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1.

Purpose

In spite of substantial advancement in recent past, the role of metal speciation in assessing biogeochemical behaviour of Pb is still topical. Organic ligands are capable to modify Pb speciation in nutrient/soil solution and in turn its soil–plant transfer and toxicity. In this sense, the main objective of this study was to evaluate the effect of organic ligands on Pb-induced oxidative stress to Vicia faba roots.

Materials and methods

V. faba seedlings grown to controlled hydroponic system were treated with 5 μM Pb as lead nitrate in the presence and absence of organic ligands viz ethylenediaminetetraacetic acid (EDTA) and citric acid (CA) for 1, 4, 8, 12, and 24 h. The chemical speciation of Pb (percent free and chelated Pb) in nutrient solution in the presence and absence of organic ligands was calculated using Visual Minteq speciation model. The effect of chemical speciation on Pb-induced oxidative stress to V. faba roots was investigated using plant enzymatic antioxidative system [superoxide dismutases (SOD), guaiacol peroxidise (GPX), ascorbate peroxidase (APX), glutathione reductase (GR), and catalase (CAT)]. The antioxidant enzymes activities were determined using ultraviolet spectrophotometer.

Results and discussion

The activities of SOD, GPX, APX, and GR significantly increased whereas that of CAT decreased in V. faba roots under Pb alone treatment. Lead-induced increase/decrease in antioxidant enzymes activities was not linear but varies with treatment exposure time. EDTA dose dependently inhibited Pb-induced changes in antioxidant enzymes activities. However, CA did not cause any significant change in Pb-induced variation in antioxidant enzymes activities, but delayed or slightly reduced the Pb effect.

Conclusions

The present study suggested that physiological responses of V. faba roots to Pb toxicity vary with applied Pb form and duration of exposure. EDTA can inhibit Pb-induced toxicity to V. faba seedlings by forming stable Pb-EDTA complexes due to its high binding strength for Pb. However, CA had no effect on Pb-induced toxicity to V. faba roots due to weak complexation with Pb.  相似文献   

2.
Acute toxicity of Pb to the water flea; (Daphnia sp) and Copepod, (Cyclop sp) both important component of zooplankton diet of fish was determined by static assay. A positive relationship between percentage mortality and exposure concentration was found in all tests. Mean 24-h LC50, 48-h LC50 and 96-h LC50 values were 2.51?±?0.0.04 mg l?1, 1.88?±?0.06 mg l?1 and 1.65?±?0.19 mg l?1 for Daphnia spp and 3.11?±?0.03 mg l?1, 2.97?±?0.05 mg l?1 and 2.61?±?0.09 mg l?1 for Cyclop spp, respectively. For all tested species did the LC50 values decrease with time; the decrease was more marked for Daphnia spp. Observed symptoms include spiral movement followed by change of body colour to white and rapid disintegration of the skin. The Daphnia spp. appear to be more sensitive to Pb poison than Cyclop spp. The results showed that concentrations of Lead (Pb) in excess of 0.19 mg l?1 and 0.30 mg l?1 can be potentially harmful to Daphnia magna and Cyclop spp respectively.  相似文献   

3.
The potential anticlastogenic and antitoxic effects of a soil humic acid (HA), a peat HA and a peat fulvic acid (FA) on the mutagen maleic hydrazide (MH) have been investigated in two legume species, Vicia faba and Pisum sativum. Both HAs and FA were tested at two different concentrations, 20 and 200 mg l?1, either alone or after 24‐hour interaction with 10 mg l?1 of MH before addition to the legume seeds. Anticlastogenicity, i.e. an antimutagenic action defined as the capacity for minimizing chromosome breakages, was evaluated by counting both micronuclei (MN) and aberrant anatelophases (AAT) in root‐tip cells. Length and dry weight of the seedling primary root were measured to test the antitoxic activity of HA and FA on MH. The possible occurrence and extent of adsorption or desorption of MH onto or from HA were also investigated. The two species responded differently to the anticlastogenic tests, with V. faba showing a greater number of MN and AAT anomalies than P. sativum. Peat HA and FA exhibited anticlastogenic and antitoxic activities of similar intensity and greater than those of soil HA. The adsorption capacity of both HAs for MH was small, thus suggesting that adsorption is not a major mechanism responsible for the reduction of clastogenicity and antitoxicity of MH by HA.  相似文献   

