p < 0.05) the plasma P concentration and the excretion of total P and N and, conversely, increased (p < 0.05) the plasma concentration, intake and excretion of Ca. For the rearing period evaluated, it is possible to reduce the Pavail of the diet to 1.0 g/kg when Ca is maintained at 7.5 g/kg, and the diet is supplemented with 1500 FTU of phytase A, C, D or E/kg. This diet allows the maintenance of performance and adequate bone mineralization, and it improves the Ca, total P and Pphyt utilization in addition to reducing the excretion of N and P into the environment.  相似文献   

9.
Phytate, the mixed salt of phytic acid (myo-inositol hexaphosphate), derived from plant-sourced feed ingredients is invariably present in practical diets for pigs. Typically, swine diets contain in the order of 3.0 g kg− 1 phytate-bound phosphorus (phytate-P) but phytate concentrations are subject to variation. Importantly, phytate-P is only partially utilised by pigs because they do not generate sufficient endogenous phytase activity. Phytate-degrading enzymes, via step-wise dephosphorylation of phytate, have the capacity to liberate phytate-P, thus enhancing P absorption and reducing P excretion, which are both nutritionally and ecologically beneficial consequences. The commercial introduction of microbial phytases in 1991 has greatly magnified the interest in the roles of phytate and phytase in pig nutrition.

The capacity of microbial phytases to enhance growth performance of pigs offered diets with inadequate P levels is well documented. However, in some instances, phytase has been shown to improve performance of pigs offered P adequate diets thus phytase-induced improvements in growth performance should not be attributed entirely to increased P availability. This raises the possibility that phytase is increasing the utilisation of nutrients other than P. These so-called ‘extra-phosphoric’ effects of phytase remain controversial, particularly in relation to protein and amino acid availability. There are conflicting opinions that are reflected in the inconsistent outcomes of studies to determine the effect of phytase on ileal digestibility of amino acids and protein utilisation in pigs. In phytase amino acid digestibility assays, it seems likely the choice of chromic oxide as the dietary marker has contributed to these ambiguous results, which may be further complicated when ileal digesta samples are taken from cannulated pigs fed on a restricted, twice-daily basis. In order to resolve this critical issue, there is an urgent need to assess the impacts of selection of dietary markers, methods of ileal digesta collection and feeding regimen relative on the outcomes of phytase amino acid digestibility assays in pigs.

However, inconsistent results from phytase studies in pigs are not confined to amino acid digestibility assays. Arguably, insufficient attention has been paid to dietary substrate levels in relation to phytase inclusion from both scientific and practical standpoints. Phytate analyses are not straightforward and there is a real need to develop more accurate and rapid methods to facilitate phytate determinations. The properties of phytate vary between (and within) feed ingredients where solubility of phytate may be critical; which, in turn, is a function of gut pH in pigs. Contemporary phytases have the capacity to degrade approximately 50% of dietary phytate at the level of the ileum, which may mean higher inclusion rates are warranted. Consequently, there is scope for the development of more effective ‘second-generation’ phytate-degrading feed enzymes and their possible introduction, coupled with a better scientific understanding of relevant fundamental issues, will ensure that phytate-degrading enzymes will contribute to viable and sustainable pig production to an even greater extent in the future.  相似文献   


