共查询到20条相似文献，搜索用时 31 毫秒
1.
AIM: To investigate whether celecoxib induces gastric cancer cell apoptosis in a COX-2 non-expression cell line. METHODS: The COX-2 protein was examined by western blotting. Fluorescence microscopy, DNA agarose gel electrophoresis and flow cytometry analysis were used to test apoptosis. RESULTS: COX-2 was expressed in AGS but not MGC-803 gastric cancer cell line; Selective COX-2 inhibitor celecoxib induced MGC-803 cell line apoptosis in a concentration and time-dependent manner. CONCLUSION: Celecoxib induces apoptosis in COX-2 non-expression gastric cancer cells. 相似文献
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AIM: To observe the influence of human mutant p27 gene (p27mt) on the growth and so as to investigate the function and mechanism of p27mt in gene therapy for colorectal cancer.METHODS: Colorectal cancer cell line SW480 was infected with recombinant replication defective adenovirus Ad-p27mt,and expression of p27mt protein was detected by Western blotting.The inhibitory effect of p27mt on SW480 and cell cycle were determined by flow cytometry,and DNA fragment was analyzed to identify the occurrence of apoptosis.RESULTS: After transfected with Ad-p27mt,p27 protein was highly expressed in SW480 cells.77.96% colorectal cancer cells were blocked in phase G0/G1,while in Ad-LacZ group and blank control group,27.57% and 25.29% cells were blocked in the same phase,respectively.Growth curve showed Ad-p27mt had an obviously inhibitory effect on the growth of SW480 cells.DNA fragment assay demonstrated that p27mt was able to induce the apoptosis of colorectal cancer cells.CONCLUSION: p27mt has an obvious blocking effect on colorectal cancer cell cycle,and most cells are blocked in phase G0/G1.This blockage is related with the growth inhibition and apoptosis induced by p27mt. 相似文献
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LUO Hai-bing WANG Li-wei MAO Jian-wen JIAO Cheng-gang FAN Ai-hui NIE Si-huai LI Pan CHEN Li-xin 《园艺学报》2007,23(2):226-230
AIM:To investigate the inhibitory effects of Cl- channel blocker, tamoxifen, on volume-activated Cl- currents of nasopharyngeal carcinoma cells (CNE-2Z cells) in G1 and S phases. METHODS:Highly synchronous cells in G1 phase and S phase were obtained by the serum starvation and the double-block techniques. The whole-cell patch clamp technique was used to observe the effects of tamoxifen on volume-activated Cl- currents and to analyze the anion permeability of volume-activated Cl- channels. RESULTS:47% hypotonic stimulation activated a Cl- current in the nasopharngeal carcinoma cells at the cell cycle stage G1 phase and S phase. Tamoxifen at concentration of 10 to 30 μmol/L completely inhibited the current. However, the time needed to completely inhibit the current was dose-dependent and was different between G1 phase and S phase. The time needed to completely inhibit the current was shorter in G1 cells than that in S phase cells. The anion permeability sequence of the volume-activated Cl- channel was I->Cl->gluconate in both G1 phase and S phase cells. The permeability of G1 phase cells to I- was higher than that in S phase cells, but to gluconate was lower than that in S phase cells. CONCLUSIONS:The density of the volume-activated Cl- current, the anion permeability of the channel and the sensitivity of the current to tamoxifen were different between the CNE-2Z cells in G1 phase and those in S phase. The results suggest that the expression of tamoxifen-sensitive, volume-activated chloride channels is differentiated at different stages of the cell cycle. 相似文献
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AIM: To study the mechanism of oleanolic acid induced apoptosis and its influence on cell cycle in HL-60 cells in vitro. METHODS: The HL-60 cells were treated with different concentrations of oleanolic acid and then cultured for 12 h, 24 h, 48 h and 72 h, respectively. MTT assay was used to evaluate the inhibitory effect of oleanolic acid on HL-60 cells in vitro. The argarose gel electrophoresis was used to detect the chromatin DNA fragmentation. FACS was used to analyze the cell cycle of HL-60 cells. Western blotting was used to detect the activation of caspase-3 which has been confirmed the last execution of apoptosis pathway. RESULTS: MTT assay showed that oleanolic acid dramatically inhibited the growth of HL-60 cells in