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1.
低温弱光对茄子嫁接苗和自根苗抗氧化酶系统的影响 总被引：2，自引：0，他引：2
研究茄子自根苗和以番茄为砧木的嫁接苗耐低温弱光性和叶片活性氧清除系统的差异.结果表明,低温弱光胁迫下(8℃,80 μmol/(m2·s)),嫁接苗耐冷性明显高于自根苗.在低温弱光胁迫下,嫁接苗冷害指数、电解质渗透率、丙二醛(MDA) 含量明显低于自根苗;嫁接苗和自根苗叶片SOD,GPX,APX活性均上升,嫁接苗上升幅度低于自根苗;嫁接苗和自根苗CAT活性下降,嫁接苗下降幅度低于自根苗.嫁接改善茄子对低温弱光耐性的机理可能在于其使抗氧化酶敏感度降低,减轻了低温弱光的膜酯过氧化程度,从而降低了电解质渗透率,提高了耐冷性. 相似文献
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试验表明，低温弱光胁迫下嫁接苗耐冷性明显高于自根苗；在低温胁迫下嫁接苗冷害指数、电解质渗透率、丙二醛（MDA）含量明显低于自根苗；嫁接苗和自根苗SOD、GPX、APX活性均上升，但嫁接苗上升幅度低于自根苗；嫁接苗和自根苗CAT活性下降，但嫁接苗下降幅度低于自根苗。嫁接改善茄子对低温弱光耐性的机理可能在于其改变了抗氧化酶系统，减轻了低温弱光的膜酯过氧化程度，从而降低了电解质渗透率，提高了耐冷性。 相似文献
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不同耐盐性的黄瓜接穗嫁接后在NaCl胁迫下的生理响应 总被引：1，自引：0，他引：1
采用早多佳和津春2号黄瓜作接穗,耐盐的超级拳乇作砧木嫁接,以自根苗为对照,对不同接穗嫁接黄瓜苗在NaCI胁迫下的生长及生理特性进行了测定.结果表明:在100 mmol/L的NaCI胁迫下,早多佳嫁接苗叶面积和茎粗减少的程度比津春2号嫁接苗轻,脯氨酸含量、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、抗坏血酸过氧化物酶(APX)活性显著高于津春2号嫁接苗;但NaCI胁迫下早多佳嫁接苗的根干质量、地上部干质量、可溶性糖含量、可溶性蛋白质含量、含水量、SOD活性、MDA含量与津春2号嫁接苗无显著差异.相同NaCI胁迫条件下,早多佳自根苗的根干质量显著大于津春2号自根苗,地上部含水量、SOD活性显著高于津春2号自根苗,MDA含量显著低于津春2号自根苗.嫁接减小了不同黄瓜品种之间耐盐性的差异. 相似文献
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外源AsA对大蒜试管苗玻璃化及过氧化特征的影响 总被引：1，自引：0，他引：1
以大蒜品种二水早为材料,研究不同浓度外源AsA(抗坏血酸)对大蒜试管苗玻璃化发生及生理生化变化的影响。结果表明,在不同浓度外源AsA作用下,大蒜玻璃化试管苗百分率、MDA(丙二醛)含量、电解质渗透率明显低于对照,叶绿素含量及SOD、POD和CAT活性与对照相比则有升高的趋势。在同一浓度AsA下,大蒜玻璃化试管苗的组织含水量、MDA含量、电解质渗透率,SOD、POD和CAT活性均显著高于大蒜正常试管苗,叶绿素含量低于正常试管苗。说明外源AsA可以有效抑制大蒜试管苗玻璃化现象发生。 相似文献
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研究了100mmol/L NaCl胁迫对嫁接茄子幼苗生长、抗氧化酶活性和渗透调节物质含量的影响。结果表明:NaCl胁迫下,嫁接苗生长受抑制程度显著低于自根苗;嫁接苗超氧物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性以及脯氨酸和可溶性糖含量均显著上升,以上指标均显著高于自根苗;MDA含量和O2.-产生速率显著增加,嫁接苗含量显著低于自根苗。以上结果表明:NaCl胁迫下嫁接苗有较高的抗氧化酶活性和较低的氧化损伤,从而增强茄子的耐盐性。 相似文献
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不同砧木嫁接对NaCl胁迫下黄瓜幼苗生长、渗透调节和酶活性的影响 总被引：1，自引：0，他引：1
采用'超丰8848'和'黑籽南瓜'作砧木,'津春2号'黄瓜作接穗嫁接,以'津春2号'自根苗为对照,研究了NaCl胁迫时不同砧木嫁接黄瓜苗生长及生理特性的影响.结果表明,NaCl胁迫下,嫁接苗地上部和根干重、叶面积、茎粗受抑制的程度显著低于自根苗;嫁接苗叶片中MDA、H2O2含量、超氧阴离子产生速率显著低于自根苗,而叶片中可溶性糖含量、地上部含水量、SOD、POD、CAT、APX活性显著高于自根苗.NaCl胁迫下,'超丰8848'嫁接苗生长受抑制程度、叶片中MDA、H2O2含量显著低于'黑籽南瓜'嫁接苗,脯氨酸含量、可溶性蛋白质含量、地上部含水量、SOD、POD、CAT、APX、GR活性显著高于'黑籽南瓜'嫁接苗.具有更好的渗透调节和抗氧化能力是'超丰8848'嫁接苗耐盐性高于'黑籽南瓜'嫁接苗的重要原因. 相似文献
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不同砧木对嫁接黄瓜细胞保护酶活性和丙二醛含量的影响 总被引：3，自引：0，他引：3
将欧洲型黄瓜品种春秋王和华南型黄瓜品种沪杂6号分别嫁接到4种不同的砧木上,对嫁接黄瓜和自根黄瓜叶片2种细胞保护酶(SOD和POD)活性的变化及丙二醛(MDA)含量进行分析。结果表明,不同砧木嫁接处理后SOD和POD的活性都呈先升高后下降的趋势,第3周时达到最大,且春秋王和沪杂6号嫁接苗叶片中2种酶的活性水平都高于自根苗,而自根苗中MDA含量高于所有嫁接苗;不同砧木之间保护酶活性和MDA差异不显著。 相似文献
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NaCl胁迫对嫁接西瓜抗氧化酶及叶绿素荧光参数的影响 总被引：1，自引：1，他引：0
