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1.
四川省松潘县牦牛体表蜱、高原鼠兔携带巴尔通体和无形体的PCR检测与进化分析 总被引：1，自引：1，他引：0
旨在了解四川省松潘县牦牛体表蜱、高原鼠兔巴尔通体和无形体感染情况。采集牦牛体表的蜱和捕获高原鼠兔，对蜱进行形态学初步鉴定后，提取蜱和高原鼠兔脾总DNA，PCR扩增蜱16S rRNA、巴尔通体rpoB和无形体16S rRNA基因，对PCR产物阳性产物测序、比对及构建系统进化树，从而确定蜱种类及蜱和高原鼠兔感染巴尔通体和无形体的种类及感染率。结果显示：在松潘县进安乡、山巴乡、下八寨乡各采集到蜱102、97和131只，共计330只，经鉴定均为青海血蜱。蜱巴尔通体仅检出1种巴尔通体，与B.melophagi亲缘关系最近，进安乡、山巴乡和下八寨乡检出率分别为16.7%、8.2%和18.3%，其中下八寨乡检出率显著高于进安乡（P<0.05）；蜱源无形体进安乡、山巴乡和下八寨乡检出率分别为9.8%、12.4%和26.7%，下八寨乡检出率显著高于进安乡（P<0.01），检出的无形体均为1种，与牛无形体（A.bovis）亲缘关系最近；下八寨乡检出的鼠兔源巴尔通体与B.queenslandens亲缘关系最近，感染率为6.7%；进安乡、山巴乡和下八寨乡检出的鼠兔源巴尔通体与B.grahamii亲缘关系最近，感染率分别为8.7%、17.9%和13.3%，3个点检出率无显著差异；未定种Bartonella sp.（MN296294）和Bartonella sp.（MN296293）仅分别在进安乡和下巴乡检出；与蜱均检出无形体不同，高原鼠兔均未检出无形体。此外，蜱和高原鼠兔均未发现2种及2种以上病原混合感染。松潘县青海血蜱携带巴尔通体和无形体，高原鼠兔感染巴尔通体，且首次在高原鼠兔体内检测到疑似B.queenslandens的病原体，提示当地居民有感染这两类病原风险。 相似文献
2.
西藏羊蜱蝇中巴尔通体检测及gltA基因序列分析 总被引：1，自引：1，他引：0
为了明确西藏部分地区绵羊体外寄生羊蜱蝇携带病原巴尔通体的感染情况,作者于2019年1-9月采集了西藏林芝、日喀则、那曲地区绵羊体外寄生羊蜱蝇298只,通过形态学鉴定、PCR扩增羊蜱蝇18S rRNA基因进行虫体鉴定,并对病原巴尔通体的gltA基因进行检测,将部分阳性PCR产物连接pMDTM-18T后转入DH5α感受态细胞,将阳性结果进行测序并进行遗传进化分析。结果显示,雌性阳性率49.8%（113/227）,雄性阳性率42.3%（30/71）,雌雄之间差异不显著（χ2=0.944,P=0.267）;羊蜱蝇携带病原巴尔通体的总感染率为48.0%（143/298）,林芝极显著高于日喀则、那曲地区（χ2=13.801,P<0.01;χ2=17.067,P<0.01）,日喀则和那曲地区无显著差异（χ2=0.084,P=0.771）;散养模式羊蜱蝇携带阳性率44.3%（102/230）,圈养阳性率85.0%（34/40）,屠宰场阳性率23.3%（7/30）,宿主散养和圈养、屠宰场存在极显著差异（χ2=20.929,P<0.01;χ2=24.38,P<0.01）,宿主散养和屠宰场存在显著差异（χ2=3.989,P=0.046）;将测序结果上传GenBank数据库获得3个巴尔通体gltA基因登录号（MN623006、MN623007、MN623008）;序列比对表明和云南、新疆巴尔通体相似性为99.6%~100%。本次研究首次检测出西藏羊蜱蝇携带病原巴尔通体,为了解西藏绵羊体外寄生虫携带病原巴尔通体和病原防控提供依据。 相似文献
3.
