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1.
Glyphosate ? ? Mention of irademark or proprietary product does not constitute a gtiarantee or warranty oC the product by the U.S. Department of Agriculture and does nut imply its approval to the exclusion of other products thai may also be suitable.
was sprayed at 0009–1·12 kg a.i. ha?1 on the foliage of large potted glasshouse-grown Canada thistle [Cirsium arvense (L.) Scop.], which had extensive, well-developed roots. Increasing the glyphosate rate progressively reduced the total number of visible adventitious root buds plus emerged secondary shoots per plant proportionately more than root biomass, 10 days after treatment. Cortical tissue of thickened propagative roots became soft, water-soaked, darkened, and some regions decomposed, exposing strands of vascular tissue. Lateral roots completely decomposed. When thickened roots were segmented to stimulate secondary shoot emergence from root buds 10 days after foliar treatment, Fewer secondary shoots emerged than expected from the number of visible adventitious root buds present on both control and herbicide-treated plants. Increasing the rate of glyphosate also reduced the regrowth potential of root buds proportionately more than root biomass. Regrowth potential was measured as the number of emerged secondary shoots 35 days after segmenting unearthed roots from plants that had been sprayed 10 days earlier. When foliar-applied at 0·28 kg ha?1, glyphosate decreased the regrowth potential of root buds to zero in 2 and 3 days, as measured by secondary shoot dry weight and number, respectively, even though root fresh weight was unchanged 3 days after foliar treatment. These dose-response and time-course experiments demonstrate that glyphosate did not reduce root biomass as much as it decreased root bud numbers and secondary shoot regrowth potential from root buds.  相似文献   

2.
The pattern and extent of 14C-glyphosate [N-(phosphonomethyl)glycine] translocation from the treated leaf and metabolism of 14C-glyphosate were studied in field bindweed (Convolvulus arvensis L.), hedge bindweed (Convolvulus sepium L.). Canada thistle [Cirsium arvense (L.) Scop.] tall morning glory [lpomoea purpurea (L.) Roth.] and wild buckwheat (Polygonum convolvulus L.). 14C was translocated throughout the plants within 3 days with accumulation in the meristematic tips of the roots and shoots evident. Cross and longitudinal sections of stems and roots showed that the 14C was localized in the phloem. Field bindweed translocated 3–5% of the applied 14C from the treated leaf, hedge bindweed 21.6%, Canada thistle 7.8%, tall morningglory 6.5%, and wild buckwheat 5%. Field bindweed, Canada thistle, and tall morningglory metabolized the parent glyphosate to aminomethylphosphonic acid to a limited extent. This metabolite made up less than 15% of the total 14C. Of the total 14C applied to excised leaves, 50% had disappeared within 25 days.  相似文献   

3.
在人工气候室培养空心莲子草,植株经草甘膦与乙烯利混合处理后,测定对植株的抑制作用和草甘膦的吸收与传导量。结果表明,加入乙烯利(100mg·L~(-1))后草甘膦(300mg·L~(-1))对地下根茎抑制率比对照提高了13.6个百分点。植株经乙烯利喷雾处理后,基芽、地下茎和根系中~(14)C-草甘膦含量分别是对照的3.56、1.75和2.35倍。放射性成像图显示,植株地下茎与根系中~(14)C-草甘膦传导量明显高于对照。  相似文献   

4.
The transport and differential phytotoxicity of glyphosate was investigated in maize seedlings following application of the herbicide to either roots or shoots. One-leaf maize seedlings (Zea mays L.) were maintained in graduated cylinders (250 mL) containing nutrient solution. Half of the test plants were placed in cylinders (100 mL) containing different 14C-glyphosate concentrations; the remainder received foliar appliation of 14C-glyphosate. After 26 h, the roots and the treated leaves were washed with distilled water, and the plants placed again in cylinders (250 mL) containing fresh nutrient solution for 5 days. Plants were weighed, and split into root, seed, cotyledon, coleoptile, mesocotyl, first leaf and apex. The recovery of 14C-glyphosate was over 86%. For both application treatments, the shoot apex was the major sink of the mobilized glyphosate (47.9 ± 2.93% for root absorption and 45.8 ± 2.91% for foliar absorption). Expressed on a tissue fresh weight basis, approximately 0.26 μg a.e. g−1 of glyphosate in the apex produced a 50% reduction of plant fresh weight (ED50) when the herbicide was applied to the root. However, the ED50 following foliar absorption was only 0.042 μg a.e. g−1 in the apex, thus maize seedlings were much more sensitive to foliar application of the herbicide.  相似文献   

