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以蛋白桑的幼嫩叶片为外植体,以MS为基本培养基,采用正交实验设计,研究不同浓度的植物生长调节剂对蛋白桑植株再生的影响。结果表明:叶片诱导愈伤组织的最佳培养基为MS+TDZ 1.0mg·L~(-1)+NAA 0.1mg·L~(-1);最佳分化培养基为MS+TDZ 0.2mg·L~(-1)+NAA 0.09mg·L~(-1),分化率为21.65%;培养基1/2MS+PVP 0.2g·L~(-1)+NAA 0.5mg·L~(-1)生根率最高,达到66.70%;对草丁膦的敏感性试验中,草丁膦浓度0.04mg·L~(-1)为蛋白桑叶片半致死浓度,草丁膦浓度0.05mg·L~(-1)对蛋白桑叶片的致死率为75.00%,草丁膦浓度0.07mg·L~(-1)的致死率为100.00%。 相似文献
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以木榄幼叶为外植体,进行其愈伤组织诱导的影响因子研究,通过灭菌条件、培养基成分、添加剂种类、外源植物激素浓度试验,比较外植体褐化率与愈伤组织诱导率。结果表明:75%乙醇灭菌30s后在2%(体积分数v/v)NaClO溶液灭菌20min是木榄幼叶表面消毒适宜条件;氨基酸培养基是基础培养基;45℃热激与添加1.0mmol·L~(-1)抗坏血酸均可降低褐化率;AA培养基+0.50mg·L~(-1) 2,4-D+0.50mg·L~(-1) TDZ+1.00mmol·L~(~(-1))维生素C或AA培养基+0.50mg·L~(-1) 2,4-D+0.50mg·L~(-1) CPPU+1.00mmol·L~(-1)维生素C均可得大于50%的愈伤诱导率。因此,克服外植体褐化与应用CPPU或TDZ作为细胞分裂素是木榄愈伤组织诱导成功的关键。 相似文献
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以3种食用百合试管苗为试材,采用限制生长保存法,研究了不同浓度生长抑制剂对食用百合试管苗保存效果的影响,并对保存后的试管苗进行遗传稳定性检测,以期建立食用百合种质资源离体保存体系。结果表明:龙芽百合试管苗用10mg·L~(-1)防落素(PCPA)处理保存300d后,试管苗生长缓慢,存活率达96.00%;川百合试管苗用2.0mg·L~(-1)青鲜素(MH)处理保存150d后,试管苗的抑制生长效果明显,结鳞率和存活率高达100.00%;兰州百合试管苗用10mg·L~(-1)多效唑(PP333)处理保存300d后,试管苗抑制作用明显,结鳞率达100.00%,存活率达94.40%。比较限制生长保存后与未保存植株的可溶性蛋白和酯酶同工酶图谱,各处理和对照图谱带相似,初步证明了以上保存方法的可行性,较好的保持了遗传稳定性。 相似文献
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以燕子掌叶片为试材,采用植物组织培养方法,研究了消毒时间、不同浓度2,4-D、6-BA、NAA对燕子掌再生的影响。结果表明:叶片用70%~75%的酒精浸泡10~15s,再用0.1%HgCl2消毒12~15min,具有较低污染率和较高的存活率;用MS+1.5mg·L~(-1) 6-BA+0.5mg·L~(-1)2,4-D进行不定芽诱导,平均可以产生13.7个不定芽;不定芽在MS+6-BA 1.5mg·L~(-1)+NAA0.1mg·L~(-1)+GA30.5mg·L~(-1)中增殖倍数为11.0;在1/2MS+IBA 0.5mg·L~(-1)或MS+IBA0.5mg·L~(-1)培养基中平均能产生9~11条根。 相似文献
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以"灵武长枣"为试材,采用完全随机区组设计,设置增氧浓度(5±0.5)(TR1)、(7±0.5)(TR2)、(9±0.5)(TR3)、(3±0.5)(CK)mg·L~(-1)4个处理,分析了不同增氧灌溉处理对"灵武长枣"生长与果实品质的影响,以确定适宜的增氧浓度。结果表明:(7±0.5)mg·L~(-1)(TR2)处理下,枣吊长增长量较大、叶绿素含量较高。(9±0.5)mg·L~(-1)(TR3)处理下,对枣吊增粗作用效果最显著。(5±0.5)(TR1)、(7±0.5)(TR2)mg·L~(-1)处理对提高单果质量、维生素C、可溶性总糖等指标方面作用显著;综上所述,增氧浓度为(7±0.5)mg·L~(-1)的处理能促进"灵武长枣"生长,提高果实品质,可在生产中推广使用。 相似文献
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目的：探讨姬松茸多糖（Agaricus blazeiMuril lPolysac．charides,ABPS）对体外高糖培养乳鼠心肌细胞凋亡的抑制作用及其机制。方法：将Wistar乳鼠原代细胞随机分为5组,A：空白对照组;B：高糖组;C：0．4mg／mLABPS组;D：0．8mg／mLABPS组。用荧光显微镜测定各组细胞凋亡情况,RT—PCR法检测bcl-2、caspase-3基因表达量的变化。结果：经ABPS干预后,凋亡细胞明显减少,bcl-2表达增加,caspase～3表达减少。结论：ABPS抑制体外高糖培养的乳鼠心肌的凋亡,其机制可能与抑制caspase-3、激活bcl-2表达有关。 相似文献
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《中国瓜菜》2021,(7)
为探索缓慢生长法离体保存技术在韭菜中的应用,以航研998为试验材料,利用植物组织培养法,在MS+6-BA 0.5 mg·L~(-1)+NAA 0.1 mg·L~(-1)的基本培养基中添加不同浓度的山梨醇、琼脂、蔗糖、矮壮素(CCC)和脱落酸(ABA)对韭菜组培苗进行保存,每隔20 d调查1次组培苗的不定芽数、株高和存活率。结果表明,20 g·L~(-1)的山梨醇、70 g·L~(-1)的蔗糖、250 mg·L~(-1)的CCC和1.0 mg·L~(-1)的ABA均能有效抑制韭菜组培苗的生长,且在保存120 d后保持较高的成活率和良好的生长表现。研究结果为韭菜缓慢生长法离体保存体系的建立提供了理论依据。 相似文献
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《北方园艺》2020,(2)
以"黄梦脆"厚皮甜瓜为试材,采用不增氧灌溉(CK,溶解氧浓度为3.4 mg·L~(-1))和4个增氧灌溉处理(T1:微纳米气泡发生器增氧灌溉,溶解氧浓度6.8 mg·L~(-1);T2、T3处理:过氧化尿素增氧灌溉,溶解氧浓度分别为6.8、10.2 mg·L~(-1);T4处理:坐果前过氧化尿素增氧灌溉,溶解氧浓度为10.2 mg·L~(-1);坐果后微纳米气泡发生器增氧灌溉,溶解氧浓度为6.8 mg·L~(-1)),通过对甜瓜各时期植株的生长性状和品质、产量指标进行测量,研究不同增氧灌溉方式对甜瓜植株生长和果实的品质、产量的影响,为甜瓜增氧灌溉提供参考依据。结果表明:2种增氧方式效果明显;采用过氧化尿素增氧灌溉时(T2、T3、T4处理),对甜瓜的产量和品质都造成不利影响;当全生育期采用微纳米气泡发生器增氧灌溉(T1处理)时,植株各生长时期的株高、主蔓粗、平均叶长、平均叶宽等性状均大于其它处理和对照,果实的平均单果质量和果肉厚度、可溶性固形物含量、产量、品质等性状表现为最优。大棚甜瓜种植采用微纳米气泡发生器加氧灌溉,促进植株生长,增加甜瓜的产量,安装简单、使用成本低,是一项值得推广的农用装备。 相似文献
