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1.
The use of hair as a sample matrix to determine the mineral status of an animal has received a lot of interest. The objective of this study was to determine if the trace element content in horse mane hair changed with age when evaluated in a group of horses representing a large age range. As a second objective, whole blood trace element content was evaluated, and its relationship to mane hair trace element content, as well as age, were tested. Therefore, mane hair and whole blood samples were obtained from 59 horses, ranging from 2 months to 26 years in age, housed on the same farm. Mane hair samples were washed, and hair and blood digested and analyzed for 11 trace elements. Weak correlations (P < .05) between age and mane hair trace element content was detected for arsenic (r = 0.29), copper (r = −0.39) and selenium (r = −0.27). While the mane hair color did not affect trace element concentration, greater variability was detected in the black mane hair samples. This resulted in outliers that were removed from the final statistical analysis. However, data is presented to the reader both ways. Correlations were also detected (P < .05) between age and whole blood iron (r = 0.62), selenium (r = 0.76) and zinc (r = 0.47). This is similar to what has been reported in horses using serum or plasma. The trace element concentrations of mane hair and whole blood were not correlated (P > .05) in this study. However, the concentrations of trace elements in mane hair were higher than that of blood. Trace elements, specifically chromium and lead, that were below detection levels in the blood, were detectable in mane hair. This suggests that mane hair may be a potential means to investigate suspected exposure to excessive levels of trace minerals or heavy metals that are difficult to detect in blood.  相似文献   

2.
The beta-agonist clenbuterol in mane and tail hair of horses   总被引:2,自引:0,他引:2  
REASONS FOR PERFORMING STUDY: The beta2-agonist clenbuterol is commonly administered for therapeutic purposes in the horse, but its use an an anabolic agent is illegal. Clenbuterol can be detected in blood and urine for a relatively short period after administration and detection in hair could enhance the analytical range and be used to determine the history of clenbuterol application. HYPOTHESIS: That detection in mane or tail hair is possible over an extended period. METHODS: Four horses received 0.8 microg clenbuterol hydrochloride/kg bwt b.i.d. for 10 days. Four other horses were used as untreated controls. Blood, urine, mane and tail hair samples were taken on Day 0 (before) and 5, 10, 30, 35, 40, 60, 90, 120, 150 and 360 days after start of treatment. Gas chromotography/high resolution mass spectrometry (GC/HRMS) was developed for clenbuterol analysis: limit of detection was 0.2 pg/mg; intra-assay repeatability limit r = 0.06 (confidence level 95%); interassay repeatability limit r = 0.03 (confidence level 95%). Prior to treatment, clenbuterol was absent from all samples analysed. RESULTS: Clenbuterol was detectable as early as Day 5 in tail and mane hair of Segment 1 (0-20 mm from the roots) and was maximal on Day 90. However, as time progressed, shift into lower 20 mm segments was observed. On Day 360, the maximum concentration (up to 21 pg/mg) was located in Segment 13, i.e. 26-28 cm from roots of hair. Clenbuterol was not detectable in blood or urine after Day 30. Mane and tail hair results were very similar. CONCLUSIONS: The study showed that the beta-agonist clenbuterol can be found in mane and tail hair of horses after extended periods. POTENTIAL RELEVANCE: It will be possible to detect clenbuterol in breeding and show horses where anabolic drugs have been used illegally to improve conformation. This method may also be helpful to monitor therapeutic clenbuterol treatment.  相似文献   

3.
To investigate the relationships between trace elements concentrations in hair and atrial fibrillation (AF) in horses, concentrations of nineteen trace elements were detected in hair using the particle induced X-ray emission (PIXE) method. The horses were assigned to either control (n=22, no abnormalities) or AF groups (n=5) based on electrocardiograph findings. The mean concentrations of Ca and Zn in the hair of the AF group were significantly higher than those in the control group. The Zn/Cu ratio of the hair in the AF group (29.8 +/- 5.5) was significantly higher than that in the control group (23.4 +/- 2.2, P<0.05). The results of the present study suggest that there is a relationship between elevated concentrations of Ca and Zn in hair and AF.  相似文献   

