共查询到20条相似文献，搜索用时 15 毫秒
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AIM: To observe the effects of exercise (EX) and forsamax (FOX) on bone mineral density (BMD) and nerve conduction in ovariectomized rats, and to determine whether exercise can enhance the effects of forsamax on postmenopausal osteoporosis (PMOP). METHODS: Ninety 6-month-old female SD rats were randomly divided into 2 groups: sham group (18 rats) and ovariectomized group (72 rats). Eight weeks after ovariectomy, dual-energy X-ray absorptiometry was applied to scan the BMD of the fourth lumbar vertebra. The level of serum estradiol (E2) was analyzed by ELISA. The living ovariectomized rats were then divided into 4 groups: ovariectomized model group (OVX), fosamax treatment group (OVX+FOX), exercise treatment group (OVX+EX), and exercise combined with fosamax treatment group (OVX+FOX+EX). After treated with fosamax (1 mg/kg intragastrically) and/or exercise for 12 weeks, the BMD of the fourth lumbar vertebra, the motor nerve conduction velocity (MCV), motor distal latency (ML) and compound muscle action potential (CMAP) of the left femoral nerve were detected. The levels of serum type 1 procollagen carboxy-terminal propeptide (PICP) and type I collagen carboxy-terminal cross-linked telopeptide (ICTP) were also analyzed by ELISA. RESULTS: Twelve weeks after FOX and/or EX treatment, ovariectomized rats showed obviously lower BMD, higher PICP and ICTP than those in sham group (P<0.05). FOX or EX significantly increased BMD and reduced PICP induced by ovariectomy (P<0.05). FOX significantly reduced ICTP (P<0.05), but no remarkable difference was observed in OVX+FOX group as compared with OVX group. EX+FOX significantly increased BMD and reduced PICP and ICTP compared with FOX or EX alone (P<0.05). However, no obvious difference was observed between OVX+FOX group and OVX+EX group. No distinct difference in MCV and CMAP among the 5 groups was found. Neither ovariectomy nor FOX significantly affected ML. EX or EX+FOX made ML shorter than that in OVX group (fP<0.05), and ML was remarkably shortened in OVX+FOX+EX group than that in FOX alone group on the left femoral nerve (P<0.05). No significant difference between FOX and EX in the protective effects against ovariectomized rats was observed. CONCLUSION: Exercise and fosamax may restrain bone absorption effect of osteoclast and then improve the bone mineral density in ovariectomized rats. 相似文献
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AIM:To investigate the prevalence of vitamin D receptor (VDR) gene polymorphism in Guangzhou postmenopausal women and to study the relationship between bone mineral density (BMD) and VDR gene polymorphism.METHODS:The genotype of VDR gene of 203 postmenopausal women in Guangzhou was detected by polymerase chain reaction-restriction fragment length polymorphism. BMD of lumbar spine, femoral neck, troch and Wards triangle were measured by dual-energy X-ray absorptiometry. RESULTS:The distribution of VDR in 203 subjects was BB genotype 17(8.3%), Bb 60(29.6%), bb(62.1%), respectively. The B allelic gene frequencies reached 23.05%. The distribution followed the Hardy-Weinberg equilibrium. The difference was found in lumbar spine BMD between bb and the other two genotypes (P<0.05), but no significant difference between Bb and BB genotype (P>0.05). There was no significant difference in BMD of the other region among three genotypes (P>0.05). CONCLUSION:Genotype of VDR is related to BMD, but there is no enough evidence to support genotype of VDR as a genetic marker in predicting the risk of developing osteoporosis in Guangzhou postmenopausal women. 相似文献
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LIN Xing-guang ZHOU Qi WANG Li ZHANG Wei-na LUO Zhen-long CHEN Zhong-hua CHANG Sheng 《园艺学报》2010,26(8):1604-1609