4.
This study was conducted to obtain information on the properties of the nitrate (NO3 ‐) uptake system in Vicia faba. The results showed that in plants grown in the absence of NO3 ‐, they cannot absorb it upon initial exposure to this anion. When this plant makes contact with environmental NO3 ‐, NO3 ‐uptake by the roots is progressively induced. However, in NO3 ‐‐grown plants, the uptake system is yet induced so they had the ability to take up NO3 ‐ at high rates from the beginning. The Michaelis’ constant (K,M) and maximum uptake rate (Vmax) were estimated from measurements of NO3 ‐ depletion in the uptake medium. The apparent KM value for net NO3 ‐ uptake in induced V. faba plants was 60 μM and the corresponding Vmax 0.44 umol NO3 ‐ (g FW)‐1 h‐1. This last value was low compared with that of other species, while KM is in the range for other higher plants. From the results of our experiments at low and high NO3 ‐ concentrations, we discuss the possibility of the existence of two NO3 ‐ uptake systems, one of a high affinity (functioning at low NO3 ‐concentrations) and another of low affinity (operative at high NO3 ‐ level in the medium).  相似文献   

5.
The effect of sodium chloride (NaCl), sodium sulfate (Na2SO4), and potassium chloride (KCl) on growth and ion concentrations of faba bean (Vicia faba L. cv. Troy) was studied. After 14 or 15 d of isoosmotic treatment with 100 mM NaCl or 75 mM Na2SO4, respectively, plants developed toxicity symptoms. These symptoms were characterized by local and nonchlorotic wilting spots, which later turned to black, necrotic spots. In contrast to NaCl or Na2SO4 treatment, plants treated with 100 mM KCl did not show these symptoms. The symptoms occurred on those leaves that accumulated highest concentrations of Na+ and showed highest Na+ : K+ ratios. Our results indicate that Na+ toxicity inducing K+ deficiency is responsible for the spot necrosis of faba bean. Additionally, chlorotic symptoms occurred. The concentrations of Na+ and Cl– were determined in chlorotic leaves and in isolated chloroplasts. The reduction of chlorophyll in leaves after NaCl exposure may be explained in terms of high Cl– concentrations in the chloroplasts and appears to depend on high Na+ concentrations. Chlorotic toxicity symptoms can be avoided by additional Mg2+ application.  相似文献   

6.
A commercial cultivar (Alborea) of faba bean (Vicia faba L. var. minor) was inoculated with salt-tolerant Rhizobium leguminosarum biovar. viciae strain GRA19 in solution culture with different salt concentrations (0, 50, 75 and 100 mmoles l−1 NaCl) added immediately at the time of inoculation. The results indicated that Rhizobium leguminosarum strain GRA19 formed an infective and effective symbiosis with faba bean under saline and nonsaline conditions. Salinity significantly decreased shoot and root dry weight, nodule weight and mean nodule weight. Roots were more sensitive than shoots, and N2 fixation was more sensitive to salinity than was plant growth. Analyses of ammonium assimilating enzymes in the nodule showed that glutamine synthetase appeared to be more tolerant to salinity than glutamate synthase, and that it limits ammonium assimilation under saline stress.  相似文献   

