共查询到18条相似文献，搜索用时 203 毫秒
1.
本试验旨在研究骨桥蛋白（osteopontin,OPN）在长白公猪生殖细胞中的表达和定位,并探究其与公猪繁殖性能的相关性。试验采集了长白公猪精液和不同阶段（3日龄、3月龄、6月龄和12月龄）的睾丸组织,通过蛋白印迹的方法检测OPN蛋白在精液和不同月龄睾丸中的表达,通过免疫组化的方法对OPN蛋白在公猪睾丸细胞中进行定位;同时,根据配种胎次≥ 20胎,3次配种公猪为同一头的标准,筛选并采集17头长白种公猪精液,统计相对应的1 388头母猪的生产成绩,计算得到公猪繁殖性能指标（包括窝产总仔数、窝产活仔数、分娩率和繁殖力）。低温离心精液分离得到精子和精浆,丙酮法提取精浆蛋白,Lysis buffer方法提取精子蛋白,最后运用BCA和ELISA的方法检测精子和精浆中OPN蛋白的含量,分析OPN蛋白与公猪繁殖性能的相关性。蛋白印迹结果显示,OPN在精子、精浆和各月龄阶段的长白公猪睾丸中均以两种形式表达（67.4和33.7 ku）,且67.4 ku的形式在3月龄公猪睾丸中表达量最高;免疫组化的结果显示,OPN在长白公猪的初级精母细胞、次级精母细胞和精子细胞中表达,在精母细胞、支持细胞和间质细胞中无表达;BCA和ELISA结果显示,精子中的OPN蛋白含量是精浆中的7倍（P<0.05）,精液中的OPN蛋白与公猪窝产活仔数显著正相关（P<0.05）。本试验结果表明,OPN在各阶段的长白公猪睾丸中都有表达,且在精子和精浆中也有表达,这可能与公猪的繁殖性能有关,从而为后期OPN蛋白在公猪受精力的研究奠定基础。 相似文献
2.
《中国畜牧兽医》2020,(8)
本试验旨在研究骨桥蛋白(osteopontin,OPN)在长白公猪生殖细胞中的表达和定位,并探究其与公猪繁殖性能的相关性。试验采集了长白公猪精液和不同阶段(3日龄、3月龄、6月龄和12月龄)的睾丸组织,通过蛋白印迹的方法检测OPN蛋白在精液和不同月龄睾丸中的表达,通过免疫组化的方法对OPN蛋白在公猪睾丸细胞中进行定位;同时,根据配种胎次≥20胎,3次配种公猪为同一头的标准,筛选并采集17头长白种公猪精液,统计相对应的1 388头母猪的生产成绩,计算得到公猪繁殖性能指标(包括窝产总仔数、窝产活仔数、分娩率和繁殖力)。低温离心精液分离得到精子和精浆,丙酮法提取精浆蛋白,Lysis buffer方法提取精子蛋白,最后运用BCA和ELISA的方法检测精子和精浆中OPN蛋白的含量,分析OPN蛋白与公猪繁殖性能的相关性。蛋白印迹结果显示,OPN在精子、精浆和各月龄阶段的长白公猪睾丸中均以两种形式表达(67.4和33.7 ku),且67.4 ku的形式在3月龄公猪睾丸中表达量最高;免疫组化的结果显示,OPN在长白公猪的初级精母细胞、次级精母细胞和精子细胞中表达,在精母细胞、支持细胞和间质细胞中无表达;BCA和ELISA结果显示,精子中的OPN蛋白含量是精浆中的7倍(P0.05),精液中的OPN蛋白与公猪窝产活仔数显著正相关(P0.05)。本试验结果表明,OPN在各阶段的长白公猪睾丸中都有表达,且在精子和精浆中也有表达,这可能与公猪的繁殖性能有关,从而为后期OPN蛋白在公猪受精力的研究奠定基础。 相似文献
3.