《畜牧兽医学报》2020,(5)
为了明确西藏部分地区绵羊体外寄生羊蜱蝇携带病原巴尔通体的感染情况,作者于2019年1—9月采集了西藏林芝、日喀则、那曲地区绵羊体外寄生羊蜱蝇298只,通过形态学鉴定、PCR扩增羊蜱蝇18S rRNA基因进行虫体鉴定,并对病原巴尔通体的gltA基因进行检测,将部分阳性PCR产物连接pMD~(TM)-18T后转入DH5α感受态细胞,将阳性结果进行测序并进行遗传进化分析。结果显示,雌性阳性率49.8%(113/227),雄性阳性率42.3%(30/71),雌雄之间差异不显著(χ~2=0.944,P=0.267);羊蜱蝇携带病原巴尔通体的总感染率为48.0%(143/298),林芝极显著高于日喀则、那曲地区(χ~2=13.801,P0.01;χ~2=17.067,P0.01),日喀则和那曲地区无显著差异(χ~2=0.084,P=0.771);散养模式羊蜱蝇携带阳性率44.3%(102/230),圈养阳性率85.0%(34/40),屠宰场阳性率23.3%(7/30),宿主散养和圈养、屠宰场存在极显著差异(χ~2=20.929,P0.01;χ~2=24.38,P0.01),宿主散养和屠宰场存在显著差异(χ~2=3.989,P=0.046);将测序结果上传GenBank数据库获得3个巴尔通体gltA基因登录号(MN623006、MN623007、MN623008);序列比对表明和云南、新疆巴尔通体相似性为99.6%～100%。本次研究首次检测出西藏羊蜱蝇携带病原巴尔通体,为了解西藏绵羊体外寄生虫携带病原巴尔通体和病原防控提供依据。 相似文献
4.
试验旨在了解新疆昆玉市某牧场绵羊体外寄生虫羊蜱蝇所携带立克次体的感染情况。从该牧场绵羊体表采集羊蜱蝇82只,经形态学鉴定后,提取羊蜱蝇DNA,对立克次体17 kDa基因和geneD基因运用分子生物学技术进行PCR扩增,测序结果采用序列分析软件MEGA7.0构建系统进化树并进行遗传进化分析。结果显示,采集物种为羊蜱蝇并检测到其携带立克次体,经鉴定为暂定巴布瑞立克次体（Candidatus Rickettsia barbariae）。研究表明,新疆昆玉市某牧场绵羊羊蜱蝇携带立克次体,结果可为新疆地区羊蜱蝇携带的立克次体病原提供一定的数据支撑。 相似文献
5.
为了对陕西省和辽宁省部分地区羊源蜱类及其携带病原体的流行情况进行分析,本试验采集了288份羊源蜱样本,采用形态学观察鉴定蜱种;采用分子生物学方法扩增巴贝斯虫18S rRNA、伯氏疏螺旋体16S rRNA、斑点热群立克次体ompA和无形体16S rRNA的基因序列,并结合核苷酸序列分析,确定所收集蜱样本中各类病原体的流行情况。结果显示,经鉴定288份羊源蜱样本均为长角血蜱。陕西省144份长角血蜱中携带巴贝斯虫、斑点热群立克次体和无形体,感染率分别为0.69%、21.53%和77.78%,且巴贝斯虫和斑点热群立克次体均与无形体复合感染;其中长角血蜱中携带Babesia microti、Candidatus Rickettsia longicornis、Uncultured Rickettsia sp. QH-122、Rickettsia endosymbiont of Haemaphysalis longicornis、Uncultured Anaplasma sp. ZJ06/2009、Anaplasma marginale、Anaplasma capra共7种病原体。辽宁省144份长角... 相似文献
6.