7.
Abstract

The major purpose of these experiments was to determine if Pb uptake by plants was significantly increased by chelating agents used in plant nutrition. The interaction of Pb with some other elements in barley plants (Hordeum vulgare L. C.V. Atlas 57) and bush bean (Phaseolus vulgaris L. C.V. Improved Tendergreen) was studied in a glasshouse with different rates of Pb in solution culture and in amended (control, S, CaCO3, MgCO3) Yolo loam soil with and without the chelating agent DTPA (diethylene triamine pentaacetic acid). In a solution culture experiment, 10‐3 M Pb significantly decreased bush bean yields in both control and DTPA treatments. The CaCO3 added to nutrient solution decreased the concentration of Pb in leaves, stems, and roots and prevented the toxicity of 10‐3 M Pb++. At high Pb levels, interactions between Pb and Mn and Pb and Fe were observed, except with CaCO3. In the soil experiment, the yields of barley and bush bean were influenced only slightly by Pb. The Pb concentration in barley shoots and bush bean leaves and stems was increased considerably in the presence of DTPA, however. In the absence of DTPA, the effect of added Pb was very small in the control and S amended soil treatments and almost negligible in the CaCO3 and MgCO3 amended soil treatments. Application of DTPA facilitated the translocation of Pb, Fe, Mn, Cu, and Zn to shoots. The effect was dependent upon soil pH. Particularly, the Fe was increased by DTPA at low pH while the effect was negligible at high pH. This was opposite the effect on Pb. The DTPA resulted in considerable Pb transport to leaves and stems at high soil pH. The uptake pattern of Zn and Cu was similar to that of Pb. It can be expected that chelating agents can increase the migration of Pb to plants andincrease its uptake by plants, and hence, entry into food chains.  相似文献   

8.
Influences of phosphorus and nitrogen on uranium and arsenic accumulation in Lemna gibba L. were investigated in the laboratory hydroponic cultures and in the field pot experiments. The initial uranium and arsenic concentrations in solutions for the hydroponic cultures were 1000 μ g l?1 each, while in situ trials used tailing water containing 198.7 ± 20.0 μ g U l?1 and 75.0 ± 0.4 μ g As l?1 at a former uranium mine in eastern Germany. A test of three PO4 3? concentrations (0.01, 13.6 and 40.0 mg l?1) in the hydroponic cultures, highest uranium accumulated in L. gibba under the culture with highest PO4 3?. Significant differences in uranium accumulation were between 0.01 mg l?1 and 13.6 mg l?1 PO4 3? cultures only (ANOVA p = 0.05). In the field, addition of 40.0 mg l?1 PO4 3? increased the bioaccumulation of uranium significantly. Contrary, high PO4 3? concentrations suppressed the bioaccumulation of arsenic in both the laboratory and the field. The bioaccumulation of both uranium and arsenic increased slightly with the increase of NH4 + concentration. However, high NH4 + concentrations reduced the yield in the control experiments. The concentration of uranium rose temporarily to 856.0 ± 294.0 μ g l?1, while the concentration of arsenic sunk slightly and temporarily immediately after amending the tailing waters with 40 mg l?1 PO4 3?. The speciation of uranium in the tailing water was modelled with geochemical code PhreeqC, which predicted that uranyl carbonate species dominated before addition of phosphates, but after increasing the PO4 3? concentrations, uranyl phosphates species became dominant. Addition of NH4 + to the tailing water had negligible influence on free available uranium and arsenic concentrations. Thus, manipulations to enhance uranium and arsenic attenuation by L. gibba has limitation when the amendments interact with other elements including the contaminants in the milieu, and when the target contaminants have antagonistic behaviour in the tailing water.  相似文献   

9.
Interactions between Zn and Cd on the accumulation of these metals in coontail, Ceratophyllum demersum were studied at different metal concentrations. Plants were grown in nutrient solution containing Cd (0.05–0.25 mg l?1) and Zn (0.5–5 mgl?1). High concentrations of Zn caused a significant decrease in Cd accumulation. In general, adding Cd solution decreased Zn accumulation in C. demersum except at the lowest concentration of Zn in which the Zn accumulation was similar to that without Cd. C. demersum could accumulate high concentrations of both Cd and Zn. The influence of humic acid (HA) on Cd and Zn accumulation was also studied. HA had a significant effect on Zn accumulation in plants. 2 mg l?1 of HA reduced Zn accumulation at 1 mg l?1 level (from 2,167 to 803 mg kg?1). Cd uptake by plant tissue, toxicity symptoms and accumulation at 0.25 and 0.5 mg l?1, were reduced (from 515 to 154 mg kg?1 and from 816 to 305 mg kg?1, respectively) by addition of 2 mg l?1 of HA. Cd uptake reached a maximum on day 9 of treatment, while that of Zn was observed on day 15. Long-term accumulation study revealed that HA reduced toxicity and accumulation of heavy metals.  相似文献   

10.