本研究旨在分析影响大白猪繁殖性能因素(批次管理体系和精液类型)与遗传参数估计,为目标群体的遗传评估及生产管理奠定理论基础。利用DMU和ASREML软件分析2 026头大白母猪共4 512窝繁殖记录,涉及4个繁殖性状(总产仔数、产活仔数、21日龄窝重和断奶仔猪数)。结果表明:大白猪4个繁殖性状的遗传力估计值均在0.10左右,各性状间均成强正遗传相关(0.54以上)。精液类型(鲜精和冻精)效应对断奶仔猪数和21日龄窝重影响不显著(P>0.05),对总产仔数和产活仔数影响显著(P<0.05),其中冻精在总产仔数和产活仔数上较鲜精均少1头左右(P<0.05)。批次管理体系研究表明,由单周批次管理体系转变为4周批次管理体系后,母猪繁殖性能有所下降,产活仔数、断奶仔数猪和21日龄窝重分别下降0.51头、0.61头和10.13 kg。 相似文献
4.
随着现代养猪业的发展,养猪规模越来越大,其繁殖效率也就越容易受到影响而下降,进而影响生产效益,然而提高生产效益的两个关健因素就是精液品质和母猪繁殖力。为了探讨精液品质对母猪繁殖力的影响,本试验统计了河南省驻马店诸美种猪场2006~2013年母猪配种、产仔记录,分析了猪精液质量(精子活率和精子密度)与母猪繁殖力(窝产仔数和初生窝重)的相关性。结果表明:(1)产仔性能最好的是长白猪,大白猪次之,杜洛克猪最差,三个品种的平均窝产仔数分别是10.52头、9.96头、9.05头;第3胎达到最大值,其产仔数分别为11.25头、10.23头、9.57头。(2)精子活率与杜洛克、长白、大白种猪的窝产总仔数、初生窝重呈极显著相关(P0.01)。(3)精子密度与杜洛克、大白种猪窝产总仔数和初生窝重呈极显著相关(P0.01),与长白种猪的没有显著相关(P0.05),表明影响母猪繁殖成绩的关键因素是精子活率。 相似文献
5.
为探讨不同配种方式对二元母猪产仔数的影响,以杜洛克猪、长白猪、大白猪、皮特兰猪四个品种公猪精液采用人工授精的方法对11654头长大二元母猪配种,对所产11654窝总产仔数、窝产活仔数、窝损失等进行统计和分析,分析不同配种模式对二元母猪繁殖性能的影响。结果表明,配种次数、不同品种公猪组合配种对二元母猪总产仔数、产活仔数以及窝损失均无显著影响（P〉0.05）,但杜洛克公猪所配二元母猪的总产仔数和窝产仔数均高于大白和长白猪（P〈0.05）;采用长×杜×大组合配种的二元母猪总产仔数和窝产活仔数均高于其它三种组合。试验结果表明,在生产中,可在长白、大白、杜洛克猪中任选两个品种,通过二次配种的方式提高母猪的产仔数。 相似文献
6.