为了解四川省九龙县牦牛体表寄生蜱的种类及其斑点热群立克次体（SFGR）的感染情况。采集牦牛体表的蜱,经形态学初步鉴定后,提取蜱总DNA,PCR扩增蜱ITS-2基因及SFGR omp A、omp B基因,并对扩得的阳性产物进行测序和构建进化树分析,从而确定蜱及其携带SFGR的种类。在九龙县3个乡镇共采集到蜱585只,其中微小扇头蜱占52.65%(308/585)、卵形硬蜱占32.99%(193/585)、锐跗硬蜱和西藏革蜱分别占8.89%(52/585)和5.50%(32/585)。所有蜱中有374只检出SFGR,总感染率为63.93%(374/585),其中半农牧区（70.60%）的感染率极显著的高于纯牧区（45.10%）(P<0.01)。本研究首次对九龙县牦牛体表寄生的蜱虫种类及SFGR流行情况进行了调查,蜱传SFGR感染率较高且感染的SFGR主要为饶氏立克次体。 相似文献
7.
为了阐明青海海北地区患病藏羊携带蜱虫种类及蜱传病原的情况,采用PCR方法对蜱虫种类进行鉴定,并利用16S rRNA基因进行遗传进化分析,同时也利用PCR方法检测了携带的病原,对鉴定的病原进行分析。结果显示:青海海北地区主要流行西藏革蜱、森林革蜱、青海血蜱和草原革蜱,鉴定的蜱虫基因与GenBank中的参考基因的同源性均在97.00%～100.00%之间;遗传进化分析显示鉴定的蜱虫均与相应的蜱种聚类,且均与西北地区的分离株聚在同一分支上,呈现区域聚集性;蜱传病原种类丰富,包括尤氏泰勒虫、吕氏泰勒虫、绵羊泰勒虫、巴尔通体、绵羊无浆体、立克次体、贝纳特氏立克次体,流行的主要病原是绵羊无浆体和巴尔通体。通过本次的分子生物学鉴定研究,有助于研究我省蜱的生物学多样性和进化关系,丰富蜱种分子标记相关基因序列信息;同时对蜱传病原进行了分子鉴定,有助于查清蜱携带的病原谱,可为我省蜱传病的防控提供科学依据。 相似文献
8.
笔者发现当年产的羔羊羊蜱蝇感染率较高,为此于1988年4月初应用30ppm的溴氰菊酯对唐乃亥乡11群杂种绵羊(成年羊1890只、羔羊945只;感染率100%)进行了喷雾试验,结果对羊蜱蝇杀虫效果为100%,羊只安全,并认为产羔后20天左右是防治绵羊外寄生虫的最佳时机。 相似文献
9.
《中国兽医杂志》2016,(8)
了解中哈边境新疆艾比湖湿地游离蜱中斑点热群立克次体的感染情况。利用斑点热群立克次体外膜蛋白A(ompA)基因对艾比湖湿地游离亚洲璃眼蜱、边缘革蜱、血红扇头蜱进行PCR检测,并对阳性样本进行测序和BLAST序列分析,并应用Mega5.0软件建立分子系统进化树。结果中哈边境地区艾比湖湿地游离蜱斑点热群立克次体电泳阳性率36.84%(56/152),BLAST分析显示,艾比湖湿地游离蜱斑点热群立克次体基因型为Rickettsia raoultii,与从匈牙利患蜱传染的病人肿大淋巴结中分离获得的R.raoultii关系最近,处于同一分支(Gen Bank登录号为JQ798904)。结论是新疆艾比湖湿地游离蜱斑点热立克次体感染较为严重,存在立克次体自然疫源地,应尽快制定防制措施,以免危机动物及人类健康。 相似文献
10.