Purpose

This study delineated the effect of calcium (Ca) and ethylenediaminetetraacetic acid (EDTA) addition on arsenic (As) accumulation and physiological attributes of Vicia faba L.

Materials and methods

Two separate experiments were performed. In the first experiment, V. faba seedlings, grown under hydroponic conditions, were exposed to three levels of As (25, 125 and 250 μM) in the presence and absence of three levels of EDTA (25, 125, 250 μM) and calcium (CaCl2: 1, 5 and 10 mM). The effect of EDTA and Ca on As accumulation and physiological attributes of V. faba was assessed by determining As contents in roots and shoot, chlorophyll contents, H2O2 contents, and lipid peroxidation in young and old leaves. In the second experiment, V. faba seeds were grown in As-contaminated sand culture using the same treatment plan.

Results and discussion

The accumulation and toxicity of As to V. faba plants increased with increasing As levels in nutrient solution. Arsenic exposure enhanced the production of reactive oxygen species (ROS) in both roots and leaves, which resulted in lipid peroxidation and decreased chlorophyll contents. The presence of both EDTA and Ca, in general, significantly decreased As accumulation by V. faba seedlings, Ca being more effective than EDTA. Both the amendments decreased As-induced reactive oxygen species (ROS) production and lipid peroxidation. In the case of chlorophyll contents, EDTA significantly decreased chlorophyll contents, while Ca significantly increased chlorophyll contents compared to As. The effect of all the treatments was more pronounced in roots than leaves and in young leaves compared to old leaves.

Conclusions

It is proposed that EDTA and Ca greatly affect As accumulation and toxicity to V. faba, and the effect varies greatly with their applied levels as well as type and age of plant organs. The germinating seedlings of V. faba may be preferred for risk assessment studies, while transplanting 1-week-old V. faba seedlings to As-contaminated soils can decrease its toxicity.
  相似文献   

11.
Lygeum spartum, Zygophyllum fabago and Piptatherum miliaceum are typical plant species that grow in mine tailings in semiarid Mediterranean areas. The aim of this work was to investigate metal uptake of these species growing on neutral mine tailings under controlled conditions and their response to fertilizer additions. A neutral mine tailing (pH of soil solution of 7.1–7.2) with high total metal concentrations (9,100 and 5,200 mg kg?1 Zn and Pb, respectively) from Southern Spain was used. Soluble Zn and Pb were low (0.5 and <0.1 mg l?1, respectively) but the major cations and anions reached relatively high levels (e.g. 2,600 and 1,400 mg l?1 Cl and Na). Fertilization caused a significant increase of the plant weight for the three species and decreased metal accumulation with the exception of Cd. Roots accumulated much higher metal concentrations for the three plants than shoots, except Cd in L. spartum. Shoot concentrations for the three plants were 3–14 mg kg?1 Cd, 150–300 mg kg?1 Zn, 4–11 mg kg?1 Cu, and 1–10 mg kg?1 As, and 6–110 mg kg?1 Pb. The results indicate that neutral pH mine tailings present a suitable substrate for establishment of these native plants species and fertilizer favors this establishment. Metal accumulation in plants is relatively low despite high total soil concentrations.  相似文献   

