共查询到19条相似文献，搜索用时 453 毫秒
1.
利用ISSR分子标记技术对14个居群的红花石蒜进行遗传多样性研究,结果表明:POPGEN32分析显示红花石蒜物种的遗传多样性很高,多态位点百分率为92.31%,Shannon指数(H)为0.459 7,Ne i指数(I)为0.302 5;居群水平的遗传多样性较低,多态位点百分率平均为49.65%,Shannon指数(H)平均为0.262 0,Ne i指数(I)平均为0.176 3;居群间的遗传分化系数(Gst)为0.503 5,基因流(Nm)为0.698 3。AMOVA分子变异分析显示:居群间遗传分化程度高,46.12%的变异发生在居群内,53.88%的变异发生于居群间。生境的片段化使居群间的基因流受阻,可能是居群间高遗传分化和居群水平低遗传多样性的主要原因。 相似文献
2.
3.
美国白蛾种群的遗传多样性与遗传分化 总被引：3，自引：0，他引：3
采用微卫星技术对入侵中国的5个美国白蛾种群进行遗传多样性和遗传分化及影响因素的研究.用10对微卫星引物对300个样品DNA进行PCR扩增,并进行聚丙烯酰胺凝胶电泳分析.5个美国白蛾种群的多态位点百分率P=98.36%;多态位点百分率的大小顺序为:沧州种群、烟台种群、秦皇岛种群、丹东种群、石家庄种群;美国白蛾整个群体的遗传分化:34.38%的变异存在于种群间,65.62%的变异存在于种群内,遗传距离变化范围为0.138 6～0.322 4.用UPGMA法对Nei's遗传距离作聚类分析的结果表明:种群的分化程度与分化时间和入侵来源密切相关.影响美国白蛾种群遗传多样性与遗传分化的主要因素是经度、平原比率、年降水量、纬度、入侵时间.美国白蛾种群现有遗传结构的形成与其生活史特性及入侵生态学特性有关. 相似文献
4.
利用AFLP分子标记技术对筇竹2个种群的遗传多样性进行了分析,筛选出10对多态性和清晰度较高的引物组合,共获得680个AFLP位点,其中多态性位点662个,平均多态性检出率为97.20%,并用PopGen32软件对AFLP多态性数据进行分析,结果表明,供试2个筇竹种群均具有丰富的遗传多样性,多态位点百分率分别为89.86％和91.95％,等位基因数分别为1.898 6和1.919 5,有效等位基因数分别为1.585 0和1.568 3,种群内遗传多样性为0.332 1,种群水平平均Nei’s 遗传多样性为0.394 9,Shannon信息指数为0.575 4,基因流为2.900 9,遗传一致度为0.821 9,遗传距离平均值为0.196 1。并根据遗传距离进行了UPGMA聚类分析。 相似文献
5.
利用简单重复序列间扩增(ISSR)分子标记对分布于我国浙江和山东的8个山茶种群共240个个体进行了遗传结构分析。结果表明:筛选出的20条引物扩增得到210条清晰条带,其中184条为多态性条带,多态位点百分率(PPB)为87.62%。经POPGENE1.32软件分析,山茶种群平均多态位点百分率(PPB)为68.99%,Nei’s基因多样性指数(HE)为0.256 9,Shannon信息指数(H)为0.377 9,种群遗传多样性较高。基因分化系数Gst=0.182 9,表明遗传变异主要存在于种群内个体间。地理距离与遗传距离具有显著相关性(r=0.856 7,P<0.05),岛屿地理隔离对山茶种群的遗传分化具有重要影响。UPGMA聚类表明:浙江5个山茶种群间的遗传相似度较高,山东青岛的植物园种群和五四广场种群可能移植自长门岩岛。我国野生山茶资源受人为破坏严重,建议在加强现有岛屿自然种群就地保护力度的同时,建立山茶种质资源库,促进基因交流。 相似文献
6.
