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1.
Saprobic Cladosporium isolates morphologically similar to C. sphaerospermum are phylogenetically analysed on the basis of DNA sequences of the ribosomal RNA gene cluster, including the internal transcribed spacer regions ITS1 and ITS2, the 5.8S rDNA (ITS) and the small subunit (SSU) rDNA as well as β-tubulin and actin gene introns and exons. Most of the C. sphaerospermum-like species show halotolerance as a recurrent feature. Cladosporium sphaerospermum, which is characterised by almost globose conidia, is redefined on the basis of its ex-neotype culture. Cladosporium dominicanum, C. psychrotolerans, C. velox, C. spinulosum and C. halotolerans, all with globoid conidia, are newly described on the basis of phylogenetic analyses and cryptic morphological and physiological characters. Cladosporium halotolerans was isolated from hypersaline water and bathrooms and detected once on dolphin skin. Cladosporium dominicanum and C. velox were isolated from plant material and hypersaline water. Cladosporium psychrotolerans, which grows well at 4 °C but not at 30 °C, and C. spinulosum, having conspicuously ornamented conidia with long digitate projections, are currently only known from hypersaline water. We also newly describe C. salinae from hypersaline water and C. fusiforme from hypersaline water and animal feed. Both species have ovoid to ellipsoid conidia and are therefore reminiscent of C. herbarum. Cladosporium langeronii (= Hormodendrum langeronii) previously described as a pathogen on human skin, is halotolerant but has not yet been recorded from hypersaline environments.Taxonomic novelties: Cladosporium dominicanum Zalar, de Hoog & Gunde-Cimerman, sp. nov., C. fusiforme Zalar, de Hoog& Gunde-Cimerman, sp. nov., C. halotolerans Zalar, de Hoog & Gunde-Cimerman, sp. nov., C. psychrotolerans Zalar, de Hoog & Gunde-Cimerman, sp. nov., C. salinae Zalar, de Hoog & Gunde-Cimerman, sp. nov., C. spinulosum Zalar, de Hoog & Gunde-Cimerman, sp. nov., C. velox Zalar, de Hoog & Gunde-Cimerman, sp. nov.  相似文献   

2.
Although morphologically similar, species of Cladophialophora (Herpotrichiellaceae) were shown to be phylogenetically distinct from Pseudocladosporium (Venturiaceae), which was revealed to be synonymous with the older genus, Fusicladium. Other than being associated with human disorders, species of Cladophialophora were found to also be phytopathogenic, or to occur as saprobes on organic material, or in water, fruit juices, or sports drinks, along with species of Exophiala. Caproventuria and Metacoleroa were confirmed to be synonyms of Venturia, which has Fusicladium (= Pseudocladosporium) anamorphs. Apiosporina, based on A. collinsii, clustered basal to the Venturia clade, and appears to represent a further synonym. Several species with a pseudocladosporium-like morphology in vitro represent a sister clade to the Venturia clade, and are unrelated to Polyscytalum. These taxa are newly described in Fusicladium, which is morphologically close to Anungitea, a heterogeneous genus with unknown phylogenetic affinity. In contrast to the Herpotrichiellaceae, which were shown to produce numerous synanamorphs in culture, species of the Venturiaceae were morphologically and phylogenetically more uniform. Several new species and new combinations were introduced in Cladophialophora, Cyphellophora (Herpotrichiellaceae), Exophiala, Fusicladium, Venturia (Venturiaceae), and Cylindrosympodium (incertae sedis).Taxonomic novelties: Cladophialophora australiensis Crous& A.D. Hocking, sp. nov., Cladophialophora chaetospira (Grove) Crous & Arzanlou, comb. nov., Cladophialophora hostae Crous, U. Braun & H.D. Shin, sp. nov., Cladophialophora humicola Crous& U. Braun, sp. nov., Cladophialophora potulentorum Crous & A.D. Hocking, sp. nov., Cladophialophora scillae (Deighton) Crous, U. Braun & K. Schub., comb. nov., Cladophialophora sylvestris Crous& de Hoog, sp. nov., Cylindrosympodium lauri Crous & R.F. Castañeda, sp. nov., Cyphellophora hylomeconis Crous, de Hoog& H.D. Shin, sp. nov., Exophiala eucalyptorum Crous, sp. nov., Fusicladium africanum Crous, sp. nov., Fusicladium amoenum (R.F. Castañeda & Dugan) Crous, K. Schub. & U. Braun, comb. nov., Fusicladium brevicatenatum (U. Braun & Feiler) Crous, U. Braun & K. Schub., comb. nov., Fusicladium fagi Crous & de Hoog, sp. nov., Fusicladium intermedium (Crous & W.B. Kendr.) Crous, comb. nov., Fusicladium matsushimae (U. Braun & C.F. Hill) Crous, U. Braun & K. Schub., comb. nov., Fusicladium pini Crous& de Hoog, sp. nov., Fusicladium ramoconidii Crous & de Hoog, sp. nov., Fusicladium rhodense Crous & M.J. Wingf., sp. nov., Venturia hystrioides (Dugan, R.G. Roberts & Hanlin) Crous & U. Braun, comb. nov.  相似文献   

