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1.
Summary The suitability of large-fruited apple cultivars as alternative donors of powdery mildew resistance to the crabapples carrying major gene resistances was assessed. Five accessions with varying levels of mildew resistance (‘Dülmener Rosen', ‘Liberty', ‘Sciros', ‘Prima' and ‘Red Delicious') and one susceptible cultivar (‘Cox's Orange Pippin') were mated in a partial diallel design, and sub-sets of each seedling family were evaluated for three years at three New Zealand sites (Havelock North, Riwaka and Clyde) with different climatic conditions. There was a high correlation among the powdery mildew susceptibility scores of the individual seedlings over the three years, but disease levels varied among sites, with those at Clyde being the highest. All accessions with some resistance were found to confer additive resistance to their progenies to varying degrees depending on the climatic conditions of the sites, but findings suggest that progeny selection at Clyde will be sufficient for breeding purposes. The performance of the cultivar itself was not necessarily a good predictor of the level of resistance conferred to its progenies. The mostly negative genetic correlations between powdery mildew scores and fruit quality attributes suggest that selecting for powdery mildew resistance and desirable fruit quality traits may not be difficult. However, a larger number of families may be needed for more precise estimates of the genetic correlations to strengthen confidence in this conclusion.  相似文献   

2.
Summary The inheritance of field resistance to downy mildew in lettuce derived from cv. Grand Rapids was studied in F2, F3 and F4 progeny from a cross with the more susceptible cv. Lobjoits Green Cos. The results for 32 randomly produced F3 families and the F2 population corresponded closely although the absolute level of resistance varied from season to season according to disease pressure and environmental conditions. The field resistance character showed a quantitative pattern of inheritance but the rapid response to selection at F3 implied that only a few genes may be involved. F4 families raised from families selected from the two extremes of the F3 distribution fell into distinct categories with resistance levels corresponding to that of their F3 parents. An association between resistance and morphological features of the resistant parent was present at F2 but not in subsequent generations when individuals and families which were as resistant as cv. Grand Rapids and yet bore little morphological resemblance to this cultivar were readily recovered.  相似文献   

3.
I. Batlle  F. H. Alston 《Euphytica》1994,77(1-2):11-14
Summary The isoenzymic geneGOT-1 was used as an indicator of ploidy levels and hybridity in the progeny of a 4x × 2x (A878/5,GOT-1 abdn × ‘Gloster 69’,GOT-lcc) cross, derived from the apomictic species (Malus hupehensis), which segregated for mildew resistance. The distinction between resistant and susceptible plants was sharp. After 3 months in a greenhouse, resistant plants showed no signs of sporulation. Of the hybrid seedlings (diploid and triploid) 78% were mildew resistant. This analysis was facilitated through the use of theGOT-lc allele, as a marker of hybridity. It appears that the prospects of transferring high mildew resistance fromM. hupehensis to the cultivated apple are promising; of the 65 hybrid resistant seedlings 18 were diploid. A similar approach in earlier generations might have facilitated the earlier transfer of this resistance to the cultivated apple.  相似文献   

4.
Summary F2-plant progenies, derived from seven crosses between susceptible erectoides breeding lines and conventional, nutans breeding lines with partial resistance to leaf rust incited by Puccinia hordei Otth., derived from Vada x Cebada Capa, were examined in a field and a glasshouse experiment. In the field experiment, the nutans plant progenies generally were more resistant to leaf rust and powdery mildew than the erectoides progenies. In the glasshouse experiment, the components of partial resistance to leaf rust of six erectoides and six nutans progenies derived from one cross were studied. The nutans progenies generally had lower infection frequencies than erectoides progenies with a similar infection level in the field experiment. The variation for leaf rust and powdery mildew infection in the field was similar for the nutans and erectoides progenies. This suggests that erectoides lines with a fairly high level of partial resistance to leaf rust and powdery mildew can be selected from these populations.  相似文献   

5.
Summary Several thousand apple and pear seedlings of many progenies were studied in connection with their juvenile period (J.P.). The initially significant inverse relation between the vigour (stem diameter) and the J.P. of the seedlings became insignificant as the trees grew older, due to a retardation of growth occurring when the seedlings become generative. This relation can be used effectively in pre-selecting for vigour in the nursery. Cumulative yields were higher when the J.P. was shorter, but there was no evident link between the J.P. and annual yield in full bearing, that is to say, precocity and preductivity are not directly connected. Better growing conditions and pre-selection have shortened the mean progeny J.P. of either crop by three years since the 1950's. Generally, the pear seedlings appear to grow faster, to become generative slower and to yield lower than comparable apple seedlings. The mean J.P. of apple and pear progenies averaged 4.2 and 6.0 years respectively. The difference between apple and pear may be attributable to a greater selection pressure on both precocity and productivity with apple than with pear.This possibly also led to a smaller tree in the productive phase on the assumption that tree size at first flowering-small at a short, large at a long J.P.-expresses reproductive efficiency and as such is reflected in the size of the adult tree.Also presented at the Juvenility Symposium held at West-Berlin, November 1976.  相似文献   