10.
The efficacy of an Escherichia coli-derived phytase preparation   总被引：1，自引：0，他引：1  
Five experiments were conducted to evaluate the effect of an Escherichia coli-derived phytase on phytate-P use and growth performance by young pigs. The first experiment involved time course, pH dependence, and phytase activity studies to investigate the in vitro release of P from corn, soybean meal, and an inorganic P-unsupplemented corn-soybean meal negative control diet. In Exp. 2, which was designed to determine the efficacy of the E. coli-derived vs. fungal phytase-added diets at 0, 250, 500, 750, 1,000, or 1,250 FTU/kg (as-fed basis; one phytase unit or FTU is defined as the quantity of enzyme required to liberate 1 micromol of inorganic P/min, at pH 5.5, from an excess of 15 microM sodium phytate at 37 approximately C) and a positive control diet, eight individually penned 10-kg pigs per diet (12 diets, 96 pigs) were used in a 28-d growth study. The third experiment was a 10-d nutrient balance study involving six 13-kg pigs per diet (four diets, 24 pigs) in individual metabolism crates. In Exp. 4, eight pens (four pigs per pen) of 19-kg pigs per treatment were used in a 42-d growth performance study to examine the effect of adding the E. coli-derived phytase to corn-soybean diets at 0, 500, or 1,000 FTU/kg (as-fed basis) and a positive control (four diets, 128 pigs). In Exp. 5, six 19-kg pigs per treatment were used in a 10-d nutrient balance study to investigate the effects of the E. coli-derived phytase added to diets at 0, 250, 500, 750, or 1,000 FTU/kg (as-fed basis) and a positive control diet (six diets, 36 pigs). The in vitro study showed that the E. coli-derived phytase has an optimal activity and pH range of 2 to 4.5. Inorganic phosphate release was greatest for soybean meal, least for corn, and intermediate for the negative control diet. Dietary supplementation with graded amounts of E. coli-derived phytase resulted in linear increases (P < 0.05) in weight gain, feed efficiency, and plasma Ca and P concentrations in 10-kg pigs in Exp. 2. Phytase also increased P digestibility and retention in the 13-kg pigs in Exp. 3. In Exp. 4, dietary supplementation with E. coli-derived phytase resulted in linear increases (P < 0.05) in weight gain and feed efficiency of 19-kg pigs. Supplementation of the diets of 19-kg pigs with the E. coli-derived phytase also improved Ca and P digestibility and retention in Exp. 5. In the current study, the new E. coli-derived phytase was efficacious in hydrolyzing phytate-P, both in vitro and in vivo, in young pigs.  相似文献   

11.
Heat stability of commercial preparations of phytase has been of concern for quite some time, and efforts have been made to develop new preparations of this enzyme. A study was conducted to determine the stability of commercially available phytase product (granulate; Phytase A) and the new experimental product (powder; Phytase B). In the in vitro study, incubation of 100 mg of each of Phytase A and B with 200 μl of buffer for 2 min (30 s at desired temperature) resulted in 27.6 and 10.4% loss of activity at 60 °C and 80.6 and 53.9% at 70 °C, respectively. Both enzyme products were further subjected to steam pelleting in feed mills located in Manitoba and British Columbia. In the Manitoba study, the temperature of pellets as recorded at the discharge averaged 67 °C and was similar to that determined in British Columbia (70 °C). Under such temperatures, which may have been lower than the actual temperatures within the pellet mill, the loss of endogenous phytase activity averaged 58.5% (from 451 to 187 U kg− 1) and 42.5% (from 287 to 165 U kg− 1) in the two mills, respectively. Following correction for endogenous phytase activity, Phytase A and B recovery averaged 36.4 and 49.9%, respectively, at the Manitoba site and 44.1 and 49.4% at the British Columbia site. It appears evident from this study that the heat stability of enzyme (protein) per se rather than the granulation technology is a primary factor determining the stability of microbial phytase during steam pelleting.  相似文献   

12.
Availability of phytate-bound P as influenced by supplemental phytase was studied in eight horses consuming four diets in a 4 x 4 Latin square design experiment. The treatments were a control (containing a low P level, 18.4 g/d) and three high-P diets. These diets contained P as monocalcium phosphate (MCP; 43.7 g/d), myoinositol hexakisphosphate in the form of wheat and rice bran (MIHP; 41.8 g/d), or MIHP with microbial phytase (MIHPP; 42.5 g/d). The proportions of phytate-bound P were 3, 1, 55, and 56% for the control, MCP, MIHP, and MIHPP, respectively. The MIHPP diet was supplemented with 300 phytase units (FTU)/kg (as-fed basis). Feces and urine were collected quantitatively and analyzed for P, Ca, and Mg. Urinary P excretion was lower (P < 0.05) with the control diet (0 g of P/d) than with the MCP diet (1.0 g of P/d). The low urinary P excretion (0.3 g of P/d) for the MIHP diet suggested low P availability compared with the MCP diet, but apparent digestibility of P expressed as a percentage of intake did not differ (P = 0.065) between these diets. Apparent Ca digestibility was lower (P < 0.05) for the MIHP diet than for the MCP diet (26.4 vs. 42.4%). This difference may have been caused by the origin of the Ca in these diets. Phytase supplementation increased apparent Ca digestibility from 26.4 to 31.5% (P < 0.05). Magnesium was not influenced by the level of phytate in the diet. Our data indicate that phytase supplementation had more influence on Ca digestibility than on P digestibility and suggest that phytase supplementation may be beneficial for improving Ca digestibility for horses receiving a phytate-rich diet.  相似文献   