AIM: To investigate the effect of estrogen(E2) and progesterone(P4) alone or applied together to H-Y skin graft and the potential mechanism.METHODS: The female C57BL/6 mice were ovariectomized and divided into four groups(n=12 in each). The mice were treated consecutively for 14 d with subcutaneous injection of saline, E2 and P4 alone or in combination, respectively. Before and after H-Y skin grafting, half mice of each group were sacrificed, and the CD4+CD25+Foxp3+ regulatory T cells of peripheral blood and the serum cytokines were detected by flow cytometry and ELISA, respectively. The skin graft survivals of the other half were observed.RESULTS: E2 alone could significantly augment the proportion of regulatory T cells. In the presence of H-Y antigen, this effect was further enhanced(P<0.05). By contrast, P4 had no effect on the expression of Foxp3, regardless of the presence of H-Y antigen or not(P>0.05). The effect of E2 in combination with P4 was similar to that of E2 alone(P>0.05). The administration of sex hormone regardless of E2 and P4 alone or in combination, significantly decreased production of pro-inflammatory cytokines, but increased production of anti-inflammatory cytokines(P<0.05). The skin graft survivals were significantly prolonged in the different experimental groups compared to vehicle control group. E2 and P4 had a synergistic effect to prolong the skin graft survivals(P<0.05). CONCLUSION: E2 and P4 suppress the inflammatory response and enhance the regulatory response to exogenous antigen, through influencing the levels of cytokines and/or the proportion of regulatory T cells, which may contribute to induce the transplant tolerance. 相似文献
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AIM:To investigate the effect of 17β-estradiol (E2) on myocardial hypertrophy induced by endothelin-1(ET-1) and the related mechanism. METHODS:Myocardial cells from neonate rats were cultured in vitro and myocardial hypertrophy model was established with ET-1.The effects of 17β-estradiol on myocardial hypertrophy were observed. The role of ERK1/2 in the effects of 17β-estradiol was also detected. RESULTS:Compared with control group,ET-1 increased cell protein content,cell surface area and ［3H］-Leucine ［3H］-Leu) incorporation. Pretreatment with E2 for 24 h could inhibit the increase in cell protein content,cell surface area and ［3H］-Leu incorporation induced by ET-1.ET-1 significantly stimulated ERK1/2 activity,which was prevented by pretreatment with E2.Tamoxifen,estradiol receptor antagonist,partially inhibited the effect of E2. The ability of ET-1 to stimulate ［3H］-Leu incorporation was significantly blocked by PD98059,which could enhance the inhibitory effect of E2 on the increase of ［3H］-Leu incorporation in cardiomyocytes induced by ET-1.CONCLUSION:E2 can inhibit cardiomyocyte hypertrophy induced by ET-1. This effect is mediated by estrogen receptor. ERK1/2 signal pathway is closely correlated with the inhibitory effect of E2 on cardiomyocyte hypertrophy induced by ET-1. 相似文献
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AIM: To observe the therapeutic effect of modified Bushen Huoxue granules (MBHG) on ovariectomized osteoporosis rats. METHODS: Female SD rats were randomly given sham operation (n=10) and ovariectomy, and then the model rats were further randomly divided into model group, MBHG treatment groups at doses of 200 mg/kg, 100 mg/kg and 50 mg/kg respectively, and positive control (estradiol valerate) group, with 10 rats in each group by intragastric administration for 12 weeks. The morphology, area, thickness, spacing and area percentage of trabecular bone in the rats were observed. The serum levels of calcium (Ca), phosphorus (P) and alkaline phosphatase (ALP) were mea-sured by automatic analyzer. Bone mineral density (BMD) was analyzed. Serum estradiol (E2), osteocalcin (BGP), osteoprotegerin (OPG), receptor activator of nuclear factor-κB (RANK) and receptor activator of nuclear factor κB ligand (RANKL) levels were detected by ELISA. RESULTS: Compared with model group, trabecular bone significantly widened in all treatment groups with large number, and net-like structure restored partially. The thickness, area and area percentages of trabecular bone in treatments groups were higher than those in model group,and trabecular spacing was less than that in model group (P<0.05). The serum Ca, P, E2 and OPG, and femoral BMD were significantly higher in treatment groups than those in model group, and the levels of ALP, BGP, RANK and RANKL were significantly lower than those in model group (P<0.01).CONCLUSION: MBHG has a significant therapeutic effect on ovariectomized osteoporosis rats. The mechanism may be related to the regulation of OPG and inhibition of RANKL secretion. 相似文献