5.
Experiments were conducted in a growth cabinet to investigate the absorption and translocation of 14C-3, 6-dichloropicolinic acid by Cirsium arvense (L.) Scop. (Canada thistle, creeping thistle), a sensitive species. Applications were made, either to the middle four leaves of 12-cm-tall vegetative plants grown under low (40%) and/or high (>95%) relative humidity (r.h.), or to four upper or lower leaves of 30-cm-tall flowering plants grown under low r.h. Following application to vegetative plants, absorption and translocation of 14C-3,6-dichloropicolinic acid was rapid and was approximately doubled by high r.h. High r.h. increased the amount of radioactivity retained by the treated leaves or translocated to the shoots but did not affect greatly the amount retained in the roots. The herbicide was highly mobile, with over half of that absorbed, translocated out of the treated leaves after two days. The apex accumulated most of the radioactivity, while approximately 8% was recovered from the roots. The absorption and translocation patterns were similar to those reported in the literature for picloram in C. arvense. Absorption of 3,6-dichloropicolinic acid was greater in vegetative than in flowering C. arvense plants, and placement of herbicide on lower leaves tended to decrease the amount of radioactivity recovered from shoot apex and increase the amount recovered from the roots. Approximately 15% of the applied radioactivity could not be recovered from treated plants by 2 days after treatment.  相似文献   

6.
Absorption, translocation and distribution of 14C-glyphosate were examined in Agropyron repens (L.) Beauv. plants growing under field conditions in the autumn. Glyphosate absorption did not increase beyond 3 days after application, whereas translocation to the rhizomes continued up to 7 days after application. The translocated glyphosate accumulated more in new rhizomes than in older parts of the rhizomes. Ten per cent of the glyphosate translocated out of the treated shoot was recovered in younger shoots 7 days after application. Plants harvested the following spring contained less than 20% of the glyphosate originally applied. Although a growth cabinet experiment indicated that 34% of the glyphosate in the rhizomes of treated plants could be remobi-lized into new aerial shoots, considerably less was recovered in new, aerial shoots in the spring in the field-grown plants. Freezing experiments showed that glyphosate translocation to the rhizomes was only prevented when cold treatment caused visible damage to A. repens foliage.  相似文献   

7.
Various control strategies for Achillea millefolium L. (yarrow) were investigated in a dense stand of the weed at Lincoln College in 1977–1978. In early spring plots were either rotary cultivated or left undisturbed. In late spring, plots of both previous treatments were either left undisturbed, rotary cultivated or sprayed with glyphosate at 1·5 kg ha?1. The whole experiment was rotary cultivated twice 1 week later and sown with Hordeum vulgure L. cv. Zephyr (barley) at 144 kg seed ha?1. MCPA + dicamba at 0·9+0·15 kg ha?1 was applied to half of each plot when the second node was detectable (Zadok 32). Rotary cultivation and glyphosate both substantially reduced the regrowth of A. millefolium but glyphosate reduced regrowth by a greater proportion when applied to undisturbed plants than when applied to plants regenerating after cultivation. Both gave a more than 95% reduction compared to the control (rotary cultivation only at sowing time) in the amount of A. millefolium present in the barley stubble in the autumn. MCPA + dicamba caused seedling mortality but did not affect the numbers of primary shoots from rhizome fragments. The grain yield of the barley increased from 2·91 t ha?1 when A. millefolium was not controlled to 4·23 t ha?1 with good control. The barley yield appeared to be restricted by competition from regenerating A. millefolium and by a nitrogen deficiency induced in some regimes by nitrogen immobilization in decaying rhizomes.  相似文献   