vitro, more than 50% HL-60 cells were inhibited when the cells were treated with 80 μmol/L oleanolic acid for 48 h; the apparent DNA ladder was detected after exposure of HL-60 cells to oleanolic acid for 48 h. FACS analysis showed that cell cycle of HL-60 cells was arrested in G1 phase, the inhibition ratio of HL-60 cells achieved 63.24% and 67.90% after treated with oleanolic acid for 48 h and 72 h correspondingly. Western blotting detected the activation of caspase-3 after exposure of HL-60 cells to 80 μmol/L oleanolic acid for 48 h. CONCLUSION: These results suggest that oleanolic acid induces apoptosis and the cell cycle of HL-60 cells is arrested in G1 phase. 相似文献
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AIM: To investigate the effects of double thymidine deoxyribonucleoside (TdR) blocking on the cell cycle of human gastric cancer SGC-7901 cells.METHODS: SGC-7901 cells in the logarithm period were selected in the study and treated with TdR at concentration of 2 mmol/L, 4 mmol/L or 8 mmol/L for 15 h as the first blocking. After incubation in TdR-free medium for 10 h, the cells were treated with TdR at same concentrations again for another 15 h as the second blocking. The blocked cells were released by washing in fresh medium twice and incubation in TdR-free medium containing 10% fetal bovine serum for 12 h. The cells were collected and the cell cycle was detected by flow cytometry.RESULTS: By double TdR (2 mmol/L, 4 mmol/L and 8 mmol/L) blocking to synchronize the cell cycle, the cells in G0/G1 phase accounted for 77.3%, 77.5% and 77.0%, respectively. After further incubation for 12 h in TdR-free medium, the proportion of the cells in each phase of the cell cycles returned to normal range. CONCLUSION: The method of double TdR blocking is an ideal access in short term to acquire a large number of cells in G0/G1 phase. 相似文献
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AIM: To investigate the role of Mcl-1 in the G2/M arrest induced by diallyl disulfide (DADS) in leukemic HL-60 cells.METHODS: The inhibitory effect of DADS on human leukemic HL-60 cells was detected by CCK-8 method in vitro. Flow cytometry analysis was employed to observe the cycle arrest in HL-60 cells and the effect of DADS-induced G2/M arrest on HL-60 cells with Mcl-1 gene knockdown by RNAi silencing. The expression of Mcl-1, PCNA and CDK1 in HL-60 cells treated with DADS was determined by Western blotting. The binding of Mcl-1 with PCNA and CDK1 was detected by coimmuno-precipitation. RESULTS: HL-60 cells were treated with DADS at concentration of 15, 30, 60, 120 or 240 μmol/L for 48 h. The inhibition rates of HL-60 cell proliferation were 31.15%, 55.88%, 66.14%, 75.29% and 80.35%, respectively, and gradually enhanced with the increase in the concentration of DADS (P<0.05). Flow cytometry analysis revealed that the proliferation of HL-60 cells was blocked by DADS in the G2/M phase. Treatment with DADS for 24 h and 48 h at concentrations of 60 μmol/L and 120 μmol/L, the percentage of G2/M phase cells increased as compared to the untreated cells (P<0.05). DADS induced arrest of HL-60 cells in G2/M phase in a time- and dose- dependent manner (P<0.05). The results of Western blot analysis indicated that Mcl-1, PCNA and CDK1 in HL-60 cells were significantly reduced after treated with DADS (P<0.05). HL-60 cell cycle progression delayed by silencing Mcl-1 gene with siRNA technique, suggesting that silence of Mcl-1 gene led to G2/M arrest. Compared to the cells treated with DADS only, the percentage of G2/M cells raised in the cells with Mcl-1 gene silencing and treated with DADS (P<0.05), indicating that Mcl-1 gene silencing enhanced the effect of DADS-induced G2/M arrest in HL-60 cells. The binding of Mcl-1 with PCNA and CDK1 was detected by coimmuno-precipitation and the formation of heterodimers was observed, which was decreased after treated with DADS for 4 h.CONCLUSION: DADS inhibits the proliferation of HL-60 cells and induces its G2/M phase arrest. The decreased expression of PCNA is related to inhibiting the proliferation of leukemic cells. Knockdown of Mcl-1 gene enhances the effect of DADS-induced G2/M arrest. 相似文献