6.
Cucurbita pepo (pumpkin, squash,gourd) is an economically important species that is susceptible to the cucurbit powdery mildew fungus, Podosphaera xanthii(syn. Sphaerotheca fuliginea). ‘True French’, an open-pollinated cultivar of the Zucchini Group of C. pepo, was crossed with an unnamed powdery-mildew resistant straight neck-type accession, the resistance of which was apparently derived from an interspecific cross with a resistant wild species of Cucurbita,and resistant plants were selected in the F2 generation. This was followed by six cycles of backcross-pedigree selection for resistance, and resulted in the development of an accession true-breeding for resistance to powdery mildew and nearly isogenic to ‘True French’. The resistant and susceptible near-isogenics were crossed and seeds of the filial and backcross generations were produced. Plants of the parental accessions and their progenies were grown together in a controlled-environment chamber, exposed to the pathogenic fungus, and scored as resistant, partially resistant, or susceptible 27–33 days after sowing. The results indicated that resistance is conferred by a single incompletely dominant gene, designated Pm-0. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

7.
Summary Previously identified segregant lines of oats with levels of adult plant resistance to powdery mildew (Erysiphe graminis f.sp. avenae) better than the resistant parent maintained this high level of resistance in field nurseries over two growing seasons. This enhancement was not expressed on inoculated detached leaves under laboratory conditions where no differences between the more resistant parent cv. Maldwyn and the most resistant segregants was detected. Reduced resistance was not detected in tests where leaf segments of the transgressive lines were inoculated with trained isolates. Problems associated with selecting for adult plant resistance under both field and laboratory conditions are discussed.  相似文献   

8.
Summary Partial resistance to powdery mildew in spring barley was evaluated in three plot types: large isolation plots, in 1.4 m2 plots in chessboard design with guard plots of spring wheat and in single rows. Percentage leaf area covered by powdery mildew was scored four to six times during the season and partial resistance was characterized by the area under the disease progress curve. Varietal differences were revealed in al three plot designs, differences between the most resistant and susceptible genotypes being of a factor five. Differences between varieties decreased with decreasing plot size. The relationship between single scores of amount of powdery mildew on the upper four leaves and the area under the disease progress curve was high in all plot designs during the first two to three weeks after heading, allowing selection for the trait by one or two scorings. Differential ranking of varieties between different plot designs was observed, and is assumed to be due to increasing plot interference with reduced plot size and reduced distance between plots. A reliable selection for partial resistance could be made in large isolation plots and in 1.4 m2 plots, but hardly in single rows.  相似文献   

9.
Summary Six spring barley genotypes with growth stage dependent expression of partial resistance to powdery mildew were crossed following a half diallel scheme. Data for percentage infected leaf area at different growth stages from the F1 and F2 generation and the F2 offspring were analyzed. The absence of a contrast between parents and offspring indicated that dominance effects were not important at the young growth stages. Diallel analysis showed that general combining ability effects (GCA) were important at all growth stages. Although significant, specific combining ability effects were of minor importance. The cross between the most resistant parents with the largest, negative GCA resulted in the most resistant progeny.  相似文献   

10.
Summary Eight scab-resistant cultivars and selections along with eight commercial apple cultivars were evaluated for powdery mildew resistance in greenhouse and nursery tests. Dayton, Liberty, Delicious and Tolman Sweet were rated moderately resistant to infection in both greenhouse and nursery tests. Segregation of seedlings among 14 progenies for mildew reaction indicated that mildew resistance is polygenically controlled in this material with additive gene effects. Recovery of mildew resistant seedlings from crosses involving a scab-resistant parent(s) suggested that this material can be useful in developing scab- and mildew-resistant apple cultivars. Histological investigations were conducted to describe mildew symptoms of infected leaves.  相似文献   

11.
Selection for brown stripe downy mildew resistance in maize   总被引:3,自引:0,他引:3  
Summary The maize (Zea mays L.) cultivar Makki Safed 1 (MS1) with susceptibility to brown stripe downy mildew (BSDM) caused by Sclerophthora rayssiae var. zeae Payak & Renfro, was subjected to two cycles of mass selection and one cycle of full-sib family selection. Selection was carried out primarily for BSDM resistance.The mass selection was practised under artificial epiphytotic conditions in a disease nursery. Full-sib progenies and performance trials on MS1 and its improved versions were grown in diseased and disease free environments.Mass selection resulted in a significant improvement for resistance to BSDM. A cycle of full-sib selection resulted in an additional improvement for resistance to the disease. The disease rating of the improved version was 1.5 against 4.5 for the original population (scale: 1, highly resistant to 5, highly susceptible). The yield of the improved populations of MS1 was significantly greater than that of MS1 in the disease nursery. In disease free experiments, the improved populations showed almost no yield advantage over MS1. There were also no significant differences between the original population and the improved population after three selection cycles for ear length, ear girth, number of kernel rows per ear, number of kernels per row, 1000-kernel weight, plant height, ear height and days to silk.  相似文献   

