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1.
Maize plants, grown for 7 and 21 days on a nutrient solution with NO3 ‐ as the sole nitrogen source showed a clear diurnal pattern with respect to the in vivo NRA. Especially in roots dark/light fluctuations of the enzyme activity were high. Also in NO3 ‐ uptake, OH‐ efflux and endogenous content of water soluble carbohydrates a diurnal variation was found. The plant age did not significantly affect the daily rhythm.

Because day/night changes of the in vivo root NRA and nitrate uptake were proportional, the relative content of reduced N in the xylem sap of the plants was constant during a day/night interval. At both day 7 and day 21 about 40–50% of the N was transported via the xylem as amino N. As a result of non‐synchronous variation of the specific root and shoot NRA, root reduction capacity showed a great within‐day variation. It varied between 20 and 40% of the whole plant reduction capacity. Since the ratio N‐organic to N‐total in the xylem sap was about 0.5, cycling of organic nitrogen was very likely in these maize plants.  相似文献   

2.
In herbaceous plants grown in controlled environmental conditions nitrate (NO3?) uptake increases during the day and decreases in the night. The aim of this work was to measure NO3? uptake rates along the day-night cycle, in rose (Rosa hybrida L.) plants grown under controlled environmental conditions. Two independent experiments were conducted inside a growth chamber at 20 ºC and 25 ºC, using rose mini-plants cv. Texas, grown in a hydroponic nutrient film technique (NFT) set-up with at 3.0 mol m?3 NO3? concentration. Dry matter and nitrogen (N) accumulation were registered during growth and NO3? uptake rates were measured during a day-night cycle, using 15N as 15NO3?. In both experiments the hourly estimated N-NO3? accumulation rates are near to the measured uptake rates of 15NO3? and nitrate uptake decrease during the day and increase in the night, in contrast with the herbaceous plants. Results are discussed on the basis of N plant demand and carbohydrates availability.  相似文献   

3.
Sunflower plants (Helianthus annuus) were grown in a continuous flow nutrient system, in which nitrogen was supplied, under controlled pH conditions, in either the NO3-or NH4-form. Nutrient uptake and distribution, as well as dry matter production of the plants, was followed over the growth period. The results obtained may be summarized as follows: 1. At all stages in development, growth was somewhat greater in the plants of the NO3-treatment, but the difference between the two treatments was not large. The similarity in the behaviour of plants in the two nitrogen treatments is discussed in relation to the maintenance of a high pH in the nutrient medium. 2. The mean rates of uptake of Ca, Mg, K, and Na, expressed per unit root length, were all higher in the NO3-fed plants. For P, the mean rate of uptake was higher in the NH4-fed plants. 3. The levels of K, Ca, Mg, and Na, per unit dry weight, were higher in the NO3-fed plants, but for P the converse was true. 4. The higher uptake of Ca and Mg by NO3-fed plants was reflected in the higher concentrations of these elements in the leaves. In the case of K, accumulation occurred in the roots. 5. From the results of selected harvests, it was found that total nitrogen uptake was higher in the NO3-fed plants.  相似文献   

4.
Abstract

The diurnal rhythm of release of phytosiderophores and uptake rate of zinc (Zn) was studied in iron (Fe) deficient wheat (Triticum aestivum L. cv. Ares) plants grown in nutrient solution under controlled environmental conditions. Different forms of Zn (e.g. ZnSO4, ZnEDTA) were used to obtain different degrees of loading of the root apoplasmic pool with Zn.

In the Fe-deficient plants the release of phytosiderophores from the roots followed a distinct diurnal rhythm with a steep peak about 4 h after the onset of the light period. These plants also showed a similar pattern in the rates of Zn uptake over the 24 h day-night cycle. During the light period there was a steep transient peak (factor 3.8) in Zn uptake rate in the Fe-deficient plants supplied with ZnSO4. This transient peak was much less distinct in plants supplied with ZnEDTA (factor 1.8) and absent in plants supplied with ZnEDTA plus free chelator (+ NaEDTA) in excess. The peak in Zn uptake coincided with the maximum rate of phytosiderophore release in the Fe-deficient plants. In the Fe-sufficient plants the release of phytosiderophores was very low and no such peak in Zn uptake rates could be observed.