3.
The phylogeny of the genera Periconiella, Ramichloridium, Rhinocladiella and Veronaea was explored by means of partial sequences of the 28S (LSU) rRNA gene and the ITS region (ITS1, 5.8S rDNA and ITS2). Based on the LSU sequence data, ramichloridium-like species segregate into eight distinct clusters. These include the Capnodiales (Mycosphaerellaceae and Teratosphaeriaceae), the Chaetothyriales (Herpotrichiellaceae), the Pleosporales, and five ascomycete clades with uncertain affinities. The type species of Ramichloridium, R. apiculatum, together with R. musae, R. biverticillatum, R. cerophilum, R. verrucosum, R. pini, and three new species isolated from Strelitzia, Musa and forest soil, respectively, reside in the Capnodiales clade. The human-pathogenic species R. mackenziei and R. basitonum, together with R. fasciculatum and R. anceps, cluster with Rhinocladiella (type species: Rh. atrovirens, Herpotrichiellaceae, Chaetothyriales), and are allocated to this genus. Veronaea botryosa, the type species of the genus Veronaea, also resides in the Chaetothyriales clade, whereas Veronaea simplex clusters as a sister taxon to the Venturiaceae (Pleosporales), and is placed in a new genus, Veronaeopsis. Ramichloridium obovoideum clusters with Carpoligna pleurothecii (anamorph: Pleurothecium sp., Chaetosphaeriales), and a new combination is proposed in Pleurothecium. Other ramichloridium-like clades include R. subulatum and R. epichloës (incertae sedis, Sordariomycetes), for which a new genus, Radulidium is erected. Ramichloridium schulzeri and its varieties are placed in a new genus, Myrmecridium (incertae sedis, Sordariomycetes). The genus Pseudovirgaria (incertae sedis) is introduced to accommodate ramichloridium-like isolates occurring on various species of rust fungi. A veronaea-like isolate from Bertia moriformis with phylogenetic affinity to the Annulatascaceae (Sordariomycetidae) is placed in a new genus, Rhodoveronaea. Besides Ramichloridium, Periconiella is also polyphyletic. Thysanorea is introduced to accommodate Periconiella papuana (Herpotrichiellaceae), which is unrelated to the type species, P. velutina (Mycosphaerellaceae).Taxonomic novelties: Myrmecridium Arzanlou, W. Gams & Crous, gen. nov., Myrmecridium flexuosum (de Hoog) Arzanlou, W. Gams& Crous, comb. et stat. nov., Myrmecridium schulzeri (Sacc.) Arzanlou, W. Gams & Crous var. schulzeri, comb. nov., Myrmecridium schulzeri var. tritici (M.B. Ellis) Arzanlou, W. Gams & Crous, comb. nov., Periconiella arcuata Arzanlou, S. Lee & Crous, sp. nov., Periconiella levispora Arzanlou, W. Gams& Crous, sp. nov., Pleurothecium obovoideum (Matsush.) Arzanlou& Crous, comb. nov., Pseudovirgaria H.D. Shin, U. Braun, Arzanlou& Crous, gen. nov., Pseudovirgaria hyperparasitica H.D. Shin, U. Braun, Arzanlou & Crous, sp. nov., Radulidium Arzanlou, W. Gams& Crous, gen. nov., Radulidium epichloës (Ellis & Dearn.) Arzanlou, W. Gams & Crous, comb. nov., Radulidium subulatum (de Hoog) Arzanlou, W. Gams & Crous, comb. nov., Ramichloridium australiense Arzanlou & Crous, sp. nov., Ramichloridium biverticillatum Arzanlou & Crous, nom. nov., Ramichloridium brasilianum Arzanlou & Crous, sp. nov., Ramichloridium strelitziae Arzanlou, W. Gams & Crous, sp. nov., Rhinocladiella basitona (de Hoog) Arzanlou & Crous, comb. nov., Rhinocladiella fasciculata (V. Rao & de Hoog) Arzanlou & Crous, comb. nov., Rhinocladiella mackenziei (C.K. Campb. & Al-Hedaithy) Arzanlou & Crous, comb. nov., Rhodoveronaea Arzanlou, W. Gams & Crous, gen. nov., Rhodoveronaea varioseptata Arzanlou, W. Gams & Crous, sp. nov., Thysanorea Arzanlou, W. Gams& Crous, gen. nov., Thysanorea papuana (Aptroot) Arzanlou, W. Gams & Crous, comb. nov., Veronaea japonica Arzanlou, W. Gams& Crous, sp. nov., Veronaeopsis Arzanlou & Crous, gen. nov., Veronaeopsis simplex (Papendorf) Arzanlou & Crous, comb.nov.  相似文献   