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为评价茯苓(Poria cocos)多糖对非小细胞肺癌NCI-H460细胞的抑制作用,用CCK-8法测定茯苓多糖对细胞增殖的抑制率,用DAPI染色法、AOEB双荧光染色法分析茯苓多糖对细胞凋亡的影响,用细胞划伤愈合实验测定茯苓多糖对细胞迁移能力的影响,用蛋白免疫印迹实验检测茯苓多糖对细胞凋亡、迁移相关蛋白表达的影响。结果表明：茯苓多糖具有抑制NCI-H460细胞增殖、集落形成和迁移的能力,可诱导NCI-H460细胞凋亡;500、1 000μg·mL-1的茯苓多糖可使Bax和P53蛋白表达量升高,Bcl-2和MMP-2蛋白表达量降低。 相似文献
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AIM: To investigate the apoptosis of implanted tumor of primary human gastric cancer cells in nude mice induced by genistein and the relation between this apoptosis and expression of bcl-2 and bax.METHODS: Establishing a transplanted tumor model by injecting human primary gastric cancer cells into subcutaneous tissue of nude mice.The different doses of genistein (0.5mg/kg,1mg/kg and 1.5 mg/kg ) were directly injected beside tumor body respectively,for six times at an interval of two days.Then changes of tumor volume were measured continuously and tumor inhibition rate of each group was calculated.We observed the morphologic alteration by electron microscope,measured the apoptotic rate by TUNEL staining method,detected the expression of apoptosis-regulated gene bcl-2 and bax by immunohistochemical staining and RT-PCR.RESULTS: Genistein could significantly inhibit carcinoma growth when it was injected near the carcinoma.Genistein induced implanted tumors cells to undergo apoptosis with apoptotic characteristics by transmission electron microscope.The apoptosis index of above three groups was increased progressively.Positive rate of Bcl-2 protein of above three groups was decreased progressively and positive rate of Bax protein of above three groups was increased progressively by immunohistochemical staining.The density of bcl-2 mRNA decreased progressively and the density of bax mRNA increased progressively with elongation of time by RT-PCR.CONCLUSION: Genistein is able to induce the apoptosis of transplanted tumor cells.This apoptosis may be mediated by down-regulating bcl-2 and up-regulating bax mRNA and its protein. 相似文献
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AIM: To investigate the effects and related mechanism of fucoidan on the proliferation and apoptosis in breast carcinoma cell line MCF-7. METHODS: MCF-7 cells were treated with different concentrations of fucoidan (100 mg/L, 300 mg/L, 500 mg/L, 1 000 mg/L) for 48 h. Cell viability was measured by MTT assay. Apoptosis morphological and biochemical changes were detected by Hoechst 33258 staining and agarose gel electrophoresis. The expression of 〖STBX〗bcl-2〖STBZ〗 and bax was examined by RT-PCR and Western blotting. RESULTS: Fucoidan at different concentrations (100 mg/L, 300 mg/L, 500 mg/L, 1 000 mg/L) effectively inhibited the proliferation of MCF-7 cells (P<0.01). The inhibitory ratio and apoptosis rate increased in a concentration-dependent manner. Agarose gel electrophoresis of DNA revealed the characteristic “ladder” pattern of apoptosis. Fucoidan down-regulated the expression of 〖STBX〗bcl-2〖STBZ〗 and up-regulated bax in the levels of mRNA and protein. The ratio of Bcl-2 to Bax decreased as the concentrations of fucoidan increased (P<0.05). CONCLUSION: Fucoidan inhibits the cell proliferation by inducing cell apoptosis, and the apoptosis is related to the down-regulation of anti-apoptotic protein Bcl-2 and up-regulation of apoptotic protein Bax. 相似文献
