共查询到20条相似文献，搜索用时 156 毫秒
1.
线粒体DNA由来的RAPD标记能鉴别水稻雄性不育和可育细胞质 总被引：9，自引：0，他引：9
以野败型细胞质的水稻雄性不育系珍汕97A及其保持系珍汕97B的总DNA为模板,从100个引物中筛选到OPA12对珍汕97A能扩增出一条1600 bp的特异带.用OPA12扩增野败型细胞质的龙特浦A及其保持系龙特浦B、矮败型细胞质的协青早A及其保持系协青早B、恢复系明恢63、珍汕97A/明恢63的F1和F2个体的总DNA,不育系、F1和F2所有调查个体都有16 相似文献
2.
以珍汕97A/明恢63的F2群体为材料,应用SSR标记对水稻野败型恢复基因Rf3进行定位。该试验从F2分离群体中筛选出119个极端不育单株组成隐性基因定位群体。针对水稻第1染色体短臂Rf3所在染色体的可能区间,应用37个SSR标记检测亲本,从16个多态性标记中挑选出9个检测定位群体。结果表明物理位置连续排列的SSR标记RM10353、RM1195和RM3746各有8个单株与Rf3基因发生了单交换,且重组子数表现为最少,据此可将Rf3定位于这3个标记的两侧标记内。因此最终将Rf3定位在相距679.9 kb的SSR标记RM10338和RM10376之间。 相似文献
3.
水稻野败型细胞质雄性不育恢复基因Rf3的定位 总被引：3，自引：0，他引：3
以珍汕97A/明恢63的F2群体为材料,应用SSR标记对水稻野败型恢复基因Rf3进行定位。该试验从F2分离群体中筛选出119个极端不育单株组成隐性基因定位群体。针对水稻第1染色体短臂Rf3所在染色体的可能区间,应用37个SSR标记检测亲本,从16个多态性标记中挑选出9个检测定位群体。结果表明物理位置连续排列的SSR标记RM10353、RM1195和RM3746各有8个单株与Rf3基因发生了单交换,且重组子数表现为最少,据此可将Rf3定位于这3个标记的两侧标记内。因此最终将Rf3定位在相距679.9 kb的SSR标记RM10338和RM10376之间。 相似文献
4.
7个籼型水稻细胞质雄性不育系异交性能的研究 总被引：3，自引：1，他引：3
以7个籼型水稻细胞质雄性不育系D62A、D63A、D64A、D23A、G46A、金23A珍汕97A为材料,视珍汕97A为对照,在海南对它们的异交性能进行了研究.结果表明:7个细胞质雄性不育系的颖花开花率从高到低依次为D23A、D63A、D62A、D64A、珍汕97A、金23A、G46A;D62A等6个细胞质雄性不育系的柱头外露率均高于对照珍汕97A;除G46A、金23A外,其余4个的午前花率均比对照珍汕97A高;D62A、D63A、D64A等3个细胞质雄性不育系的平均张颖时间均比对照珍汕97A长,其余三个短于对照珍汕97A.柱头生活力系数大小依次是:对照珍汕97A、D62A、G46A、D63A、D23A、D64A、金23A.异交结实率从高到低的顺序是D62A、D63A、D23A、金23A、D64A、G46A、珍汕97A. 相似文献
5.
利用水稻幼苗中胚轴长度和芽鞘颜色快速鉴定汕优63种子 总被引：1，自引：0，他引：1
1前言“杂交水稻种子纯度直接关系到水稻大田生产的产量,误用纯度低的伪劣种子,往往造成大幅度减产,甚至绝收,种子纯度的高低是种子质量优劣的关键性指标。快速准确地测定种子纯度是种子检验工作的一项重要内容。笔者利用水稻中胚轴长度及芽鞘颜色的高度遗传性,通过多次反复试验比较,发现根据水稻幼苗中胚轴长度及芽鞘颜色可将汕优63杂种与其亲本珍汕97A、珍汕97B、明恢63及大小青操等加以区分。2材料和方法2．1材料汕优63杂交种子、不育系珍汕97A、保持系珍汕97B、恢复系明恢63来自江苏,分别为一级种和原种,大、小青探由人工配制… 相似文献
6.