4.
Cladophialophora is a genus of black yeast-like fungi comprising a number of clinically highly significant species in addition to environmental taxa. The genus has previously been characterized by branched chains of ellipsoidal to fusiform conidia. However, this character was shown to have evolved several times independently in the order Chaetothyriales. On the basis of a multigene phylogeny (nucLSU, nucSSU, RPB1), most of the species of Cladophialophora (including its generic type C. carrionii) belong to a monophyletic group comprising two main clades (carrionii- and bantiana-clades). The genus includes species causing chromoblastomycosis and other skin infections, as well as disseminated and cerebral infections, often in immunocompetent individuals. In the present study, multilocus phylogenetic analyses were combined to a morphological study to characterize phenetically similar Cladophialophora strains. Sequences of the ITS region, partial Translation Elongation Factor 1-α and β-Tubulin genes were analysed for a set of 48 strains. Four novel species were discovered, originating from soft drinks, alkylbenzene-polluted soil, and infected patients. Membership of the both carrionii and bantiana clades might be indicative of potential virulence to humans.Taxonomic novelties: Cladophialophora samoënsis Badali, de Hoog & Padhye, sp. nov., Cladophialophora subtilis Badali & de Hoog, sp. nov., Cladophialophora mycetomatis Badali, de Hoog & Bonifaz, sp. nov., Cladophialophora immunda Badali, Satow, Prenafeta-Boldú, Padhye & de Hoog, sp. nov.  相似文献   

5.
The Capnodiales incorporates plant and human pathogens, endophytes, saprobes and epiphytes, with a wide range of nutritional modes. Several species are lichenised, or occur as parasites on fungi, or animals. The aim of the present study was to use DNA sequence data of the nuclear ribosomal small and large subunit RNA genes to test the monophyly of the Capnodiales, and resolve families within the order. We designed primers to allow the amplification and sequencing of almost the complete nuclear ribosomal small and large subunit RNA genes. Other than the Capnodiaceae (sooty moulds), and the Davidiellaceae, which contains saprobes and plant pathogens, the order presently incorporates families of major plant pathological importance such as the Mycosphaerellaceae, Teratosphaeriaceae and Schizothyriaceae. The Piedraiaceae was not supported, but resolves in the Teratosphaeriaceae. The Dissoconiaceae is introduced as a new family to accommodate Dissoconium and Ramichloridium. Lichenisation, as well as the ability to be saprobic or plant pathogenic evolved more than once in several families, though the taxa in the upper clades of the tree lead us to conclude that the strictly plant pathogenic, nectrotrophic families evolved from saprobic ancestors (Capnodiaceae), which is the more primitive state.Taxonomic novelties: Brunneosphaerella Crous, gen. nov., B. jonkershoekensis (Marinc., M.J. Wingf. & Crous) Crous, comb. nov., B. protearum (Syd. & P. Syd.) Crous, comb. nov., Devriesia hilliana Crous & U. Braun, sp. nov., D. lagerstroemiae Crous & M.J. Wingf., sp. nov., D. strelitziicola Arzanlou & Crous, sp. nov., Dissoconiaceae Crous & de Hoog, fam. nov., Hortaea thailandica Crous & K.D. Hyde, sp. nov., Passalora ageratinae Crous & A.R. Wood, sp. nov., P. armatae Crous & A.R. Wood, sp. nov., Rachicladosporium cboliae Crous, sp. nov.  相似文献   

