共查询到19条相似文献，搜索用时 656 毫秒
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《华北农学报》2016,(3)
芸芥与芸薹属植物具有亲缘关系,也是芸薹属植物的重要育种资源。自交亲和性是芸芥的一种重要变异性状。为了探明芸芥自交亲和基因的表达器官,并分离与自交亲和性有关的cDNA片段,以芸芥的一对近等基因系,自交亲和系(SC)与自交不亲和系(SI)为试材,利用差异显示RT-PCR技术分别对开花前和开花后的叶片、花药和柱头进行鉴定。结果表明,不论开花前还是开花后,芸芥自交亲和系与自交不亲和系的叶片或花药间的扩增带型是一样的,但在近等基因系的柱头间得到了差异表达条带。这证明芸芥自交亲和基因不是组成型表达,而是组织特异性表达,柱头是其唯一的表达器官。在开花前的自交亲和系中共得到了2条cDNA片段,一条小于100 bp,另一条为750~1 000 bp,在开花后的自交亲和系中得到了1条300 bp的cDNA片段。据分析这3条cDNA片段可能与芸芥的自交亲和性密切相关。在开花前的自交不亲和系中共得到了2条cDNA片段,一条为500 bp,另一条为750 bp,同时在开花后的自交不亲和系中得到了1条500~600 bp的cDNA片段。这些cDNA片段可以用来区分芸芥的自交亲和系与自交不亲和系,还可用于克隆自交亲和基因。 相似文献
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《华北农学报》2015,(6)
为研究芸芥自交亲和机理,采用mRNA差异显示反转录PCR(DDRT-PCR)分析技术,从芸芥自交亲和系开花前柱头中获得1条差异片段。测序及DNA序列的生物信息学分析表明,该差异片段与拟南芥DnaJ蛋白同源基因At J3有较高同源性,暂命名为esAtJ3基因。该序列含有一个长度为297 bp的完整开放阅读框,编码98个氨基酸残基,含有一个DnaJ-C保守结构域,属于植物DnaJ超家族。esAtJ3基因编码的蛋白无信号肽,是一个亲水性蛋白,该蛋白主要由不规则卷曲和α螺旋组成。实时荧光定量PCR分析表明,esAtJ3基因在芸芥SC系开花前柱头中表达量较高,初步推测该基因可能参与芸芥亲和性调控。 相似文献
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由于目前国内外甘蓝杂交种绝大多数均是利用自交不亲和系杂交而得,自交不亲和系留种十分困难,主要靠人工蕾期授粉,费工费时、成本高,且自交多代易导致生活力衰退、种子产量低、籽粒不饱满等,向来是制约甘蓝育种和大面积制种的问题。因此要提高甘蓝杂交种制种产量,必先进行甘蓝自交不亲和系繁种技术研究。本课题组历经3年试验摸萦,以自育品种黔甘系列甘蓝自交不亲和系a2-13、g47-4、E4-2等为试材,通过进行不同处理对克服甘蓝自交不亲和性的效果试验、根外追肥对提高甘蓝自交不亲和系结实效果试验、不同授粉方式对甘蓝自交不亲和系种子产量和… 相似文献
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综述了近20年日本梨中与自交亲和和自交不亲和有关的S4基因的研究进展。这些结果包括：“二十世纪”(基因型为S2S4)为自交不亲和品种,其突变品种“奥嗄二十世纪”(基因型 为S2S4SM,SM=stylar part mutant;花柱部分突变)为自交亲和型。“奥嗄二十世纪”自交亲和的原因有三点：(1)S4SM基因被删除或者是低水平表达。(2)S4SM基因仅在柱头表达。(3)S4SM基因表达较迟。这主要是由于“二十世纪”S基因座上的S4等位基因发生了高度突变成为S4SM基因或者S4等位基因被删除,导致其基因产物花柱中的S4-RNase受到高度抑制或缺乏的缘故。S4基因由997bp组成,其中85~768bp为684bp的阅读框,925~931bp和943~950bp为多聚腺苷酸信号。S4基因的表达产物为S4蛋白,S4蛋白由228个氨基酸残基组成,其中N端的27个氨基酸残基为信号肽,成熟的S4蛋白由201个氨基酸残基组成。S4蛋白的生理功能是抑制花粉的萌发和花粉管的伸长。 相似文献
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旨在克隆获得新疆野扁桃自交不亲和花粉SFB新基因,以新疆野扁桃花药为试材,应用分子同源克隆及RT-PCR等技术,并进一步采用在线软件分析SFB基因的蛋白质理化性质,实验成功克隆到了PtSFB5与PtSFB6 2个新SFB基因的cDNA全长,2个基因的cDNA全长分别为1124 bp和1072 bp,分别编码了374和348个氨基酸,2个基因都具有F-Box基因结构,属于F-Box基因家族,预测的相对分子量分别为30787.26和44037.74,等电点为5.93和5.43,均属于水溶性不稳定蛋白。 相似文献
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大白菜自交不亲和系自交不亲和性的克服山东省种子管理总站（济南２５０１００）王桂娥自交不亲和系植株花期自交及花期系内姊妹交都不易授粉结实，因此，要保质保量繁好大白菜自交不亲和系原种，必须克服其自交不亲和性。１蕾期授粉法大白菜自交不亲和系在花期虽然不能自... 相似文献
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蔬菜杂种优势的利用,其关键是要解决制种的技术问题,由于在去雄、授粉中所需劳力大,杂种F1的种子生产成本高,其应用一度受到限制.近年来,随着雄性不育系和自交不亲和系的选育成功,其使用范围得到进一步的扩大,特别是十字花科蔬菜自交不亲和系的应用发展迅速. 相似文献
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Summary Self-incompatibility was shown to be an effective method of pollination control in spring rapeseed (B. napus L. ssp. oleifera (Metzg.)) by comparing the yield of a Westar-Topas syn-1 produced by crossing two SI lines with the yield of the corresponding syn-1 produced by hand pollination. Although the trial showed high-parent heterosis in the syn-1s, there was insufficient replication to determine the level of heterosis.Abbreviations SI
self-incompatible
- SC
self-compatible 相似文献
12.
A self-compatible (SC) variant of a wild diploid potato species, Solanum chacoense, which is normally self-incompatible (SI),
was investigated for the nature and genetics of self-compatibility. It was crossed with a SI cultivated diploid potato species,