These results demonstrate that phytosiderophores mobilize Zn not only in the rhizosphere, but also from the root apoplast. Thus, the apoplasmic pool of micronutrient cations has to be taken into account as potential source for both uptake and diurnal variation in uptake rates of Micronutrient cations.  相似文献   

5.
Abstract

Standardization of sampling methodology is essential when using plant analysis as an indicator of the nutrition status of a crop. Time of sampling during the day is a factor which was shown to affect tissue nitrate (NO3) concentration of irrigated crops. The diurnal variation of NO3 concentration in small grain cereal plants, grown under rainfed mediterranean conditions, was tested by an in situ sap test and by analysing dried tissue. Variation in NO3 concentration was shown to exist among plants grown under field conditions. Variation was also shown to exist at different hours of sampling within the day. However, these variations did not follow any diurnal trend and the magnitude of the difference in NO3 concentration did not affect the diagnosis of the nitrogen (N) nutrition status of the crop. The results suggest that diurnal variation of NO3 concentration of cereals grown under rainfed Mediterranean conditions is not a major concern in using NO3 tissue tests to predict N availability provided that sampling is done during the daylight hours.  相似文献   

6.
Zucchini squash (Cucurbita pepo L. cv. Green Magic) plants were grown hydroponically with nitrate (NO3):ammonium (NH4) ratio of 3:1 until the onset of flowering when the plants were assigned to four NO3:NH4 ratio (1:0, 1:1, 1:3, or 3:1) treatments. Changing the original nitrogen (N) form ratio significantly affected plant growth, fruit yield, nutrient element, and water uptake. Growth of plants was better when NO3‐N (1:0) was the sole form of N than when NH4‐N was part of the N treatment. Fruit yields for plants fertilized with 1:0 or 1:3 N‐form ratio were double those of plants grown continuously with 3:1 N ratio. The largest leaf area and plant water use were obtained with 1:0 N ratio treatment Total uptake of calcium (Ca), magnesium (Mg), and potassium (K) decreased with increasing NH4‐N proportion in the nutrient solution which suggest NH4‐N was competing with these cations for uptake. The results also demonstrated that growers may increase fruit yield by using a predominantly NO3‐N source fertilizer through the vegetative growth stage and by shifting the NO3:NH4 ratio during the reproductive phase.  相似文献   

7.
The experimental system described allows concomitant hourly measurements of CO2, H2O, and NO3 uptake rates by plants grown hydroponically in a greenhouse. Plants are enclosed in an airtight chamber through which air flows at a controlled speed. Carbon dioxide exchange and transpiration rates are determined from respective differences of concentrations of CO2 and water vapor of the air at the system inlet and outlet. This set‐up is based on the “open‐system”; principle with improvements made on existing systems. For instance, propeller anemometers are used to monitor air flow rates in the chamber. From their signal it is possible to continuously adjust air speed to changing environmental conditions and plant activity. The air temperature inside the system therefore never rises above that outside. Water and NO3 uptake rates are calculated at time intervals from changes in the volume and the NO3 concentration of the nutrient solution in contact with the roots. The precise measurement of the volume of solution is achieved using a balance which has a higher precision than any liquid level sensors. Nitrate concentration is determined in the laboratory from aliquots of solution sampled at time intervals. A number of test runs are reported which validate the measurements and confirm undisturbed conditions within the system. Results of typical diurnal changes in CO2, H2O, and NO3 uptake rates by fruiting tomato plants are also presented.  相似文献   