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AIM: To conduct the relevance analysis of serum Dickkopf-1 (Dkk-1) and bone mineral density (BMD) in the different ages of female populations. METHODS: The women volunteers (n=100, 20~80 years old) were selected and divided into young group (20~39 years old), middle age group (40~59 years old) and elderly group (60~80 years old). The serum levels of Dkk-1 in the 3 groups of volunteers were detected by ELISA. In the middle age group, 25 people of 45~55 years old were selected and divided into postmenopausal group and premenopausal group to detect the serum level of Dkk-1 in the 2 groups of volunteers by ELISA. The BMD was measured by the method of dual energy X-ray absorptiometry. The differences of Dkk-1 expression levels among different ages of female populations, and the relevance with BMD were compared. RESULTS: With the increase in age, the serum Dkk-1 expression level increased (P<0.05), and BMD were reduced (P<0.05). The blood level of Dkk-1 and BMD negatively correlated (P<0.05) in the 3 groups of volunteers. The serum levels of Dkk-1 and BMD had stronger negative correlation in postmenopausal women group than that in premenopausal women group. CONCLUSION: With the increase in age, the expression level of serum Dkk-1 increases and the BMD level decreases, which contribute to a risk of osteoporosis. In the same age range, the postmenopausal women express higher level of Dkk-1, and the decreased BMD is more obvious, which contribute to a greater risk of osteoporosis. The increased level of Dkk-1 also inhibits bone formation and promotes bone resorption. It may become a new target for preventing and treating osteoporosis. 相似文献
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AIM:To investigate the effects of phytoestrogen α-zearalanol (ZAL) on hypoxia/reoxygenation (H/R) injury and mechanism involved in human umbilical vein endothelial cells (HUVECs). METHODS:HUVECs were exposed to hypoxia for 3 hours and then reoxygenation 1 hour. ZAL or 17β-estradiol (E2) at concentrations of 10-9-10-6 mol/L were pretreated before hypoxia. The survival rate of HUVECs was detected by MTT. Either the activities of LDH and SOD or the level of MDA in supernatant was detected by spectrophotometry. RESULTS:The survival rate of HUVECs and the activity of SOD were significantly decreased (P<0.01), while the activity of LDH and the level of MDA were significantly increased (P<0.01) after H/R. These changes were reversed by pretreatment with ZAL or E2, and there was no significant difference between their effects in the same dose of ZAL and E2. CONCLUSION:These results suggest that phytoestrogen ZAL protects HUVECs from H/R injury by inhibiting the oxidative stress, which was similar to E2. 相似文献
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AIM: To observe the effect of endoxin antagonist,anti-digoxin antiserum,on endoxin level,ATPase activities,intramitochondrial total calcium concentration and gene expression of sodium pump isoforms in myocardium of rats with myocardial ischemia reperfusion (MIR).METHODS: Fifty-six male Sprauge Dawley rats were randomly divided into 7 groups.Sham operation group: silk suture threading the left anterior descending coronary artery without ligature;MIR group: left anterior descending coronary artery was subjected to 30 min ligation followed by 45 min reperfusion;normal saline group: MIR model was given 5 mL/kg normal saline;verapamil group: MIR model was given 5 mg/kg verapamil;low dose antidigoxin antiserum group: MIR model was given 8.6 mg/kg antidigoxin antiserum;middle dose antidigoxin antiserum group: MIR model was given 17.3 mg/kg antidigoxin antiserum;high dose antidigoxin antiserum group: MIR model was given 