《分子植物育种》2015,(8)
本研究以5份广西普通野生稻材料DP15、DP29、DP30、DP50和DP63为供体亲本,以栽培稻品种9311为受体亲本,通过杂交及多代回交获得携带Rf3或Rf4基因座位的染色体单片段代换系8个。进一步将分别携带Rf3和Rf4基因座位的单片段代换系两两杂交、自交,筛选出同时具有这2个基因座位的双片段聚合系2个。通过野败型细胞质不育系(WA-CMS)盟抗A对单片段代换系和双片段聚合系进行恢复力测定。研究结果显示:(1)8个单片段代换系对盟抗A的恢复能力有显著不同:来自DP15的Rf3基因与来自DP15、DP30、DP50和DP63的Rf4基因具有强恢复力,它们的F1植株花粉育性达到86.18%~91.13%;来自DP29的Rf4基因具有较强的恢复力,其F1植株花粉育性为81.21%;来自DP29的Rf3基因具有弱恢复力,其F1植株花粉育性为7.00%;而来自DP30的Rf3基因没有恢复能力,其F1植株花粉育性仅为0.33%。(2)Rf3或Rf4基因的聚合效应在不同供体间表现不同:来自DP29的较强恢复基因(Rf429)与弱恢复基因(Rf329)间聚合呈现显著的累加效应;而来自DP15的2个强恢复基因(Rf315和Rf415)聚合后没有进一步提高恢复能力。研究结果表明,利用广西普通野生稻挖掘育性恢复基因是有效的,获得了多个对野败型细胞质不育系(CMS-WA)盟抗A具有强恢复力的Rf3或Rf4位点复等位基因,可供育种研究利用。 相似文献
7.
籼型红米雄性不育系红宝石的选育及其特性 总被引：4，自引：0，他引：4
为了对红米红宝石实现三系配套,回交转育了红宝石A,并对红宝石A进行了以下分析:不同细胞质来源的野败型不育系珍汕97A、印尼水田谷型不育系Ⅱ-32A、D型不育系D62A、G型不育系G46A、红宝石A(来源于D62A)育性基因具有等位性.而Bg1639、R527、R881、250(CDR22/Bg403)具有的恢复这几个不育系的恢复基因等位.通过考种分析,发现在每产量上红宝石A的F1杂种显著大于常规稻红宝石,最大每公顷能增产2 392.5kg,证明了红宝石A具有强的配合力和杂种优势,具有生产利用价值. 相似文献
8.
一个新的水稻花粉半不育性位点的定位分析 总被引：1，自引：0，他引：1
利用一套以籼稻珍汕97B为背景的粳稻日本晴染色体片段代换系,鉴定发现1个半不育的代换系。全基因组基因型分析表明,该代换系仅含3个粳稻导入片段,而其他遗传背景与珍汕97B相同。在湖北武汉和海南分别种植其衍生的F2和F3分离群体,采用单标记分析和区间作图法分析花粉育性和小穗育性的数量性状位点(QTL),结果表明,该代换系的半不育性是第2染色体上的粳稻导入片段引起的,该片段RM262~RM475区间存在1个新的影响花粉育性的QTL,其贡献率为13.9%。研究结果将为进一步精细定位水稻育性QTL以及鉴定相关功能基因提供重要的试验基础。 相似文献
9.
根据几年来杂交稻纯度海南鉴定和本地大田种植鉴定结果,汕优63组合中的杂株93％以上是珍汕97A和珍汕97 B(现简称A、B)。这两种杂株在种子外部形态上与汕优63种子无明显差异。为探讨汕优63种子室内纯度鉴定技术,研究A、B种子与汕优63种子的典型特征和区别方法,从1989年冬到1990年春,经室内反复观察鉴定,再与海南穗期鉴定结果比较,初步认为,根据种子腹白程度进行室内纯度鉴定,其方法简便易行,准确程度较高。 相似文献
10.
