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1.
A comparison of marker-assisted and phenotypic selection for high grain protein content in spring wheat 总被引:3,自引:0,他引:3
Wheat grain protein content (GPC) is a primary end-use quality determinant for hard spring wheat (Triticum aestivum L.), and marker-assisted selection (MAS) could help plant breeders to develop high GPC cultivars. Two experiments were conducted using two populations developed by crossing low GPC cultivars (Ember) and (McVey) with (Glupro), which contains a high GPC QTL from Triticum dicoccoides (DIC). In one experiment, MAS and phenotypic selection (PS) were employed to select high GPC genotypes, and the selected genotypes were grown in six North Dakota (ND), USA environments. In a second experiment, molecular markers were used to select BC2F2 plants from each marker class for the DIC allele from each population. These plants were twice self-pollinated to produce BC2F4 plants, which were grown in single ND and Minnesota (MN) environments. Mean GPC was highest among lines using PS at two environments and not significantly different between MAS and PS in the other four environments. Lines presumably homozygous for DIC alleles had significantly higher GPC than their respective low GPC parents. The phenotypic GPC variation explained by the markers (r
2) was 30% at the ND and 15% at the MN environment. The use of PS was as effective as MAS in selecting for high GPC genotypes and more effective in some environments. This likely can be attributed to PS enabling selection for both the major QTL and other genes contributing to GPC. The use of molecular markers might be more advantageous for transferring the high GPC DIC QTL in a backcrossing program during parent development. 相似文献
2.
STMS markers for grain protein content and their validation using near-isogenic lines in bread wheat 总被引:5,自引:0,他引:5
Harjit -Singh M. Prasad R. K. Varshney J. K. Roy H. S. Balyan H. S. Dhaliwal P. K. Gupta 《Plant Breeding》2001,120(4):273-278
The present study, undertaken as a continuation of an earlier study on quantitative trait loci (QTL) analysis of grain protein content (GPC) in bread wheat (Prasad et al. 1999), includes the following: (1) identification of an additional molecular marker associated with GPC; (2) development of near‐isogenic lines (NILs) for high GPC; and (3) the use of three sets of NILs (a total of 10 NILs) to validate the two available markers linked with QTL for GPC. A total of 114 sequence‐tagged microsatellite site (STMS) primer pairs (that were not used in the previous study) were used for detection of polymorphism between the two parents (PH132, with high GPC; WL711, with low GPC) of a mapping population of 100 recombinant inbred lines (RILs). A total of 95 primer pairs gave amplification products, of which only 30 detected reproducible polymorphism between the parental genotypes. Bulked segregant analysis was conducted using these 30 primers on two bulks (each comprising eight RILs) representing the two extremes of the normal distribution. A solitary primer pair (WMC415) showed association with GPC, which was further confirmed through selective genotyping. Subsequently, 100 RILs were genotyped. A single‐marker linear regression analysis showed significant association between the marker WMC415 and GPC, thus identifying a quantitative trait locus (designated as QGpcccsu‐5A1), which explained 6.21% of the variation for GPC among the RILs. The above STMS marker, together with the STMS marker (WMC41) identified earlier, explains approximately 25% of the variation for GPC. In order to conduct validation of the above two available markers, 10 NILs were developed for high GPC using two genotypes (WL711 and HD2329) with low GPC as recipient parents and another two genotypes (PH132 and PH133) with high GPC as donor parents. NIL 2233 (with 11.7% GPC), derived from HD2329, when tried with WMC41 gave a characteristic amplification profile similar to that of its donor parent PH132, and NIL 2215 (with 11.9% GPC) derived from WL711, when tried with WMC415 gave an amplification profile that resembled its donor parent PH133. The remaining eight NILs with high GPC gave patterns similar to those of their corresponding recipient parents with both the markers, suggesting that either the QTL, other than those associated with the above markers, were actually transferred from the donor parents and contributed to high GPC in these NILs or that recombination had occurred between the markers identified and the corresponding QTL. Thus, the marker validation conducted using NILs, while demonstrating the utility of these two microsatellite markers for use in marker‐assisted selection in plant breeding, also suggested that many more QTL exist that would need to be identified using closely linked molecular markers. 相似文献
3.