为研究盐胁迫下嫁接西瓜的耐盐机制,以耐盐性较强的葫芦品种超丰抗生王为砧木,耐盐性较弱的西瓜品种秀丽为接穗,采用营养液水培法,研究了NaCl胁迫对西瓜自根苗和嫁接苗谷胱甘肽抗氧化酶系统、SOD同工酶、POD同工酶及叶绿素荧光参数的影响。结果表明:NaCl胁迫下嫁接苗、自根苗叶片中超氧阴离子和H2O2含量明显增加;嫁接苗叶片和根系中SOD、POD同工酶变化明显不同,不同同工酶活性差异明显。此外,NaCl胁迫下嫁接苗叶片非酶系统抗氧化酶[抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)和脱氢抗坏血酸还原酶(DHAR)]活性均显著高于自根苗;嫁接苗叶片中叶绿素荧光参数qP和Fv/Fm降低的幅度小于自根苗。上述结果表明,NaCl胁迫下嫁接苗通过维持较高的抗氧化酶活性以提高活性氧的清除能力,并保持较高的光合效率,从而增强西瓜幼苗对盐胁迫的耐性。 相似文献
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Chilling Tolerance and Physiological Parameters as Influenced by Grafting in Watermelon Seedlings 总被引：1，自引：1，他引：0
The influences of different rootstocks on chilling tolerance and physiological parameters in wa-termelon seedlings have been studied. The results showed that grafting improved the chilling tolerance. Com-pared with own-rooted watermelon seedlings, the grafted watermelon seedlings had lower chilling injury index,lower electrolytic leakage (%), lower malondialdehyde (MDA) content, higher chlorophyll and proline con-tent, and higher activities of superoxide dismutase (SOD), ascorbate peroxidase (AsA-POD) and de-hydroascorbate reductase (DR) in the leaves under iow temperature stress. There was a considerable differenceof chilling tolerance among different grafted watermelon seedlings due to the difference of rootstock chillingtolerance. After low temperature treatment, the grafted seedling with higher chilling tolerance had lower elec-trolytic leakage ( %), lower MDA content, higher proline content and higher activities of SOD, AsA-POD andDR in the leaves compared with the grafted seedling with weaker chilling tolerance. From these, we could con-clude that chilling tolerance of watermelon seedlings may be related to higher antioxidative ability and mem-brane stability in the plants. The chilling tolerance of grafted seedling could be properly evaluated by compre-hensive physiological indexes but not a single physiological index. 相似文献
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The aim of the study is to determine whether grafting could improve antioxidant enzyme activities and polyamine contents in leaves of cucumber plants（Cucumis sativus L.cv.Xintaimici） under copper stress.Grafted（using Cucurbita ficifolia as rootstock） and ungrafted cucumber seedlings were cultured in deep flow technique（DFT） with the Cu2＋ concentration of 40 μmol L-1.The results showed that on the 9th day of copper stress treatment,the contents of malondialdehyde（MDA） and hydrogen peroxide（H2O2）,superoxide radical（） producing rate,and electrolyte leakage percentage were significantly lower in