6.
7.
The genus Cladosporium is restricted to dematiaceous hyphomycetes with a coronate scar type, and Davidiella teleomorphs. In the present study numerous cladosporium-like taxa are treated, and allocated to different genera based on their morphology and DNA phylogeny derived from the LSU nrRNA gene. Several species are introduced in new genera such as Hyalodendriella, Ochrocladosporium, Rachicladosporium, Rhizocladosporium, Toxicocladosporium and Verrucocladosporium. A further new taxon is described in Devriesia (Teratosphaeriaceae). Furthermore, Cladosporium castellanii, the etiological agent of tinea nigra in humans, is confirmed as synonym of Stenella araguata, while the type species of Stenella is shown to be linked to the Teratosphaeriaceae (Capnodiales), and not the Mycosphaerellaceae as formerly presumed.Taxonomic novelties: Devriesia americana Crous & Dugan, sp. nov., Hyalodendriella Crous, gen. nov., Hyalodendriella betulae Crous sp. nov., Ochrocladosporium Crous & U. Braun, gen. nov., Ochrocladosporium elatum (Harz) Crous & U. Braun, comb. nov., Ochrocladosporium frigidarii Crous & U. Braun, sp. nov., Rachicladosporium Crous, U. Braun & Hill, gen. nov., Rachicladosporium luculiae Crous, U. Braun & Hill, sp. nov., Rhizocladosporium Crous & U. Braun, gen. nov., Rhizocladosporium argillaceum (Minoura) Crous & U. Braun, comb. nov., Toxicocladosporium Crous & U. Braun, gen. nov., Toxicocladosporium irritans Crous & U. Braun, sp. nov., Verrucocladosporium K. Schub., Aptroot & Crous, gen. nov., Verrucocladosporium dirinae K. Schub., Aptroot & Crous, sp. nov.  相似文献   

8.
The genus Quambalaria consists of plant-pathogenic fungi causing disease on leaves and shoots of species of Eucalyptus and its close relative, Corymbia. The phylogenetic relationship of Quambalaria spp., previously classified in genera such as Sporothrix and Ramularia, has never been addressed. It has, however, been suggested that they belong to the basidiomycete orders Exobasidiales or Ustilaginales. The aim of this study was thus to consider the ordinal relationships of Q. eucalypti and Q. pitereka using ribosomal LSU sequences. Sequence data from the ITS nrDNA were used to determine the phylogenetic relationship of the two Quambalaria species together with Fugomyces (= Cerinosterus) cyanescens. In addition to sequence data, the ultrastructure of the septal pores of the species in question was compared. From the LSU sequence data it was concluded that Quambalaria spp. and F. cyanescens form a monophyletic clade in the Microstromatales, an order of the Ustilaginomycetes. Sequences from the ITS region confirmed that Q. pitereka and Q. eucalypti are distinct species. The ex-type isolate of F. cyanescens, together with another isolate from Eucalyptus in Australia, constitute a third species of Quambalaria, Q. cyanescens (de Hoog & G.A. de Vries) Z.W. de Beer, Begerow & R. Bauer comb. nov. Transmission electron-microscopic studies of the septal pores confirm that all three Quambalaria spp. have dolipores with swollen lips, which differ from other members of the Microstromatales (i.e. the Microstromataceae and Volvocisporiaceae) that have simple pores with more or less rounded pore lips. Based on their unique ultrastructural features and the monophyly of the three Quambalaria spp. in the Microstromatales, a new family, Quambalariaceae Z.W. de Beer, Begerow & R. Bauer fam. nov., is described.Taxonomic novelties: Quambalariaceae Z.W. de Beer, Begerow& R. Bauer fam. nov., Quambalaria cyanescens (de Hoog & G.A. de Vries) Z.W. de Beer, Begerow & R. Bauer comb. nov.  相似文献   