【目的】探究品种、公猪出生胎次、公猪同窝仔猪数、公猪乳头数、采精季节、采精月龄和采精间隔对猪精液品质的影响,以及不同品种对精液质量稳定性的影响。【方法】选取909头杜洛克、长白、大白种公猪为试验群体,收集2021年4月至2022年4月27 408条精液测定记录,采用混合线性分析模型和方差分析探究品种、公猪出生胎次、公猪同窝仔猪数、公猪乳头数、采精季节、采精月龄和采精间隔对精液体积、精液密度、精子活力、直线前进运动精子比例、精子畸形率、总精子数及各精液性状稳定性的影响。【结果】从不同品种对精液品质的影响来看,长白猪精液体积和总精子数均显著高于大白猪、杜洛克猪(P＜0.05),杜洛克猪精液密度显著高于大白猪和长白猪(P＜0.05),杜洛克猪、大白猪精子活力均显著高于长白猪(P＜0.05),大白猪直线前进运动精子比例显著高于长白猪和杜洛克猪(P＜0.05),长白猪和大白猪精子畸形率均显著低于杜洛克猪(P＜0.05);从不同公猪出生胎次对精液品质的影响来看,1~3胎出生的公猪具有较高的精液品质;从不同采精季节对精液品质的影响来看,精液密度、精子活力和总精子数秋、冬季显著高于春、夏季(P＜0.05);从不同采精月龄对精液品质的影响来看,16~25月龄公猪具有较高的精液品质;从不同采精间隔对精液品质的影响来看,4~5 d为最佳采精间隔,采精间隔过长会导致精子畸形率上升;从不同公猪总乳头数来看,乳头数13~16个时,各精液性状品质都处于中等水平,有利于公猪生产应用。3个品种各精液性状间稳定性趋势不一,杜洛克猪和大白猪精液体积和精子活力的稳定性均显著高于长白猪(P＜0.05),杜洛克猪精液密度、精子畸形率和总精子数的稳定性均显著高于大白猪和长白猪(P＜0.05),大白猪直线前进运动精子比例的稳定性显著高于长白猪和杜洛克猪(P＜0.05)。各品种中,长白猪稳定性较差;各精液性状中,精子活力稳定性最好。【结论】品种、公猪出生胎次、采精季节、采精月龄和采精间隔均会影响公猪精液品质,可根据不同品种公猪制定更完善的选择方案,提高精液质量,加速公猪遗传改良。 相似文献
7.
8.
猪精液冷冻保存技术对生猪产业发展有重要意义,有利于提高优秀种公猪的利用率以及实现优秀种质资源的长久保存,促进种猪场降本增效。目前中国猪精液冷冻保存技术取得了一系列进步,国内应用猪冻精配种的受胎率以及产活仔数基本达到了国外冻精配种水平。但是,由于冻融过程会对猪精子造成一定程度的损伤,精子的受精能力下降,受精窗口期缩短,国内、外应用冻精配种的生产成绩仍与常温保存精液存在不小差距。文章回顾了国内、外猪精液冷冻保存技术的发展历程,介绍了猪精液和精子的基本特点,简述了降温过程造成精子损伤的机理,并从冷冻稀释液成分这一角度分析介绍了减小冷冻损伤的机理与方法,如添加冷冻保护剂减少细胞内外冰晶形成、维持细胞内外渗透压以及稳定细胞膜结构;添加抗菌物质抑制解冻后细菌的快速繁殖;添加抗氧化物降低活性氧水平,缓解氧化应激,保护精子结构和受精能力等,最后对猪精液冷冻保存技术未来的发展方向进行展望,以期为相关研究以及实际应用提供参考。 相似文献
9.
试验测定了民猪、巴民杂交1代母猪、丹系大白母猪窝产仔数、窝产活仔数及毛色性状。民猪母猪与巴克夏公猪杂交,母猪平均窝产仔数11.43头,窝产活仔数10.29头;39窝仔猪中,有21窝全窝仔猪全部是黑仔猪,占54%。巴民杂交1代黑色公母猪横交,母猪窝产仔数8.92头,窝产活仔数8.22头;32窝仔猪中,仅有1窝全窝仔猪都是黑色仔猪,占3%。杂交1代黑色母猪与巴克夏公猪级进杂交,母猪平均窝产仔数9.29头,窝产活仔数8.76头;33窝仔猪中,仅有2窝全窝仔猪都是黑色仔猪,占6%。杂交一代黑色母猪与杜洛克公猪杂交,13窝仔猪中,没有出现全窝仔猪都是黑仔猪现象,有3窝仔猪中出现全身为棕色个体（或带黑斑点）。纯繁大白猪窝产仔数11.64头,窝产活仔数10.66头。 相似文献
10.