grafted seedlings in comparison to those of the ungrafted seedlings,whereas the activities of antioxidants such as superoxide dismutase（SOD,EC 1.15.1.1）,peroxidase（POD,EC 1.11.1.7）,ascorbate peroxidase（APX,EC 1.11.1.11）,catalase（CAT,EC 1.11.1.6）,glutathione reductase（GR,EC 1.6.4.2）,and monodehydroascorbate reductase（MDHAR,EC 1.6.5.4） of grafted seedlings were also significantly higher than that of ungrafted seedlings,and the contents of free proline and soluble protein of grafted seedlings were significantly higher than that of ungrafted seedlings.Cu2＋ treatment increased the putrescine（Put） level and lowered the spermidine（Spd） and spermine（Spm） levels,thereby reducing the Put/（Spd ＋ Spm） ratio in leaves of grafted and ungrafted seedlings.Grafting markedly reversed these Cu-induced effects for all three PAs and partially restored the Put/（Spd ＋ Spm） ratio in leaves.These results suggest that grafting can enhance the tolerance of cucumber seedlings to Cu2＋ stress by increasing the activities of antioxidants and the levels of endogenous Spd and Spm,decreasing the Put/（Spd ＋ Spm） ratio and the levels of ROS,promoting free proline and soluble protein synthesis in cucumber seedling leaves. 相似文献
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The roots of figleaf gourd （Cucurbita ficifolia, as rootstock） could improve the resistance of cucumber plants （Cucumis sativus L. cv. Jinyan 4, as scion） to low temperature. In this experiment, the root activity and photosynthetic activity of photosystems in the own-rooted and grafted-cucumber plants were studied at chilling temperature （4℃） under low irradiance （100 μmol m^2 s^-1 PFD）. Compared with dark adaptation seedlings, the chlorophyll a fluorescence transient curve and the oxidizable P700 （P700＋） of both the own-rooted and grafted seedlings decreased, and PS2 and PS1 of the own-rooted seedling leaves were more inhibited than that of grafted ones at the end of chilling stress. The reduced triphenyltetrazolium chloride （TTC）, which was used to reflect the root activity, kept stable in grafted seedling roots at the end of chilling stress, while it decreased noticeably in the own-rooted seedling roots. These results implied that the root system activity of the grafted seedling roots was higher than that of the own-rooted ones. Superoxide dismutase （SOD） activity was higher in both the grafted seedling roots and leaves than that in own-rooted seedlings at both room temperature and chilling temperature. Upon exposure to chilling stress, the malondialdehyde （MDA） content, which reflects the degree of lipid peroxidation, increased markedly in the own-rooted seedling roots and leaves and kept stable in the grafted-cucumber seedlings. 相似文献