9.
Cryphonectria havanensis is a fungus associated with Eucalyptus species in Cuba and Florida (U.S.A.). Until recently, there have been no living cultures of C. havanensis and it has thus not been possible to assess its taxonomic status. Isolates thought to represent this fungus have, however, emerged from surveys of Eucalyptus in Mexico and Hawaii (U.S.A.). Results of this study showed that these isolates represent C. havanensis but reside in a genus distinct from Cryphonectria sensu stricto, which is described here as Microthia. Isolates of an unidentified fungus occurring on Myrica faya in the Azores and Madeira also grouped in Microthia and were identical to other M. havanensis isolates. Cryphonectria coccolobae, a fungus occurring on sea grape (Coccoloba uvifera) in Bermuda and Florida, was found to be morphologically identical to Microthia and is transferred to this genus, but as a distinct species. Surveys for M. coccolobae on sea grape in Florida, yielded a second diaporthalean fungus from this host. This fungus is morphologically and phylogenetically distinct from M. coccolobae and other closely related taxa and is described as Ursicollum fallax gen. et sp. nov. Phylogenetic analyses in this study have also shown that isolates of C. eucalypti, a pathogen of Eucalyptus in South Africa and Australia, group in a clade separate from all other groups including that representing Cryphonectria sensu stricto. This difference is supported by the fact that Cryphonectria eucalypti has ascospore septation different to that of all other Cryphonectria species. A new genus, Holocryphia, is thus erected for C. eucalypti.Taxonomic novelties: Microthia Gryzenh. & M.J. Wingf. gen. nov., Microthia havanensis (Bruner) Gryzenh. & M.J. Wingf. comb. nov., Microthia coccolobae (Vizioli) Gryzenh. & M.J. Wingf. comb. nov., Holocryphia Gryzenh. & M.J. Wingf. gen. nov., Holocryphia eucalypti (M. Venter & M.J. Wingf.) Gryzenh. & M.J. Wingf. comb. nov., Ursicollum Gryzenh. & M.J. Wingf. gen. nov., Ursicollum fallax Gryzenh. & M.J. Wingf. sp. nov.  相似文献   

10.
The present study compares all known species of Cylindrocladium that have clavate vesicles. Several isolates were obtained from baited soils collected in various parts of the world, while others were associated with leaf litter or symptomatic plant hosts. Isolates were compared based on morphology, as well as DNA sequence data from their β-tubulin and histone gene H3 regions. Cylindrocladium australiense and Cy. ecuadoriae, are described as new species, a decision based on morphology and molecular data. A group of isolates associated with toppling disease of banana in the West Indies is identified as Cy. flexuosum. An epitype is designated for Cy. ilicicola, and a new name, Curvicladiella, proposed to replace the anamorphic genus Curvicladium, which is a homonym.Taxonomic novelties: Cylindrocladium australiense Crous& K.D. Hyde sp. nov., Cylindrocladium ecuadoriae Crous & M.J. Wingf. sp. nov., Curvicladiella Decock & Crous nom. nov., Curvicladiella cignea (Decock & Crous) Decock & Crous comb. nov.  相似文献   

11.
The classification of Pleosporales has posed major challenges due to the lack of clear understanding of the importance of the morphological characters used to distinguish between different groups in the order. This has resulted in varied taxonomic treatments of many families in the group including Melanommataceae and Lophiostomataceae. In this study we employ two nuclear DNA gene markers, nuclear ribosomal large subunit DNA and translation elongation factor 1-alpha in order to examine the molecular phylogenetics of Pleosporales with strong emphasis on the families Melanommataceae and Lophiostomataceae. Phylogenetic analyses recovered Melanommataceae, Lophiostomataceae, Hypsostromataceae, and a few others as strongly supported clades within the Pleosporales. Melanommataceae as currently circumscribed was found to be polyphyletic. The genera Byssosphaeria, Melanomma, and Pseudotrichia were recovered within the family, while others such as Ostropella and Xenolophium nested outside in a weakly supported group along with Platystomum compressum and Pseudotrichia guatopoensis that may correspond to the family Platystomaceae. The genus Byssosphaeria was recovered as a strongly supported group within the Melanommataceae while Melanomma was weakly supported with unclear relationships among the species. The genera Herpotrichia and Bertiella were also found to belong in the Melanommataceae. Lophiostomataceae occurs as a strongly supported group but its concept is here expanded to include a new genus Misturatosphaeria that bears morphology traditionally not known to occur in the family. The strongly supported clade of Misturatosphaeria contains nine species that have gregarious, papillate ascomata with lighter coloured apices and plugged ostioles and that vary in ascospore morphology from 1- to 3-septate to muriform. Along with a strongly supported Lophiostoma clade, also within the family are Thyridaria macrostomoides based on new sequences from Kenyan collections and Massariosphaeria triseptata, M. grandispora, Westerdykella cylindrica and Preussia terricola based on GenBank sequences. The family Hypsostromataceae was recovered as a strongly supported monophyletic group nested within the Pleosporales.Taxonomic novelties: Misturatosphaeria Mugambi & Huhndorf, gen. nov., M. aurantonotata Mugambi & Huhndorf, sp. nov., M. claviformis Mugambi & Huhndorf, sp. nov., M. cruciformis Mugambi & Huhndorf, sp. nov., M. kenyensis Mugambi & Huhndorf, sp. nov., M. minima Mugambi, A.N. Mill. & Huhndorf, sp. nov., M. tennesseensis Mugambi, A.N. Mill. & Huhndorf, sp. nov., M. uniseptata Mugambi, A.N. Mill. & Huhndorf, sp. nov., M. uniseriata Mugambi, A.N. Mill. & Huhndorf, sp. nov.  相似文献   