本文对比大量的实际生产数据，研究了公猪精液不同精子活率对母猪繁殖性能的影响，从所配母猪的受胎情况、分娩情况、窝产总仔和窝产活仔等繁殖成绩综合分析表明：活率在0.4以下时，母猪的受胎率、分娩率、窝均活仔和窝均总仔均远远低于活率0.4以上所配母猪的对应指标，活率0.4～0.6基本达到稳定繁殖成绩，活率0.6以上与活率0.4～0.6的繁殖成绩差异不大。从本研究结果看，要发挥优良公、母猪的最大产能，不必一味追求公猪精液精子活率，而要确保输入有效精子数即可。 相似文献
11.
为研究不同品种、采精月份、采精月龄和采精间隔等因素对加系公猪精液品质的影响,以及品种、初次采精周龄对精液质量稳定性的影响,本研究以江西某种公猪站79头加系大白猪、长白猪、杜洛克猪种公猪为试验群体,收集2018年12月至2020年12月3 921条精液采集与精液质量数据,通过混合线性模型与方差分析探究各因素对精液量、精液密度、精子活力、总精子数及其稳定性的影响。结果显示,从不同品种对精液质量的影响来看,长白猪精液量和总精子数均高于大白猪、杜洛克猪,但杜洛克猪精液密度高于长白猪、大白猪,杜洛克猪精子活力最低;从不同月份来看,1~3月采精精液密度最高,4~6月采精精子活力最高,10~12月采精精液量和总精子数最高,精液量呈现秋冬多、春夏少的季节变化规律。公猪不同月龄采精,精液质量指标也存在差异,月龄越小精液量越低,但精液密度偏高,精子活力相对较好,在19~24月龄黄金期总精子数最高。不同采精间隔对精液质量有较大影响,采精间隔越长,精液量、精液密度、精子活力和总精子数相对较好,采精间隔为5 d时综合性能最佳,但过长的采精间隔导致精子活力降低。品种影响总精子数稳定性,长白猪、大白猪总精子数稳定性显著优于杜洛克猪（P<0.05）。本研究结果表明,品种、采精月份、采精月龄和采精间隔均会影响公猪精液质量,关注这些因素有助于公猪站制定更完善的生产计划,提高公猪利用率。 相似文献
12.
D Ren Y Xing M Lin Y Wu K Li W Li S Yang T Guo J Ren J Ma L Lan L Huang 《Reproduction in domestic animals》2009,44(6):913-919
Chinese Erhualian pigs are known for prolificacy with distinct reproductive traits compared with Western commercial breeds. In this study, a four‐generation intercross resource population was constructed using White Duroc boars and Chinese Erhualian sows as founder animals, and a total of 14 male reproductive traits were recorded in 411 F2/F3 boars including the testis and epididymis weights, the seminiferous tubular diameter and spermatogenesis at 60, 90 and 300 days of age, semen characteristics, serum testosterone concentration and libido level at 300 days of age. The White Duroc–Erhualian boars showed remarkable segregations in the traits measured except for the seminiferous tubular diameter and had high ratio (13.9%) of the abnormality of spermatogenesis, providing a good experimental population for detecting quantitative trait loci affecting these male reproductive traits. Furthermore, the correlations among nine male reproductive traits at 300 days of age indicated that the testis weight and the body weight were strongly correlated with the sperm production, supporting the two traits as important parameters for boar selection to increase sperm production and ultimately improve boar fertility. The libido level in the White Duroc–Erhualian boars that was evaluated by a new and easily recorded scoring system showed a significant correlation with serum testosterone concentration. Yet, both libido and serum testosterone concentration were not correlated with the sperm production. Results of this study provided new information on the male reproductive physiology and genetics in Chinese Erhualian and White Duroc boars. 相似文献
13.
