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为切实做好云南省小反刍兽疫消灭工作,保障羊产业稳定健康发展,依据《国家中长期动物疫病防治规划(2012—2020年)》和《全国小反刍兽疫消灭计划(2016—2020年)》要求,2016—2019年分别采用ELISA、RT-PCR方法,在昆明市开展了小反刍兽疫免疫抗体和病毒核酸检测。结果显示:909个集中监测养殖场点的8972份血清样品中,检出免疫合格场点841个,平均场点免疫合格率为92.5%;检出免疫合格样品7405份,平均个体免疫合格率为82.53%,虽然超过了农业农村部要求的不低于70%的标准,但各年度的个体合格率有下降趋势;规模场和散养户的个体免疫合格率分别为84.97%和82.07%,场点合格率分别为83.33%和93.38%,均无显著差异(P>0.05);不同县(市、区)的个体免疫合格率为77.36~93.17%,场点合格率为83.33%~98.55%,差异均不显著(P>0.05);在3812份羊眼(鼻)棉拭子和组织样品中检出疑似核酸阳性2份,分别来自2个散养户。结果表明:昆明市小反刍兽疫整体防控效果较好,发生大面积流行的风险较低,但仍有散发疫情风险。因此,仍需继续做好小反刍兽疫的强制免疫、监测和流行病学调查工作,同时加强羊只的引种检疫和流通监管,最终到达消灭小反刍兽疫的目标。 相似文献
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为全面掌握云南省玉溪市小反刍兽疫（PPR）的病原分布和羊群免疫效果，根据2021年玉溪市PPR专项监测方案，在全市开展PPR监测和流行病学调查。采用多阶段调查的方法，从全市9个县区采集免疫羊血清样品1 631份、眼鼻棉拭子样品6 438份，分别使用阻断ELISA和实时荧光RT-PCR检测方法，进行PPR免疫抗体检测和病毒核酸检测。结果显示：2021年玉溪市PPR群体免疫合格率为82.98%，个体免疫合格率为83.62%，未检测到病原学阳性样品；不同养殖区域、不同性别的免疫抗体合格率均有统计学差异（P＜0.05），而不同养殖类型、不同企业疫苗的免疫抗体合格率差异均不显著（P＞0.05）。结果表明：玉溪市羊PPR整体免疫效果较好，所用PPR疫苗免疫原性较好，免疫程序合理，但部分县区的免疫合格率较低，因此应继续加强PPR的强制免疫及补免工作；在未引入新疫源的前提下，疫情发生风险较低，但应加大羊只引种检疫和调运监管力度。本次监测评估了玉溪市的PPR总体防控状况，为尽快实现消灭PPR的目标提供了数据支撑。 相似文献
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为掌握山东省淄博市小反刍兽疫免疫效果,2019年用估计流行率方法,从全市规模养殖场和散养户中抽取养殖场户,按比例分配到各区县;在养殖场户内,随机采集血清样品,采用竞争ELISA方法进行小反刍兽疫抗体检测,并进行不同类型场点和不同地区的统计对比。结果显示:共检测111个养殖场点,检出免疫合格场点106个,群体免疫合格率为95.50%;共检测3 345份血清样品,检出免疫合格样品3 026份,个体免疫合格率为90.46%。散养户个体免疫合格率(94.71%)和群体免疫合格率(96.92%)略高于规模场(86.36%、93.48%),但差异均不显著(P 0.05)。从不同地区看,经济技术开发区个体免疫合格率最低(74.26%),而其他地区均在82.00%以上;周村区群体合格率最低(87.50%),而其他地区均在90.00%以上。结果表明:淄博市小反刍兽疫整体免疫效果较好,疫情发生风险较低,但免疫效果较差养殖场户仍要加强小反刍兽疫免疫,规模场要加强引入羊群的检疫隔离,防止因病原引入而暴发疫情。 相似文献
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为落实国家小反刍兽疫消灭计划,掌握安徽省小反刍兽疫流行趋势与免疫状况,2017—2019年,每年从安徽省16个地市、2个直管县的种羊场、商品代羊场和散养户抽取不同数量的羊血清和鼻拭子样品,分别用竞争ELISA、荧光RT-PCR方法检测小反刍兽疫免疫抗体与病毒核酸。3年共抽样检测842个场点的15 943份血清学样品,平均免疫个体合格率为88.16%、场点合格率为89.31%,每年的个体合格率和场点合格率均在84%以上;检测12 411份病原学样品,未检测出阳性样品。但芜湖市3年的平均个体免疫合格率仅为68.24%,淮南市平均场点合格率仅为65.28%,未达到农业农村部要求的70%以上的标准。结果表明,安徽省小反刍兽疫整体免疫效果较好,疫情暴发风险较低,基本达到了免疫无疫标准,但仍要关注免疫效果较差地区的免疫、监测与流行病学调查,加大调运监管力度,降低疫情发生风险。 相似文献
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为了解2019年河南省猪群猪繁殖与呼吸综合征（PRRS）免疫抗体水平和病原流行情况，对河南省3 231场次的108 070份猪血清和714场次的18 746份猪组织样品，分别进行免疫抗体和病原学检测，并按季节、区域和场点类别，对检测结果进行了统计分析。抗体检测结果显示：2019年河南省平均个体免疫合格率为83.53%，场群免疫合格率为90.75%；不同季节、不同区域和不同场群的抗体水平均在70%以上，但散养户（70.21%）水平偏低。病原检测结果显示：2019年河南省个体病原阳性率为0.99%，场群病原阳性率为4.06%；一年四季均有病原检出，且春季阳性率最高；豫北地区个体和场群的病原阳性率均最高，而豫西地区未检出病原；无害化处理厂和散养户的病原阳性率较高。结果表明：河南省猪群PRRS免疫效果良好，但应加强散养户猪群的免疫；病原具有一定的时间、空间和群间分布特征，可依据其分布特点和规律，分类指导防控，以种猪场为中心，梯度推进净化工作。 相似文献