8.
The influence of two N sources, NO3 alone and NH4+NO3 (2:1) in the external medium on fluxes of Cl, N, Na and K calculated from the total nutrient content per unit root dry weight, were investigated using cucumber (Cucumis sativus) seedlings grown in saline conditions. The N source affected the fluxes of the above ions very differently: (a) when only NO3 was added, the rate of Cl uptake was inhibited whereas the rate of K absorption was stimulated; and (b) by contrast, the presence of NH, in the N source enhanced Cl uptake and inhibited K uptake. The effect on the rates of Na and N uptake was more complex and salinity dependent. The potassium: sodium ratios (K:Na) and potassium: sodium selectivity ratio (S., ) in roots and shoots in relation with the treatments are also presented. Possible reasons of the N source effect on the processes implicated with the response of plants to salinity are also discussed.  相似文献   

9.
Abstract

The effects of night temperature on biomass accumulation and plant morphology were examined in rice (Oryza sativa L.) during vegetative growth. Plants were grown under three different night temperatures (17, 22 and 27°C) for 63 days. The day temperature was maintained at 27°C in all treatments. The final biomass of the plants was greatest in the plants grown at the highest night temperature. Total leaf area and tiller number were also the greatest in this treatment. Growth analysis indicated that the relative growth rate in the 27°C night-temperature treatment was maximal between days 21–42 and this was caused by increases in leaf area ratio, leaf weight ratio and specific leaf area. Plant total nitrogen contents did not differ among treatments. However, nitrogen allocation to the leaf blades was highest and the accumulation of sucrose and starch in the leaf blades and sheaths was the lowest in the 27°C night-temperature treatment by day 42. Despite this, dark respiration was also highest, and both the gross and net rates of CO2 uptake at the level of the whole plant at day 63 were the highest in the 27°C night-temperature treatment. Thus, high night temperature strongly stimulated the growth of leaf blades during the early stage of rice plant growth, leading to increased biomass during the vegetative stage of the rice plants. As the CO2 uptake rate per total leaf area was higher, photosynthesis at the level of the whole plant was also stimulated by a high night temperature.  相似文献   

10.
Abstract

Experiments were conducted using different NO3 –/NH4 + ratios to determine the effects of these sources of N on mineral element uptake by sorghum [Sorghum bicolor (L.) Moench] plants grown in nutrient solution. The NO3 –/NH4 + ratios in nutrient solution were 200/0, 195/5, 190/10, and 160/40 mg N L–1. Nutrient solutions were sampled daily and plants harvested every other day during the 12‐day treatment period.

Moderately severe Fe deficiencies were observed on leaves of plants grown with 200/0 NO3 –/NH4 + solutions, but not on the leaves of plants grown with the other NO3 –/NH4 + ratios. As plants aged, less Fe, Mn, and Cu were translocated from the roots to leaves and leaf/root ratios of these elements decreased dramatically in plants grown with 200/0 NO3 –/NH4 + solutions. Extensive amounts of Fe, Mn, and Cu accumulated in or on the roots of plants grown with 200/0 NO3 –/NH4 + solutions. Manganese and Cu may have interacted strongly with Fe to inhibit Fe translocation to leaves and to induce Fe deficiency. As the proportion of NH4 + in solution increased, K, Ca, Mg, Mn, and Zn concentrations decreased in the leaves, and Ca, Mg, Mn, and Cu concentrations decreased in roots. Potassium and Zn tended to increase in roots as NH4 + in solution increased.  相似文献   

11.
Snap bean (Phaseoius vulgaris L.) showed an increasing preference for NO3 with increasing total N and equal ratio of NO3 to NH4 when grown in nutrient culture. The percent increase in NO3 uptake over NH4 from solution was about the same as the increase in percent K content found in the plants at harvest. Peak periods of NO3 and NH4 uptake were observed, similar to what has been found for the other vegetable legumes.  相似文献   