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AIM：To explore the effects of tetrahydroxystilbene-2-O-β-D-glucoside (TSG) from Polygonum multiflorum on the apoptosis and the mRNA expression of bcl-2, bax and caspase-3 in human umbilical vein endothelial cells (HUVECs) treated with homocysteine (Hcy). METHODS：Cultured HUVECs were treated with Hcy (3 mmol/L) to establish a Hcy-damaged model. HUVECs in TSG treated groups were pre-incubated with TSG at concentrations of 1 μmol/L and 10 μmol/L for 2 h before treated with Hcy. Cell nuclear damage was detected by Hoechst 33342 staining. Cell apoptosis was determined by flow cytometry. The mRNA expression of bcl-2, bax and caspase-3 was measured by real-time fluorescence quantitative RT-PCR. RESULTS： After treatment with Hcy at concentration of 3 mmol/L, the nuclear damage and apoptotic rate of HUVECs were higher than that in normal group. The expression of bcl-2 was lower, and the expression of Bax and caspase-3 was higher than that in normal group. On the other hand, pre-incubation with TSG at concentrations of 1 μmol/L and 10 μmol/L decreased the nuclear damage and cell apoptosis, increased the expression of bcl-2, and decreased the expression of bax and caspase-3 as compared with the cells only treated with Hcy. CONCLUSION：TSG reduces the apoptosis of HUVECs induced by Hcy, and the mechanism might be associated with regulating the expression of bcl-2, bax and caspase-3. 相似文献
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XIE Kai-qing YANG Hai-bo LIN Hong-sheng CHEN Li HUO Dong-mei SHI Ying-long QIN Shi-yan ZHOU Hong-wei 《园艺学报》2016,32(11):2020-2025
ATM: To explore whether the C-reactive protein (CRP) level in microinflammation state induces the apoptosis of renal tubular epithelial cells. METHODS: HK-2 cells were stimulated with recombinant human CRP. Annexin-FITC-PI staining and flow cytometry were used to detect the percentage of apoptotic cells. Morphology observation of apoptosis was assessed by Hoechst 33258 staining. Caspase-3 activity was measured by a colorimetric assay. The expression of apoptotic gene bax and anti-apoptotic gene bcl-2 at mRNA levels was determined by real-time PCR. RESULTS: CRP induced apoptosis of HK-2 cells in a time- and dose-dependent manner. The maximal apoptotic effect of CRP concentration was 10 mg/L CRP at concentration of 20 mg/L. CRP treatment was associated with the characteristic morphological features of apoptosis such as condensation, fragmentation or margination of nuclear chromatin. CRP exposure increased caspase-3 activity, up-regulated the mRNA expression of Bax and down-regulated the mRNA expression of Bcl-2. CONCLUSION: Slightly increased CRP level has the potential to induce apoptosis of renal tubular cells. 相似文献
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AIM: To investigate the apoptosis in primary gastric cancer cells induced by resveratrol, and the relation between this apoptosis and expression of bcl-2 and bax. METHODS: In in vitro experiments, MTT assay was used to determine the cell growth inhibitory rate. Transmission electron microscopy and TUNEL staining were used to quantitatively and qualitively detect the apoptosis of primary gastric cancer cells before and after the resveratrol treatment. Immunohistochemical