4.
研究产奶量对热应激和非热应激期间奶牛血液、乳、和毛中微量元素的影响。结果表明,①测到全血中7种微量元素中,在非热应激期间,产奶量为10~20 kg时,奶牛血液中Zn、Mn的含量明显增加(P<0.05),Fe的含量减少,差异显著(P<0.05)。热应激期间,Fe、Cu的含量随产奶量增加逐渐减少,Zn含量增加,其它元素的含量无规律性变化。在热应激期,随奶牛的产奶量的增加,排泄到乳中的Zn也明显增加。非热应激反应的牛,随着产奶量的增加,而排泄到乳中的Cr明显增加(P<0.05),而热应激反应牛,Cr含量有明显下降的趋势(P<0.05),高产奶牛的乳中Se含量明显高于低产牛的(P<0.05),在产奶量为5-10 kg时排泄到乳中Mn的含量比其它产奶阶段有明显增加(P<0.05)。在非应激期,不同的产奶量对毛中Fe、Cr、Se有明显的影响(P<0.05)。对毛中的Zn、Co、Cu、Mn无明显影响。在热应激期,不同的产奶量对毛中Fe、Se、Co分布影响极明显(P<0.01)。  相似文献   

5.
从奥系西门塔尔牛群中选公母牛各3头,检测被毛、全血和乳中Fe、Cu、Co、Mn、Zn与Se的含量;还检测3种公牛(16头次)精液中Co、Mn、Zn和Se的含量。将测定值与日粮的微量元素含量进行综合分析看出,该牛群日粮中Mn含量明显不足,Cu、Zn略欠,Fe和Se足够。被毛中微量元素含量是衡量牛微量元素营养的较好指标。  相似文献   

6.
为明确甘肃陇东地区苜蓿草地土壤矿质养分亏缺及牧草养分水平,对环县、合水、庄浪、泾川的苜蓿和土壤的N、P、K、Fe、Mn、Cu、Zn含量及其相关性进行研究。结果表明:试验区土壤N含量略高于我国平均水平、P含量低于我国平均水平,K素略低于我国平均水平;土壤矿质元素含量为有效Mn极富、有效Zn中等、有效Fe、Cu缺乏。苜蓿中N含量适中,P、Fe、Mn、Cu、Zn含量较低,K素含量高;苜蓿对K素需求量高于所测其他矿质养分;苜蓿微量元含量由高到低为FeMnZnCu,且苜蓿对Fe的需求高于Mn,Zn和Cu。土壤与苜蓿的全P含量极显著相关(r=-0.738,P0.01),全N含量显著相关(r=-0.522,P0.05),有效Fe含量显著相关(r=0.61,P0.05),有效Zn含量呈高度相关(r=0.742,P0.05);土壤与苜蓿全K、有效Cu、有效Mn含量未呈现出显著关系。  相似文献   

7.
OBJECTIVE Compare the career profiles of a cohort of Standardbred horses that first raced as 2-year-olds with those that started their racing careers at a later age. METHOD Retrospective analysis of the racing records of all foals born in New South Wales in the 2000 foaling season. RESULTS The career records of 999 horses were analysed. Almost half (43.9%) first raced as 2-year-olds and one-third (33.9%) as 3-year-olds. The median career duration for horses that first raced as 2-year-olds was 2.93 years (interquartile range (IQR) 2.70-3.16), which was significantly greater than the median for horses that first raced at 3, 4 or ≥5 years old (P < 0.001). Males, and horses that first raced as 2-year-olds, earned significantly more prize money than females or horses that started racing aged ≥3 years (P < 0.001). The population median number of career starts was 28.0 (IQR 8-64). Males, and horses that first raced as 2-year-olds, had significantly more career starts than females or horses that started racing aged ≥3 years (P < 0.001). CONCLUSION This study found no evidence suggesting that racing as a 2-year-old had a deleterious effect on a horse's racing career.  相似文献   

8.
The objective of the study was to examine the career profile of the average racehorse in order to establish normal values for performance. Records of race performance for a population of 762 horses randomly selected from the 1972 registrations of the Canadian Standardbred Horse Society were summarized and the pattern of racing by year and month from 1974 to 1983 inclusive recorded. Results were analyzed to determine the influence of sex, gait, age, and year of first race (starting year). The transformation log (1 + X) was used to achieve normality where necessary. Of the 762 horses, 507 raced, 56 (11.0%) of which took one or more complete seasons (years) off. No age predisposition was detected, and no significant difference in mean career performance between the group taking a season off and the remaining horses were found, with the exception of career length, which was longer for those temporarily absent from racing (p less than 0.001). No influence upon career pattern for horses off early in their career was observed but those off late showed indications of a subsequent fall in number of races per year and in times for the mile. Mean career duration was 25.66 to 28.38 months (95% confidence limits for the mean, n = 507). Absence from racing of at least one months duration was taken by 437 horses, mean percentage of career off 42.36 +/- 0.94% (mean +/- SEM), mean duration of periods off 4.43 to 4.71 months. Career duration in months and percentage off were only weakly related (r = -0.16).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