12.
Photosynthetic irradiance has variable effects on cotton (Gossypium hirsutum L.) growth, development, lint yield and fiber quality. However, little is known about the effect of shade on the mineral nutrient status of cotton plants. A two‐year study was conducted to determine the effects of shade (63% light reduction) at different growth stages on mineral nutrient concentrations in plant components of field‐grown cotton. Averaged over the three growth stages of first flower, peak flower, and boll development, an 8‐day period of shade increased petiole NO3‐N, phosphorus (P), potassium (K), and sulfur (S) concentrations by 145, 17, 20, and 18%, respectively. Total nitrogen (N), P, K, S, calcium (Ca), and magnesium (Mg) concentrations in the leaf blades of the sampled petioles increased 19, 29, 22, 22, 13, and 16%, respectively, compared with those of unshaded control plants. However, bracts and floral buds of 20‐day‐old squares of shaded cotton plants showed a slight decrease (6%) in total N concentration, and increases in the other mineral nutrient concentrations. The increased mineral nutrients of shaded plants were closely associated with decreased carbohydrate accumulation. Shade during plant reproductive growth significantly affected nutrient status and TNC/N ratio of cotton. This study indicated that light intensity at the time of sampling can cause non‐representative nutrient analysis and erroneous diagnostic recommendations. Therefore, the time of day and light conditions must be considered when sampling cotton petioles and other tissues for nutrient diagnoses.  相似文献   

13.
Abstract

Nitrogen‐form effect on nutrient uptake and the subsequent concentration of nutrients in turfgrass plant tissue has not been thoroughly investigated. This study evaluated the effects of clipping regime and N‐form on the tissue concentration of macronutrients and micronutrients and macronutrient uptake in ‘Penncross’ creeping bentgrass (Agrostis palustris Huds.). Turfgrass plugs were grown under greenhouse conditions in a modified Hoagland's solution with a combination of three nutrient solutions (100% NO3 ?, 100% NH4 +, and 50:50 ratio of NH4 +:NO3 ?) and two cutting regimes (cut and uncut). Concentrations of macronutrients and micronutrients were determined for shoot, root and verdure. Nutrient uptake was determined weekly. Uncut NO3 ?‐treated plants accumulated higher concentrations of K, Ca, Mg, B and Cu in the shoot tissue; P, K, Ca, Mg, B, Cu, Mn and Zn in the root tissue; and P, Ca, Mg, B, Fe and Mn in the verdure compared to uncut NN4 +‐treated plants. Nitrate uptake was greater with uncut NO3 ?‐treated plants than was NH4 + absorption with uncut NH4 +‐treated plants. Plants grown with the uncut 50:50 treatment adsorbed more NH4 + than NO3 ?. Plants grown with the uncut NO3 ? and 50:50 treatments adsorbed higher amounts of P, K, and Ca compared to the NH4 + treatment. The cut NO3 ?‐treated plants accumulated higher concentrations of K in the shoot tissue; P, Ca, Mg, B, Cu, Fe and Mn in the root tissue; and B in the verdure than did the cut NH4 +‐treated plants. Cut NO3 ?‐treated plants adsorbed less NO3 ? than did cut NH4 +‐treated plants adsorbed NH4 +. The cut 50:50 treatment adsorbed more NH4 + than NO3 ?. Plants grown with NO3 ? and 50:50 treatments, under both cutting regimes, resulted in higher concentrations of most macro‐ and micronutrients and greater nutrient uptake compared to the NH4 +‐treated plants.  相似文献   

14.
In order to reduce nutrient wastes to the environment the supply should be in accordance to the demand for these. Two experiments were conducted to study and quantify the effect of temperature, irradiance, and plant age on the uptake of nitrate (NO3?), ammonium (NH4+), dihydrogen phosphate ion (H2PO4?), potassium (K+), calcium (Ca2+), magnesium (Mg2+), and sulfate (SO42). In the first experiment, various levels of temperature and irradiance were applied to plants in a growth chamber, while in the second experiment the uptake was studied along the crop season under greenhouse conditions. The uptake rates were calculated at 2-hour intervals through sampling the nutrient solution and analyzing it by inductively coupled plasma atomic emission spectrometry (ICP-AES). Increasing light and temperature enhance the uptake rates, while the rates decrease with plant age. Nitrogen absorption was similar during the day as during the night. No differences were found in the absorption of H2PO4?, K+, Ca2+, Mg2+, and SO42? between day and night. Nitrate absorption was found to have a positive correlation with the absorption of all the ions except for NH4+.  相似文献   