12.
Summary Dihaploids were produced from tetraploids resistant to potato cyst nematode (Globodera pallida (Stone)). High levels of resistance were found in the dihaploids and three were used to produce tetraploid progenies by crossing them with susceptible tetraploid cultivars. One dihaploid, PDH505, produced more highly resistant offspring than the other two, PDHs 417 and 418. The latter gave progenies whose levels of resistance were similar to those obtained from susceptible dihaploids crossed with resistant tetraploids.The differences between the progenies of the resistant dihaploids were probably due to different modes of unreduced gamete formation (PDH505 producing gametes by first division restitution (FDR) and PDHs 417 and 418 by second division restitution (SDR)) although cytological studies would be necessary to confirm this. The methods by which dihaploids could be utilised in a tetraploid potato breeding programme are discussed in relation to the mode of unreduced gamete formation.  相似文献   

13.
Summary A collection of 133 genotypes consisting mainly of old European barleys and land varieties was assessed for level of partial resistance to barley mildew at the adult plant stage. Identification of any known resistance factors conferring hypersensitive reactions to the mildew races present at various frequencies in N.W. Europe was ensured through seedling tests with an appropriate AO mildew isolate.Thirty-nine varieties with relatively high levels of adult (partial) resistance and lacking known race-specific resistance genes were tested over a period of three years in field disease nurseries. At least thirteen varieties showed significantly less percentage leaf area infected than the control variety Proctor. These constitute a useful reserve of non-hypersensitive resistance, probably under the control of genes with mainly additive effects. In combination, these genes give enhanced levels of resistance, which could be of a more stable nature.  相似文献   

14.
Summary The first backcross and F2 progenies from triploid F1 and tetraploid F1 hybrids between B. napus and 2x and 4x B. oleracea ssp. capitata (cabbage) were studied for their general morphology, resistance to race 2 of the clubroot pathogen, chromosome number and meiotic chromosome behavior. No linkage was apparent between resistance and the major morphological characters. Unreduced gametes played a large part in the successful formation of seed of the B1 and F2 progeny. B1 plants with low chromosome numbers were selected for use in recurrent backcrosses. The potential use of anther culture to extract gametic progenies from resistant B1 and F2 plants with higher chromosome numbers was suggested. The presence of homoeologous pairing observed in all the plants is considered advantageous for selecting suitable progeny in later generations.  相似文献   

15.
Summary Effects of resistance genes and heat tolerance genes on expression of resistance to bacterial wilt caused by Pseudomonas solanacearum were investigated in 30 F1 progenies from parents with different levels of bacterial wilt resistance and heat tolerance. A race 1 and a race 3 isolate of the bacterium were used for inoculation under screenhouse conditions at two locations. Results obtained indicated that with reduction in levels of parental resistance, resistance in the F1 progenies was also reduced. Under hot conditions, a reduced heat tolerance in the genetic background also resulted in lower levels of resistance expression. The effect of heat tolerance tended to diminish at lower temperatures leaving the effect of resistance genes more consistent. There existed a strong interaction between resistance genes and genes for heat tolerance. The nature of resistance to bacterial wilt in potato and implications for breeding for resistance are discussed.Effects of reciprocal crosses on expression of resistance to a race 1 isolate under hot screenhouse conditions, were studied in 5 sets of reciprocal F1 progenies involving different resistant and susceptible parents. The reciprocal differences observed were not significant suggesting absence of cytoplasmic effects on expression of resistance.  相似文献   

16.
Summary The oat line Pc54 was found to be resistant to powdery mildew under both field and glasshouse conditions. The ratio of resistant to susceptible F2 and F2 progeny of a cross between a selection from the Pc54 line (Cc7422) and a susceptible cultivar (Selma) showed that, in addition to carrying the crown rust resistance gene Pc54 and the pg15 gene for stem rust resistance, the mildew resistance of the Pc54 line was conditioned by a single incompletely dominant gene along with additional factors which modified the expression of resistance. Previous results, that there was no linkage between genes Pc54 and Pg15, were confirmed. In addition, there was no evidence of linkage between the mildew resistance gene and gene Pc54. Evaluation of selections from within the Pc54 line showed that the expression of both stem rust and mildew resistance was modified by, or linked to, plant height. The effectiveness of genes Pc54 and Pg15, as measured by virulence frequencies, in central and eastern Europe is described.  相似文献   