Kaewmala K Uddin MJ Cinar MU Große-Brinkhaus C Jonas E Tesfaye D Phatsara C Tholen E Looft C Schellander K 《Reproduction in domestic animals》2012,47(2):213-223
Phospholipase C zeta (PLCz) and cyclooxygenase isoenzyme type 2 (COX-2) are important in spermatogenesis, but their effect has not yet confirmed in pigs. Therefore, this study was aimed to analyse their association with sperm quality and fertility and to identify the mRNA and protein expression in boars reproductive tissues. DNA samples from 231 Pietrain (PI) and 109 Pietrain × Hampshire (PIHA) pigs with records of sperm quality [sperm concentration (SCON), motility, semen volume, plasma droplet and abnormal spermatozoa rate] and fertility (non-return rate and number of piglet born alive) traits were available. A SNP in non-coding region of PLCz g.158 A > C was associated with SCON (p < 0.05) in PIHA population while the polymorphism of COX-2 g.68 G > A in 3' UTR was not associated with any traits. For mRNA and protein expression study, a total of six boars were divided into two groups with G-I and G-II, where G-I was characterized for relatively better sperm quality. Both genes expressed higher in reproductive tissues compared with non-reproductive tissues. Phospholipase C zeta mRNA expressed higher in testis (p < 0.01), all parts of epididymis and spermatozoa from G-I, while COX-2 expressed higher in testis (p < 0.05), head and body of epididymis (p < 0.01), and spermatozoa from G-II boar. Both proteins were localized in Leydig cells and spermatozoa. These results might shed light on roles of these genes in spermatogenesis as candidate for boar sperm quality and fertility, but still the lack of association across populations should be considered. 相似文献
14.
Krzysztof Górski Stanisław Kondracki Maria Iwanina Władysław Kordan Leyland Fraser 《Animal Science Journal》2021,92(1):e13629
The aim of the study was to determine the relation between the semen quality, frequency of sperm defects, sperm dimensions and shape, and the ejaculate volume of Large White and Landrace boars. A total of 648 ejaculates collected from 31 Large White and 30 Landrace boars were divided into three groups according to the criterion of the ejaculate volume. In this study Landrace boars produced ejaculates with higher volume, sperm concentration, and total numbers of spermatozoa than Large White boars. Landrace boars also showed a lower frequency of sperm with morphological abnormalities (P < 0.05). Landrace boars sperm had larger heads, which were by 0.15 μm longer, and by a larger perimeter and area (P < 0.05). Landrace boar spermatozoa also had a longer flagellum and were generally larger and by 2.07 μm longer than Large White boar sperm (P < 0.05). Significant differences were also found in the shape of sperm of the two breeds (P < 0.05). Landrace boars sperm had more elongated heads, and the ratio of head size to flagellum length was lower than in Large White boars sperm (P < 0.05). Sperm from ejaculates with low volume had a shorter flagellum and a greater head length/flagellum length ratio than sperm from medium- and high-volume ejaculates (P < 0.05). 相似文献
15.
Gunawan A Cinar MU Uddin MJ Kaewmala K Tesfaye D Phatsara C Tholen E Looft C Schellander K 《Reproduction in domestic animals》2012,47(5):782-790
ESR2 is involved in oestrogen-related apoptosis in cell cycle spermatogenesis but their effects have not yet confirmed in pig. Therefore, this study was aimed to investigate the association of ESR2 polymorphism with sperm quality and boar fertility traits and to analyse the ESR2 mRNA and protein expressions in boar reproductive tissues. DNA samples from 203 Pietrain (PI) and 100 Pietrain × Hampshire (PIHA) pigs with records of sperm quality [sperm concentration (SCON), motility (MOT), semen volume (VOL), plasma droplet rate (PDR) and abnormal spermatozoa rate (ASR)] and fertility [non-return rate (NRR) and number of piglet born alive (NBA)] traits were available. A SNP in coding region of ESR2 g.35547A>G in exon 5 was associated with MOT and PDR in the PI and with SCON, VOL, MOT and PDR in PIHA population. For mRNA and protein expression study, a total of six boars were divided into two groups with group I (G-I) and group II (G-II) where G-I characterized for relatively a better sperm quality according to the mean of two groups. mRNA expression was higher in brain and testis than that in all parts of epididymis. Both qRT-PCR and western blot analysis revealed that the ESR2 gene expression and protein expression were significantly higher in testis collected from G-II compared with that of G-I boars. Moreover, ESR2 protein localization in germ cell, Leydig and Sertoli cells, epithelial cells and spermatozoa was remarkable, which indicated the important role of ESR2 in spermatogenesis process. These results might shed new light on the roles of ESR2 in spermatogenesis as candidate for boar fertility, but still the lack of association across populations should be considered. 相似文献
16.
