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1.
Studies were performed to investigate factors affecting symptom expression of potato virus Y infection in three potato cultivars, Russet Norkotah, Shepody, and Red LaSoda. Quantitative enzyme-linked immunosorbent assay (ELISA) results revealed few differences in the relative virus titer among cultivars tested. Potato virus Y (PVY) titers developed as rapidly in Russet Norkotah as in Shepody and Red LaSoda. Additional studies were performed to determine the effect of light intensity and infections of PVY and potato virus X (PVX), alone and in combination, on the expression of mosaic symptoms in these three cultivars. Low light intensity (270-330 uE/m2/sec) significantly increased plant heights and severity of mosaic disease among the cultivars compared to high light intensity (100-200 uE/m2/sec). PVX and PVY, as well as the combination of PVX and PVY in the same plant, decreased plant height compared to the uninoculated (healthy) controls. Low light intensity and dual infections of PVX and PVY significantly increased mosaic disease severity in Shepody and Red LaSoda, but not in Russet Norkotah. Results of these studies refute the suggestion that Russet Norkotah is resistant to PVY infection since virus titers in this cultivar are similar to the known susceptible cultivars Shepody and Red LaSoda. These results further suggest that while Russet Norkotah is fully susceptible to infection by PVY, it resists symptom expression.  相似文献   

2.
Summary Potato spindle tuber viroid (PSTV) and potato virus Y (PVY) were isolated from plants of cultivar Kennebec with severe necrotic symptoms in the field. In the greenhouse, severe necrotic symptoms were reproduced only when potato plants were infected either simultaneously with PSTV+PVY, or with PSTV prior to PVY infection. Thirteen additional potato cultivars were tested in the greenhouse for this synergistic reaction and eight developed necrotic responses similar to cv. Kennebec. PVY concentration was significantly higher in doubly infected plants, compared with those infected with PVY alone.  相似文献   

3.
The ring rot bacterium,Corynebacterium sepedonicum (Spieck. and Kotth.) Skapt. and Burkh., and latent potato viruses (potato virus S and potato virus X) were investigated for their effect on ring rot symptom development on potato plants in the greenhouse and on symptom development and yield of potatoes in the field. Both virus-free (VF) and virus-infected (VI) Red Pontiac stem cuttings root-inoculated with ring rot bacteria in the greenhouse developed typical (T) ring rot symptoms, and symptom severity did not differ between VF and VI plants. In a field study, both VF and VI Russet Burbank seed pieces knife-inoculated with ring rot bacteria produced plants with atypical (A) and T ring rot symptoms as well as a combination of both types. The data suggest that more A than T symptoms develop on VI plants and more T than A symptoms develop on VF plants. Combined infection with the ring rot pathogen and the latent potato viruses resulted in greater yield losses of total and marketable Russet Burbank tubers than infection with the bacterial or viral pathogens alone.  相似文献   

4.
Field studies were conducted over a three-year period to investigate effects of potato virus X (PVX) on the Russet Burbank potato cultivar. Seed (PVX-free and PVX-infected) used for this three-year study originated from the same PVX-free source and was grown and stored under similar conditions. PVX-free seed was found to increase yield over PVX-infected plots by 9 to 32%. With these yield benefits, effects of PVX on either the incidence of verticillium wilt or plant nutrition were not significant. There was a trend (P = 0.10) for a reduction of mean tuber weight when the PVX infection level exceeded 19%. Because of increases of undersized potatoes, the yields of U.S. #1 potatoes were reduced as levels of PVX infection were increased. With a PVX infection level of 36%, the yield of U.S. #1 tubers was reduced by 21% when compared with plots having 0% PVX. At an 88% infection level, the yield of U.S. #l’s was reduced still further (36% lower than plots with 0% PVX). Results demonstrate the importance of the level of PVX infection to potato production.  相似文献   

5.
The practice of transplanting microplants from tissue-culture to the field was compared to normal tuber propagation with respect to the transmission and translocation of potato virus S (PVS) and potato virus Y (PVY) to the daughter tubers of Red Pontiac, Shepody, Kennebec, and Russet Burbank cultivars in Prince Edward Island in 1984 and 1985. In general, the use of microplants to produce seed stocks appeared to increase the risk of infection with both viruses, although the results for tuber infection with PVY were only significant in one of the years.  相似文献   