S. phureja. The F1 progeny segregated SC vs. SI. Diallel crosses were made among 15 F1's. Self-compatibility was tested in a selfed family of a parental SC variant and in sib-mated and selfed families of F1 progeny. All the data suggest that there is a single dominant gene (Sli) with sporophytic action inhibiting S gene expression
in the pollen. Plants having a ‘Sli’ gene, produce pollen which is compatible to its own parent and plants with similar S
genes. The ‘Sli’ gene has been maintained in a heterozygous condition through eight selfing generations (S8) implying that dominant homozygotes might be associated with lethality.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
13.
Resynthesis of Brassica napus L. for self-incompatibility: self-incompatibility reaction, inheritance and breeding potential 总被引：3，自引：0，他引：3
M. H. Rahman 《Plant Breeding》2005,124(1):13-19
Self-incompatibility (SI) in Brassica has been considered as a pollination control mechanism for commercial hybrid seed production, and so far has been extensively used in vegetable types of Brassicas. Oilseed rape Brassica napus (AACC) is naturally self-compatible in contrast to its parental species that are generally self-incompatible. Introduction of S-alleles from its parental species into oilseed rape is therefore needed to use this pollination control mechanism in commercial hybrid seed production. Self-incompatible lines of B. napus , carrying SI alleles in both A and C genomes, were resynthesized from self-incompatible B. oleracea var. italica (CC) cv.'Green Duke' and self-incompatible B. rapa ssp. oleifera (AA) cv. 'Horizon', 'Colt' and 'AC Parkland'. All resynthesized B. napus lines exhibited strong dominant SI phenotype. Reciprocal cross-compatibility was found between some of these self-incompatible lines. The inheritance of S-alleles in these resynthesized B. napus was digenic confirming that each of the parental genomes contributed one S-locus in the resynthesized B. napus lines. However, the presence of two S-loci in the two genomes was found not to be essential for imparting a strong SI phenotype. Possible use of these dominant self-incompatible resynthesized B. napus lines in hybrid breeding is discussed. 相似文献
14.
A. H. Eenink 《Euphytica》1981,30(1):71-76
Summary For the production of inbred lines and F1 hybrids in witloof-chicory information is wanted on characteristics such as the incompatibility system. These characteristics can only be studied properly if the influence of temperature and physiological status of the plant on pollen germination and seed production is known. Investigations were carried out with 9 self-incompatible (SI) and 6 self-compatible (SC) clones in glasshouses of the IVT phytotron at constant temperatures of 10, 14, 17, 20, 23 and 26°C. In general, in vivo pollen germination percentages were rather low after self pollination with an optimum for germination around 17–20°C. No seeds were formed at the lowest temperature (10°C) while seed production for SC clones was usually (rather) good at higher temperatures. At 26°C seed production in some clones decreased. Both pollen germination and seed production decreased at the end of the flowering period. There was a rather positive relationship at e.g. 17 and 20°C between pollen germination after selfing and seed production. When no pollen germination was observed, no seed formation occurred. When pollen grains did germinate, seed development would not necessarily occur in all cases. So this relationship only enables negative mass selection for SC. 相似文献
15.