七子花种群遗传多样性的RAPD分析 总被引：6，自引：0，他引：6
按胸径将浙江省天台山天然七子花划分为大树、中树、小树和幼树 4个大小级种群 ,利用RAPD分子标记技术对 4个大小级种群共 4 5株个体的遗传多样性及遗传分化进行了比较分析 ,结果表明 :通过 12种随机引物扩增共检测到 6 4个可重复的位点 ,4个大小级种群的多态位点百分率高低为大树 >中树 >小树 >幼树。Shannon信息指数估计七子花 4个大小级种群的遗传多样性高低为大树 >中树 >小树 >幼树 ,大小级种群内的遗传变异占总变异的 5 4 4 1% ,大小级种群间的遗传变异占总变异的 4 5 5 9% ,表明不同大小级种群内、大小级种群间个体均存在一定的遗传分化 ,但大小级种群内的遗传分化比大小级种群间高。Nei指数估计的七子花不同大小级种群的基因多样性的高低与Shannon指数估计的一致 ,大小级种群内的遗传分化高于大小级种群间 ,大小级种群间的基因分化系数为 0 3939。不同大小级种群间的遗传相似度以大树与中树最高 ,大树与幼树最低。聚类分析显示大树与中树先聚成一组 ,小树与幼树再聚成一组 ,最后 2组聚在一起 相似文献
7.
湖南桂阳马尾松种子园遗传多样性的ISSR分析 总被引：2，自引：0，他引：2
本研究从100条ISSR引物中筛选出11条多态性引物,对湖南桂阳马尾松第二代种子园共87份样品进行遗传多样性分析,共扩增得到246个多态位点。采用PAUP4.0软件分析,获得两个主要群体(广西群体和城步群体),在城步群体内,湖南和贵州两省的家系被分开。再对两个主要群体采用POPGEN32软件分析,结果表明:在桂阳第二代种子园内总的多态位点百分率达98.80%,总的Shannon信息指数为0.583 7,Nei's基因多样性指数为0.400 3,2个群体的多态位点百分率分别为93.57%、98.80%,各群体间的基因分化系数为0.036 3,基因流为13.258 6。这表明桂阳第二代马尾松种子园的遗传多样性水平高,个体间的基因多样性变异大,该种子园所采用的建园方式是可取的。 相似文献
8.
《中南林业科技大学学报(自然科学版)》2016,(5)
采用ISSR分子标记技术,对我国濒危植物福建柏的7个种群进行遗传多样性分析。结果显示:17个引物共扩增出202个位点,多态性位点167个,占总位点的82.67%;引物UBC849可以将24份福建柏样本完全鉴别出来;7个福建柏种群的多态位点百分率在17.82%~54.95%之间;群体总观测等位基因数Na为1.821 8,有效等位基因数Ne为1.431 3,Nei指数为0.258 2,Shannon指数为0.394 0;种群内平均Na指数为1.372 0,平均Ne指数为1.289 2,平均Nei指数为0.160 0,平均Shannon指数为0.203 5;基因分化系数Gst为32.41%,种群间基因流Nm为0.878 0;7个种群遗传相似度在0.668 3~0.806 9之间,遗传距离在0.214 5~0.404 3之间;利用UPGMA法进行聚类,将泉州市、福州市和龙岩市种群分为第一类,将南平市、三明市和漳州市种群分为第二类,将宁德市种群分为第三类。 相似文献
9.
[目的]研究南方皂荚的遗传多样性和遗传结构现状,为制定有效的保护策略和育种策略提供理论依据。[方法]采用扩增片段长度多态性技术对供试的215份皂荚的遗传多样性及遗传距离进行分析。[结果]表明:检测到1 782个位点,其中,有1 389个为多态性位点,多态位点百分率为77.94%;皂荚各群体Shannon’s信息指数平均为0.256;Nei’s多样性指数平均为0.168,表明皂荚群体的遗传多样性偏低,皂荚群体间存在着一定的遗传分化;根据遗传距离UPGMA聚类分析,将10个群体划分为4大类。由遗传分化系数和分子方差分析结果发现,群体内的变异是皂荚遗传变异的主体,74.79%的变异来自群体内。[结论]皂荚群体的遗传多样性与遗传结构的形成不仅与其分布广泛、种子特性及生活史有关,而且与人为的砍伐、引种、生境片段化等因素有重要关系。基于上述结果提出了皂荚的保护策略。 相似文献
10.
