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1.
天津口岸在日本牡丹苗中截获穿刺短体线虫   总被引:1,自引:0,他引:1  
马以桂  刘鹏  崔铁军 《植物检疫》2003,17(4):197-197
20 0 2年 1 2月 6~ 9日 ,天津出入境检验检疫局对来自日本的牡丹苗 (Paeoniasp .)实施检疫时 ,截获了进境植物检疫潜在危险性线虫———穿刺短体线虫 (Pratylenchuspenetrans)。检验检疫人员剪取牡丹苗根须约 1 0 0 g ,使用改进的贝曼漏斗法对上述植物材料进行分离检验 ,鉴定出穿刺短体线虫。同时还发现了滑刃线虫 (Aphelenchoidessp .)、丝尾垫刃线虫(Filenchussp .)、矛线科线虫、茎线虫 (Ditylenchussp .)、Aphelenchoidesindicus和小杆线虫。穿刺短体线虫属世界性分布 ,在美国东北部危害严重 ,该线虫寄主范围广 ,报道有 3 50多种寄…  相似文献   

2.
莲潜根线虫(Hirschmanniella diversa)属短体线虫科(Pratylenchidea)潜根线虫属(Hirshmanniella)。2006年,连云港出入境检验检疫局在对出口美国的观赏莲藕进行检疫的过程中,发现大量的活体线虫,经鉴定为Hirschmanniella diversa。  相似文献   

3.
2008~2011年,广东局检验检疫技术中心从广州白云机场局、顺德局、高明局、佛山局等口岸送检的样品中检出鳞球茎茎线虫(Ditylenchus dipsac)(3批)、香蕉穿孔线虫(Radopholus simili;)(2批)、短颈剑线虫(Xiphinema brevicollus)(4批)、胼胝拟毛刺线虫(Paratrichodorus porosus)(1批)、最短尾短体线虫(Pratylen-  相似文献   

4.
本文汇总了2008~2010年南海口岸进境林木种苗的线虫截获情况,共截获15科26属超过40种植物寄生线虫,其中检疫性线虫有7种,分别为短体线虫(非中国种)Pratylenchus Filipjev(non-Chinese species)、毛刺线虫属(传毒种类)Trichodorus Cobb(The species transmit viruses)、根结线虫(非中国种)Meloidogyne Goeldi(non-Chinese species)等;根据携带线虫状况分析了检疫性线虫的截获情况,提出了防止危险性线虫入境、扩散措施,为口岸防止线虫入境提供借鉴。  相似文献   

5.
四种短体线虫的形态和分子生物学鉴定   总被引:1,自引:0,他引:1  
 采用形态学和分子生物学方法对来自荷兰的藏橐吾和铃兰、泰国的高山榕、缅甸的香蕉等种苗(球)分别鉴定出玻利维亚短体线虫Pratylenchus bolivianus、铃兰短体线虫P. convallariae、咖啡短体线虫P. coffeae和斯佩奇短体线虫 P. speijeri。对这4种线虫的形态特征进行较为详细的描述后,认为头环、口针、侧区、生殖系统和尾形等形态特征是种类鉴定的重要依据;进一步利用引物28S-D2A/28S-D3Br扩增测序得到上述4种线虫的28S rRNA基因D2/D3区序列,序列分析发现玻利维亚短体线虫从欧洲到美洲的不同种群之间的遗传距离变异较小,仅为0~0.007;铃兰短体线虫同一种群不同个体之间的遗传变异较大,为0.006~0.029;咖啡短体线虫不同种群之间的平均遗传距离为0.013;斯佩奇短体线虫不同种群之间的平均遗传距离为0.010;咖啡短体线虫P. coffeae和斯佩奇短体线虫P. speijeri亲缘关系很近,二者种间平均遗传距离仅为0.026。本研究再次证明线虫28S rRNA基因D2/D3区基因序列可作为短体线虫种间鉴定的依据。  相似文献   

