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W Halangk G Gründel U Tr?ger K Zettl 《Archiv fuer experimentelle veterinaermedizin》1990,44(4):533-541
The simple method of retrograde flushing of spermatozoa from the epididymal cauda of slaughter bulls yielded an average of 2 x 10(9) spermatozoa from one cauda. The plasma membrane was intact in something between 80 and 85 percent of all epididymal spermatozoa. Respiratory rates and motility were clearly below values typical of ejaculated spermatozoa, though sizeable differences were found to exist for both parameters between individual preparations. The endogenous substrates available proved to be sufficient for such low metabolic activity, whereas higher energy turnover required the presence of exogenous substrates, such as lactate, pyruvate or fructose. Respiratory capacity, determined by uncoupling of oxidative phosphorylation in the presence of lactate, was comparable to that of ejaculated bull spermatozoa. The above findings are likely to suggest that the lower respiratory rates of epididymal spermatozoa were attributable to the fact that their consumption for motility of adenosine triphosphoric acid (ATP) was lower than that of ejaculated spermatozoa. 相似文献
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Toyonaga M Morita M Hori T Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(6):827-829
Glycoproteins (GPs) are known to be involved in the phenomenon of sperm maturation and capacitation. In the present study, we investigated the attachment of GPs on sperm cell membrane during the process of feline sperm maturation from testicular sperm to ejaculated sperm by using 8 FITC-labeled lectins. The results showed that 3 types of GPs were presented on testicular sperm and 7 on caput epididymal sperm. Corpus and cauda epididymal sperm and ejaculated sperm had GPs detected by 8 FITC-labeled lectins used in the present study. This study demonstrates the part of the characteristic of GPs that are present on the feline sperm cell membrane during the process of sperm maturation. 相似文献
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Brüssow KP Torner H Rátky J Manabe N Tuchscherer A 《The Journal of reproduction and development》2006,52(2):249-257
In the pig, a temporal relationship is suggested between sperm release from the sperm reservoir (SR) and ovulation, but the mechanism(s) is still under discussion. In two experiments, the influence of transferred ova on the release of SR-spermatozoa at ovulation and the effect of supplementation with non-sulfated glycosaminoglycan hyaluronan (HA) on embryo development and the number of accessory spermatozoa, respectively, were examined. PMSG/hCG primed ovectomized gilts that had previously received endoscopic low-dose insemination into the cranial uterine horn were used as an experimental model. After salpingectomy, tubal segments (ampulla, cranial, and caudal isthmus) were flushed and sperm numbers or respective accessory spermatozoa were counted. In Experiment 1, the distribution of the sperm population was altered in the presence of cumulus-oocyte-complexes (COCs). A higher proportion of spermatozoa was found after transfer of COCs into one oviduct in the ampulla and cranial isthmus segments compared with the controls (17.5 vs. 4.9%, p<0.05). In Experiment 2, the quality of the transferred ova and treatment influenced the presence of accessory spermatozoa. Transfer of COCs together with HA increased (p<0.05) the number of accessory spermatozoa compared with the other treatment groups and was similar to those in the "undisturbed" controls. No modifications were obtained regarding mean blastomere numbers (2.6 +/- 0.2 to 3.1 +/- 0.2). In summary, this study was demonstrated that cumulus-oocyte-complexes may be involved in triggering sperm release from the pig oviductal SR and that HA might be related to sperm release. 相似文献
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A method has been developed for the collection of ejaculate from anaesthesized dog foxes. General anaesthesia was produced by inhaling 3 to 5% narcotan mixed with oxygen, the inhalation rate being two litres per minute. An electroejaculator with a bipolar electrode, introduced in the rectum, was used for the electrostimulation of ejaculation. The voltage needed to produce the ejaculation effect and the number of impulses depended on the individuality of the foxes. The average amount of ejaculate per one collection was 0.86 ml, the sperm motility percentage was 74.80% sperm concentration was 270.62 x 10(3) spermatozoa per mm3, and the average pH value was 6.87. 相似文献
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Katarzyna Andraszek Dorota Banaszewska Olga Szeleszczuk Piotr Niedbała Marta Kuchta-Gładysz 《Reproduction in domestic animals》2020,55(4):515-522
Sperm cells isolated from the tail of the epididymis and from the semen of the same individuals were analysed. The use of silver nitrate to stain sperm cells isolated from the tail of the epididymis made it possible to identify structures that were not visible in the sperm from semen. Silver nitrate very clearly distinguished the acrosomal and distal parts of the sperm head. Following silver nitrate staining, the sperm isolated from the tail of the epididymis were characterized by dark ‘collars’ in the distal part of the head. These ‘collars’ are not visible in the sperm cells isolated from semen. The results of the study indicate differences in the dimensions of sperm isolated from the tail of the epididymis and sperm in semen. Sperm isolated from the tail of the epididymis had smaller heads, despite their longer length, and had longer midpieces and tails than ejaculate sperm. Silver nitrate staining is a simple and fast technique. Silver nitrate makes it possible to identify the acrosome and post-acrosomal region of the sperm head and to clearly identify the midpiece. Therefore, it can be successfully used to supplement routine techniques for evaluating sperm morphology or as an independent technique. 相似文献