QTL for rice grain quality based on a DH population derived from parents with similar apparent amylose content 总被引:5,自引:0,他引:5
A doubled haploid (DH)population consisting of 135 lines, derived from an indica (IR64) and a japonica (Azucena) rice with a similar apparent amylose content (AAC), was used to investigate the genetic factors affecting cooking
and eating quality of rice. AAC,gelatinization temperature (GT), gel consistency (GC) and six starch pasting viscosity parameters
were measured for quantitative trait loci (QTL) analysis using 193 molecular markers mapped on the DH population. A total
of 17 QTLs were detected for the 9 traits, with at least one QTL and as many as 3 QTLs for each individual trait. No QTL for
the measured parameters was found at the wx locus,possibly because of the similar AAC between the parents. Several QTLs with important effects on the variations in the
measured parameters were detected in the present study which have not been found in earlier reports based on populations derived
from parents with different AAC and wxgene alleles. Two interesting loci could be deduced from the present study according to the marker order compared with other
genetic linkage maps. A QTL flanked by Amy2A and RG433 on the end of the long arm of chromosome 6, identified for GT, set
back and consistency viscosity, might cover the gene encoding starch branching enzyme I. Similarly, a QTL flanked by RG139
and RZ58on chromosome 2, detected for hot paste viscosity and breakdown viscosity, might cover the gene encoding starch branching
enzyme III. Generally, traits significantly correlated with each other shared identical QTL, but it was not true in some cases.
The fine molecular mechanisms underlying these traits await further elucidation for the improvement of eating and cooking
quality of rice.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
4.
QTL analysis of cooked rice grain elongation, volume expansion, and water absorption using a recombinant inbred population 总被引:7,自引:0,他引:7
The traits of elongation, volume expansion, and water absorption are very important in determining the quality of cooked rice grains. In this study, quantitative trait loci (QTL) analysis of these traits was performed using a recombinant inbred population derived from a cross between two indica cultivars, ‘Zhenshan 97’ and ‘Minghui 63 ,’ which are the parents of the most widely grown hybrid rice in China. Using a linkage map based on 221 molecular marker loci covering a total of 1796 cM, a total of 33 QTLs were identified for the nine traits tested. QTLs were detected on chromosomes 1– 3 , 5– 9 , and 11 , respectively. The QTLs identified included three for cooked rice grain length elongation (chromosomes 2 , 6 , and 11), six for width expansion (chromosomes 1‐ 3 , 6 , 9 , and 11) and two for water absorption (chromosomes 2 and 6). Interestingly, a single QTL located near the wx gene on chromosome 6 seemed to influence all the traits tested for the cooked rice quality. 相似文献
5.
Quantitative trait loci for grain yield and yield components in a cross between a six-rowed and a two-rowed barley 总被引:8,自引:0,他引:8
Summary The positions of quantitative trait loci (QTL) for yield and yield components were estimated using a 85-point linkage map and phenotype data from a F1-derived doubled haploid (DH) population of barley. Yield and its components were recorded in two growing seasons. Highly significant QTL effects were found for all traits at several sites in the genome. A major portion of the QTL was found on chromosome 2. The effect of the alleles in locus v on thousand grain weight and kernels per ear explained 70–80% of the genetic variation in the traits. QTL × year interaction was found for grain yield. Several different QTL were found within the two-rowed DH lines compared to those found in the six-rowed DH lines. Epistasis between locus v and several loci for yield and yield components indicates that genes are expressed differently in the two ear types. This may explain the difficulties of selecting high yielding lines from crosses between two-rowed and six-rowed barley.Abbreviations DH
doubled haploid
- QTL
quantitative trait locus/loci
- RAPD
random amplified polymorphic DNA
- RFLP
restriction fragment length polymorphism
- T. Prentice
Tystofte Prentice
- V. Gold
Vogelsanger Gold 相似文献
6.