6.
四种短体线虫的形态和分子生物学鉴定   总被引:1,自引:0,他引:1  
 采用形态学和分子生物学方法对来自荷兰的藏橐吾和铃兰、泰国的高山榕、缅甸的香蕉等种苗(球)分别鉴定出玻利维亚短体线虫Pratylenchus bolivianus、铃兰短体线虫P. convallariae、咖啡短体线虫P. coffeae和斯佩奇短体线虫 P. speijeri。对这4种线虫的形态特征进行较为详细的描述后,认为头环、口针、侧区、生殖系统和尾形等形态特征是种类鉴定的重要依据;进一步利用引物28S-D2A/28S-D3Br扩增测序得到上述4种线虫的28S rRNA基因D2/D3区序列,序列分析发现玻利维亚短体线虫从欧洲到美洲的不同种群之间的遗传距离变异较小,仅为0~0.007;铃兰短体线虫同一种群不同个体之间的遗传变异较大,为0.006~0.029;咖啡短体线虫不同种群之间的平均遗传距离为0.013;斯佩奇短体线虫不同种群之间的平均遗传距离为0.010;咖啡短体线虫P. coffeae和斯佩奇短体线虫P. speijeri亲缘关系很近,二者种间平均遗传距离仅为0.026。本研究再次证明线虫28S rRNA基因D2/D3区基因序列可作为短体线虫种间鉴定的依据。  相似文献   

7.
荷兰进境肖竹芋中香蕉穿孔线虫的检疫   总被引:3,自引:3,他引:3  
2003年6月下旬,广州出入境检验检疫局检疫人员在白云国际机场现场随机抽取了进口的荷兰产肖竹芋苗(Calathea)部分样品回实验室检验,在检验过程中,采用浅盘法分离了植物根系和栽培介质中的线虫,在检获的线虫中发现有一些短体科寄生线虫,该线虫经我们对其形态特征进行细致的观察,鉴定为我国一类禁止进境检疫性线虫-香蕉穿孔线虫(Radopholus similis Thorne,1949).  相似文献   

8.
 短体属线虫(Pratylenchus Flipjev,1936)又称根腐线虫,是一类重要的迁移性植物内寄生线虫,世界范围内分布广泛且危害性大,在我国多个省份皆有发生危害。本文采用改进的贝尔曼漏斗法从河南省商丘市、荥阳市和洛阳市的3个玉米土壤样品中分离出根腐线虫,利用光学显微镜进行形态学鉴定,基于rDNA的 ITS和28S D2-D3区序列以及mtDNA-COI序列对供试根腐线虫进行了分子鉴定。形态学和分子生物学鉴定结果均表明来源于商丘样品的SY-1和SY-2种群分别为咖啡短体线虫(P. coffeae)和斯克里布纳短体线虫(P. scribneri);来源于荥阳样品的XY-1种群和洛阳样品的XC-250种群,均为斯克里布纳短体线虫(P. scribneri)。基于rDNA-ITS、28S D2-D3区和mtDNA-COI序列构建的系统进化树结果与已知鉴定结果相一致,本研究鉴定的短体线虫种类,分别单独聚类在各自分支。本研究明确了河南省玉米田3个病样品的病原种类,发现采自商丘的病样品存在两种根腐线虫复合侵染的现象,且3个病样品均发现斯克里布纳短体线虫(P. scribneri)的存在,研究结果为玉米根腐线虫病害的识别与防控提供了一定的科学依据。  相似文献   

9.
日本盆景中截获的短体线虫与根结线虫的鉴定   总被引:1,自引:0,他引:1  
2014年,天津口岸从日本进境盆景中截获3种短体线虫和1种根结线虫。通过形态学和DNA条形码方法将截获的短体线虫鉴定为朱顶红短体线虫(Pratylenchus hippeastri)、伤残短体线虫(P.vulnus)和穿刺短体线虫(P.penetrans)。根据形态学并结合特异PCR和DNA条形码方法,将截获的根结线虫2龄幼虫鉴定为南方根结线虫(Meloidogyne incognita)。朱顶红短体线虫是短体线虫属非中国种,为我国进境植物检疫性有害生物,在挪威槭和榉树盆景中截获该线虫为国内外首次报道。  相似文献   

10.
从加拿大马铃薯种薯中截获植物寄生线虫   总被引:1,自引:0,他引:1  
姜丽 《植物检疫》2001,15(3):162-163
20 0 0年 6月 ,对来自加拿大非疫区的马铃薯种薯实施检疫。在入境检疫中 ,从进境的 3个品种马铃薯种薯中共截获短体线虫属Pratylenchus、根结线虫属Meloidogyne、茎线虫属Ditylenchus、滑刃线虫属Aphelen choides、真滑刃线虫属Aphelenchus、丝尾线虫属Filenchus、垫刃线虫属Tylenchus类线虫 ,其中短体线虫、根结线虫是重要的植物寄生线虫。1 现场检疫对进境的 3个品种每个品种按 2 0 %开袋检查 ,并将种薯分别倒在塑料布上检查袋内是否带有土壤、植物的根、…  相似文献   