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Identification of sperm morphometric subpopulations in cooled‐stored canine sperm and its relation with sperm DNA integrity 
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下载免费PDF全文 The aims of this study were to (i) identify different morphometric subpopulations in cooled‐stored canine sperm and their patterns of distribution during cool‐storage for up to 240 hr and (ii) determine whether or not morphometric sperm subpopulations (sP) are related to sperm DNA integrity. For that purpose, morphometric parameters were analysed by computer‐assisted sperm analysis (CASA) and sperm DNA fragmentation (sDFi) using the sperm Halomax test. Four morphometric sperm heads subpopulations were identified: sP1 (large and rounded), sP2 (large and elongated), sP3 (small and rounded) and sP4 (small and elongated). sP1 was the most predominant subpopulation for up to 72 hr and thereafter sP3 increased progressively. sDFi increased after 48 hr of cool‐storage. Although sP3 showed a positive correlation with sDFi, and both increased over time, it could not be ensured that only the sperm with fragmented DNA are accumulated in sP3. In conclusion, sP3 and DNA fragmentation increased progressively during cool‐storage, becoming possible indicators of sperm damage. However, it cannot be concluded that sP3 only contains sperm with fragmented DNA. 相似文献
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Takahiro AYA Yukiko TOMIOKA Takashi TAKEUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1144
The effect of lactoferrin (LF) on embryo development was investigated by using lipopolysaccharide (LPS)-treated mouse sperm. For the development rate of the 2-cell stage embryo, the embryo derived from LPS- and LF-treated sperm showed similar survival rate to the control embryo. On day 12 after the embryo transfer into the recipient, the frequent abnormality was observed in the embryo derived from LPS-treated sperm, and the abnormality was tended to be inhibited in the embryo derived from LPS- and LF-treated sperm. These results imply that LF treatment on sperm contaminated with bacteria may facilitate the embryo development, which contribute to the improvement of infertility. 相似文献
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实验探讨了精卵因素对母牛卵母细胞质内精子显微注射效果的影响.结果表明:新鲜精液组和性控冻精组ICSI对卵母细胞存活率、卵裂率和囊胚率均无显著差异(P>0.05);采用A、B、C级精子穿刺Ⅰ、Ⅱ、Ⅲ级卵母细胞时,穿刺卵受精率、卵裂率和囊胚率在三者之间无显著差异(P>0.05),总体效果A>B>C、Ⅰ>Ⅱ>Ⅲ;D级精子穿刺Ⅱ级卵母细胞后的穿刺卵受精率显著低于Ⅰ级卵母细胞(P<0.05),卵裂率和囊胚率与Ⅰ级卵母细胞相比无显著差异(P>0.05),穿刺Ⅲ级卵母细胞后的穿刺受精率和囊胚率均显著低于Ⅰ级卵母细胞(P<0.05),受精卵的卵裂率与Ⅰ级卵母细胞相比则无显著差异(P>0.05).由此可见:采用流式细胞仪分离的性控冻精与新鲜精液相比,对ICSI后的受精率和囊胚率无显著影响;精子活动力和卵子质量对ICSI的效果均有一定程度影响,且随精子活动力的降低和卵母细胞质量的下降而逐渐显著. 相似文献
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A decrease of sperm freezability occurred at the K. breeding station, and this situation lasted longer than a year. Out of the 2550 ejaculates taken from 42 breeding bulls within 12 months, 685, i.e. 26.7%, were unfit for use immediately after sperm collection, mostly owing to a low activity of spermatozoa and pathological forms of their motility, and another 469 ejaculates, i. e. 18.3%, were unfit for use after sperm freezing; on the whole, 1154 (i. e. 45.2%) ejaculates had to be excluded. It was revealed by the vital-lethal primuline test that the spermatozoa died quickly after collection. The findings obtained during an electron-microscopic examination of the spermatozoa at the beginning of the process included visible changes in the ultrastructure of the flagellum, particularly its middle piece (deformed shape, incomplete set of axial filaments, vacuolization of the flagellum, abnormal arrangement of the mitochondrial spiral), numerous abnormities of the external cytoplasmic membrane and invagination, vacuolization, and abnormal density of nucleoplasm. The primary changes on the flagella and in the nucleus give evidence that the testicular tissues were altered. The etiological factors behind these processes are believed to include a reduction in the resistance of bulls due to long-lasting consumption of feeds contaminated with the fungus Aspergillus fumigatus, insufficient movement and bad zoo-hygienic practices, all this combined with the secondary action of the infectious germs of Mycoplasma bovigenitalium, which were revealed by cultivation tests in 50% of the ejaculates of the bulls; a positive antibody titre was demonstrated in all bulls. 相似文献
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精子载体转基因法是一种简便、高效、低廉的生产转基因动物的方法。而精子供体猪的合适选择是此方法成功的关键步骤之一。试验结果表明,长白猪和杂种猪精子的质量比大约克猪精子质量要好些。 相似文献
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Flow cytometry of testicular and sperm cells was used to evaluate effects of pre-weaning zeranol implants on spermatogenesis. Forty five Angus-Simmental bulls were randomly assigned to three treatment groups of 15 bulls each: no implant, one implant at 30 d of age and two implants, one at 30 and the second at 120 d of age. Prior to slaughter at approximately 15 mo, semen was collected from 30 bulls, 10 of each group. Following slaughter, testes were weighed, and testicular biopsies and vas deferens sperm obtained from the same 30 bulls. Testicular and sperm cells were stained with acridine orange and measured by flow cytometry. Proportions of testicular haploid, diploid and tetraploid cells were determined by relative amounts of green (DNA) and red (RNA) fluorescence. Treatment of sperm at low pH prior to acridine orange staining potentially induces partial denaturation of DNA, detectable by the metachromatic shift from green (native DNA) to red (single-stranded DNA) fluorescence. The effect of this shift was quantified by alpha-t [alpha t = red/red + green) fluorescence]. Nonimplanted bulls had heavier (P less than .01) testicular weights than treated bulls. The proportion of haploid cells was greater (P less than .02) and diploid cells less (P less than .03) in testes of nonimplanted bulls. Sperm from implanted bulls had altered chromatin structure, indicated by higher (P less than .05) alpha t values. Flow cytometry is an effective means for detecting changes in testicular cell subpopulations and chromatin structure of sperm. 相似文献