8.
The uptake, translocation and metabolism of glyphosate [N-(phosphonomethyl) glycine] by Canada thistle (Cirsium arvense (L.) Scop.) (susceptible) and leafy spurge (Euphorbia esula (L.)) (resistant) were examined in an attempt to elucidate the nature of the differential sensitivity. The pattern of uptake and translocation was similar in both species. Glyphosate moved readily in the apoplast and the symplast. High humidity and/or surfactant greatly increased the amount of 14C-glyphosate absorbed and translocated over that in low humidity and/or without surfactant. No 14Cmetabolites were detected in either species 1 week after treatment with 14C-glyphosate. More of a glyphosate spray solution containing a fluorescent dye was received and retained on Canada thistle by virtue of its growth habit than on leafy spurge. More glyphosate should therefore be available for uptake by Canada thistle and this may account for the differential sensitivity of these two species.  相似文献   

9.
The non-ionic surfactants, Tween 20 (polyoxy-ethylene 20 sorbitan monolaurate) and Renex 36 (polyoxyethylene 6-tridecyl ether) enhanced the retention of a glyphosate-dye spray solution by barley (Hordeum vulgare L.) leaves. Tween 20 also enhanced absorption of 14C-glyphosate applied as droplets to barley leaves whereas Renex 36 similarly applied, reduced both absorption and movement of 14C-glyphosate. Renex 36 alone or mixed with glyphosate increased leakage of electrolytes from barley leaf segments whereas neither Tween 20 nor glyphosate, alone or mixed together, had any effect. No 14C-glyphosate complexes were detected in mixtures with either surfactant and neither surfactant affected the pH of the glyphosate solution. The results indicate that the reported enhancement of glyphosate phytotoxicity by Tween 20 is due to increased retention and absorption of the herbicide while the reported antagonism caused by Renex 36 is due to reduced glyphosate absorption and movement possibly as a result of alteration of membrane integrity.  相似文献   

10.
Field and glasshouse experiments were conducted from 1995 through 1996 to evaluate application timing of asulam (methyl sulfanilylcarbamate) for torpedograss (Panicum repens L.) control in relation to plant age in sugarcane. Above‐ground shoots of torpedograss were completely controlled with asulam at 2–4 kg active ingredient (a.i.) ha?1 applied 60 or 80 days after planting (DAP) in artificially infested pots. But some newly developed rhizome buds survived after asulam application resulting in 1–25 and 76–100% or more regrowth in 60 and 80 DAP‐applied pots, respectively. Whereas the herbicide at 2–4 kg a.i. ha?1 applied within 60 DAP completely controlled above‐ground shoots, applied 80 DAP at 2 kg a.i. ha?1 it did not completely control the weed in the artificially infested field. Regrowth levels were 1–25 and 76–100% or more in 60 and 80 DAP‐applied plots, respectively. Asulam at 2–3 kg a.i. ha?1 applied 20, 40, 60 or 80 DAP in a naturally infested field completely controlled above‐ground shoots and regrowth levels were 76–100 or more, 51–75, 1–25 and 26–50% in these same DAP applied plots, respectively. The herbicide applied at 4 kg a.i. ha?1 caused chlorosis on younger sugarcane leaves (one‐leaf stage), but when applied at 2–3 kg a.i. ha?1, no injury symptoms were shown. The herbicide at 2–4 kg a.i. ha?1 applied within 60 DAP resulted in remarkably higher yield and shoot biomass of sugarcane than that applied 80 DAP. This study suggested that asulam at 2–3 kg a.i. ha?1 should be applied 60 days after planting for the maximum control of torpedograss regrowth and better yield of sugarcane. This study also indicated that torpedograss cannot be completely controlled with a single application of asulam in a naturally infested field because of rhizome fragmentation by cross plowing and distribution of rhizomes into different soil layers that require different times to emerge. The shoots emerging after asulam application could not be controlled. Another study is required to determine the interval between sequential applications of asulam for better control of torpedograss in a naturally infested field.  相似文献   

11.
The broomrape (Orobanche crenata Forsk) susceptibility to glyphosate applied on faba-bean (Vicia faba L.) as affected by the parasite growth stages at the time of application was studied under field conditions. Glyphosate treatments delayed O. crenata emergence. Single glyphosate application to faba-bean infected with O. crenata predominantly at the stage (a) (small nodule) and (b) (nodules with initial vestigial roots) resulted in a moderate to low control. When the stages (c) (shoot bud already visible) or (d) (shoot and vestigial roots well developed) were the predominant stages, an excellent control was achieved with a single glyphosate application at 60 g ha?1. Increased development stages, with the shoot emerged from the shoot bud, decreased its susceptibility to glyphosate. La lutte contre Orobanche crenata en culture de férerole avec le glyphosate; influence des doses d'herbicide et du stade de développement du parasite  相似文献   