11.
Fifteen species of dermestid beetles were recorded at ‘Evolution Canyon’ (EC), Lower Nahal Oren, Mt. Carmel, Israel. They represent ~35% of known Israeli dermestid species. The following three species were recorded for the first time in Israel:Trogoderma svriaca Dalla Torre, 1911;Ctesias svriaca Ganglbauer, 1904; andAnthrenus (s.str.) jordaniens Pic, 1934. Adults of 13 species were collected on the more solar radiated, warmer and climatically more fluctuating south-facing slope (SFS); ten species were collected on the opposite, north-facing slope (NFS), which was cooler and climatically more stable. The abundance of adult dermestid beetles was 1.9 times higher on the SFS than on the NFS (86 and 47, respectively). Species richness and abundance distribution at EC (three collecting stations on each slope and one at the valley bottom) were significantly negatively correlated with the plant cover that consisted of trees and bushes (Spearmanr s ,P=0.007 and 0.039, respectively) and perennials (Spearmanr s ,P=0.039 and 0.077, respectively), indicating that non-woody plants were preferred by adult dermestid beetles.  相似文献   

12.
We first discuss the diversity of fruit fly (Diptera: Tephritidae) parasitoids (Hymenoptera) of the Neotropics. Even though the emphasis is on Anastrepha parasitoids, we also review all the information available on parasitoids attacking flies in the genera Ceratitis, Rhagoletis, Rhagoletotrypeta, Toxotrypana and Zonosemata. We center our analysis in parasitoid guilds, parasitoid assemblage size and fly host profiles. We also discuss distribution patterns and the taxonomic status of all known Anastrepha parasitoids. We follow by providing a historical overview of biological control of pestiferous tephritids in Latin American and Florida (U.S.A.) and by analyzing the success or failure of classical and augmentative biological control programs implemented to date in these regions. We also discuss the lack of success of introductions of exotic fruit fly parasitoids in various Latin American countries. We finish by discussing the most pressing needs related to fruit fly biological control (classical, augmentative, and conservation modalities) in areas of the Neotropics where fruit fly populations severely restrict the development of commercial fruit growing. We also address the need for much more intensive research on the bioecology of native fruit fly parasitoids.  相似文献   

13.
Recent data on the epidemiology of the common mycotoxigenic species of Fusarium, Alternaria, Aspergillus and Penicillium in infected or colonized plants, and in stored or processed plant products from the Mediterranean area are reviewed. Emphasis is placed on the toxigenicity of the causal fungal species and the natural occurrence of well known mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, zearalenone, patulin, Alternaria-toxins and moniliformin), as well as some more recently described compounds (fusaproliferin, beauvericin) whose toxigenic potential is not yet well understood. Several Fusarium species reported from throughout the Mediterranean area are responsible of the formation of mycotoxins in infected plants and in plant products, including: Fusarium graminearum, F. culmorum, F. cerealis, F. avenaceum, F. sporotrichioides and F. poae, which produce deoxynivalenol, nivalenol, fusarenone, zearalenone, moniliformin, and T-2 toxin derivatives in wheat and other small grains affected by head blight or scab, and in maize affected by red ear rot. Moreover, strains of F. verticillioides, F. proliferatum, and F. subglutinans, that form fumonisins, beauvericin, fusaproliferin, and moniliformin, are commonly associated with maize affected by ear rot. Fumonisins, were also associated with Fusarium crown and root rot of asparagus and Fusarium endosepsis of figs, caused primarily by F. proliferatum. Toxigenic A. alternata strains and associated tenuazonic acid and alternariols were commonly found in black mould of tomato, black rot of olive and citrus, black point of small cereals, and black mould of several vegetables. Toxigenic strains of A. carbonarius and ochratoxin A were often found associated with black rot of grapes, whereas toxigenic strains of A. flavus and/or P. verrucosum, forming aflatoxins and ochratoxin A, respectively, were found in moulded plant products from small cereals, peanuts, figs, pea, oilseed rape, sunflower seeds, sesame seeds, pistachios, and almonds. Finally, toxigenic strains of P. expansum and patulin were frequently found in apple, pear and other fresh fruits affected by blue mould rot, as well as in derived juices and jams.  相似文献   