17.
Resistance to four foliar diseases of sugar beet (Beta vulgaris ssp. vulgaris), virus yellows caused by Beet mild yellowing virus (BMYV) and Beet yellows virus (BYV), powdery mildew (Erysiphe betae) and Cercospora leaf spot (Cercospora beticola), was assessed in up to 600 accessions of closely related wild and cultivated Beta species. Most accessions were from the Section Beta, a taxon containing types most closely related to, and sexually compatible with, sugar beet and therefore most valuable for use in crop improvement. Between 1–12% of accessions were highly resistant (resistance scores of 2 on an international standardised resistance scale of 1–9) to these diseases. These levels, however, underestimate the potential number of resistant sources available from this section as some accessions with intermediate mean resistance scores contained a significant proportion of highly resistant plants within segregating populations. Variation in resistance to all diseases except BYV was observed within the Section Beta. Much higher levels of resistance were observed, and more frequently, in more distantly related sections of the genus Beta. Accessions of the Section Corollinae were highly resistant to both viruses (>62% of accessions tested), but less so to Cercospora (15%) and they were very susceptible to powdery mildew. Section Procumbentes accessions were highly resistant to BMYV and Cercospora (100%) but less so to powdery mildew (50%) and BYV (20%). However, sexual incompatibility between these sections and sugar beet make utilisation of these sources impractical using conventional breeding methods.  相似文献   

18.
Summary Verticillium wilt is a serious disease of potato and is caused by the soil-borne fungi Verticillium dahliae and V. albo-atrum. No major cultivar is resistant to this disease. Two diploid interspecific potato clones, C287 and C545, were previously identified with consistently high levels of Verticillium wilt resistance and are thought to have the same genotype for the loci that confer resistance to V. dahliae stem colonization. The purpose of this study was to determine whether resistance to V. dahliae stem colonization could be transferred to the tetraploid level in potato via unilateral sexual polyploidization (USP). Progenies in eight families obtained by crossing C287 and C545 to two tetraploid breeding clones, S438 and S440, and the cultivar ‘Atlantic’ were planted in a V. dahliae-infested field and a field without a significant V. dahliae infestation. Resistance was evaluated relative to C545 and C287. There were differences among families for stem colonization and yield. No differences between the mean stem colonization of C545 and C287 progeny were detected. Family differences due to the tetraploid parents indicate that they contributed resistance to the progeny. Stem colonization data from this experiment were consistent with the proposed complementary two-gene model for Verticillium wilt resistance in the diploid parents. Unilateral sexual polyploidization is an effective method for transferring V. dahliae stem colonization resistance to the tetraploid level.  相似文献   

19.
Summary In studies of the inheritance of resistance, pea seedlings of seven lines in which stems and leaves were both resistant to Mycosphaerella pinodes were crossed with a line in which they were both susceptible. With seven of the crosses resistance was dominant to susceptibility. When F2 progenies of five crosses were inoculated on either stems or leaves independently, phenotypes segregated in a ratio of 3 resistant: 1 susceptible indicating that a single dominant gene controlled resistance. F2 progenies of one other cross gave ratios with a better fit to 9 resistant: 7 susceptible indicating that two co-dominant genes controlled resistance. The F2 progeny of another cross segregated in complex ratios indicating multigene resistance.When resistant lines JI 97 and JI 1089 were crossed with a susceptible line and leaves and stems of each F2 plant were inoculated, resistance phenotypes segregated independently demonstrating that leaf and stem resistance were controlled by different genes. In two experiments where the F2 progeny of the cross JI 97×JI 1089 were tested for stem and leaf resistance separately, both characters segregated in a ratio of 15 resistant:1 susceptible indicating that these two resistant lines contain two non-allelic genes for stem resistance (designated Rmp1 and Rmp2) and two for leaf resistance (designated Rmp3 and Rmp4). Evidence that the gene for leaf resistance in JI 1089 is located in linkage group 4 of Pisum sativum is presented.  相似文献   

20.
L. Crespel  S. Gudin 《Euphytica》2003,133(1):65-69
The objective of this study was to determine the mode of inheritance of field resistance to downy mildew (Peronospora parasitica (Pers. ex Fr.) Fr.) in broccoli (Brassica oleracea var.italica) at the adult plant stage. The F1, F2 and F3 progeny of resistant and susceptible plants of broccoli were tested in the field under natural infection, in central Portugal, from August to December in two successive years. The plants were evaluated for resistance to downy mildew at maturity using a five-class scale of increasing susceptibility to the disease, which took into account the number of infected leaves and the size of the sporulating lesions. The F1 was completely resistant, the F2 segregated a clear 3 resistant: 1susceptible and the F3 confirmed the F2 segregation, which suggests a dominant character controlled by a single locus. This resistance has good potencial for direct use in commercial broccoli breeding or for transfer to other Brassica vegetables. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

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