12.
In 1997 and 1998, five field studies were conducted at four Portuguese wine‐growing regions in order to evaluate the effectiveness of the chemical control of vineyard weeds under Mediterranean conditions using either reduced doses of residual herbicides or only foliar herbicides. Amitrole (3440 g a.i. ha?1), amitrole + glyphosate mono‐ammonium salt (1720 + 900 g a.i. ha?1), amitrole (3400 g a.i. ha?1), amitrole + diuron (2580 + 1500 g a.i. ha?1), amitrole + simazine (2580 + 1500 g a.i. ha?1), amitrole + terbuthylazine (2580 + 1500 g a.i. ha?1) and amitrole + diuron + simazine (2580 + 1300 + 1400 g a.i. ha?1) were assayed and compared with the following reference herbicides: glyphosate isopropylamine salt (1800 g a.i. ha?1), amitrole + diuron (2520 + 1680 g a.i. ha?1), diuron + glyphosate + terbuthylazine (1275 + 900 + 1425 g a.i. ha?1), amitrole + simazine (1900 + 3900 g a.i. ha?1) and glyphosate + simazine (800 + 2200 g a.i. ha?1). The herbicides were applied during late winter. The results indicated that good control was achieved by the application of foliar herbicides alone or of reduced rates of a mixture of residual herbicides with foliar herbicides for at least 2 months. Three months after application, the efficacy of post‐emergence herbicides and lower rates of residual herbicides decreased significantly in clay soils and under heavy rainfall conditions. Convolvulus arvensis– a weed that is becoming increasingly significant in Portuguese vineyards – was poorly controlled, even when glyphosate was used. Despite this, it can be assumed that in those regions in which the trials were conducted, it is possible to employ weed control strategies that entail the elimination or a reduction in the rate of residual herbicides.  相似文献   

13.
The differential tolerance of resislant creeping red fescue (Fes- tuca rubra L, var, rutra) and susceptibie reed canarygrass (Pha- laris arundinacea L.) seedlings to glyphosate [N-(phosphono- meihyDglycine) was confirmed under growth chamber condilions. The absorption, transiocation and metabolism of 14C-glyphosate was examined in both species to determine if differences in these processes could account for the observed selectivity, Creepmg red fescue actually absorbed more glyphosate than did t-eed canarygrass, and both species rapidly translocated the herbicide throughout their respective tissues. No metabolism of glyphosate was detected in either species. Differential interception and retention of the glyphosate spray can probably be eliminated as possible selectivity mechanisms. The relative tolerance of creeping red fescue to glyphosale appears to be related to its ability lo regenerate roots and shoots from the crown of the plant, but the mechanism of resistance remains obscure.  相似文献   

14.
Li P  He S  Tang T  Qian K  Ni H  Cao Y 《Pest management science》2012,68(2):170-177
BACKGROUND: Glyphosate is a non‐selective, foliar‐applied, systemic herbicide that kills weeds by inhibiting the synthesis of 5‐enolpyruvylshikimate‐3‐phosphate synthase. Urea phosphate (UPP), made by the reaction of urea with phosphoric acid, was applied as an adjuvant for glyphosate in this study. Experiments in the greenhouse and the field were conducted to determine the effects of UPP by comparing the efficacies of glyphosate plus UPP, glyphosate plus 1‐aminomethanamide dihydrogen tetraoxosulfate (AMADS) and Roundup. RESULTS: The optimum concentration of UPP in glyphosate solution was 2.0% when UPP was used as an adjuvant. The ED50 values for glyphosate‐UPP were 291.7 and 462.4 g AI ha?1 in the greenhouse and the field respectively, while the values for Roundup were 448.2 and 519.6 g AI ha?1. The ED50 values at 2 weeks after treatment (WAT) and 3 WAT were lowered when UPP was used as an adjuvant in the greenhouse and field study, and the glyphosate + UPP was absorbed over a 2 week period. UPP may increase the efficacy by causing severe cuticle disruption or accelerating the initial herbicide absorption. The result also showed that UPP could reduce the binding behaviour of Ca2+ to glyphosate. CONCLUSION: The application of UPP as an adjuvant could increase the efficacy of glyphosate and make it possible to achieve effective control of weeds with glyphosate at lower dose. Moreover, UPP showed less causticity to spraying tools and presented less of a health hazard. Therefore, UPP is accepted as being a new, effective and environmentally benign adjuvant for glyphosate. Copyright © 2011 Society of Chemical Industry  相似文献   