Dissection of the genetic architecture for grain quality‐related traits in three RIL populations of maize (Zea mays L.) 下载免费PDF全文
Zhiyong Wang Na Liu Lixia Ku Zhiqiang Tian Yong Shi Shulei Guo Huihui Su Liangkun Zhang Zhenzhen Ren Guohui Li Xiaobo Wang Yuguang Zhu Jianshuang Qi Xin Zhang Yanhui Chen 《Plant Breeding》2016,135(1):38-46
To manipulate the composition of the maize kernel to meet future needs, an understanding of the molecular regulation of kernel quality‐related traits is required. In this study, the quantitative trait loci (QTL) for the concentrations of grain protein, starch and oil were identified using three sets of RIL populations in three environments. The genetic maps and the initial QTL were integrated using meta‐analyses. A total of 38 QTL were identified, including 15 in population 1, 12 in population 2 and 11 in population 3. The individual effects ranged from 2.87% to 13.11% of the phenotypic variation, with seven QTL each contributing over 10%. One common QTL was found for the concentrations of grain protein and starch in bin 3.09 in the three environments and the three RIL populations. Of the 38 initial QTL, 22 were integrated into eight mQTL by meta‐analysis. mQTL3 and mQTL8 of the key mQTL in which the initial QTL displayed R2 > 10% included six and three initial QTL for grain protein and starch concentrations from two or three populations, respectively. These results will provide useful information for marker‐assisted selection to improve the quality of the maize kernel. 相似文献
7.
Pablo F. Roncallo Gerardo L. Cervigni Carlos Jensen Rubén Miranda Alicia D. Carrera Marcelo Helguera Viviana Echenique 《Euphytica》2012,185(1):77-92
The aim of this work was to map quantitative trait loci (QTLs) associated with flour yellow color (Fb*) and yellow pigment
content (YPC) in durum wheat (Triticum turgidum L. var. durum). Additionally, QTLs affecting flour redness (Fa*) and brightness (FL*) color parameters were investigated. A population
of 93 RILs (UC1113 × Kofa) was evaluated in three locations of Argentina over 2 years. High heritability values (>94%) were
obtained for Fb* and YPC, whereas FL* and Fa* showed intermediate to high values. The main QTLs affecting Fb* and YPC overlapped
on chromosome arms 4AL (4AL.2), 6AL (6AL.2), 7AS, 7AL, 7BS (7BS.2) and 7BL (7BL.2). The 7BL.1 QTL included the Psy-B1 locus, but one additional linked QTL was detected. A novel minor QTL located on 7AS affected Fb*, with an epistatic effect
on YPC. An epistatic interaction occurred between the 7AL and 7BL.2 QTLs. The 4AL.2 QTL showed a strong effect on Fb* and
was involved in two digenic epistatic interactions. The 6AL.2 QTL explained most of the variation for Fb* and YPC. The main
QTLs affecting FL* and Fa* were located on 2BS and 7BL, respectively. These results confirm the complex inheritance of flour
color traits and open the possibility of developing perfect markers to improve pasta quality in Argentinean breeding programs. 相似文献
8.
C. A. McCartney D. J. Somers O. Lukow N. Ames J. Noll S. Cloutier D. G. Humphreys B. D. McCallum 《Plant Breeding》2006,125(6):565-575
Wheat grain quality is a complex group of traits of tremendous importance to wheat producers, end‐users and breeders. Quantitative trait locus (QTL) analysis studied the genetics of milling, mixograph, farinograph, baking, starch and noodle colour traits in the spring wheat population RL4452/‘AC Domain’. Forty‐seven traits were measured on the population and 99 QTLs were detected over 18 chromosomes for 41 quality traits. Forty‐four of these QTLs mapped to three major QTL clusters on chromosomes 1B, 4D, and 7D. Fourteen QTLs mapped near Glu‐B1, 20 QTLs mapped near a major plant height QTL on chromosome 4D, and 10 QTLs mapped near a major time to maturity QTL on chromosome 7D. Large QTLs were detected for grain and flour protein content, farinograph absorption, mixograph parameters, and dietary fibre on chromosome 2BS. QTLs for yellow alkaline noodle colour parameter L* mapped to chromosomes 5B and 5D, while the largest QTL for the b* parameter mapped to 7AL. 相似文献
9.
Alleles on the two dwarfing loci on 4B and 4D are main drivers of FHB‐related traits in the Canadian winter wheat population “Vienna” × “25R47” 下载免费PDF全文
Fusarium head blight (FHB), leaf rust and stem rust are among the most destructive wheat diseases. High‐yielding, native disease resistance sources are available in North America. The objective of this study was to map loci associated with FHB traits, leaf rust, stem rust and plant height in a “Vienna”/”25R47” population. DArT markers were used to generate a genetic map, and quantitative trait loci (QTL) analysis was performed by evaluating 113 doubled haploid lines across three environments in Ontario, Canada. FHB resistance QTL were identified on chromosomes 4D, 4B, 2D and 7A, while a QTL for leaf and stem rust resistance was identified on chromosome 1B. The dwarfing alleles of both Rht‐B1 and Rht‐D1 were associated with increased FHB index and DON content. 相似文献
10.