13.
To determine the influence of incubation time, diet, and particle size on Ca and P solubility in vitro, experimental diets were formulated to contain 0.89% Ca and 0.40% available P (positive control; PC) or 0.76% Ca and 0.27% available P (negative control; NC). The PC was supplemented with 0 or 1,000 phytase units (FTU) of microbial phytase/kg and the NC with 0, 1,000, or 5,000 FTU/kg diet of microbial phytase for a total of 5 experimental diets. In Exp. 1, diets were exposed to simulated gastric digestion containing HCl and pepsin for 42 min, or a small intestinal digestion phase containing NaHCO(3) and pancreatin for 60 min. In Exp. 2, diets were ground to pass a 1- or 2-mm screen and exposed to gastric digestion for 5, 10, or 20 min. Phosphorus and Ca solubility were similarly influenced by diet and digestion phase (Exp. 1), and there was no interaction. Phytase supplementation improved (P < 0.001) Ca and P solubility in both the PC and NC diets (Exp. 1) and increased P (P < 0.001) and Ca (P < 0.001) solubility in the gastric phase of the in vitro digestion model (Exp. 2). Phytase continued to release P in the gastric test over time, which resulted in a diet × time interaction (P < 0.05). Calcium solubility reached an asymptote at 5 min and both Ca and P solubility was reduced (P < 0.05) in diets ground to pass a 2 mm screen compared with diets ground to pass a 1-mm screen. In addition, P and Ca solubility did not change over time in diets not supplemented with phytase. In conclusion, phytase or particle size altered the kinetics of Ca and P release in a non-parallel fashion, which may be associated with the precipitation of Ca with phytate and the sequential dephosphorylation of phytate by a microbial 6-phytase. In the presence of phytase, considerable Ca and P hydrolysis occurred within 5 min of a simulated gastric digestion. However, the solubility of Ca and P reached a plateau in the gastric phase of digestion and no further improvements in solubility are apparent in the small intestine. Therefore, absorption of Ca and P may be complicated by conditions within the gastrointestinal tract, particle size, precipitation with anti-nutrients, and differential rates of delivery to the small intestine.  相似文献   

14.
A performance trial was conducted with broiler chicks to study the effect of phytase (PHY) supplementation in diets formulated with reduced AME, Ca, and P. The nutrient digestibility was determined during the 14- to 21-d and 28- to 35-d periods. The treatments consisted of 3 diets (NC1, NC2, NC3) differing in nutrient content and each diet with or without supplemental PHY (NC1, 0 or 500; NC2, 0 or 750; NC3, 0 or 1,000 U of PHY/kg feed) and 1 positive control diet (PC). Compared with the PC diet, negative control diets (NC) resulted in lower AME and apparent ileal amino acid digestibility for some amino acids. Phytase supplementation of the NC diets increased AME, apparent ileal amino acid digestibility, and apparent ileal crude protein digestibility. Phytase addition also increased mineral absorption in 21- and 35-d-old broilers fed NC diets. Reduced nutrient digestibility appears to be a factor in the weight gain and feed intake results. Reducing Ca and P content reduced feed intake in a stepwise fashion in the NC diets. Phytase increased feed intake and generally improved nutrient digestibility, which resulted in an increase in digestible nutrient intake. Averaged across NC diets, PHY improved body weight. Bone-breaking strength was the most consistent predictor of Ca and P reduction. All NC diets had significantly lower bone-breaking strength than the PC. Phytase supplementation of the NC diets gave bone-breaking strengths that were comparable to the PC. Diets with PHY had the highest bioeconomic index.  相似文献   