34.5 mg/kg antidigoxin antiserum.All drugs were injected into vessel via femoral vein within 5 min before reperfusion,respectively.After reperfusion,left ventricle myocardium samples were processed immediately in order to measure the activity of Na+ K+ATPase and Ca2+Mg2+ATPase,endoxin level,intramitochondrial total calcium concentration and the experssion of α1,α2,α3 and β1 isoforms of sodium pump on mRNA and protein levels by RT-PCR and Western blotting and immunohistochemical assay,respectively.RESULTS: After MIR,the level of endoxin in myocardium was obviously increased.The activities of Ca2+Mg2+ATPase and Ca2+ Mg2+ ATPase in myocardial membrane were significantly decreased while intramitochondrial total calcium concentration increased.The gene expression of the α1,α2,α3 and β1 isoforms of sodium pump at both mRNA and protein levels were reduced markedly.Only the effect of verapamil on reducing intramitochondrial total calcium concentration was observed.Antidigoxin antiserum significantly reduced the level of endoxin in myocardium,restored the activities of Na+ K+ATPase and Ca2+Mg2+ATPase,reduced intramitochondrial total calcium concentration,and up-regulated the expression of α1,α2,α3 and β1 isoforms of sodium pump at both mRNA and protein levels.CONCLUSION: MIR results in increase of endoxin secretion.The latter depresses the activity of Na+ K+ATPase by down-regulating the gene expression of α1,α2,α3 and β1 isoforms of sodium pump in myocardial membrane,and also induces intramitochondrial calcium overload,thereby mediates MIR injury. 相似文献
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AIM: To observe the effects of folic acid (FA) on antioxidant enzyme, nitric oxide synthase (NOS) and nitric oxide (NO) in ovariectomized (OVX) rats.METHODS: Forty three-month-old female SD rats were randomly divided into 5 groups: sham group, OVX group, diethylstilbestrol group (0.03 mg·kg-1·d-1), low-dose FA group (5 mg·kg-1·d-1) and high-dose FA group (20 mg·kg-1·d-1). Gastric gavage started 1 week after operation and lasted for 10 weeks. The rats in sham group and OVX group were given distilled water instead of FA as controls. At the end of the 10th week, the L5 vertebra and right femur were removed for determination of bone mineral density (BMD). The bone homogenates were made using the L3 and L4 vertebrae. The levels of the total antioxidant capacity (TAC), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), NOS and NO were detected in plasma and bone homogenates.RESULTS: Compared with sham group, the BMD levels in L5 vertebra and right femur and the levels of GSH-Px and NO in the plasma were all decreased. The levels of TAC, GSH-Px, NOS and NO in the bone homogenates were also decreased, while the MDA concentration was increased in OVX group (all P < 0.01). Compared with OVX group, the levels of TAC, GSH-Px, NOS, NO and BMD of the L5 vertebra and right femur were all increased, while the MDA concentration was decreased in high-dose FA group (all P < 0.01). CONCLUSION: In female SD rats, ovariectomy leads to a significant reduction of antioxidant enzyme, NOS and NO levels. Oxidative stress is possibly involved in the development of osteoporosis. Protection against osteoporosis by high-dose FA may be linked to improvement of antioxidant enzyme activity, the levels of NOS and NO as well as a reduction of oxidative stress in ovariectomized rats. 相似文献
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AIM: To explore the relationship between the changes of bone mineral density (BMD) and bone marrow angiogenesis in ovariectomized rats. METHODS: Thirty female Sprague-Dawley rats (3 month-age) were randomly divided into ovariectomized (OVX) groups and sham operated (sham) groups, and executed after 4 weeks, 8 weeks and 12 weeks respectively. The bone mineral density (BMD) of left femora was measured. The right distal femoral epiphysis was fixed in formalin, decalcificated by EDTA-Na2, dehydrated and embedded in paraffin. Four-μm-thick slices were obtained from the paraffin section and stained with haematoxylin-eosin (HE) for bone marrow pathological examination. The