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《贵州畜牧兽医》2015,(6)
为了解2012—2014年贵州省猪瘟免疫防控情况,采用间接ELISA检测方法,对随机采集我省9个市(州)11 390个场点113 342份经过猪瘟疫苗免疫后的猪血清样品,进行猪瘟免疫抗体检测。结果显示,猪瘟免疫抗体平均合格率为80.92%;2012—2014年猪瘟免疫抗体合格率分别为78.79%、82.48%、82.37%,连续3年均达到国家免疫合格标准70%以上,呈现稳定的上升趋势,且2013、2014年样品免疫抗体合格率显著高于2012年;按照不同季度、不同场点、不同地区进行统计分析,检测样品猪瘟免疫抗体合格率也均达到70%以上。研究结果表明,贵州省近3年猪瘟免疫防控工作较为理想。 相似文献
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为了解山东省规模猪场的猪瘟免疫效果及变化趋势,2011—2019年在16个地市717个规模猪场,采集血清样品22 465份,采用猪瘟抗体ELISA方法进行抗体检测,并比对不同年份和不同场点的免疫水平。结果显示:2011—2019年,全省16个地市种猪场的场群免疫合格率为73.84%,个体合格率为77.54%;商品代猪场的场群免疫合格率为63.96%,个体免疫合格率为71.79%。从不同年份看,规模猪场的猪瘟免疫抗体水平呈波浪状变化,2011—2012年免疫水平较高,2013—2014年较低,2015—2016年再次升高,2017—2019年免疫水平又出现下降;从不同场点看,无论是个体免疫效果,还是群体免疫效果,种猪场均优于商品代猪场。结果表明,山东省规模猪场的总体猪瘟免疫抗体水平不高且不稳定,因而规模猪场应持续加强猪瘟的免疫和监测,尤其是商品代猪群,以降低猪瘟的发生风险。 相似文献
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了解都匀市猪瘟抗体水平及病毒分布情况,为后续免疫工作开展提供数据支持。利用ELISA检测方法,对2020—2022年间种畜场、商品代场、散养户3种类型检测场点进行监测,共收集血清样品3326份,合格样品数为3232份,总体合格率为97.17%,并且3年内检测结果均在90%以上,其中2020年为96.77%、2021年为98.70%、2022年为93.58%,均在农业农村部规定的70%合格率以上。另外,在3种类型检测场点中,种畜场的合格率最高,为98.92%,散养户的合格率居中,为94.49%,商品代场的合格率较低,为92.95%。结果表明：都匀市CSF免疫及疫情防控工作实施效果较好,但后续仍需加强宣传、严把疫苗存储关、加强督查指导,为都匀市猪肉食品健康保驾护航。 相似文献
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为全面了解2017—2021年云南省猪瘟(CSF)流行及免疫状况,预估疫病流行和发生风险,采集病原学样品63 224份、血清学样品146 547份,进行CSF病原和血清抗体监测。结果显示:2017—2021年云南省CSF免疫抗体合格率分别为82.94%、81.47%、80.79%、80.24%、82.53%,规模场合格率略高于散养户,但差异不显著(P>0.05);CSF病原核酸阳性率分别为0.52%、0.74%、1.10%、1.02%、2.42%,散养户、屠宰场阳性率高于规模场。结果表明:自2017年CSF退出强制免疫后,云南省CSF免疫抗体合格率均达到国家标准,但病原阳性率整体呈上升趋势,且分布范围广,免疫密度低和抗体合格率低的散养户发生CSF的风险较高,提示应对其加强监测和免疫。 相似文献
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
相似文献12.
Heartworm in dogs in Canada in 1982 总被引：8，自引：8，他引：0
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1983,24(7):227-229
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献14.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献16.
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Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
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In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献19.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献