staining and RT-PCR was used to detect the expression of apoptosis-regulated gene bcl-2 and bax. RESULTS: Resveratrol inhibited the growth of primary gastric cancer cells in a dose- and time-dependent manner. Resveratrol induced primary gastric cancer cells to undergo apoptosis with typically apoptotic characteristics. TUNEL assay showed that after the treatment of primary gastric cancer cells with resveratrol for 24, 48, 72, 96 hours, the apoptotic indexs were 4.93%±0.19%, 16.74%±0.43%, 27.88%±0.36%, 36.84%±1.07% respectively. Immunohistochemical staining showed that after the treatment of primary gastric cancer cells with resveratrol for 24, 48, 72, 96 hours, the positive rates of Bcl-2 proteins were 20.68%±0.49%, 10.84%±0.33%, 6.80%±0.34%, 3.91%±0.15% and the positive rates of Bax proteins were 19.79%±0.98%, 30.74%±0.85%, 40.14%±1.17%, 60.08%±1.64%. After exposed to resveratrol for 24 h, 48 h, 72 h and 96 h, the density of bcl-2 mRNA decreased progressively with elongation of time and the density of bax mRNA increased progressively with elongation of time by RT-PCR. CONCLUSION: Resveratrol is able to induce the apoptosis in primary gastric cancer. This apoptosis may be mediated by down-regulation of Bcl-2 and up-regulation of Bax. 相似文献
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AIM: To investigate the molecular mechanism of the apoptosis of implanted tumor of human primary gastric cancer cells in nude mice induced by resveratrol. METHODS: Human primary gastric cancer cells were planted into nude mice to establish the cancer model. Resveratrol at different doses were injected near the carcinoma on the nude mice. After treatment, transmission electron microscope and TUNEL staining method were used to detect the apoptosis of implanted tumor cells. Immunohistochemical staining and RT-PCR were used to detect the expression of apoptosis-related genes bcl-2 and bax in implanted tumor. RESULTS: Resveratrol significantly inhibited carcinoma growth when it was injected near the carcinoma. The apoptotic cells in implanted tumor induced by resveratrol were detected by transmission electron microscope and TUNEL staining, immunohistochemical staining and RT-PCR showed resveratrol inhibited bcl-2 expression and increased bax expression in human primary gastric cancer cells. CONCLUSION: Resveratrol inhibits implanted tumor of human primary gastric cancer cells in nude mice through inducing apoptosis. This apoptosis may be mediated by down-regulation of bcl-2 expression and up-regulation of bax expression. 相似文献
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GUO Jin XU Chang-qing LI Hong-zhu PEI Tian-xian WANG Li-na ZHANG Li ZHANG Wei-hua XU Man LIN Yan TIAN Ye 《园艺学报》2009,25(1):48-53
AIM:To observe the change of calcium sensing receptor (CaSR) expression and apoptotic pathways in myocardial infarction rat induced by isoprel.METHODS: The myocardial infarction rat models were prepared by subcutaneous injection of isoprel (ISO 200 mg·kg-1·d-1 for 2 d). Wistar rats were divided into three groups randomly: ① Control group; ② ISO/1d group; ③ ISO /2d group. The expressions of CaSR, 〖STBX〗bcl-2, bax〖STBZ〗 and caspase-3 mRNA and protein were analyzed by RT-PCR and Western blotting, respectively. Apoptotic cells were measured by TUNEL staining assay. The morphological