Chunhua Zhao Yinguang Bao Xiuqin Wang Haitao Yu Anming Ding Chunhui Guan Junpeng Cui Yongzhen Wu Han Sun Xingfeng Li Dongfeng Zhai Linzhi Li Honggang Wang Fa Cui 《Euphytica》2018,214(11):209
Flag leaf-related traits (FLRTs) are determinant traits affecting plant architecture and yield potential in wheat (Triticum aestivum L.). In this study, three related recombinant inbred line (RIL) populations with a common female parent were developed to identify quantitative trait loci (QTL) for flag leaf width (FLW), length (FLL), and area (FLA) in four environments. A total of 31 QTL were detected in four environments. Two QTL for FLL on chromosomes 3B and 4A (QFll-3B and QFll-4A) and one for FLW on chromosome 2A (QFlw-2A) were major stable QTL. Ten QTL clusters (C1–C10) simultaneously controlling FLRTs and yield-related traits (YRTs) were identified. To investigate the genetic relationship between FLRTs and YRTs, correlation analysis was conducted. FLRTs were found to be positively correlated with YRTs especially with kernel weight per spike and kernel number per spike in all the three RIL populations and negatively correlated with spike number per plant. Appropriate flag leaf size could benefit the formation of high yield potential. This study laid a genetic foundation for improving yield potential in wheat molecular breeding programs. 相似文献
11.
QTL detection of seven spike-related traits and their genetic correlations in wheat using two related RIL populations 总被引:2,自引:1,他引:1
Fa Cui Anming Ding Jun Li Chunhua Zhao Lin Wang Xiuqin Wang Xiaolei Qi Xingfeng Li Guoyu Li Jurong Gao Honggang Wang 《Euphytica》2012,186(1):177-192
Spike-related traits contribute greatly to grain yield in wheat. To localize wheat chromosomes for factors affecting the seven spike-related traits??i.e., the spike length (SL), the basal sterile spikelet number (BSSN), the top sterile spikelet number (TSSN), the sterile spikelet number in total (SSN), the spikelet number per spike (SPN), the fertile spikelet number (FSN) and the spike density (SD)??two F8:9 recombinant inbred line (RIL) populations were generated. They were derived from crosses between Weimai 8 and Jimai 20 (WJ) and between Weimai 8 and Yannong 19 (WY), comprising 485 and 229 lines, respectively. Combining the two new linkage maps and the phenotypic data collected from the four environments, we conducted quantitative trait locus (QTL) detection for the seven spike-related traits and evaluated their genetic correlations. Up to 190 putative additive QTL for the seven spike-related traits were detected in WJ and WY, distributing across all the 21 wheat chromosomes. Of these, at least nine pairwise QTL were common to the two populations. In addition, 38 QTL showed significance in at least two of the four different environments, and 18 of these were major stable QTL. Thus, they will be of great value for marker assisted selection (MAS) in breeding programs. Though co-located QTL were universal, every trait owned its unique QTL and even two closely related traits were not excluded. The two related populations with a large/moderate population size made the results authentic and accurate. This study will enhance the understanding of the genetic basis of spike-related traits. 相似文献
12.