大花黄牡丹遗传多样性的SRAP分析 总被引：2，自引：0，他引：2
应用SRAP标记对西藏特有植物大花黄牡丹的遗传多样性进行研究。用16对引物从5个自然居群79个单株中共检测到396个有效位点,其中多态性位点357个。在物种水平上,多态位点百分率(Ppl)为90.15%,Shannon表型多样性指数(Ηsp)平均为0.2521;居群水平上的Ppl为31.82%,Shannon表型多样性指数(Ho)为0.0694～0.3428,平均值(Ηpop)为0.1307。上述遗传参数表明,大花黄牡丹具有丰富的物种遗传多样性,5个居群中自然居群C的遗传多样性最高(Ppl=82.32%,Ho=0.3428)。据AMOVA分析结果,总的变异中有41.58%的变异存在于居群间,58.42%的变异存在于居群内,居群分化较显著(ΦST=0.4158,P<0.001),由POPGENE1.32得到的居群间遗传分化系数GST(0.4309)和Shannon表型多样性指数计算的居群间遗传多样性所占比例(0.4816)也表明了类似的遗传结构。Mantel检测表明地理距离和Nei’s遗传距离间相关不显著(P>0.05)。利用NTSYSPC(2.1)软件构建大花黄牡丹5个居群79个个体的UPGMA聚类图,遗传相似系数变幅在0.47～0.99,大多数居群内的个体表现出较为密切的亲缘关系(如居群B,D,E),但也有一些居群的个体未聚在一起(如居群C)。依据大花黄牡丹居群遗传变异特点,初步探讨其保护和利用策略。 相似文献
11.
In order to understand the relationship between population succession and its genetic behavior, random amplified polymorphic
DNA (RAPD) technique was used to analyze the genetic diversity of Quercu glandulifera var. brevipetiolata populations in three forest communities with different succession stages (coniferous forest, coniferous and broad-leaved
mixed forest, evergreen broad-leaved forest). The results showed that 145 repetitive loci were produced in 60 individuals
of Q. glandulifera using 11 primers, among which 120 loci were polymorphic, and the total percentage of polymorphic loci was 82.76% with an
average of 64.14%. Estimated by the Shannon information index, the total genetic diversity of the three populations was 0.4747,
with an average of 0.3642, while it was 0.3234, with an average of 0.2484, judged from the Nei index. Judged from percentage
of polymorphic loci, Shannon inform at ion index and Nei index, the genetic diversity followed a decreasing order: coniferous
forest > broad-leaved mixed forest > evergreen broad-leaved forest. Analysis of molecular variance (AMOVA) showed that 69.73%
of the genetic variance existed within populations and 30.27% of the genetic variance existed among populations. The coefficient
of gene differentiation (GST) was 0.2319 and the gene flow (N
m) was 1.6539. The mean of genetic identity among populations of Q. glandulifera was 0.8501 and the mean of genetic distance was 0.1626. The genetic identity between the Q. glandulifera population in the coniferous forest and that in the coniferous and broadleaved mixed forest was the highest. UPGMA cluster
analysis based on Nei’s genetic distance showed that the population in the coniferous forest gathered with that in the coniferous
and broad-leaved mixed forest firstly, then with that in the evergreen broad-leaved forest. The genetic structure of Q. glandulifera was not only characteristic of the biological characteristics of this species, but was also influenced by the microenvironment
in different communities.
__________
Translated from Journal of Northwest Forestry University, 2008, 23(1): 18–22 [译自: 西北林学院学报] 相似文献
12.