6.
Potato virus Y (PVY) infection is one of the greatest challenges to seed potato production in the United States. To determine how cultivar and seed type affect the development of systemic PVY infection, Russet Burbank and Russet Norkotah Colorado 3 cultivars were grown from two types of pre-nuclear seed (i.e., plantlets and minitubers) and Generation 3 (G3) tubers and challenged with PVY strain Wilga (PVYN-Wi). Systemic PVY infection was measured by assaying spread of virus from the inoculation site to upper non-inoculated leaves. The Burbank cultivar had a lower incidence of systemic PVY infection compared to the incidence of systemic PVY that developed in the Colorado 3 cultivar. Furthermore, Burbank plants grown from G3 tubers had a lower incidence of systemic PVY infection, as compared to Burbank plants grown from plantlets. Together our results indicate that both cultivar and seed type affect the development of systemic PVYN-Wi infections post-inoculation.  相似文献   

7.
Effects of the soil systemic insecticides aldicarb, phorate, and disulfoton on potato growth were measured from 1984–1986 on the cultivars Russet Burbank and Centennial. In all seasons, aldicarb was observed to cause a significant promotion of flowering and in one season also caused increased haulm growth. Phorate caused a reduction in these growth parameters in one season. Temporary effects on early season yields were observed from the aldicarb treatment in one year but final yields were never significantly affected by insecticide use alone. During 1985–1986 additional treatments were investigated involving insecticide combinations with the herbicide metribuzin to determine possible insecticide-herbicide interactions. Phorate-metribuzin combinations caused synergistic metribuzin injury symptoms, decreased haulm growth and yield in 1986. Metribuzin also decreased yields of aldicarb treated potatoes in 1986 and disulfoton treated potatoes in 1985. Disulfoton-metribuzin combinations decreased flowering in 1985. These data suggest that soil systemic insecticides can have direct effects on potato growth, independent of effects on pest suppression, and that they may interact with other crop protection chemicals.  相似文献   

8.
‘Alturas’, a late-maturing, high-yielding, russet potato cultivar with high tuber specific gravity, was released in 2002 by the USDA-ARS and the Agricultural Experiment Stations of Idaho, Oregon, and Washington. Originally selected for dehydration processing, its cold-sweetening resistance also makes it suitable for processing out of storage into french fries and other frozen potato products. Culinary quality is high, with larger tubers suitable for fresh market if heavily russeted skin is not essential. Alturas consistently produced greater total and U.S. No. 1 yields than ‘Russet Burbank’ and ‘Ranger Russet’ in southern Idaho trials. Across other western trial sites, total yields of Alturas have on average been 29% and 14% greater than Russet Burbank and Ranger Russet, respectively. Alturas is resistant to Verticillium wilt (Verticillium dahliae) and early blight (Alternaria solani). Compared with Russet Burbank and Ranger Russet, Alturas is less susceptible to corky ringspot and foliar and tuber infection by late blight (Phytophthora infestans). It also is less susceptible to tuber net necrosis and Fusarium dry rot than Russet Burbank, and is more resistant to common scab (Streptomyces scabies) than Ranger Russet. However, Alturas is more susceptible to infection by PVY and PVX than Ranger Russet. Total nitrogen application recommendations for Alturas are approximately 40% less than those for Russet Burbank. Alturas requires 15% to 20% more water during the growing season than does Russet Bur-bank.  相似文献   

9.
Enzyme-linked immunosorbent assay (ELISA), with single or combined antisera was effective for diagnosing potato virus S (PVS), potato virus X (PVX) or both viruses in plants grown in the greenhouse or field. In dormant tubers, stolon, middle and apical end composite sampling with or without eyes and sprouted tubers produced reliable positive assays for PVX. Only tuber pieces with sprouts resulted in consistently reliable assays for PVS. Composite sampling of potato foliage was effective for detecting one PVX infected plant in a total of 100 Kennebec, Norland, or Russet Burbank plants. There were some false negative results and greater variability in composite PVS assays, but on average, one PVS infected plant can be detected in composites of 10 Kennebec, Norland, or Russet Burbank plants. Sodium diethyldithiocarbamate (0.01M NaDIECA) in phosphate buffered saline + 0.5% Tween (PBS-T) added to plant extracts enhanced specific reactions for either virus. Onceor twiceused enzyme conjugate was effective in ELISA of either virus from potato foliage.  相似文献   