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AIM:To investigate the anti-tumor effect of Weimaining (WMN) on a murine Lewis lung carcinoma (3LL) and the influence on the cell cycle. METHODS:The inhibitory rate of WMN in 3LL growth was detected by replicating the model of 3LL. The effect of the drug on 3LL cell cycle and the influence of the drug on the expression of cyclin D1 protein were investigated by flow cytometry and immunohistochemical staining. RESULTS:The results showed that the inhibitory rate of drug in 3LL is 19.14%, 33.59%, 40.63% and 51.56% respectively at dosage ranging from 100,150, 200 and 250 mg·kg-1·d-1. The drug inhibits tumor growth in a dose-dependent manner. The drug arrests 3LL cells in G0-G1 phase and decreases the expression of cyclin D1 protein. CONCLUSION:WMN inhibits the growth of 3LL cells in vivo by decreasing the expression of cyclin D1, blocking the cells in G0-G1 phase and preventing the cells transition from G1 to S phase while DNA is replicated. 相似文献
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ZHOU Lei LI Yang SU Jing KANG Jin-song MU Gui-fang WANG Zhi XU Li ZHAO Xue-jian SUN Lian-kun 《园艺学报》2007,23(7):1339-1342
AIM: To study the effect of vitamin K3 (VK3) on the induction of apoptosis in androgen-independent prostate cancer cell PC-3M in vitro.METHODS: Cell viability was estimated by MTT assay. AO/EB staining was performed to detect apoptotic cells. Apoptosis and the changes of cell cycle were detected by flow cytometry. NAC was used to observe the effect of growth inhibition by VK3. RT-PCR was used to confirm the changes in gene expression. Levels of intracellular peroxides were estimated by using an oxidation-sensitive fluorescent probe DCFH-DA. RESULTS: PC-3M cells growth was significantly inhibited by VK3 (≥60 μmol/L, P<0.05). The inhibitory effect was time and dosage dependent. The result of AO/EB staining showed that apoptosis of PC-3M cells were induced by VK3. A typical subdiploid peak before G0/G1 phase was observed after treated for 12 h with VK3 (60 μmol/L) by flow cytometry. The effect of growth inhibition treated with VK3 was antagonized by antioxygen NAC (5, 10, 20, 40, 80 μmol/L). An increase in the level of DCF fluorescence after PC-3M cells were treated for 1-2 h with VK3 was observed. Antioxidase GSH-Px and CAT were run-down after treated with VK3. CONCLUSION: The results indicate that apoptosis in PC-3M cells is induced through oxidative stress by VK3. 相似文献
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AIM: To investigate the effect of norcantharidin（NCTD）on proliferation and invasion of human breast cancer cell line SKBR3 in vitro and its anticancer mechanisms.METHODS: MTT assay was used to determine SKBR3 cell proliferation. Light and FACScan were used to detect apoptosis and cell cycle. The invasiveness of SKBR3 was evaluated by the adhesion test，Matrigel experiment and the crossing-river test.RESULTS: NCTD had inhibitive effects on growth of SKBR3 cells in a dose and time-dependent manner, with the IC50 value of 12.5 mg/L at 24 h．The cells treated with 10 mg/L NCTD for 24 h and 48 h showed typical apoptotic morphology and hypodiploid peak before G1 phase. The cell cycle was arrested at G2/M phase. The apoptosis percentage was up to 3.44% and 6.17%, and the G2/M percentage was up to 35.82% and 38.70%. NCTD also could inhibit obviously the adhesion, movement and invasive capability simulating human basement membrane of SKBR3. Its effect was also in a dose-dependent manner. In the NCTD-treated group, crossing-river time was prolonged significantly and passing-membrane cells markedly decreased. CONCLUSION: NCTD in vitro inhibits not only the proliferation and growth of human breast cancer cells but also invasion and metastasis of the cells at relatively low concentration. NCTD shows prominent anti-tumor effects. 相似文献
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KUANG Yan-ping CHEN Ken HE Bo-hua WANG Lin-jing ZHU Ai-zhen LIU Cheng-cheng LIU Ge-xiu 《园艺学报》2012,28(7):1247-1252