José Manoel Colombari Filho Marcos Deon Vilela de Resende Orlando Peixoto de Morais Adriano Pereira de Castro Élcio Perpétuo Guimarães José Almeida Pereira Marley Marico Utumi Flávio Breseghello 《Euphytica》2013,190(1):117-129
A durum wheat recombinant inbred line population developed from PDW 233 × Bhalegaon 4 cross was analyzed in five environments to understand the genetic network responsible for test weight (TW), thousand kernel weight (TKW), grain yield (YLD), spike length (SL), spikelets per spike (SPS), kernels per spike (KER) and kernel weight per spike (KWS). Genotype, environment and their interactions were main sources of variance for all the traits. TW and TKW were influenced by 11 main effect QTL and 6 digenic epistatic interactions detected on chromosomes 2A, 2B, 4B and 7A. Grain yield was influenced by three epistatic interactions and five main effect QTL, of which two on chromosome 2A were most consistent. A major QTL for spike length was observed on chromosome 3B. QTL for spike characters were distributed over 9 chromosomes. All the traits showed significant influence of digenic epistasis (QQ) and, to a certain extent, QTL × environment interactions (QQE). Therefore, while breeding for complex traits like kernel characters and grain yield components, these interactions should also be considered important. The consistent QTL on chromosome 2A between the marker interval Xgwm71.2–Xubc835.4 with pleiotropic effect on TW and TKW, may be utilized in early generation selection to improve TW and TKW and thereby the milling potential of the durum wheat. 相似文献
13.
Quantitative trait loci mapping and epistatic analysis for grain yield and yield components using molecular markers with an elite maize hybrid 总被引:6,自引:0,他引:6
Summary The aim of this investigation was to map quantitative trait loci (QTL) associated with grain yield and yield components in maize and to analyze the role of epistasis in controlling these traits. An F2:3 population from an elite hybrid (Zong3 × 87-1) was used to evaluate grain yield and yield components in two locations (Wuhan and Xiangfan, China) using a randomized complete-block design. The mapping population included 266 F2:3 family lines. A genetic linkage map containing 150 simple sequence repeats and 24 restriction fragment length polymorphism markers was constructed, spanning a total of 2531.6 cM with an average interval of 14.5 cM. A logarithm-of-odds threshold of 2.8 was used as the criterion to confirm the presence of one QTL after 1000 permutations. Twenty-nine QTL were detected for four yield traits, with 11 of them detected simultaneously in both locations. Single QTL contribution to phenotypic variations ranged from 3.7% to 16.8%. Additive, partial dominance, dominance, and overdominance effects were all identified for investigated traits. A greater proportion of overdominance effects was always observed for traits that exhibited higher levels of heterosis. At the P ≤ 0.005 level with 1000 random permutations, 175 and 315 significant digenic interactions were detected in two locations for four yield traits using all possible locus pairs of molecular markers. Twenty-four significant digenic interactions were simultaneously detected for four yield traits at both locations. All three possible digenic interaction types were observed for investigated traits. Each of the interactions accounted for only a small proportion of the phenotypic variation, with an average of 4.0% for single interaction. Most interactions (74.9%) occurred among marker loci, in which significant effects were not detected by single-locus analysis. Some QTL (52.2%) detected by single-locus analysis were involved in epistatic interactions. These results demonstrate that digenic interactions at the two-locus level might play an important role in the genetic basis of maize heterosis. 相似文献
14.
Mapping quantitative trait loci associated with grain filling duration and grain number under terminal heat stress in bread wheat (Triticum aestivum L.) 下载免费PDF全文
Davinder Sharma Rajender Singh Jagadish Rane Vijay Kumar Gupta Harohalli Masthigowda Mamrutha Ratan Tiwari 《Plant Breeding》2016,135(5):538-545
Terminal heat stress has the potential negative impact on wheat production across the world, especially in South Asia. Under the threat of terminal heat stress, wheat genotypes with stay green trait would suffer from high temperature stress during their long grain filling duration (GFD). The genotypes with short GFD would be advantageous. To identify quantitative trait loci (QTL) for heat tolerance, a RIL population of K 7903 (heat tolerant) and RAJ 4014 (heat sensitive) wheat genotypes was investigated under timely and late‐sown conditions. Heat susceptibility index of GFD, yield components and traits under late‐sown condition were used as phenotypic data for QTL identification. Stable QTLs associated with these traits were identified on chromosomes 1B, 2B, 3B, 5A and 6B. The LOD value ranged from 2.9 to 5.0 and the corresponding phenotyping variation explained ranged from 12.0–22%. QTL for heat susceptibility index for the grain filling duration were colocalized with QTL for productive tillers under late sown and GFD under late‐sown condition on chromosomes 1B and 5A, respectively. These genomic regions could be exploited for molecular wheat breeding programmes targeting heat tolerance. 相似文献
15.