9.
Detection of enrofloxacin and its metabolite ciprofloxacin in equine hair   总被引:4,自引:0,他引:4  
Hair analysis to detect drug administration has not been studied extensively in horses. This study aimed to (a) develop an analytical method for enrofloxacin and its metabolite ciprofloxacin in mane and tail hair, (b) relate measured values to doses, routes of administration, hair colour, and (c) demonstrate long-term detectability. Samples were extracted in trifluoroacetic acid at 70 degrees C. Extracts were cleaned-up by solid-phase extraction and analysed by high-performance liquid chromatography with UV-diode array detection. Analyte recoveries were > 87%. Horses were sampled after therapeutic enrofloxacin administration either orally at 7.5 mg/kg daily for 3-13 days or twice daily for 10-14 days (Group 1, n=7) or intravenously at 5.0 mg/kg daily for 12 and 15 days (Group 2, n=2). Enrofloxacin and ciprofloxacin were detected at concentrations up to 452 and 19 ng/mg, respectively, up to 10 months post-treatment. In vitro, enrofloxacin and ciprofloxacin were extensively bound to melanin (> 96%) and in vivo, their uptake was 40-fold greater in black than white hair. Enrofloxacin and ciprofloxacin concentrations correlated to enrofloxacin dose (r2=0.777 and r2=0.769). Enrofloxacin:ciprofloxacin ratios were 21:1 and 13:1 following intravenous and oral administration, respectively. Longitudinal analyte distributions correlated to treatment-sampling interval.  相似文献   

10.
本试验旨在分析金川县牦牛血清和被毛的矿物元素含量,探讨肋数和年龄对矿物元素含量的影响,并对被毛矿物元素含量与血清中相应元素含量的相关性进行分析。采用二因素有重复试验设计,将32只公牦牛进行分组,按照肋数[14肋(n=17)和15肋(n=15)]和年龄[0(初生)~2岁(n=10)、3~4岁(n=11)、5~6岁(n=5)、7~8岁(n=6)]共分为8组。分别采集血清及被毛样品,进行矿物元素含量分析。结果表明:1)肋数、年龄对血清中的钙(Ca)、铜(Cu)、铁(Fe)、钾(K)、镁(Mg)、锰(Mn)、钠(Na)、锌(Zn)的含量均无显著影响(P0.05);年龄和肋数间无互作(P0.05)。2)肋数对被毛Ca、Cu、Fe、Mg、Mn、Na、Zn含量无显著影响(P0.05);年龄可影响被毛K含量,7~8岁被毛K含量显著高于其他年龄段(P0.05),但对被毛其他元素含量均无显著影响(P0.05),年龄和肋数间无互作(P0.05)。3)被毛Ca、Cu、Fe、Mg、Zn与血清中相应元素含量相关性极显著(P0.01),相关系数分别为0.623 0、0.539 8、0.569 2、0.468 4、0.505 3,而K、M n、Na含量的相关性则不显著(P0.05)。综合可知,金川14肋与15肋牦牛矿物元素含量无显著差异;7~8岁被毛中的K含量显著升高;牦牛被毛、血清Ca、Cu、Fe、M g、Zn含量具有显著的相关性,可由被毛代替血清测定相应的矿物元素含量。  相似文献   