天然蒙古栎群体遗传多样性的RAPD分析 总被引：21，自引：3，他引：21
蒙古栎 (ＱｕｅｒｃｕｓｍｏｎｇｏｌｉｃａＦｉｓｃｈ)为壳斗科、栎属植物 ,主要分布区包括中国的华北、东北、内蒙古东部 ,朝鲜半岛 ,俄罗斯远东和蒙古的连续分布区及日本桦太、北海道的间断分布区 (吴晓春 ,1 993)。蒙古栎是我国东北的典型植被类型———红松阔叶林的主要伴生树种之一 ,同时在红松林被干扰破坏后是形成次生阔叶林面积较大的主要树种 ,也是分布区超过北纬 45°的唯一栎属植物。近年来 ,蒙古栎的分布区和群体数量呈扩张趋势 ,其原因除人为干扰外 ,可能涉及气候变化、物种特性、种内遗传多样性、种间互作等等。对蒙古栎群体… 相似文献
13.
By using random amplified polymorphic DNA (RAPD) technique, this paper studied the genetic diversity and genetic differentiation
of Lithocarpus harlandii populations in three forest communities (coniferous forest, coniferous and broad-leaved mixed forest, and evergreen broad-leaved
forest) with different succession stages in Tiantai Mountain in Zhejiang Province. The results showed that a total of 173
repetitive loci were produced in 60 individuals of L. harlandii by 12 random primers, among which, 152 loci were polymorphic, and the total percentage of polymorphic loci was 87.86%. The
average percentage of polymorphic loci of the populations was 65.32%, and their total genetic diversity estimated by Shannon
information index was 0.4529, with an average of 0.3458, while that judged from Nei’s index was 0.3004, with an average of
0.2320. The percentage of polymorphic loci, Shannon information index, and Nei’s index of the populations were in the sequence
of coniferous forest community > coniferous and broad-leaved mixed forest community > evergreen broad-leaved forest community.
Analysis of molecular variance (AMOVA) showed that 72.85% of genetic variance was found within the populations, and 27.15%
of genetic variance resided among the populations. The coefficient of gene diferentiation was 0.2277, and the gene flow was
1.6949. The genetic structure of L. harlandii was influenced not only by the biological characteristics of this species, but also by the microenvironment of different
communities. The mean of genetic identity among three populations of L. harlandii was 0.8662, and the mean of their genetic distance was 0.1442. The genetic similarity between coniferous and broad-leaved
mixed forest community and evergreen broad-leaved forest community was the highest, while that between evergreen broad-leaved
forest community and coniferous forest community was the lowest. The unweighted pair group method with arithmeticmean (UPGMA)
cluster analysis based on Nei’s genetic distance showed that conierous and broad-leaved mixed forest community first gathered
with evergreen broad-leaved forest community, and then with coniferous forest community.
__________
Translated from Chinese Journal of Ecology, 2007, 26(4): 509–514 [译自: 生态学杂志] 相似文献
14.
采用随机扩增多态DNA(RAPD)方法对6个群体30丛撑篙竹个体进行了遗传变异的研究。28个随机引物共检测到173个位点,其中85个是多态位点,平均每个引物提供6．18个RAPD信息量,扩增出的DNA片段大小一般在200～2000bp范围之间;用POPGENE1．31版软件进行遗传多样性分析：平均Nei’s基因多样性为0．2114,Shannon’s信息指数为0．3277,基因分化系数0．1853,表明群体间有一定的分化;各群体平均遗传距离0．0350,表明群体亲缘关系较近;试用UPGMA方法对不同产地的撑篙竹群体作聚类分析,初步可将6个群体聚为3类。 相似文献
15.