12.
Acute toxicity screening tests were conducted with water soluble fractions (WSFs) of a solvent refined coal (SRC-II) liquid from a pilot plant and three reference organisms: the cladoceran Daphnia magna, the fathead (FH) minnow Pimephales promelas, and larvae of the midge fly Chironomus tentans. Stock WSFs typically contained 900 to 1100 mg l?1 total carbon (TC) and 700 to 800 mg l?1 total dye complexable phenolics, with lower concentrations of aromatic and saturate hydrocarbons and N compounds. Under standard test conditions (temperature 20 °C, pH 7.3 to 8.2 and hardness 65 to 80 mg l?1 CaCO3), mean LC50 values in mg l? TC were 3.3 for daphnia, 11.l for FH minnow, and 13.7 for midge larvae. Acute toxicity was also examined under other water quality conditions (temperature 10 or 25 °C, pH 6.0 or 6.5, and hardness ? 180 to 220 mg 1?1 CaCO3). The coal liquid was less toxic to daphnids at 10 °C than at 20 or 25 °C, but response of other organisms at different temperatures varied. The pH of the liquid had little effect on toxicity values. All organisms were less susceptible in hard water. Chemical compositions of stock WSFs were similar, suggesting that temperature, pH, and hardness had little effect on solubility of major synfucl components. Dilution indexes for stock WSFs were higher than for petroleum oils, and reflect the greater solubility of chemicals from the liquified coal in freshwater.  相似文献   

13.
This is a study of trace metal competition in the complexation of Pb(II) by well-characterized humic substances, namely Suwannee River Fulvic Acid (SRFA) in model solutions. It was found that Cu(II) seems to compete with Pb(II) for strong binding sites of SRFA when present at the same concentration as Pb(II). However, Cd(II) and Zn(II) did not seem to compete with Pb(II) for strong binding sites of SRFA. These two metals did compete with Pb(II) for the weaker binding sites of SRFA. Heterogeneity of SRFA was found to play a crucial role in metal–SRFA interactions. The environmental significance of this research for freshwater is that even at relatively low Pb(II) loadings, the metals associated with lead in minerals, e.g. Cu(II), may successfully compete with Pb(II) for the same binding sites of the naturally occurring organic complexants, with the result that some of the Pb(II) may exist as free Pb2+ ions, which has been reported to be one of the toxic forms of Pb in aquatic environment.  相似文献   

14.
Tamarix smyrnensis plants were exposed to Pb(NO3)2, with and without addition of salt into soil for 10 weeks. Salt as NaCl was added to water for watering in concentrations 0.5% and 3%. Subsequently, lead uptake was quantified in leaves and roots of the plants by ICP-AES spectrometry. In addition, the influence of Pb on photosynthesis and other physiological parameters was also examined by means of biomass production and shoot length determination. Plant appearance was observed also. The influence of Pb presence in the soil on chlorophyll content was examined. In order to quantify the fate of Pb in the plant, metal excretion by salt glands was investigated as well. It was found that the roots were the main accumulation site of Pb in all plants under all treatments irrespective of salt concentration in soil. Low salinity in soil did not affect Pb accumulation in roots but at high salt concentration Pb accumulation decreased. The opposite was observed in the leaves where more Pb was accumulated at high salt concentration in soil. The toxic effect of Pb was visible only in the group treated with Pb without addition of NaCl into water for watering. The visible toxicity symptoms were connected only with high salinity. No excretion of Pb by salt glands was observed.  相似文献   

15.
Numerous reports on boron nutrition of plants have been published since WARINGTON (15), using Vicia faba, first discovered in 1923 that boron is essential for the growth of higher plants. Inorganic (7, 9, 11) and organic (1) constituents of the growth medium and climatic conditions such as light intensity (13), photoperiod (5, 16), and drying and wetting of the soil (10) are some of the factors affecting boron uptake by plants.  相似文献   