Hang Liu Jian Ma Yang Tu Jing Zhu Puyang Ding Jiajun Liu Ting Li Yaya Zou Ahsan Habib Yang Mu Huaping Tang Qiantao Jiang Yaxi Liu Guoyue Chen Youliang Zheng Yuming Wei Xiujin Lan 《Plant Breeding》2020,139(2):284-294
Spike density (SD), an important spike morphological trait associated with wheat yield, is the spikelet number per spike (SNS) divided by spike length (SL). In this study, phenotypic data from eight environments were collected and a recombinant inbred line population (RIL) constructed by the wheat line 20828 and the cultivar 'Chuannong16' and a Wheat55K SNP array-based constructed genetic linkage map were used to identify SD quantitative trait locus (QTL). Correlation between SD and other agronomic traits was calculated. Genes associated with plant growth and development for major loci were predicted. The results showed that 24 QTLs associated with SD were detected in eight environments. Among them, three major QTL, namely QSd.sicau-5B.2, QSd.sicau-2D.3 and QSd.sicau-4B.1, explained up to 35.62%, 14.21% and 11.23% of phenotypic variation, respectively. The positive alleles of them were all derived from 'Chuannong16'. The significant relationships between SD and other agronomic traits were detected and discussed. Taken together, the stably expressed SD QTL under different environments identified in this study provided theoretical guidance for further fine mapping and germplasm improvement. 相似文献
16.
Increasing seed oil content is an important breeding goal for Brassica napus L. (B. napus). The identification of quantitative trait loci (QTL) for seed oil content and related traits is important for efficient
selection of B. napus cultivars with high seed oil content. To get better knowledge on these traits, a molecular marker linkage map for B. napus was constructed with a recombinant inbred lines (RIL) population. The length of the map was 1,589 cM with 451 markers distributed
over 25 linkage groups. QTL for seed oil content, seed hull content and seed coat color in three environments were detected
by composite interval mapping (CIM) tests. Eleven QTL accounted for 5.19–13.57% of the variation for seed oil content. Twelve
QTL associated with seed hull content were identified with contribution ranging from 5.80 to 22.71% and four QTL for seed
coat color accounted for 5.23–15.99% of the variation. It is very interesting to found that co-localization between QTL for
the three traits were found on N8. These results indicated the possibility to combine favorable alleles at different QTL to
increase seed oil content, as well as to combine information about the relationship between seed oil content and other traits. 相似文献
17.
Quantitative trait loci mapping of forage stover quality traits in six mapping populations derived from European elite maize germplasm 下载免费PDF全文
Pengfei Leng Milena Ouzunova Matthias Landbeck Gerhard Wenzel Joachim Eder Birte Darnhofer Thomas Lübberstedt 《Plant Breeding》2018,137(2):139-147
Four forage maize stover quality traits were analysed including in vitro digestibility of organic matter (IVDOM), neutral detergent fibre (NDF), water‐soluble carbohydrates (WSC) and digestibility of NDF (DNDF). We mapped quantitative trait loci (QTL) in three DH (doubled haploid) populations (totally 250–720 DH lines): one RIL population (358 lines) and two testcross (TC) populations, based on field phenotyping at multiple locations and years for each. High phenotypic and genotypic correlations were found for all traits and significant (p < .01) at two locations, and NDF was negatively correlated with the other traits. QTL analyses were conducted by composite interval mapping. A total of 33, 23, 32 and 25 QTL were identified for IVDOM, NDF, WSC and DNDF, respectively, with three, four, five and two major QTL for each. Few consistent QTL for IVDOM, WSC and DNDF were detected in more than two populations. This study contributed to the identification of key QTL associated with forage maize digestibility traits and is beneficial for marker‐assisted breeding and fine mapping of candidate genes associated with forage maize quality. 相似文献
18.