AIM: To investigate the effect of photoactivated curcumin on apoptosis of human gastric cancer MGC-803 cells. METHODS: The effect of photoactivated curcumin on the growth inhibitory rate of gastric cancer MGC-803 cells was detected by MTT assay. The changes of nuclear morphology were observed under optical microscope with Hoechst 33258 fluorescent staining. The apoptotic rate, mitochondrial membrane potential, intracellular reactive oxygen species and Ca2+ level was determined by flow cytometry. The activity of caspase-3, caspase-8 and caspase-9 was detected by colorimetry. The protein levels of cytochrome C, Bcl-2, Bax and heat-shock protein 70 (HSP70) were analyzed by Western blotting. RESULTS: The growth inhibitory rate of MGC-803 cells treated with curcumin at concentration of 5.0 μmol/L was (29.74±2.30)%.Some apoptotic cells were observed under optical microscope, and the apoptotic rate was (12.54±1.75)%. The growth inhibitory rate of MGC-803 cells treated with photoactivated curcumin was (44.93±3.61)%.Significant morphological changes in the nucleus, such as chromatin condensation and apoptotic body formation, were observed under light microscope, and the apoptotic rate was (26.58±2.67)%. The cell cycle was arrested at G0/G1 phase. Compared with curcumin group, significant reduction in mitochondrial membrane potential,significant increase in cytochrome C, intracellular reactive oxygen species, Ca2+ level and the activity of caspase-3, caspase-8 and caspase-9 were observed in photoactivated curcumin group (P<0.01). Photoactivated curcumin also significantly inhibited the protein expression of Bcl-2 and HSP70 in the cells. CONCLUSION: Photoactivated curcumin enhances the apoptosis of gastric cancer MGC-803 cells by inhibiting Bcl-2 expression and promoting the mitochondrial pathway. 相似文献
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AIM: To observe the effects of tissue extracts of injured liver on BEL-7402 cells,and explore a novel strategy of tumor therapy by differentiation induction. METHODS: Differentiation induction of human hepatocellular carcinoma cell line BEL-7402 was carried out with liver tissue extracts from an animal model of liver injury. The changes of cell biological characteristics, such as morphological features of the cells, MTT growth curves and cell cycle distribution, were dynamically observed. RESULTS: After exposed to the tissue extracts of injured liver, the number of mitotic cells was decreased, and the speed of growth and the proliferation of carcinoma cells were slowed down dramatically. The percentage of the cells in G0/G1 phase was increased, while the cells in S phase was decreased. The level of proliferation index (PI) also declined. CONCLUSION: The tissue extracts of injured liver affect the differentiation status of human hepatocellular carcinoma cell line BEL-7402, promote the cell differentiation and reduce the tumor characteristics. The tissue extracts of injured liver possess an important potential as a tumor differentiation inducer. 相似文献
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MA Hui-xiang XU Jin-tang JIANG Zhen-you ZHANG Sui-mei ZHAO Song-bin CHEN Jian-su 《园艺学报》2008,24(5):915-919
AIM: To investigate the effects of rhTGF-β1 and TGF-β1 gene transfection on the proliferation of cultured rabbit corneal endothelial cells in vitro. METHODS: Cell growth induced by various concentrations of rhTGF-β1 was determined by MTT proliferation assay. Under the induction of liposomes, recombinant pSecTag2-TGF-β1MP vectors were transferred into the corneal endothelial cells. Morphological changes of transfected cells were observed by HE staining. The expression levels of TGF-β1 were assessed by ELISA. Cell cycle analysis was assessed by flow cytometry. DNA fragment analysis was used to confirm the presence of apoptosis. RESULTS: rhTGF-β1 in concentrations of 5-20 μg/L showed a significant suppressive effect on the proliferation of corneal endothelial cells, 0.5-1 μg/L had no effect, 0.05-0.1 μg/L facilitated cell growth, as compared with negative controls. The morphous of transfected corneal cells had no significant abnormality compared with normal cells. According to the result of ELISA, the concentration of TGF-β1 in the supernatant was calculated to be (98±3) ng/L. Flow cytometry assay showed that S and G2/M phase of transfected cells decreased significantly compared with that of control group, but the cell cycle recovered normally after adding 10 μg/L EGF into the culture medium. Agarose electrophoresis didn′t show marked ladders in transfected group. CONCLUSION: Effects of rhTGF-β1 on the proliferation of corneal endothelial cells are different with various concentrations. TGF-β1 gene transfection shows suppressive effect on the proliferation of cultured corneal endothelial cells, but does not induce cell apoptosis. EGF is the antagonist of this suppressive effect. 相似文献