14.
Molecular diagnostic techniques have been developed to differentiate the Ascochyta pathogens that infect cool season food and feed legumes, as well as to improve the sensitivity of detecting latent infection in plant tissues. A seed sampling technique was developed to detect a 1% level of infection by Ascochyta rabiei in commercial chickpea seed. The Ascochyta pathogens were shown to be genetically diverse in countries where the pathogen and host have coexisted for a long time. However, where the pathogen was recently introduced, such as A. rabiei to Australia, the level of diversity remained relatively low, even as the pathogen spread to all chickpea-growing areas. Pathogenic variability of A. rabiei and Ascochyta pinodes pathogens in chickpea and field pea respectively, appears to be quantitative, where measures of disease severity were based on aggressiveness (quantitative level of infection) rather than on true qualitative virulence. In contrast, qualitative differences in pathogenicity in lentil and faba bean genotypes indicated the existence of pathotypes of Ascochyta lentis and Ascochyta fabae. Therefore, reports of pathotype discrimination based on quantitative differences in pathogenicity in a set of specific genotypes is questionable for several of the ascochyta-legume pathosystems such as A. rabiei and A. pinodes. This is not surprising since host resistance to these pathogens has been reported to be mainly quantitative, making it difficult for the pathogen to overcome specific resistance genes and form pathotypes. For robust pathogenicity assessment, there needs to be consistency in selection of differential host genotypes, screening conditions and disease evaluation techniques for each of the Ascochyta sp. in legume-growing countries throughout the world. Nevertheless, knowledge of pathotype diversity and aggressiveness within populations is important in the selection of resistant genotypes.  相似文献   

15.
《干旱区科学》2014,(6):782-782
正Journal of Arid Land(JAL)is an international journal(ISSN 1674-6767;CN 65-1278/K)for the natural sciences,sponsored by the Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences and Science Press.It is published by Science Press and Springer-Verlag Berlin Heidelberg bimonthly.JAL publishes original,innovative,and integrative research from arid and semiarid regions,ad  相似文献   

16.
The genera ofMicrogaster Latreille 1804 andHygroplitis Thomson 1895 from China are presented systematically in this paper. Thirty-two species ofMicrogaster and three species ofHygroplitis are known in China. Diagnosis, character variation, distribution and host of each species among the two genera are presented, including its host and distribution. Keys to the species ofMicrogaster andHygroplitis are given. http://www.phytoparasitica.org posting Dec. 19, 2006.  相似文献   

17.
Plant Viruses Transmitted by Whiteflies   总被引:18,自引:0,他引:18  
One-hundred and fourteen virus species are transmitted by whiteflies (family Aleyrodidae). Bemisia tabaci transmits 111 of these species while Trialeurodes vaporariorum and T. abutilonia transmit three species each. B. tabaci and T. vaporariorum are present in the European–Mediterranean region, though the former is restricted in its distribution. Of the whitefly-transmitted virus species, 90% belong to the Begomovirus genus, 6% to the Crinivirus genus and the remaining 4% are in the Closterovirus, Ipomovirus or Carlavirus genera. Other named, whitefly-transmitted viruses that have not yet been ranked as species are also documented. The names, abbreviations and synonyms of the whitefly-transmitted viruses are presented in tabulated form together with details of their whitefly vectors, natural hosts and distribution. Entries are also annotated with references. Whitefly-transmitted viruses affecting plants in the European–Mediterranean region have been highlighted in the text.  相似文献   

18.
Liriomyza cicerina (Diptera: Agromyzidae) is an important pest on chickpea in Turkey. The objective of this study was to determine the parasitoids and rates of parasitism ofL. cicerina on chickpea (Cicer arietinum L.) during the 2005 and 2006 seasons in ?anl?urfa province, Turkey. Leaves with mines were sampled weekly and kept in the laboratory to observe and count emerging leafminer and parasitoid adults. Eight parasitoid species were collected: the braconidsOpius monilicornis Fischer andOpius tersus Foerster and the eulophidsDiaulinopsis arenaria (Erdös) andNeochrysocharis formosa (Westwood), which occurred in both the winter and summer seasons;Diglyphus crassinervis Erdös,Neochrysocharis ambitiosa Hansson,Neochrysocharis sericea (Erdös) andPediobius metallicus (Nees), which occurred only in the summer growing areas.Diaulinopsis arenaria was the predominant parasitoid with 4–7.7% parasitism rate whileN. ambitiosa andO. monilicornis were the second and third most predominant species. The results of these trials show that sinceDia. arenaria occurred throughout every season, it could potentially be used for control of the leafminerL. cicerina.  相似文献   