11.
Abstract

The genetic associations between racing performance and preselection of horses considered as the binary trait racing status (trotters without or with at least one racing performance in life were classified as 0 and 1, respectively) as well as disqualified races (disqualified and non-disqualified trotters were classified as 1 and 0, respectively) were analysed in German trotters. Variance components for racing performance traits square root of rank at finish, racing time per km, and log of earnings with racing status were estimated based on an animal model using REML. Heritabilities of racing status, racing time and rank at finish were 0.30, 0.21, and 0.06, respectively. The genetic correlations between racing status and racing time or rank at finish were ?0.74 and ?0.32, indicating that horses started at least once showed a higher genetic potential in racing time or finishing ability than never started horses. This showed the high preselection of German trotters especially based on racing time. To account for this preselection, it was recommended for additional use of racing status in the German evaluation system. Breeding values of the three racing performance traits were estimated by two distinct models, in- or excluding racing status and compared by using three criteria. Racing time per km showed the highest correlation (r=0.98) between breeding values evaluated by these two distinct models. Therefore, incorrect selection rate of horses using breeding values from the model without racing status, was lowest for racing time per km (9.7%). Selection response increased about 1% for this trait after including racing status in the model. For the estimation of rank at finish, inclusion of racing status in the multiple trait model was much more important as indicated by a low correlation between breeding values (r=0.29) and high percentage of incorrectly selected stallions (97.5%). The trait disqualified races was first analysed using an univariate threshold model. Heritability of this trait was low (h 2=0.12) and repeatability (r=0.43) showed a moderate magnitude. Using a linear multiple trait animal model, disqualified races showed a low heritability (h 2=0.05) and a moderate favourable genetic correlation (r g=0.43) with racing time per km. Consequently, selection on racing time per km is expected to improve indirectly the reliability of racing performance. Combined selection of reduction in disqualified races and racing time may even further improve the reliability of racing trotters.  相似文献   

12.
北京黑白花乳牛蹄底角质中25种元素含量的研究   总被引:3,自引:0,他引:3  
本实验采用电感耦合等离子体原子发射光谱仪,在1987年春、秋两季对北京某牛场的黑白花成年乳牛蹄底角质中25种元素(K、Na、S、Ca、P、Mg、Mn、Mo、Cu、Zn、Fe、I、Se、Co、Cd、Cr、Ni、B、Al、Si、Ti、Ba、Ph、As、Hg)进行了测定,并对春、秋季正形蹄与变形蹄、正形蹄与病变蹄,以及春秋正形蹄、变形蹄之间做了对比,还测定了饲料地的土壤、水和饲料中25种元素的含量,与国内外有关资料的结果进行了对比,初步探讨了某牛场成乳牛中正形蹄蹄底角质中25种元素的含量,指出春秋两季正形蹄蹄底角质中25种元素的含量是有差异的,春季正形蹄与变形蹄相比各元素差异均不显著;秋季变形蹄含Mg量显著高于正形蹄,含Ca、Al、Cd和Ti量显著低于正形蹄;春季病变蹄含K、Fe、Mn、Cu、Mg、Al、Ti、Se、I、Pb、Hg量显著高于正形蹄。本文首次提出北京黑白花乳牛正形蹄蹄底角质中Ca、P比值,春季是5.822/1,秋季是6.253/1。此牛场乳牛饮水中Hg含量过高是环境污染的结果。本文为今后进一步研究北京黑白花乳牛蹄角质与元素的关系提供了临床参考的依据。  相似文献   

13.
1. The study was part of a project designed to investigate if organic selenium (Se) can ameliorate the toxic effects of cadmium (Cd). The main objective of the present study was to investigate, in the chicken, the interactions between Se, Cd and the following elements: Sb, Ca, Cr, Co, Cu, Fe, Pb, Mg, Mn, Mo, Ni, V and Zn.

2. A total of 300 1-d-old chickens (broilers) were randomly distributed among 4 dietary treatments with 5 replicate pens per treatment. In T1, chickens were fed on a diet with 0·3?mg/kg added Se, without added Cd. In T2, chickens were fed on a diet with 0·3?mg/kg Se and 10?mg/kg Cd. In T3, chickens were fed on a diet with 0·3?mg/kg Se and 100?mg/kg of Cd added and in T4 treatment, chickens were fed on a diet with 3?mg/kg Se and 100?mg/kg Cd added. Se was added as Se-yeast. Cd was added as cadmium chloride (CdCl2). On d 28 and 42, two chickens per replicate pen were killed for collection of whole blood, liver, kidney and breast muscle samples. Samples were analysed by ICP-MS. The data were analysed using a multivariate linear model.

3. While low Cd concentrations in the diet led only to an increase of Cd concentration in the examined tissues, addition of high concentrations of Cd increased the concentration of Cd, Cu, Sb and V and decreased that of Se, Mn and Fe. Addition of high Se concentrations did not significantly reduce Cd concentration.

4. Prior to model application, correlations of 78 elements were noted, while after model application 39 correlations were noted. Most notably, Cd was correlated with Ca, Co, Cu and Mg, while Se was correlated with Mn.