10.
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11.
Viruses that infect naturally an important Sudanese potato stock Zalinge were detected using enzyme-linked immunosorbent assay, immunosorbent electron microscopy and sap-inoculation to test plants. All of the 19 plants of Zalinge tested were infected with potato leaf roll virus (PLRV) and potato virus S (P VS), and five plants also with potato virus X (PVX). No potato virus Y (PVY), A (PVA) nor M (PVM) were found. The viruses were eradicated with thermo and chemotherapy using standard procedures. The combination of both therapies did not result in any virus-free plants, but resulted in poor plant survival. Thermotherapy reduced the incidence of PLRV and PVS by 45% and 50%, respectively, and one virus-free plant was obtained. It grew vigorously in the greenhouse, was symptomless and had a significantly increased tuber yield compared to the virus-infected plants. Following sap-inoculation with PVYO, Zalinge showed mosaic symptoms, developed necrosis in the leaves and stem and died 14 days post-inoculation. However, the plants of Zalinge infected with PVYN remained symptomless, which suggested that hypersensitivity was specific to PVYO. The fast development of lethal necrosis following infection with PVYO may contribute to the low incidence of PVY in Zalinge in the field in Sudan.  相似文献   

12.
The Agricultural Experiment Stations of Oregon, Idaho, and Washington and the U.S. Department of Agriculture jointly released Wallowa Russet in 2002. This late maturing variety is most suitable for frozen french fry use, but may find limited fresh market potential because of outstanding flavor and texture when baked. Wallowa Russet was evaluated as AO87277-6 in Oregon irrigated trials from 1993 through 1999, and in western regional trials from 1997 through 1999. Averaged over 13 locations in seven states, Wallowa Russet produced total yields of 55.6 mt/ha compared to 53.5 and 45.7 mt/ha for Russet Burbank and Russet Norkotah, respectively, and U.S. No.1 yields of 44.4 mt/ha compared to 30.7 and 36.0 mt/ha for Russet Burbank and Russet Norkotah. Specific gravity is consistently higher for Walowa Russet than for Russet Burbank and fry color is as light or lighter; sugar end development is less than that observed in Russet Burbank. Wallowa Russet is similar to Russet Burbank in susceptibility toVerticillium wilt, foliar early blight (Alternaria solani), and potato leafroll virus (PLRV). However, Wallowa Russet develops less net necrosis than Russet Burbank from PLRV infection. Wallowa Russet is more susceptible to common scab (Streptomyces scabies) than Russet Burbank. It has moderate resistance to potato virus Y (PVY). Tubers of Wallowa Russet are less susceptible to tuber infection and decay caused by potato late blight (Phytophthora infestans) than Russet Norkotah and Ranger Russet. Wallowa Russet has less hollow heart, brown center, blackspot bruise, and fewer growth cracks than Russet Burbank but is more susceptible to shatter bruise. Vitamin C is higher for Wallowa Russet (30.4 mg/100 g fresh weight) than for Russet Burbank (21.9) or Russet Norkotah (22.0), and protein content averaged 5.6% compared to 4.7% and 4.9% for Russet Burbank and Russet Norkotah, respectively.  相似文献   