15.
The rapid range expansion of naturalized Italian ryegrass (Lolium multiflorum Lam.) in farmland is a serious problem in Fukuroi city in Shizuoka Prefecture, Japan. Glyphosate has been used to control Italian ryegrass in the levees of rice paddy fields and wheat fields for ~20 years, but this weed in Fukuroi city is poorly controlled by glyphosate. In order to elucidate the level of resistance to glyphosate in Italian ryegrass populations, seed bioassays and a foliar application experiment, using seeds collected from 16 wild populations in and around Fukuroi city and from three susceptible cultivars, were conducted. For the susceptible cultivars and one population from a site where glyphosate had not been applied for >10 years, the shoot length in the seed bioassays was greatly suppressed at a glyphosate concentration of 10 mg ai L?1 and no seedling survived after the foliar application of glyphosate at a rate of 2.3 kg ai ha?1. Nine wild populations from levees in the southern part of Fukuroi city showed vigorous shoot growth at a glyphosate concentration of 10 mg ai L?1 and had at least a 78% survival rate after the application of glyphosate at 2.3 kg ai ha?1. Four wild populations from levees in the northern part of Fukuroi city showed a slight suppression of the shoot growth as a result of the glyphosate treatment and their survival rates ranged from 20 to 64%. The results suggested that resistance to glyphosate has evolved in the wild populations of Italian ryegrass that are growing on the levees. This is the first report of a glyphosate‐resistant weed in Japan.  相似文献   

16.
Many herbicides promote plant growth at doses well below the recommended application rate (hormesis). The objectives of this study were to evaluate glyphosate‐induced hormesis in soyabean (Glycine max) and determine whether pre‐treating soyabean seedlings with low doses of glyphosate would affect their response to subsequent glyphosate treatments. Seven doses (1.8–720 g a.e. ha?1) of glyphosate were applied to 3‐week‐old seedlings, and the effects on the electron transport rate (ETR), metabolite (shikimate, benzoate, salicylate, AMPA, phenylalanine, tyrosine and tryptophan) levels and dry weight were determined. The lowest dose stimulated ETR and increased biomass the most. Benzoate levels increased 203% with 3.6 g a.e. ha?1 glyphosate. Salicylate content and tyrosine content were unaffected, whereas phenylalanine and tryptophan levels were increased by 60 and 80%, respectively, at 7.2 g a.e. ha?1. Dose–response curves for these three amino acids were typical for hormesis. In another experiment that was replicated twice, soyabean plants were pre‐treated with low doses of glyphosate (1.8, 3.6 or 7.2 g a.e. ha?1) and treated with a second application of glyphosate (1.8, 3.6, 7.2, 36, 180 or 720 g a.e. ha?1) 14 days later. For total seedling dry weight, a 3.6 and 7.2 g a.e. ha?1 glyphosate dose preconditioned the soyabean seedlings to have greater growth stimulation by a later glyphosate treatment than plants with no preconditioning glyphosate exposure. Optimal hormetic doses were generally higher with pre‐treated plants than plants that had not been exposed to glyphosate. Thus, pre‐exposure to low doses of glyphosate can change the hormetic response to later low‐dose exposures.  相似文献   

17.
Broadcast sprays of several herbicides were applied at different times of the year at several sites in Hawkes Bay, Canterbury and Otago. For good thistle control, date of application was more important than types of herbicide. In Hawkes Bay, applications made in April, May and June tended to be the most effective. Under slightly cooler Canterbury conditions, April, September and October were the best application dates. In the Otago trials, spring emergence of thistle seedlings meant that the most consistent results came from September or October applications. At all sites, applications made in July or August were relatively ineffective, probably because of low winter temperatures and slow thistle growth rates. MCPA (potassium salt) at 1·0 kg ha?1 was the standard herbicide used in all experiments. MCPA at 0·5 kg ha?1, MCPB (sodium salt) at 0·5 and 1·0 kg ha?1 and 2,4-D at 0·5 kg ha?1 did not kill as many thistles as MCPA at 1·0 kg ha?1. MCPA at 1·5 kg ha?1 and MCPB (butyl) ester + clopyralid at 0·5 + 0·015 or 1·0+0·03 kg ha?1 gave consistently better control than MCPA at 1·0 kg ha?1 2,4-D at 1·0 or 1·5 kg ha?1, MCPB at 1·5 or 2·0 kg ha?1, and MCPA + MCPB at 0·33 + 1·0 or 0·67 + 0·5 kg ha?1 gave results very similar to MCPA at 1 kg ha?1. Thistle control varied between sites and years. Some of the variation may have been due to different proportions of first and second year thistles present at spraying, and to variation in genetically determined herbicide susceptibility. Chemical control of thistles was short term only, because of dormant seeds in the soil.  相似文献   