5. The present study revealed several correlations between essential, probably essential and toxic elements illustrating the importance of the balance between pro-oxidants and antioxidants.  相似文献   

14.
本试验旨在研究饲粮中减少铁、锰、锌、镁和铜5种矿物元素添加量对育肥猪后期生长性能、肉品质、血清生化指标以及骨骼肌矿物元素含量的影响。选用体况良好、体重接近[(76.17±1.58)kg]的"杜×长×大"三元杂交育肥猪300头,随机分成3个组,每组10个重复,每个重复10头猪,公母各占1/2。对照组饲喂基础饲粮,试验组饲喂在基础饲粮基础上减少30%或60%矿物元素的饲粮。预试期3 d,正试期30 d。结果表明:与对照组相比,1)饲粮减少30%和60%矿物元素对育肥猪的生长性能和肉品质无显著影响(P0.05);2)饲粮减少30%和60%矿物元素,育肥猪血清氨含量显著升高(P0.05);减少60%矿物元素显著降低血清中总胆固醇的含量(P0.05),同时有降低血清总蛋白含量的趋势(P0.10);3)饲粮减少30%矿物元素对育肥猪骨骼肌中各矿物元素含量并无显著影响(P0.05),而减少60%矿物元素使得骨骼肌铜、铁和锰元素含量显著降低(P0.05)。由此得知,育肥猪饲粮中铁、锰、锌、镁和铜元素的含量可减少NRC(2012)推荐量的30%,而不会影响育肥猪后期的生长性能和肉品质。  相似文献   

15.
Reasons for performing study: There is limited information regarding the number of races and the period for evaluation of outcome which is critical for assessment of SDF tendonitis treatments. Objective: To evaluate the re‐injury rate and racing performance of Thoroughbred racehorses that sustain SDF tendonitis in relation to matched controls in terms of number of races post treatment and maximum racing performance ratings before and after injury. Study design: Clinical records and racing histories of 401 racehorses with a first occurrence of SDF tendonitis diagnosed by ultrasonography. Controls were of the same age, sex and were horses training in the same establishment at the time of injury as the case horses and where the trainer reported that the horse had not had a previous SDF tendon injury or treatment. Results: Eighty percent of both case and control horses returned to racing after the date of injury, and the re‐injury proportion within 3 years of treatment was 53%. The difference in Racing Post Rating(max) (RPR(max)) and the Racing Post Rating in the race immediately before the treatment date was significantly smaller in case horses (mean = 9.6 lbs; range = 0–75) compared to control horses (mean = 17.0 lbs; range = 0–79). No significant decrease in RPR(max) was noted post injury. No difference between case and control horses was found for return to racing and racing 3 times, but control horses were significantly more likely to compete 5 races post treatment date than case horses. Conclusion and clinical relevance: Injury was associated with an individual's pre‐injury maximum performance level and return to racing and completion of 3 races are not useful indicator of the outcome of horses with SDF tendonitis. The assessment of the outcome of horses with an SDF injury in a population of racehorses using the number of races post injury requires a minimum of 5 races post injury to be a useful indicator. Further, a re‐injury proportion in a population of horses in training for 3 years post treatment.  相似文献   

16.
The present investigation was carried out to assess the trace mineral profile in blood and hair from cows environmentally exposed to lead and cadmium and to examine if these toxic heavy metals in blood and hair could affect blood copper, cobalt, zinc and iron concentrations and their accumulation in hair. Respective blood and tail hair samples were collected from adult cows above 3 years, reared in different industrial localities. Samples were also collected from urban areas with small industrial units (n = 55) and areas supposed to be free from pollution. The concomitant exposure of animals to both the heavy metal pollutants was not recorded in either of the industrial or urban locality. Blood lead was significantly (P < 0.01) correlated with blood copper (r = -0.339), cobalt (r = -0.224) and iron (r = -0.497). The increasing blood lead concentrations, irrespective of area of collection of samples, was associated with declining blood copper and iron, and cows with blood lead level above 0.60 mug/ml had significantly (P < 0.05) lower blood copper and iron. The higher blood lead but not cadmium significantly influenced the accumulation of lead (r = 0.323, P < 0.01) and cadmium (r = 0.204, P < 0.01) in hair possibly leading to significantly (P < 0.05) higher accumulation of both lead and cadmium in hair from cattle around lead-zinc smelters and closed lead-cum-operational zinc smelter, where blood cadmium level was comparable with that from unpolluted area. Concentration of zinc (r = 0.237, P < 0.01) and iron (r = 0.183, P < 0.01) but not copper and cobalt in tail hair was significantly influenced by their respective blood concentration. Both the hair lead and cadmium had a significant (P < 0.01) positive correlation with hair copper (r = 0.234, 0.294), zinc (r = 0.489, 0.775), and iron (r = 0.385, 0.643) concentrations. Thus, it is concluded from the present study that the higher blood lead concentrations in cattle irrespective of locality/industrial operations areas affected trace elements profile in blood and hair.  相似文献   