number of bone marrow microvessels was examined by means of immunohistochemical staining for CD34 to stain the endothelial cells of blood vessels to definite the bone marrow microvascular density (MVD). Plasma levels of vascular endothelial growth factor (VEGF) were examined by the method of ELISA. RESULTS: The BMD of femoral in 8-week OVX group were decreased significantly compared to the 8-week sham group (P<0.05), suggesting the establishment of osteoporosis model. Meanwhile, the area of hematopoietic tissue decreased and the area of adipose tissue increased. These changes became obviously in 12-week OVX group, and the area of trabecular bone and the bone marrow MVD significantly decreased compared to the 12-week sham group. A positive correlation between MVD and BMD, area of hematopoietic or trabecular bone as well as a negative correlation between MVD and area of adipose tissue were observed. The plasma levels of VEGF in OVX group were not significantly different from that in sham group, and had no correlation relationship with the indexes of bone marrow pathology. CONCLUSION: There has an increase in MVD companied with the bone mass loss and hematopoietic tissue decreased in ovarietomized rats, which provide experiment proof to treat osteoporosis with the means of promoting of MVD in bone marrow. 相似文献
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AIM:To study the inhibitory effect of IGF binding protein-4 (IGFBP-4), especially in corporation with heparin on the pathopoiesis of insulin-like growth factor-1 (IGF-1) in alveolitis and fibrosis. METHODS:The diploid human embryonic lung (HEL) fibroblasts were incubated respectively with control, 100 μg/L IGF-1, 100 μg/L IGF-1+100 μg/L IGFBP-4, 100 μg/L IGF-1+200 μg/L IGFBP-4, 100 μg/L IGF-1+100 μg/L IGFBP-4+100 μg/L heparin, 100 μg/L IGF-1 + 100 μg/L IGFBP-4+200 μg/L heparin, 100 μg/L IGF-1+100 μg/L heparin, 100 μg/L IGF-1+200 μg/L heparin for 24 h. Then the content of glucose transporter-4 (GLUT-4), hexokinase-2 (HK-2), collagen-4 and elastin were detected, respectively. RESULTS:Compared with control group, HK-2, GLUT-4, elastin and collagen-4 expressed in IGF-1 group were increased obviously. The expression in the group of IGFBP-4 plus IGF-1 was more than that in IGF-1 group. However, all expression was depressed strikingly when heparin was added. CONCLUSION:(1) IGF-1 apparently stimulates HK-2, GLUT-4, elastin and collagen-IV secretions from lung fibroblasts. (2) The intact IGFBP-4 associated with heparin can inhibit the pathopoiesis of IGF-1. 相似文献
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AIM: To explore the effect and mechanism of liposome prostaglandin E1(Lipo-PGE1) on liver blood perfusion by different time and medication.METHODS: Twelve healthy adult dogs were injected with Lipo-PGE11 μg/kg via left small saphenous vein at speed of 0.05 μg·kg-1·min-1.Liver computed tomography perfusion imaging (CTPI) was performed on 0,5,15 and 30 min,and the value of hepatic arterial perfusion (HAP),portal vein perfusion (PVP) and total liver perfusion (TLP) among groups were compared.The impacts of Lipo-PGE1 on liver haemodynamics at different time were investigated.Twenty-four health dogs were randomly divided into four groups: control group,peripheral vein group,hepatic artery group and superior mesenteric artery group.Liver CTPI was performed at 5 min after 1 μg/kg Lipo-PGE1 administration in those groups.The values of HAP,PVP and TLP were compared and effects of Lipo-PGE1 on liver blood flow by different medication were observed.RESULTS: The values of liver perfusion (mL·min-1·mL-1) at 0,5,15 and 30 min after 1 μg/kg Lipo-PGE1 administration via vein were as follows: HAP: 0.22 ±0.65,0.24±0.65,0.22±0.69,0.22±0.06;PVP: 1.22±0.40,1.88±0.59,1.55±0.55,1.29 ±0.57;TLP: 1.44±0.42,2.12±0.61,1.77±0.56,1.51±0.58,respectively.No significant difference in HAP among groups was observed,but in PVP and TLP,significant differences (F=3.812,P<0.05;F=3.805,P<0.05) among groups were found.The values of PVP and TLP were most obviously increased at 5 min,and the values of PVP and TLP were still on the