18.
The weed species, prickly sida (Sida spinosa L.) and sicklepod (Senna obtusifolia L.), were treated with 14C-glyphosate alone and formulated with different polyethlylane oxide (PEO) surfactants in tallow amine ethoxylate and non-ionic alkoxylate series to determine the amount of 14C-glyphosate absorption and translocation. The surface tension, contact angle, and 14C-glyphosate distribution were significantly affected by both the presence of different waxes on the plants and by the addition of surfactants to the glyphosate. The surface and contact angle values of the surfactants, with and without glyphosate, showed a significant increase as the PEO number increased in both surfactant series. A higher absorption of the 14C-glyphosate was recorded for S. spinosa compared with S. obtusifolia. The absorption and translocation of the 14C-glyphosate increased with the increase in the PEO number of tallow amine ethoxylate. In the case of the non-ionic alkoxylate surfactant series, an increase in the absorption of 14C-glyphosate was recorded when the surface tension and contact angle values decreased. There was no significant difference in the translocation values obtained in the two species after the addition of the surfactants. The amount of 14C-glyphosate absorbed by the treated leaf was significantly higher in the case of S. spinosa compared with S. obtusifolia. A linear relationship was observed with the physical properties, 14C absorption, and the efficacy of glyphosate with the addition of the non-ionic alkoxylate surfactant series. The percentage control was higher with the higher PEO surfactant in the tallow amine ethoxylate surfactant series and with the lower PEO surfactant in the non-ionic alkoxylate surfactant series as the two series are chemically different.  相似文献   

19.
Chlorsulfuron, giyphosate and imazaquin were evaluated in pot and field studies for their efficacy in controlling broomrape (Orobanche ramosa L.) in tomato (Lycopersicon esculentum Mill.) in Northern Greece. All herbicides were applied four to five weeks after tomato transplanting, when the crop was at early flowering stage and broomrape had started to develop underground attachments. The number of emerged broomrape shoots and underground attachments were less affected by herbicide treatments than the dry weight, suggesting that the herbicides suppress the growth of broomrape rather than kill its underground organs. In the pot experiments, chlorsulfuron applied at 5 g AI ha?1 was the most effective treatment for broomrape control and the least toxic to the crop. Imazaquin and glyphosate applied at 37 and 180 g AI ha?1, respectively, controlled broomrape but imazaquin reduced crop yield. In the field, similar rates of glyphosate and higher rates of imazaquin were not toxic to the crop but were less effective on broomrape. Chlorsulfuron applied at 10 g AI ha?1 controlled broomrape emergence by 88%. When the herbicide was applied twice (5+10 g AI ha?1), it gave complete control of broomrape but delayed crop maturity. The yield of tomato was not increased as a result of these treatments because of low broomrape infestation and a short competition period.  相似文献   

20.
The effect of glyphosate on weeds was evaluated in greenhouse bioassays with five weed species and compared with a commercial mixture of phenmedipham and ethofumesate. Glyphosate was more active than a mixture of phenmedipham and ethofumesate on the weeds. Solanum nigrum was the most sensitive species to both herbicide treatments. The relative potency of glyphosate between the weeds showed consistency at 50% and 90% control levels for four out of five weed species, which could be ranked independently of control level because of similar response curves. In a field trial on sugarbeet genetically engineered to acquire glyphosate tolerance, a total of 720 g a.i. ha?1 of glyphosate applied in one, two or three applications gave similar or superior control of weeds to a total of 3.17 kg a.i. ha?1 mixture of metamitron, phenmedipham and ethofumesate applied in three repeated applications.  相似文献   

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