17.
The distribution of specific gravity values for 2,599 urine samples collected from racing Thoroughbred horses that were known to have received furosemide prior to racing was compared with that for 1,669 urine samples from racing Thoroughbred horses that reportedly had not received furosemide. Values of specific gravity for furosemide-treated horses were significantly lower (P < 0.001) than those for horses that had not received furosemide, and the proportion of horses with urine specific gravity either <1.010 or <1.012 was significantly greater (P < 0.001) among the furosemide-treated horses. These data indicate that evaluation of urine specific gravity would be a useful component of drug testing programs for regulation of furosemide use.  相似文献   

18.
Genetic correlations for body measurements and conformation and functional traits in foals and studbook horses with racing traits were estimated in the Finnhorse and Standardbred. Genetic response and accuracy were estimated using records of animal, half‐sibs and parents in selection scenarios for racing traits, for foal and racing traits, for studbook and racing traits, and using records of animal, half‐sibs and parents for foal traits and racing traits of parents. Racing time and earnings were the breeding objective. Low‐to‐moderate genetic correlations for body measurements and racing traits indicated that selection favours bigger horses at all ages. Being mainly favourable for the breeding objective, genetic correlations for conformation and functional traits with racing traits were highest for the foal traits of type, trot and overall grade and for the studbook traits of character and movements. Genetic correlations for foal and studbook conformation with racing traits were low in the Finnhorse and moderate to high in the Standardbred. In foals, the highest genetic correlations were for trot with racing time (?0.54) and with earnings (0.52) in the Finnhorse, and for overall grade with racing time (?0.54) and with earnings (0.54) in the Standardbred. In studbook horses, genetic correlations were high for character with racing time and earnings in the Finnhorse (?0.68, 0.61) and in the Standardbred (?0.63, 0.70), and for movements with racing time and earnings in the Finnhorse (?0.70, 0.69) and in the Standardbred (?0.90, 0.88). To increase accuracy of conformation and functional traits, foal traits would be more useful in the index with racing traits, as being less preselected than studbook traits. The foal traits (type, trot, overall grade) having moderate heritability and genetic correlations with racing traits would be useful in multi‐trait index before a racing career, where the greatest gain is because of a shorter generation interval. It would be feasible to implement for AI stallions.  相似文献   

19.
Inductively coupled argon plasma emission spectroscopy was used to measure Al, As, Ca, Cd, Cr, Cu, Fe, Pb, Mg, Mn, Hg, Mo, P, K, Se, Na, Tl, and Zn in canine specimens (70 serum, 270 liver, and 200 kidney). Mean concentrations of each of these elements in detectable amounts in these samples were established, and histograms of the concentration distributions of elements in the samples were developed.  相似文献   

20.
微量元素对黑麦草产量和品质的影响   总被引:5,自引:1,他引:4  
苏加义  赵红梅 《草业科学》2008,25(10):66-69
在N、P、K肥料的基础上,采用Fe、Cu、Zn、Mn、B、Mo 6种微肥对黑麦草Lolium multiflorum进行叶面喷施,研究不同用量对黑麦草产量和品质的影响。试验表明:喷施Fe 0.05%、Cu 0.07%、Zn 0.40%、Mn 0.10%、B 0.60% 和Mo 0.20%与对照组相比增产差异显著(P<0.05)。适度的Fe、Cu、Mn、B和Mo能提高黑麦草的粗蛋白、粗纤维和粗灰分含量,粗蛋白提高3.72%~6.67%,无氮浸出物含量呈降低趋势,喷施Zn肥降低了粗蛋白含量,喷施B肥提高了粗脂肪含量。黑麦草中Cu、B、Mo的含量随喷肥量的提高而增加,喷施Cu 0.1%、B 1%和Mo 0.3%时,黑麦草中的含量最高,与对照组差异显著(P<0.05)。喷施Zn 0.40%、Mn 0.1%、Fe 0.05%时牧草中相应的微量元素最高,与对照组差异显著(P<0.05)。  相似文献   

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