16.
The effects of inoculating arbuscular mycorrhizal (AM) fungi on the growth, phosphorus (P) uptake, and yield of Welsh onion (Allium fistulosum L.) were examined under the non-sterile field condition. Welsh onion was inoculated with the AM fungus, Glomus R-10, and grown in a glasshouse for 58?days. Non-inoculated plants were grown as control. Inoculated and non-inoculated seedlings were transplanted to a field with four available soil P levels (300, 600, 1,000, and 1,500?mg P2O5?kg?1 soil) and grown for 109?days. AM fungus colonization, shoot P concentration, shoot dry weight, shoot length, and leaf sheath diameter were measured. Percentage AM fungus colonization of inoculated plants was 94% at transplant and ranged from 60% to 77% at harvest. Meanwhile, non-inoculated plants were colonized by indigenous AM fungi. Shoot length and leaf sheath diameter of inoculated plants were larger than those of non-inoculated plants grown in soil containing 300 and 600?mg P2O5?kg?1 soil. Shoot P content of inoculated plants was higher than that of non-inoculated plants grown in soil containing 300 and 600?mg P2O5?kg?1 soil. Yield (shoot dry weight) was higher for non-inoculated plants grown in soil containing 1,000 and 1,500?mg P2O5?kg?1 soil than for those grown in soil containing 300 and 600?mg?P2O5 kg?1 soil. Meanwhile, the yields of inoculated plants (200?g plant?1) grown in soils containing the four P levels were not significantly different. Yield of inoculated plants grown in soil containing 300?mg P2O5 kg?1 soil was similar to that of non-inoculated plants grown in soil containing 1,000?mg P2O5?kg?1 soil. The cost of AM fungal inoculum for inoculated plants was US$ 2,285?ha?1 and lower than the cost of superphosphate (US$ 5,659?ha?1) added to soil containing 1,000?mg P2O5 kg?1 soil for non-inoculated plants. These results indicate that the inoculation of AM fungi can achieve marketable yield of A. fistulosum under the field condition with reduced application of P fertilizer.  相似文献   

17.
Uptake of barium (Ba) from soil by vascular plants varies among species. Despite the toxicity of soluble Ba compounds to plants, research on mechanisms controlling Ba uptake from natural soils is scarce. This study investigated the treatment effect of lime (CaCO3) added to a sandy soil containing a total of 500 mg Ba kg?1 on uptake and interspecies distribution of Ba in legumes and other cultivated food and feed plants. Nine species of grasses, vegetables, herbs, and legumes were cultivated under controlled conditions in a greenhouse experiment. The plants were harvested at maturity or flowering, dried, milled, and digested with nitric acid using the microwave technique prior to ICP‐MS analysis. All plant species acquired Ba from the soil in considerable amounts, probably due to low Ba adsorption potential of the sandy soil. Shoot tissue concentrations ranged from about 100 (grass) to 600 mg Ba kg?1 (legume) and root concentrations from about 100 (tuber vegetable) to 700 mg kg?1 (legume). Vicia cracca L. (bird vetch) showed an accumulation capacity due to high shoot concentrations of Ba compared to the other species. Higher yield accompanied by a potential dilution effect can partly explain why Trifolium repens L. (white clover), Pisum sativum ssp. arvénse L. (gray pea) and Hordeum vulgare L. (barley) did not display the highest Ba concentrations, but showed the highest Ba uptake from soil. High plant uptake of calcium (Ca) also seemed to enhance Ba uptake, by legumes in particular. However, liming reduced shoot Ba concentrations, particularly of species with low affinity for Ca. The risk of Ba accumulation thus raises toxicity concerns when forage legumes are cultivated in soils containing elevated concentrations of Ba.  相似文献   

