共查询到18条相似文献,搜索用时 203 毫秒
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旨在基于公、母牛性信息素嗅觉交流的事实,为安静发情鉴定技术的研发提供重要参考。本研究预选择石家庄天泉良种奶牛有限公司的25头3~5周龄健康、产后15~35 d的可繁荷斯坦母牛,联合使用人工观察、活动量监测、直肠检查和B超检测等方法,对荷斯坦母牛进行发情鉴定,并区分正常发情和安静发情,共采集到4只正常发情和5只安静发情母牛的不同发情阶段的唾液样品,后利用固相微萃取-气相色谱-质谱联用仪(SPME-GC-MS),鉴定分析这些样品中的挥发性化合物成分,并比较不同发情状态及不同发情阶段的差异。结果表明,发现40个在所有母牛唾液中都存在的化合物,经过配对分析,从中筛选出23个发情时含量显著高于发情前的化合物(P<0.05),其中,13个化合物(1-戊醇、吡咯、1-己醇、3-己烯-1-醇、苯乙醇、6-甲基-2-庚酮、6-(2,2,2-三氯-1-羟乙基)-1-甲基环己烯和2-戊基呋喃、2-甲基-1-戊醇、2,6,6-三甲基-2-环己烯-1,4-二酮、3-甲基-1-丁醇、二甲基砜、亚磺酰基双甲烷)在所有母牛发情时含量均有升高趋势。这些化合物可作为发情候选标记用于母牛发情鉴定,并且其含量在正常发情... 相似文献
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高效发情鉴定是提高母牛繁殖力、提升牛场经济效益的重要途径。随着奶牛养殖规模越来越大,传统人工观察已难以适应规模场奶牛发情鉴定需要。计步器发情鉴定难以检出安静发情,无法提高发情检出率。虽然体温参数发情鉴定潜力大,但母牛全身被毛,体表测温不理想,而接触式测温采集部位有限,可穿戴设备研发难度较大,不同采集部位牛只体温数据差别很大,为此,本文综述了前人利用奶牛躯体不同部位体温数据发情鉴定的结果,比较分析了发情奶牛各部位体温变化规律与发情的关系,为研发高效母牛发情鉴定技术提供科学参考。 相似文献
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母牛适时输精关键在于发情鉴定的准确与否。笔者根据触摸母牛卵巢卵泡的发育和子宫颈变化做母牛发情鉴定和并据此确定输精时机,提高了母牛受胎率。现将具体情况报告如下。一、母牛选择1982年以来选用泰康镇奶牛场及养牛户的5405头可繁母牛、改良牛及黄牛为研究对象。二、方法1982年以来笔者在采用直肠把握式输精过程中,发现了母牛在发情期子宫颈也在发生周期性变化,根据子宫颈的变化规 相似文献
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同期发情技术可以有效地避免反复多次的母牛发情鉴定,提高母牛同期发情率,通过同期发情达到直接定时授精的目的.研究母牛同期发情技术对于了解不同的发情技术及其具体应用至关重要,同时对于促进母牛同期发情技术的创新发展以及提高母牛繁殖率具有很强的现实意义. 相似文献
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为探究同期排卵处理母牛体温和活动量变化规律及不同同期排卵技术处理效果,指导同期排卵技术优化。本研究自动监测了18头20月龄左右同期排卵(GnRH-PG-GnRH)处理荷斯坦母牛和17头产后40~60 d预同期排卵(PG-PG-GnRH-PG-GnRH)处理荷斯坦母牛的体温和活动量,应用自动检测系统进行母牛发情监测。结果发现,同期排卵处理母牛发情时阴道温度平均升高(0.43±0.20)℃,持续(12.37±2.73)h;活动量平均升高(18.28±18.61)倍,持续(11.00±1.68)h;排卵时阴道温度平均下降(0.20±0.10)℃,持续(11.00±1.68)h。自动化发情监测显示,同期排卵处理母牛7头发情并排卵;预同期排卵母牛GnRH处理前全部发情排卵。两种同期排卵处理,虽可改变母牛性周期进程,促进母牛性周期同步化,但均难以使母牛性周期完全同步。因此,将同期排卵-定时输精和发情鉴定技术科学结合才能取得更好的繁殖效果。 相似文献
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本研究应用盐酸山莨菪碱(150mg)及微量的雌激素(4mg)对1~4胎90天以上未出现发情的不孕奶牛45例进行催情,其有效率为71.11%(32/45),其中卵巢静止为60%(15/25),持久黄体85%(17/20)。同时还发现用上述两种药物处理的10例患牛的血清孕酮含量在注射后的120小时以内均有程度不同的下降;当血清孕酮最低峰值<1.0ng/ml时,临床呈现发情征象的持久黄体牛为83.3%(5/6);卵巢静止牛为100%(4/4)。5例仅用微量雌激素(4mg)注射的患牛孕酮含量虽有变化但临床上均不出现显性发情症状。初步证明:盐酸山莨菪碱与微量雌激素协同可对奶牛有明显的催情作用,此方法迄今国内外未见报道。 相似文献
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Estrous expressions in dairy cows have been shortened and weakened. Dairy heifers, on the other hand, may not have had such changes in estrous signs as observed in cows, since they have less stresses than cows. The aim of this study was to describe the duration of estrus in a herd of dairy heifers. A total of 56 Holstein Friesian heifers estrus was synchronized using two different hormonal protocols. They were checked for primary and secondary estrous signs with the help of heat detection devices for 48 h at an interval of 4 h starting at 16.00 hour, one day after PGF2α treatment. Onset and end of standing estrus during 48 h observation period was recorded in 35 of the 44 heifers coming into estrus within 5 days after PGF2α treatment during the observation period. The duration of standing estrus on the average (±SD) was 9.7 ± 5.3 h. Percentage of heifers with standing estrus longer than 12 h was 40%, and 53% showed standing estrus only for 4–8 h. It is indicated that duration of estrus in dairy heifers has been shortened recently. 相似文献
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Efficiency of a pedometer device for detecting estrus in standing heat and silent heat in Japanese Black cattle
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The usefulness of a radiotelemetric pedometer for estrus detection in standing (ST) heat, or in silent heat without ST events, but in which ovulation is observed, in Japanese Black cattle was investigated. The duration of an increase in steps in ST heat was 11.8 ± 1.3 hr, and it was similar to that of ST events (duration: 10.1 ± 0.8 hr). Even in silent heat, the change pattern and the duration (11.6 ± 0.2 hr) of the period with an increase in steps during estrus were not different compared with ST heat. When artificial insemination (AI) was performed at 15.5 ± 0.6 hr from the onset of estrus detected by the pedometer in ST heat cases, the conception rate was 57.1% (8/14). Furthermore, fertility in cattle that underwent silent heat was evaluated. When AI was performed at 14.4 ± 2.0 hr from the onset of estrus detected by the pedometer, the conception rate was 60% (3/5) in silent heat cases. The overall results suggest that the radiotelemetric pedometer is a valid device for detecting estrus and it can even detect silent heat in Japanese Black cattle. Moreover, even silent heat cattle are fertile when AI is performed at the appropriate time. 相似文献