15.
The effectiveness of an Escherichia coli phytase in comparison with a commercially available Aspergillus phytase in improving the bioavailability of phosphorus in broilers, layers and young pigs was studied in three separate experiments. Three basal diets, marginally deficient in dietary P mainly provided as phytate, were formulated. Both phytases were added to the diets at the rate of 500 U/kg diet. The phytases significantly (P < or = 0.05) improved the availability of phytate P to broilers, layers and young pigs. Aspergillus and E. coli phytases enhanced the pre-caecal digestibility of P by 11 and 29% for broilers and 18 and 25% for layers, respectively. Total tract digestibility of P (P balance) was also enhanced but with smaller magnitude. In pigs, total tract digestibility of P was improved by 33 and 34% by Aspergillus and E. coli phytases, respectively. Under the conditions of this study, it was observed that E. coli consistently, though with small magnitude in layers and pigs, enhanced the availability of phytate P at the same range or slightly better than Aspergillus phytase. It was only in pigs that the availability of Ca was significantly (P < or = 0.05) improved by addition of both phytases. It can be concluded that E. coli phytase is highly effective in improving the bioavailability of phytate P to broilers, layers and young pigs. This seems to be based on the high proteolytic stability of the enzyme in the digestive tract, as shown recently.  相似文献   

16.
H.D. Poulsen   《Livestock Science》2007,109(1-3):247-250
Due to environmental concern a lot of effort has been dedicated to improve the utilisation of phytate phosphorus (P) in cereals and seeds. Consequently, microbial phytases are added to pig diets at the expense of feed phosphates. However, there is still a need for inclusion of feed phosphate, especially in diets for young piglets and lactating sows in order to meet their P requirement. This creates a demand for feed phosphates with a high P availability. Fourteen feed phosphates, including two newly developed monocalcium phosphates (MCP), were tested in this experiment. For each source, a series of 5 diets was prepared by inclusion of increasing amounts of either MCP or MSP. These diets were offered to pigs kept in metabolism crates (6 pigs on each of the 5 diets). After 5 days adaptation, total collection of faeces and urine was performed for 7 days. P availability in each source was determined by regression of the net absorption of P on P-intake. The experiment revealed that MSP had the highest availability (79%) and the availability of all the tested phosphates varied between 50 and 79%. The new MCPs proved to be 71 and 75% available.  相似文献   

17.
The objective of this study was to measure apparent total tract digestibility (ATTD) of Ca and P as well as reproductive performance in late gestation and lactating sows supplemented with a novel phytase and to compare the response to phytase supplementation between late gestation and lactating sows. A total of 45 late gestation sows and 45 lactating sows were used in experiments 1 and 2, respectively, in a completely randomized design. The sows were provided with a control diet or the control diet supplemented with 187.5 or 375 FYT phytase/kg feed for 10 days. The diets were prepared according to the formulas in use for production but without any inorganic P supplement. Titanium dioxide was included at 3 g/kg feed as an indigestible marker. Each dietary treatment was replicated with 15 sows individually housed in farrowing stalls. The sows were allowed to adapt to the experimental diets for 5 days before a 5-d fecal collection by grab sampling, and the performance of the sows and their litters were measured until weaning. The results showed that the ATTD of Ca increased linearly (P < 0.001), while the ATTD of P increased both linearly and quadratically (P < 0.01) with increasing supplementation of phytase in both late gestation and lactating sows. There was no significant effect of phytase on the ATTD of dry matter, crude protein, and gross energy, and the performance of the sows and their progenies. The phytase added at 187.5 and 375 FYT/kg feed released 0.07% and 0.10% digested P, respectively, in late gestation sows, which compared with 0.09% and 0.12% digested P in lactating sows. In conclusion, a novel phytase at 187.5–375 FYT/kg feed could release 0.07–0.12% digestible P for sows. It appeared that using the P digestibility values of feed ingredients listed by NRC to formulate a diet for sows might overestimate dietary P supply and a greater response to phytase supplementation could be expected in lactating sows than in late gestation sows.  相似文献   