13.
Collectotrichum coccodes, the pathogen causing black dot disease of potato, was investigated to determine its potential for causing symptoms on the aerial parts of potato plant, premature vine death, and reduction in yield under greenhouse and field conditions. Spray inoculations with conidial suspensions (106 colony forming units/ml) ofC. coccodes caused sunken, dark, necrotic lesions on stems, leaves and petioles, vein and leaf necrosis, dieback and premature vine death under growth chamber and greenhouse conditions. Field inoculations in 1986 and 1987 provided evidence for an increased incidence of premature vine death in seven potato genotypes (Russet Burbank, Norgold Russet, Superior, Butte, NDA 8694-3, A68113-4 and A66107-51). Late season application of metribuzin at 0.84 kg/ha significantly increased the incidence of stems with black dot symptoms in cv. Russet Burbank at one location. Field inoculations in 1987 at two locations resulted in yield reduction in the late-maturing cv. Russet Burbank (6.3 and 6.5 t/ha) and clone A68113-4 (5.0 t/ha), but not in the early maturing cv. Norgold Russet. Similar results were obtained in greenhouse studies following soil inoculations withC. coccodes: yield reduction was observed in cv. Russet Burbank but not in cvs. Norgold Russet, Superior and Katahdin. Colonization of stem ends of tubers byC. coccodes from spray inoculated field plots was higher than in tubers from uninoculated plots at one of the two locations. These results point to a much broader potential ofC. coccodes as a potato pathogen, than hitherto documented. Colletotrichum coccodes (Wallr.) Hughes (syn.C. atramentarium (Berk. & Br. Taub.) was described in 1825 (21) and has a wide host range (5). Potato and tomato are the more economically important hosts. This fungus has been variously referred to as an unspecialized, minor pathogen or secondary invader (18), weak pathogen (22), specialized parasite (5), or superficial colonizer and widespread epiphyte (19). The symptoms produced by this pathogen on potato have been described as black dot (10, 14) on stems, stolons, tubers and roots, sometimes associated with leaf scorch or wilt (24), rubbery tuber wilt (1), and tuber skin necrosis (17). The fungus is commonly associated with senescent, decaying root and stem tissue, especially at the end of the season. In Idaho, instances of premature death of potato without typical symptoms of Verticillium wilt, but accompanied by a high incidence of stems with black dot symptoms, have been observed in potato crops grown on fields fumigated with metam sodium. Additional observations by growers have also implicated the possible interaction of the herbicide metribuzin with the incidence of black dot. Although it was observed by some workers to accelerate leaf senescence (12, 22), Schmiedeknecht (21) concluded thatC. coccodes was unable to infect the green leaves of potato plant. Even thoughVerticillium dahliae is generally considered the major factor in potato early dying (20), several workers (7, 11, 13, 19, 22) have investigated the possible role ofC. coccodes in causing premature death of potato (8). However, these studies examinedC. coccodes only as a soilborne, root and tuber pathogen of potato; its effects on shoot portions (aerial parts) of the plant were not considered. In addition to causing anthracnose of fruits of tomato, pepper and squash (18), recent reports indicate thatC. coccodes causes foliage blights on eastern black nightshade (2) and velvetleaf (25). The objectives of this study were: (1) to examine the potential ofC. coccodes to cause symptoms on aerial parts of potato plant, (2) to investigate the effect of spray inoculation in the field withC. coccodes on incidence of premature vine death, tuber colonization and yield of potato, and (3) to document the effect of soil inoculation withC. coccodes alone or in combination withV. dahliae on potato varieties under greenhouse conditions.  相似文献   

14.
Bannock Russet, a late-maturing, long russet potato cultivar, was released in 1999 by the USDA-ARS and the experiment stations of Idaho, Oregon, and Washington. It is suitable for processing into french fries and other frozen products. Its attractive russeted skin and excellent culinary qualities also make it suitable for fresh market. Bannock Russet, in comparisons with Russet Burbank, has consistently produced greater U.S. No. 1 yields in trials conducted throughout southern Idaho. In other western trial sites, total yields of Bannock Russet have been comparable to or smaller than that of Russet Burbank, but its percentage of undersize and cull potatoes is consistently less. Bannock Russet is resistant to potato virus Y (PVY), Verticillium wilt, common scab, and leafroll net necrosis. It also has moderate resistance to early blight (foliar and tuber) andErwinia soft rot. With respect to other potato diseases/disorders, Bannock Russet’s level of susceptibility is similar to that of Russet Burbank; the exception being shatter bruise, with Bannock Russet displaying a greater susceptibility. Cultural management guidelines have been developed to minimize shatter bruise in Bannock Russet. Bannock Russet’s multiple disease resistances coupled with its low nitrogen requirement, make it a low-input cultivar that could significantly reduce growers’ production costs.  相似文献   

15.
将多种病毒的有效核酸片断拼接成融合基因转入马铃薯可获得多抗马铃薯材料。针对马铃薯生产中分布广泛、危害严重并经常混合感染的马铃薯X病毒(PVX)、马铃薯Y病毒(PVY)、马铃薯卷叶病毒(PLRV)和马铃薯S病毒(PVS),开展了利用基因工程方法获得兼抗4种马铃薯病毒转基因马铃薯材料的研究。试验在前期获得含4种马铃薯病毒外壳蛋白基因片段的质粒pART27-XSYV-rh的基础上,通过根癌农杆菌(Agrobacterium tumefaciens)介导转化马铃薯(Solanum tuberosum)品种‘陇薯3号’,PCR扩增和PCR-Southern杂交证明,4价融合基因已整合到马铃薯基因组中。qRT-PCR分析表明,该融合基因在转基因植株中能正常表达。3株转基因植株的抗病性鉴定结果表明,2株对4种病毒同时具有抗性;1株对PLRV侵染表现阳性,对另外3种病毒同时具有抗性。  相似文献   