15.
Chickpea plants (Cicer arietinum L cv. ILC 195) were grown for 24 days in water culture under two regimes of nitrogen nutrition (NO3 or NH4‐N) with or without Fe. For plants fed with NO3‐N, Fe stress severely depressed fresh weight accumulation and chlorotic symptoms of Fe‐deficiency developed rapidly. Little difference in growth occurred in the NH4‐fed plants, whether or not Fe was withheld, with no visual evidence of Fe‐deficiency indicating a beneficial effect of NH4 in depressing the symptoms of Fe chlorosis. Typical pH changes were measured in the nutrient solution of the control plants in relation to nitrogen supply, increasing with NO3 and decreasing with NH4‐nutrition. With both forms of nitrogen, plants acidified the nutrient solution in response to Fe‐stress. Under NH4‐nutrition, acidification was enhanced by withholding Fe. In the NO3‐fed plants the uptake of all nutrients was reduced by the stress but proportionally NO3‐ and K+ were most affected. Total anion uptake was depressed more than that of cation uptake. For the NH4‐fed plants withholding Fe resulted in an increased uptake of all ions except NH4 + which was depressed. Regardless of the form of N‐supply, when Fe was withheld from the nutrient solution the net H+ efflux calculated from the (C‐A) uptake values was closely balanced by the OH” added to the nutrient solution to compensate for the pH changes. Evidence of accumulation of organic acids in the Fe‐stressed plants was found, especially in the NO3‐fed plants, indicating a role for these internally produced anion charges in balancing cation charge in relation to the depression of NO3 ‐ uptake associated with Fe‐stress.  相似文献   

16.
The release of phytosiderophore (PS) from roots of Fe-deficient graminaceous plants follows a distinct diurnal rhythm with maximum release rates occurring usually 3 to 4 hours after the onset of light. However, it remains to be determined whether absorption of the PS-Fe3+ complex shows a diurnal rhythmicity similar to that of PS release, Barley plants grown with or without 10 µM FeEDTA for 7 days were fed with ferreted PS (10 µM labelled with 59Fe) at 4-h intervals to study the diurnal variations in the absorption and transloca tion of 59Fe, The absorption of 59Fe, irrespective of the Fe nutritional status of the plants, was higher during the day and lower during the night but did not show any peak throughout the day-night cycle. On the other hand, the translocation of 59Fe into shoots of Fe-deficient plants was lower than that of Fe-sufficient plants, while the Fe nutritional status of the plants did not affect the absorption of 59Fe by roots, The formation of root apoplastic 59Fe was lower during the day and higher during the night, regardless of the Fe nutritional status of plants. Our results showed that the absorption of the PS-Fe3+ complex by roots did not follow the PS release pattern.  相似文献   

17.
The influence of N form on xylem exudate and the guttation fluid concentration in cucumber plants was studied under greenhouse conditions. Plants were hydroponically grown with three NO3:NH4 ratios (100:0, 80:20, and 60:40) at a constant pH of 6.0 in the nutrient solutions. Plants supplied with 60:40 NO3:NH4 ratio displayed a significant decrease of NO3‐N, total‐N, organic‐P, and Mn concentrations in the xylem sap and an increase of H2PO4‐P, SO4‐S, Cl, B, and Zn concentrations. Potassium and Ca uptake in these plants was slightly reduced, indicating that pH control was an important factor for cationic nutrition in cucumber plants fed with NH4. The major ions present in the nutrient solutions are concentrated in the xylem sap, particularly for NO3, K, Ca, and Na. The NO3:NH4 ratio had a small effect on the ionic levels of the guttation fluid. The concentrations of all nutrients in the guttation fluid were substantially reduced, except for Cl, showing that the leaf tissues of cucumber plants remove the excess of Cl ion. Finally, in this study, secondary effects of N source on ion uptake and release were minimized by controlling nutrient solution pH.  相似文献   