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Effects of Exogenous Silicon on the Activities of Antioxidant Enzymes and Lipid Peroxidation in Chilling-Stressed Cucumber Leaves 总被引：2，自引：0，他引：2
In order to increase vegetable productivity by improving environmental conditions, this article investigates the effects of exogenous silicon on the activities of major antioxidant enzymes and on lipid peroxidation under chilling stress, and it examines whether silicon-induced chilling tolerance is mediated by an increase in antioxidant activity. Cucumis sativus cv. Jinchun 4 was hydroponically cultivated to the two-leaf stage, at which point seedlings were watered with different concentrations of silicon （0, 0.1 and 1 mmol L^-1） and separately exposed to normal （25/18℃） or chilling （15/8℃） temperatures for six days under low light （100μmol m^-2 s^-9. Data were collected from the second leaves on the percentage of withering and the levels of endogenous silicon, malondialdehyde （MDA）, hydrogen peroxide （H202）, superoxide radical （O2^.-）, superoxide dismutase （SOD, EC 1.15.1.1）, glutathione peroxidase （GSH-Px, EC 1.11.1.9）, ascorbate peroxidase （APX, EC 1.11.1.11）, monodehydroascorbate reductase （MDHAR, EC 1.6.5.4）, glutathione reductase （GR, EC 1.6.4.2）, reduced glutathione （GSH） and ascorbate （AsA）. Compared to normal temperatures, chilling resulted in partially withered leaves and increased MDA content. When 0.1 or 1 mmol L^-1 exogenous silicon was combined with chilling, the withering of the cucumber leaves was reduced relative to the original chilling treatment, while the endogenous silicon content was increased, antioxidants such as SOD, GSH-Px, APX, MDHAR, GR, GSH, and AsA were more active, and the levels of H2O2, O2^.-, and MDA were lower. We propose that exogenous silicon leads to greater deposition of endogenous silicon and thereby increases antioxidant activities and reduces lipid peroxidation induced by chilling. 相似文献
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An experiment was carried out to determine plant growth, mineral uptake, lipid peroxidation, antioxidative enzymes, and antioxidant of cucumber plants （Cucumis sativus L. cv. Xintaimici） under copper stress, either ungrafted or grafted onto the rootstock （Cucurbitaficifolia）. Excess Cu inhibited growth, photosynthesis, and pigment synthesis of grafted and ungrafted cucumber seedlings and significantly increased accumulation of Cu in roots besides