18.
After the toxic spill occurred at Aznalcóllar pyrite mine (Southern Spain), a wide area of croplands near the Doñana Wild Park was contaminated with 4.5 million m3 of slurries composed of acidic waters loaded with toxic metals and metalloids such as As, Sb, Zn, Pb, Cu, Co, Tl, Bi, Cd, Ag, Hg and Se. Today, 6 years after the spill, the concentration of toxic elements in these soils is still very high, in spite of the efforts to clean the zone. However, some plant species have colonised this contaminated area. Legumes possessing N2-fixing nodules on their roots represented a significant proportion of these plants. Our objective was to use the Rhizobium-legume symbiosis as a new tool for bioremediate the affected area. We have isolated about 100 Rhizobium strains, 41 of them being resistant to high concentrations of As (300 mg l−1), Cu (100 mg l−1) and Pb (500 mg l−1). Their phenotypes and bioaccumulation potentials have been characterised by their growth rates in media supplemented with As and heavy metals. The presence of the resistance genes in some strains has been confirmed by PCR and Southern blot hybridisation. Several Rhizobium were symbiotically effective in the contaminated soils. On the other hand, the first steps in nodule establishment seemed to be more affected by heavy metals than N2-fixation.  相似文献   

19.
The phytoremediation potential for Pb of Buddleja asiatica (a wild species) and a closely related cultivated species, B. paniculata, was investigated by means of field survey, hydroponic and pot experiments, and field trial experiments. Field surveys showed that B. asiatica had an extraordinary accumulation capacity and tolerance for Pb. Plants grown in soil with 2,369.8–206,152 mg kg?1 total Pb accumulated 1,835.5–4,335.8 mg kg?1 Pb in their shoots. Under hydroponic conditions (10, 20 mg l?1 Pb), both B. asiatica and B. paniculata showed unusually high concentrations of Pb in their roots (12,133–21,667 mg kg?1) and increased biomass production. A pot experiment in a greenhouse without any soil amendments was conducted on three different soils with various Pb contents (10,652, 31,304, 89,083 mg kg?1) for 3 months. The results showed that both species of Buddleja had an increase in the biomass similar to the control plants. There was a slight decrease in survival rates of plants grown in soil with 89,083 mg kg?1 Pb content. A field trial experiment was conducted for 6 months at three sites around the Pb mine area in which plants were provided with Osmocote fertilizer. Both Buddleja species showed 100% survival, increased biomass production and phytoextraction capacity (TF 1.1–2.3) when grown in soil with Pb content of 94,584–101,405 mg kg?1. Plants accumulated 2,273–3,675 mg kg?1 Pb in their shoots. The results suggest these Buddleja plants are suitable for use in the phytoremediation of Pb-contaminated soil.  相似文献   

20.
《Applied soil ecology》2007,35(1):163-173
Two pot experiments were conducted to investigate the effect of inoculation with the arbuscular mycorrhizal (AM) fungus Glomus intraradices on Pb uptake by two clones of Nicotiana tabacum plants. Non-transgenic tobacco plants, variety Wisconsin 38, were compared in terms of Pb uptake with transgenic plants of the same variety with inserted gene coding for polyhistidine anchor in fusion with yeast metallothionein. Bioavailability of Pb in experimentally contaminated soil was enhanced by the application of a biodegradable chelate ethylenediaminedissuccinate (EDDS).EDDS addition (2.5 and 5.0 mmol kg−1 substrate) increased Pb uptake from the substrate and enhanced Pb translocation from the roots to the shoots, with shoot Pb concentrations reaching up to 800 mg kg−1 at the higher chelate dose. Application of a single dose of 5 mmol kg−1 proved to be more efficient at increasing shoot Pb concentrations than two successive doses of 2.5 mmol kg−1, in spite of a marked negative effect on plant growth and phytotoxicity symptoms. Pb amendment (1.4 g kg−1 substrate) connected with either dose of EDDS decreased significantly plant biomass as well as reduced the development of AM fungi. AM inoculation promoted the growth of tobacco plants and partly alleviated the negative effect of Pb contamination, mainly in the case of root biomass.No consistent difference in Pb uptake was found between transgenic and non-transgenic tobacco plants. The effect of AM inoculation on Pb concentrations in plant biomass varied between experiments, with no effect observed in the first experiment and significantly higher root Pb concentrations and increased root–shoot ratio of Pb concentrations in the biomass of inoculated plants in the second experiment. Due to probable retention of Pb in fungal mycelium, the potential of AM for phytoremediation resides rather in Pb stabilisation than in phytoextraction.  相似文献   

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