12.
Coniosporium epidermidis sp. nov. is described from a superficial skin lesion with blackish discolouration in an 80-yr-old Chinese patient. The species produces dark, thick-walled, inflated, reluctantly liberating arthroconidia without longitudinal septa. Sequences of the ribosomal operon, as well as of the translation elongation factor 1-α support its novelty. The species is found in a lineage basal to the order Chaetothyriales, amidst relatives from rock, but also species repeatedly isolated from human skin and nails and eventually causing mild cutaneous infections. Coniosporium epidermidis is consistently found on humans, either asymptomatic or symptomatic. The species indicates a change of life style towards human pathogenicity, which is a recurrent type of ecology in derived Chaetothyriales. Superficial and cutaneous infection by melanized fungi is a new category in dermatology.Taxonomic novelties: Coniosporium epidermidis D.M. Li, de Hoog, Saunte & X.R. Chen, sp. nov.  相似文献   

13.
Ophiostoma represents a genus of fungi that are mostly arthropod-dispersed and have a wide global distribution. The best known of these fungi are carried by scolytine bark beetles that infest trees, but an interesting guild of Ophiostoma spp. occurs in the infructescences of Protea spp. native to South Africa. Phylogenetic relationships between Ophiostoma spp. from Protea infructescences were studied using DNA sequence data from the β-tubulin, 5.8S ITS (including the flanking internal transcribed spacers 1 and 2) and the large subunit DNA regions. Two new species, O. phasma sp. nov. and O. palmiculminatum sp. nov. are described and compared with other Ophiostoma spp. occurring in the same niche. Results of this study have raised the number of Ophiostoma species from the infructescences of serotinous Protea spp. in South Africa to five. Molecular data also suggest that adaptation to the Protea infructescence niche by Ophiostoma spp. has occurred independently more than once.Taxonomic novelties: Ophiostoma phasma Roets, Z.W. de Beer& M.J. Wingf. sp. nov., Ophiostoma palmiculminatum Roets, Z.W. de Beer & M.J. Wingf. sp. nov.  相似文献   

14.
15.
16.
The morphological concept of Trichoderma koningii is found to include several species that differ from each other in details of phenotype (including conidium morphology, growth rate) and biogeography. Phylogenetic analysis utilizing partial sequences of the translation-elongation factor 1 alpha (tef1), as well as fragments of actin and calmodulin genes, indicate that phenotypic characters typical of T. koningii evolved independently in three well-separated main lineages. Combined molecular and phenotype data lead to the development of a taxonomy with the recognition of twelve taxonomic species and one variety within the three lineages. These lineages include: (1) T. koningii and T. ovalisporum and the new species T. caribbaeum var. caribbaeum, T. caribbaeum var. aequatoriale, T. dorotheae, T. dingleyae, T. intricatum, T. koningiopsis, T. petersenii and T. taiwanense; (2) the new species T. rogersonii and T. austrokoningii, and (3) the new anamorph T. stilbohypoxyli.Trichoderma koningii s. str. is an uncommon species restricted to Europe and eastern North America; T. caribbaeum var. aequatoriale, T. koningiopsis, and T. ovalisporum were isolated as endophytes of trunks of Theobroma species in tropical America, and T. ovalisporum from the woody liana Banisteropsis caapi in Ecuador; T. koningiopsis is common in tropical America but was isolated also from natural substrata in East Africa, Europe and Canada, and from ascospores in eastern North America, and as an endophyte in Theobroma species; T. stilbohypoxyli, originally described as a parasite of Stilbohypoxylon species in Puerto Rico, is found to be more common in the tropics, besides an endophytic isolate from Fagus in U.K. The additional new species are known almost exclusively from their teleomorphs. Isolates of T. ovalisporum and T. koningiopsis may have biological control potential. A morphophenetic key and a set of tools for molecular species identification were developed.Taxonomic novelties:Trichoderma austrokoningii/Hypocrea austrokoningii Samuels & Druzhinina sp.nov., T. caribbaeum var caribbaeum/H. caribbaea Samuels& Schroers sp.nov., T. caribbaeum var. aequatoriale Samuels & H.C. Evans var.nov., T. dingleyae/H. dingleyae Samuels & Dodd sp.nov., T. dorotheae/H. dorotheae Samuels & Dodd sp.nov., T. intricatum/H. intricata Samuels & Dodd sp.nov., T. koningiopsis/H. koningiopsis Samuels, C. Suarez& H.C. Evans sp.nov., T. petersenii/H. petersenii Samuels, Dodd & Schroers sp.nov., T. rogersonii/H. rogersonii Samuels sp.nov., T. stilbohypoxyli Samuels & Schroers sp.nov., T. taiwanense/H. taiwanensis Samuels & M.L. Wu sp.nov.  相似文献   