reducing mineral uptake. Cu concentration in roots of grafted cucumber plants was significantly higher than that of ungrafted plants and obviously lower in leaves. The accumulation of reactive oxygen species （ROS） significantly increased in cucumber leaves under Cu stress and resulted in lipid peroxidation, and the levels of ROS and lipid peroxidation were greatly decreased by grafting. Activities of protective enzymes （superoxide dismutase, SOD; peroxidase, POD; catalase, CAT; ascorbate peroxidase, APX; dehydroascorbate reductase, DHAR; glutathione reductase, GR） and the contents of ascorbate and glutathione in leaves of grafted plants were significantly higher than those of ungrafted plants under Cu stress. Better performance of grafted cucumber plants were attributed to the higher ability of Cu accumulation in their roots, better nutrient status, and the effective scavenging system of ROS. 相似文献
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[目的]分析低温胁迫下苦瓜苗期生理变化与耐冷性评价的关系,筛选适宜苦瓜苗期耐冷性评价的取材时期,为提高苦瓜耐冷性评价的准确性和可靠性提供参考依据.[方法]对已知耐冷性存在差异的苦瓜材料MC108、MC45和MC6幼苗进行10℃低温胁迫,分别测定其叶片在胁迫0、2、4、6、8和10 d时的生理指标,绘图显示生理指标的变化情况,结合隶属函数值对各胁迫时期苦瓜耐冷性的评价结果,筛选适宜苦瓜苗期耐冷性评价的取材时期.[结果]耐冷性存在差异的苦瓜材料对冷害胁迫反应机制不同,与低温胁迫0 d(对照)相比,低温胁迫第10 d时冷敏材料MC6的可溶性蛋白含量降幅较大,脯氨酸(Pro)含量增加量更多;与MC45和MC6相比,低温胁迫0、2、4、6、8和10 d时耐冷材料MC108的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性变幅相对较小.除丙二醛(MDA)含量峰值出现的时间不同外,MC108、MC45和MC6的Pro、 可溶性蛋白含量及SOD、POD活性降低的共同时间点均为低温胁迫处理第10 d.结合隶属函数值对苦瓜耐冷性的评价结果判断,低温胁迫处理第10 d的叶片生理指标适宜作为其苗期耐冷性评价指标.[结论]苦瓜材料幼苗进行10℃低温胁迫处理第10 d是进行苗期耐冷性评价的适宜取材时期. 相似文献
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低温下外源水杨酸对水稻幼苗抗氧化酶系的影响 总被引：15，自引：0，他引：15
用水杨酸(SA)预处理水稻幼苗,能够提高水稻幼苗的抗寒性。0.5 mmol/L SA预处理水稻幼苗降低了低温胁迫期间水稻幼苗体内过氧化氢(H2O2)和丙二醛(MDA)的含量及超氧阴离子(O2-.)的产生速率;同时酶活性分析表明,SA预处理后水稻幼苗在低温胁迫下超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性较低温对照上升,而抗坏血酸过氧化物酶(APX)活性较低温对照下降。研究结果表明,常温下用0.5 mmol/L SA预处理水稻幼苗,提高了抗氧化酶的活性,从而提高了水稻幼苗的抗寒性。 相似文献
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24-表油菜素内酯对低温胁迫下葡萄幼苗抗氧化系统 及渗透调节物质的影响 总被引：3，自引：0，他引：3
【目的】探讨外源EBR（24-表油菜素内酯）对低温胁迫下葡萄幼苗抗氧化系统及渗透调节物质的影响。【方法】以欧亚种（Vitis vinifera L.）鲜食葡萄品种‘紫脆无核’营养袋扦插苗为试材,分别在4℃和0℃低温条件下,研究外源EBR对葡萄幼苗4种抗氧化酶活性（CAT、SOD、POD及APX）、超氧阴离子（ ）和丙二醛（MDA）含量以及渗透调节物质游离脯氨酸、可溶性糖和可溶性蛋白含量的影响。【结果】在两种低温胁迫条件下,外源EBR处理均使葡萄叶片抗氧化酶CAT、POD、SOD及APX活性升高,且总体与对照之间差异显著;同时也使葡萄叶片抗氧化物质GSH及AsA含量升高,在4℃和0℃条件下,GSH平均含量较对照分别升高5.4%及7.8%,AsA含量分别升高8.8%及13.0%;EBR处理使叶片 及MDA含量降低, 平均较对照分别降低28.5%及17.3%,MDA含量分别降低6.8%及7.5%;EBR处理明显提高了葡萄幼苗叶片脯氨酸、可溶性糖及可溶性蛋白含量,平均含量较对照分别提高8.4%和59.1%、3.5%和5.2%及18.6%和25.0%。【结论】在低温胁迫下,外源EBR通过提高抗氧化酶活性、抗氧化物质含量及渗透调节物质含量,减少了活性氧(ROS)的积累及膜脂过氧化产物MDA的产生,增强了葡萄幼苗耐低温胁迫的能力。 相似文献