乙烯与水稻细胞质雄性不育的关系 总被引：10，自引：1，他引：10
从幼穗发育的IV到VII期,水稻细胞质雄性不育系(珍汕97A)幼穗和叶片的ACC含量和乙烯释放速率均高于其保持系(珍汕97B)。外施乙烯释放剂乙烯利使保持系花粉可育度明显下降;外施ACC合成酶抑制剂AVG引起两系幼穗ACC含量和乙烯释放速率下降,并使不育系花粉育性得以部分恢复,而在外施AVG的同时再施以乙烯利则AVG的恢复作用消失。 相似文献
11.
12.
广亲和粳稻恢复系选育方法 总被引：1，自引：0，他引：1
广亲和粳稻恢复系的选育除采用ＷＡ型恢复与广亲和粳稻杂交选育和不同广亲和恢复系间杂交选育外,本研究提出以粳稻为父本杂交选育的４种办法：１、以型杂交稻Ｆ１为母本转育同质广亲和恢复系;２、以ＷＡ型下育系为母本筛选同质广亲和恢复系;３、以ＷＡ型广亲和不育系为母本,筛选同质广亲和恢复系;４、以同质广亲和恢复系为母本转育新的恢复系。用上述方法已育成０２４２８等广亲和恢复系,并可同时恢复ＷＡ型和ＢＴ型胞质不育系 相似文献
13.
14.
Z. X. Fan W. X. Lei D. F. Hong J. P. He L. L. Wan Z. H. Xu P. W. Liu G. S. Yang 《Plant Breeding》2007,126(3):297-301
Over the past decade, the polima cytoplasmic male sterility ( pol CMS) three-line and two-line systems have been developed for the production of hybrid seed in Brassica napus oilseed rape in China. The discovery of the novel pol CMS restorer line FL-204 is described here. It restores male fertility of hybrid plants in the pol CMS system, but hybrid seed production can only be carried out under autumn sowing in Wuhan in south China under moderate temperatures at flowering. The restorer cannot be used as a male for hybrid seed production in northwestern China (Gansu) under spring sowing conditions, because there it is more or less male sterile due to high temperatures at flowering. Because of this behaviour, it is referred to as a fertility temperature-sensitive restorer (FTSR) in this paper. F2 , BC1 as well as double haploid populations were constructed to determine the inheritance of fertility restoration of FL-204 in the autumn at Wuhan and under spring sowing conditions at Gansu, respectively. Deviations from Mendelian genetics were observed. It was hypothesized that the change of fertility was the result of the interaction between nuclear genes [restoring gene ( Rf ) and temperature-sensitive genes ( ts )] and the cytoplasm. The Rf gene in FL-204 was incapable of restoring male fertility of pol CMS lines under spring sowing conditions at Gansu where it is inactivated by the recessive ts gene present in FL-204. However, the ts gene(s) could be non-functional under moderate temperature conditions at flowering at Wuhan which allows full expression of male fertility in FL-204. The recessive ts gene(s) can only be expressed in plants containing the pol sterile cytoplasm. A method for the utilization of the FTSR pol CMS restorer FL-204 for the production of hybrid seed in B. napus oilseed rape is proposed. 相似文献
15.
由华南农业大学选育的水稻单片段代换系S15对于野败型(WA)和矮败型(DA)细胞质雄性不育系均具有较强的恢复性。以野败型不育系博白A和矮败型不育系协青早A为母本, 单片段代换系S15为父本杂交, 采用分子标记辅助选择和连续回交的方法构建了两个BC3F2群体。利用与第1、第10染色体上恢复基因Rf3和Rf4两侧紧密连锁的SSR标记, 从这2个BC3F2群体中筛选携带基因型Rf3Rf3/rf4rf4和rf3rf3/Rf4Rf4的单株, 观察这些单株花粉和小穗育性, 并利用202个多态性SSR标记分析这些单株的遗传背景, 结果表明: (1)在同一细胞核背景下(S15), DA型细胞质的可恢复性好于WA型细胞质, 单片段代换系S15中的恢复基因Rf4的恢复力大于恢复基因Rf3的恢复力。(2)单片段代换系S15中的恢复基因对于WA型不育系博白A和DA型不育系协青早A表现出质量-数量性状的遗传。在单片段代换系S15中, 除了主效恢复基因Rf3和Rf4外, 微效基因或者修饰基因也表现出对于博白A和协青早A的恢复性作用, 而且效应较大。(3)在构建的2个BC3F2群体中, 携带基因型Rf3Rf3/rf4rf4和rf3rf3/Rf4Rf4单株的遗传背景片段数平均为1.0, 对应于恢复基因Rf3和Rf4座位的代换片段平均长度分别为12.9 cM和18.4 cM。 相似文献
16.