18.
In nitrate-fed plants cycling of nitrogen (N) and potassium (K) may serve several functions including supply of the roots with nutrients needed for growth, signalling of the growth-related shoot demand for nutrients to the roots, and removal of excess K from the shoot. In the present study, cycling and recycling of N and K were estimated in plants showing different rates of shoot and root growth. To induce these variations in growth, the plants were cultured with the same optimal nutrient supply but with the root zone temperature (RZT) at 12°C or 24°C. Additionally at both RZT, the plants were grown with their shoot base including apical shoot meristem at high or low temperature (SBT). Decreasing the RZT to 12°C drastically diminished root growth and accumulation of N and K in the roots. Cycling of N and K were less reduced by low RZT. At both RZT, N and K cycling were markedly reduced at low in comparison to high SBT although root growth was not affected by the SBT. Obviously, N and K cycling from shoot to roots were more affected by shoot growth than by the growth related demand of the roots for nutrients. At both RZT, N and K cycling exceeded accumulation in the roots. It was estimated that at least 20—33% of the N, and 24—51% of the K translocated from the roots to the shoot in the xylem is not directly derived from root uptake but from cycling. Plant culture at low shoot base temperature (SBT) drastically diminished shoot growth, and the accumulation of N and K in the shoot to less than 50% of the values measured in plants grown at high SBT. The low SBT-induced decrease of N accumulation in the shoot, at both RZT was associated with a reduction of K circulation and recirculation rates to less than 50% of those found in plants grown at high SBT. These findings are in accordance with the suggested role of K+ for charge balance facilitating the transport of NO3— in the xylem and disposal of the negatively charged products of NO3— assimilation from shoot to roots in the phloem. In plants cultured at low SBT, net uptake and translocation rates of N and K were diminished to less than 50% of those measured in plants grown at high SBT. This repression was associated with reduced rates of N and K cycling from the shoot to the roots. Obviously, low rates of N and K cycling from the shoot to the roots are not necessarily signals to increase uptake in the roots. It is suggested that for plants adequately supplied with N, high rates of N cycling and recycling might be the consequence of an apparent lack in control of phloem loading of amino acids in the leaves.  相似文献   

19.
Abstract

Seedlings of four maize hybrids were grown hydroponically to investigate the impact of different N sources (Ca(NO3)2, (NH4)2SO4 and a 1:1 mixture of both) on (i) production and partitioning of root and shoot dry matter, (ii) concentration of soluble carbohydrates in roots and shoots and their partitioning to these plant parts, (iii) concentration of starch in the shoot, and (iv) N uptake. During the main phase of the experiments (duration 14d), the plants were grown in a greenhouse at 25/22°C day/night temperatures and a photoperiod of 16h. Nitrogen was supplied at three concentrations (2.8, 28, and 280 ppm). The root‐zone pH was 6.5. Under the lowest N supply, the N sources produced similar root and shoot dry matters. At the highest N level (280 ppm), NO3‐fed plants were superior. In contrast, the mixture of NH4 and NO3 ? was optimum at 28 ppm. More or less pronounced N form by N concentration interactions were also found in the concentration and distribution of soluble carbohydrates and in all remaing traits. There were almost statistically significant cultivar by N form interactions in shoot dry matter (P = 0.07) and total dry matter (P = 0.06), indicating the existence of considerable genotypic variation in sensivity to NH4‐N.  相似文献   

20.
Abstract

Six greenhouse pot experiments were conducted in which yield and nutrient uptake responses of corn (Zea mays L.) or tall fescue (Festuca arundinacea Shreb) to N, P, K, lime, or temperature variables were measured. This paper describes yield—NO3‐N and total N concentration trends in these experiments. Crop concentrations of total N and NO3‐N during early growth increased consistently with amount of applied N. These concentrations usually decreased (by dilution and assimilation) with time of growth and yield response to other growth‐limiting nutrients and other factors. Concentrations of K also increased in corn with amount of applied K, and high K was associated with higher concentrations of NO3‐N during early growth in some experiments. This was usually true only for early crop growth periods during which rates of nutrient uptake exceeded growth rate. High K concentrations may have retarded growth and NO3‐N reduction and assimilation.  相似文献   

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