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AIM:To investigate the neuroprotective effect of tubuloside B, one of the phenylethanoids isolated from the stems of Cistanche salsa, on H2O2-induced injury in PC12 cells.METHODS:PC12 cells were exposed to various doses of tubuloside B for 12 h, then treated with H2O2 at concentration of 100 μmol/L for 24 h. The cell viability was observed with MTT assay. Reactive oxygen species and the mitochondrial membrane potential were measured with laser scanning confocal microscopy (LSCM). The DNA content and percentage of apoptosis were assayed by DNA agarose gel electrophoresis and flow cytometry. The activation of caspase-3 was detected with the caspase-3 activity assay kit. RESULTS:Following treatment with H2O2 for 24 h, H2O2 induced a significant decrease in cell viability; DNA ladder was observed and apoptosis percentage was as high as 48.0%. Accumulation of intracellular ROS, increase in caspase-3 activity and the decrease in mitochondrial membrane potential as indicated with the decrease of red/green ratios (from 5.97 to 0.41) were detected. However, pretreatment with tubuloside B (1, 10 or 100 mg·L-1) for 12 h exhibited cytoprotective effects in a dose-dependent manner. Tubuloside B obviously enhanced the cell viability, reduced formation of the DNA ladder, and significantly reduced the number of cells labeled with Annexin-V. The percentage of apoptosis/necrosis neurons was significantly decreased to 30.9%, 18.3% and 6.2%, respectively. LSCM showed that the tubuloside B attenuated the accumulation of ROS and the H2O2-induced collapse of mitochondrial membrane potential in PC12 cells. The significant decrease in caspase-3 activity was detected, compared to the H2O2-treated cells at the same time point. CONCLUSION:Tubuloside B has the neuroprotective capacity to antagonize H2O2-induced apoptosis and injury in PC12 cells, indicating it may be useful for treating some neurodegenerative diseases. 相似文献
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AIM: To evaluate the effects of NS-398, a cyclooxygenase-2(COX-2) inhibitor, on the proliferation and apoptosis in HepG2 cells. METHODS: The effects of NS-398 on the proliferation of HepG2 cells was evaluated by MTT. DNA fragmentation gel analysis was used to analyze the apoptotic cells; DNA ploidy and apoptotic cell percentage were examined by flow cytometry. Furthermore, the expression of COX-2 and Bcl-2 mRNA was identified by competitive RT-PCR. RESULTS: NS-398 inhibited cell proliferation and induced apoptosis in HepG2 in a concentration-dependent manner. DNA ploidy analysis showed that S phase cells were significantly decreased with NS-398 concentration increasing. The quiescent G0/G1 phase was accumulated with decreasing of Bcl-2 mRNA. Whereas NS-398 had no effect on the expression of COX-2 mRNA, no correlations were found between COX-2 mRNA and the HepG2 cell proliferation and apoptosis induced by NS-398 (r=0.056 and r=0.119, respectively). CONCLUSION: NS-398 significantly inhibits the proliferation and induces apoptosis in HepG2. Mechanisms may be involved in accumulation of quiescent G0/G1 phase and decrease in Bcl-2 mRNA expression, but independent to COX-2 mRNA expression. 相似文献
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AIM: To investigate the synergistic effect of decitabine (DCA) and valproic acid (VPA) on apoptosis and cell cycle arrest at G0/G1 phase in gastric cancer MGC-803 cells. METHODS: Gastric cancer MGC-803 cells were used in the study and divided into the following groups according to the treatment with different drugs for 72 h: DCA 1.5 μmol/L,DCA 3.0 μmol/L, VPA 1.5 mmol/L, DCA 1.5 μmol/L+VPA 1.5 mmol/L and DCA 3.0 μmol/L+VPA 1.5 mmol/L. The early and late apoptotic rates were detected by annexin V and PI staining. The cell cycle was also determined by flow cytometry. The relative nm23-H1 mRNA expression level was measured by real-time quantitative PCR. RESULTS: The apoptotic rates in VPA 1.5 mmol/L+DCA 1.5 μmol/L group (early: 33.58%±3.88%; late: 31.52%±4.20%) and VPA 1.5 mmol/L+DCA 3.0 μmol/L group (early: 42.61%±4.23%; late: 38.01%±3.86%), the percentages of the cells in G0/G1 phase in VPA 1.5 mmol/L+DCA 1.5 μmol/L group (61.55%±2.38%) and VPA 1.5 mmol/L+DCA 3.0 μmol/L group (66.75%±2.48%), and the relative nm23-H1 mRNA expression levels in VPA 1.5 mmol/L +DCA 1.5 μmol/L group (1.84±0.46) and VPA 1.5 mmol/L+DCA 3.0 μmol/L group (3.02±0.36) were all significantly higher than those in the corresponding concentrations of single drug treatment groups (P<0.01). CONCLUSION: Synergistic effect of VPA and DCA on apoptosis and cell cycle arrest in gastric cancer MGC-803 cells is possibly via inactivation of nm23-H1 gene expression. 相似文献