16.
Umatilla Russet, a moderately late maturing variety especially suitable for frozen French fry processing but also acceptable for tablestock use (boiling, and baking), was jointly released by the Agricultural Experiment Stations of Oregon, Idaho, and Washington and the U.S. Department of Agriculture in 1998. Umatilla Russet was evaluated in irrigated trials in Oregon from 1988 to 1997, and in western regional trials from 1989 to 1991. Total yields were similar to those of Russet Burbank, but Umatilla Russet produced higher marketable yields. In three years of regional trials at up to 13 locations in seven western states, Umatilla Russet averaged 40.5 Mg/ha compared with 32.4 Mg/ha for Russet. Burbank. Umatilla Russet fry color and specific gravity are consistently equal to or better than for Russet Burbank. Umatilla Russet is less susceptible toVerticillium wilt than Russet Norkotah, less susceptible to net necrosis than Russet Burbank, and resistant to PVX. It is susceptible to PLRV and expresses foliar symptoms of PVY more clearly than Russet Norkotah. Umatilla Russet is less susceptible to tuber infection and decay caused byPhytophthora infestans than Ranger Russet and Russet Norkotah. Umatilla Russet is less susceptible to hollow heart, brown center, growth cracks, and sugar ends, but more susceptible to blackspot and shatter bruise than Russet Burbank  相似文献   

17.
本文分别在温室和田间条件下利用人工接种的方法研究了马铃薯纺锤块茎类病毒（ＰＳＴＶ）和ＰＶＹ在克新４号及Ｋｅｎｎｅｂｅｃ两品种上的相互作用．试验结果表明：在温室条件下ＰＳＴＶ和ＰＶＹ的复合接种均能在二品种上不同程度地产生条型坏死症状，而在田间未出现明显症状．ＰＳＴＶ与ＰＶＹ混合接种或ＰＳＴＶ接种一周后再接ＰＶＹ会促进ＰＶＹ增殖，植株内ＰＶＹ的浓度显著高于单独接种ＰＶＹ，但ＰＶＹ侵染后再接ＰＳＴＶ对ＰＶＹ的浓度无显著影响．ＰＳＴＶ和ＰＶＹ的工作对ＰＳＴＶ的浓度无显著影响．ＰＳＴＶ与ＰＶＹ复合侵染的三个接种类型都明显地降低平均单株产量和商品薯率，产量损失最大的处理为ＰＳＴＶ与ＰＶＹ混合接种，可导致克新４号减产４５．４％。  相似文献   

18.
Summary Greenhouse experiments demonstrated a differential interaction between potato viruses X and Y in two potato cultivars. Enhancement of PVX synthesis in doubly infected plants occurred only in the PVY-susceptible potato cv. Ulla, whereas PVX multiplication was almost completely inhibited in the doubly infected PVY-resistant cv. Franzi. However, a synergistic effect was also evident in the latter cultivar in the form of a growth inhibition of the plants. An increased multiplication of one or both of the viruses is, therefore, not related to a synergistic reaction.  相似文献   

19.
The survival ofCorynebacterium sepedonicum, the bacterial ring rot pathogen, in infected potato stems and on burlap surfaces held at various freezing and above-freezing temperatures was investigated by root-inoculating potato stem cuttings with aqueous suspensions prepared from these materials. Infectious bacteria were recovered from dried Russet Burbank stems held for 44 mo in an unheated machine shed and from dried Warba stems held for 55 mo. Inoculum from dried stems of all cultivars held for 63 mo did not incite symptoms; however, inoculum from Russet Burbank stems incited symptomless infection in 10% of the inoculated plants. This 63-mo survival period exceeds a 26-mo survival period previously reported. Ring rot bacteria survived and remained infectious for at least 18 mo on burlap surfaces subjected to temperatures of ?40° to ?5°C, alternating temperatures of -5° to 5°C, or a constant temperature of 5°C. Inoculum from burlap stored at the lower temperatures caused the most severe symptoms. These results stress the need for keeping crop debris away from potato operations and for using proper decontamination procedures to maintain potato seed stocks free of ring rot.  相似文献   

20.
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