The genetic relationship among three cytoplasmic male sterility (CMS) systems, consisting of WA, Dissi, and Gambiaca, was
studied. The results showed that the maintainers of one CMS system can also maintain sterility in other cytoplasmic backgrounds.
The F1 plants derived from crosses involving A and R lines of the respective cytoplasm and their cross-combination with other CMS
systems showed similar pollen and spikelet fertility values, indicating that similar biological processes govern fertility
restoration in these three CMS systems. The results from an inheritance study showed that the pollen fertility restoration
in all three CMS systems was governed by two independent and dominant genes with classical duplicate gene action. Three F2 populations, generated from the crosses between the parents of good-performing rice hybrids, that possess WA, Dissi, and
Gambiaca CMS cytoplasm, were used to map the Rf genes. For the WA-CMS system, Rf3 was located at a distance of 2.8 cM from RM490 on chromosome 1 and Rf4 was located at 1.6 cM from RM1108 on chromosome 10. For the Dissi-CMS system, Rf3 was located on chromosome 1 at 1.9 cM from RM7466 and Rf4 on chromosome 10 was located at 2.3 cM from RM6100. The effect of Rf3 on pollen fertility appeared to be stronger than the effect of Rf4. In the Gambiaca-CMS system, only one major locus was mapped on chromosome 1 at 2.1 cM from RM576. These studies have led
to the development of marker-assisted selection (MAS) for selecting putative restorer lines, new approaches to alloplasmic
line breeding, and the transfer of Rf genes into adapted cultivars through a backcrossing program in an active hybrid rice breeding program. 相似文献
17.
Shivakumar Shidenur Vikram Jeet Singh Kunnummal Kurungara Vinod Subbaiyan Gopala Krishnan Surendra Kumar Ghritlahre Haritha Bollinedi Ranjith K. Ellur Brijesh Kumar Dixit Binder Singh Mariappan Nagarajan Ashok Kumar Singh Prolay Kumar Bhowmick 《Plant Breeding》2019,138(5):553-567
Hybrid rice based on wild‐abortive cytoplasmic male sterility (WA‐CMS) is important in boosting rice production, which requires diverse parents to harness heterosis. For this, exploiting the diversity of japonica through tropical japonica (TRJ) lines is an excellent route. In this study, 310 TRJ‐based new plant type (NPT) lines were developed and evaluated for Rf3 and Rf4 genes. Gene‐based (DRRM‐Rf3‐5 and DRRM‐Rf3‐10) and functional marker (RMS‐SF21‐5) targeted Rf3 locus, while gene‐linked (RM6100) and functional marker (RMS‐PPR9‐1) targeted the Rf4 locus. The frequency of the restorer allele of Rf3 gene was lower when compared to that of Rf4. Combined phenotypic and molecular screening using gene‐based and functional markers identified 42 lines that carried Rf3 and/or Rf4 genes. All the selected lines produced fertile F1s when crossed to a WA‐CMS line, “Pusa 6A”, but with varying levels of spikelet fertility. This is the first report of a marker‐cum‐phenotype‐based restorer selection using TRJ‐derived lines. Multilocation evaluation of these lines at three locations indicated better adaptation for grain yield in some of the lines. 相似文献
18.