Hepatacodium miconioides is the Class II protected plant species in China. This paper studies the genetic diversity and differentiation of its nine
natural populations in Zhejiang Province by using random amplified polymorphic DNA (RAPD) technique. Twelve random primers
were selected in the amplification, and 164 repetitive loci were produced. The percentage of polymorphic loci in each H. miconioides population ranged from 14.60% to 27.44%, with an average of 20.73%. Among the test populations, Kuochangshan had the highest
percentage of polymorphic loci, Simingshan took the second place, and Guanyinping had the lowest percentage. As estimated
by Shannon index, the genetic diversity within H. miconioides populations accounted for 27.28% of the total genetic diversity, while that among H. miconioides populations accounted for 72.72%. The genetic differentiation among H. miconioides populations as estimated by Nei index was 0.715,7. This figure was generally consistent with that estimated by Shannon index,
i.e., the genetic differentiation among populations was relatively high, but that within populations was relatively low. The
gene flow among H. miconioides populations was relatively low (0.198,7), and the genetic similarity ranged from 0.655,7 to 0.811,9, with an average of 0.730,6.
The highest genetic distance among populations was 0.422,9, while the lowest was 0.208,3. All the results showed that there
was a distinct genetic differentiation among H. miconioides populations. The genetic distance matrix of nine test populations was calculated using this method, and the clustering analysis
was made using the unweighted pair group method with arithmetic mean (UPGMA). The cluster analysis suggested that the nine
populations of H. miconioides in Zhejiang Province could be divided into two groups, the eastern Zhejiang group and the western Zhejiang group.
__________
Translated from Chinese Journal of Applied Ecology, 2005, 16(5): 795–800 [译自: 应用生态学报, 2005, 16(5): 795–800] 相似文献
16.
以林口青山红松无性系种子园216个单株生健康嫩叶为试验材料,利用ISSR-PCR分子标记技术分析其遗传多样性的试验结果表明:从100条引物中共筛选出11条具有多态性的ISSR引物,经ISSR-PCR扩增后共获得102个位点,多态性谱带102条,多态位点比率为100%,单株有效等位基因数变动范围为1.0046~2.000,群体总基因多样性指数为0.3333,Shannon多样性指数为0.0164~0.6931,期望杂合度为0.0046~0.5000,红松种子园单株具有丰富的遗传多样性。 相似文献
17.
巨桉群体遗传结构分析 总被引：3，自引：0，他引：3
用20个l0bp随机引物对巨桉11个种源80个个体进行了群体遗传变异的比较分析。共检测到149个位点,其中89个为多态性标记位点,总的多态位点百分率为59．73％。其种源内多态位点百分率(P)、香农信息指数(SI)及遗传分化指数(DC)值均较高,巨桉各种源内遗传变异性丰富;种源间遗传分化指数百分比(PDC)显示,PDC值介于1．52％～10．42％之间,说明巨桉种源间遗传变异较小,即种源群体的变异主要存在于群体内部。8号、10号种源内具有较高的遗传变异,3号和6号种源内的变异水平较低。这些为巨桉种源的选择提供了有利的遗传基础。 相似文献
18.
北五味子遗传多样性的ISSR分析 总被引：2，自引：0，他引：2
采用ISSR(Inter Simple Sequence R epeat)标记技术,分析了东北三省16个产地北五味子的遗传多样性.6个ISSR引物共检测到83个位点,多态位点的百分率在9.64%～42.17%之间.ISSR的Shannon指数变化范围在0.0583～0.1966之间,Nei指数的变化范围在0.0399～0.1254之间.16个产地的北五味子的基因多样性为24.05%,产地间变异占总变异的66.81%.各产地的地理气候因子与遗传变异指数相关分析表明,影响遗传多样性的地理气候因子为经度和温度,表现为分布区西部、生长季节温度较低的产地变异丰富的特点. 相似文献
19.
湖北麻城杜鹃古群落遗传多样性研究 总被引：2，自引：1，他引：1
湖北麻城杜鹃是迄今发现的我国最大的古杜鹃原始群落,它不但具有重要的经济观赏价值,而且具有重要的研究价值。利用ISSR分子标记技术对湖北麻城龟山景区的杜鹃古群落遗传多样性进行了初步分析。14个多态引物共检测到182个位点,其中多态位点144个,多态位点百分率为79.12%;平均有效等位基因数为1.470 8个,平均Nei's基因多样性为0.289 3,平均Shannon信息指数为0.447 5,表明该景区杜鹃的遗传多样性较高。 相似文献