and ultrastructural changes were observed under optical microscope and electronic microscope. The activity of LDH, CK, SOD and the content of MDA were assayed with ultraviolet spectrophotometer. The level of troponin(cTnT) was observed by chemical immunofluoresence.RESULTS: Compared with control group, the activity of LDH and CK, the content of MDA and cTnT, the apoptosis index and the expression of CaSR, Bax and caspase-3 were reached the highest level, but the SOD activity and Bcl-2 expression were decreased. The myocardial ultrastructural injury was aggravated in the ISO/1d group. The change of above parameters in ISO/2d group was between control and ISO/1d group.CONCLUSION:The increased expression of CaSR is involved in rat myocardial infarction induced by isoprel, which is related with oxidative stress and apoptosis. 相似文献
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AIM: To observe the effects of epigallocatechin gallate (EGCG) on cardiomyocyte apoptosis induced by ischemia-reperfusion (IR) in rats. METHODS: The left anterior descending branch of coronary artery was ligated for 30 min and reperfused for 60 min to make a the myocardial ischemia-reperfusion model in rats. The experiment was divided into five groups: sham, ischemia/reperfusion (IR), EGCG (10 mg/kg and 20 mg/kg) and salvia miltiorrhizae (SM, 100 mg/kg) group. The apoptotic cardiomyocytes were detected by in situ end labeling method, and the expressions of Bcl-2 and Bax were shown through immunohistochemistry method. RESULTS: There was no apoptosis myocardial cell in sham operation group. The apoptosis index and expression of bax significantly increased, and bcl-2/bax reduced in IR group (P<0.01). In EGCG-treated group, however, the changes above were obviously alleviated (P<0.01). CONCLUSION: EGCG significantly inhibits cardiomyocyte apoptosis in ischemia-reperfusion rat hearts. The possible mechanism is to raise the ratio of Bcl-2/Bax proteins by increasing in the expression of bcl-2 gene and decreasing in the expression of bax gene. 相似文献
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AIM: This study aimed to observe the effects of high-glucose on proliferation and apoptosis of endothelial progenitor cells (EPCs) in type 2 diabetes mellitus patients,and tried to elucidate their possible role.METHODS: Various concentrations of glucose were added to the culture system of EPCs from 25 cases of type 2 diabetes mellitus patients (DM group) and 25 cases of healthy volunteers (control group).MTT assays were used to detect the proliferative rates.Annexin-V/PI stains were used to detect the apoptotic rates,and RT-PCR to detect the expression level of bcl-2 and bax.RESULTS: Proliferative activity of EPCs in both control group and DM group were attenuated when concentration of glucose was 33 mmol/L,while apoptotic rates increased.No significant change of proliferative rate and apoptotic rate of EPCs in DM group and control group in the presence of 5 mmol/L glucose was observed.The expression level of bax of EPCs in both DM group and control group increased while expression level of bcl-2 did not change much in the presence of 33 mmol/L glucose.CONCLUSION: High-glucose attenuates proliferative activity of EPCs and increases the apoptotic rate.Upregulation of bax may be its possible role. 相似文献