Semen alterations in porcine rubulavirus-infected boars are related to viral excretion and have implications for artificial insemination 总被引：1，自引：1，他引：0
Solís M Ramírez-Mendoza H Mercado C Espinosa S Vallejo V Reyes-Leyva J Hernández J 《Research in veterinary science》2007,83(3):403-409
Porcine rubulavirus (PoRV), also known as blue eye disease (BED) of swine, causes respiratory and reproductive problems in pigs at several developmental stages. To study the effect of PoRV infection on semen production, five boars were infected with 1 x 10(6) TCID(50)/ml of PoRV strain PAC-3 and evaluated for 59 days post inoculation (DPI). Infected boars developed reproductive tract pathology that included swelling of the testes and epididymides. Analysis of the semen showed that the infection had little effect on semen production in four animals, but semen from one boar showed severe alterations in sperm concentration, motility, and morphology. When motility was analyzed in BTS-diluted semen after 24, 48, or 72 h, alterations were detected in all boars. Furthermore, viral antigen was detected in semen, the seminal plasma fraction, or sperm fraction from all boars. These results showed that PoRV is excreted via semen and, therefore, artificial insemination is a potential route of dissemination. 相似文献
17.
Assessment of reproduction and growth performance of offspring derived from somatic cell cloned pigs
Zeping ZHAO Xinyu LU Biao LIU Yutian LI Hongbin WANG Zhonghua LIU 《Animal Science Journal》2012,83(9):639-643
Since cloned pig was successfully produced, a new opportunity for porcine breeding industry to conserve genetic resources has been opened. However, there has been no report to investigate whether both somatic cell nuclear transfer (SCNT) pigs and their offspring have the characteristics of the donor breed. In this study, we compared the reproductive and growth performance of American Large White boars cloned by SCNT with the donor boar, and analyzed the test parameters, including semen quality, re‐service rate, rate of parturition, and average daily gain. The results showed that these cloned boars and the donor boar had no significant differences in the tests (P > 0.05) and the growth performance of their offspring was similar to the naturally bred American Large White pigs. In summary, the reproductive and growth performance of cloned pigs are similar to the donor pig and within the normal range. This suggests that pigs cloned by SCNT have the potential to be used in reproduction and breeding. 相似文献
18.
Shinichiro Ogawa Ayane Konta Makoto Kimata Kazuo Ishii Yoshinobu Uemoto Masahiro Satoh 《Animal Science Journal》2019,90(1):23-28
Genetic parameters were estimated for six reproductive traits related to farrowing events in Landrace and Large White pigs; total number born (TNB), number born alive (NBA), number stillborn (NSB), total litter weight at birth (LWB), mean litter weight at birth (MWB), and gestation length (GL). We analyzed 62,534 farrowing records for 10,637 Landrace dams and 49,817 farrowing records for 8,649 Large White dams. Estimated heritabilities of TNB, NBA, NSB, LWB, MWB, and GL by single‐trait repeatability model analyses were 0.12, 0.12, 0.08, 0.18, 0.19, and 0.29, respectively, in Landrace, and 0.12, 0.10, 0.08, 0.18, 0.16, and 0.34, respectively, in Large White. Genetic correlation between NBA and NSB was unfavorable: 0.20 in Landrace and 0.33 in Large White. Genetic correlations of GL with the other five traits were weak: from ?0.18 with NSB to ?0.03 with NBA in Landrace, and from ?0.22 with NSB to ?0.07 with NBA in Large White. LWB had a highly favorable genetic correlation with NBA (0.74 in both breeds), indicating the possibility of using LWB for the genetic improvement of NBA. 相似文献