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不同栽培基质对番茄植株长势、果实品质及产量的影响 总被引：3，自引：0，他引：3
试验以土壤、菇渣:砂=3:1和菇渣:砂:鸡粪=4:2:1为栽培基质,采用定期浇灌营养液的方式来栽培番茄,研究了不同栽培基质对其植株长势、果实品质及产量的影响。结果表明:与土壤相比,菇渣:砂=3:1和菇渣:砂:鸡粪=4:2:1基质栽培有利于株高生长和茎的增粗,但对叶绿素含量影响不大;菇渣:砂=3:1的植株地上部和地下部全氮和全磷含量均显著高于其它处理;土壤的植株地上部和地下部全钾含量均显著高于其它处理;单果重和单株平均产量以菇渣:砂:鸡粪=4:2:1最高;糖酸比由高到低的顺序为:菇渣:砂:鸡粪=4:2:1>菇渣:砂=3:1>土壤,VC含量由大到小的顺序为:菇渣:砂=3:1>菇渣:砂:鸡粪=4:2:1>土壤。综合3种栽培基质对番茄植株生长、果实品质和产量等指标的影响,菇渣:砂:鸡粪=4:2:1为番茄基质栽培的最优组合。 相似文献
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不同基质栽培对番茄叶片光合作用和根系ATP酶的影响 总被引：1，自引：0，他引：1
以腐熟玉米秸、麦秸、菇渣、锯末等农产废弃物添加土壤后配成有机基质进行番茄栽培试验,探讨不同基质栽培对番茄叶片光合特性和根系ATP酶活性的影响。结果表明,有机基质栽培较土壤栽培植株同化产物积累增加,番茄光合速率、细胞间隙CO2浓度、呼吸速率均显著提高。有机基质栽培Fv/Fm、ΦPSⅡ、qP显著升高,P-H++ATPase、P-Ca2+-ATPase、V-H+-ATPase、V-Ca2+-ATPase活性提高。有机基质栽培番茄通过提高根系质膜和液泡膜ATPase活性、增加番茄的光合速率、细胞间隙CO2浓度,提高Fv/Fm、ΦPSⅡ、qP,提高光化学效率,增强营养吸收,从而促进番茄生长和同化物形成。 相似文献
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以粗砂、蛭石、菇渣、树叶、锯末为基质材料,不同材料按体积比复配后形成10种复混基质,以粗砂基质为对照,进行"欧曼"品种番茄栽培试验。采用相同尺寸栽培槽填充基质、无土栽培水肥管理及田间整枝管理措施,研究了不同配比基质理化性质、根区温度与根区湿度,及其对番茄果实品质及产量的影响。结果表明:复混处理后基质的容重较CK显著降低,孔隙度较CK显著升高;复混基质能改善根区的温湿环境,不同处理平均温度较CK提高了0.35~1.24℃,平均湿度较CK提高1.86%~13.32%;对比果实产量和品质发现T6与T8处理总体表现最好,果实硬度、单果质量、单株产量、单株结果数、增产率均优于其它处理,维生素C、可溶性蛋白质、有机酸含量也均处于较好水平,综合比较发现T6处理(粗砂∶蛭石∶菇渣∶树叶∶锯末=2.5∶2.5∶1∶2∶2)和T8处理(粗砂∶蛭石∶菇渣∶树叶∶锯末=2.5∶2.5∶1∶1∶3)能为番茄生长提供良好生长环境,并能提高番茄的产量及品质。 相似文献
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醋糟和锯末基质对温室黄瓜生长的影响 总被引：1，自引：0，他引：1
以黄瓜为试材,醋糟和锯木屑作为2种主要基质原料,通过设定醋糟、锯木屑和土壤等的不同配比(纯醋糟C、纯锯末J、醋糟∶土=4∶1CT、锯末∶土=4∶1JT、醋糟∶锯末=2.5∶2.5CJ、醋糟∶锯末∶土=2∶2∶1CJT),对比分析基质处理对黄瓜株高、茎粗、叶片数等生长指标及产量的影响,为醋糟和锯末作为设施黄瓜栽培基质的适用性提供理论依据。结果表明:醋糟与锯末等不同配比的各基质处理均具有良好的孔隙度、pH、EC等理化性状,纯醋糟以及添加醋糟比例较高的基质处理还含有较丰富的有效养分含量;不同基质处理对黄瓜生长的影响差异较大,纯醋糟以及添加醋糟的基质处理(C、CT、CJT)可明显促进黄瓜的株高、茎粗的增长和叶片数的增加,而添加锯末的基质处理(J、JT)生长较差,纯锯末处理生长最差;黄瓜单株产量以C和CT处理最高,J处理最低。综合以上结果,C和CT基质处理栽培的黄瓜株高、茎粗、叶片数及果实产量都明显优于草炭和土壤对照,可以作为草炭的替代栽培基质。 相似文献
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AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation. 相似文献
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AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration. 相似文献
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《International Journal of Fruit Science》2013,13(3):101-120
Abstract Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared. 相似文献
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The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species. 相似文献
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多效唑对猕猴桃离体试管苗生长及内源激素的影响 总被引：18，自引：0，他引：18
多效唑（ＰＰ３３３）处理猕猴桃试管苗，降低了其生长强度；植株体内的ＧＡ３、ＩＡＡ和ＺＴ含量下降，ＡＢＡ的含量上升，乙烯释放率增加；并且能降低外源的ＧＡ３和ＩＡＡ促进生长的作用，而外源的ＧＡ３和ＩＡＡ又能不同程度地逆转多效唑的抑制作用，使植株恢复生长。 相似文献
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HU Zhi-cheng ZHU Jia-yuan ZHU Bin GUO Dong CHEN Bin ZHANG Kai HU Kun-hua LI Ming-tao TANG Bing 《园艺学报》2011,27(9):1802-1806
AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars. 相似文献
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AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation. 相似文献
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Duncan Brean W. Boyle Shannon Breininger David R. Schmalzer Paul A. 《Landscape Ecology》1999,14(3):291-309
Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere. 相似文献
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AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis. 相似文献
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XUE Ying-sheng WANG Jing-feng NIE Ru-qiong WU Wei-kang LUO Han-chuan CHEN Xiao-wei 《园艺学报》2004,20(4):603-607
AIM: To investigate the influence of Sini decoction (SND) on the proliferation and apoptosis of rabbit abdominal aorta smooth muscle cells after ballon injury and discuss the effect of vascular smooth muscle cell's (VSMCs) proliferation and apoptosis in post-percutaneous coronary intervention (PCI) restenosis (RS) and the feasibility of SND preventing post-PCI RS. METHODS: The animal model of rabbit abdominal aorta ballon injury was set up and the therapertic group was treated with SND. The shape of proliferative and apoptotic cell were investigated by electron microscope. Immunohistochemistry staining was performed using α-actin,PCNA and Cyclin E monoclonal antibodies. In situ Cell Death Detection Kit was used to identify apoptotic cells. Abdomial aorta angiography was operated in the 84th day subgroup and the stenosis degree was evalued by quantitative angiographic analysis. RESULTS: As compared with the control group, the therapeutic group displayed a lower proliferative percentage and a higher apoptosic percentage (P<0.05). Moreover, the apoptosic peek time was on the 14th day after operation,which was longer than the control group. CONCLUSION: SND effectly inhibited the proliferation of VSMCs and iuduced apoptosis in VSMCs. 相似文献