18.
植酸酶的研究进展   总被引：21，自引：0，他引：21  
植酸酶是能降解饲料中植酸及其盐的酶。它能提高磷利用率,解除植酸对一些矿物元素如钙、锌、铁、铜等的抗营养效应,不仅对动物具有良好的增重效果,同时可降低动物排泄磷量,有利于环境保护。植酸酶作为饲料添加剂其作用效果受到饲料中钙、磷水平和钙磷比例以及维生素Ｄ含量的影响。植酸酶的运用需要降低饲料中钙、磷水平,维生素Ｄ与植酸酶之间可能存在协同效应。对植酸酶运用的经济分析表明,使用植酸酶能代替饲料中需添加的无机磷。  相似文献   

19.
The present study gives an overview on the whole mechanism of phytate degradation in the gut and the enzymes involved. Based on the similarity of the human and pigs gut, the study was carried out in pigs as model for humans. To differentiate between intrinsic feed phytases and endogenous phytases hydrolysing phytate in the gut, two diets, one high (control diet) and the other one very low in intrinsic feed phytases (phytase inactivated diet) were applied. In the chyme of stomach, small intestine and colon inositol phosphate isomers and activities of phytases and alkaline phosphatases were determined. In parallel total tract phytate degradation and apparent phosphorus digestibility were assessed. In the stomach chyme of pigs fed the control diet, comparable high phytase activity and strong phytate degradation were observed. The predominant phytate hydrolysis products were inositol phosphates, typically formed by plant phytases. For the phytase inactivated diet, comparable very low phytase activity and almost no phytate degradation in the stomach were determined. In the small intestine and colon, high activity of alkaline phosphatases and low activity of phytases were observed, irrespective of the diet fed. In the colon, stronger phytate degradation for the phytase inactivated diet than for the control diet was detected. Phytate degradation throughout the whole gut was nearly complete and very similar for both diets while the apparent availability of total phosphorus was significantly higher for the pigs fed the control diet than the phytase inactivated diet. The pathway of inositol phosphate hydrolysis in the gut has been elucidated.  相似文献   

20.
Four chick trials and one pig trial were conducted to investigate the phosphorus-releasing efficacy oftwo commercial phytase enzymes (Natuphos and Ronozyme) and an experimental E. coli phytase enzyme (ECP) when added to corn-soybean meal diets containing no supplemental inorganic P (iP). In the 13- or 14-d chick trials, three or four graded levels of iP (0, 0.05,0.10,0.15%) from KH2PO4 were added to the basal diet to construct standard curves from which bioavailable P release could be calculated for the phytase treatments. In all cases, phytase supplementation levels were based on an assessment of phytase premix activity (i.e., P release from Na phytate at pH 5.5). Linear (P < 0.01) responses in tibia ash and weight gain resulted from iP supplementation in all assays. In the first chick trial, supplementation of 500 phytase units (FTU)/kg of ECP resulted in superior (P < 0.01) weight gain and tibia ash values compared with 500 FTU/kg of Natuphos. Results of the second chick trial revealed P-release values of 0.032 and 0.028% for 500 FTU/kg Natuphos and Ronozyme, respectively, and these were lower (P < 0.01) than the 0.125% P-release value for 500 FTU/kg of ECP. Tibia ash responded quadratically (P < 0.05) in response to graded levels of ECP up to 1,500 FTU/kg in the third chick trial. Combining Natuphos with either Ronozyme or ECP in Chick Trial 4 revealed no synergism between phytases with different initiation sites of P removal. The pig trial involved 10 individually fed weanling pigs per diet, and and phytase enzymes were supplemented to provide 400 FTU/kg in diets containing 0.60% Ca. Based on the linear regression of fibula ash on supplemental iP intake (r2 = 0.87), P-release values were 0.081% for Natuphos, 0.043% for Ronozyme, and 0.108% for ECP. These trials revealed an advantage of the E. coli phytase over the commercial phytases in young chicks.  相似文献   

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