17.
Ophiostoma species have diverse morphological features and are found in a large variety of ecological niches. Many different classification schemes have been applied to these fungi in the past based on teleomorph and anamorph features. More recently, studies based on DNA sequence comparisions have shown that Ophiostoma consists of different phylogenetic groups, but the data have not been sufficient to define clear monophyletic lineages represented by practical taxonomic units. We used DNA sequence data from combined partial nuclear LSU and β-tubulin genes to consider the phylogenetic relationships of 50 Ophiostoma species, representing all the major morphological groups in the genus. Our data showed three well-supported, monophyletic lineages in Ophiostoma. Species with Leptographium anamorphs grouped together and to accommodate these species the teleomorph-genus Grosmannia (type species G. penicillata), including 27 species and 24 new combinations, is re-instated. Another well-defined lineage includes species that are cycloheximide-sensitive with short perithecial necks, falcate ascospores and Hyalorhinocladiella anamorphs. For these species, the teleomorph-genus Ceratocystiopsis (type species O. minuta), including 11 species and three new combinations, is re-instated. A third group of species with either Sporothrix or Pesotum anamorphs includes species from various ecological niches such as Protea infructescences in South Africa. This group also includes O. piliferum, the type species of Ophiostoma, and these species are retained in that genus. Ophiostoma is redefined to reflect the changes resulting from new combinations in Grosmannia and Ceratocystiopsis. Our data have revealed additional lineages in Ophiostoma linked to morphological characters. However, these species are retained in Ophiostoma until further data for a larger number of species can be obtained to confirm monophyly of the apparent lineages.Taxonomic novelties: Ceratocystiopsis manitobensis (J. Reid& Hausner) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., Cop. parva (Olchow. & J. Reid) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., Cop. rollhanseniana (J. Reid, Eyjólfsd. & Hausner) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., Grosmannia abiocarpa (R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. aenigmatica (K. Jacobs, M.J. Wingf. & Yamaoka) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. americana (K. Jacobs& M.J. Wingf.) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. aurea (R.C. Rob. & R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. cainii (Olchow. & J. Reid) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. clavigera (R.C. Rob. & R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. crassivaginata (H.D. Griffin) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. cucullata (H. Solheim) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. davidsonii (Olchow. & J. Reid) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. dryocoetidis (W.B. Kendr.& Molnar) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. europhioides (E.F. Wright & Cain) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. francke-grosmanniae (R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. galeiformis (B.K. Bakshi) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. grandifoliae (R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. huntii (R.C. Rob.) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. laricis (K. van der Westh., Yamaoka & M.J. Wingf.) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. leptographioides (R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. olivacea (Math.-Käärik) Zipfel, Z.W. de Beer& M.J. Wingf. comb. nov., G. pseudoeurophioides (Olchow. & J. Reid) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. radiaticola (J.-J. Kim, Seifert, & G.-H. Kim) Z.W. de Beer & M.J. Wingf. comb. nov., G. robusta (R.C. Rob. & R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. sagmatospora (E.F. Wright & Cain) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. vesca (R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov., G. wageneri (Goheen & F.W. Cobb) Zipfel, Z.W. de Beer & M.J. Wingf. comb. nov.  相似文献   

18.
Using media with low water activity, a large numbers of aureobasidium-like black yeasts were isolated from glacial and subglacial ice of three polythermal glaciers from the coastal Arctic environment of Kongsfjorden (Svalbard, Spitsbergen), as well as from adjacent sea water, sea ice and glacial meltwaters. To characterise the genetic variability of Aureobasidium pullulans strains originating from the Arctic and strains originating pan-globally, a multilocus molecular analysis was performed, through rDNA (internal transcribed spacers, partial 28 S rDNA), and partial introns and exons of genes encoding β-tubulin (TUB), translation elongation factor (EF1α) and elongase (ELO). Two globally ubiquitous varieties were distinguished: var. pullulans, occurring particularly in slightly osmotic substrates and in the phyllosphere; and var. melanogenum, mainly isolated from watery habitats. Both varieties were commonly isolated from the sampled Arctic habitats. However, some aureobasidium-like strains from subglacial ice from three different glaciers in Kongsfjorden (Svalbard, Spitsbergen), appeared to represent a new variety of A. pullulans. A strain from dolomitic marble in Namibia was found to belong to yet another variety. No molecular support has as yet been found for the previously described var. aubasidani. A partial elongase-encoding gene was successfully used as a phylogenetic marker at the (infra-)specific level.Taxonomic novelties: Aureobasidium pullulans var. subglaciale Zalar, de Hoog & Gunde-Cimerman, var. nov.; Aureobasidium pullulans var. namibiae Zalar, de Hoog & Gunde-Cimerman, var. nov.  相似文献   