19.
Systematic information on the quantitative impact of Z ygogramma bicolorata on the biology of P arthenium hysterophorus is crucial as the seeds of this weed continue to germinate from the accumulated soil seed bank throughout the year in the form of different germinating flushes, while the activity of the beetle ceases during winter as it enters diapause. Therefore, plant–herbivore interactions need to be explored to develop predictions of the overall impact of the introduced beetle on the weed. The findings revealed that defoliation by Z . bicolorata had a significant impact on the plant height, density and flower production in flushes F 3, F 4 and F 5, but not in F 1 and F 2 that exhibited longer periodicity, profuse branching, a longer flowering period and maximum flower production and contributed mostly to the existing seed soil bank. Therefore, total depletion of the existing soil seed bank was not possible. Consequently, the effect of augmentative field releases of laboratory‐reared beetles was explored on F 1 and F 2 in February for three consecutive years (2011–2013). Before initiating the trial, random soil samples were taken from the plots that were assigned to the paired treatments (i.e. with the beetle and without the beetle [insecticide‐treated]) and it was found that the seed bank in those samples did not differ. The single release of Z . bicolorata adults at five per plant at the six‐leaf stage significantly reduced the soil seed bank, compared to without the biocontrol agent, irrespective of the flushes at the end of the season.  相似文献   

20.
Solacol®, a formulation of the antibiotic validamycin, at 0.33% in 2% malt extract agar, reduced the spread of fungi on dilution plates drastically and allowed twice as much incubation time before subculturing; this resulted in an elevated number of species isolated. Using pure cultures of 62 common soil fungi, it was shown that all fast-growing species (exceptPythium ultimum) were efficiently inhibited but not completely suppressed. Inhibition was comparable to that by 0.5% oxgall, though, while this substance completely suppressed several species, Solacol very strongly inhibited onlyGaeumannomyces graminis, Gerlachia nivalis, Harzia acremonioides, Verticillium biguttatum andRhizoctonia solani. In a further experiment each separate constituent of Solacol was tested against 22 fungi at equivalent concentrations. Validamycin strongly inhibitedChaetomium globosum and two Basidiomycetes, though hardly more than the non-ionic detergent which mainly inhibited the other fungi. A few species were, however, more inhibited by Solacol than by the detergent alone. Solacol at 0.33% is a suitable aid in dilution plating of soil fungi, by increasing the number of colonies and species observed.Samenvatting Solacol®, een formulering van het antibioticum validamycine, remde de groei van schimmels in verdunningsplaten met een concentratie van 0.33% in 2% moutagar en maakte het mogelijk de periode tot afenten met een factor 2 te verlengen; daardoor was het aantal geïsoleerde soorten duidelijk toegenomen. Met reincultures van 62 algemene grondschimmelsoorten werd aangetoond, dat alle snelgroeiende soorten (met uitzondering vanPythium ultimum) voldoende geremd, maar niet volkomen onderdrukt werden. Het remmingspercentage was vergelijkbaar met dat van 0.5% ossegal, hoewel dit laatste sommige soorten volkomen onderdrukte; Solacol remde alleenGaeumannomyces graminis, Gerlachia nivalis, Harzia acremonioides, Verticillium biguttatum enRhizoctonia solani zeer sterk. In een volgend experiment werden de componenten van Solacol t.o.v. 22 fungi apart getoetst in concentraties equivalent aan 0.33% Solacol. Validamycine remde alleenChaetomium globosum en twee basidiomyceten behoorlijk, maar nauwelijks meer dan de niet-ionische uitvloeier, die in hoofdzaak de overige remeffecten veroorzaakte. Enkele soorten werden echter door het complete Solacol veel sterker geremd dan door de uitvloeier alleen. Solacol in een verdunning van 0,33% wordt aanbevolen bij verdunningsplaten voor het isoleren van grondschimmels ten einde het aantal kolonies en soorten te verhogen.  相似文献   

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