high level at 15 min and 30 min.The values of liver perfusion (mL·min-1·mL-1) by different medication were as fellows: HAP: 0.22±0.06,0.24±0.06,0.31±0.07,0.26±0.05;PVP: 1.28±0.38,2.33±0.41,2.37±0.55,2.83±0.94;TLP: 1.50±0.40,2.57±0.42,2.67± 0.58,3.09±0.94,respectively.No significant difference in HAP among groups (F=2.248,P>0.05) was found,but in PVP and TLP group,significant differences (F=6.892,P<0.01;F=7.802,P<0.01) among groups were observed.In addition,superior mesenteric artery group showed higher value of PVP and TLP than other methods.CONCLUSION: Lipo-PGE1 obviously increases liver blood perfusion,especially for portal vein perfusion.Interventional technology provides an effective pathway to improve hepatic perfusion. 相似文献
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AIM: To investigate the effects of drynaria total flavonoids on serum levels of leptin (LEP), interleukin 6(IL-6), prostaglandin E2(PGE2) and the expression of bone β2-adrenergic receptor (ADRB2) in a rat model of ovariectomized osteoporosis(OP). METHODS: The osteoporosis model was established by ovariectomy. Twelve weeks after modeling,bone mineral density (BMD) was determined by dual-energy X-ray absorptiometry to verify successful modeling.Enzyme-linked immunosorbent assay was applied to detect the concentrations of LEP, IL-6 and PGE2 in serum. The expression of ADRB2 was determined by immunohistochemical technique. RESULTS: Compared with sham group,BMD of the rats in model group significantly decreased in multiple regions 12 weeks after modeling(P<0.01). The serum levels of LEP, IL-6 and PGE2 in model group were significantly higher than those in sham group(P<0.05). The levels of LEP, IL-6 and PGE2 in drynaria total flavonoids group were significantly lower than those in model group(P<0.01). No significant difference of PGE2 between these 2 groups was observed. The ADRB2 expression in sham group and treatment group was significantly different from that in model group, and no significant difference between sham group and treatment group was found. CONCLUSION: The serum levels of LEP, IL-6 and PGE2 and the expression of bone ADRB2 increased in OP rats.Drynaria total flavonoids reduce the production of LEP, IL-6 and the expression of ADRB2, and suppress the bone absorption, which may be one of the mechanisms in treating OP. 相似文献
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WU Su-hua MA Hong DONG Yu-gang HE Jian-gui LIAO Xin-xue LIU Jun ZENG Wu-tao DU Zhi-min 《园艺学报》2007,23(9):1692-1694
AIM: To evaluate complement activation in patients with all forms of acute coronary syndromes (ACS) and to examine the relationship between the degree of complement activation and myocardial injury.METHODS: The subjects were divided into 2 groups: 110 ACS patients (group ACS) and 18 healthy persons (group control).One hundred and ten patients with ACS were divided into 3 sub-group: 51 patients with ST-segment elevated myocardial infarction (STEMI),28 patients with non-ST-segment elevated myocardial infarction (NSTEMI) and 31 patients with unstable angina (UA).Complement 3 (C3),complement 4 (C4),troponin T (TnT) as well as creatine kinase MB (CK-MB) were evaluated.RESULTS: Plasma C3 and C4 peak levels were significantly higher in patients with STEMI ［(1 525±302)mg/L and (423±123) mg/L］ and NSTEMI ［(1 516±289)mg/L and (396±68) mg/L］ than those in patients with UA ［(1 275±172)mg/L and (356±91) mg/L］ and the control subjects ［(1 072±196)mg/L and (182±73) mg/L］ (P<0.01 for all).Also,C3 and C4 serum levels in patients with UA were significantly higher than those in control subjects (P<0.01 for all).At one-week follow-up,plasma levels of C3 and C4 were significantly different among various days in patients with ACS (P<0.01).Plasma C3 and C4 levels in ACS showed a relationship with peak creatine kinase MB (CK-MB) and troponin T (TnT) levels (P<0.01).CONCLUSION: Plasma C3 and C4 levels are elevated in ACS in present study.The relationship between C3,C4 levels and ACS suggests that the complement activation is related to necrosis within the myocardium. 相似文献