野生稻（Oryza rufipogon）新质源雄性不育恢复系的研究 总被引：10，自引：2，他引：8
发掘野生稻（O. rufipogon）新型雄性不育细胞质源,育成新质源优质米不育系的基础上进一步研究新质源雄性不育恢复系的育种技术—FA型细胞质雄性不育恢复系定向育种。用野生稻（非轮回亲本）与籼稻品种明恢63（轮回亲本）杂交和多次回交,后代再经过自交,将野生稻中的可育基因分离、转移、重组、整合到明恢63遗传背景中,获得农艺性状似明恢63,花粉和小穗全可育不分离的野生稻新质源恢复系金恢1号。用新质源不育系与金恢1号组配两个组合,其花粉和小穗育性都恢复到正常可育水平,产量高,米质优,实现了新质源不育系三系配套应用和大幅度提高杂交稻稻米外观品质的目的。这项育种新技术可以将水稻可育基因（恢复基因）转移到任一水稻品种中育成细胞质雄性不育恢复系,突破了新质源恢复系育种的技术瓶颈,极大地提高了恢复系利用稻种资源的育种潜力,为FA型新质源优质米不育系的杂交稻育种开辟了一条崭新的途径。新型（FA）细胞质源杂交稻可能对丰富杂交稻细胞质遗传多样性、提高杂交稻亲本对稻种资源的利用潜力、以及实质性提高杂交稻的稻米品质和产量水平都将产生积极和深远的影响。 相似文献
19.
滇型水稻细胞质雄性不育恢复基因Rf-D1（t）的克隆与遗传特性分析 总被引：1，自引：1，他引：0
CMS/Rf测交F1结实率与花粉可育率检测表明,Ansanbyeo和南34对三种不同细胞质来源的粳稻不育系(CMS-DT1,CMS-BT和CMS-HL)都有较强的恢复力。根据已公布的水稻基因组序列,在水稻恢复基因定位区段内设计引物,确定出一对与两个滇型CMS的恢复系Ansanbyeo和南34恢复基因紧密连锁的分子标记引物M43804和M43558,首次克隆了滇型恢复基因Rf-D1(t),编码16个PPR蛋白。序列比对表明,该基因与BT型恢复基因Rf-1相似度达99%,两个恢复基因之间在ORF区仅存在一个碱基差异,即Rf-D1(t)第1149bp位的A变成Rf-1的C,并产生一个氨基酸的改编即K(赖氨酸)变成N(天冬酰胺)。该差异处于一个PPR结构域中,暗示该氨基酸是恢复基因的关键位点之一,它的改变可能影响对水稻CMS育性恢复力的变化。 相似文献
20.
Development and use of a two-gene marker-aided selection system for fertility restorer genes in rice 总被引：2，自引：0，他引：2
Majid Sattari Arumugam Kathiresan Glenn B. Gregorio Jose E. Hernandez Tamerlane M. Nas Sant S. Virmani 《Euphytica》2007,153(1-2):35-42
We have established marker-aided selection strategies for the two major Rf genes (Rf3 and Rf4) governing fertility restoration of␣cytoplasmic-genetic male sterility (CMS) in rice. Polymorphisms between restorer and
non-restorer␣lines were observed using RG140/PvuII for Rf3 located on chromosome 1 and S10019/BstUI for Rf4 located on chromosome 10. DNA polymorphisms associated with these two loci in restorer lines of wild abortive (WA), Dissi, and Gambiaca cytoplasm are
conserved, suggesting that similar biological processes control pollen fertility in this diverse cytoplasm. Because of their
close linkage to Rf genes and distinct banding patterns, STS markers RG140/PvuII and S10019/BstUI are well suited for marker-aided selection, enhanced backcross procedures, and pyramiding of Rf genes in agronomically superior non-restorer lines. The combined use of markers associated with these two loci improved the
efficiency of screening for putative restorer lines from a set of elite lines. Positional analyses of Rf4 and the inheritance pattern of the polymorphism in S10019/BstUI suggest that Rf4, governing fertility restoration in WA-CMS in rice, is likely to be the same gene governing fertility restoration in BT-
and HL-CMS that has a gametophytic effect, which explains why 100% pollen fertility in hybrids is impossible to attain. 相似文献