《中国兽医杂志》2019,(12)
为了明确青海省天峻地区青海血蜱传斑点热群立克次体带菌率及遗传进化特点,根据已发表的斑点热群立克次体外膜蛋白OmpA基因序列设计特异性引物,采用PCR方法对青海省天峻地区青海血蜱进行斑点热群立克次体检测,并对测定序列进行遗传进化分析。结果显示,在316个青海血蜱标本样品池中共检测到31个阳性样品,斑点热群立克次体(SFGR)带菌率为9.8%。遗传进化分析显示,TJ013与立克次体福建分离株FUJ98(AF169629)、黑龙江立克次体HL-93(AF179364)、HL-054(AF179362)及日本立克次体YM(U43795)处于同一分支;SFGR Omp A基因序列同源性分析结果显示,TJ013与立克次体福建分离株FUJ98(AF169629)同源性最高,为95.49%;与黑龙江立克次体绥芬分离株HLJ-054和虎林分离株HL-93的同源性均为91.44%;和日本立克次体YM同源性为90.24%。表明青海省天峻地区青海血蜱传斑点热群立克次体感染较为严重,应尽快制定防制措施,以免危及动物及人类健康。 相似文献
11.
Breitschwerdt EB 《Veterinary immunology and immunopathology》2008,123(1-2):167-171
Bartonella species are important emerging zoonotic pathogens. Transmission of these organisms in nature may be much more complex than is currently appreciated. Cats can be infected with five Bartonella species, including, Bartonella henselae, Bartonella clarridgeae, Bartonella bovis, Bartonella koehlerae and Bartonella quintana. In addition to cats, numerous domestic and wild animals, including bovine, canine, human, and rodent species can serve as chronically infected reservoir hosts for various intra-erythrocytic Bartonella species. In addition, an increasing number of arthropod vectors, including biting flies, fleas, keds, lice, sandflys and potentially ticks have been implicated in the transmission of various Bartonella species to animals or human beings. In the reservoir host, Bartonella species cause chronic intra-erythrocytic and vascular endothelial infections, with a relapsing bacteremia documented in experimentally infected cats. Although the immunopathology induced by Bartonella infection requires additional study, the organisms can localize to the heart valve (endocarditis), cause granulomatous inflammation in lymph nodes, liver or spleen, induce central nervous system dysfunction with or without cerebrospinal fluid changes, and may contribute to inflammatory polyarthritis. Hematological abnormalities are infrequent, but thrombocytopenia, lymphocytosis, neutropenia, and eosinophilia have been reported in B. henselae-infected cats. Serology, PCR and culture can be used to support a diagnosis of feline bartonellosis, however, due to the high rate of sub-clinical infections among various cat populations, documenting causation in an individual cat is difficult, if not impossible. Response to treatment can be used in conjunction with serology or organism isolation to support a clinical diagnosis of feline bartonellosis. As fleas are involved in the transmission among cats, the use of acaracide products to eliminate fleas from the environment is of critical importance to decrease the risk of B. henselae transmission among cats and to humans. 相似文献
12.
Kidd L Maggi R Diniz PP Hegarty B Tucker M Breitschwerdt E 《Veterinary microbiology》2008,129(3-4):294-303
Spotted Fever Group Rickettsia is important cause of emerging and re-emerging infectious disease in people and dogs. Importantly, dogs can serve as sentinels for disease in people. Sensitive and specific diagnostic tests that differentiate among species of infecting Rickettsia are needed. The objective of this study was to develop a sensitive and specific PCR that differentiates SFG Rickettsia infecting dog blood. Conventional and real-time PCR assays were developed using primers that targeted a small region of the ompA gene. Their sensitivity, determined by testing a cloned target sequence in the presence of host DNA, was 15–30 and 5 copies of DNA, respectively. Testing of Rickettsia cultures and analysis of Rickettsia gene sequences deposited in GenBank verified DNA could be amplified and used to differentiate species. DNA from the blood of infected dogs was also tested. Importantly, Rickettsia DNA was detected before seroconversion in some dogs. The species of infecting Rickettsia was also identified. We conclude these assays may assist in the timely diagnosis of infection with SFG Rickettsia. They may also facilitate the discovery of novel SFG Rickettsia infecting dogs, and in the investigation of dogs as sentinels for emerging rickettsioses. 相似文献
13.