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AIM: To investigate the relationship between serum levels of TNF-α, sTNFRI, IL-6, IL-10, TGF-β1 and cardiac function in elderly patients with heart failure.METHODS: The serum levels of TNF-α, sTNFRI, IL-6, IL-10 and TGF-β1 were determined by ELISA in 112 elderly patients with heart failure and 60 elderly normal subjects.The left ventricular end-diastolic diameter (LVEDD) and left ventricular ejection fraction (LVEF) were measured by color Doppler ultrasonic instrument.RESULTS: (1) Compared with normal subjects group, the serum levels of TNF-α, sTNFRI, IL-6, IL-10 and TGF-β1 in elderly patients with heart failure were significantly elevated (P<0.05 or P<0.01), and increased gradually with upgrade of cardiac function according to cardiac function classification of New York Heart Association (P<0.05 or P<0.01).(2) The ratio of TNFα/sTNFRI and IL-6/IL-10 in patients with heart failure were notably higher than those in control (P<0.05, P<0.01) and increased gradually with upgrade of cardiac function classification (P<0.05, P<0.01).(3) In heart failure group, a significantly positive correlation was found between serum levels of TNF-α, IL-6 and LVEDD.A significantly negative correlation between serum levels of TNF-α, IL-6 and LVEF was also observed.CONCLUSION: The serum levels of pro-inflammatory cytokines are closely related with cardiac function in elderly patients with heart failure.The balances of TNFα/sTNFRI and IL-6/IL-10 shifted towards inflammatory side.Furthermore, the changes of them reflect the varieties of cardiac function. 相似文献
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以亲缘关系极近的新高系梨10个品种及被视为‘新高’亲本的‘天之川’和‘今村秋’梨等为试验材料,对其基因组DNA进行了PCR-RFLP检测、DNA序列测定及生物信息学分析。结果表明：新高系梨10个品种即‘新高’、‘黄金’、‘水晶’、‘早生黄金’、‘早蜜新高’、‘天皇’、‘金秋’、‘晚大新高’、‘农家新高’和‘鲜黄’的S基因型分别为S3S9、S3S4、S3S9、S3S4、S3S9、S3S9、S3S9、S3S9、S3S9和S3S5;‘天之川’、‘今村秋’的S基因型分别为S1S9和S1S6,通过基因型判定‘今村秋’非‘新高’梨的父本。 相似文献
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XIE Tong-jie HONG Bing-zhe LI Sheng-fan WANG Li-ping PIAO Hai-nan GAO Li-jian LIU Xue-tian CHEN Yu-ting 《园艺学报》2008,24(9):1665-1669
AIM:The mechanism of angiopoietin-1 (Ang-1) in mediating increase in intracellular free magnesium (［Mg2+］i) in human umbilical vein endothelial cells (HUVECs) was investigated in this study. METHODS:The change of ［Mg2+］i in HUVECs was quantitatively detected in intracellular cation measurement system via loaded with the fluorescent magnesium indicator mag-fura-2. RESULTS:Ang-1 increased ［Mg2+］i, and there was not any significant difference among the groups of 0 mmol/L and 1 mmol/L of extracellular Mg2+. Similar results were obtained in groups done with Ca2+. Pretreatment with tyrosine kinase inhibitors (tyrphostin A23 and genistein), phosphatidylinositol 3-kinase (PI3K) inhibitors (wortmannin and LY294002) blocked the increase in ［Mg2+］i induced by Ang-1. However, mitogen-activated protein kinase inhibitors (SB202190 and PD98059) had no effect on the Ang-1-induced ［Mg2+］i increase. CONCLUSION:These results suggest that the increase in ［Mg2+］i by Ang-1 come from intracellular Mg2+ pools mediated by tyrosine kinase/PI3K -dependent signaling pathways. 相似文献
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AIM:To study the effect of environment stress on reproductive physiology in Chinese tree shrew．METHODS: 30 animals were divided at random into three groups.Group 1: 10 of 30 animals served as control;group 2 and 3 the 8 and 12 of 30 animals served as experiment and housed in small cage.The levels of the testosterone(T) and estradiol (E2) were determined by the method of radioimmunoassay and section preparation of testis was observed by light microscope after living in the reduced scope for 21days.RESULTS: The result showed that the levels of the testosterone in group 2 and 3 were decreased by 49.4% and 80.3%,respectively,compared to that in group1.The convoluted tubules of testis in group 2 and 3 were damaged and only a few spermatogonia was doserved.CONCLUSION: Social environment stress has significantly inhibitory effect on testosterone formation and destroy the testis structure of Chinese tree shrew. 相似文献