Summary
Brassica napus is a natural allotetraploid derived from the diploid species B. rapa L. (syn. campestris L.) and B. oleracea L. Somatic hybrids synthesized from highly heterozygous lines of these two diploid species were evaluated for fertility. The hybrids were obtained from two fusion experiments which differed in the B. rapa full-sibling parent used as the source of protoplasts. Both B. rapa siblings were lelf-incompatible (SI) yet contained different S-alleles; the B. oleracea species parent was self-compatible (SC). Eight tetraploid hybrids examined had very high female and male fertility; eight hybrids with higher ploidy had low fertility. Hybrids derived from one B. rapa sibling were self-incompatible, whereas those derived from the other B. rapa sibling were fully self-compatible. These data suggest that the different S-alleles of each B. rapa sibling displayed varying penetrance relative to the SC of the B. oleracea parent when combined in B. napus.Abbreviations SC
self-compatibility
- SI
self-incompatibility 相似文献
16.
A self-compatible (SC) hybrid plant F1-1 was obtained from a cross between a SC variant of a wild diploid potato species, Solanum chacoense, and a self-incompatible
(SI) cultivated diploid species, S. phureja. The clone F1-1 has previously been proposed to have a dominant S locus inhibitor gene (Sli) in a heterozygous condition. It was crossed
as a male parent with a selected clone from a S. stenotomum-S. phureja population, resulting in a segregating population consisting
of 116 hybrid plants. Self-compatibility was assessed by selfing each of the hybrids. Sixty-six of them were SC, while 35
were SI, showing a significant distortion from an expected Mendelian ratio of 1:1. A genetic linkage map was constructed using
DNA markers to localize the Sli gene. A total of 28 RAPD and 127 RFLP markers identified 109 mapping positions on 12 linkage
groups. The Sli gene was mapped at a distal end of chromosome 12. Since the S locus has been localized on chromosome 1 on
the potato RFLP map, it is confirmed that the Sli gene is independent of the S locus.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
17.
用mRNA差别显示方法分析黑麦盐胁迫下应答基因cDNA片段的表达特性 总被引：15，自引：0，他引：15
利用mRNA差别显示方法分析经250mmol/LNaCl处理3d的黑麦幼苗,14个差异cDNA片段,包括8个诱导表达片段,2个增强表达片段和4个抑制表达片段被检测到.NorthernBlot分析证实,其中0.8kb、0.65kb和0.35kbcDNA片段有强烈阳性信号,命名为SI800、SI650、SI350(SaltInduced800bp、650bp、350bp),证明与盐胁迫有关.以SI800为探针与分别经 相似文献
18.
Identification and genetic characterization of different resistance sources to ascochyta blight within the genus Lens 总被引：2，自引：0，他引：2
The self-incompatibility mechanism in 10 local radish cultivars was investigated through seed set analysis and pollen tube
growth behavior. The objective was to identify self-incompatible local radish lines for hybrid seed production. Based on a
seed set ratio as an estimate for self-incompatibility, cultivars Tangail Local, Tasaki, Kuni, Aushi and Indian Aushi were
identified as self-compatible. An intermediate seed ratio was observed in ‘Red Mollika’, ‘Pinky’ and ‘Red Bongi’. Moderately
strong self-incompatibility was observed in ‘Red Kalpin’ and ‘Kuni White’. Pollen tube growth behavior revealed that self-incompatible
lines had a relatively lower number of germinated pollen grains per stigma and pollen tubes per style than those of self-compatible
lines. The two self-incompatibility test methods gave comparable results. None of the cultivars under study was found to be
fully self-incompatible.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
19.
胡萝卜atp8基因的克隆表达及细胞质雄性不育的相关性研究 总被引：1，自引：1，他引：0
摘 要：线粒体基因重组而形成的异常开放阅读框的表达导致了植物的细胞质雄性不育现象,在这种现象的研究中具有重要的意义。本研究分离克隆了胡萝卜细胞质雄性不育相关的线粒体基因片段,并利用半定量RT-PCR和Real-time PCR技术研究了该基因在不育系和相应保持系中的表达情况。结果表明,与不育系相比,胡萝卜保持系atp8基因序列在30 bp-170 bp之间核苷酸突变很多,且在154 bp-162 bp发生了缺失。Blast分析发现保持系atp8基因与胡萝卜nad6基因的同源性高达100%。表达分析表明,atp8基因在不育系花蕾的前五个时期表达量都明显低于相应的保持系,而在盛花期的表达量与保持系趋于一致。推测atp8基因的异常表达与胡萝卜细胞质雄性不育有着密切关系。 相似文献