Kosoy MY Saito EK Green D Marston EL Jones DC Childs JE 《Comparative immunology, microbiology and infectious diseases》2000,23(4):165-238
A large number of Bartonella species and genetic variants were compared for their ability to cause bacteremia in different rodent species: the cotton rat (Sigmodon hispidus), white-footed mouse (Peromyscus leucopus), BALB/c mouse and Wistar rat. Experimental data supported field observations that host specificity can occur among certain Bartonella species and rodent species. Bacteremia could only be readily produced in cotton rats or white-footed mice if the strains used for inoculation were originally obtained from the same species or from a phylogenetically close species. A few Bartonella colonies could be observed in the blood of some BALB/c mice by 7 days after inoculation, but no evidence of the persistence of the infection was found. Host specificity suggests the possibility of a long co-speciation of Bartonella species with their rodent hosts. Host–parasite relationships measured by the duration and level of bacteremia and the minimal infectious dose may serve as additional criteria for classification of Bartonella isolates obtained from natural environments. 相似文献
14.
Hoar BR Chomel BB Rolfe DL Chang CC Fritz CL Sacks BN Carpenter TE 《Preventive veterinary medicine》2003,56(4):299-311
Zoonotic transmission of sylvatic plague caused by Yersinia pestis occurs in California, USA. Human infections with various Bartonella species have been reported recently. Coyotes (Canis latrans) are ubiquitous throughout California and can become infected with both bacterial agents, making the species useful for surveillance purposes. This study examined the geographic distribution of 863 coyotes tested for Y. pestis and Bartonella vinsonii subsp. berkhoffii serologic status to gain insight into the natural history of B. vinsonii subsp. berkhoffii and to characterize the spatial distribution of the two agents.
We found 11.7% of specimens positive to Y. pestis and 35.5% positive to B. vinsonii subsp. berkhoffii. The two pathogens had distinct spatial clusters: Y. pestis was more prevalent in eastern portions of the state and B. vinsonii subsp. berkhoffii in coastal regions. Prevalence of Y. pestis increased with increasing elevation, whereas prevalence of B. vinsonii subsp. berkhoffii decreased with increasing elevation. There were differences in the proportions of positive animals on a yearly basis to both pathogens. 相似文献
15.
肉羊养殖作为云南省藏区畜牧业的重要组成部分,是农牧民增收致富的重要来源。为了解维西县肉羊产业的现状,采用入户走访与问卷调查的方式,对所辖的10个乡（镇）47个村委会128个农户进行调查发现,维西县肉羊养殖还存在缺乏有效的肉羊遗传改良规划、养殖方式落后、规模化程度低、养殖效率及效益不高等问题,提出今后要结合实际加大地方特色品种的收集和保护、加大良种肉羊的引进及推广力度、加大标准化适度规模养殖、加大技术研发与推广体系的建设等一系列产业发展的建议。 相似文献
16.