19.
Species of Eucalyptus are widely planted as exotics in the tropics and Southern Hemisphere and to some extent in southern Europe, for timber and fibre production. Species of Mycosphaerella are commonly associated with leaves and twigs of Eucalyptus and can result in defoliation, dieback, and even tree death. In the present study, numerous isolates of Mycosphaerella species were collected from leaf litter, living leaves exhibiting leaf spot symptoms or severe Mycosphaerella leaf blotch symptoms. Isolates were compared based on DNA sequence data for the internal transcribed spacer region (ITS1 & ITS2) and the 5.8S gene. These data, together with characteristics of the fungal growth on three different media, morphology of the anamorph and teleomorph structures as well as ascospore germination patterns were used to describe 21 new species.Taxonomic novelties: Colletogloeopsis stellenboschiana Crous sp. nov., Mycosphaerella davisoniellae Crous sp. nov. (anamorph Davisoniella eucalypti H.J. Swart), Mycosphaerella eucalyptorum Crous & M.J. Wingf. sp. nov. Mycosphaerella gamsii Crous sp. nov., Mycosphaerella perpendicularis Crous& M.J. Wingf. sp. nov., Mycosphaerella pluritubularis Crous & J.P. Mansilla sp. nov., Mycosphaerella pseudafricana Crous & T. Coutinho sp. nov., Mycosphaerella pseudocryptica Crous sp. nov. (anamorph Colletogloeopsis sp.), Mycosphaerella pseudoendophytica Crous & G. Hunter sp. nov. (anamorph Pseudocercosporella sp.), Mycosphaerella pseudosuberosa Crous & M.J. Wingf. sp. nov. (anamorph Trimmatostroma sp.), Mycosphaerella quasicercospora Crous & T. Coutinho sp. nov., Mycosphaerella scytalidii Crous & M.J. Wingf. sp. nov. (anamorph Stenella sp., synanamorph, Scytalidium-like.), Mycosphaerella secundaria Crous & A.C. Alfenas sp. nov., Mycosphaerella stramenti Crous & A.C. Alfenas sp. nov., Mycosphaerella stramenticola Crous & A.C. Alfenas sp. nov., Mycosphaerella sumatrensis Crous & M.J. Wingf. sp. nov., Mycosphaerella verrucosiafricana Crous & M.J. Wingf. sp. nov., Septoria eucalyptorum Crous sp. nov., Septoria provencialis Crous sp. nov., Stenella pseudoparkii Crous & M.J. Wingf. sp. nov. (teleomorph Mycosphaerella sp.), Stenella xenoparkii Crous & M.J. Wingf., sp. nov. (teleomorph Mycosphaerella sp.).  相似文献   

20.
Eight pestalotioid fungi were isolated from the Restionaceae growing in the Cape Floral Kingdom of South Africa. Sarcostroma restionis, Truncatella megaspora, T. restionacearum and T. spadicea are newly described. New records include Pestalotiopsis matildae, Sarcostroma lomatiae, Truncatella betulae and T. hartigii. To resolve generic affiliations, phylogenetic analyses were performed on ITS (ITS1, 5.8S, ITS2) and part of 28S rDNA. DNA data support the original generic concept of Truncatella, which encompasses Pestalotiopsis species having 3-septate conidia. The genus Sarcostroma is retained as separate from Seimatosporium.Taxonomic novelties: Pestalotiopsis matildae (Richatt) S. Lee & Crous comb. nov., Truncatella betulae (Morochk.) S. Lee& Crous comb. nov., Sarcostroma restionis S. Lee & Crous sp. nov., Truncatella megaspora S. Lee & Crous sp. nov., Truncatella restionacearum S. Lee & Crous sp. nov., Truncatella spadicea S. Lee & Crous sp. nov.  相似文献   

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