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AIM: To investigate the effects of leptin (LEP) on the alveolar type Ⅱ cells(AECⅡ) apoptosis induced by Na2S2O4 and explore the molecular mechanisms. METHODS: Primary AECⅡ culture was prepared according to a specific immunosorption procedure with slight modification and the cells were identified by transmission electron microscope and immunocytochemistry. AECⅡ damage was induced by 5 mmol/L Na2S2O4. LEP group cells were treated with LEP at concentrations from 100 μg/L to 1 600 μg/L. The cell survival rate was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Cell cycle and apoptosis were analyzed by flow cytometry and the level of caspase-3 was measured by Western blotting. RESULTS: Highly purified AECⅡ, obtained by the method of modified immunosorption, were identified with the positive expression of SP-A and intracellular lamellarbodies were found under electron micrography. The cells, exposed to 5 mmol/L Na2S2O4, showed characteristic changes of apoptosis and activation of caspase 3. These damages were relieved by the treatment of LEP (100-1 600 μg/L), with survival increasing, apoptosis peak decreasing, cell morphology restoring and caspase 3 activation inhibiting.CONCLUSION: Leptin prevents AECⅡ from apoptosis induced by Na2S2O4 or hypoxia. The potential mechanism of its action may be related to promoting cell cycle from G1 phase to S phase and inhibiting the activating of caspase 3. 相似文献
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CHEN Chuang-qi FANG Le-kun LIU Jia-wei ZHANG Jian-wei YANG Guang-xin YANG Wan 《园艺学报》2010,26(10):1908-1912
AIM: To investigate the effects of marine fungal metabolites 1386A from the South China Sea(1386A) on gastric cancer cell line MCG-803.METHODS: The effects of 1386A on cell viability and cell growth were detected by MTT method and colony-forming assay, respectively. The changes of cell cycle, cell apoptosis and mitochondrial membrane potential were determined by flow cytometry.RESULTS: The IC50 of cell viability on MCG-803 cells after treated with 1386A for 24 h, 48 h and 72 h was 29.70 μmol/L, 14.07 μmol/L or 13.47 μmol/L, respectively. The IC50 of the cell growth on MCG-803 cells after treated with 1386A for 48 h was 3.27 μmol/L. After treated with 1386A for 48 h, the MCG-803 cells in S stage was increased from 25.6% to 56.8%. After treated with 1386A for 24 h, the early apoptosis of MCG-803 cells was increased from 3.34% to 8.39%, and the mitochondrial membrane potential of MCG-803 cells was decreased by 56.26%.CONCLUSION: 1386A has an inhibitory effect on the cell growth in MCG-803 cells through the mitochondrial pathway. 相似文献
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AIM: To investigate whether endogenous endothelium-derived hyperpolarizing factors (EHDFs) produced by CYP epoxygenases BM3·F87V,2C11OR or CYP2J2 transfection was able to protect endothelial cells against apoptosis induced by tumor necrosis factor alpha.METHODS: Three or four passages of cultured bovine aortic endothelial cells (BAECs) were transfected with epoxygenases or the empty vector (pCB6).Cell viability was detected by MTT assay.Apoptosis of transfected endothelial cells was evaluated by DNA ladder assay,flow cytometry and morphological observations under fluorescence microscopy.RESULTS: Overexpression of CYP epoxygenases BM3·F87V,2C11OR,CYP2J2 increased cell viability respectively observed by MTT assay.The percentage of cells undergoing apoptosis was decreased in 2C11OR-, BM3F87V- or 2J2-transfected cells compared to the vector as evaluated by DNA fragment assay,flow cytometry analysis and morphological observations under fluorescence microscopy.CONCLUSION: Overexpression of CYP epoxygenases BM3·F87V,2C11OR or 2J2 increases cell viability and protects endothelial cells against TNF-α-induced apoptosis.These findings suggest new targets to investigate the endothelium-associated disorders and provide novel therapeutic strategies to treat them by modulating cytochrome P450 epoxygenases. 相似文献