为了深入探究HSP27基因在藏羊卵泡发育过程中的作用,本研究选择健康的（3~4岁）经产藏母羊6只,屠宰获得卵巢组织进行藏羊HSP27基因克隆,利用生物信息学方法分析HSP27基因CDS区序列,构建HSP27基因过表达及沉默载体,分离培养藏羊卵巢颗粒细胞,将构建的载体按不同分组进行细胞转染试验即空白组、过表达载体组、沉默载体组、阴性对照组,每组设3个复孔。显微镜观察各转染组培养0、24、48、72 h的细胞形态变化及细胞计数,RT-PCR检测各转染组HSP27、GDF9、BMPR-1B、Erβ基因mRNA表达量。结果,试验成功克隆藏羊HSP27基因,其CDS序列长度为618 bp,编码203个氨基酸。试验成功构建藏羊HSP27基因过表达及沉默载体;将构建的载体转染至卵巢颗粒细胞,随着培养时间的递增各转染组间细胞形态发生改变,过表达载体组细胞由长梭状逐渐变为不规则多边形,细胞核变形分解,沉默载体组细胞周边伪足伸出减少,细胞皱缩大量凋亡;细胞计数结果显示,转染后培养72 h时,沉默载体组细胞数量极显著低于其他3组（P<0.01）,过表达载体组细胞数量显著低于空白组和阴性对照组（P<0.05）。RT-PCR结果显示,过表达载体组细胞HSP27基因mRNA表达量极显著高于空白组（P<0.01）,沉默载体组细胞HSP27基因mRNA表达量极显著低于阴性对照组（P<0.01）,过表达载体组细胞的GDF9、BMPR-1B、Erβ基因mRNA表达量均极显著高于空白组（P<0.01）,沉默载体组细胞Erβ基因mRNA表达量极显著低于阴性对照组（P<0.01）。由此可初步推测,当HSP27基因过表达时能够促进颗粒细胞增殖分化,当沉默HSP27基因时可能会触发颗粒细胞凋亡进而影响卵泡发育,以上研究成果可为HSP27基因在藏羊卵泡发育过程中的功能研究奠定试验基础。 相似文献
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试验旨在调查甘孜藏族自治州（甘孜州）全部18个县（市）牦牛梨形虫的感染情况。2018年6月至11月采集甘孜州18个县（市）临床健康牦牛全血1 381份,采用靶向18S rRNA的巢式PCR进行梨形虫核酸检测,并挑选梨形虫核酸检测阳性样本PCR产物共计124份测序鉴定虫种。结果显示,1 381份牦牛样本中梨形虫核酸阳性样本438份,平均阳性率为31.72%,其中半农半牧业县1 093份牦牛样本中梨形虫核酸阳性样本385份,平均阳性率为35.22%,纯牧业县288份牦牛样本中梨形虫核酸阳性样本53份,平均阳性率为18.40%;124份牦牛梨形虫核酸阳性样本中鉴定出感染的虫种有中华泰勒虫76份、吕氏泰勒虫25份、东方泰勒虫3份、绵羊泰勒虫2份、双芽巴贝斯虫1份和水牛泰勒虫1份,其中中华泰勒虫感染率为61.29%,吕氏泰勒虫感染率为20.16%。本试验结果表明,甘孜州牦牛梨形虫感染率较高,不同地区之间存在差异,优势虫种为中华泰勒虫和吕氏泰勒虫,为甘孜州牦牛梨形虫病防控提供了重要参考。 相似文献
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After encountering antigen, dendritic cells (DC) must differentiate into a fully mature phenotype to induce a protective, lasting T cell immunity. Paratuberculosis is a disease caused by the intracellular pathogen Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) and is characterized by a transient cell mediated immune response, that when dissipates correlates to the onset of clinical disease. In order to study the mechanism of early cellular immunity associated with M. paratuberculosis infection, we tested the hypothesis that M. paratuberculosis infected bovine DC have impaired activation and maturation thus are defective in the initiation of a sustainable and protective Th1 immune response locally. Our results demonstrate that M. paratuberculosis infected DC showed decreased endocytosis of ovalbumin, indicating some functional maturation. Co-stimulatory molecules CD40 and CD80 mRNA expression from M. paratuberculosis infected DC was increased over untreated immature DC. M. paratuberculosis infection induced chemokine receptor CCR7 increase in DC, yet CCR5 remained high. MHC II surface expression remained low on M. paratuberculosis infected DC. M. paratuberculosis infection inhibited pro-inflammatory cytokine IL-12 production and promoted IL-10 secretion by bovine DC. Together, our findings showed evidence of phenotypic and functional maturation of DC. However, we did not see the expected antigen presentation via MHC II and cytokine responses as a fully mature DC. This may suggest semi-mature DC phenotype induced by M. paratuberculosis infection. 相似文献