The gene pool of Aegilops tauschii, the D-genome donor of common wheat (Triticum aestivum L.), can be easily accessed in wheat breeding, but remains largely unexplored. In our previous studies, many synthetic hexaploid wheat lines were produced through interspecific crosses between the tetraploid wheat cultivar Langdon and various A. tauschii accessions. The synthetic hexaploid wheat lines showed wide variation in many characteristics. To elucidate the genetic basis of variation in flowering-related traits, we analyzed quantitative trait loci (QTL) affecting time to heading, flowering and maturity, and the grain-filling period using four different F2 populations of synthetic hexaploid wheat lines. In total, 10 QTLs located on six D-genome chromosomes (all except 4D) were detected for the analyzed traits. The QTL on 1DL controlling heading time appeared to correspond to a flowering time QTL, previously considered to be an ortholog of Eps-A m 1 which is related to the narrow-sense earliness in einkorn wheat. The 5D QTL for heading time might be a novel locus associated with wheat flowering, while the 2DS QTL appears to be an allelic variant of the photoperiod response locus Ppd-D1. Some of the identified QTLs seemed to be novel loci regulating wheat flowering and maturation, including a QTL controlling the grain filling period on chromosome 3D. The exercise demonstrates that synthetic wheat lines can be useful for the identification of new, agriculturally important loci that can be transferred to, and used for the modification of flowering and grain maturation in hexaploid wheat. 相似文献
19.
Stephen Byrne Emma Guiney Susanne Barth Iain Donnison Luis A. J. Mur Dan Milbourne 《Euphytica》2009,166(1):61-70
Flowering time is a trait which has a major influence on the quality of forage. In addition, flowering and subsequent seed
yields are important traits for seed production by grass breeders. In this study, we have identified quantitative trait loci
(QTL) for flowering time and morphological traits of the flowering head in an F1 mapping population in Lolium perenne L (perennial ryegrass), a number of which have not previously been identified in L. perenne mapping studies. QTL for days to heading (DTH) were mapped in both outdoor and glasshouse experiments, revealing three and
five QTL for DTH which explained 53% and 42% of the total phenotypic variation observed, respectively. Two QTL for DTH were
detected in both environments, although they had contrasting relative magnitudes in each environment. One QTL for spike length
and three QTL for spikelets per spike were also identified explaining, a total of 32 and 33% of the phenotypic variance, respectively.
Furthermore, the QTL for spike length and spikelets per spike generally coincided with QTL for days to heading, implying co-ordinate
regulation by underlying genes. Of particular interest was a region harbouring overlapping QTL for days to heading, spike
length and spikelets per spike on the top of linkage group 4, containing the major QTL for spike length identified in this
population. 相似文献
20.
QTL detection of seven quality traits in wheat using two related recombinant inbred line populations 总被引:1,自引:0,他引:1
Jun Li Fa Cui An-ming Ding Chun-hua Zhao Xiu-qin Wang Lin Wang Yin-guang Bao Xiao-lei Qi Xing-feng Li Ju-rong Gao De-shun Feng Hong-gang Wang 《Euphytica》2012,183(2):207-226
Grain protein content (GPC) and gluten quality are the most important factors determining the end-use quality of wheat for pasta-making. Both GPC and gluten quality are considered to be polygenic traits influenced by environmental factors and other agricultural practices. Two related F8:9 recombinant inbred line (RIL) populations were generated to localise genetic factors controlling seven quality traits: GPC, wet gluten content (WGC), flour whiteness (FW), kernel hardness (KH), water absorption (Abs), dough development time (DDT) and dough stability time (DST). These lines were derived by crossing Weimai 8 and Jimai 20 (WJ) and by crossing Weimai 8 and Yannong 19 (WY). In total, WJ comprised 485 lines, while WY comprised 229 lines. Data on these seven quality traits were collected from each line in five different environments. Up to 85 putative QTLs for the seven traits were detected in WJ and 65 putative QTLs were detected in WY. Of these QTLs, 31 QTLs (36.47%) were detected in at least two trials in WJ, while 24 QTLs (36.92%) were detected in at least two trials in WY. Three QTLs from WJ and 25 from WY accounted for more than 10% of the phenotypic variance. The total 150 QTLs were spread throughout all 21 wheat chromosomes. Of these, at least thirteen pairwise were common to both populations, accounting for 20.00 and 15.29% of the total QTLs in WJ and WY, respectively. A major QTL for GPC, accounting for 53.04% of the phenotypic variation, was detected on chromosome 5A. A major QTL for WGC also shared this interval, explained more than 36% of the phenotypic variation, and was significant in two environments. Though co-located QTLs were common, every trait had its unique control mechanism, even for two closely related traits. Due to the different sizes of the two line populations, we also assessed the effects of population size on the efficiency and precision of QTL detection. In sum, this study will enhance our understanding of the genetic basis of these seven pivotal quality traits and facilitate the breeding of improved wheat varieties. 相似文献